Isolated polynucleotides and polypeptides, and methods of using same for increasing plant yield and/or agricultural characteristics

ABSTRACT

Provided are isolated polypeptides which are at least 80% homologous to SEQ ID NO: 474-643, 645-679, 681-755, 757-760, 4806-6390, 6395-6396, 6401-6895, 6897-7249, 7251-7685, 7687-7693, 7695-7700, 7702-7708, 7710-7796, 7798-7816, 7818, 7820-7837, 7839-7840, 7842-7861, 7863-8134, 8136-8163 or 8164, isolated polynucleotides which are at least 80% identical to SEQ ID NOs: 1-170, 172-267, 269-424, 426-473, 761-2486, 2489-2494, 2496-4803 or 4804, nucleic acid constructs comprising same, transgenic cells expressing same, transgenic plants expressing same and method of using same for increasing yield, harvest index, abiotic stress tolerance, growth rate, biomass, vigor, oil content, photosynthetic capacity, seed yield, fiber yield, fiber quality, fiber length, and/or nitrogen use efficiency of a plant.

RELATED APPLICATIONS

This application is a division of U.S. patent application Ser. No.16/210,035 filed on Dec. 5, 2018, which is a division of U.S. patentapplication Ser. No. 15/865,405 filed on Jan. 9, 2018, now U.S. Pat. No.10,214,748, which is a division of U.S. patent application Ser. No.14/787,037 filed on Oct. 26, 2015, now U.S. Pat. No. 9,920,329, which isa National Phase of PCT Patent Application No. PCT/IL2014/050454 having15 International Filing Date of May 21, 2014, which claims the benefitof priority under 35 USC § 119(e) of U.S. Provisional PatentApplications Nos. 61/893,391 filed on Oct. 21, 2013, 61/870,380 filed onAug. 27, 2013 and 61/826,066 filed on May 22, 2013. The contents of theabove applications are all incorporated by reference as if fully setforth herein in their entirety.

SEQUENCE LISTING STATEMENT

The ASCII file, entitled 84759SequenceListing.txt, created on Nov. 4,2020, comprising 19,201,058 bytes, submitted concurrently with thefiling of this application is incorporated herein by reference.

FIELD AND BACKGROUND OF THE INVENTION

The present invention, in some embodiments thereof, relates to isolatedpolypeptides and polynucleotides, nucleic acid constructs comprisingsame, transgenic cells comprising same, transgenic plants transformedwith said nucleic acid constructs and transgenic plants exogenouslyexpressing same and more particularly, but not exclusively, to methodsof using same for increasing yield (e.g., seed yield, oil yield, harvestindex), biomass, photosynthetic capacity (e.g., leaf area), growth rate,vigor, oil content, fiber yield, fiber quality, fertilizer useefficiency (e.g., nitrogen use efficiency) and/or abiotic stresstolerance of a plant.

Yield is affected by various factors, such as, the number and size ofthe plant organs, plant architecture (for example, the number ofbranches), grains set length, number of filled grains, vigor (e.g.seedling), growth rate, root development, utilization of water,nutrients (e.g., nitrogen) and fertilizers, and stress tolerance.

Crops such as, corn, rice, wheat, canola and soybean account for overhalf of total human caloric intake, whether through direct consumptionof the seeds themselves or through consumption of meat products raisedon processed seeds or forage. Seeds are also a source of sugars,proteins and oils and metabolites used in industrial processes. Theability to increase plant yield, whether through increase dry matteraccumulation rate, modifying cellulose or lignin composition, increasestalk strength, enlarge meristem size, change of plant branchingpattern, erectness of leaves, increase in fertilization efficiency,enhanced seed dry matter accumulation rate, modification of seeddevelopment, enhanced seed filling or by increasing the content of oil,starch or protein in the seeds would have many applications inagricultural and non-agricultural uses such as in the biotechnologicalproduction of pharmaceuticals, antibodies or vaccines.

Vegetable or seed oils are the major source of energy and nutrition inhuman and animal diet. They are also used for the production ofindustrial products, such as paints, inks and lubricants. In addition,plant oils represent renewable sources of long-chain hydrocarbons, whichcan be used as fuel. Since the currently used fossil fuels are finiteresources and are gradually being depleted, fast growing biomass cropsmay be used as alternative fuels or for energy feedstock and may reducethe dependence on fossil energy supplies. However, the major bottleneckfor increasing consumption of plant oils as bio-fuel is the oil price,which is still higher than fossil fuel. In addition, the production rateof plant oil is limited by the availability of agricultural land andwater. Thus, increasing plant oil yields from the same growing area caneffectively overcome the shortage in production space and can decreasevegetable oil prices at the same time.

Studies aiming at increasing plant oil yields focus on theidentification of genes involved in oil metabolism as well as in genescapable of increasing plant and seed yields in transgenic plants. Genesknown to be involved in increasing plant oil yields include thoseparticipating in fatty acid synthesis or sequestering such as desaturase[e.g., DELTA6, DELTA12 or acyl-ACP (Ssi2; Arabidopsis InformationResource (TAIR; arabidopsis (dot) org/). TAIR No. AT2G43710)]. OleosinA(TAIR No. AT3G01570) or FAD3 (TAIR No. AT2G29980), and varioustranscription factors and activators such as Lec1 [TAIR No. AT1G21970,Lotan et al. 1998. Cell. 26; 93(7):1195-205], Lec2 [TAIR No. AT1G28300,Santos Mendoza et al. 2005. FEBS Lett. 579(21):4666-70]. Fus3 (TAIR No.AT3G26790). ABI3 [TAIR No. AT3G24650. Lara et al. 2003. J Biol Chem.278(23): 21003-11] and Wri1 [TAIR No. AT3G54320, Cernac and Benning,2004. Plant J. 40(4): 575-85].

Genetic engineering efforts aiming at increasing oil content in plants(e.g., in seeds) include upregulating endoplasmic reticulum (FAD3) andplastidal (FAD7) fatty acid desaturases in potato (Zabrouskov V., etal., 2002; Physiol Plant, 116:172-185); over-expressing the GmDof4 andGmDof11 transcription factors (Wang H W et al., 2007; Plant J.52:716-29); over-expressing a yeast glycerol-3-phosphate dehydrogenaseunder the control of a seed-specific promoter (Vigeolas H. et al. 2007,Plant Biotechnol J. 5:431-41; U.S. Pat. Appl. No. 20060168684); usingArabidopsis FAE1 and yeast SLC1-1 genes for improvements in erucic acidand oil content in rapeseed (Katavic V, et al., 2000. Biochem Soc Trans.28:935-7).

Various patent applications disclose genes and proteins, which canincrease oil content in plants. These include for example, U.S. Pat.Appl. No. 20080076179 (lipid metabolism protein); U.S. Pat. Appl. No.20060206961 (the Ypr140w polypeptide); U.S. Pat. Appl. No. 20060174373[triacylglycerols synthesis enhancing protein (TEP)]; U.S. Pat. Appl.Nos. 20070169219, 20070006345, 20070006346 and 20060195943 (disclosetransgenic plants with improved nitrogen use efficiency which can beused for the conversion into fuel or chemical feedstocks); WO2008/122980(polynucleotides for increasing oil content, growth rate, biomass, yieldand/or vigor of a plant).

A common approach to promote plant growth has been, and continues to be,the use of natural as well as synthetic nutrients (fertilizers). Thus,fertilizers are the fuel behind the “green revolution”, directlyresponsible for the exceptional increase in crop yields during the last40 years, and are considered the number one overhead expense inagriculture. For example, inorganic nitrogenous fertilizers such asammonium nitrate, potassium nitrate, or urea, typically accounts for 40%of the costs associated with crops such as corn and wheat. Of the threemacronutrients provided as main fertilizers [Nitrogen (N). Phosphate (P)and Potassium (K)], nitrogen is often the rate-limiting element in plantgrowth and all field crops have a fundamental dependence on inorganicnitrogenous fertilizer. Nitrogen is responsible for biosynthesis ofamino and nucleic acids, prosthetic groups, plant hormones, plantchemical defenses, etc. and usually needs to be replenished every year,particularly for cereals, which comprise more than half of thecultivated areas worldwide. Thus, nitrogen is translocated to the shoot,where it is stored in the leaves and stalk during the rapid step ofplant development and up until flowering. In corn for example, plantsaccumulate the bulk of their organic nitrogen during the period of graingermination, and until flowering. Once fertilization of the plant hasoccurred, grains begin to form and become the main sink of plantnitrogen. The stored nitrogen can be then redistributed from the leavesand stalk that served as storage compartments until grain formation.

Since fertilizer is rapidly depleted from most soil types, it must besupplied to growing crops two or three times during the growing season.In addition, the low nitrogen use efficiency (NUE) of the main crops(e.g., in the range of only 30-70%) negatively affects the inputexpenses for the farmer, due to the excess fertilizer applied. Moreover,the over and inefficient use of fertilizers are major factorsresponsible for environmental problems such as eutrophication ofgroundwater, lakes, rivers and seas, nitrate pollution in drinking waterwhich can cause methemoglobinemia, phosphate pollution, atmosphericpollution and the like. However, in spite of the negative impact offertilizers on the environment, and the limits on fertilizer use, whichhave been legislated in several countries, the use of fertilizers isexpected to increase in order to support food and fiber production forrapid population growth on limited land resources. For example, it hasbeen estimated that by 2050, more than 150 million tons of nitrogenousfertilizer will be used worldwide annually.

Increased use efficiency of nitrogen by plants should enable crops to becultivated with lower fertilizer input, or alternatively to becultivated on soils of poorer quality and would therefore havesignificant economic impact in both developed and developingagricultural systems.

Genetic improvement of fertilizer use efficiency (FUE) in plants can begenerated either via traditional breeding or via genetic engineering.

Attempts to generate plants with increased FUE have been described inU.S. Pat. Appl. No. 20020046419 to Choo, et al.; U.S. Pat. Appl. No.20050108791 to Edgerton et al.; U.S. Pat. Appl. No. 20060179511 toChomet et al.; Good. A, et al. 2007 (Engineering nitrogen use efficiencywith alanine aminotransferase. Canadian Journal of Botany 85: 252-262);and Good A G et al. 2004 (Trends Plant Sci. 9:597-605).

Yanagisawa et al. (Proc. Natl. Acad. Sci. U.S.A. 2004 101:7833-8)describe Dof1 transgenic plants, which exhibit improved growth underlow-nitrogen conditions.

U.S. Pat. No. 6,084,153 to Good et al. discloses the use of a stressresponsive promoter to control the expression of Alanine AmineTransferase (AlaAT) and transgenic canola plants with improved droughtand nitrogen deficiency tolerance when compared to control plants.

Abiotic stress (ABS; also referred to as “environmental stress”)conditions such as salinity, drought, flood, suboptimal temperature andtoxic chemical pollution, cause substantial damage to agriculturalplants. Most plants have evolved strategies to protect themselvesagainst these conditions. However, if the severity and duration of thestress conditions are too great, the effects on plant development,growth and yield of most crop plants are profound. Furthermore, most ofthe crop plants are highly susceptible to abiotic stress and thusnecessitate optimal growth conditions for commercial crop yields.Continuous exposure to stress causes major alterations in the plantmetabolism, which ultimately leads to cell death and consequently yieldlosses.

Drought is a gradual phenomenon, which involves periods of abnormallydry weather that persists long enough to produce serious hydrologicimbalances such as crop damage, water supply shortage and increasedsusceptibility to various diseases. In severe cases, drought can lastmany years and results in devastating effects on agriculture and watersupplies. Furthermore, drought is associated with increasesusceptibility to various diseases.

For most crop plants, the land regions of the world are too arid. Inaddition, overuse of available water results in increased loss ofagriculturally usable land (desertification), and increase of saltaccumulation in soils adds to the loss of available water in soils.

Salinity, high salt levels, affects one in five hectares of irrigatedland. None of the top five food crops, i.e., wheat, corn, rice,potatoes, and soybean, can tolerate excessive salt. Detrimental effectsof salt on plants result from both water deficit, which leads to osmoticstress (similar to drought stress), and the effect of excess sodium ionson critical biochemical processes. As with freezing and drought, highsalt causes water deficit; and the presence of high salt makes itdifficult for plant roots to extract water from their environment. Soilsalinity is thus one of the more important variables that determinewhether a plant may thrive. In many parts of the world, sizable landareas are uncultivable due to naturally high soil salinity. Thus,salination of soils that are used for agricultural production is asignificant and increasing problem in regions that rely heavily onagriculture, and is worsen by over-utilization, over-fertilization andwater shortage, typically caused by climatic change and the demands ofincreasing population. Salt tolerance is of particular importance earlyin a plant's lifecycle, since evaporation from the soil surface causesupward water movement, and salt accumulates in the upper soil layerwhere the seeds are placed. On the other hand, germination normallytakes place at a salt concentration, which is higher than the mean saltlevel in the whole soil profile.

Salt and drought stress signal transduction consist of ionic and osmotichomeostasis signaling pathways. The ionic aspect of salt stress issignaled via the SOS pathway where a calcium-responsive SOS3-SOS2protein kinase complex controls the expression and activity of iontransporters such as SOS1. The osmotic component of salt stress involvescomplex plant reactions that overlap with drought and/or cold stressresponses.

Suboptimal temperatures affect plant growth and development through thewhole plant life cycle. Thus, low temperatures reduce germination rateand high temperatures result in leaf necrosis. In addition, matureplants that are exposed to excess of heat may experience heat shock,which may arise in various organs, including leaves and particularlyfruit, when transpiration is insufficient to overcome heat stress. Heatalso damages cellular structures, including organelles and cytoskeleton,and impairs membrane function. Heat shock may produce a decrease inoverall protein synthesis, accompanied by expression of heat shockproteins, e.g., chaperones, which are involved in refolding proteinsdenatured by heat. High-temperature damage to pollen almost alwaysoccurs in conjunction with drought stress, and rarely occurs underwell-watered conditions. Combined stress can alter plant metabolism innovel ways. Excessive chilling conditions, e.g., low, but abovefreezing, temperatures affect crops of tropical origins, such assoybean, rice, maize, and cotton. Typical chilling damage includeswilting, necrosis, chlorosis or leakage of ions from cell membranes. Theunderlying mechanisms of chilling sensitivity are not completelyunderstood yet, but probably involve the level of membrane saturationand other physiological deficiencies. Excessive light conditions, whichoccur under clear atmospheric conditions subsequent to cold latesummer/autumn nights, can lead to photoinhibition of photosynthesis(disruption of photosynthesis). In addition, chilling may lead to yieldlosses and lower product quality through the delayed ripening of maize.

Common aspects of drought, cold and salt stress response [Reviewed inXiong and Zhu (2002) Plant Cell Environ. 25: 131-139] include: (a)transient changes in the cytoplasmic calcium levels early in thesignaling event; (b) signal transduction via mitogen-activated and/orcalcium dependent protein kinases (CDPKs) and protein phosphatases; (c)increases in abscisic acid levels in response to stress triggering asubset of responses; (d) inositol phosphates as signal molecules (atleast for a subset of the stress responsive transcriptional changes; (e)activation of phospholipases which in turn generates a diverse array ofsecond messenger molecules, some of which might regulate the activity ofstress responsive kinases; (f) induction of late embryogenesis abundant(LEA) type genes including the CRT/DRE responsive COR/RD genes; (g)increased levels of antioxidants and compatible osmolytes such asproline and soluble sugars; and (h) accumulation of reactive oxygenspecies such as superoxide, hydrogen peroxide, and hydroxyl radicals.Abscisic acid biosynthesis is regulated by osmotic stress at multiplesteps. Both ABA-dependent and -independent osmotic stress signalingfirst modify constitutively expressed transcription factors, leading tothe expression of early response transcriptional activators, which thenactivate downstream stress tolerance effector genes.

Several genes which increase tolerance to cold or salt stress can alsoimprove drought stress protection, these include for example, thetranscription factor AtCBF/DREB1, OsCDPK7 (Saijo et al. 2000, Plant J.23: 319-327) or AVP1 (a vacuolar pyrophosphatase-proton pump, Gaxiola etal. 2001, Proc. Natl. Acad. Sci. USA 98:11444-11449).

Studies have shown that plant adaptations to adverse environmentalconditions are complex genetic traits with polygenic nature.Conventional means for crop and horticultural improvements utilizeselective breeding techniques to identify plants having desirablecharacteristics. However, selective breeding is tedious, time consumingand has an unpredictable outcome. Furthermore, limited germplasmresources for yield improvement and incompatibility in crosses betweendistantly related plant species represent significant problemsencountered in conventional breeding. Advances in genetic engineeringhave allowed mankind to modify the germplasm of plants by expression ofgenes-of-interest in plants. Such a technology has the capacity togenerate crops or plants with improved economic, agronomic orhorticultural traits.

Genetic engineering efforts, aimed at conferring abiotic stresstolerance to transgenic crops, have been described in variouspublications [Apse and Blumwald (Curr Opin Biotechnol. 13:146-150.2002).Quesada et al. (Plant Physiol. 130:951-963, 2002), Holmström et al.(Nature 379: 683-684, 1996). Xu et al. (Plant Physiol 110: 249-257,1996), Pilon-Smits and Ebskamp (Plant Physiol 107: 125-130, 1995) andTarczynski et al. (Science 259: 508-510, 1993)].

Various patents and patent applications disclose genes and proteins,which can be used for increasing tolerance of plants to abioticstresses. These include for example, U.S. Pat. Nos. 5,296,462 and5,356,816 (for increasing tolerance to cold stress); U.S. Pat. No.6,670,528 (for increasing ABST); U.S. Pat. No. 6,720,477 (for increasingABST); U.S. application Ser. Nos. 09/938,842 and 10/342,224 (forincreasing ABST); U.S. application Ser. No. 10/231,035 (for increasingABST); WO2004/104162 (for increasing ABST and biomass); WO2007/020638(for increasing ABST, biomass, vigor and/or yield); WO2007/049275 (forincreasing ABST, biomass, vigor and/or yield); WO2010/076756 (forincreasing ABST, biomass and/or yield); WO2009/083958 (for increasingwater use efficiency, fertilizer use efficiency, biotic/abiotic stresstolerance, yield and/or biomass); WO2010/020941 (for increasing nitrogenuse efficiency, abiotic stress tolerance, yield and/or biomass);WO2009/141824 (for increasing plant utility); WO2010/049897 (forincreasing plant yield).

Nutrient deficiencies cause adaptations of the root architecture,particularly notably for example is the root proliferation withinnutrient rich patches to increase nutrient uptake. Nutrient deficienciescause also the activation of plant metabolic pathways, which maximizethe absorption, assimilation and distribution processes such as byactivating architectural changes. Engineering the expression of thetriggered genes may cause the plant to exhibit the architectural changesand enhanced metabolism also under other conditions.

In addition, it is widely known that the plants usually respond to waterdeficiency by creating a deeper root system that allows access tomoisture located in deeper soil layers. Triggering this effect willallow the plants to access nutrients and water located in deeper soilhorizons particularly those readily dissolved in water like nitrates.

Cotton and cotton by-products provide raw materials that are used toproduce a wealth of consumer-based products in addition to textilesincluding cotton foodstuffs, livestock feed, fertilizer and paper. Theproduction, marketing, consumption and trade of cotton-based productsgenerate an excess of $100 billion annually in the U.S. alone, makingcotton the number one value-added crop.

Even though 90% of cotton's value as a crop resides in the fiber (lint),yield and fiber quality has declined due to general erosion in geneticdiversity of cotton varieties, and an increased vulnerability of thecrop to environmental conditions.

There are many varieties of cotton plant, from which cotton fibers witha range of characteristics can be obtained and used for variousapplications. Cotton fibers may be characterized according to a varietyof properties, some of which are considered highly desirable within thetextile industry for the production of increasingly high qualityproducts and optimal exploitation of modem spinning technologies.

Commercially desirable properties include length, length uniformity,fineness, maturity ratio, decreased fuzz fiber production, micronaire,bundle strength, and single fiber strength. Much effort has been putinto the improvement of the characteristics of cotton fibers mainlyfocusing on fiber length and fiber fineness. In particular, there is agreat demand for cotton fibers of specific lengths.

A cotton fiber is composed of a single cell that has differentiated froman epidermal cell of the seed coat, developing through four stages,i.e., initiation, elongation, secondary cell wall thickening andmaturation stages. More specifically, the elongation of a cotton fibercommences in the epidermal cell of the ovule immediately followingflowering, after which the cotton fiber rapidly elongates forapproximately 21 days. Fiber elongation is then terminated, and asecondary cell wall is formed and grown through maturation to become amature cotton fiber.

Several candidate genes which are associated with the elongation,formation, quality and yield of cotton fibers were disclosed in variouspatent applications such as U.S. Pat. No. 5,880,100 and U.S. patentapplication Ser. Nos. 08/580,545, 08/867,484 and 09/262,653 (describinggenes involved in cotton fiber elongation stage); WO0245485 (improvingfiber quality by modulating sucrose synthase); U.S. Pat. No. 6,472,588and WO0117333 (increasing fiber quality by transformation with a DNAencoding sucrose phosphate synthase); WO9508914 (using a fiber-specificpromoter and a coding sequence encoding cotton peroxidase); WO9626639(using an ovary specific promoter sequence to express plant growthmodifying hormones in cotton ovule tissue, for altering fiber qualitycharacteristics such as fiber dimension and strength); U.S. Pat. Nos.5,981,834, 5,597,718, 5,620,882, 5,521,708 and 5,495,070 (codingsequences to alter the fiber characteristics of transgenic fiberproducing plants); U.S. patent applications U.S. 2002049999 and U.S.2003074697 (expressing a gene coding for endoxyloglucan transferase,catalase or peroxidase for improving cotton fiber characteristics); WO01/40250 (improving cotton fiber quality by modulating transcriptionfactor gene expression); WO 96/40924 (a cotton fiber transcriptionalinitiation regulatory region associated which is expressed in cottonfiber); EP0834566 (a gene which controls the fiber formation mechanismin cotton plant); WO2005/121364 (improving cotton fiber quality bymodulating gene expression); WO2008/075364 (improving fiber quality,yield/biomass/vigor and/or abiotic stress tolerance of plants).

WO publication No. 2004/104162 discloses methods of increasing abioticstress tolerance and/or biomass in plants and plants generated thereby.

WO publication No. 2004/111183 discloses nucleotide sequences forregulating gene expression in plant trichomes and constructs and methodsutilizing same.

WO publication No. 2004/081173 discloses novel plant derived regulatorysequences and constructs and methods of using such sequences fordirecting expression of exogenous polynucleotide sequences in plants.

WO publication No. 2005/121364 discloses polynucleotides andpolypeptides involved in plant fiber development and methods of usingsame for improving fiber quality, yield and/or biomass of a fiberproducing plant.

WO publication No. 2007/049275 discloses isolated polypeptides,polynucleotides encoding same, transgenic plants expressing same andmethods of using same for increasing fertilizer use efficiency, plantabiotic stress tolerance and biomass.

WO publication No. 2007/020638 discloses methods of increasing abioticstress tolerance and/or biomass in plants and plants generated thereby.

WO publication No. 2008/122980 discloses genes constructs and methodsfor increasing oil content, growth rate and biomass of plants.

WO publication No. 2008/075364 discloses polynucleotides involved inplant fiber development and methods of using same.

WO publication No. 2009/083958 discloses methods of increasing water useefficiency, fertilizer use efficiency, biotic/abiotic stress tolerance,yield and biomass in plant and plants generated thereby.

WO publication No. 2009/141824 discloses isolated polynucleotides andmethods using same for increasing plant utility.

WO publication No. 2009/013750 discloses genes, constructs and methodsof increasing abiotic stress tolerance, biomass and/or yield in plantsgenerated thereby.

WO publication No. 2010/020941 discloses methods of increasing nitrogenuse efficiency, abiotic stress tolerance, yield and biomass in plantsand plants generated thereby.

WO publication No. 2010/076756 discloses isolated polynucleotides forincreasing abiotic stress tolerance, yield, biomass, growth rate, vigor,oil content, fiber yield, fiber quality, and/or nitrogen use efficiencyof a plant.

WO2010/100595 publication discloses isolated polynucleotides andpolypeptides, and methods of using same for increasing plant yieldand/or agricultural characteristics.

WO publication No. 2010/049897 discloses isolated polynucleotides andpolypeptides and methods of using same for increasing plant yield,biomass, growth rate, vigor, oil content, abiotic stress tolerance ofplants and nitrogen use efficiency.

WO2010/143138 publication discloses isolated polynucleotides andpolypeptides, and methods of using same for increasing nitrogen useefficiency, fertilizer use efficiency, yield, growth rate, vigor,biomass, oil content, abiotic stress tolerance and/or water useefficiency WO publication No. 2011/080674 discloses isolatedpolynucleotides and polypeptides and methods of using same forincreasing plant yield, biomass, growth rate, vigor, oil content,abiotic stress tolerance of plants and nitrogen use efficiency.

WO2011/015985 publication discloses polynucleotides and polypeptides forincreasing desirable plant qualities.

WO2011/135527 publication discloses isolated polynucleotides andpolypeptides for increasing plant yield and/or agriculturalcharacteristics.

WO2012/028993 publication discloses isolated polynucleotides andpolypeptides, and methods of using same for increasing nitrogen useefficiency, yield, growth rate, vigor, biomass, oil content, and/orabiotic stress tolerance.

WO2012/085862 publication discloses isolated polynucleotides andpolypeptides, and methods of using same for improving plant properties.

WO2012/150598 publication discloses isolated polynucleotides andpolypeptides and methods of using same for increasing plant yield,biomass, growth rate, vigor, oil content, abiotic stress tolerance ofplants and nitrogen use efficiency.

WO2013/027223 publication discloses isolated polynucleotides andpolypeptides, and methods of using same for increasing plant yieldand/or agricultural characteristics.

WO2013/080203 publication discloses isolated polynucleotides andpolypeptides, and methods of using same for increasing nitrogen useefficiency, yield, growth rate, vigor, biomass, oil content, and/orabiotic stress tolerance.

WO2013/098819 publication discloses isolated polynucleotides andpolypeptides, and methods of using same for increasing yield of plants.

WO2013/128448 publication discloses isolated polynucleotides andpolypeptides and methods of using same for increasing plant yield,biomass, growth rate, vigor, oil content, abiotic stress tolerance ofplants and nitrogen use efficiency.

WO 2013/179211 publication discloses isolated polynucleotides andpolypeptides, and methods of using same for increasing plant yieldand/or agricultural characteristics.

WO2014/033714 publication discloses isolated polynucleotides,polypeptides and methods of using same for increasing abiotic stresstolerance, biomass and yield of plants.

SUMMARY OF THE INVENTION

According to an aspect of some embodiments of the present inventionthere is provided a method of increasing yield, harvest index, growthrate, biomass, vigor, oil content, seed yield, fiber yield, fiberquality fiber length, photosynthetic capacity, nitrogen use efficiency,and/or abiotic stress tolerance of a plant, comprising expressing withinthe plant an exogenous polynucleotide comprising a nucleic acid sequenceencoding a polypeptide at least 80% homologous (e.g., identical) to SEQID NO: 474-643, 645-679, 681-755, 757-760, 4806-6390, 6395-6396,6401-6895, 6897-7249, 7251-7685, 7687-7693, 7695-7700, 7702-7708,7710-7796, 7798-7816, 7818, 7820-7837, 7839-7840, 7842-7861, 7863-8134,8136-8163 or 8164, thereby increasing the yield, harvest index, growthrate, biomass, vigor, oil content, seed yield, fiber yield, fiberquality, fiber length, photosynthetic capacity, nitrogen use efficiency,and/or abiotic stress tolerance of the plant.

According to an aspect of some embodiments of the present inventionthere is provided a method of increasing yield, harvest index, growthrate, biomass, vigor, oil content, seed yield, fiber yield, fiberquality, fiber length, photosynthetic capacity, nitrogen use efficiency,and/or abiotic stress tolerance of a plant, comprising expressing withinthe plant an exogenous polynucleotide comprising a nucleic acid sequenceencoding a polypeptide selected from the group consisting of SEQ ID NOs:474-643, 645-760, 4806-6390, 6394-6398, 6400-7249, 7251-8134, 8136-8163and 8164, thereby increasing the yield, harvest index, growth rate,biomass, vigor, oil content, seed yield, fiber yield, fiber qualityfiber length, photosynthetic capacity, nitrogen use efficiency, and/orabiotic stress tolerance of the plant.

According to an aspect of some embodiments of the present inventionthere is provided a method of producing a crop comprising growing a cropplant transformed with an exogenous polynucleotide comprising a nucleicacid sequence encoding a polypeptide at least 80% homologous (e.g.,identical) to the amino acid sequence selected from the group consistingof SEQ ID NOs: 474-643, 645-679, 681-755, 757-760, 4806-6390, 6395-6396,6401-6895, 6897-7249, 7251-7685, 7687-7693, 7695-7700, 7702-7708,7710-7796, 7798-7816, 7818, 7820-7837, 7839-7840, 7842-7861, 7863-8134,8136-8163 and 8164, wherein the crop plant is derived from plants whichhave been transformed with the exogenous polynucleotide and which havebeen selected for increased yield, increased harvest index, increasedgrowth rate, increased biomass, increased vigor, increased oil content,increased seed yield, increased fiber yield, increased fiber quality,increased fiber length, increased photosynthetic capacity, increasednitrogen use efficiency, and/or increased abiotic stress tolerance ascompared to a wild type plant of the same species which is grown underthe same growth conditions, and the crop plant having the increasedyield, increased harvest index, increased growth rate, increasedbiomass, increased vigor, increased oil content, increased seed yield,increased fiber yield, increased fiber quality, increased fiber length,increased photosynthetic capacity, increased nitrogen use efficiency,and/or increased abiotic stress tolerance, thereby producing the crop.

According to an aspect of some embodiments of the present inventionthere is provided a method of increasing yield, harvest index, growthrate, biomass, vigor, oil content, seed yield, fiber yield, fiberquality, fiber length, photosynthetic capacity, nitrogen use efficiency,and/or abiotic stress tolerance of a plant, comprising expressing withinthe plant an exogenous polynucleotide comprising a nucleic acid sequenceat least 80% identical to SEQ ID NO: 1-170, 172-267, 269-424, 426-473,761-2486, 2489-2494, 2496-4803 or 4804, thereby increasing the yield,harvest index, growth rate, biomass, vigor, oil content, seed yield,fiber yield, fiber quality, fiber length, photosynthetic capacity,nitrogen use efficiency, and/or abiotic stress tolerance of the plant.

According to an aspect of some embodiments of the present inventionthere is provided a method of increasing yield, harvest index, growthrate, biomass, vigor, oil content, seed yield, fiber yield, fiberquality, fiber length, photosynthetic capacity, nitrogen use efficiency,and/or abiotic stress tolerance of a plant, comprising expressing withinthe plant an exogenous polynucleotide comprising the nucleic acidsequence selected from the group consisting of SEQ ID NOs:1-473,761-4804 and 4805, thereby increasing the yield, harvest index, growthrate, biomass, vigor, oil content, seed yield, fiber yield, fiberquality, fiber length, photosynthetic capacity, nitrogen use efficiency,and/or abiotic stress tolerance of the plant.

According to an aspect of some embodiments of the present inventionthere is provided a method of producing a crop comprising growing a cropplant transformed with an exogenous polynucleotide which comprises anucleic acid sequence which is at least 80% identical to the nucleicacid sequence selected from the group consisting of SEQ ID NOs:1-170,172-267, 269-424, 426-473, 761-2486, 2489-2494, 2496-4803 and 4804,wherein the crop plant is derived from plants which have beentransformed with the exogenous polynucleotide and which have beenselected for increased yield, increase harvest index, increased growthrate, increased biomass, increased vigor, increased oil content,increased seed yield, increased fiber yield, increased fiber quality,increased fiber length, increased photosynthetic capacity, increasednitrogen use efficiency, and/or increased abiotic stress tolerance ascompared to a wild type plant of the same species which is grown underthe same growth conditions, and the crop plant having the increasedyield, increase harvest index, increased growth rate, increased biomass,increased vigor, increased oil content, increased seed yield, increasedfiber yield, increased fiber quality, increased fiber length, increasedphotosynthetic capacity, increased nitrogen use efficiency, and/orincreased abiotic stress tolerance, thereby producing the crop.

According to an aspect of some embodiments of the present inventionthere is provided an isolated polynucleotide comprising a nucleic acidsequence encoding a polypeptide which comprises an amino acid sequenceat least 80% homologous to the amino acid sequence set forth in SEQ IDNO:474-643, 645-679, 681-755, 757-760, 4806-6390, 6395-6396, 6401-6895,6897-7249, 7251-7685, 7687-7693, 7695-7700, 7702-7708, 7710-7796,7798-7816, 7818, 7820-7837, 7839-7840, 7842-7861, 7863-8134, 8136-8163or 8164, wherein the amino acid sequence is capable of increasing yield,harvest index, growth rate, biomass, vigor, oil content, seed yield,fiber yield, fiber quality, fiber length, photosynthetic capacity,nitrogen use efficiency, and/or abiotic stress tolerance of a plant.

According to an aspect of some embodiments of the present inventionthere is provided an isolated polynucleotide comprising a nucleic acidsequence encoding a polypeptide which comprises the amino acid sequenceselected from the group consisting of SEQ ID NOs: 474-643, 645-760,4806-6390, 6394-6398, 6400-7249, 7251-8134, 8136-8163 and 8164.

According to an aspect of some embodiments of the present inventionthere is provided an isolated polynucleotide comprising a nucleic acidsequence at least 80% identical to SEQ ID NOs: 1-170, 172-267, 269-424,426-473, 761-2486, 2489-2494, 2496-4803 and 4804, wherein the nucleicacid sequence is capable of increasing yield, harvest index, growthrate, biomass, vigor, oil content, seed yield, fiber yield, fiberquality, fiber length, photosynthetic capacity, nitrogen use efficiency,and/or abiotic stress tolerance of a plant.

According to an aspect of some embodiments of the present inventionthere is provided an isolated polynucleotide comprising the nucleic acidsequence selected from the group consisting of SEQ ID NOs: 1-473,761-4804 and 4805.

According to an aspect of some embodiments of the present inventionthere is provided a nucleic acid construct comprising the isolatedpolynucleotide of some embodiments of the invention, and a promoter fordirecting transcription of the nucleic acid sequence in a host cell.

According to an aspect of some embodiments of the present inventionthere is provided an isolated polypeptide comprising an amino acidsequence at least 80% homologous to SEQ ID NO: 474-643, 645-679,681-755, 757-760, 4806-6390, 6395-6396, 6401-6895, 6897-7249, 7251-7685,7687-7693, 7695-7700, 7702-7708, 7710-7796, 7798-7816, 7818, 7820-7837,7839-7840, 7842-7861, 7863-8134, 8136-8163 or 8164, wherein the aminoacid sequence is capable of increasing yield, harvest index, growthrate, biomass, vigor, oil content, seed yield, fiber yield, fiberquality, fiber length, photosynthetic capacity, nitrogen use efficiency,and/or abiotic stress tolerance of a plant.

According to an aspect of some embodiments of the present inventionthere is provided an isolated polypeptide comprising the amino acidsequence selected from the group consisting of SEQ ID NOs: 474-643,645-760, 4806-6390, 6394-6398, 6400-7249, 7251-8134, 8136-8163 and 8164.

According to an aspect of some embodiments of the present inventionthere is provided a plant cell exogenously expressing the polynucleotideof some embodiments of the invention, or the nucleic acid construct ofsome embodiments of the invention. According to an aspect of someembodiments of the present invention there is provided a plant cellexogenously expressing the polypeptide of some embodiments of theinvention.

According to some embodiments of the invention, the nucleic acidsequence encodes an amino acid sequence selected from the groupconsisting of SEQ ID NOs: 474-643, 645-760, 4806-6390, 6394-6398,6400-7249, 7251-8134, 8136-8163 and 8164.

According to an aspect of some embodiments of the present inventionthere is provided a method of selecting a transformed plant havingincreased yield, harvest index, growth rate, biomass, vigor, oilcontent, seed yield, fiber yield, fiber quality, fiber length,photosynthetic capacity, nitrogen use efficiency, and/or abiotic stresstolerance as compared to a wild type plant of the same species which isgrown under the same growth conditions, the method comprising:

(a) providing plants transformed with an exogenous polynucleotideencoding a polypeptide comprising an amino acid sequence at least 80%homologous to the amino acid sequence selected from the group consistingof SEQ ID NOs:474-643, 645-679, 681-755, 757-760, 4806-6390, 6395-6396,6401-6895, 6897-7249, 7251-7685, 7687-7693, 7695-7700, 7702-7708,7710-7796, 7798-7816, 7818, 7820-7837, 7839-7840, 7842-7861, 7863-8134,8136-8163 and 8164,

(b) selecting from the plants of step (a) a plant having increasedyield, harvest index, growth rate, biomass, vigor, oil content, seedyield, fiber yield, fiber quality, fiber length, photosyntheticcapacity, nitrogen use efficiency, and/or abiotic stress tolerance ascompared to a wild type plant of the same species which is grown underthe same growth conditions,

thereby selecting the plant having the increased yield, harvest index,growth rate, biomass, vigor, oil content, seed yield, fiber yield, fiberquality, fiber length, photosynthetic capacity, nitrogen use efficiency,and/or abiotic stress tolerance as compared to the wild type plant ofthe same species which is grown under the same growth conditions.

According to an aspect of some embodiments of the present inventionthere is provided a method of selecting a transformed plant havingincreased yield, harvest index, growth rate, biomass, vigor, oilcontent, seed yield, fiber yield, fiber quality, fiber length,photosynthetic capacity, nitrogen use efficiency, and/or abiotic stresstolerance as compared to a wild type plant of the same species which isgrown under the same growth conditions, the method comprising:

(a) providing plants transformed with an exogenous polynucleotide atleast 80% identical to the nucleic acid sequence selected from the groupconsisting of SEQ ID NOs:1-170, 172-267, 269-424, 426-473, 761-2486,2489-2494, 2496-4803 and 4804,

(b) selecting from the plants of step (a) a plant having increasedyield, harvest index, growth rate, biomass, vigor, oil content, seedyield, fiber yield, fiber quality, fiber length, photosyntheticcapacity, nitrogen use efficiency, and/or abiotic stress tolerance ascompared to a wild type plant of the same species which is grown underthe same growth conditions, thereby selecting the plant having theincreased yield, harvest index, growth rate, biomass, vigor, oilcontent, seed yield, fiber yield, fiber quality, fiber length,photosynthetic capacity, nitrogen use efficiency, and/or abiotic stresstolerance as compared to the wild type plant of the same species whichis grown under the same growth conditions.

According to some embodiments of the invention, the nucleic acidsequence is selected from the group consisting of SEQ ID NOs: 1-473,761-4804 and 4805.

According to some embodiments of the invention, the polynucleotideconsists of the nucleic acid sequence selected from the group consistingof SEQ ID NOs: 1-473, 761-4804 and 4805.

According to some embodiments of the invention, the nucleic acidsequence encodes the amino acid sequence selected from the groupconsisting of SEQ ID NOs: 474-643, 645-760, 4806-6390, 6394-6398,6400-7249, 7251-8134, 8136-8163 and 8164.

According to some embodiments of the invention, the plant cell formspart of a plant.

According to some embodiments of the invention, the method furthercomprising growing the plant expressing the exogenous polynucleotideunder the abiotic stress.

According to some embodiments of the invention, the abiotic stress isselected from the group consisting of salinity, drought, osmotic stress,water deprivation, flood, etiolation, low temperature, high temperature,heavy metal toxicity, anaerobiosis, nutrient deficiency, nitrogendeficiency, nutrient excess, atmospheric pollution and UV irradiation.

According to some embodiments of the invention, the yield comprises seedyield or oil yield.

According to an aspect of some embodiments of the present inventionthere is provided a transgenic plant comprising the nucleic acidconstruct of some embodiments of the invention or the plant cell of someembodiments of the invention.

According to some embodiments of the invention, the method furthercomprising growing the plant expressing the exogenous polynucleotideunder nitrogen-limiting conditions.

According to some embodiments of the invention, the promoter isheterologous to the isolated polynucleotide and/or to the host cell.

According to an aspect of some embodiments of the present inventionthere is provided a method of growing a crop, the method comprisingseeding seeds and/or planting plantlets of a plant transformed with theisolated polynucleotide of some embodiments of the invention, or withthe nucleic acid construct of some embodiments of the invention, whereinthe plant is derived from plants which have been transformed with theexogenous polynucleotide and which have been selected for at least onetrait selected from the group consisting of: increased nitrogen useefficiency, increased abiotic stress tolerance, increased biomass,increased growth rate, increased vigor, increased yield, increasedharvest index, increased fiber yield, increased fiber quality, increasedfiber length, increased photosynthetic capacity, and increased oilcontent as compared to a non-transformed plant, thereby growing thecrop.

According to some embodiments of the invention, the non-transformedplant is a wild type plant of identical genetic background.

According to some embodiments of the invention, the non-transformedplant is a wild type plant of the same species.

According to some embodiments of the invention, the non-transformedplant is grown under identical growth conditions.

According to some embodiments of the invention, the method furthercomprising selecting a plant having an increased yield, harvest index,growth rate, biomass, vigor, oil content, seed yield, fiber yield, fiberquality, fiber length, photosynthetic capacity, nitrogen use efficiency,and/or abiotic stress tolerance as compared to the wild type plant ofthe same species which is grown under the same growth conditions.

According to some embodiments of the invention, selecting is performedunder non-stress conditions.

According to some embodiments of the invention, selecting is performedunder abiotic stress conditions.

According to some embodiments of the invention, selecting is performedunder nitrogen limiting conditions.

Unless otherwise defined, all technical and/or scientific terms usedherein have the same meaning as commonly understood by one of ordinaryskill in the art to which the invention pertains. Although methods andmaterials similar or equivalent to those described herein can be used inthe practice or testing of embodiments of the invention, exemplarymethods and/or materials are described below. In case of conflict, thepatent specification, including definitions, will control. In addition,the materials, methods, and examples are illustrative only and are notintended to be necessarily limiting.

BRIEF DESCRIPTION OF THE SEVERAL VIEWS OF THE DRAWINGS

Some embodiments of the invention are herein described, by way ofexample only, with reference to the accompanying drawings. With specificreference now to the drawings in detail, it is stressed that theparticulars shown are by way of example and for purposes of illustrativediscussion of embodiments of the invention. In this regard, thedescription taken with the drawings makes apparent to those skilled inthe art how embodiments of the invention may be practiced.

In the drawings:

FIG. 1 is a schematic illustration of the modified pGI binary plasmidcontaining the new At6669 promoter (SEQ ID NO: 8190) and the GUSintron(pQYN 6669) used for expressing the isolated polynucleotide sequences ofthe invention. RB—T-DNA right border, LB—T-DNA left border; MCS—Multiplecloning site; RE—any restriction enzyme; NOS pro=nopaline synthasepromoter; NPT-II=neomycin phosphotransferase gene; NOS ter=nopalinesynthase terminator. Poly-A signal (polyadenylation signal);GUSintron—the GUS reporter gene (coding sequence and intron). Theisolated polynucleotide sequences of the invention were cloned into thevector while replacing the GUSintron reporter gene.

FIG. 2 is a schematic illustration of the modified pG binary plasmidcontaining the new At6669 promoter (SEQ ID NO: 8190) (pQFN or pQFNc)used for expressing the isolated polynucleotide sequences of theinvention. RB—T-DNA right border; LB—T-DNA left border; MCS—Multiplecloning site; RE—any restriction enzyme; NOS pro=nopaline synthasepromoter; NPT-II=neomycin phosphotransferase gene; NOS ter=nopalinesynthase terminator Poly-A signal (polyadenylation signal): The isolatedpolynucleotide sequences of the invention were cloned into the MCS ofthe vector.

FIGS. 3A-3F are images depicting visualization of root development oftransgenic plants exogenously expressing the polynucleotide of someembodiments of the invention when grown in transparent agar plates undernormal (FIGS. 3A-3B), osmotic stress (15% PEG; FIGS. 3C-3D) ornitrogen-limiting (FIGS. 3E-3F) conditions. The different transgeneswere grown in transparent agar plates for 17 days (7 days nursery and 10days after transplanting). The plates were photographed every 3-4 daysstarting at day 1 after transplanting. FIG. 3A—An image of a photographof plants taken following 10 after transplanting days on agar plateswhen grown under normal (standard) conditions. FIG. 3B—An image of rootanalysis of the plants shown in FIG. 3A in which the lengths of theroots measured are represented by arrows. FIG. 3C—An image of aphotograph of plants taken following 10 days after transplanting on agarplates, grown under high osmotic (PEG 15%) conditions. FIG. 3D—An imageof root analysis of the plants shown in FIG. 3C in which the lengths ofthe roots measured are represented by arrows. FIG. 3E—An image of aphotograph of plants taken following 10 days after transplanting on agarplates, grown under low nitrogen conditions. FIG. 3F—An image of rootanalysis of the plants shown in FIG. 3E in which the lengths of theroots measured are represented by arrows.

FIG. 4 is a schematic illustration of the modified pGI binary plasmidcontaining the Root Promoter (pQNa RP) used for expressing the isolatedpolynucleotide sequences of the invention. RB—T-DNA right border,LB—T-DNA left border; NOS pro=nopaline synthase promoter;NPT-II=neomycin phosphotransferase gene: NOS ter=nopaline synthaseterminator; Poly-A signal (polyadenylation signal). The isolatedpolynucleotide sequences according to some embodiments of the inventionwere cloned into the MCS (Multiple cloning site) of the vector.

FIG. 5 is a schematic illustration of the pQYN plasmid.

FIG. 6 is a schematic illustration of the pQFN plasmid.

FIG. 7 is a schematic illustration of the pQFYN plasmid.

FIG. 8 is a schematic illustration of the modified pGI binary plasmid(pQXNc) used for expressing the isolated polynucleotide sequences ofsome embodiments of the invention. RB—T-DNA right border; LB—T-DNA leftborder; NOS pro=nopaline synthase promoter; NPT-II=neomycinphosphotransferase gene; NOS ter=nopaline synthase terminator; RE=anyrestriction enzyme; Poly-A signal (polyadenylation signal); 35S—the 35Spromoter (pqfnc; SEQ ID NO: 8186). The isolated polynucleotide sequencesof some embodiments of the invention were cloned into the MCS (Multiplecloning site) of the vector.

FIGS. 9A-9B are schematic illustrations of the pEBbVNi tDNA (FIG. 9A)and the pEBbNi tDNA (FIG. 9B) plasmids used in the Brachypodiumexperiments, pEBbVNi tDNA (FIG. 9A) was used for expression of theisolated polynucleotide sequences of some embodiments of the inventionin Brachypodium. pEBbNi tDNA (FIG. 9B) was used for transformation intoBrachypodium as a negative control. “RB”=right border; “2LBregion”=2repeats of left border; “35S”=35S promoter (SEQ ID NO:8202); “NOSter”=nopaline synthase terminator; “Bar ORF”—BAR open reading frame(GenBank Accession No. JQ293091.1; SEQ ID NO:8203); The isolatedpolynucleotide sequences of some embodiments of the invention werecloned into the Multiple cloning site of the vector using one or more ofthe indicated restriction enzyme sites.

DESCRIPTION OF SPECIFIC EMBODIMENTS OF THE INVENTION

The present invention, in some embodiments thereof, relates to isolatedpolynucleotides and polypeptides, nucleic acid constructs, transgeniccells and transgenic plants comprising same and methods of generatingand using same, and, more particularly, but not exclusively, to methodsof increasing yield, harvest index, biomass, photosynthetic capacity,growth rate, vigor, oil content, fiber yield, fiber quality abioticstress tolerance, fertilizer use efficiency (e.g., nitrogen useefficiency) and/or water use efficiency of a plant of a plant.

Thus, as shown in the Examples section which follows, the presentinventors have utilized bioinformatics tools to identify polynucleotideswhich enhance yield (e.g., seed yield, oil yield, oil content), growthrate, biomass, harvest index, photosynthetic capacity, vigor and/orabiotic stress tolerance of a plant. Genes which affect thetrait-of-interest were identified based on expression profiles of genesof several Barley, Sorghum, Maize, Brachypodium, Foxtail millet, Soybeanand Tomato ecotypes and accessions in various tissues and growthconditions, homology with genes known to affect the trait-of-interestand using digital expression profile in specific tissues and conditions(SEQ ID NOs: 1-473 (polynucleotides), SEQ ID NOs: 474-760(polypeptides). Table 1. Examples 1 and 3-14 of the Examples sectionwhich follows). Homologous (e.g., orthologous) polypeptides andpolynucleotides having the same function were also identified (SEQ IDNOs: 761-4805 (polynucleotides), SEQ ID NOs: 4806-8165 (polypeptides),Table 2, Example 2 of the Examples section which follows). Selectedgenes were cloned into binary vectors (Table 107, Example 15 of theExamples section which follows), and transformed into agrobacterium(Example 16 of the Examples section which follows) for generation oftransgenic plants (e.g., Arabidopsis and Brachypodium transgenic plants.Examples 17-18 of the Examples section which follows) over-expressingthe polynucleotides and polypeptides of some embodiments of theinvention. Transgenic plants over-expressing the identifiedpolynucleotides were evaluated for the effect of the transgene(exogenous expression of the transgene) under normal or stressconditions in greenhouse seed maturation assays (Examples 19 and 23 ofthe Examples section which follows), in greenhouse until bolting assays(Example 20 of the Examples section which follows), and in greenhouseuntil heading assays (Example 22 of the Examples section which follows).These experiments showed significant increases in dry weight, freshweight, leaf blade area, leaf number, plot coverage, harvest index, seedyield, 1000 seed weight, rosette area, rosette diameter, petiolerelative area TP2, petiole relative area TP3, petiole relative area TP4,as well as in growth rate of leaf number, plot coverage and rosettediameter in transgenic plants over-expressing the isolatedpolynucleotides of some embodiments of the invention as compared tocontrol (native, wild type) under the same growth conditions (Tables108-115). In addition, seedling analyses were performed to evaluate theT1 and T2 generations of transgenic plants over-expressing thepolynucleotides of the invention for increased biomass, root systems forabsorbing nitrogen from soil and photosynthetic capacity as compared tocontrol plants (Example 21 of the Examples section which follows, Tables116-121). These experiments showed significant increases in dry weight,fresh weight, leaf area, roots coverage, roots length, as well as ingrowth rate of leaf area, roots coverage and root length in transgenicplants over-expressing the isolated polynucleotides of some embodimentsof the invention as compared to control (native, wild type) under thesame growth conditions (Tables 116-121). Altogether, these resultssuggest the use of the novel polynucleotides and polypeptides of theinvention for increasing yield (including oil yield, seed yield and oilcontent), harvest index, growth rate, photosynthetic capacity, biomass,vigor, nitrogen use efficiency and/or abiotic stress tolerance of aplant.

Thus, according to an aspect of some embodiments of the invention, thereis provided method of increasing oil content, yield, harvest index,growth rate, biomass, vigor, fiber yield, fiber quality, fiber length,photosynthetic capacity, fertilizer use efficiency (e.g., nitrogen useefficiency) and/or abiotic stress tolerance of a plant, comprisingexpressing within the plant an exogenous polynucleotide comprising anucleic acid sequence encoding a polypeptide at least about 80%, atleast about 81%, at least about 82%, at least about 83%, at least about84%, at least about 85%, at least about 86%, at least about 87%, atleast about 88%, at least about 89%, at least about 90%, at least about91%, at least about 92%, at least about 93%, at least about 94%, atleast about 95%, at least about 96%, at least about 97%, at least about98%, at least about 99%, or more say 100% homologous (e.g., identical)to the amino acid sequence selected from the group consisting of SEQ IDNOs: 474-643, 645-679, 681-755, 757-760, 4806-6390, 6395-6396,6401-6895, 6897-7249, 7251-7685, 7687-7693, 7695-7700, 7702-7708,7710-7796, 7798-7816, 7818, 7820-7837, 7839-7840, 7842-7861, 7863-8134,8136-8163 and 8164, thereby increasing the oil content, yield, harvestindex, growth rate, biomass, vigor, fiber yield, fiber quality, fiberlength, photosynthetic capacity, fertilizer use efficiency (e.g.,nitrogen use efficiency) and/or abiotic stress tolerance of the plant.

As used herein the phrase “plant yield” refers to the amount (e.g., asdetermined by weight or size) or quantity (numbers) of tissues or organsproduced per plant or per growing season. Hence increased yield couldaffect the economic benefit one can obtain from the plant in a certaingrowing area and/or growing time.

It should be noted that a plant yield can be affected by variousparameters including, but not limited to, plant biomass; plant vigor;growth rate; seed yield; seed or grain quantity; seed or grain quality;oil yield; content of oil, starch and/or protein in harvested organs(e.g., seeds or vegetative parts of the plant); number of flowers(florets) per panicle (expressed as a ratio of number of filled seedsover number of primary panicles); harvest index; number of plants grownper area; number and size of harvested organs per plant and per area;number of plants per growing area (density); number of harvested organsin field; total leaf area; carbon assimilation and carbon partitioning(the distribution/allocation of carbon within the plant); resistance toshade; number of harvestable organs (e.g. seeds), seeds per pod, weightper seed; and modified architecture [such as increase stalk diameter,thickness or improvement of physical properties (e.g. elasticity)].

As used herein the phrase “seed yield” refers to the number or weight ofthe seeds per plant, seeds per pod, or per growing area or to the weightof a single seed, or to the oil extracted per seed. Hence, seed yieldcan be affected by seed dimensions (e.g., length, width, perimeter, areaand/or volume), number of (filled) seeds and seed filling rate and byseed oil content. Hence increase seed yield per plant could affect theeconomic benefit one can obtain from the plant in a certain growing areaand/or growing time; and increase seed yield per growing area could beachieved by increasing seed yield per plant, and/or by increasing numberof plants grown on the same given area.

The term “seed” (also referred to as “grain” or “kernel”) as used hereinrefers to a small embryonic plant enclosed in a covering called the seedcoat (usually with some stored food), the product of the ripened ovuleof gymnosperm and angiosperm plants which occurs after fertilization andsome growth within the mother plant.

The phrase “oil content” as used herein refers to the amount of lipidsin a given plant organ, either the seeds (seed oil content) or thevegetative portion of the plant (vegetative oil content) and istypically expressed as percentage of dry weight (10% humidity of seeds)or wet weight (for vegetative portion).

It should be noted that oil content is affected by intrinsic oilproduction of a tissue (e.g., seed, vegetative portion), as well as themass or size of the oil-producing tissue per plant or per growth period.

In one embodiment, increase in oil content of the plant can be achievedby increasing the size/mass of a plant's tissue(s) which comprise oilper growth period. Thus, increased oil content of a plant can beachieved by increasing the yield, growth rate, biomass and vigor of theplant.

As used herein the phrase “plant biomass” refers to the amount (e.g.,measured in grams of air-dry tissue) of a tissue produced from the plantin a growing season, which could also determine or affect the plantyield or the yield per growing area. An increase in plant biomass can bein the whole plant or in parts thereof such as aboveground (harvestable)parts, vegetative biomass, roots and seeds.

As used herein the phrase “growth rate” refers to the increase in plantorgan/tissue size per time (can be measured in cm² per day or cm/day).

As used herein the phrase “photosynthetic capacity” (also known as“A_(max)”) is a measure of the maximum rate at which leaves are able tofix carbon during photosynthesis. It is typically measured as the amountof carbon dioxide that is fixed per square meter per second, for exampleas μmol m⁻² sec⁻¹. Plants are able to increase their photosyntheticcapacity by several modes of action, such as by increasing the totalleaves area (e.g., by increase of leaves area, increase in the number ofleaves, and increase in plant's vigor, e.g., the ability of the plant togrow new leaves along time course) as well as by increasing the abilityof the plant to efficiently execute carbon fixation in the leaves.Hence, the increase in total leaves area can be used as a reliablemeasurement parameter for photosynthetic capacity increment.

As used herein the phrase “plant vigor” refers to the amount (measuredby weight) of tissue produced by the plant in a given time. Henceincreased vigor could determine or affect the plant yield or the yieldper growing time or growing area. In addition, early vigor (seed and/orseedling) results in improved field stand.

Improving early vigor is an important objective of modem rice breedingprograms in both temperate and tropical rice cultivars. Long roots areimportant for proper soil anchorage in water-seeded rice. Where rice issown directly into flooded fields, and where plants must emerge rapidlythrough water, longer shoots are associated with vigour. Wheredrill-seeding is practiced, longer mesocotyls and coleoptiles areimportant for good seedling emergence. The ability to engineer earlyvigor into plants would be of great importance in agriculture. Forexample, poor early vigor has been a limitation to the introduction ofmaize (Zea mays L.) hybrids based on Corn Belt germplasm in the EuropeanAtlantic.

It should be noted that a plant yield can be determined under stress(e.g., abiotic stress, nitrogen-limiting conditions) and/or non-stress(normal) conditions.

As used herein, the phrase “non-stress conditions” refers to the growthconditions (e.g., water, temperature, light-dark cycles, humidity, saltconcentration, fertilizer concentration in soil, nutrient supply such asnitrogen, phosphorous and/or potassium), that do not significantly gobeyond the everyday climatic and other abiotic conditions that plantsmay encounter, and which allow optimal growth, metabolism, reproductionand/or viability of a plant at any stage in its life cycle (e.g., in acrop plant from seed to a mature plant and back to seed again). Personsskilled in the art are aware of normal soil conditions and climaticconditions for a given plant in a given geographic location. It shouldbe noted that while the non-stress conditions may include some mildvariations from the optimal conditions (which vary from one type/speciesof a plant to another), such variations do not cause the plant to ceasegrowing without the capacity to resume growth.

The phrase “abiotic stress” as used herein refers to any adverse effecton metabolism, growth, reproduction and/or viability of a plant.Accordingly, abiotic stress can be induced by suboptimal environmentalgrowth conditions such as, for example, salinity, osmotic stress, waterdeprivation, drought, flooding, freezing, low or high temperature, heavymetal toxicity, anaerobiosis, nutrient deficiency (e.g., nitrogendeficiency or limited nitrogen), atmospheric pollution or UVirradiation. The implications of abiotic stress are discussed in theBackground section.

The phrase “abiotic stress tolerance” as used herein refers to theability of a plant to endure an abiotic stress without suffering asubstantial alteration in metabolism, growth, productivity and/orviability.

Plants are subject to a range of environmental challenges. Several ofthese, including salt stress, general osmotic stress, drought stress andfreezing stress, have the ability to impact whole plant and cellularwater availability. Not surprisingly, then, plant responses to thiscollection of stresses are related. Zhu (0.2002) Ann. Rev. Plant Biol.53: 247-273 et al. note that “most studies on water stress signalinghave focused on salt stress primarily because plant responses to saltand drought are closely related and the mechanisms overlap”. Manyexamples of similar responses and pathways to this set of stresses havebeen documented. For example, the CBF transcription factors have beenshown to condition resistance to salt, freezing and drought (Kasuga etal. (1999) Nature Biotech. 17: 287-291). The Arabidopsis rd29B gene isinduced in response to both salt and dehydration stress, a process thatis mediated largely through an ABA signal transduction process (Uno etal. (2000) Proc. Natl. Acad. Sci. USA 97: 11632-11637), resulting inaltered activity of transcription factors that bind to an upstreamelement within the rd29B promoter. In Mesembryanthemum crystallinum (iceplant), Patharker and Cushman have shown that a calcium-dependentprotein kinase (McCDPK1) is induced by exposure to both drought and saltstresses (Patharker and Cushman (2000) Plant J. 24: 679-691). Thestress-induced kinase was also shown to phosphorylate a transcriptionfactor, presumably altering its activity, although transcript levels ofthe target transcription factor are not altered in response to salt ordrought stress. Similarly, Saijo et al. demonstrated that a ricesalt/drought-induced calmodulin-dependent protein kinase (OsCDPK7)conferred increased salt and drought tolerance to rice whenoverexpressed (Saijo et al. (2000) Plant J. 23: 319-327).

Exposure to dehydration invokes similar survival strategies in plants asdoes freezing stress (see, for example, Yelenosky (1989) Plant Physiol89: 444-451) and drought stress induces freezing tolerance (see, forexample, Siminovitch et al. (1982) Plant Physiol 69: 250-255; and Guy etal. (1992) Planta 188: 265-270). In addition to the induction ofcold-acclimation proteins, strategies that allow plants to survive inlow water conditions may include, for example, reduced surface area, orsurface oil or wax production. In another example increased solutecontent of the plant prevents evaporation and water loss due to heat,drought, salinity, osmoticum, and the like therefore providing a betterplant tolerance to the above stresses.

It will be appreciated that some pathways involved in resistance to onestress (as described above), will also be involved in resistance toother stresses, regulated by the same or homologous genes. Of course,the overall resistance pathways are related, not identical, andtherefore not all genes controlling resistance to one stress willcontrol resistance to the other stresses. Nonetheless, if a geneconditions resistance to one of these stresses, it would be apparent toone skilled in the art to test for resistance to these related stresses.Methods of assessing stress resistance are further provided in theExamples section which follows.

As used herein the phrase “water use efficiency (WUE)” refers to thelevel of organic matter produced per unit of water consumed by theplant, i.e., the dry weight of a plant in relation to the plant's wateruse, e.g., the biomass produced per unit transpiration.

As used herein the phrase “fertilizer use efficiency” refers to themetabolic process(es) which lead to an increase in the plant's yield,biomass, vigor, and growth rate per fertilizer unit applied. Themetabolic process can be the uptake, spread, absorbent, accumulation,relocation (within the plant) and use of one or more of the minerals andorganic moieties absorbed by the plant, such as nitrogen, phosphatesand/or potassium.

As used herein the phrase “fertilizer-limiting conditions” refers togrowth conditions which include a level (e.g., concentration) of afertilizer applied which is below the level needed for normal plantmetabolism, growth, reproduction and/or viability.

As used herein the phrase “nitrogen use efficiency (NUE)” refers to themetabolic process(es) which lead to an increase in the plant's yield,biomass, vigor, and growth rate per nitrogen unit applied. The metabolicprocess can be the uptake, spread, absorbent, accumulation, relocation(within the plant) and use of nitrogen absorbed by the plant.

As used herein the phrase “nitrogen-limiting conditions” refers togrowth conditions which include a level (e.g., concentration) ofnitrogen (e.g., ammonium or nitrate) applied which is below the levelneeded for normal plant metabolism, growth, reproduction and/orviability.

Improved plant NUE and FUE is translated in the field into eitherharvesting similar quantities of yield, while implementing lessfertilizers, or increased yields gained by implementing the same levelsof fertilizers. Thus, improved NUE or FUE has a direct effect on plantyield in the field. Thus, the polynucleotides and polypeptides of someembodiments of the invention positively affect plant yield, seed yield,and plant biomass. In addition, the benefit of improved plant NUE willcertainly improve crop quality and biochemical constituents of the seedsuch as protein yield and oil yield.

It should be noted that improved ABST will confer plants with improvedvigor also under non-stress conditions, resulting in crops havingimproved biomass and/or yield e.g., elongated fibers for the cottonindustry, higher oil content.

The term “fiber” is usually inclusive of thick-walled conducting cellssuch as vessels and tracheids and to fibrillar aggregates of manyindividual fiber cells. Hence, the term “fiber” refers to (a)thick-walled conducting and non-conducting cells of the xylem; (b)fibers of extraxylary origin, including those from phloem, bark, groundtissue, and epidermis; and (c) fibers from stems, leaves, roots, seeds,and flowers or inflorescences (such as those of Sorghum vulgare used inthe manufacture of brushes and brooms).

Example of fiber producing plants, include, but are not limited to,agricultural crops such as cotton, silk cotton tree (Kapok, Ceibapentandra), desert willow, creosote bush, winterfat, balsa, kenaf,roselle, jute, sisal abaca, flax, corn, sugar cane, hemp, ramie, kapok,coir, bamboo, spanish moss and Agave spp. (e.g. sisal).

As used herein the phrase “fiber quality” refers to at least one fiberparameter which is agriculturally desired, or required in the fiberindustry (further described hereinbelow). Examples of such parameters,include but are not limited to, fiber length, fiber strength, fiberfitness, fiber weight per unit length, maturity ratio and uniformity(further described hereinbelow).

Cotton fiber (lint) quality is typically measured according to fiberlength, strength and fineness. Accordingly, the lint quality isconsidered higher when the fiber is longer, stronger and finer.

As used herein the phrase “fiber yield” refers to the amount or quantityof fibers produced from the fiber producing plant.

As used herein the term “increasing” refers to at least about 2%, atleast about 3%, at least about 4%, at least about 5%, at least about10%, at least about 15%, at least about 20%, at least about 30%, atleast about 40%, at least about 50%, at least about 60%, at least about70%, at least about 80%, increase in yield, seed yield, biomass, growthrate, vigor, oil content, seed yield, fiber yield, fiber quality, fiberlength, photosynthetic capacity, abiotic stress tolerance, and/ornitrogen use efficiency of a plant as compared to a native plant or awild type plant [i.e., a plant not modified with the biomolecules(polynucleotide or polypeptides) of the invention, e.g., anon-transformed plant of the same species which is grown under the same(e.g., identical) growth conditions].

The phrase “expressing within the plant an exogenous polynucleotide” asused herein refers to upregulating the expression level of an exogenouspolynucleotide within the plant by introducing the exogenouspolynucleotide into a plant cell or plant and expressing by recombinantmeans, as further described herein below.

As used herein “expressing” refers to expression at the mRNA andoptionally polypeptide level.

As used herein, the phrase “exogenous polynucleotide” refers to aheterologous nucleic acid sequence which may not be naturally expressedwithin the plant (e.g., a nucleic acid sequence from a differentspecies) or which overexpression in the plant is desired. The exogenouspolynucleotide may be introduced into the plant in a stable or transientmanner, so as to produce a ribonucleic acid (RNA) molecule and/or apolypeptide molecule. It should be noted that the exogenouspolynucleotide may comprise a nucleic acid sequence which is identicalor partially homologous to an endogenous nucleic acid sequence of theplant.

The term “endogenous” as used herein refers to any polynucleotide orpolypeptide which is present and/or naturally expressed within a plantor a cell thereof.

According to some embodiments of the invention, the exogenouspolynucleotide of the invention comprises a nucleic acid sequenceencoding a polypeptide having an amino acid sequence at least about 80%,at least about 81%, at least about 82%, at least about 83%, at leastabout 84%, at least about 85%, at least about 86%, at least about 87%,at least about 88%, at least about 89%, at least about 90%, at leastabout 91%, at least about 92%, at least about 93%, at least about 94%,at least about 95%, at least about 96%, at least about 97%, at leastabout 98%, at least about 99%, or more say 100% homologous (e.g.,identical) to the amino acid sequence selected from the group consistingof SEQ ID NOs: 474-643, 645-679, 681-755, 757-760, 4806-6390, 6395-6396,6401-6895, 6897-7249, 7251-7685, 7687-7693, 7695-7700, 7702-7708,7710-7796, 7798-7816, 7818, 7820-7837, 7839-7840, 7842-7861, 7863-8134,8136-8163 and 8164.

Homologous sequences include both orthologous and paralogous sequences.The term “paralogous” relates to gene-duplications within the genome ofa species leading to paralogous genes. The term “orthologous” relates tohomologous genes in different organisms due to ancestral relationship.Thus, orthologs are evolutionary counterparts derived from a singleancestral gene in the last common ancestor of given two species (KooninEV and Galperin MY (Sequence—Evolution—Function: ComputationalApproaches in Comparative Genomics. Boston: Kluwer Academic; 2003.Chapter 2. Evolutionary Concept in Genetics and Genomics. Availablefrom: ncbi (dot) nlm (dot) nih (dot) gov/books/NBK20255) and thereforehave great likelihood of having the same function.

One option to identify orthologues in monocot plant species is byperforming a reciprocal blast search. This may be done by a first blastinvolving blasting the sequence-of-interest against any sequencedatabase, such as the publicly available NCBI database which may befound at: ncbi (dot) nlm (dot) nih (dot) gov. If orthologues in ricewere sought, the sequence-of-interest would be blasted against, forexample, the 28.469 full-length cDNA clones from Oryza sativa Nipponbareavailable at NCBI. The blast results may be filtered. The full-lengthsequences of either the filtered results or the non-filtered results arethen blasted back (second blast) against the sequences of the organismfrom which the sequence-of-interest is derived. The results of the firstand second blasts are then compared. An orthologue is identified whenthe sequence resulting in the highest score (best hit) in the firstblast identifies in the second blast the query sequence (the originalsequence-of-interest) as the best hit. Using the same rational aparalogue (homolog to a gene in the same organism) is found. In case oflarge sequence families, the ClustalW program may be used [ebi (dot) ac(dot) uk/Tools/clustalw2/index (dot) html], followed by aneighbor-joining tree (wikipedia (dot) org/wiki/Neighbor-joining) whichhelps visualizing the clustering.

Homology (e.g., percent homology, sequence identity+sequence similarity)can be determined using any homology comparison software computing apairwise sequence alignment.

As used herein. “sequence identity” or “identity” in the context of twonucleic acid or polypeptide sequences includes reference to the residuesin the two sequences which are the same when aligned. When percentage ofsequence identity is used in reference to proteins it is recognized thatresidue positions which are not identical often differ by conservativeamino acid substitutions, where amino acid residues are substituted forother amino acid residues with similar chemical properties (e.g. chargeor hydrophobicity) and therefore do not change the functional propertiesof the molecule. Where sequences differ in conservative substitutions,the percent sequence identity may be adjusted upwards to correct for theconservative nature of the substitution. Sequences which differ by suchconservative substitutions are considered to have “sequence similarity”or “similarity”. Means for making this adjustment are well-known tothose of skill in the art. Typically this involves scoring aconservative substitution as a partial rather than a full mismatch,thereby increasing the percentage sequence identity. Thus, for example,where an identical amino acid is given a score of 1 and anon-conservative substitution is given a score of zero, a conservativesubstitution is given a score between zero and 1. The scoring ofconservative substitutions is calculated, e.g., according to thealgorithm of Henikoff S and Henikoff J G. [Amino acid substitutionmatrices from protein blocks. Proc. Natl. Acad. Sci. U.S.A. 1992,89(22): 10915-9].

Identity (e.g., percent homology) can be determined using any homologycomparison software, including for example, the BlastN software of theNational Center of Biotechnology Information (NCBI) such as by usingdefault parameters.

According to some embodiments of the invention, the identity is a globalidentity, i.e., an identity over the entire amino acid or nucleic acidsequences of the invention and not over portions thereof.

According to some embodiments of the invention, the term “homology” or“homologous” refers to identity of two or more nucleic acid sequences;or identity of two or more amino acid sequences; or the identity of anamino acid sequence to one or more nucleic acid sequence.

According to some embodiments of the invention, the homology is a globalhomology, i.e., a homology over the entire amino acid or nucleic acidsequences of the invention and not over portions thereof.

The degree of homology or identity between two or more sequences can bedetermined using various known sequence comparison tools. Following is anon-limiting description of such tools which can be used along with someembodiments of the invention.

Pairwise global alignment was defined by S. B. Needleman and C. D.Wunsch. “A general method applicable to the search of similarities inthe amino acid sequence of two proteins” Journal of Molecular Biology,1970, pages 443-53, volume 48).

For example, when starting from a polypeptide sequence and comparing toother polypeptide sequences, the EMBOSS-6.0.1 Needleman-Wunsch algorithm(available fromemboss(dot)sourceforge(dot)net/apps/cvs/emboss/apps/needle(.dot)html)can be used to find the optimum alignment (including gaps) of twosequences along their entire length—a “Global alignment”. Defaultparameters for Needleman-Wunsch algorithm (EMBOSS-6.0.1) include:gapopen=10; gapextend=0.5; datafile=EBLOSUM62; brief=YES.

According to some embodiments of the invention, the parameters used withthe EMBOSS-6.0.1 tool (for protein-protein comparison) include:gapopen=8; gapextend=2; datafile=EBLOSUM62; brief=YES.

According to some embodiments of the invention, the threshold used todetermine homology using the EMBOSS-6.0.1 Needleman-Wunsch algorithm is80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%,94%, 95%, 96%, 97%, 98%, 99%, or 100%.

When starting from a polypeptide sequence and comparing topolynucleotide sequences, the OneModel FramePlus algorithm [Halperin,E., Faigler, S. and Gill-More, R. (1999)—FramePlus: aligning DNA toprotein sequences. Bioinformatics, 15, 867-873) (available frombiocceleration(dot)com/Products(dot)html] can be used with followingdefault parameters: model=frame+_p2n.model mode=local.

According to some embodiments of the invention, the parameters used withthe OneModel FramePlus algorithm are model=frame+_p2n.model,mode=qglobal.

According to some embodiments of the invention, the threshold used todetermine homology using the OneModel FramePlus algorithm is 80%, 81%,82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%,96%, 97%, 98%, 99%, or 100%.

When starting with a polynucleotide sequence and comparing to otherpolynucleotide sequences the EMBOSS-6.0.1 Needleman-Wunsch algorithm(available fromemboss(dot)sourceforge(dot)net/apps/cvs/emboss/apps/needle(dot)html) canbe used with the following default parameters: (EMBOSS-6.0.1)gapopen=10; gapextend=0.5; datafile=EDNAFULL; brief=YES.

According to some embodiments of the invention, the parameters used withthe EMBOSS-6.0.1 Needleman-Wunsch algorithm are gapopen=10;gapextend=0.2; datafile=EDNAFULL; brief=YES.

According to some embodiments of the invention, the threshold used todetermine homology using the EMBOSS-6.0.1 Needleman-Wunsch algorithm forcomparison of polynucleotides with polynucleotides is 80%, 81%, 82%,83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%,97%, 98%, 99%, or 100%.

According to some embodiment, determination of the degree of homologyfurther requires employing the Smith-Waterman algorithm (forprotein-protein comparison or nucleotide-nucleotide comparison).

Default parameters for GenCore 6.0 Smith-Waterman algorithm include:model=sw.model.

According to some embodiments of the invention, the threshold used todetermine homology using the Smith-Waterman algorithm is 80%, 81%, 82%,83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%,97%, 98%, 99%, or 100%.

According to some embodiments of the invention, the global homology isperformed on sequences which are pre-selected by local homology to thepolypeptide or polynucleotide of interest (e.g., 60% identity over 60%of the sequence length), prior to performing the global homology to thepolypeptide or polynucleotide of interest (e.g., 80% global homology onthe entire sequence). For example, homologous sequences are selectedusing the BLAST software with the Blastp and tBlastn algorithms asfilters for the first stage, and the needle (EMBOSS package) or Frame+algorithm alignment for the second stage. Local identity (Blastalignments) is defined with a very permissive cutoff—60% Identity on aspan of 60% of the sequences lengths because it is used only as a filterfor the global alignment stage. In this specific embodiment (when thelocal identity is used), the default filtering of the Blast package isnot utilized (by setting the parameter “-F F”).

In the second stage, homologs are defined based on a global identity ofat least 80% to the core gene polypeptide sequence.

According to some embodiments of the invention, two distinct forms forfinding the optimal global alignment for protein or nucleotide sequencesare used:

1. Between two proteins (following the blastp filter): EMBOSS-6.0.1Needleman-Wunsch algorithm with the following modified parameters:gapopen=8 gapextend=2. The rest of the parameters are unchanged from thedefault options listed here:

-   Standard (Mandatory) Qualifiers:

[-asequence] sequence Sequence filename and optional format, orreference (input USA)

[-bsequence] seqall Sequence(s) filename and optional format, orreference (input USA)

-   -   gapopen float [10.0 for any sequence]. The gap open penalty is        the score taken away when a gap is created. The best value        depends on the choice of comparison matrix. The default value        assumes you are using the EBLOSUM62 matrix for protein        sequences, and the EDNAFULL matrix for nucleotide sequences.        (Floating point number from 1.0 to 100.0)    -   gapextend float [0.5 for any sequence]. The gap extension,        penalty is added to the standard gap penalty for each base or        residue in the gap. This is how long gaps are penalized. Usually        you will expect a few long gaps rather than many short gaps, so        the gap extension penalty should be lower than the gap penalty.        An exception is where one or both sequences are single reads        with possible sequencing errors in which case you would expect        many single base gaps. You can get this result by setting the        gap open penalty to zero (or very low) and using the gap        extension penalty to control gap scoring. (Floating point number        from 0.0 to 10.0) [-outfile] align [*.needle] Output alignment        file name

Additional (Optional) Qualifiers:

-   -   datafile matrix [EBLOSUM62 for protein, EDNAFULL for DNA]. This        is the scoring matrix file used when comparing sequences. By        default it is the file ‘EBLOSUM62’ (for proteins) or the file        ‘EDNAFULL’ (for nucleic sequences). These files are found in the        ‘data’ directory of the EMBOSS installation.

Advanced (Unprompted) Qualifiers:

-   -   [no]brief Boolean [Y] Brief identity and similarity

Associated Qualifiers:

-   -   “-asequence” associated qualifiers    -   sbegin1 integer Start of the sequence to be used    -   send1 integer End of the sequence to be used    -   sreverse1 boolean Reverse (if DNA)    -   sask1 boolean Ask for begin/end/reverse    -   snucleotide1 boolean Sequence is nucleotide    -   sprotein1 boolean Sequence is protein    -   slower1 boolean Make lowercase    -   supper1 boolean Make upper case    -   sformat1 string Input sequence format    -   sdbname1 string Database name    -   sid1 string Entryname    -   ufo1 string UFO features    -   fformat1 string Features format    -   fopenfile1 string Features file name    -   “-bsequence” associated qualifiers    -   sbegin2 integer Start of each sequence to be used    -   send2 integer End of each sequence to be used    -   sreverse2 boolean Reverse (if DNA)    -   sask2 boolean Ask for begin/end/reverse    -   snucleotide2 boolean Sequence is nucleotide    -   sprotein2 boolean Sequence is protein    -   slower2 boolean Make lower case    -   supper2 boolean Make upper case    -   sformat2 string Input sequence format    -   sdbname2 string Database name    -   sid2 string Entryname    -   ufo2 string UFO features    -   fformat2 string Features format    -   fopenfile2 string Features file name    -   “-outfile” associated qualifiers    -   aformat3 string Alignment format    -   aextension3 string File name extension    -   adirectory3 string Output directory    -   aname3 string Base file name    -   awidth3 integer Alignment width    -   aaccshow3 boolean Show accession number in the header    -   adesshow3 boolean Show description in the header    -   ausashow3 boolean Show the full USA in the alignment    -   aglobal3 boolean Show the full sequence in alignment

General Qualifiers:

-   -   auto boolean Turn off prompts    -   stdout boolean Write first file to standard output    -   filter boolean Read first file from standard input, write first        file to standard output    -   options Boolean Prompt for standard and additional values    -   debug Boolean Write debug output to program.dbg    -   verbose Boolean Report some/full command line options    -   help boolean Report command line options. More information on        associated and general qualifiers can be found with -help        -verbose    -   warning boolean Report warnings    -   error boolean Report errors    -   fatal boolean Report fatal errors    -   die Boolean Report dying program messages

2. Between a protein sequence and a nucleotide sequence (following thetblastn filter): GenCore 6.0 OneModel application utilizing theFrame+algorithm with the following parameters: model=frame+_p2n.modelmode=qglobal -q=protein.sequence -db=nucleotide.sequence. The rest ofthe parameters are unchanged from the default options:

Usage:

om -model=<model_fname>[-q=]query [-db=]database [options]model=<model_fname> Specifies the model that you want to run. All modelssupplied by Compugen are located in the directory $CGNROOT/models/.

Valid Command Line Parameters:

dev=<dev_name> Selects the device to be used by the application.

-   -   Valid devices are:        -   Bic—Bioccelerator (valid for SW, XSW, FRAME_N2P, and            FRAME_P2N models)·        -   xlg—BioXUG (valid for all models except XSW).        -   xlp—BioXUP (valid for SW, FRAME+_N2P, and FRAME_P2N models).        -   xlh—BioXL/H (valid for SW, FRAME+_N2P, and FRAME_P2N            models).        -   soft—Software device (for all models).            q=<query> Defines the query set. The query can be a sequence            file or a database reference. You can specify a query by its            name or by accession number. The format is detected            automatically. However, you may specify a format using the            -qfmt parameter. If you do not specify a query, the program            prompts for one. If the query set is a database reference,            an output file is produced for each sequence in the query.            db=<database name> Chooses the database set. The database            set can be a sequence file or a database reference. The            database format is detected automatically. However, you may            specify a format using -dfmt parameter.            qacc Add this parameter to the command line if you specify            query using accession numbers.            dacc Add this parameter to the command line if you specify a            database using accession numbers.            dfmt/-qfmt=<format_type> Chooses the database/query format            type. Possible formats are:    -   fasta—fasta with seq type auto-detected.    -   fastap—fasta protein seq.    -   fastan—fasta nucleic seq.    -   gcg—gcg format, type is auto-detected.    -   gcg9seq—gcg9 format, type is auto-detected.    -   gcg9seqp—gcg9 format protein seq.    -   gcg9seqn—gcg9 format nucleic seq.    -   nbrf—nbrf seq. type is auto-detected.    -   nbrfp—nbrf protein seq.    -   nbrfn—nbrf nucleic seq.    -   embl—embl and swissprot format.    -   genbank—genbank format (nucleic).    -   blast—blast format.    -   nbrf_gcg—nbrf-gcg seq. type is auto-detected.    -   nbrf_gcgp—nbrf-gcg protein seq.    -   nbrf_gcgn—nbrf-gcg nucleic seq.    -   raw—raw ascii sequence, type is auto-detected.    -   rawp—raw ascii protein sequence.    -   rawn—raw ascii nucleic sequence.    -   pir—pir codata format, type is auto-detected.    -   profile—gcg profile (valid only for -qfmt    -   in SW, XSW, FRAME_P2N. and FRAME+_P2N).        out=<out_fname> The name of the output file.        suffix=<name> The output file name suffix.        gapop=<n> Gap open penalty. This parameter is not valid for        FRAME+. For FrameSearch the default is 12.0. For other searches        the default is 10.0.        gapext=<n> Gap extend penalty. This parameter is not valid for        FRAME+. For FrameSearch the default is 4.0. For other models:        the default for protein searches is 0.05, and the default for        nucleic searches is 1.0.        qgapop=<n> The penalty for opening a gap in the query sequence.        The default is 10.0. Valid for XSW.        qgapext=<n> The penalty for extending a gap in the query        sequence. The default is 0.05. Valid for XSW.        start=<n> The position in the query sequence to begin the        search.        end=<n> The position in the query sequence to stop the search.        qtrans Performs a translated search, relevant for a nucleic        query against a protein database. The nucleic query is        translated to six reading frames and a result is given for each        frame.

Valid for SW and XSW.

dtrans Performs a translated search, relevant for a protein queryagainst a DNA database. Each database entry is translated to six readingframes and a result is given for each frame.

Valid for SW and XSW.

Note: “-qtrans” and “-dtrans” options are mutually exclusive.matrix=<matrix_file> Specifies the comparison matrix to be used in thesearch. The matrix must be in the BLAST format. If the matrix file isnot located in $CGNROOT/tables/matrix, specify the full path as thevalue of the -matrix parameter.trans=<transtab_name> Translation table. The default location for thetable is $CGNROOT/tables/trans.onestrand Restricts the search to just the top strand of thequery/database nucleic sequence.list=<n> The maximum size of the output hit list. The default is 50.docalign=<n> The number of documentation lines preceding each alignment.The default is 10.thr_score=<score_name> The score that places limits on the display ofresults. Scores that are smaller than -thr_min value or larger than-thr_max value are not shown. Valid options are: quality.

-   -   zscore.    -   escore.        thr_max=<n> The score upper threshold. Results that are larger        than -thr_max value are not shown.        thr_min=<n> The score lower threshold. Results that are lower        than -thr_min value are not shown.        align=<n> The number of alignments reported in the output file.        noalign Do not display alignment.        Note: “-align” and “-noalign” parameters are mutually exclusive.        outfmt=<format_name> Specifies the output format type. The        default format is PFS. Possible values are:    -   PFS—PFS text format    -   FASTA—FASTA text format    -   BLAST—BLAST text format        nonorm Do not perform score normalization.        norm=<norm_name> Specifies the normalization method. Valid        options are:    -   log—logarithm normalization.    -   std—standard normalization.    -   stat—Pearson statistical method.        Note: “-nonorm” and “-norm” parameters cannot be used together.        Note: Parameters -xgapop, -xgapext, -fgapop, -fgapext, -ygapop,        -ygapext, -delop, and -delext apply only to FRAME+.        xgapop=<n> The penalty for opening a gap when inserting a codon        (triplet). The default is 12.0.        xgapext=<n> The penalty for extending a gap when inserting a        codon (triplet). The default is 4.0.        ygapop=<n> The penalty for opening a gap when deleting an amino        acid. The default is 12.0.        ygapext=<n> The penalty for extending a gap when deleting an        amino acid. The default is 4.0.        fgapop=<n> The penalty for opening a gap when inserting a DNA        base. The default is 6.0.        fgapext=<n> The penalty for extending a gap when inserting a DNA        base. The default is 7.0.        delop=<n> The penalty for opening a gap when deleting a DNA        base. The default is 6.0.        delext=<n> The penalty for extending a gap when deleting a DNA        base. The default is 7.0.        silent No screen output is produced.        host=<host_name> The name of the host on which the server runs.        By default, the application uses the host specified in the file        $CGNROOT/cgnhosts.        wait Do not go to the background when the device is busy. This        option is not relevant for the Parseq or Soft pseudo device.        batch Run the job in the background. When this option is        specified, the file “$CGNROOT/defaults/batch.defaults” is used        for choosing the batch command. If this file does not exist, the        command “at now” is used to run the job.        Note: “-batch” and “-wait” parameters are mutually exclusive.        version Prints the software version number.        help Displays this help message. To get more specific help type:    -   “om -model=<model_fname>-help”.

According to some embodiments the homology is a local homology or alocal identity.

Local alignments tools include, but are not limited to the BlastP.BlastN, BlastX or TBLASTN software of the National Center ofBiotechnology Information (NCBI), FASTA, and the Smith-Watermanalgorithm.

A tblastn search allows the comparison between a protein sequence to thesix-frame translations of a nucleotide database. It can be a veryproductive way of finding homologous protein coding regions inunannotated nucleotide sequences such as expressed sequence tags (ESTs)and draft genome records (HTG), located in the BLAST databases est andhtgs, respectively.

Default parameters for blastp include: Max target sequences: 100;Expected threshold: e⁻⁵; Word size: 3; Max matches in a query range: 0;Scoring parameters: Matrix—BLOSUM62; filters and masking: Filter—lowcomplexity regions.

Local alignments tools, which can be used include, but are not limitedto, the tBLASTX algorithm, which compares the six-frame conceptualtranslation products of a nucleotide query sequence (both strands)against a protein sequence database. Default parameters include: Maxtarget sequences: 100; Expected threshold: 10; Word size: 3; Max matchesin a query range: 0; Scoring parameters: Matrix—BLOSUM62; filters andmasking: Filter—low complexity regions.

According to some embodiments of the invention, the exogenouspolynucleotide of the invention encodes a polypeptide having an aminoacid sequence at least about 80%, at least about 81%, at least about82%, at least about 83%, at least about 84%, at least about 85%, atleast about 86%, at least about 87%, at least about 88%, at least about89%, at least about 90%, at least about 91%, at least about 92%, atleast about 93%, at least about 94%, at least about 95%, at least about96%, at least about 97%, at least about 98%, at least about 99%, or moresay 100% identical to the amino acid sequence selected from the groupconsisting of SEQ ID NOs:474-643, 645-679, 681-755, 757-760, 4806-6390,6395-6396, 6401-6895, 6897-7249, 7251-7685, 7687-7693, 7695-7700,7702-7708, 7710-7796, 7798-7816, 7818, 7820-7837, 7839-7840, 7842-7861,7863-8134, 8136-8163 and 8164.

According to some embodiments of the invention, the exogenouspolynucleotide of the invention encodes a polypeptide having the aminoacid sequence selected from the group consisting of SEQ ID NOs: 474-643,645-760, 4806-6390.6394-6398, 6400-7249, 7251-8134, 8136-8163 and 8164.

According to some embodiments of the invention, the method of increasingyield, harvest index, biomass, growth rate, vigor, oil content, fiberyield, fiber quality, fiber length, photosynthetic capacity, abioticstress tolerance, and/or nitrogen use efficiency of a plant, is effectedby expressing within the plant an exogenous polynucleotide comprising anucleic acid sequence encoding a polypeptide at least at least about80%, at least about 81%, at least about 82%, at least about 83%, atleast about 84%, at least about 85%, at least about 86%, at least about87%, at least about 88%, at least about 89%, at least about 90%, atleast about 91%, at least about 92%, at least about 93%, at least about94%, at least about 95%, at least about 96%, at least about 97%, atleast about 98%, at least about 99%, or more say 100% identical to theamino acid sequence selected from the group consisting of SEQ IDNOs:474-643.645-679, 681-755, 757-760, 4806-6390, 6395-6396, 6401-6895,6897-7249, 7251-7685, 7687-7693, 7695-7700, 7702-7708, 7710-7796,7798-7816, 7818, 7820-7837, 7839-7840, 7842-7861, 7863-8134.8136-8163and 8164, thereby increasing the yield, harvest index, biomass, growthrate, vigor, oil content, fiber yield, fiber quality, fiber length,photosynthetic capacity, abiotic stress tolerance, and/or nitrogen useefficiency of the plant.

According to some embodiments of the invention, the exogenouspolynucleotide encodes a polypeptide consisting of the amino acidsequence set forth by SEQ ID NO:474-643, 645-760, 4806-6390, 6394-6398,6400-7249, 7251-8134, 8136-8163 or 8164.

According to an aspect of some embodiments of the invention, the methodof increasing yield, harvest index, biomass, growth rate, vigor, oilcontent, fiber yield, fiber quality, fiber length, photosyntheticcapacity, abiotic stress tolerance, and/or nitrogen use efficiency of aplant, is effected by expressing within the plant an exogenouspolynucleotide comprising a nucleic acid sequence encoding a polypeptidecomprising an amino acid sequence selected from the group consisting ofSEQ ID NOs:474-643, 645-760, 4806-6390, 6394-6398, 6400-7249, 7251-8134,8136-8163 and 8164, thereby increasing the yield, harvest index,biomass, growth rate, vigor, oil content, fiber yield, fiber quality,fiber length, photosynthetic capacity, abiotic stress tolerance, and/ornitrogen use efficiency of the plant.

According to an aspect of some embodiments of the invention, there isprovided a method of increasing yield, biomass, growth rate, vigor, oilcontent, fiber yield, fiber quality, fiber length, photosyntheticcapacity, abiotic stress tolerance, and/or nitrogen use efficiency of aplant, comprising expressing within the plant an exogenouspolynucleotide comprising a nucleic acid sequence encoding a polypeptideselected from the group consisting of SEQ ID NOs: 474-643, 645-760,4806-6390, 6394-6398, 6400-7249, 7251-8134, 8136-8163 and 8164, therebyincreasing the yield, harvest index, biomass, growth rate, vigor, oilcontent, fiber yield, fiber quality, fiber length, photosyntheticcapacity, abiotic stress tolerance, and/or nitrogen use efficiency ofthe plant.

According to some embodiments of the invention, the exogenouspolynucleotide encodes a polypeptide consisting of the amino acidsequence set forth by SEQ ID NO: 474-643, 645-760.4806-6390, 6394-6398,6400-7249, 7251-8134, 8136-8163 or 8164.

According to some embodiments of the invention the exogenouspolynucleotide comprises a nucleic acid sequence which is at least about80%, at least about 81%, at least about 82%, at least about 83%, atleast about 84%, at least about 85%, at least about 86%, at least about87%, at least about 88%, at least about 89%, at least about 90%, atleast about 91%, at least about 92%, at least about 93%, at least about93%, at least about 94%, at least about 95%, at least about 96%, atleast about 97%, at least about 98%, at least about 99%, e.g., 100%identical to the nucleic acid sequence selected from the groupconsisting of SEQ ID NOs:1-170, 172-267, 269-424, 426-473, 761-2486,2489-2494, 2496-4803 and 4804.

According to an aspect of some embodiments of the invention, there isprovided a method of increasing yield, harvest index, biomass, growthrate, vigor, oil content, fiber yield, fiber quality, fiber length,photosynthetic capacity, abiotic stress tolerance, and/or nitrogen useefficiency of a plant, comprising expressing within the plant anexogenous polynucleotide comprising a nucleic acid sequence at leastabout 80%, at least about 81%, at least about 82%, at least about 83%,at least about 84%, at least about 85%, at least about 86%, at leastabout 87%, at least about 88%, at least about 89%, at least about 90%,at least about 91%, at least about 92%, at least about 93%, at leastabout 93%, at least about 94%, at least about 95%, at least about 96%,at least about 97%, at least about 98%, at least about 99%, e.g., 100%identical to the nucleic acid sequence selected from the groupconsisting of SEQ ID NOs:1-170, 172-267, 269-424.426-473, 761-2486,2489-2494, 2496-4803 and 4804, thereby increasing the yield, harvestindex, biomass, growth rate, vigor, oil content, fiber yield, fiberquality, fiber length, photosynthetic capacity, abiotic stresstolerance, and/or nitrogen use efficiency of the plant.

According to some embodiments of the invention the exogenouspolynucleotide is at least about 80%, at least about 81%, at least about82%, at least about 83%, at least about 84%, at least about 85%, atleast about 86%, at least about 87%, at least about 88%, at least about89%, at least about 90%, at least about 91%, at least about 92%, atleast about 93%, at least about 93%, at least about 94%, at least about95%, at least about 96%, at least about 97%, at least about 98%, atleast about 99%, e.g., 100% identical to the polynucleotide selectedfrom the group consisting of SEQ ID NOs:1-170, 172-267.269-424.426-473,761-2486.2489-2494, 2496-4803 and 4804.

According to some embodiments of the invention the exogenouspolynucleotide is set forth by SEQ ID NO:1-473, 761-4804 or 4805.

According to some embodiments of the invention the method of increasingyield, harvest index, growth rate, biomass, vigor, oil content, seedyield, fiber yield, fiber quality, fiber length, photosyntheticcapacity, nitrogen use efficiency, and/or abiotic stress tolerance of aplant further comprising selecting a plant (from the transformed plants)having an increased yield, harvest index, growth rate, biomass, vigor,oil content, seed yield, fiber yield, fiber quality, fiber length,photosynthetic capacity, nitrogen use efficiency, and/or abiotic stresstolerance as compared to the wild type plant of the same species whichis grown under the same growth conditions.

It should be noted that selecting a transformed plant having anincreased trait as compared to a native (or non-transformed) plant grownunder the same growth conditions can be performed by selecting for thetrait, e.g., validating the ability of the transformed plant to exhibitthe increased trait using well known assays (e.g., seedling analyses,greenhouse assays) as is further described herein below.

According to some embodiments of the invention selecting is performedunder non-stress conditions.

According to some embodiments of the invention selecting is performedunder abiotic stress conditions.

According to some embodiments of the invention selecting is performedunder nitrogen limiting conditions.

According to an aspect of some embodiments of the invention, there isprovided a method of selecting a transformed plant having increasedyield, harvest index, growth rate, biomass, vigor, oil content, seedyield, fiber yield, fiber quality, fiber length, photosyntheticcapacity, nitrogen use efficiency, and/or abiotic stress tolerance ascompared to a wild type plant of the same species which is grown underthe same growth conditions, the method comprising:

(a) providing plants transformed with an exogenous polynucleotideencoding a polypeptide comprising an amino acid sequence at least about80%, at least about 81%, at least about 82%, at least about 83%, atleast about 84%, at least about 85%, at least about 86%, at least about87%, at least about 88%, at least about 89%, at least about 90%, atleast about 91%, at least about 92%, at least about 93%, at least about93%, at least about 94%, at least about 95%, at least about 96%, atleast about 97%, at least about 98%, at least about 99%. e.g., 100%homologous (e.g., having sequence similarity or sequence identity) tothe amino acid sequence selected from the group consisting of SEQ IDNOs: 474-643, 645-679, 681-755, 757-760, 4806-6390, 6395-6396,6401-6895, 6897-7249, 7251-7685, 7687-7693, 7695-7700, 7702-7708,7710-7796, 7798-7816, 7818, 7820-7837, 7839-7840, 7842-7861, 7863-8134,8136-8163 and 8164,

(b) selecting from the plants of step (a) a plant having increasedyield, harvest index, growth rate, biomass, vigor, oil content, seedyield, fiber yield, fiber quality, fiber length, photosyntheticcapacity, nitrogen use efficiency, and/or abiotic stress tolerance(e.g., by selecting the plants for the increased trait),

thereby selecting the plant having increased yield, harvest index,growth rate, biomass, vigor, oil content, seed yield, fiber yield, fiberquality, fiber length, photosynthetic capacity, nitrogen use efficiency,and/or abiotic stress tolerance as compared to the wild type plant ofthe same species which is grown under the same growth conditions.

According to an aspect of some embodiments of the invention, there isprovided a method of selecting a transformed plant having increasedyield, harvest index, growth rate, biomass, vigor, oil content, seedyield, fiber yield, fiber quality, fiber length, photosyntheticcapacity, nitrogen use efficiency, and/or abiotic stress tolerance ascompared to a wild type plant of the same species which is grown underthe same growth conditions, the method comprising:

(a) providing plants transformed with an exogenous polynucleotide atleast about 80%, at least about 81%, at least about 82%, at least about83%, at least about 84%, at least about 85%, at least about 86%, atleast about 87%, at least about 88%, at least about 89%, at least about90%, at least about 91%, at least about 92%, at least about 93%, atleast about 93%, at least about 94%, at least about 95%, at least about96%, at least about 97%, at least about 98%, at least about 99%, e.g.,100% identical to the nucleic acid sequence selected from the groupconsisting of SEQ ID NOs: 1-170, 172-267, 269-424, 426-473, 761-2486,2489-2494, 2496-4803 and 4804,

(b) selecting from the plants of step (a) a plant having increasedyield, harvest index, growth rate, biomass, vigor, oil content, seedyield, fiber yield, fiber quality, fiber length, photosyntheticcapacity, nitrogen use efficiency, and/or abiotic stress tolerance,

thereby selecting the plant having increased yield, harvest index,growth rate, biomass, vigor, oil content, seed yield, fiber yield, fiberquality, fiber length, photosynthetic capacity, nitrogen use efficiency,and/or abiotic stress tolerance as compared to the wild type plant ofthe same species which is grown under the same growth conditions.

As used herein the term “polynucleotide” refers to a single or doublestranded nucleic acid sequence which is isolated and provided in theform of an RNA sequence, a complementary polynucleotide sequence (cDNA),a genomic polynucleotide sequence and/or a composite polynucleotidesequences (e.g., a combination of the above).

The term “isolated” refers to at least partially separated from thenatural environment e.g., from a plant cell.

As used herein the phrase “complementary polynucleotide sequence” refersto a sequence, which results from reverse transcription of messenger RNAusing a reverse transcriptase or any other RNA dependent DNA polymerase.Such a sequence can be subsequently amplified in vivo or in vitro usinga DNA dependent DNA polymerase.

As used herein the phrase “genomic polynucleotide sequence” refers to asequence derived (isolated) from a chromosome and thus it represents acontiguous portion of a chromosome.

As used herein the phrase “composite polynucleotide sequence” refers toa sequence, which is at least partially complementary and at leastpartially genomic. A composite sequence can include some exonalsequences required to encode the polypeptide of the present invention,as well as some intronic sequences interposing therebetween. Theintronic sequences can be of any source, including of other genes, andtypically will include conserved splicing signal sequences. Suchintronic sequences may further include cis acting expression regulatoryelements.

Nucleic acid sequences encoding the polypeptides of the presentinvention may be optimized for expression. Examples of such sequencemodifications include, but are not limited to, an altered G/C content tomore closely approach that typically found in the plant species ofinterest, and the removal of codons atypically found in the plantspecies commonly referred to as codon optimization.

The phrase “codon optimization” refers to the selection of appropriateDNA nucleotides for use within a structural gene or fragment thereofthat approaches codon usage within the plant of interest. Therefore, anoptimized gene or nucleic acid sequence refers to a gene in which thenucleotide sequence of a native or naturally occurring gene has beenmodified in order to utilize statistically-preferred orstatistically-favored codons within the plant. The nucleotide sequencetypically is examined at the DNA level and the coding region optimizedfor expression in the plant species determined using any suitableprocedure, for example as described in Sardana et al. (1996, Plant CellReports 15:677-681). In this method, the standard deviation of codonusage, a measure of codon usage bias, may be calculated by first findingthe squared proportional deviation of usage of each codon of the nativegene relative to that of highly expressed plant genes, followed by acalculation of the average squared deviation. The formula used is: 1SDCU=n=1 N [(Xn−Yn)/Yn] 2/N, where Xn refers to the frequency of usageof codon n in highly expressed plant genes, where Yn to the frequency ofusage of codon n in the gene of interest and N refers to the totalnumber of codons in the gene of interest. A Table of codon usage fromhighly expressed genes of dicotyledonous plants is compiled using thedata of Murray et al. (1989. Nuc Acids Res. 17:477-498).

One method of optimizing the nucleic acid sequence in accordance withthe preferred codon usage for a particular plant cell type is based onthe direct use, without performing any extra statistical calculations,of codon optimization Tables such as those provided on-line at the CodonUsage Database through the NIAS (National Institute of AgrobiologicalSciences) DNA bank in Japan (kazusa (dot) or (dot) jp/codon/). The CodonUsage Database contains codon usage tables for a number of differentspecies, with each codon usage Table having been statisticallydetermined based on the data present in Genbank.

By using the above Tables to determine the most preferred or mostfavored codons for each amino acid in a particular species (for example,rice), a naturally-occurring nucleotide sequence encoding a protein ofinterest can be codon optimized for that particular plant species. Thisis effected by replacing codons that may have a low statisticalincidence in the particular species genome with corresponding codons, inregard to an amino acid, that are statistically more favored. However,one or more less-favored codons may be selected to delete existingrestriction sites, to create new ones at potentially useful junctions(5′ and 3′ ends to add signal peptide or termination cassettes, internalsites that might be used to cut and splice segments together to producea correct full-length sequence), or to eliminate nucleotide sequencesthat may negatively effect mRNA stability or expression.

The naturally-occurring encoding nucleotide sequence may already, inadvance of any modification, contain a number of codons that correspondto a statistically-favored codon in a particular plant species.Therefore, codon optimization of the native nucleotide sequence maycomprise determining which codons, within the native nucleotidesequence, are not statistically-favored with regards to a particularplant, and modifying these codons in accordance with a codon usage tableof the particular plant to produce a codon optimized derivative. Amodified nucleotide sequence may be fully or partially optimized forplant codon usage provided that the protein encoded by the modifiednucleotide sequence is produced at a level higher than the proteinencoded by the corresponding naturally occurring or native gene.Construction of synthetic genes by altering the codon usage is describedin for example PCT Patent Application 93/07278.

According to some embodiments of the invention, the exogenouspolynucleotide is a non-coding RNA.

As used herein the phrase ‘non-coding RNA” refers to an RNA moleculewhich does not encode an amino acid sequence (a polypeptide). Examplesof such non-coding RNA molecules include, but are not limited to, anantisense RNA, a pre-miRNA (precursor of a microRNA), or a precursor ofa Piwi-interacting RNA (piRNA).

Non-limiting examples of non-coding RNA polynucleotides are provided inSEQ ID NOs: 220, 268, 473, 1276, 1461, 1743, 2314, 3002, 3068, 3449,3779, and 4481.

Thus, the invention encompasses nucleic acid sequences describedhereinabove; fragments thereof, sequences hybridizable therewith,sequences homologous thereto, sequences encoding similar polypeptideswith different codon usage, altered sequences characterized bymutations, such as deletion, insertion or substitution of one or morenucleotides, either naturally occurring or man induced, either randomlyor in a targeted fashion.

According to some embodiments of the invention, the exogenouspolynucleotide encodes a polypeptide comprising an amino acid sequenceat least 80%, at least about 81%, at least about 82%, at least about83%, at least about 84%, at least about 85%, at least about 86%, atleast about 87%, at least about 88%, at least about 89%, at least about90%, at least about 91%, at least about 92%, at least about 93%, atleast about 93%, at least about 94%, at least about 95%, at least about96%, at least about 97%, at least about 98%, at least about 99%, e.g.,100% identical to the amino acid sequence of a naturally occurring plantorthologue of the polypeptide selected from the group consisting of SEQID NOs: 474-643, 645-760, 4806-6390, 6394-6398, 6400-6895, 6897-7249,7251-8134.8136-8163 and 8164.

According to some embodiments of the invention, the polypeptidecomprising an amino acid sequence at least 80%, at least about 81%, atleast about 82%, at least about 83%, at least about 84%, at least about85%, at least about 86%, at least about 87%, at least about 88%, atleast about 89%, at least about 90%, at least about 91%, at least about92%, at least about 93%, at least about 93%, at least about 94%, atleast about 95%, at least about 96%, at least about 97%, at least about98%, at least about 99%, e.g., 100% identical to the amino acid sequenceof a naturally occurring plant orthologue of the polypeptide selectedfrom the group consisting of SEQ ID NOs: 474-643, 645-679, 681-755,757-760, 4806-6390, 6395-6396, 6401-6895.6897-7249, 7251-7685,7687-7693, 7695-7700, 7702-7708, 7710-7796, 7798-7816, 7818, 7820-7837,7839-7840, 7842-7861, 7863-8134.8136-8163 and 8164.

The invention provides an isolated polynucleotide comprising a nucleicacid sequence at least about 80%, at least about 81%, at least about82%, at least about 83%, at least about 84%, at least about 85%, atleast about 86%, at least about 87%, at least about 88%, at least about89%, at least about 90%, at least about 91%, at least about 92%, atleast about 93%, at least about 93%, at least about 94%, at least about95%, at least about 96%, at least about 97%, at least about 98%, atleast about 99%. e.g., 100% identical to the polynucleotide selectedfrom the group consisting of SEQ ID NOs:1-170, 172-267, 269-424,426-473, 761-2486, 2489-2494, 2496-4803 and 4804.

According to some embodiments of the invention the nucleic acid sequenceis capable of increasing yield, growth rate, biomass, vigor, oilcontent, seed yield, fiber yield, fiber quality, fiber length,photosynthetic capacity, nitrogen use efficiency, abiotic stresstolerance and/or water use efficiency of a plant.

According to some embodiments of the invention the isolatedpolynucleotide comprising the nucleic acid sequence selected from thegroup consisting of SEQ ID NOs:1-473, 761-4804 and 4805.

According to some embodiments of the invention the isolatedpolynucleotide is set forth by SEQ ID NO:1-473, 761-4804 or 4805.

The invention provides an isolated polynucleotide comprising a nucleicacid sequence encoding a polypeptide which comprises an amino acidsequence at least about 80%, at least about 81%, at least about 82%, atleast about 83%, at least about 84%, at least about 85%, at least about86%, at least about 87%, at least about 88%, at least about 89%, atleast about 90%, at least about 91%, at least about 92%, at least about93%, at least about 93%, at least about 94%, at least about 95%, atleast about 96%, at least about 97%, at least about 98%, at least about99%, or more say 100% homologous to the amino acid sequence selectedfrom the group consisting of SEQ ID NO: 474-643, 645-679, 681-755,757-760, 4806-6390, 6395-6396, 6401-6895, 6897-7249, 7251-7685,7687-7693, 7695-7700, 7702-7708, 7710-7796, 7798-7816, 7818, 7820-7837,7839-7840, 7842-7861, 7863-8134, 8136-8163 or 8164.

According to some embodiments of the invention the amino acid sequenceis capable of increasing yield, growth rate, biomass, vigor, oilcontent, seed yield, fiber yield, fiber quality, fiber length,photosynthetic capacity, nitrogen use efficiency, abiotic stresstolerance and/or water use efficiency of a plant.

The invention provides an isolated polynucleotide comprising a nucleicacid sequence encoding a polypeptide which comprises the amino acidsequence selected from the group consisting of SEQ ID NOs:474-643,645-760, 4806-6390, 6394-6398, 6400-7249, 7251-8134, 8136-8163 and 8164.

According to an aspect of some embodiments of the invention, there isprovided a nucleic acid construct comprising the isolated polynucleotideof the invention, and a promoter for directing transcription of thenucleic acid sequence in a host cell.

The invention provides an isolated polypeptide comprising an amino acidsequence at least about 80%, at least about 81%, at least about 82%, atleast about 83%, at least about 84%, at least about 85%, at least about86%, at least about 87%, at least about 88%, at least about 89%, atleast about 90%, at least about 91%, at least about 92%, at least about93%, at least about 93%, at least about 94%, at least about 95%, atleast about 96%, at least about 97%, at least about 98%, at least about99%, or more say 100% homologous to an amino acid sequence selected fromthe group consisting of SEQ ID NO: 474-643, 645-679, 681-755, 757-760,4806-6390, 6395-6396, 6401-6895, 6897-7249, 7251-7685, 7687-7693,7695-7700, 7702-7708, 7710-7796, 7798-7816, 7818, 7820-7837, 7839-7840,7842-7861, 7863-8134, 8136-8163 or 8164.

According to some embodiments of the invention, the polypeptidecomprising an amino acid sequence selected from the group consisting ofSEQ ID NOs:474-643, 645-760, 4806-6390, 6394-6398, 6400-7249, 7251-8134,8136-8163 and 8164.

According to some embodiments of the invention, the polypeptide is setforth by SEQ ID NO: 474-643, 645-760, 4806-6390, 6394-6398, 6400-7249,7251-8134, 8136-8163 or 8164.

The invention also encompasses fragments of the above describedpolypeptides and polypeptides having mutations, such as deletions,insertions or substitutions of one or more amino acids, either naturallyoccurring or man induced, either randomly or in a targeted fashion.

The term “plant” as used herein encompasses a whole plant, a graftedplant, ancestor(s) and progeny of the plants and plant parts, includingseeds, shoots, stems, roots (including tubers), rootstock, scion, andplant cells, tissues and organs. The plant may be in any form includingsuspension cultures, embryos, meristematic regions, callus tissue,leaves, gametophytes, sporophytes, pollen, and microspores. Plants thatare particularly useful in the methods of the invention include allplants which belong to the superfamily Viridiplantae, in particularmonocotyledonous and dicotyledonous plants including a fodder or foragelegume, ornamental plant, food crop, tree, or shrub selected from thelist comprising Acacia spp., Acer spp., Actinidia spp., Aesculus spp.,Agathis australis, Albizia amara, Alsophila tricolor, Andropogon spp.,Arachis spp, Areca catechu, Astelia fragrans, Astragalus cicer, Baikiaeaplurijuga, Betula spp., Brassica spp., Bruguiera gymnorrhiza, Burkeaafricana, Butea frondosa, Cadaba farinosa, Calliandra spp. Camelliasinensis, Canna indica, Capsicum spp., Cassia spp., Centroema pubescens,Chacoomeles spp., Cinnamomum cassia, Coffea arabica, Colophospermummopane, Coronillia varia, Cotoneaster serotina, Crataegus spp., Cucumisspp., Cupressus spp., Cyathea dealbata, Cydonia oblonga, Cryptomeriajaponica, Cymbopogon spp., Cynthea dealbata, Cydonia oblonga, Dalbergiamonetaria, Davallia divaricata, Desmodium spp., Dicksonia squarosa,Dibeteropogon amplectens, Dioclea spp. Dolichos spp., Dorycnium rectum,Echinochloa pyramidalis, Ehraffia spp., Eleusine coracana, Eragrestisspp., Erythrina spp., Eucalypfus spp., Euclea schimperi, Eulaliavi/losa, Pagopyrum spp., Feijoa sellowlana, Fragaria spp., Flemingiaspp, Freycinetia banksli, Geranium thunbergii, GinAgo biloba, Glycinejavanica, Gliricidia spp. Gossypium hirsutum, Grevillea spp., Guibourtiacoleosperma, Hedysarum spp., Hemaffhia altissima, Heteropogon contoffus,Hordeum vulgare, Hyparrhenia rufa, Hypericum erectum, Hypeffheliadissolute, Indigo incamata, Iris spp., Leptarrhena pyrolifolia,Lespediza spp., Lettuca spp., Leucaena leucocephala, Loudetia simplex,Lotonus bainesli, Lotus spp., Macrotyloma axillare, Malus spp., Manihotesculenta, Medicago saliva, Metasequoia glyptostroboides, Musasapientum, Nicotianum spp., Onobrychis spp., Ornithopus spp., Oryzaspp., Peltophorum africanum, Pennisetum spp., Persea gratissima, Petuniaspp., Phaseolus spp., Phoenix canariensis, Phormium cookianum, Photiniaspp., Picea glauca, Pinus spp., Pisum sativam, Podocarpus totara,Pogonarthria fleckii, Pogonaffuia squarrosa, Populus spp., Prosopiscineraria, Pseudotsuga menziesii, Pterolobium stellatum, Pyrus communis,Quercus spp., Rhaphiolepsis umbellata, Rhopalostylis sapida, Rhusnatalensis, Ribes grossularia, Ribes spp., Robinia pseudoacacia. Rosaspp., Rubus spp., Salix spp., Schyzachyrium sanguineum, Sciadopitysvefficillata, Sequoia sempervirens, Sequoiadendron giganteum, Sorghumbicolor, Spinacia spp., Sporobolus fimbriatus, Stiburus alopecuroides,Stylosanthos humilis, Tadehagi spp, Taxodium distichum, Themedatriandra, Trifolium spp., Triticum spp., Tsuga heterophylla, Vacciniumspp., Vicia spp., Vitis vinifera, Watsonia pyramidata. Zantedeschiaaethiopica, Zea mays, amaranth, artichoke, asparagus, broccoli, Brusselssprouts, cabbage, canola, carrot, cauliflower, celery, collard greens,flax, kale, lentil, oilseed rape, okra, onion, potato, rice, soybean,straw, sugar beet, sugar cane, sunflower, tomato, squash tea, maize,wheat, barley, rye, oat, peanut, pea, lentil and alfalfa, cotton,rapeseed, canola, pepper, sunflower, tobacco, eggplant, eucalyptus, atree, an ornamental plant, a perennial grass and a forage crop.Alternatively algae and other non-Viridiplantae can be used for themethods of the present invention.

According to some embodiments of the invention, the plant used by themethod of the invention is a crop plant such as rice, maize, wheat,barley, peanut, potato, sesame, olive tree, palm oil, banana, soybean,sunflower, canola, sugarcane, alfalfa, millet, leguminosae (bean, pea),flax, lupinus, rapeseed, tobacco, poplar and cotton.

According to some embodiments of the invention the plant is adicotyledonous plant.

According to some embodiments of the invention the plant is amonocotyledonous plant.

According to some embodiments of the invention, there is provided aplant cell exogenously expressing the polynucleotide of some embodimentsof the invention, the nucleic acid construct of some embodiments of theinvention and/or the polypeptide of some embodiments of the invention.

According to some embodiments of the invention, expressing the exogenouspolynucleotide of the invention within the plant is effected bytransforming one or more cells of the plant with the exogenouspolynucleotide, followed by generating a mature plant from thetransformed cells and cultivating the mature plant under conditionssuitable for expressing the exogenous polynucleotide within the matureplant.

According to some embodiments of the invention, the transformation iseffected by introducing to the plant cell a nucleic acid construct whichincludes the exogenous polynucleotide of some embodiments of theinvention and at least one promoter for directing transcription of theexogenous polynucleotide in a host cell (a plant cell). Further detailsof suitable transformation approaches are provided hereinbelow.

As mentioned, the nucleic acid construct according to some embodimentsof the invention comprises a promoter sequence and the isolatedpolynucleotide of some embodiments of the invention.

According to some embodiments of the invention, the isolatedpolynucleotide is operably linked to the promoter sequence.

A coding nucleic acid sequence is “operably linked” to a regulatorysequence (e.g., promoter) if the regulatory sequence is capable ofexerting a regulatory effect on the coding sequence linked thereto.

As used herein, the term “promoter” refers to a region of DNA which liesupstream of the transcriptional initiation site of a gene to which RNApolymerase binds to initiate transcription of RNA. The promoter controlswhere (e.g., which portion of a plant) and/or when (e.g., at which stageor condition in the lifetime of an organism) the gene is expressed.

According to some embodiments of the invention, the promoter isheterologous to the isolated polynucleotide and/or to the host cell.

As used herein the phrase “heterologous promoter” refers to a promoterfrom a different species or from the same species but from a differentgene locus as of the isolated polynucleotide sequence.

According to some embodiments of the invention, the isolatedpolynucleotide is heterologous to the plant cell (e.g., thepolynucleotide is derived from a different plant species when comparedto the plant cell, thus the isolated polynucleotide and the plant cellare not from the same plant species).

Any suitable promoter sequence can be used by the nucleic acid constructof the present invention. Preferably the promoter is a constitutivepromoter, a tissue-specific, or an abiotic stress-inducible promoter.

According to some embodiments of the invention, the promoter is a plantpromoter, which is suitable for expression of the exogenouspolynucleotide in a plant cell.

Suitable promoters for expression in wheat include, but are not limitedto, Wheat SPA promoter (SEQ ID NO: 8166; Albani et al, Plant Cell, 9:171-184, 1997, which is fully incorporated herein by reference), wheatLMW (SEQ ID NO: 8167 (longer LMW promoter), and SEQ ID NO: 8168 (LMWpromoter) and HMW glutenin-1 (SEQ ID NO: 8169 (Wheat HMW glutenin-1longer promoter); and SEQ ID NO: 8170 (Wheat HMW glutenin-1 Promoter);Thomas and Flavell, The Plant Cell 2:1171-1180; Furtado et al., 2009Plant Biotechnology Journal 7:240-253, each of which is fullyincorporated herein by reference), wheat alpha, beta and gamma gliadins[e.g., SEQ ID NO: 8171 (wheat alpha gliadin. B genome, promoter); SEQ IDNO: 8172 (wheat gamma gliadin promoter); EMBO 3:1409-15, 1984, which isfully incorporated herein by reference], wheat TdPR60 [SEQ ID NO:8173(wheat TdPR60 longer promoter) or SEQ ID NO:8174 (wheat TdPR60promoter); Kovalchuk et al., Plant Mol Biol 71:81-98, 2009, which isfully incorporated herein by reference], maize Ub1 Promoter [cultivarNongda 105 (SEQ ID NO:8175) GenBank: DQ141598.1: Taylor et al., PlantCell Rep 1993 12: 491-495, which is fully incorporated herein byreference; and cultivar B73 (SEQ ID NO:8176); Christensen, A H, et al.Plant Mol. Biol. 18 (4), 675-689 (1992), which is fully incorporatedherein by reference]; rice actin 1 (SEQ ID NO:8177; Mc Elroy et al.1990, The Plant Cell, Vol. 2, 163-171, which is fully incorporatedherein by reference), rice GOS2 [SEQ ID NO: 8178 (rice GOS2 longerpromoter) and SEQ ID NO: 8179 (rice GOS2 Promoter); De Pater et al.Plant J. 1992: 2: 837-44, which is fully incorporated herein byreference], arabidopsis Pho1 [SEQ ID NO: 8180 (arabidopsis Pho1Promoter); Hamburger et al., Plant Cell, 2002; 14: 889-902, which isfully incorporated herein by reference], ExpansinB promoters, e.g., riceExpB5 [SEQ ID NO:8181 (rice ExpB5 longer promoter) and SEQ ID NO: 8182(rice ExpB5 promoter)] and Barley ExpB1 [SEQ ID NO: 8183 (barley ExpB1Promoter), Won et al. Mol Cells, 2010; 30:369-76, which is fullyincorporated herein by reference], barley SS2 (sucrose synthase 2) [(SEQID NO: 8184), Guerin and Carbonero, Plant Physiology May 1997 vol. 114no. 1 55-62, which is fully incorporated herein by reference], and ricePG5a [SEQ ID NO:8185, U.S. Pat. No. 7,700,835, Nakase et al., Plant MolBiol. 32:621-30, 1996, each of which is fully incorporated herein byreference].

Suitable constitutive promoters include, for example, CaMV 35S promoter[SEQ ID NO: 8186 (CaMV 35S (QFNC) Promoter); SEQ ID NO: 8187 (PJJ 35Sfrom Brachypodium); SEQ ID NO: 8188 (CaMV 35S (OLD) Promoter) (Odell etal., Nature 313:810-812, 1985)]. Arabidopsis At6669 promoter (SEQ ID NO:8189 (Arabidopsis At6669 (OLD) Promoter); see PCT Publication No.WO04081173A2 or the new At6669 promoter (SEQ ID NO: 8190 (ArabidopsisAt6669 (NEW) Promoter)); maize Ub1 Promoter [cultivar Nongda 105 (SEQ IDNO:8175); GenBank: DQ141598.1; Taylor et al., Plant Cell Rep 1993 12:491-495, which is fully incorporated herein by reference; and cultivarB73 (SEQ ID NO:8176); Christensen. A H, et al. Plant Mol. Biol. 18 (4),675-689 (1992), which is fully incorporated herein by reference]; riceactin 1 (SEQ ID NO: 8177, McElroy et al., Plant Cell 2:163-171, 1990);pEMU (Last et al., Theor. Appl. Genet. 81:581-588, 1991); CaMV 19S(Nilsson et al., Physiol. Plant 100:456-462, 1997); rice GOS2 [SEQ IDNO: 8178 (rice GOS2 longer Promoter) and SEQ ID NO: 8179 (rice GOS2Promoter), de Pater et al, Plant J November; 2(6):837-44, 1992]; RBCSpromoter (SEQ ID NO:8191); Rice cyclophilin (Bucholz et al, Plant MolBiol. 25(5):837-43, 1994); Maize H3 histone (Lepetit et al, Mol. Gen.Genet. 231: 276-285, 1992); Actin 2 (An et al. Plant J. 10(1); 107-121,1996) and Synthetic Super MAS (Ni et al., The Plant Journal 7: 661-76,1995). Other constitutive promoters include those in U.S. Pat. Nos.5,659,026, 5,608,149; 5,608,144; 5,604,121; 5,569,597; 5,466,785;5,399,680; 5,268,463; and 5,608,142.

Suitable tissue-specific promoters include, but not limited to,leaf-specific promoters [e.g., AT5G06690 (Thioredoxin) (high expression,SEQ ID NO: 8192). AT5G61520 (AtSTP3) (low expression, SEQ ID NO: 8193)described in Buttner et al 2000 Plant, Cell and Environment 23, 175-184,or the promoters described in Yamamoto et al., Plant J. 12:255-265,1997; Kwon et al., Plant Physiol. 105:357-67, 1994; Yamamoto et al.,Plant Cell Physiol. 35:773-778, 1994; Gotor et al., Plant J. 3:509-18,1993; Orozco et al., Plant Mol. Biol. 23:1129-1138, 1993; and Matsuokaet al., Proc. Natl. Acad. Sci. USA 90:9586-9590, 1993; as well asArabidopsis STP3 (AT5G61520) promoter (Buttner et al., Plant. Cell andEnvironment 23:175-184, 2000)], seed-preferred promoters [e.g., Napin(originated from Brassica napus which is characterized by a seedspecific promoter activity; Stuitje A. R, et. al. Plant BiotechnologyJournal 1 (4): 301-309; SEQ ID NO: 8194 (Brassica napus NAPIN Promoter)from seed specific genes (Simon, et al., Plant Mol. Biol. 5, 191, 1985;Scofield, et al., J. Biol. Chem. 262: 12202, 1987; Baszczynski, et al.,Plant Mol. Biol. 14: 633, 1990), rice PG5a (SEQ ID NO: 8185; U.S. Pat.No. 7,700,835), early seed development Arabidopsis BAN (AT1G61720) (SEQID NO: 8195. US 2009/0031450 A1), late seed development Arabidopsis ABI3(AT3G24650) (SEQ ID NO: 8196 (Arabidopsis ABI3 (AT3G24650) longerPromoter) or 8197 (Arabidopsis ABI3 (AT3G24650) Promoter)) (Ng et al.,Plant Molecular Biology 54: 25-38, 2004). Brazil Nut albumin (Pearson'et al., Plant Mol. Biol. 18: 235-245, 1992), legumin (Ellis, et al.Plant Mol. Biol. 10: 203-214, 1988). Glutelin (rice) (Takaiwa, et al.,Mol. Gen. Genet. 208: 15-22, 1986; Takaiwa, et al., FEBS Letts, 221:43-47, 1987), Zein (Matzke et al Plant Mol Biol, 143). 323-32 1990),napA (Stalberg, et al, Planta 199: 515-519, 1996). Wheat SPA (SEQ IDNO:8166; Albani et al, Plant Cell, 9: 171-184, 1997), sunflower oleosin(Cummins, et al., Plant Mol. Biol. 19: 873-876, 1992)], endospermspecific promoters [e.g., wheat LMW (SEQ ID NO: 8167 (Wheat LMW LongerPromoter), and SEQ ID NO: 8168 (Wheat LMW Promoter) and HMW glutenin-1[(SEQ ID NO: 8169 (Wheat HMW glutenin-1 longer Promoter)) and SEQ ID NO:8170 (Wheat HMW glutenin-1 Promoter). Thomas and Flavell. The Plant Cell2:1171-1180, 1990; Mol Gen Genet 216:81-90.1989; NAR 17:461-2), wheatalpha, beta and gamma gliadins (SEQ ID NO: 8171 (wheat alpha gliadin (Bgenome) promoter); SEQ ID NO: 8172 (wheat gamma gliadin promoter); EMBO3:1409-15, 1984), Barley ltrl promoter, barley B1, C, D hordein (TheorAppl Gen 98:1253-62, 1999; Plant J 4:343-55, 1993; Mol Gen Genet250:750-60, 1996), Barley DOF (Mena et al. The Plant Journal, 116(1):53-62, 1998). Biz2 (EP99106056.7), Barley SS2 (SEQ ID NO: 8184 (BarleySS2 Promoter); Guerin and Carbonero Plant Physiology 114: 1 55-62,1997), wheat Tarp60 (Kovalchuk et al., Plant Mol Biol 71:81-98, 2009),barley D-hordein (D-Hor) and B-hordein (B-Hor) (Agnelo Furtado, RobertJ. Henry and Alessandro Pellegrineschi (2009)], Synthetic promoter(Vicente-Carbajosa et al., Plant J. 13: 629-640, 1998), rice prolaminNRP33, rice -globulin Glb-1 (Wu et al. Plant Cell Physiology 39(8)885-889, 1998), rice alpha-globulin REB/OHP-1 (Nakase et al. Plant Mol.Biol. 33: 513-S22, 1997), rice ADP-glucose PP (Trans Res 6:157-68,1997), maize ESR gene family (Plant J 12:235-46, 1997), sorgumgamma-kafirin (PMB 32:1029-35, 1996)], embryo specific promoters [e.g.,rice OSH1 (Sato et al, Proc. Natl. Acad. Sci. USA, 93: 8117-8122). KNOX(Postma-Haarsma et al, Plant Mol. Biol. 39:257-71, 1999), rice oleosin(Wu et at, J. Biochem., 123:386, 1998)], and flower-specific promoters[e.g., AtPRP4, chalene synthase (chsA) (Van der Meer, et al., Plant Mol.Biol. 15, 95-109, 1990). LAT52 (Twell et al Mol. Gen Genet. 217:240-245;1989). Arabidopsis apetala-3 (Tilly et al., Development, 125:1647-57,1998). Arabidopsis APETALA 1 (AT1G69120. AP1) (SEQ ID NO: 8198(Arabidopsis (ATG69120) APETALA 1)) (Hempel et al., Development124:3845-3853, 1997)], and root promoters [e.g., the ROOTP promoter [SEQID NO: 8199]; rice ExpB5 (SEQ ID NO:8182 (rice ExpB5 Promoter); or SEQID NO: 8181 (rice ExpB5 longer Promoter)) and barley ExpB1 promoters(SEQ ID NO:8183) (Won et al. Mol. Cells 30: 369-376, 2010); arabidopsisATTPS-CIN (AT3G25820) promoter (SEQ ID NO: 8200; Chen et al., Plant Phys135:1956-66, 2004); arabidopsis Pho1 promoter (SEQ ID NO: 8180,Hamburger et al., Plant Cell, 14: 889-902, 2002), which is also slightlyinduced by stress].

Suitable abiotic stress-inducible promoters include, but not limited to,salt-inducible promoters such as RD29A (Yamaguchi-Shinozalei et al.,Mol. Gen. Genet. 236:331-340, 1993); drought-inducible promoters such asmaize rab17 gene promoter (Pla et. al., Plant Mol. Biol. 21:259-266,1993), maize rab28 gene promoter (Busk et. al., Plant J. 11:1285-1295,1997) and maize lvr2 gene promoter (Pelleschi et. al., Plant Mol. Biol.39:373-380, 1999); heat-inducible promoters such as heat tomatohsp80-promoter from tomato (U.S. Pat. No. 5,187,267).

The nucleic acid construct of some embodiments of the invention canfurther include an appropriate selectable marker and/or an origin ofreplication. According to some embodiments of the invention, the nucleicacid construct utilized is a shuttle vector, which can propagate both inE. coli (wherein the construct comprises an appropriate selectablemarker and origin of replication) and be compatible with propagation incells. The construct according to the present invention can be, forexample, a plasmid, a bacmid, a phagemid, a cosmid, a phage, a virus oran artificial chromosome.

The nucleic acid construct of some embodiments of the invention can beutilized to stably or transiently transform plant cells. In stabletransformation, the exogenous polynucleotide is integrated into theplant genome and as such it represents a stable and inherited trait. Intransient transformation, the exogenous polynucleotide is expressed bythe cell transformed but it is not integrated into the genome and assuch it represents a transient trait.

There are various methods of introducing foreign genes into bothmonocotyledonous and dicotyledonous plants (Potrykus, I., Annu. Rev.Plant. Physiol., Plant. Mol. Biol. (1991) 42:205-225; Shimamoto et al.,Nature (1989) 338:274-276).

The principle methods of causing stable integration of exogenous DNAinto plant genomic DNA include two main approaches:

(i) Agrobacterium-mediated gene transfer: Klee et al. (1987) Annu. Rev.Plant Physiol. 38:467-486; Klee and Rogers in Cell Culture and SomaticCell Genetics of Plants. Vol. 6, Molecular Biology of Plant NuclearGenes, eds. Schell, J., and Vasil, L. K., Academic Publishers. SanDiego, Calif. (1989) p. 2-25; Gatenby, in Plant Biotechnology, eds.Kung. S. and Arntzen. C. J., Butterworth Publishers. Boston, Mass.(1989) p. 93-112.

(ii) Direct DNA uptake: Paszkowski et al., in Cell Culture and SomaticCell Genetics of Plants, Vol. 6. Molecular Biology of Plant NuclearGenes eds. Schell. J., and Vasil. L. K., Academic Publishers. San Diego,Calif. (1989) p. 52-68: including methods for direct uptake of DNA intoprotoplasts, Toriyama, K. et al. (1988) Bio/Technology 6:1072-1074. DNAuptake induced by brief electric shock of plant cells: Zhang et al.Plant Cell Rep. (1988) 7:379-384. Fromm et al. Nature (1986)319:791-793. DNA injection into plant cells or tissues by particlebombardment, Klein et al. Bio/Technology (1988) 6:559-563; McCabe et al.Bio/Technology (1988) 6:923-926; Sanford, Physiol. Plant. (0.1990)79:206-209; by the use of micropipette systems: Neuhaus et al., Theor.Appl. Genet. (1987) 75:30-36; Neuhaus and Spangenberg, Physiol. Plant.(1990) 79:213-217; glass fibers or silicon carbide whiskertransformation of cell cultures, embryos or callus tissue, U.S. Pat. No.5,464,765 or by the direct incubation of DNA with germinating pollen,DeWet et al, in Experimental Manipulation of Ovule Tissue, eds. Chapman,G. P. and Mantell, S. H. and Daniels, W. Longman. London, (1985) p.197-209; and Ohta, Proc. Natl. Acad. Sci. USA (1986) 83:715-719.

The Agrobacterium system includes the use of plasmid vectors thatcontain defined DNA segments that integrate into the plant genomic DNA.Methods of inoculation of the plant tissue vary depending upon the plantspecies and the Agrobacterium delivery system. A widely used approach isthe leaf disc procedure which can be performed with any tissue explantthat provides a good source for initiation of whole plantdifferentiation. See, e.g., Horsch et al, in Plant Molecular BiologyManual A5, Kluwer Academic Publishers. Dordrecht (1988) p. 1-9. Asupplementary approach employs the Agrobacterium delivery system incombination with vacuum infiltration. The Agrobacterium system isespecially viable in the creation of transgenic dicotyledonous plants.

There are various methods of direct DNA transfer into plant cells. Inelectroporation, the protoplasts are briefly exposed to a strongelectric field. In microinjection, the DNA is mechanically injecteddirectly into the cells using very small micropipettes. In microparticlebombardment, the DNA is adsorbed on microprojectiles such as magnesiumsulfate crystals or tungsten particles, and the microprojectiles arephysically accelerated into cells or plant tissues.

Following stable transformation plant propagation is exercised. The mostcommon method of plant propagation is by seed. Regeneration by seedpropagation, however, has the deficiency that due to heterozygositythere is a lack of uniformity in the crop, since seeds are produced byplants according to the genetic variances governed by Mendelian rules.Basically, each seed is genetically different and each will grow withits own specific traits. Therefore, it is preferred that the transformedplant be produced such that the regenerated plant has the identicaltraits and characteristics of the parent transgenic plant. Therefore, itis preferred that the transformed plant be regenerated bymicropropagation which provides a rapid, consistent reproduction of thetransformed plants.

Micropropagation is a process of growing new generation plants from asingle piece of tissue that has been excised from a selected parentplant or cultivar. This process permits the mass reproduction of plantshaving the preferred tissue expressing the fusion protein. The newgeneration plants which are produced are genetically identical to, andhave all of the characteristics of, the original plant. Micropropagationallows mass production of quality plant material in a short period oftime and offers a rapid multiplication of selected cultivars in thepreservation of the characteristics of the original transgenic ortransformed plant. The advantages of cloning plants are the speed ofplant multiplication and the quality and uniformity of plants produced.

Micropropagation is a multi-stage procedure that requires alteration ofculture medium or growth conditions between stages. Thus, themicropropagation process involves four basic stages: Stage one, initialtissue culturing; stage two, tissue culture multiplication; stage three,differentiation and plant formation; and stage four, greenhouseculturing and hardening. During stage one, initial tissue culturing, thetissue culture is established and certified contaminant-free. Duringstage two, the initial tissue culture is multiplied until a sufficientnumber of tissue samples are produced from the seedlings to meetproduction goals. During stage three, the tissue samples grown in stagetwo are divided and grown into individual plantlets. At stage four, thetransformed plantlets are transferred to a greenhouse for hardeningwhere the plants' tolerance to light is gradually increased so that itcan be grown in the natural environment.

According to some embodiments of the invention, the transgenic plantsare generated by transient transformation of leaf cells, meristematiccells or the whole plant.

Transient transformation can be effected by any of the direct DNAtransfer methods described above or by viral infection using modifiedplant viruses.

Viruses that have been shown to be useful for the transformation ofplant hosts include CaMV, Tobacco mosaic virus (TMV), brome mosaic virus(BMV) and Bean Common Mosaic Virus (BV or BCMV). Transformation ofplants using plant viruses is described in U.S. Pat. No. 4,855,237 (beangolden mosaic virus; BGV), EP-A 67,553 (TMV), Japanese PublishedApplication No. 63-14693 (TMV). EPA 194.809 (BV), EPA 278.667 (BV); andGluzman. Y. et al., Communications in Molecular Biology: Viral Vectors.Cold Spring Harbor Laboratory. New York. pp. 172-189 (1988). Pseudovirusparticles for use in expressing foreign DNA in many hosts, includingplants are described in WO 87/06261.

According to some embodiments of the invention, the virus used fortransient transformations is avirulent and thus is incapable of causingsevere symptoms such as reduced growth rate, mosaic, ring spots, leafroll, yellowing, streaking, pox formation, tumor formation and pitting.A suitable avirulent virus may be a naturally occurring avirulent virusor an artificially attenuated virus. Virus attenuation may be effectedby using methods well known in the art including, but not limited to,sub-lethal heating, chemical treatment or by directed mutagenesistechniques such as described, for example, by Kurihara and Watanabe(Molecular Plant Pathology 4:259-269, 2003). Gal-on et al. (1992).Atreya et al. (1992) and Huet et al. (1994).

Suitable virus strains can be obtained from available sources such as,for example, the American Type culture Collection (ATCC) or by isolationfrom infected plants. Isolation of viruses from infected plant tissuescan be effected by techniques well known in the art such as described,for example by Foster and Taylor, Eds. “Plant Virology Protocols: FromVirus Isolation to Transgenic Resistance (Methods in Molecular Biology(Humana Pr), Vol 81)”, Humana Press, 1998. Briefly, tissues of aninfected plant believed to contain a high concentration of a suitablevirus, preferably young leaves and flower petals, are ground in a buffersolution (e.g., phosphate buffer solution) to produce a virus infectedsap which can be used in subsequent inoculations.

Construction of plant RNA viruses for the introduction and expression ofnon-viral exogenous polynucleotide sequences in plants is demonstratedby the above references as well as by Dawson, W. O. et al., Virology(1989) 172:285-292; Takamatsu et al. EMBO J. (0.1987) 6:307-311: Frenchet al. Science (1986) 231:1294-1297; Takamatsu et al. FEBS Letters(1990) 269:73-76; and U.S. Pat. No. 5,316,931.

When the virus is a DNA virus, suitable modifications can be made to thevirus itself. Alternatively, the virus can first be cloned into abacterial plasmid for ease of constructing the desired viral vector withthe foreign DNA. The virus can then be excised from the plasmid. If thevirus is a DNA virus, a bacterial origin of replication can be attachedto the viral DNA, which is then replicated by the bacteria.Transcription and translation of this DNA will produce the coat proteinwhich will encapsidate the viral DNA. If the virus is an RNA virus, thevirus is generally cloned as a cDNA and inserted into a plasmid. Theplasmid is then used to make all of the constructions. The RNA virus isthen produced by transcribing the viral sequence of the plasmid andtranslation of the viral genes to produce the coat protein(s) whichencapsidate the viral RNA.

In one embodiment, a plant viral polynucleotide is provided in which thenative coat protein coding sequence has been deleted from a viralpolynucleotide, a non-native plant viral coat protein coding sequenceand a non-native promoter, preferably the subgenomic promoter of thenon-native coat protein coding sequence, capable of expression in theplant host, packaging of the recombinant plant viral polynucleotide, andensuring a systemic infection of the host by the recombinant plant viralpolynucleotide, has been inserted. Alternatively, the coat protein genemay be inactivated by insertion of the non-native polynucleotidesequence within it, such that a protein is produced. The recombinantplant viral polynucleotide may contain one or more additional non-nativesubgenomic promoters. Each non-native subgenomic promoter is capable oftranscribing or expressing adjacent genes or polynucleotide sequences inthe plant host and incapable of recombination with each other and withnative subgenomic promoters. Non-native (foreign) polynucleotidesequences may be inserted adjacent the native plant viral subgenomicpromoter or the native and a non-native plant viral subgenomic promotersif more than one polynucleotide sequence is included. The non-nativepolynucleotide sequences are transcribed or expressed in the host plantunder control of the subgenomic promoter to produce the desiredproducts.

In a second embodiment, a recombinant plant viral polynucleotide isprovided as in the first embodiment except that the native coat proteincoding sequence is placed adjacent one of the non-native coat proteinsubgenomic promoters instead of a non-native coat protein codingsequence.

In a third embodiment, a recombinant plant viral polynucleotide isprovided in which the native coat protein gene is adjacent itssubgenomic promoter and one or more non-native subgenomic promoters havebeen inserted into the viral polynucleotide. The inserted non-nativesubgenomic promoters are capable of transcribing or expressing adjacentgenes in a plant host and are incapable of recombination with each otherand with native subgenomic promoters. Non-native polynucleotidesequences may be inserted adjacent the non-native subgenomic plant viralpromoters such that the sequences are transcribed or expressed in thehost plant under control of the subgenomic promoters to produce thedesired product.

In a fourth embodiment, a recombinant plant viral polynucleotide isprovided as in the third embodiment except that the native coat proteincoding sequence is replaced by a non-native coat protein codingsequence.

The viral vectors are encapsidated by the coat proteins encoded by therecombinant plant viral polynucleotide to produce a recombinant plantvirus. The recombinant plant viral polynucleotide or recombinant plantvirus is used to infect appropriate host plants. The recombinant plantviral polynucleotide is capable of replication in the host, systemicspread in the host, and transcription or expression of foreign gene(s)(exogenous polynucleotide) in the host to produce the desired protein.

Techniques for inoculation of viruses to plants may be found in Fosterand Taylor, eds. “Plant Virology Protocols: From Virus Isolation toTransgenic Resistance (Methods in Molecular Biology (Humana Pr), Vol81)”. Humana Press, 1998; Maramorosh and Koprowski, eds. “Methods inVirology” 7 vols, Academic Press, New York 1967-1984; Hill, S. A.“Methods in Plant Virology”, Blackwell, Oxford, 1984; Walkey, D. G. A.“Applied Plant Virology”, Wiley, New York, 1985; and Kado and Agrawa,eds. “Principles and Techniques in Plant Virology”, VanNostrand-Reinhold, New York.

In addition to the above, the polynucleotide of the present inventioncan also be introduced into a chloroplast genome thereby enablingchloroplast expression.

A technique for introducing exogenous polynucleotide sequences to thegenome of the chloroplasts is known. This technique involves thefollowing procedures. First, plant cells are chemically treated so as toreduce the number of chloroplasts per cell to about one. Then, theexogenous polynucleotide is introduced via particle bombardment into thecells with the aim of introducing at least one exogenous polynucleotidemolecule into the chloroplasts. The exogenous polynucleotides selectedsuch that it is integratable into the chloroplast's genome viahomologous recombination which is readily effected by enzymes inherentto the chloroplast. To this end, the exogenous polynucleotide includes,in addition to a gene of interest, at least one polynucleotide stretchwhich is derived from the chloroplast's genome. In addition, theexogenous polynucleotide includes a selectable marker, which serves bysequential selection procedures to ascertain that all or substantiallyall of the copies of the chloroplast genomes following such selectionwill include the exogenous polynucleotide. Further details relating tothis technique are found in U.S. Pat. Nos. 4,945,050; and 5,693,507which are incorporated herein by reference. A polypeptide can thus beproduced by the protein expression system of the chloroplast and becomeintegrated into the chloroplast's inner membrane.

According to some embodiments, there is provided a method of improvingyield, harvest index, growth rate, biomass, vigor, oil content, seedyield, fiber yield, fiber quality, fiber length, photosyntheticcapacity, nitrogen use efficiency, water use efficiency and/or abioticstress tolerance of a grafted plant, the method comprising providing ascion that does not transgenically express a polynucleotide encoding apolypeptide at least 80% homologous to the amino acid sequence selectedfrom the group consisting of SEQ ID NOs: 474-643, 645-760, 4806-6390,6394-6398, 6400-7249, 7251-8134, 8136-8163 or 8164 and a plant rootstockthat transgenically expresses a polynucleotide encoding a polypeptide atleast about 80%, at least about 81%, at least about 82%, at least about83%, at least about 84%, at least about 85%, at least about 86%, atleast about 87%, at least about 88%, at least about 89%, at least about90%, at least about 91%, at least about 92%, at least about 93%, atleast about 93%, at least about 94%, at least about 95%, at least about96%, at least about 97%, at least about 98%, at least about 99%, e.g.,100% homologous (or identical) to the amino acid sequence selected fromthe group consisting of SEQ ID NOs: 474-643, 645-679, 681-755, 757-760,4806-6390, 6395-6396, 6401-6895, 6897-7249, 7251-7685, 7687-7693,7695-7700, 7702-7708, 7710-7796, 7798-7816, 7818, 7820-7837, 7839-7840,7842-7861, 7863-8134, 8136-8163 and 8164 (e.g., in a constitutive,tissue specific or inducible. e.g., in an abiotic stress responsivemanner), thereby improving the nitrogen use efficiency, yield, harvestindex, growth rate, biomass, vigor, oil content, seed yield, fiberyield, fiber quality, fiber length, photosynthetic capacity, and/orabiotic stress tolerance of the grafted plant.

In some embodiments, the plant scion is non-transgenic.

Several embodiments relate to a grafted plant exhibiting improvednitrogen use efficiency, yield, harvest index, growth rate, biomass,vigor, oil content, seed yield, fiber yield, fiber quality, fiberlength, photosynthetic capacity, and/or abiotic stress tolerance,comprising a scion that does not transgenically express a polynucleotideencoding a polypeptide at least 80% homologous to the amino acidsequence selected from the group consisting of SEQ ID NOs: 474-643,645-760, 4806-6390, 6394-6398, 6400-7249, 7251-8134, 8136-8163 or 8164,and a plant rootstock that transgenically expresses a polynucleotideencoding a polypeptide at least about 80%, at least about 81%, at leastabout 82%, at least about 83%, at least about 84%, at least about 85%,at least about 86%, at least about 87%, at least about 88%, at leastabout 89%, at least about 90%, at least about 91%, at least about 92%,at least about 93%, at least about 93%, at least about 94%, at leastabout 95%, at least about 96%, at least about 97%, at least about 98%,at least about 99%, e.g., 100% homologous (or identical) to the aminoacid sequence selected from the group consisting of SEQ ID NOs: 474-643,645-679, 681-755, 757-760, 4806-6390, 6395-6396, 6401-6895, 6897-7249,7251-7685, 7687-7693, 7695-7700, 7702-7708, 7710-7796, 7798-7816, 7818,7820-7837, 7839-7840, 7842-7861, 7863-8134, 8136-8163 and 8164.

In some embodiments, the plant root stock transgenically expresses apolynucleotide encoding a polypeptide at least about 80%, at least about81%, at least about 82%, at least about 83%, at least about 84%, atleast about 85%, at least about 86%, at least about 87%, at least about88%, at least about 89%, at least about 90%, at least about 91%, atleast about 92%, at least about 93%, at least about 93%, at least about94%, at least about 95%, at least about 96%, at least about 97%, atleast about 98%, at least about 99%. e.g., 100% homologous (oridentical) to the amino acid sequence selected from the group consistingof SEQ ID NOs: 474-643, 645-679, 681-755, 757-760, 4806-6390, 6395-6396,6401-6895, 6897-7249, 7251-7685, 7687-7693, 7695-7700, 7702-7708,7710-7796, 7798-7816, 7818, 7820-7837, 7839-7840, 7842-7861, 7863-8134,8136-8163 and 8164 in a stress responsive manner.

According to some embodiments of the invention, the plant root stocktransgenically expresses a polynucleotide encoding a polypeptideselected from the group consisting of SEQ ID NOs: 474-643, 645-760,4806-6390, 6394-6398, 6400-7249, 7251-8134, 8136-8163 and 8164.

According to some embodiments of the invention, the plant root stocktransgenically expresses a polynucleotide comprising a nucleic acidsequence at least about 80%, at least about 81%, at least about 82%, atleast about 83%, at least about 84%, at least about 85%, at least about86%, at least about 87%, at least about 88%, at least about 89%, atleast about 90%, at least about 91%, at least about 92%, at least about93%, at least about 93%, at least about 94%, at least about 95%, atleast about 96%, at least about 97%, at least about 98%, at least about99%, e.g., 100% identical to the polynucleotide selected from the groupconsisting of SEQ ID NOs: 1-170, 172-267, 269-424, 426-473.761-2486,2489-2494, 2496-4803 and 4804.

According to some embodiments of the invention, the plant root stocktransgenically expresses a polynucleotide selected from the groupconsisting of SEQ ID NOs: 1-473, 761-4804 and 4805.

Since processes which increase yield, growth rate, biomass, vigor, oilcontent, seed yield, fiber yield, fiber quality, fiber length,photosynthetic capacity, nitrogen use efficiency, water use efficiencyand/or abiotic stress tolerance of a plant can involve multiple genesacting additively or in synergy (see, for example, in Quesda et al.,Plant Physiol. 130:951-063, 2002), the present invention also envisagesexpressing a plurality of exogenous polynucleotides in a single hostplant to thereby achieve superior effect on nitrogen use efficiency,fertilizer use efficiency, oil content, yield, seed yield, fiber yield,fiber quality, fiber length, photosynthetic capacity, growth rate,biomass, vigor and/or abiotic stress tolerance.

Expressing a plurality of exogenous polynucleotides in a single hostplant can be effected by co-introducing multiple nucleic acidconstructs, each including a different exogenous polynucleotide, into asingle plant cell. The transformed cell can then be regenerated into amature plant using the methods described hereinabove.

Alternatively, expressing a plurality of exogenous polynucleotides in asingle host plant can be effected by co-introducing into a singleplant-cell a single nucleic-acid construct including a plurality ofdifferent exogenous polynucleotides. Such a construct can be designedwith a single promoter sequence which can transcribe a polycistronicmessenger RNA including all the different exogenous polynucleotidesequences. To enable co-translation of the different polypeptidesencoded by the polycistronic messenger RNA, the polynucleotide sequencescan be inter-linked via an internal ribosome entry site (IRES) sequencewhich facilitates translation of polynucleotide sequences positioneddownstream of the IRES sequence. In this case, a transcribedpolycistronic RNA molecule encoding the different polypeptides describedabove will be translated from both the capped 5′ end and the twointernal IRES sequences of the polycistronic RNA molecule to therebyproduce in the cell all different polypeptides. Alternatively, theconstruct can include several promoter sequences each linked to adifferent exogenous polynucleotide sequence.

The plant cell transformed with the construct including a plurality ofdifferent exogenous polynucleotides, can be regenerated into a matureplant, using the methods described hereinabove.

Alternatively, expressing a plurality of exogenous polynucleotides in asingle host plant can be effected by introducing different nucleic acidconstructs, including different exogenous polynucleotides, into aplurality of plants. The regenerated transformed plants can then becross-bred and resultant progeny selected for superior abiotic stresstolerance, water use efficiency, fertilizer use efficiency, growth,biomass, yield and/or vigor traits, using conventional plant breedingtechniques.

According to some embodiments of the invention, the method furthercomprising growing the plant expressing the exogenous polynucleotideunder the abiotic stress.

Non-limiting examples of abiotic stress conditions include, salinity,osmotic stress, drought, water deprivation, excess of water (e.g.,flood, waterlogging), etiolation, low temperature (e.g., cold stress),high temperature, heavy metal toxicity, anaerobiosis, nutrientdeficiency (e.g., nitrogen deficiency or nitrogen limitation), nutrientexcess, atmospheric pollution and UV irradiation.

According to some embodiments of the invention, the method furthercomprising growing the plant expressing the exogenous polynucleotideunder fertilizer limiting conditions (e.g., nitrogen-limitingconditions). Non-limiting examples include growing the plant on soilswith low nitrogen content (40-50% Nitrogen of the content present undernormal or optimal conditions), or even under sever nitrogen deficiency(0-10% Nitrogen of the content present under normal or optimalconditions, wherein the normal or optimal conditions include about 6-15mM Nitrogen, e.g., 6-10 mM Nitrogen).

Thus, the invention encompasses plants exogenously expressing thepolynucleotide(s), the nucleic acid constructs and/or polypeptide(s) ofthe invention.

Once expressed within the plant cell or the entire plant, the level ofthe polypeptide encoded by the exogenous polynucleotide can bedetermined by methods well known in the art such as, activity assays.Western blots using antibodies capable of specifically binding thepolypeptide. Enzyme-Linked Immuno Sorbent Assay (ELISA),radio-immuno-assays (RIA), immunohistochemistry, immunocytochemistry,immunofluorescence and the like.

Methods of determining the level in the plant of the RNA transcribedfrom the exogenous polynucleotide are well known in the art and include,for example, Northern blot analysis, reverse transcription polymerasechain reaction (RT-PCR) analysis (including quantitative,semi-quantitative or real-time RT-PCR) and RNA—in situ hybridization.

The sequence information and annotations uncovered by the presentteachings can be harnessed in favor of classical breeding. Thus,sub-sequence data of those polynucleotides described above, can be usedas markers for marker assisted selection (MAS), in which a marker isused for indirect selection of a genetic determinant or determinants ofa trait of interest (e.g., biomass, growth rate, oil content, yield,abiotic stress tolerance, water use efficiency, nitrogen use efficiencyand/or fertilizer use efficiency). Nucleic acid data of the presentteachings (DNA or RNA sequence) may contain or be linked to polymorphicsites or genetic markers on the genome such as restriction fragmentlength polymorphism (RFLP), microsatellites and single nucleotidepolymorphism (SNP), DNA fingerprinting (DFP), amplified fragment lengthpolymorphism (AFLP), expression level polymorphism, polymorphism of theencoded polypeptide and any other polymorphism at the DNA or RNAsequence.

Examples of marker assisted selections include, but are not limited to,selection for a morphological trait (e.g., a gene that affects form,coloration, male sterility or resistance such as the presence or absenceof awn, leaf sheath coloration, height, grain color, aroma of rice);selection for a biochemical trait (e.g., a gene that encodes a proteinthat can be extracted and observed; for example, isozymes and storageproteins); selection for a biological trait (e.g., pathogen races orinsect biotypes based on host pathogen or host parasite interaction canbe used as a marker since the genetic constitution of an organism canaffect its susceptibility to pathogens or parasites).

The polynucleotides and polypeptides described hereinabove can be usedin a wide range of economical plants, in a safe and cost effectivemanner.

Plant lines exogenously expressing the polynucleotide or the polypeptideof the invention are screened to identify those that show the greatestincrease of the desired plant trait.

Thus, according to an additional embodiment of the present invention,there is provided a method of evaluating a trait of a plant, the methodcomprising: (a) expressing in a plant or a portion thereof the nucleicacid construct of some embodiments of the invention; and (b) evaluatinga trait of a plant as compared to a wild type plant of the same type(e.g., a plant not transformed with the claimed biomolecules); therebyevaluating the trait of the plant.

According to an aspect of some embodiments of the invention there isprovided a method of producing a crop comprising growing a crop of aplant expressing an exogenous polynucleotide comprising a nucleic acidsequence encoding a polypeptide at least about 80%, at least about 81%,at least about 82%, at least about 83%, at least about 84%, at leastabout 85%, at least about 86%, at least about 87%, at least about 88%,at least about 89%, at least about 90%, at least about 91%, at leastabout 92%, at least about 93%, at least about 94%, at least about 95%,at least about 96%, at least about 97%, at least about 98%, at leastabout 99%, or more say 100% homologous (e.g., identical) to the aminoacid sequence selected from the group consisting of SEQ ID NOs: 474-643,645-679, 681-755, 757-760, 4806-6390, 6395-6396, 6401-6895, 6897-7249,7251-7685, 7687-7693, 7695-7700, 7702-7708, 7710-7796, 7798-7816, 7818,7820-7837, 7839-7840, 7842-7861, 7863-8134, 8136-8163 and 8164, whereinthe plant is derived from a plant (parent plant) that has beentransformed to express the exogenous polynucleotide and that has beenselected for increased abiotic stress tolerance, increased water useefficiency, increased growth rate, increased vigor, increased biomass,increased oil content, increased yield, increased harvest index,increased seed yield, increased fiber yield, increased fiber quality,increased fiber length, increased photosynthetic capacity, and/orincreased fertilizer use efficiency (e.g., increased nitrogen useefficiency) as compared to a control plant, thereby producing the crop.

According to an aspect of some embodiments of the present inventionthere is provided a method of producing a crop comprising growing a cropplant transformed with an exogenous polynucleotide encoding apolypeptide at least 80%, at least about 81%, at least about 82%, atleast about 83%, at least about 84%, at least about 85%, at least about86%, at least about 87%, at least about 88%, at least about 89%, atleast about 90%, at least about 91%, at least about 92%, at least about93%, at least about 94%, at least about 95%, at least about 96%, atleast about 97%, at least about 98%, at least about 99%, or more say100% homologous (e.g., identical) to the amino acid sequence selectedfrom the group consisting of SEQ ID NOs: 474-643, 645-679, 681-755,757-760, 4806-6390, 6395-6396, 6401-6895, 6897-7249, 7251-7685,7687-7693, 7695-7700, 7702-7708, 7710-7796, 7798-7816, 7818, 7820-7837,7839-7840, 7842-7861, 7863-8134, 8136-8163 and 8164, wherein the cropplant is derived from plants which have been transformed with theexogenous polynucleotide and which have been selected for increasedabiotic stress tolerance, increased water use efficiency, increasedgrowth rate, increased vigor, increased biomass, increased oil content,increased yield, increased harvest index, increased seed yield,increased fiber yield, increased fiber quality, increased fiber length,increased photosynthetic capacity, and/or increased fertilizer useefficiency (e.g., increased nitrogen use efficiency) as compared to awild type plant of the same species which is grown under the same growthconditions, and the crop plant having the increased abiotic stresstolerance, increased water use efficiency, increased growth rate,increased vigor, increased biomass, increased oil content, increasedyield, increased harvest index, increased seed yield, increased fiberyield, increased fiber quality, increased fiber length, increasedphotosynthetic capacity, and/or increased fertilizer use efficiency(e.g., increased nitrogen use efficiency), thereby producing the crop.

According to some embodiments of the invention the polypeptide isselected from the group consisting of SEQ ID NOs: 474-643, 645-760,4806-6390, 6394-6398, 6400-7249, 7251-8134, 8136-8163 and 8164.

According to an aspect of some embodiments of the invention there isprovided a method of producing a crop comprising growing a crop of aplant expressing an exogenous polynucleotide which comprises a nucleicacid sequence which is at least about 80%, at least about 81%, at leastabout 82%, at least about 83%, at least about 84%, at least about 85%,at least about 86%, at least about 87%, at least about 88%, at leastabout 89%, at least about 90%, at least about 91%, at least about 92%,at least about 93%, at least about 93%, at least about 94%, at leastabout 95%, at least about 96%, at least about 97%, at least about 98%,at least about 99%, e.g., 100% identical to the nucleic acid sequenceselected from the group consisting of SEQ ID NOs:1-170, 172-267,269-424, 426-473, 761-2486, 2489-2494, 24964803 and 4804, wherein theplant is derived from a plant selected for increased abiotic stresstolerance, increased water use efficiency, increased growth rate,increased vigor, increased biomass, increased oil content, increasedyield, increased harvest index, increased seed yield, increased fiberyield, increased fiber quality, increased fiber length, increasedphotosynthetic capacity, and/or increased fertilizer use efficiency(e.g., increased nitrogen use efficiency) as compared to a controlplant, thereby producing the crop.

According to an aspect of some embodiments of the present inventionthere is provided a method of producing a crop comprising growing a cropplant transformed with an exogenous polynucleotide at least 80%, atleast about 81%, at least about 82%, at least about 83%, at least about84%, at least about 85%, at least about 86%, at least about 87%, atleast about 88%, at least about 89%, at least about 90%, at least about91%, at least about 92%, at least about 93%, at least about 94%, atleast about 95%, at least about 96%, at least about 97%, at least about98%, at least about 99%, or more say 100% identical to the nucleic acidsequence selected from the group consisting of SEQ ID NOs: 1-170,172-267, 269-424, 426-473, 761-2486, 2489-2494, 2496-4803 and 4804,wherein the crop plant is derived from plants which have beentransformed with the exogenous polynucleotide and which have beenselected for increased abiotic stress tolerance, increased water useefficiency, increased growth rate, increased vigor, increased biomass,increased oil content, increased yield, increased harvest index,increased seed yield, increased fiber yield, increased fiber quality,increased fiber length, increased photosynthetic capacity, and/orincreased fertilizer use efficiency (e.g., increased nitrogen useefficiency) as compared to a wild type plant of the same species whichis grown under the same growth conditions, and the crop plant having theincreased abiotic stress tolerance, increased water use efficiency,increased growth rate, increased vigor, increased biomass, increased oilcontent, increased yield, increased harvest index, increased seed yield,increased fiber yield, increased fiber quality, increased fiber length,increased photosynthetic capacity, and/or increased fertilizer useefficiency (e.g., increased nitrogen use efficiency), thereby producingthe crop.

According to some embodiments of the invention the exogenouspolynucleotide is selected from the group consisting of SEQ ID NOs:1-473, 761-4804 and 4805.

According to an aspect of some embodiments of the invention there isprovided a method of growing a crop comprising seeding seeds and/orplanting plantlets of a plant transformed with the exogenouspolynucleotide of the invention, e.g., the polynucleotide which encodesthe polypeptide of some embodiments of the invention, wherein the plantis derived from plants which have been transformed with the exogenouspolynucleotide and which have been selected for at least one traitselected from the group consisting of increased abiotic stresstolerance, increased water use efficiency, increased growth rate,increased vigor, increased biomass, increased oil content, increasedyield, increased harvest index, increased seed yield, increased fiberyield, increased fiber quality, increased fiber length, increasedphotosynthetic capacity, and/or increased fertilizer use efficiency(e.g., increased nitrogen use efficiency) as compared to anon-transformed plant.

According to some embodiments of the invention the method of growing acrop comprising seeding seeds and/or planting plantlets of a planttransformed with an exogenous polynucleotide comprising a nucleic acidsequence encoding a polypeptide at least about 80%, at least about 81%,at least about 82%, at least about 83%, at least about 84%, at leastabout 85%, at least about 86%, at least about 87%, at least about 88%,at least about 89%, at least about 90%, at least about 91%, at leastabout 92%, at least about 93%, at least about 93%, at least about 94%,at least about 95%, at least about 96%, at least about 97%, at leastabout 98%, at least about 99%, e.g., 100% identical to SEQ ID NO:474-643, 645-679, 681-755, 757-760, 4806-6390, 6395-6396, 6401-6895,6897-7249, 7251-7685, 7687-7693, 7695-7700, 7702-7708, 7710-7796,7798-7816, 7818, 7820-7837, 7839-7840, 7842-7861, 7863-8134, 8136-8163or 8164, wherein the plant is derived from plants which have beentransformed with the exogenous polynucleotide and which have beenselected for at least one trait selected from the group consisting ofincreased abiotic stress tolerance, increased water use efficiency,increased growth rate, increased vigor, increased biomass, increased oilcontent, increased yield, increased harvest index, increased seed yield,increased fiber yield, increased fiber quality, increased fiber length,increased photosynthetic capacity, and/or increased fertilizer useefficiency (e.g., increased nitrogen use efficiency) as compared to anon-transformed plant, thereby growing the crop.

According to some embodiments of the invention the polypeptide isselected from the group consisting of SEQ ID NOs: 474-643, 645-760,4806-6390, 6394-6398, 6400-7249, 7251-8134, 8136-8163 and 8164.

According to some embodiments of the invention the method of growing acrop comprising seeding seeds and/or planting plantlets of a planttransformed with an exogenous polynucleotide comprising the nucleic acidsequence at least about 80%, at least about 81%, at least about 82%, atleast about 83%, at least about 84%, at least about 85%, at least about86%, at least about 87%, at least about 88%, at least about 89%, atleast about 90%, at least about 91%, at least about 92%, at least about93%, at least about 93%, at least about 94%, at least about 95%, atleast about 96%, at least about 97%, at least about 98%, at least about99%. e.g., 100% identical to SEQ ID NO: 1-170, 172-267, 269-424,426-473, 761-2486, 2489-2494, 2496-4803 or 4804, wherein the plant isderived from plants which have been transformed with the exogenouspolynucleotide and which have been selected for at least one traitselected from the group consisting of increased abiotic stresstolerance, increased water use efficiency, increased growth rate,increased vigor, increased biomass, increased oil content, increasedyield, increased harvest index, increased seed yield, increased fiberyield, increased fiber quality, increased fiber length, increasedphotosynthetic capacity, and/or increased fertilizer use efficiency(e.g., increased nitrogen use efficiency) as compared to anon-transformed plant, thereby growing the crop.

According to some embodiments of the invention the exogenouspolynucleotide is selected from the group consisting of SEQ ID NOs:1-473, 761-4804 and 4805.

The effect of the transgene (the exogenous polynucleotide encoding thepolypeptide) on abiotic stress tolerance can be determined using knownmethods such as detailed below and in the Examples section whichfollows.

Abiotic stress tolerance—Transformed (i.e., expressing the transgene)and non-transformed (wild type) plants are exposed to an abiotic stresscondition, such as water deprivation, suboptimal temperature (lowtemperature, high temperature), nutrient deficiency, nutrient excess, asalt stress condition, osmotic stress, heavy metal toxicity,anaerobiosis, atmospheric pollution and UV irradiation.

Salinity tolerance assay—Transgenic plants with tolerance to high saltconcentrations are expected to exhibit better germination, seedlingvigor or growth in high salt. Salt stress can be effected in many wayssuch as, for example, by irrigating the plants with a hyperosmoticsolution, by cultivating the plants hydroponically in a hyperosmoticgrowth solution (e.g., Hoagland solution), or by culturing the plants ina hyperosmotic growth medium [e.g., 50% Murashige-Skoog medium (MSmedium)]. Since different plants vary considerably in their tolerance tosalinity, the salt concentration in the irrigation water, growthsolution, or growth medium can be adjusted according to the specificcharacteristics of the specific plant cultivar or variety, so as toinflict a mild or moderate effect on the physiology and/or morphology ofthe plants (for guidelines as to appropriate concentration see,Bernstein and Kafkafi, Root Growth Under Salinity Stress In: PlantRoots, The Hidden Half 3rd ed. Waisel Y, Eshel A and Kafkafi U,(editors) Marcel Dekker Inc., New York, 2002, and reference therein).

For example, a salinity tolerance test can be performed by irrigatingplants at different developmental stages with increasing concentrationsof sodium chloride (for example 50 mM, 100 mM, 200 mM, 400 mM NaCl)applied from the bottom and from above to ensure even dispersal of salt.Following exposure to the stress condition the plants are frequentlymonitored until substantial physiological and/or morphological effectsappear in wild type plants. Thus, the external phenotypic appearance,degree of wilting and overall success to reach maturity and yieldprogeny are compared between control and transgenic plants.

Quantitative parameters of tolerance measured include, but are notlimited to, the average wet and dry weight, growth rate, leaf size, leafcoverage (overall leaf area), the weight of the seeds yielded, theaverage seed size and the number of seeds produced per plant.Transformed plants not exhibiting substantial physiological and/ormorphological effects, or exhibiting higher biomass than wild-typeplants, are identified as abiotic stress tolerant plants.

Osmotic tolerance test—Osmotic stress assays (including sodium chlorideand mannitol assays) are conducted to determine if an osmotic stressphenotype was sodium chloride-specific or if it was a general osmoticstress related phenotype. Plants which are tolerant to osmotic stressmay have more tolerance to drought and/or freezing. For salt and osmoticstress germination experiments, the medium is supplemented for examplewith 50 mM, 100 mM, 200 mM NaCl or 100 mM, 200 mM NaCl, 400 mM mannitol.

Drought tolerance assay/Osmoticum assay—Tolerance to drought isperformed to identify the genes conferring better plant survival afteracute water deprivation. To analyze whether the transgenic plants aremore tolerant to drought, an osmotic stress produced by the non-ionicosmolyte sorbitol in the medium can be performed. Control and transgenicplants are germinated and grown in plant-agar plates for 4 days, afterwhich they are transferred to plates containing 500 mM sorbitol. Thetreatment causes growth retardation, then both control and transgenicplants are compared, by measuring plant weight (wet and dry), yield, andby growth rates measured as time to flowering.

Conversely, soil-based drought screens are performed with plantsoverexpressing the polynucleotides detailed above. Seeds from controlArabidopsis plants, or other transgenic plants overexpressing thepolypeptide of the invention are germinated and transferred to pots.Drought stress is obtained after irrigation is ceased accompanied byplacing the pots on absorbent paper to enhance the soil-drying rate.Transgenic and control plants are compared to each other when themajority of the control plants develop severe wilting. Plants arere-watered after obtaining a significant fraction of the control plantsdisplaying a severe wilting. Plants are ranked comparing to controls foreach of two criteria: tolerance to the drought conditions and recovery(survival) following re-watering.

Cold stress tolerance—To analyze cold stress, mature (25 day old) plantsare transferred to 4° C. chambers for 1 or 2 weeks, with constitutivelight. Later on plants are moved back to greenhouse. Two weeks laterdamages from chilling period, resulting in growth retardation and otherphenotypes, are compared between both control and transgenic plants, bymeasuring plant weight (wet and dry), and by comparing growth ratesmeasured as time to flowering, plant size, yield, and the like.

Heat stress tolerance—Heat stress tolerance is achieved by exposing theplants to temperatures above 34° C. for a certain period. Planttolerance is examined after transferring the plants back to 22° C. forrecovery and evaluation after 5 days relative to internal controls(non-transgenic plants) or plants not exposed to neither cold or heatstress.

Water use efficiency—can be determined as the biomass produced per unittranspiration. To analyze WUE, leaf relative water content can bemeasured in control and transgenic plants. Fresh weight (FW) isimmediately recorded; then leaves are soaked for 8 hours in distilledwater at room temperature in the dark, and the turgid weight (TW) isrecorded. Total dry weight (DW) is recorded after drying the leaves at60° C. to a constant weight. Relative water content (RWC) is calculatedaccording to the following Formula I:

RWC=[(FW−DW)/(TW−DW)]×100  Formula I

Fertilizer use efficiency—To analyze whether the transgenic plants aremore responsive to fertilizers, plants are grown in agar plates or potswith a limited amount of fertilizer, as described, for example, inYanagisawa et al (Proc Natl Acad Sci USA. 2004; 101:7833-8). The plantsare analyzed for their overall size, time to flowering, yield, proteincontent of shoot and/or grain. The parameters checked are the overallsize of the mature plant, its wet and dry weight, the weight of theseeds yielded, the average seed size and the number of seeds producedper plant. Other parameters that may be tested are: the chlorophyllcontent of leaves (as nitrogen plant status and the degree of leafverdure is highly correlated), amino acid and the total protein contentof the seeds or other plant parts such as leaves or shoots, oil content,etc. Similarly, instead of providing nitrogen at limiting amounts,phosphate or potassium can be added at increasing concentrations. Again,the same parameters measured are the same as listed above. In this way,nitrogen use efficiency (NUE), phosphate use efficiency (PUE) andpotassium use efficiency (KUE) are assessed, checking the ability of thetransgenic plants to thrive under nutrient restraining conditions.

Nitrogen use efficiency—To analyze whether the transgenic plants (e.g.,Arabidopsis plants) are more responsive to nitrogen, plant are grown in0.75-3 mM (nitrogen deficient conditions) or 6-10 mM (optimal nitrogenconcentration). Plants are allowed to grow for additional 25 days oruntil seed production. The plants are then analyzed for their overallsize, time to flowering, yield, protein content of shoot and/orgrain/seed production. The parameters checked can be the overall size ofthe plant, wet and dry weight, the weight of the seeds yielded, theaverage seed size and the number of seeds produced per plant. Otherparameters that may be tested are: the chlorophyll content of leaves (asnitrogen plant status and the degree of leaf greenness is highlycorrelated), amino acid and the total protein content of the seeds orother plant parts such as leaves or shoots and oil content. Transformedplants not exhibiting substantial physiological and/or morphologicaleffects, or exhibiting higher measured parameters levels than wild-typeplants, are identified as nitrogen use efficient plants.

Nitrogen Use efficiency assay using plantlets—The assay is doneaccording to Yanagisawa-S. et al. with minor modifications (“Metabolicengineering with Dof1 transcription factor in plants: Improved nitrogenassimilation and growth under low-nitrogen conditions” Proc. Natl. Acad.Sci. USA 101, 7833-7838). Briefly, transgenic plants which are grown for7-10 days in 0.5×MS [Murashige-Skoog] supplemented with a selectionagent are transferred to two nitrogen-limiting conditions: MS media inwhich the combined nitrogen concentration (NH₄NO₃ and KNO₃) was 0.75 mM(nitrogen deficient conditions) or 6-15 mM (optimal nitrogenconcentration). Plants are allowed to grow for additional 30-40 days andthen photographed, individually removed from the Agar (the shoot withoutthe roots) and immediately weighed (fresh weight) for later statisticalanalysis. Constructs for which only T1 seeds are available are sown onselective media and at least 20 seedlings (each one representing anindependent transformation event) are carefully transferred to thenitrogen-limiting media. For constructs for which T2 seeds areavailable, different transformation events are analyzed. Usually, 20randomly selected plants from each event are transferred to thenitrogen-limiting media allowed to grow for 3-4 additional weeks andindividually weighed at the end of that period. Transgenic plants arecompared to control plants grown in parallel under the same conditions.Mock-transgenic plants expressing the uidA reporter gene (GUS) under thesame promoter or transgenic plants carrying the same promoter butlacking a reporter gene are used as control.

Nitrogen determination—The procedure for N (nitrogen) concentrationdetermination in the structural parts of the plants involves thepotassium persulfate digestion method to convert organic N to NO₃(Purcell and King 1996 Argon. J, 88:111-113, the modified Cd⁻ mediatedreduction of NO₃ ⁻ to NO₂ ⁻ (Vodovotz 1996 Biotechniques 20:390-394) andthe measurement of nitrite by the Griess assay (Vodovotz 1996, supra).The absorbance values are measured at 550 nm against a standard curve ofNaNO₂. The procedure is described in details in Samonte et al. 2006Agron. J. 98:168-176.

Germination tests—Germination tests compare the percentage of seeds fromtransgenic plants that could complete the germination process to thepercentage of seeds from control plants that are treated in the samemanner. Normal conditions are considered for example, incubations at 22°C. under 22-hour light 2-hour dark daily cycles. Evaluation ofgermination and seedling vigor is conducted between 4 and 14 days afterplanting. The basal media is 50% MS medium (Murashige and Skoog, 1962Plant Physiology 15, 473-497).

Germination is checked also at unfavorable conditions such as cold(incubating at temperatures lower than 10° C. instead of 22° C.) orusing seed inhibition solutions that contain high concentrations of anosmolyte such as sorbitol (at concentrations of 50 mM, 100 mM, 200 mM,300 mM, 500 mM, and up to 1000 mM) or applying increasing concentrationsof salt (of 50 mM, 100 mM, 200 mM, 300 mM, 500 mM NaCl).

The effect of the transgene on plant's vigor, growth rate, biomass,yield and/or oil content can be determined using known methods.

Plant vigor—The plant vigor can be calculated by the increase in growthparameters such as leaf area, fiber length, rosette diameter, plantfresh weight and the like per time.

Growth rate—The growth rate can be measured using digital analysis ofgrowing plants. For example, images of plants growing in greenhouse onplot basis can be captured every 3 days and the rosette area can becalculated by digital analysis. Rosette area growth is calculated usingthe difference of rosette area between days of sampling divided by thedifference in days between samples.

Evaluation of growth rate can be done by measuring plant biomassproduced, rosette area, leaf size or root length per time (can bemeasured in cm² per day of leaf area).

Relative growth area can be calculated using Formula II.

Relative growth rate area=Regression coefficient of area along timecourse.  Formula II:

Thus, the relative growth area rate is in units of area units (e.g.,mm²/day or cm²/day) and the relative length growth rate is in units oflength units (e.g., cm/day or mm/day).

For example, RGR can be determined for plant height (Formula III), SPAD(Formula IV), Number of tillers (Formula V), root length (Formula VI),vegetative growth (Formula VII), leaf number (Formula VIII), rosettearea (Formula IX), rosette diameter (Formula X), plot coverage (FormulaXI), leaf blade area (Formula XII), and leaf area (Formula XIII).

Relative growth rate of Plant height=Regression coefficient of Plantheight along time course (measured in cm/day).  Formula III:

Relative growth rate of SPAD=Regression coefficient of SPAD measurementsalong time course.  Formula IV:

Relative growth rate of Number of tillers=Regression coefficient ofNumber of tillers along time course (measured in units of “number oftillers/day”).  Formula V:

Relative growth rate of root length=Regression coefficient of rootlength along time course (measured in cm per day).  Formula VI:

Vegetative growth rate analysis—was calculated according to Formula VIIbelow.

Relative growth rate of vegetative growth=Regression coefficient ofvegetative dry weight along time course (measured in grams perday).  Formula VII:

Relative growth rate of leaf number=Regression coefficient of leafnumber along time course (measured in number per day).  Formula VIII:

Relative growth rate of rosette area=Regression coefficient of rosettearea along time course (measured in cm² per day).  Formula IX:

Relative growth rate of rosette diameter=Regression coefficient ofrosette diameter along time course (measured in cm per day).  Formula X:

Relative growth rate of plot coverage=Regression coefficient of plot(measured in cm² per day).  Formula XI:

Relative growth rate of leaf blade area=Regression coefficient of leafarea along time course (measured in cm² per day).  Formula XII:

Relative growth rate of leaf area=Regression coefficient of leaf areaalong time course (measured in cm² per day).  Formula XIII:

1000 Seed Weight=number of seed in sample/sample weight×1000  FormulaXIV:

The Harvest Index can be calculated using Formulas XV, XVI, XVII, XVIIIand LXV below.

Harvest Index (seed)=Average seed yield per plant/Average dryweight.  Formula XV:

Harvest Index (Sorghum)=Average grain dry weight per Head/(Averagevegetative dry weight per Head+Average Head dry weight)  Formula XVI:

Harvest Index (Maize)=Average grain weight per plant/(Average vegetativedry weight per plant plus Average grain weight per plant)  Formula XVII:

Harvest Index (for barley)—The harvest index is calculated using FormulaXVIII.

Harvest Index (for barley and wheat)=Average spike dry weight perplant/(Average vegetative dry weight per plant+Average spike dry weightper plant)  Formula XVIII:

Following is a non-limited list of additional parameters which can bedetected in order to show the effect of the transgene on the desiredplant's traits:

Grain circularity=4×3.14 (grain area/perimeter)  Formula XIX:

Internode volume=3.14×(d/2)²×1  Formula XX:

Total dry matter (kg)=Normalized head weight per plant+vegetative dryweight.  Formula XXI:

Root/Shoot Ratio=total weight of the root at harvest/total weight of thevegetative portion above ground at harvest. (=RBiH/BiH)  Formula XXII:

Ratio of the number of pods per node on main stem at pod set=Totalnumber of pods on main stem/Total number of nodes on main stem.  FormulaXXIII:

Ratio of total number of seeds in main stem to number of seeds onlateral branches=Total number of seeds on main stem at pod set/Totalnumber of seeds on lateral branches at pod set.  Formula XXIV:

Petiole Relative Area=(Petiole area)/Rosette area (measured in %b).  Formula XXV:

% reproductive tiller percentage=Number of Reproductive tillers/numberof tillers)×100.  Formula XXVI:

Spikes Index=Average Spikes weight per plant/(Average vegetative dryweight per plant plus Average Spikes weight per plant).  Formula XXVII:

Relative growth rate of root coverage=Regression coefficient of rootcoverage along time course.  Formula XXVIII:

Seed Oil yield=Seed yield per plant (gr.)*Oil % in seed.  Formula XXIX:

shoot/root Ratio=total weight of the vegetative portion above ground atharvest/total weight of the root at harvest.  Formula XXX:

Spikelets Index=Average Spikelets weight per plant/(Average vegetativedry weight per plant plus Average Spikelets weight per plant).  FormulaXXXI:

% Canopy coverage=(1−(PAR_DOWN/PAR_UP))×100 measured using AccuPARCeptometer Model LP-80.  Formula XXXII:

leaf mass fraction=Leaf area/shoot FW.  Formula XXXIII:

Relative growth rate based on dry weight=Regression coefficient of dryweight along time course.  Formula XXXIV:

Dry matter partitioning (ratio)—At the end of the growing period 6plants heads as well as the rest of the plot heads were collected,threshed and grains were weighted to obtain grains yield per plot. Drymatter partitioning was calculated by dividing grains yield per plot tovegetative dry weight per plot.  Formula XXXV:

1000 grain weight filling rate (gr/day)—The rate of grain filling wascalculated by dividing 1000 grain weight by grain fillduration.  Formula XXXVI:

Specific leaf area (cm²/gr)—Leaves were scanned to obtain leaf area perplant, and then were dried in an oven to obtain the leaves dry weight.Specific leaf area was calculated by dividing the leaf area by leaf dryweight.  Formula XXXVII:

Vegetative dry weight per plant at flowering/water until flowering(gr/lit)—Calculated by dividing vegetative dry weight (excluding rootsand reproductive organs) per plant at flowering by the water used forirrigation up to flowering.  Formula XXXVIII:

Yield filling rate (gr/day)—The rate of grain filling was calculated bydividing grains Yield by grain fill duration.  Formula XXXIX:

Yield per dunam/water until tan (kg/lit)—Calculated by dividing Grainsyield per dunam by water used for irrigation until tan.  Formula XXXX:

Yield per plant/water until tan (gr/lit)—Calculated by dividing Grainsyield per plant by water used for irrigation until tan.  Formula XXXXI:

Yield per dunam/water until maturity (gr/lit)—Calculated by dividinggrains yield per dunam by the water used for irrigation up tomaturity.  Formula XXXXII:

Vegetative dry weight per plant/water until maturity (gr/lit):Calculated by dividing vegetative dry weight per plant (excluding rootsand reproductive organs) at harvest by the water used for irrigation upto maturity.  Formula XXXXIII:

Total dry matter per plant/water until maturity (gr/lit): Calculated bydividing total dry matter at harvest (vegetative and reproductive,excluding roots) per plant by the water used for irrigation up tomaturity.  Formula XXXXIV:

Total dry matter per plant/water until maturity (gr/lit): Calculated bydividing total dry matter at flowering (vegetative and reproductive,excluding roots) per plant by the water used for irrigation up toflowering.  Formula XXXXV:

Heads index (ratio): Average heads weight/(Average vegetative dry weightper plant plus Average heads weight per plant).  Formula XXXXVI:

Yield/SPAD (kg/SPAD units)—Calculated by dividing grains yield byaverage SPAD measurements per plot.  Formula XXXXVII:

Stem water content (percentage)—stems were collected and fresh weight(FW) was weighted. Then the stems were oven dry and dry weight (DW) wasrecorded. Stems dry weight was divided by stems fresh weight, subtractedfrom 1 and multiplied by 100.  Formula XXXXVIII:

Leaf water content (percentage)—Leaves were collected and fresh weight(FW) was weighted. Then the leaves were oven dry and dry weight (DW) wasrecorded. Leaves dry weight was divided by leaves fresh weight,subtracted from 1 and multiplied by 100.  Formula XXXXIX:

stem volume (cm{circumflex over ( )}3)—The average stem volume wascalculated by multiplying the average stem length by (3.14*((mean lowerand upper stem width)/2){circumflex over ( )}2).  Formula L:

NUE—is the ratio between total grain yield per total nitrogen(applied+content) in soil.  Formula LI:

NUpE—Is the ratio between total plant N content per total N(applied+content) in soil.  Formula LII:

Total NUtE—Is the ratio between total dry matter per N content of totaldry matter.  Formula LIII:

Stem density—is the ratio between internode dry weight and internodevolume.  Formula LIV:

Grain NUE—Is the ratio between grain yield per N content of total drymatter  Formula LV:

N harvest index (Ratio)—Is the ratio between nitrogen content in grainper plant and the nitrogen of whole plant at harvest.  Formula LVI:

Biomass production efficiency—is the ratio between plant biomass andtotal shoot N.  Formula LVII:

Harvest index (plot)(ratio)—Average seed yield per plot/Average dryweight per plot.  Formula LVIII:

Relative growth rate of petiole relative area—Regression coefficient ofpetiole relative area along time course (measured in cm2 perday).  Formula LIX:

Yield per spike filling rate (gr/day)—spike filling rate was calculatedby dividing grains yield per spike to grain fill duration.  Formula LX:

Yield per micro plots filling rate (gr/day)—micro plots filling rate wascalculated by dividing grains yield per micro plots to grain fillduration.  Formula LXI:

Grains yield per hectare [ton/ha]—all spikes per plot were harvestedthreshed and grains were weighted after sun dry. The resulting value wasdivided by the number of square meters and multiplied by 10,000 (10,000square meters=1 hectare).  Formula LXII:

Total dry matter (for Maize)=Normalized ear weight per plant+vegetativedry weight.  Formula LXIII:

$\begin{matrix}{{{Agronomical}\mspace{14mu} {NUE}} = \frac{\begin{matrix}{{{Yield}\mspace{14mu} {per}\mspace{14mu} {plant}\mspace{14mu} ( {{Kg}.} )^{X\mspace{14mu} {Nitrogen}\mspace{14mu} {Fertilization}}} -} \\{{Yield}\mspace{14mu} {per}\mspace{14mu} {plant}\mspace{14mu} ( {{Kg}.} )^{0\% \mspace{14mu} {Nitrogen}\mspace{14mu} {Fertilization}}}\end{matrix}}{{Fertilizer}^{X}}} & {{Formula}\mspace{14mu} {LXIV}}\end{matrix}$Harvest Index (brachypodium)=Average grain weight/average dry(vegetative+spikelet) weight per plant.  Formula LXV:

Harvest Index for Sorghum* (* when the plants were not dried)=FW (freshweight) Heads/(FW Heads+FW Plants)  Formula LXVI:

Grain protein concentration—Grain protein content (g grain protein m⁻²)is estimated as the product of the mass of grain N (g grain N m²)multiplied by the N/protein conversion ratio of k−5.13 (Mosse 1990,supra). The grain protein concentration is estimated as the ratio ofgrain protein content per unit mass of the grain (g grain protein kg⁻¹grain).

Fiber length—Fiber length can be measured using fibrograph. Thefibrograph system was used to compute length in terms of “Upper HalfMean” length. The upper half mean (UHM) is the average length of longerhalf of the fiber distribution. The fibrograph measures length in spanlengths at a given percentage point (cottoninc (dot)com/ClassificationofCotton/?Pg=4#Length).

According to some embodiments of the invention, increased yield of cornmay be manifested as one or more of the following: increase in thenumber of plants per growing area, increase in the number of ears perplant, increase in the number of rows per ear, number of kernels per earrow, kernel weight, thousand kernel weight (1000-weight), earlength/diameter, increase oil content per kernel and increase starchcontent per kernel.

As mentioned, the increase of plant yield can be determined by variousparameters. For example, increased yield of rice may be manifested by anincrease in one or more of the following: number of plants per growingarea, number of panicles per plant, number of spikelets per panicle,number of flowers per panicle, increase in the seed filling rate,increase in thousand kernel weight (1000-weight), increase oil contentper seed, increase starch content per seed, among others. An increase inyield may also result in modified architecture, or may occur because ofmodified architecture.

Similarly, increased yield of soybean may be manifested by an increasein one or more of the following: number of plants per growing area,number of pods per plant, number of seeds per pod, increase in the seedfilling rate, increase in thousand seed weight (1000-weight), reduce podshattering, increase oil content per seed, increase protein content perseed, among others. An increase in yield may also result in modifiedarchitecture, or may occur because of modified architecture.

Increased yield of canola may be manifested by an increase in one ormore of the following: number of plants per growing area, number of podsper plant, number of seeds per pod, increase in the seed filling rate,increase in thousand seed weight (1000-weight), reduce pod shattering,increase oil content per seed, among others. An increase in yield mayalso result in modified architecture, or may occur because of modifiedarchitecture.

Increased yield of cotton may be manifested by an increase in one ormore of the following: number of plants per growing area, number ofbolls per plant, number of seeds per boll, increase in the seed fillingrate, increase in thousand seed weight (1000-weight), increase oilcontent per seed, improve fiber length, fiber strength, among others. Anincrease in yield may also result in modified architecture, or may occurbecause of modified architecture.

Oil content—The oil content of a plant can be determined by extractionof the oil from the seed or the vegetative portion of the plant.Briefly, lipids (oil) can be removed from the plant (e.g., seed) bygrinding the plant tissue in the presence of specific solvents (e.g.,hexane or petroleum ether) and extracting the oil in a continuousextractor. Indirect oil content analysis can be carried out usingvarious known methods such as Nuclear Magnetic Resonance (NMR)Spectroscopy, which measures the resonance energy absorbed by hydrogenatoms in the liquid state of the sample [See for example, Conway T F,and Earle F R., 1963, Journal of the American Oil Chemists' Society;Springer Berlin/Heidelberg. ISSN: 0003-021X (Print) 1558-9331 (Online)];the Near Infrared (NI) Spectroscopy, which utilizes the absorption ofnear infrared energy (0.1100-2500 nm) by the sample; and a methoddescribed in WO/2001/023884, which is based on extracting oil a solvent,evaporating the solvent in a gas stream which forms oil particles, anddirecting a light into the gas stream and oil particles which forms adetectable reflected light.

Thus, the present invention is of high agricultural value for promotingthe yield of commercially desired crops (e.g., biomass of vegetativeorgan such as poplar wood, or reproductive organ such as number of seedsor seed biomass).

Any of the transgenic plants described hereinabove or parts thereof maybe processed to produce a feed, meal, protein or oil preparation, suchas for ruminant animals.

The transgenic plants described hereinabove, which exhibit an increasedoil content can be used to produce plant oil (by extracting the oil fromthe plant).

The plant oil (including the seed oil and/or the vegetative portion oil)produced according to the method of the invention may be combined with avariety of other ingredients. The specific ingredients included in aproduct are determined according to the intended use. Exemplary productsinclude animal feed, raw material for chemical modification,biodegradable plastic, blended food product, edible oil, biofuel,cooking oil, lubricant, biodiesel, snack food, cosmetics, andfermentation process raw material, Exemplary products to be incorporatedto the plant oil include animal feeds, human food products such asextruded snack foods, breads, as a food binding agent, aquaculturefeeds, fermentable mixtures, food supplements, sport drinks, nutritionalfood bars, multi-vitamin supplements, diet drinks, and cereal foods.

According to some embodiments of the invention, the oil comprises a seedoil.

According to some embodiments of the invention, the oil comprises avegetative portion oil (oil of the vegetative portion of the plant).

According to some embodiments of the invention, the plant cell forms apart of a plant.

According to another embodiment of the present invention, there isprovided a food or feed comprising the plants or a portion thereof ofthe present invention.

As used herein the term “about” refers to ±10%.

The terms “comprises”, “comprising”, “includes”, “including”, “having”and their conjugates mean “including but not limited to”.

The term “consisting of” means “including and limited to”.

The term “consisting essentially of” means that the composition, methodor structure may include additional ingredients, steps and/or parts, butonly if the additional ingredients, steps and/or parts do not materiallyalter the basic and novel characteristics of the claimed composition,method or structure.

As used herein, the singular form “a”, “an” and “the” include pluralreferences unless the context clearly dictates otherwise. For example,the term “a compound” or “at least one compound” may include a pluralityof compounds, including mixtures thereof.

Throughout this application, various embodiments of this invention maybe presented in a range format. It should be understood that thedescription in range format is merely for convenience and brevity andshould not be construed as an inflexible limitation on the scope of theinvention. Accordingly, the description of a range should be consideredto have specifically disclosed all the possible subranges as well asindividual numerical values within that range. For example, descriptionof a range such as from 1 to 6 should be considered to have specificallydisclosed subranges such as from 1 to 3, from 1 to 4, from 1 to 5, from2 to 4, from 2 to 6, from 3 to 6 etc., as well as individual numberswithin that range, for example, 1, 2, 3, 4, 5, and 6. This appliesregardless of the breadth of the range.

Whenever a numerical range is indicated herein, it is meant to includeany cited numeral (fractional or integral) within the indicated range.The phrases “ranging/ranges between” a first indicate number and asecond indicate number and “ranging/ranges from” a first indicate number“to” a second indicate number are used herein interchangeably and aremeant to include the first and second indicated numbers and all thefractional and integral numerals therebetween.

As used herein the term “method” refers to manners, means, techniquesand procedures for accomplishing a given task including, but not limitedto, those manners, means, techniques and procedures either known to, orreadily developed from known manners, means, techniques and proceduresby practitioners of the chemical, pharmacological, biological,biochemical and medical arts.

When reference is made to particular sequence listings, such referenceis to be understood to also encompass sequences that substantiallycorrespond to its complementary sequence as including minor sequencevariations, resulting from, e.g., sequencing errors, cloning errors, orother alterations resulting in base substitution, base deletion or baseaddition, provided that the frequency of such variations is less than 1in 50 nucleotides, alternatively, less than 1 in 100 nucleotides,alternatively, less than 1 in 200 nucleotides, alternatively, less than1 in 500 nucleotides, alternatively, less than 1 in 1000 nucleotides,alternatively, less than 1 in 5,000 nucleotides, alternatively, lessthan 1 in 10.000 nucleotides.

It is appreciated that certain features of the invention, which are, forclarity, described in the context of separate embodiments, may also beprovided in combination in a single embodiment. Conversely, variousfeatures of the invention, which are, for brevity, described in thecontext of a single embodiment, may also be provided separately or inany suitable subcombination or as suitable in any other describedembodiment of the invention. Certain features described in the contextof various embodiments are not to be considered essential features ofthose embodiments, unless the embodiment is inoperative without thoseelements.

Various embodiments and aspects of the present invention as delineatedhereinabove and as claimed in the claims section below find experimentalsupport in the following examples.

EXAMPLES

Reference is now made to the following examples, which together with theabove descriptions illustrate some embodiments of the invention in a nonlimiting fashion.

Generally, the nomenclature used herein and the laboratory proceduresutilized in the present invention include molecular, biochemical,microbiological and recombinant DNA techniques. Such techniques arethoroughly explained in the literature. See, for example, “MolecularCloning: A laboratory Manual” Sambrook et al., (1989); “CurrentProtocols in Molecular Biology” Volumes I-III Ausubel, R. M., ed.(1994); Ausubel et al., “Current Protocols in Molecular Biology”, JohnWiley and Sons. Baltimore, Md. (1989); Perbal, “A Practical Guide toMolecular Cloning”, John Wiley & Sons, New York (1988); Watson et al.,“Recombinant DNA”, Scientific American Books, New York: Birren et al.(eds) “Genome Analysis: A Laboratory Manual Series”. Vols. 1-4, ColdSpring Harbor Laboratory Press, New York (1998); methodologies as setforth in U.S. Pat. Nos. 4,666,828; 4,683,202; 4,801,531; 5,192,659 and5,272,057; “Cell Biology: A Laboratory Handbook”. Volumes I-III Cellis,J. E., ed. (1994); “Current Protocols in Immunology” Volumes I-IIIColigan J. E., ed. (1994); Stites et al. (eds). “Basic and ClinicalImmunology” (8th Edition), Appleton & Lange, Norwalk, Conn. (1994);Mishell and Shiigi (eds), “Selected Methods in Cellular Immunology”. W.H. Freeman and Co., New York (1980); available immunoassays areextensively described in the patent and scientific literature, see, forexample, U.S. Pat. Nos. 3,791,932; 3,839,153; 3,850,752; 3,850,578;3,853,987; 3,867,517; 3,879,262; 3,901,654; 3,935,074; 3,984,533;3,996,345; 4,034,074; 4,098,876; 4,879,219; 5,011,771 and 5,281,521;“Oligonucleotide Synthesis” Gait, M. J., ed. (1984); “Nucleic AcidHybridization” Hames, B. D., and Higgins S. J., eds. (1985);“Transcription and Translation” Hames, B. D., and Higgins S. J., Eds.(0.1984); “Animal Cell Culture” Freshney, R. I., ed. (1986);“Immobilized Cells and Enzymes” IRL Press. (1986); “A Practical Guide toMolecular Cloning” Perbal, B., (1984) and “Methods in Enzymology” Vol.1-317. Academic Press; “PCR Protocols: A Guide To Methods AndApplications”, Academic Press. San Diego, Calif. (1990); Marshak et al.,“Strategies for Protein Purification and Characterization—A LaboratoryCourse Manual” CSHL Press (1996); all of which are incorporated byreference as if fully set forth herein. Other general references areprovided throughout this document. The procedures therein are believedto be well known in the art and are provided for the convenience of thereader. All the information contained therein is incorporated herein byreference.

General Experimental and Bioinformatics Methods

RNA extraction—Tissues growing at various growth conditions (asdescribed below) were sampled and RNA was extracted using TRIzol Reagentfrom Invitrogen [invitrogen (dot) com/content (dot)cfm?pageid=469].Approximately 30-50 mg of tissue was taken from samples. The weighedtissues were ground using pestle and mortar in liquid nitrogen andresuspended in 500 W of TRIzol Reagent. To the homogenized lysate, 100μl of chloroform was added followed by precipitation using isopropanoland two washes with 75% ethanol. The RNA was eluted in 30 μl ofRNase-free water. RNA samples were cleaned up using Qiagen's RNeasyminikit clean-up protocol as per the manufacturer's protocol (QIAGENInc, CA USA). For convenience, each micro-array expression informationtissue type has received an expression Set ID.

Correlation analysis—was performed for selected genes according to someembodiments of the invention, in which the characterized parameters(measured parameters according to the correlation IDs) were used as “xaxis” for correlation with the tissue transcriptome, which was used asthe “Y axis”. For each gene and measured parameter a correlationcoefficient “R” was calculated (using Pearson correlation) along with ap-value for the significance of the correlation. When the correlationcoefficient (R) between the levels of a gene's expression in a certaintissue and a phenotypic performance across ecotypes/variety/hybrid ishigh in absolute value (between 0.5-1), there is an association betweenthe gene (specifically the expression level of this gene) the phenotypiccharacteristic (e.g., improved yield, growth rate, nitrogen useefficiency, abiotic stress tolerance and the like).

Example 1 Identifying Genes which Improve Yield and AgronomicalImportant Traits in Plants

The present inventors have identified polynucleotides which expressionthereof in plants can increase yield, fiber yield, fiber quality,photosynthetic capacity, growth rate, vigor, biomass, oil content,abiotic stress tolerance (ABST), fertilizer use efficiency (FUE) such asnitrogen use efficiency (NUE), and water use efficiency (WUE) of aplant, as follows.

All nucleotide sequence datasets used here were originated from publiclyavailable databases or from performing sequencing using the Solexatechnology (e.g. Barley and Sorghum). Sequence data from 100 differentplant species was introduced into a single, comprehensive database.Other information on gene expression, protein annotation, enzymes andpathways were also incorporated.

Major databases used include:

Genomes

-   -   Arabidopsis genome [TAIR genome version 6 (arabidopsis (dot)        org/)]    -   Rice genome [IRGSP build 4.0 (rgp (dot) dna (dot) affrc (dot) go        (dot) jp/IRGSP/)].    -   Poplar [Populus trichocarpa release 1.1 from JGI (assembly        release v1.0) (genome (dot) jgi-psf (dot) org/)]    -   Brachypodium [JGI 4× assembly, brachpodium (dot) org)]    -   Soybean [DOE-JGI SCP, version Glyma0 (phytozome (dot) net/)]    -   Grape [French-Italian Public Consortium for Grapevine Genome        Characterization grapevine genome (genoscope (dot) cns (dot) fr        /)]    -   Castobean [TIGR/J Craig Venter Institute 4× assembly [msc (dot)        jcvi (dot) org/r communis]    -   Sorghum [DOE-JGI SCP, version Sbi1 [phytozome (dot) net/)].    -   Partially assembled genome of Maize [maizesequence (dot) org/]

Expressed EST and mRNA Sequences were Extracted from the FollowingDatabases:

-   -   GenBank (ncbi (dot) nlm (dot) nih (dot) gov/dbEST)    -   RefSeq (ncbi (dot) nlm (dot) nih (dot) gov/RefSeq/).    -   TAIR (arabidopsis (dot) org/).

Protein and Pathway Databases

-   -   Uniprot [uniprot (dot) org/].    -   AraCyc [arabidopsis (dot) org/biocyc/index (dot) jsp].    -   ENZYME [expasy (dot) org/enzyme/].    -   Microarray datasets were downloaded from:    -   GEO (ncbi(dot) nlm. (dot) nih (dot) gov/geo/)    -   TAIR (Arabidopsis (dot) org/).    -   Proprietary microarray data (WO2008/122980).

QTL and SNPs Information

-   -   Gramene [gramene (dot) org/qtl/].    -   Panzea [panzea (dot) org/index (dot) html].

Database Assembly—was performed to build a wide, rich, reliableannotated and easy to analyze database comprised of publicly availablegenomic mRNA. ESTs DNA sequences, data from various crops as well asgene expression, protein annotation and pathway data QTLs. and otherrelevant information.

Database assembly is comprised of a toolbox of gene refining,structuring, annotation and analysis tools enabling to construct atailored database for each gene discovery project. Gene refining andstructuring tools enable to reliably detect splice variants andantisense transcripts, generating understanding of various potentialphenotypic outcomes of a single gene. The capabilities of the “LEADS”platform of Compugen LTD for analyzing human genome have been confirmedand accepted by the scientific community [see e.g., “WidespreadAntisense Transcription”, Yelin, et al. (2003) Nature Biotechnology 21,379-85; “Splicing of Alu Sequences”, Lev-Maor, et al. (2003) Science 300(5623), 1288-91; “Computational analysis of alternative splicing usingEST tissue information”, Xie H et al. Genomics 2002], and have beenproven most efficient in plant genomics as well.

EST clustering and gene assembly—For gene clustering and assembly oforganisms with available genome sequence data (arabidopsis, rice,castorbean, grape, brachypodium, poplar, soybean, sorghum) the genomicLEADS version (GANG) was employed. This tool allows most accurateclustering of ESTs and mRNA sequences on genome, and predicts genestructure as well as alternative splicing events and anti-sensetranscription.

For organisms with no available full genome sequence data, “expressedLEADS” clustering software was applied.

Gene annotation—Predicted genes and proteins were annotated as follows:

Blast search [blast (dot) ncbi (dot) nlm (dot) nih (dot) gov/Blast (dot)cgi] against all plant UniProt [uniprot (dot) org/] sequences wasperformed. Open reading frames of each putative transcript were analyzedand longest ORF with higher number of homologues was selected aspredicted protein of the transcript. The predicted proteins wereanalyzed by InterPro [ebi (dot) ac (dot) uk/interpro/].

Blast against proteins from AraCyc and ENZYME databases was used to mapthe predicted transcripts to AraCyc pathways.

Predicted proteins from different species were compared using blastalgorithm [ncbi (dot) nlm (dot) nih (dot) gov/Blast (dot) cgi] tovalidate the accuracy of the predicted protein sequence, and forefficient detection of orthologs.

Gene expression profiling—Several data sources were exploited for geneexpression profiling, namely microarray data and digital expressionprofile (see below). According to gene expression profile, a correlationanalysis was performed to identify genes which are co-regulated underdifferent development stages and environmental conditions and associatedwith different phenotypes.

Publicly available microarray datasets were downloaded from TAIR andNCBI GEO sites, renormalized, and integrated into the database.Expression profiling is one of the most important resource data foridentifying genes important for yield.

A digital expression profile summary was compiled for each clusteraccording to all keywords included in the sequence records comprisingthe cluster. Digital expression, also known as electronic Northern Blot,is a tool that displays virtual expression profile based on the ESTsequences forming the gene cluster. The tool provides the expressionprofile of a cluster in terms of plant anatomy (e.g., the tissue/organin which the gene is expressed), developmental stage (the developmentalstages at which a gene can be found) and profile of treatment (providesthe physiological conditions under which a gene is expressed such asdrought, cold, pathogen infection, etc). Given a random distribution ofESTs in the different clusters, the digital expression provides aprobability value that describes the probability of a cluster having atotal of N ESTs to contain X ESTs from a certain collection oflibraries. For the probability calculations, the following is taken intoconsideration: a) the number of ESTs in the cluster, b) the number ofESTs of the implicated and related libraries, c) the overall number ofESTs available representing the species. Thereby clusters with lowprobability values are highly enriched with ESTs from the group oflibraries of interest indicating a specialized expression.

Recently, the accuracy of this system was demonstrated by Portnoy etal., 2009 (Analysis Of The Melon Fruit Transcriptome Based On 454Pyrosequencing) in: Plant & Animal Genomes XVI IConference, San Diego,Calif. Transcriptomeic analysis, based on relative EST abundance in datawas performed by 454 pyrosequencing of cDNA representing mRNA of themelon fruit. Fourteen double strand cDNA samples obtained from twogenotypes, two fruit tissues (flesh and rind) and four developmentalstages were sequenced. GS FLX pyrosequencing (Roche/454 Life Sciences)of non-normalized and purified cDNA samples yielded 1,150,657 expressedsequence tags, that assembled into 67,477 unigenes (32,357 singletonsand 35,120 contigs). Analysis of the data obtained against the CucurbitGenomics Database licugi (dot) org/J confirmed the accuracy of thesequencing and assembly. Expression patterns of selected genes fittedwell their qRT-PCR data.

Overall, 220 genes (SEQ ID NOs: 1-473 for polynucleotides and SEQ IDNOs: 474-760 for polypeptides) were identified to have a major impact onplant yield, growth rate, photosynthetic capacity, vigor, biomass,growth rate, oil content, abiotic stress tolerance, nitrogen useefficiency, water use efficiency and fertilizer use efficiency whenexpression thereof is increased in plants (e.g., in plantsover-expressing the polynucleotides/polypeptides). The identified genes,their curated polynucleotide and polypeptide sequences, as well as theirupdated sequences according to GenBank database are summarized in Table1, hereinbelow.

TABLE 1 Identified genes for increasing yield, growth rate, vigor,biomass, growth rate, oil content, abiotic stress tolerance, nitrogenuse efficiency, water use efficiency and fertilizer use efficiency of aplant Polyn. Polyp. SEQ SEQ ID ID Gene Name Organism / Cluster Name NO:NO: LYM1010 barley|10v2|BE411105 1 474 LYM1011 barley|10v2|BE421247 2475 LYM1012 barley|10v2|BE456034 3 476 LYM1013 barley|10v2|BE601902 4477 LYM1014 barley|10v2|BF621691 5 478 LYM1015 barley|10v2|BF625619 6479 LYM1016 barley|12v1|AJ303113 7 480 LYM1017 barley|12v1|AJ460503 8481 LYM1018 barley|12v1|AJ462240 9 482 LYM1019 barley|12v1|AJ462593 10483 LYM1020 barley|12v1|AJ465036 11 484 LYM1021 barley|12v1|AJ472697 12485 LYM1022 barley|12v1|AJ476944 13 486 LYM1023 barley|12v1|AV832509 14487 LYM1024 barley|12v1|AV832821 15 488 LYM1025 barley|12v1|AV833067 16489 LYM1026 barley|12v1|AV833535 17 490 LYM1027 barley|12v1|AV835246 18491 LYM1028 barley|12v1|AV836250 19 492 LYM1029 barley|12v1|AV919419 20493 LYM1030 barley|12v1|AV932314 21 494 LYM1031 barley|12v1|AV932367 22495 LYM1032 barley|12v1|AV932518 23 496 LYM1033 barley|12v1|BE215699 24497 LYM1034 barley|12v1|BE412462 25 498 LYM1035 barley|12v1|BE413472 26499 LYM1036 barley|12v1|BE420953 27 500 LYM1037 barley|12v1|BE420982 28501 LYM1038 barley|12v1|BE421696 29 502 LYM1040 barley|12v1|BE437999 30503 LYM1041 barley|12v1|BE438048 31 504 LYM1042 barley|12v1|BF255185 32505 LYM1043 barley|12v1|BF259663 33 506 LYM1044 barley|12v1|BF617642 34507 LYM1046 barley|12v1|BF621200 35 508 LYM1047 barley|12v1|BF623265 36509 LYM1048 barley|12v1|BF624214 37 510 LYM1049 barley|12v1|BF625372 38511 LYM1051 barley|12v1|BG309076 39 512 LYM1052 barley|12v1|BG414431 40513 LYM1053 barley|12v1|BI947802 41 514 LYM1054 barley|12v1|BI949533 42515 LYM1055 barley|12v1|BI949678 43 516 LYM1056 barley|12v1|BI950318 44517 LYM1057 barley|12v1|BI951064 45 518 LYM1058 barley|12v1|BI951865 46519 LYM1059 barley|12v1|BI952771 47 520 LYM1060 barley|12v1|BI953477 48521 LYM1061 barley|12v1|BI953521 49 522 LYM1062 barley|12v1|BI954539 50523 LYM1063 barley|12v1|BI955576 51 524 LYM1064 barley|12v1|BI956170 52525 LYM1065 barley|12v1|BI956211 53 526 LYM1066 barley|12v1|BJ448998 54527 LYM1068 barley|12v1|BJ469847 55 528 LYM1069 barley|12v1|BLYCPPHSYS56 529 LYM1070 barley|12v1|BQ465115 57 530 LYM1071 barley|12v1|BU99012858 531 LYM1072 barley|12v1|BU991227 59 532 LYM1073 barley|12v1|CA02326660 533 LYM1074 barley|12v1|CB875234 61 534 LYM1075 barley|12v1|CX62760962 535 LYM1076 barley|12v1|DN160741 63 536 LYM1078brachypodium|12v1|BRADI1G20385 64 537 LYM1079brachypodium|12v1|BRADI1G23900 65 538 LYM1082brachypodium|12v1|BRADI1G69180 66 539 LYM1084brachypodium|12v1|BRADI1G78710 67 540 LYM1085brachypodium|12v1|BRADI2G15900 68 541 LYM1086brachypodium|12v1|BRADI2G21837 69 542 LYM1087brachypodium|12v1|BRADI2G26280 70 543 LYM1088brachypodium|12v1|BRADI2G41510T2 71 544 LYM1089brachypodium|12v1|BRADI2G51480 72 545 LYM1090brachypodium|12v1|BRADI2G56310 73 546 LYM1091brachypodium|12v1|BRADI3G28720 74 547 LYM1092brachypodium|12v1|BRADI3G45820 75 548 LYM1093brachypodium|12v1|BRADI3G55550 76 549 LYM1094brachypodium|12v1|BRADI4G01140 77 550 LYM1095brachypodium|12v1|BRADI4G29320 78 551 LYM1096brachypodium|12v1|BRADI4G29780 79 552 LYM1097brachypodium|12v1|BRADI4G31900T2 80 553 LYM1098brachypodium|12v1|BRADI5G13190 81 554 LYM1099brachypodium|12v1|BRADI5G25760 82 555 LYM1100foxtail_millet|11v3|EC612472 83 556 LYM1101 foxtail_millet|11v3|EC61294684 557 LYM1102 foxtail_millet|11v3|GT091058 85 558 LYM1103foxtail_millet|11v3|PHY7SI000771M 86 559 LYM1104foxtail_millet|11v3|PHY7SI001398M 87 560 LYM1105foxtail_millet|11v3|PHY7SI002608M 88 561 LYM1106foxtail_millet|11v3|PHY7SI003001M 89 562 LYM1107foxtail_millet|11v3|PHY7SI006370M 90 563 LYM1108foxtail_millet|11v3|PHY7SI006690M 91 564 LYM1109foxtail_millet|11v3|PHY7SI010388M 92 565 LYM1110foxtail_millet|11v3|PHY7SI010853M 93 566 LYM1111foxtail_millet|11v3|PHY7SI014287M 94 567 LYM1112foxtail_millet|11v3|PHY7SI016985M 95 568 LYM1113foxtail_millet|11v3|PHY7SI017913M 96 569 LYM1114foxtail_millet|11v3|PHY7SI020310M 97 570 LYM1115foxtail_millet|11v3|PHY7SI020693M 98 571 LYM1116foxtail_millet|11v3|PHY7SI020984M 99 572 LYM1117foxtail_millet|11v3|PHY7SI023427M 100 573 LYM1118foxtail_millet|11v3|PHY7SI026065M 101 574 LYM1119foxtail_millet|11v3|PHY7SI029337M 102 575 LYM1120foxtail_millet|11v3|PHY7SI030030M 103 576 LYM1121foxtail_millet|11v3|PHY7SI033845M 104 577 LYM1122foxtail_millet|11v3|PHY7SI033950M 105 578 LYM1123foxtail_millet|11v3|PHY7SI034443M 106 579 LYM1124foxtail_millet|11v3|PHY7SI034927M 107 580 LYM1125foxtail_millet|11v3|PHY7SI036157M 108 581 LYM1126foxtail_millet|11v3|PHY7SI036407M 109 582 LYM1127foxtail_millet|11v3|PHY7SI037176M 110 583 LYM1128foxtail_millet|11v3|PHY7SI039767M 111 584 LYM1129 maize|10v1|AA054811112 585 LYM1130 maize|10v1|AA979759 113 586 LYM1131 maize|10v1|AI001351114 587 LYM1132 maize|10v1|AI396444 115 588 LYM1133 maize|10v1|AI600333116 589 LYM1134 maize|10v1|AI621954 117 590 LYM1136 maize|10v1|AI666204118 591 LYM1137 maize|10v1|AI770430 119 592 LYM1138 maize|10v1|AI770478120 593 LYM1139 maize|10v1|AI855153 121 594 LYM1140 maize|10v1|AI901707122 595 LYM1141 maize|10v1|AI920541 123 596 LYM1142 maize|10v1|AI941641124 597 LYM1143 maize|10v1|AI947474 125 598 LYM1146 maize|10v1|AW017808126 599 LYM1149 maize|10v1|AW171826 127 600 LYM1151 maize|10v1|AW313252128 601 LYM1152 maize|10v1|AW438140 129 602 LYM1153 maize|10v1|AW498006130 603 LYM1154 maize|10v1|AW499246 131 604 LYM1155 maize|10v1|AW566480132 605 LYM1156 maize|10v1|AW600668 133 606 LYM1157 maize|10v1|BE056858134 607 LYM1158 maize|10v1|BE511433 135 608 LYM1159 maize|10v1|BE640016136 609 LYM1160 maize|10v1|BG320172 137 610 LYM1161 maize|10v1|BG320248138 611 LYM1162 maize|10v1|BG354201 139 612 LYM1163 maize|10v1|BG462317140 613 LYM1164 maize|10v1|BG833142 141 614 LYM1165 maize|10v1|BG836953142 615 LYM1167 maize|10v1|BM073386 143 616 LYM1168 maize|10v1|BM074116144 617 LYM1169 maize|10v1|BM336987 145 618 LYM1170 maize|10v1|BM379348146 619 LYM1171 maize|10v1|BM498926 147 620 LYM1172 maize|10v1|BQ538346148 621 LYM1173 maize|10v1|CA401866 149 622 LYM1174 maize|10v1|CD433365150 623 LYM1175 maize|10v1|CD651832 151 624 LYM1176 maize|10v1|CD943536152 625 LYM1177 maize|10v1|CD964979 153 626 LYM1178 maize|10v1|CF028527154 627 LYM1179 maize|10v1|CF046250 155 628 LYM1180 maize|10v1|CF244711156 629 LYM1181 maize|10v1|T15274 157 630 LYM1182 maize|10v1|T20342 158631 LYM1183 maize|10v1|T20362 159 632 LYM1184 maize|gb170|AI586584 160633 LYM1185 maize|gb170|AI967089 161 634 LYM1186 maize|gb170|AW267345162 635 LYM1187 maize|gb170|CD997839 163 636 LYM1188 rice|11v1|AU065865164 637 LYM1189 rice|11v1|AU093411 165 638 LYM1190 rice|11v1|AU172407166 639 LYM1191 rice|11v1|BI805353 167 640 LYM1192 rice|11v1|BI806487168 641 LYM1193 rice|11v1|BI812921 169 642 LYM1194 rice|11v1|CA762027170 643 LYM1195 sorghum|12v1|AW677825 171 644 LYM1201sorghum|12v1|SB01G005360 172 645 LYM1202 sorghum|12v1|SB01G006060 173646 LYM1203 sorghum|12v1|SB01G023320 174 647 LYM1204sorghum|12v1|SB01G046820 175 648 LYM1205 sorghum|12v1|SB02G005630 176649 LYM1206 sorghum|12v1|SB02G028130 177 650 LYM1207sorghum|12v1|SB03G011260 178 651 LYM1208 sorghum|12v1|SB03G012430 179652 LYM1209 sorghum|12v1|SB03G043920 180 653 LYM1210sorghum|12v1|SB06G030260 181 654 LYM1211 sorghum|12v1|SB09G006520 182655 LYM1212 sorghum|12v1|SB09G019550 183 656 LYM1213sorghum|12v1|SB09G026150 184 657 LYM1214 sorghum|12v1|SB10G006840 185658 LYM1215 sorghum|13v2|JBIV2SB13002974 186 659 LYM1216soybean|11v1|GLYMA03G35460 187 660 LYM1217 soybean|11v1|GLYMA06G11380188 661 LYM1218 soybean|11v1|GLYMA07G03580 189 662 LYM1219soybean|11v1|GLYMA08G19950 190 663 LYM1220 soybean|11v1|GLYMA09G32550191 664 LYM1221 soybean|11v1|GLYMA13G09620 192 665 LYM1222soybean|11v1|GLYMA13G17510 193 666 LYM1223 soybean|11v1|GLYMA13G21640194 667 LYM1224 soybean|11v1|GLYMA13G39770 195 668 LYM1225soybean|11v1|GLYMA18G52430 196 669 LYM1226 soybean|11v1|GLYMA19G28920197 670 LYM1227 soybean|11v1|GLYMA20G36230 198 671 LYM1228tomato|11v1|AA824906 199 672 LYM1229 tomato|11v1|AF153277 200 673LYM1230 tomato|11v1|AW034456 201 674 LYM1231 tomato|11v1|AW617278 202675 LYM1232 tomato|11v1|BG136313 203 676 LYM1233 tomato|11v1|BG734868204 677 LYM1234 barley|12v1|BE420804 205 678 LYM1235brachypodium|12v1|BRADI1G15290 206 679 LYM1236brachypodium|12v1|BRADI5G10880 207 680 LYM1237brachypodium|12v1|BRADI5G10920 208 681 LYM1239 sorghum|12v1|SB01G004200209 682 LYM1240 tomato|11v1|AW154852 210 683 LYM1032_H1wheat|12v3|CA635514 211 684 LYM1058_H4 maize|10v1|AI649819 212 685LYM1059_H7 maize|10v1|AI977980 213 686 LYM1064_H5 rice|13v2|BE228289 214687 LYM1076_H4 rice|11v1|CA755245 215 688 LYM1091_H5 maize|10v1|AW056217216 689 LYM1101_H3 sorghum|12v1|SB09G025690 217 690 LYM1105_H2sorghum|12v1|SB03G036770 218 691 LYM1164_H1 sorghum|12v1|SB02G009120 219692 LYM1196 sorghum|12v1|CF480531 220 — LYM1019 barley|12v1|AJ462593 221483 LYM1024 barley|12v1|AV832821 223 488 LYM1030 barley|12v1|AV932314225 494 LYM1033 barley|12v1|BE215699 226 497 LYM1037barley|12v1|BE420982 227 501 LYM1046 barley|12v1|BF621200 228 508LYM1057 barley|12v1|BI951064 230 518 LYM1060 barley|12v1|BI953477 231521 LYM1066 barley|12v1|BJ448998 232 527 LYM1078brachypodium|12v1|BRADI1G20385 235 537 LYM1082brachypodium|12v1|BRADI1G69180 236 539 LYM1086brachypodium|12v1|BRADI2G21837 238 542 LYM1088brachypodium|12v1|BRADI2G41510T2 239 544 LYM1096brachypodium|12v1|BRADI4G29780 240 552 LYM1106foxtail_millet|11v3|PHY7SI003001M 242 562 LYM1107foxtail_millet|11v3|PHY7SI006370M 243 563 LYM1113foxtail_millet|11v3|PHY7SI017913M 244 569 LYM1126foxtail_millet|11v3|PHY7SI036407M 247 582 LYM1155 maize|10v1|AW566480251 605 LYM1193 rice|11v1|BI812921 255 642 LYM1205sorghum|12v1|SB02G005630 256 649 LYM1226 soybean|11v1|GLYMA19G28920 262670 LYM1228 tomato|11v1|AA824906 263 672 LYM1076_H4 rice|11v1|CA755245266 688 LYM1091_H5 maize|10v1|AW056217 267 689 LYM1022barley|12v1|AJ476944 222 693 LYM1029 barley|12v1|AV919419 224 694LYM1052 barley|12v1|BG414431 229 695 LYM1071 barley|12v1|BU990128 233696 LYM1074 barley|12v1|CB875234 234 697 LYM1085brachypodium|12v1|BRADI2G15900 237 698 LYM1098brachypodium|12v1|BRADI5G13190 241 699 LYM1119foxtail_millet|11v3|PHY7SI029337M 245 700 LYM1125foxtail_millet|11v3|PHY7SI036157M 246 701 LYM1138 maize|10v1|AI770478248 702 LYM1140 maize|10v1|AI901707 249 703 LYM1142 maize|10v1|AI941641250 704 LYM1163 maize|10v1|BG462317 252 705 LYM1177 maize|10v1|CD964979253 706 LYM1186 maize|gb170|AW267345 254 707 LYM1208sorghum|12v1|SB03G012430 257 708 LYM1214 sorghum|12v1|SB10G006840 258709 LYM1215 sorghum|12v1|SB12V1CRP024405 259 710 LYM1221soybean|11v1|GLYMA13G09620 260 711 LYM1222 soybean|11v1|GLYMA13G17510261 712 LYM1234 barley|12v1|BE420804 264 713 LYM1064_H5rice|11v1|BE228289 265 714 LYM1195 sorghum|12v1|AW677825 268 — LYM1010barley|10v2|BE411105 269 474 LYM1011 barley|10v2|BE421247 270 475LYM1012 barley|10v2|BE456034 271 476 LYM1014 barley|10v2|BF621691 273478 LYM1015 barley|10v2|BF625619 274 479 LYM1016 barley|12v1|AJ303113275 480 LYM1017 barley|12v1|AJ460503 276 481 LYM1019barley|12v1|AJ462593 278 483 LYM1021 barley|12v1|AJ472697 280 485LYM1024 barley|12v1|AV832821 283 488 LYM1025 barley|12v1|AV833067 284489 LYM1026 barley|12v1|AV833535 285 490 LYM1027 barley|12v1|AV835246286 491 LYM1028 barley|12v1|AV836250 287 492 LYM1029barley|12v1|AV919419 288 493 LYM1033 barley|12v1|BE215699 290 497LYM1035 barley|12v1|BE413472 292 499 LYM1036 barley|12v1|BE420953 293500 LYM1037 barley|12v1|BE420982 294 501 LYM1038 barley|12v1|BE421696295 502 LYM1040 barley|12v1|BE437999 296 503 LYM1041barley|12v1|BE438048 297 504 LYM1042 barley|12v1|BF255185 298 505LYM1043 barley|12v1|BF259663 299 506 LYM1044 barley|12v1|BF617642 300507 LYM1046 barley|12v1|BF621200 301 508 LYM1047 barley|12v1|BF623265302 509 LYM1049 barley|12v1|BF625372 303 511 LYM1054barley|12v1|BI949533 307 515 LYM1055 barley|12v1|BI949678 308 516LYM1056 barley|12v1|BI950318 309 517 LYM1057 barley|12v1|BI951064 310518 LYM1060 barley|12v1|BI953477 311 521 LYM1061 barley|12v1|BI953521312 522 LYM1062 barley|12v1|BI954539 313 523 LYM1063barley|12v1|BI955576 314 524 LYM1065 barley|12v1|BI956211 315 526LYM1066 barley|12v1|BJ448998 316 527 LYM1068 barley|12v1|BJ469847 317528 LYM1069 barley|12v1|BLYCPPHSYS 318 529 LYM1072 barley|12v1|BU991227321 532 LYM1073 barley|12v1|CA023266 322 533 LYM1074barley|12v1|CB875234 323 534 LYM1079 brachypodium|12v1|BRADI1G23900 326538 LYM1082 brachypodium|12v1|BRADI1G69180 327 539 LYM1084brachypodium|12v1|BRADI1G78710 328 540 LYM1087brachypodium|12v1|BRADI2G26280 331 543 LYM1088brachypodium|12v1|BRADI2G41510T2 332 544 LYM1089brachypodium|12v1|BRADI2G51480 333 545 LYM1092brachypodium|12v1|BRADI3G45820 335 548 LYM1093brachypodium|12v1|BRADI3G55550 336 549 LYM1095brachypodium|12v1|BRADI4G29320 338 551 LYM1096brachypodium|12v1|BRADI4G29780 339 552 LYM1097brachypodium|12v1|BRADI4G31900T2 340 553 LYM1099brachypodium|12v1|BRADI5G25760 341 555 LYM1100foxtail_millet|11v3|EC612472 342 556 LYM1102foxtail_millet|11v3|GT091058 343 558 LYM1103foxtail_millet|11v3|PHY7SI000771M 344 559 LYM1104foxtail_millet|11v3|PHY7SI001398M 345 560 LYM1106foxtail_millet|11v3|PHY7SI003001M 346 562 LYM1107foxtail_millet|11v3|PHY7SI006370M 347 563 LYM1108foxtail_millet|11v3|PHY7SI006690M 348 564 LYM1109foxtail_millet|11v3|PHY7SI010388M 349 565 LYM1110foxtail_millet|11v3|PHY7SI010853M 350 566 LYM1112foxtail_millet|11v3|PHY7SI016985M 352 568 LYM1113foxtail_millet|11v3|PHY7SI017913M 353 569 LYM1114foxtail_millet|11v3|PHY7SI020310M 354 570 LYM1115foxtail_millet|11v3|PHY7SI020693M 355 571 LYM1116foxtail_millet|11v3|PHY7SI020984M 356 572 LYM1117foxtail_millet|11v3|PHY7SI023427M 357 573 LYM1118foxtail_millet|11v3|PHY7SI026065M 358 574 LYM1120foxtail_millet|11v3|PHY7SI030030M 359 576 LYM1121foxtail_millet|11v3|PHY7SI033845M 360 577 LYM1122foxtail_millet|11v3|PHY7SI033950M 361 578 LYM1124foxtail_millet|11v3|PHY7SI034927M 363 580 LYM1126foxtail_millet|11v3|PHY7SI036407M 365 582 LYM1128foxtail_millet|11v3|PHY7SI039767M 367 584 LYM1129 maize|10v1|AA054811368 585 LYM1132 maize|10v1|AI396444 371 588 LYM1133 maize|10v1|AI600333372 589 LYM1134 maize|10v1|AI621954 373 590 LYM1136 maize|10v1|AI666204374 591 LYM1137 maize|10v1|AI770430 375 592 LYM1138 maize|10v1|AI770478376 593 LYM1139 maize|10v1|AI855153 377 594 LYM1140 maize|10v1|AI901707378 595 LYM1141 maize|10v1|AI920541 379 596 LYM1146 maize|10v1|AW017808382 599 LYM1149 maize|10v1|AW171826 383 600 LYM1151 maize|10v1|AW313252384 601 LYM1152 maize|10v1|AW438140 385 602 LYM1153 maize|10v1|AW498006386 603 LYM1154 maize|10v1|AW499246 387 604 LYM1155 maize|10v1|AW566480388 605 LYM1156 maize|10v1|AW600668 389 606 LYM1157 maize|10v1|BE056858390 607 LYM1159 maize|10v1|BE640016 392 609 LYM1160 maize|10v1|BG320172393 610 LYM1161 maize|10v1|BG320248 394 611 LYM1163 maize|10v1|BG462317395 613 LYM1165 maize|10v1|BG836953 396 615 LYM1169 maize|10v1|BM336987399 618 LYM1170 maize|10v1|BM379348 400 619 LYM1172 maize|10v1|BQ538346402 621 LYM1174 maize|10v1|CD433365 404 623 LYM1175 maize|10v1|CD651832405 624 LYM1178 maize|10v1|CF028527 408 627 LYM1179 maize|10v1|CF046250409 628 LYM1180 maize|10v1|CF244711 410 629 LYM1181 maize|10v1|T15274411 630 LYM1182 maize|10v1|T20342 412 631 LYM1183 maize|10v1|T20362 413632 LYM1188 rice|11v1|AU065865 418 637 LYM1189 rice|11v1|AU093411 419638 LYM1190 rice|11v1|AU172407 420 639 LYM1191 rice|11v1|BI805353 421640 LYM1192 rice|11v1|BI806487 422 641 LYM1193 rice|11v1|BI812921 423642 LYM1194 rice|11v1|CA762027 424 643 LYM1195 sorghum|12v1|AW677825 425644 LYM1201 sorghum|12v1|SB01G005360 426 645 LYM1202sorghum|12v1|SB01G006060 427 646 LYM1206 sorghum|12v1|SB02G028130 431650 LYM1207 sorghum|12v1|SB03G011260 432 651 LYM1208sorghum|12v1|SB03G012430 433 652 LYM1209 sorghum|12v1|SB03G043920 434653 LYM1210 sorghum|12v1|SB06G030260 435 654 LYM1211sorghum|12v1|SB09G006520 436 655 LYM1212 sorghum|12v1|SB09G019550 437656 LYM1213 sorghum|12v1|SB09G026150 438 657 LYM1214sorghum|12v1|SB10G006840 439 658 LYM1215 sorghum|13v2|JGIV2SB13002974440 659 LYM1216 soybean|11v1|GLYMA03G35460 441 660 LYM1217soybean|11v1|GLYMA06G11380 442 661 LYM1218 soybean|11v1|GLYMA07G03580443 662 LYM1219 soybean|11v1|GLYMA08G19950 444 663 LYM1220soybean|11v1|GLYMA09G32550 445 664 LYM1221 soybean|11v1|GLYMA13G09620446 665 LYM1223 soybean|11v1|GLYMA13G21640 447 667 LYM1224soybean|11v1|GLYMA13G39770 448 668 LYM1225 soybean|11v1|GLYMA18G52430449 669 LYM1226 soybean|11v1|GLYMA19G28920 450 670 LYM1227soybean|11v1|GLYMA20G36230 451 671 LYM1228 tomato|11v1|AA824906 452 672LYM1229 tomato|11v1|AF153277 453 673 LYM1230 tomato|11v1|AW034456 454674 LYM1232 tomato|11v1|BG136313 456 676 LYM1233 tomato|11v1|BG734868457 677 LYM1234 barley|12v1|BE420804 458 678 LYM1235brachypodium|12v1|BRADI1G15290 459 679 LYM1239 sorghum|12v1|SB01G004200462 682 LYM1058_H4 maize|10v1|AI649819 465 685 LYM1059_H7maize|10v1|AI977980 466 686 LYM1064_H5 rice|13v2|BE228289 467 687LYM1076_H4 rice|11v1|CA755245 468 688 LYM1091_H5 maize|10v1|AW056217 469689 LYM1101_H3 sorghum|12v1|SB09G025690 470 690 LYM1105_H2sorghum|12v1|SB03G036770 471 691 LYM1013 barley|10v2|BE601902 272 715LYM1018 barley|12v1|AJ462240 277 716 LYM1020 barley|12v1|AJ465036 279717 LYM1022 barley|12v1|AJ476944 281 718 LYM1023 barley|12v1|AV832509282 719 LYM1030 barley|12v1|AV932314 289 720 LYM1034barley|12v1|BE412462 291 721 LYM1051 barley|12v1|BG309076 304 722LYM1052 barley|12v1|BG414431 305 723 LYM1053 barley|12v1|BI947802 306724 LYM1070 barley|12v1|BQ465115 319 725 LYM1071 barley|12v1|BU990128320 726 LYM1075 barley|12v1|CX627609 324 727 LYM1078brachypodium|12v1|BRADI1G20385 325 728 LYM1085brachypodium|12v1|BRADI2G15900 329 729 LYM1086brachypodium|12v1|BRADI2G21837 330 730 LYM1090brachypodium|12v1|BRADI2G56310 334 731 LYM1094brachypodium|12v1|BRADI4G01140 337 732 LYM1111foxtail_millet|11v3|PHY7SI014287M 351 733 LYM1123foxtail_millet|11v3|PHY7SI034443M 362 734 LYM1125foxtail_millet|11v3|PHY7SI036157M 364 735 LYM1127foxtail_millet|11v3|PHY7SI037176M 366 736 LYM1130 maize|10v1|AA979759369 737 LYM1131 maize|10v1|AI001351 370 738 LYM1142 maize|10v1|AI941641380 739 LYM1143 maize|10v1|AI947474 381 740 LYM1158 maize|10v1|BE511433391 741 LYM1167 maize|10v1|BM073386 397 742 LYM1168 maize|10v1|BM074116398 743 LYM1171 maize|10v1|BM498926 401 744 LYM1173 maize|10v1|CA401866403 745 LYM1176 maize|10v1|CD943536 406 746 LYM1177 maize|10v1|CD964979407 747 LYM1184 maize|gb170|AI586584 414 748 LYM1185maize|gb170|AI967089 415 749 LYM1186 maize|gb170|AW267345 416 750LYM1187 maize|gb170|CD997839 417 751 LYM1203 sorghum|12v1|SB01G023320428 752 LYM1204 sorghum|12v1|SB01G046820 429 753 LYM1205sorghum|12v1|SB02G005630 430 754 LYM1231 tomato|11v1|AW617278 455 755LYM1236 brachypodium|12v1|BRADI5G10880 460 756 LYM1237brachypodium|12v1|BRADI5G10920 461 757 LYM1240 tomato|11v1|AW154852 463758 LYM1032_H1 wheat|12v3|CA635514 464 759 LYM1164_H1sorghum|12v1|SB02G009120 472 760 LYM1196 sorghum|12v1|CF480531 473 —Table 1: Provided are the identified genes, their annotation, organismand polynucleotide and polypeptide sequence identifiers. “polyn.” =polynucleotide; “polyp.” = polypeptide. Core genes SEQ ID NOs: 1-220(polynucleotides) and 474-692 (polypeptides); variants and curatedsequences SEQ ID NOs: 221-268 (polynucleotides); cloned sequences SEQ IDNOs: 269-473 (polynucleotides).

Example 2 Identification of Homologous Sequences that Increase Yield,Fiber Yield, Fiber Quality, Growth Rate, Biomass, PhotosyntheticCapacity Oil Content, Vigor, ABST, and/or NUE of a Plant

The concepts of orthology and paralogy have recently been applied tofunctional characterizations and classifications on the scale ofwhole-genome comparisons. Orthologs and paralogs constitute two majortypes of homologs: The first evolved from a common ancestor byspecialization, and the latter are related by duplication events. It isassumed that paralogs arising from ancient duplication events are likelyto have diverged in function while true orthologs are more likely toretain identical function over evolutionary time.

To further investigate and identify putative orthologs of the genesaffecting plant yield, oil yield, oil content, seed yield, growth rate,photosynthetic capacity, vigor, biomass, abiotic stress tolerance, andfertilizer use efficiency (FUE) genes and/or nitrogen use efficiency,all sequences were aligned using the BLAST (Basic Local Alignment SearchTool). Sequences sufficiently similar were tentatively grouped. Theseputative orthologs were further organized under a Phylogram—a branchingdiagram (tree) assumed to be a representation of the evolutionaryrelationships among the biological taxa. Putative ortholog groups wereanalyzed as to their agreement with the phylogram and in cases ofdisagreements these ortholog groups were broken accordingly.

Expression data was analyzed and the EST libraries were classified usinga fixed vocabulary of custom terms such as developmental stages (e.g.,genes showing similar expression profile through development with upregulation at specific stage, such as at the seed filling stage) and/orplant organ (e.g., genes showing similar expression profile across theirorgans with up regulation at specific organs such as seed). Theannotations from all the ESTs clustered to a gene were analyzedstatistically by comparing their frequency in the cluster versus theirabundance in the database, allowing the construction of a numeric andgraphic expression profile of that gene, which is termed “digitalexpression”. The rationale of using these two complementary methods withmethods of phenotypic association studies of QTLs, SNPs and phenotypeexpression correlation is based on the assumption that true orthologsare likely to retain identical function over evolutionary time. Thesemethods provide different sets of indications on function similaritiesbetween two homologous genes, similarities in the sequence level-identical amino acids in the protein domains and similarity inexpression profiles.

The search and identification of homologous genes involves the screeningof sequence information available, for example, in public databases suchas the DNA Database of Japan (DDBJ). Genbank, and the European MolecularBiology Laboratory Nucleic Acid Sequence Database (EMBL) or versionsthereof or the MIPS database. A number of different search algorithmshave been developed, including but not limited to the suite of programsreferred to as BLAST programs. There are five implementations of BLAST,three designed for nucleotide sequence queries (BLASTN, BLASTX, andTBLASTX) and two designed for protein sequence queries (BLASTP andTBLASTN) (Coulson. Trends in Biotechnology: 76-80, 1994; Birren et al.,Genome Analysis. I: 543, 1997). Such methods involve alignment andcomparison of sequences. The BLAST algorithm calculates percent sequenceidentity and performs a statistical analysis of the similarity betweenthe two sequences. The software for performing BLAST analysis ispublicly available through the National Centre for BiotechnologyInformation. Other such software or algorithms are GAP, BESTFIT, FASTAand TFASTA. GAP uses the algorithm of Needleman and Wunsch (J. Mol.Biol. 48: 443-453, 1970) to find the alignment of two complete sequencesthat maximizes the number of matches and minimizes the number of gaps.

The homologous genes may belong to the same gene family. The analysis ofa gene family may be carried out using sequence similarity analysis. Toperform this analysis one may use standard programs for multiplealignments e.g. Clustal W. A neighbour-joining tree of the proteinshomologous to the genes in this invention may be used to provide anoverview of structural and ancestral relationships. Sequence identitymay be calculated using an alignment program as described above. It isexpected that other plants will carry a similar functional gene(ortholog) or a family of similar genes and those genes will provide thesame preferred phenotype as the genes presented here. Advantageously,these family members may be useful in the methods of the invention.Example of other plants are included here but not limited to, barley(Hordeum vulgare), Arabidopsis (Arabidopsis thaliana), maize (Zea mays),cotton (Gossypium), Oilseed rape (Brassica napus), Rice (Oryza sativa),Sugar cane (Saccharum officinarum), Sorghum (Sorghum bicolor), Soybean(Glycine max), Sunflower (Helianthus annuus), Tomato (Lycopersiconesculentum), Wheat (Triticum aestivum).

The above-mentioned analyses for sequence homology can be carried out ona full-length sequence, but may also be based on a comparison of certainregions such as conserved domains. The identification of such domains,would also be well within the realm of the person skilled in the art andwould involve, for example, a computer readable format of the nucleicacids of the present invention, the use of alignment software programsand the use of publicly available information on protein domains,conserved motifs and boxes. This information is available in the PRODOM(biochem (dot) ucl (dot) ac (dot) uk/bsm/dbbrowser/protocol/prodomry(dot) html), PIR (pir (dot) Georgetown (dot) edu/) or Pfam (sanger (dot)ac (dot) uk/Software/Pfam) database. Sequence analysis programs designedfor motif searching may be used for identification of fragments, regionsand conserved domains as mentioned above. Preferred computer programsinclude, but are not limited to, MEME SIGNALSCAN, and GENESCAN.

A person skilled in the art may use the homologous sequences providedherein to find similar sequences in other species and other organisms.Homologues of a protein encompass, peptides, oligopeptides,polypeptides, proteins and enzymes having amino acid substitutions,deletions and/or insertions relative to the unmodified protein inquestion and having similar biological and functional activity as theunmodified protein from which they are derived. To produce suchhomologues, amino acids of the protein may be replaced by other aminoacids having similar properties (conservative changes, such as similarhydrophobicity, hydrophilicity, antigenicity, propensity to form orbreak a-helical structures or 3-sheet structures). Conservativesubstitution tables are well known in the art (see for example Creighton(1984) Proteins. W.H. Freeman and Company). Homologues of a nucleic acidencompass nucleic acids having nucleotide substitutions, deletionsand/or insertions relative to the unmodified nucleic acid in questionand having similar biological and functional activity as the unmodifiednucleic acid from which they are derived.

Polynucleotides and polypeptides with significant homology to theidentified genes described in Table 1 (Example 1 above) were identifiedfrom the databases using BLAST software with the Blastp and tBlastnalgorithms as filters for the first stage, and the needle (EMBOSSpackage) or Frame+algorithm alignment for the second stage. Localidentity (Blast alignments) was defined with a very permissivecutoff—60% Identity on a span of 60% of the sequences lengths because itis used only as a filter for the global alignment stage. The defaultfiltering of the Blast package was not utilized (by setting theparameter “-F F”).

In the second stage, homologues were defined based on a global identityof at least 80% to the core gene polypeptide sequence.

Two distinct forms for finding the optimal global alignment for proteinor nucleotide sequences were used in this application:

1. Between two proteins (following the blastp filter):

EMBOSS-6.0.1 Needleman-Wunsch algorithm with the following modifiedparameters: gapopen=8 gapextend=2. The rest of the parameters wereunchanged from the default options described hereinabove.

2. Between a protein sequence and a nucleotide sequence (following thetblastn filter):

GenCore 6.0 OneModel application utilizing the Frame+algorithm with thefollowing parameters: model=frame+_p2n.model mode=qglobal-q=protein.sequence -db=nucleotide.sequence. The rest of the parametersare unchanged from the default options described hereinabove.

The query polypeptide sequences were SEQ ID NOs: 474-760 (which areencoded by the polynucleotides SEQ ID NOs:1-473, shown in Table 1 above)and the identified orthologous and homologous sequences having at least80% global sequence identity are provided in Table 2, below. Thesehomologous genes am expected to increase plant yield, harvest index,seed yield, oil yield, oil content, photosynthetic capacity, growthrate, fiber yield, fiber quality, biomass, vigor, ABST and/or NUE of aplant.

TABLE 2 Homologues (e.g., orthologues) of the identifiedgenes/polypeptides for increasing yield, harvest index, fiber yield,fiber quality, photosynthetic capacity, vigor, biomass, growth rate,abiotic stress tolerance, nitrogen use efficiency, water use efficiencyand fertilizer use efficiency of a plant P.P. Hom. % P.N. SEQ SEQ to SEQglobal ID NO: Organism/cluster name ID NO: ID NO: iden. Algor. 761wheat|12v3|CA499808 4806 474 97.7 globlastp 762 wheat|12v3|BE471040 4807474 97.4 globlastp 763 rye|12v1|DRR001012.10625XX1 4808 474 96.62globlastn 764 leymuslgb166|EG374745_P1 4809 474 96.2 globlastp 765brachypodium|12v1|BRADI1G24110_P1 4810 474 92.5 globlastp 766pat|11v1|CM917559_P1 4811 474 89.1 globlastp 767 rice|11v1|BE229056 4812474 81.7 globlastp 768 switchgrass|12v1|GD046483_P1 4813 474 81.2globlastp 769 switchgrass|12v1|FE625606_P1 4814 474 81.2 globlastp 770fescuelgb161|CK802813_P1 4815 474 81.1 globlastp 771maize|10v1|W21719_P1 4816 474 80.8 globlastp 772sorghum|12v1|SB07G001230 4817 474 80.8 globlastp 773switchgrass|gb167|FE625606 4818 474 80.8 globlastp 774wheat|12v3|BE429597 4819 475 94.8 globlastp 775rye|12v1|DRR001012.100511 4820 475 92.8 globlastp 776brachypodium|12v1|BRADI2G01570_P1 4821 475 90.5 globlastp 777rye|12v1|DRR001012.163009 4822 475 88.4 globlastp 778foxtail_millet|11v3|PHY7SI002189M_P1 4823 475 84.8 globlastp 779sorghum|12v1|SB03G007820 4824 475 83.9 globlastp 780millet|10v1|EVO454PM004211_P1 4825 475 83.6 globlastp 781switchgrass|12v1|FL889709_P1 4826 475 83.3 globlastp 782maize|10v1|AI855205_P1 4827 475 80.8 globlastp 783 wheat|12v3|BE4180794828 476 95 globlastp 784 wheat|12v3|BE429779 4829 476 94.6 globlastp785 pseudoroegneria|gb167|FF345940 4830 476 93.9 globlastp 786leymus|gb166|EG390364_P1 4831 476 92.8 globlastp 787rye|12v1|DRR001012.168093 4832 476 92.5 globlastp 788oat|11v1|CN818992_P1 4833 476 92.1 globlastp 789brachypodium|12v1|BRADI2G19260_P1 4834 476 89.3 globlastp 790foxtail_millet|11v3|PHY7SI030773M_P1 4835 476 81 globlastp 791rice|11v1|BI118706 4836 476 80.2 globlastp 792switchgrass|12v1|DN145241_T1 4837 476 80.14 globlastn 793brachypodium|12v1|BRADI4G43490_P1 4838 477 85.1 globlastp 794brachypodium|12v1|SRR031799.357353_P1 4839 477 80.6 globlastp 795pseudoroegneria|gb167|FF343086 4840 478 93.9 globlastp 796wheat|12v3|CA602363 4841 478 93.2 globlastp 797 leymus|gb166|EG390333_P14842 478 92.8 globlastp 798 rye|12v1|BF145224 4843 478 92.8 globlastp799 rye|12v1|DRR001012.851131 4844 478 89.1 globlastp 800oat|11v1|GO588158_P1 4845 478 84.6 globlastp 801brachypodium|12v1|BRADI5G16540_P1 4846 478 81.7 globlastp 802rye|12v1|DRR001012.15709 4847 479 99.5 globlastp 803 wheat|12v3|CA4852624848 479 99.3 globlastp 804 wheat|12v3|CA68254 4849 479 99.3 globlastp805 wheat|12v3|CA679154 4850 479 99.85 globlastn 806wheat|12v3|SRR0723321X228577D1 4851 479 98.8 globlastp 807oat|11v1|GO581612_P1 4852 479 96.1 globlastp 808wheat|12v3|SRR400820X1013688D1 4853 479 94.7 globlastp 809wheat|12v3|BJ232559 4854 479 92.4 globlastp 810brachypodium|12v1|BRADI2G50850_P1 4855 479 91.8 globlastp 811rice|11v1|AA1753241 4856 479 90.3 globlastp 812 maize|10v1|AI714639_P14857 479 89.2 globlastp 813 foxtail_millet|11v3|PHY7SI01397M_P1 4858 47988.8 globlastp 814 sorghum|12v1|SB03G035520 4859 479 88.7 globlastp 815switchgrass|12v1|FL970432_P1 4860 479 88.3 globlastp 816switchgrass|gb167|FL970432 4861 479 88.3 globlastp 817millet|10v1|EVO454PM046432_P1 4862 479 88.1 globlastp 818switchgrass|12v1|FL970431_P1 4863 479 87.9 globlastp 819barley|12v1|BQ464462_T1 4864 479 87.3 globlastn 820millet|10v1|EVO454PM000736_P1 4865 479 86.8 globlastp 821sorghum|12v1|SB03G035510 4866 479 85.7 globlastp 822maize|10v1|AW091497_P1 4867 479 84.9 globlastp 823switchgrass|gb167|DN150274 4868 479 84.4 globlastp 824oil_palm|11v1|EL694748_T1 4869 479 83.49 globlastn 825monkeyflower|10v1|GR041261 4870 479 82.99 globlastn 826monkeyflower|12v1|SRR037227.103684_T1 4870 479 82.99 globlastn 827phalaenopsis|11v1|CK857490_T1 4871 479 82.99 globlastn 828grape|11v1|GSVIVT01000140001_T1 4872 479 82.95 globlastn 829clementine|11v1|CB293265_T1 4873 479 82.76 globlastn 830cotton|11v1|CO073391_T1 4874 479 82.76 globlastn 831gossypium_raimondii|12v1|DW507026_T1 4874 479 82.76 globlastn 832tragopogon|10v1|SRR010104S0003654 4875 479 82.76 globlastn 833oil_palm|11v1|EL691912_T1 4876 479 82.57 globlastn 834cirsium|11v1|SRR346952.1002551_T1 4877 479 82.53 globlastn 835cirsium|11v1|SRR346952.1002760_T1 4878 479 82.53 globlastn 836euphorbia|11v1|SRR098678X103708_T1 4879 479 82.53 globlastn 837soybean|11v1|GLYMA17G17530 4880 479 82.53 globlastn 838soybean|12v1|GLYMA17G17530_T1 4880 479 82.53 globlastn 839banana|12v1|FF561873_P1 4881 479 82.3 globlastp 840flaveria|11v1|SRR149241.195133_T1 4882 479 82.3 globlastn 841lotus|09v1|AW720090_T1 4883 479 82.3 globlastn 842sunflower|12v1|EE626878 4884 479 82.3 globlastn 843valeriana|11v1|SRR099039X103437 4885 479 82.3 globlastn 844grape|11v1|GSVIVT01000137001_T1 4886 479 82.26 globlastn 845poplar|13v1|BI122805_T1 4887 479 82.26 globlastn 846phalaenopsis|11v1|SRR125771.1108919_T1 4888 479 82.11 globlastn 847ambrosia|11v1|SRR346935.119908_T1 4889 479 82.07 globlastn 848arnica|11v1|SRR099034X132803_T1 4890 479 82.07 globlastn 849artemisia|EY037648_T1 4891 479 82.07 globlastn 850kiwi|gb166|FG431561_T1 4892 479 82.07 globlastn 851sunflower|12v1|DY950884 4893 479 82.07 globlastn 852poplar|13v1|BI122805 4894 479 82.03 globlastn 853centaurea|11v1|SRR346938.10437_T1 4895 479 81.84 globlastn 854ambrosia|11v1|GW917919_T1 4896 479 81.84 globlastn 855amsonia|11v|SRR098688X106652_T1 4897 479 81.84 globlastn 856cannabis|12v1|SOLX00011991_T1 4898 479 81.84 globlastn 857cassava|09v1|JGICASSAVA227VALIDM1_T1 4899 479 81.84 globlastn 858flaveria|11v1|SRR149229.142682_T1 4900 479 81.84 globlastn 859sesame|12v1|BU669477 4901 479 81.84 globlastn 860solanum_phureja|09v1|SPHG12654 4902 479 81.84 globlastn 861tomato|11v1|BG126754 4903 479 81.84 globlastn 862centaurea|11v1|EH711693_T1 4904 479 81.84 globlastn 863centaurea|11v1|EH715630_T1 4905 479 81.61 globlastn 864chestnut|gb170|SRR006295S0010685_T1 4906 479 81.61 globlastn 865lettuce|12v1|DW067582_T1 4907 479 81.61 globlastn 866oak|10v1|CU656079_T1 4908 479 81.61 globlastn 867grape|11v1|GSVIVT01000135001_T1 4909 479 81.57 globlastn 868cirsium|11v1|SRR346952.103401_P1 4910 479 81.4 globlastp 869oil_palm|11v1|GH636063_P1 4911 479 81.4 globlastp 870flaveria|11v1|SRR149229.114389_P1 4912 479 81.3 globlastp 871castorbean|12v1|EE255507_T1 4913 479 81.15 globlastn 872centaurea|gb166|EH711693 4914 479 81.15 globlastn 873nasturtium|11v1|SRR032559.69640_T1 4915 479 81.15 globlastn 874phyla|11v2|SRR099035X104507_T1 4916 479 80.96 globlastn 875prunus_mume|13v1|BU046122_T1 4917 479 80.92 globlastn 876arabidopsis_lyrata|09v1|JGIAL024200_T1 4918 479 80.92 globlastn 877arabidopsis|10v1|AT4G37550_T1 4919 479 80.92 globlastn 878flaveria|11v1|SRR149229.100158_T1 4920 479 80.92 globlastn 879orobanche|10v1|SRR023189S0081920_T1 4921 479 80.92 globlastn 880prunus|10v1|BU046122 4922 479 80.92 globlastn 881strawberry|11v1|GT149889 4923 479 80.92 globlastn 882thellungiella_halophilum|11v1|EHJGI11015175 4924 479 80.92 globlastn 883tripterygium|11v1|SRR098677X100344 4925 479 80.92 globlastn 884triphysaria|10v1|EY165587 4926 479 80.78 globlastn 885olea|13v1|SRR014463X1545D1_T1 4927 479 80.69 globlastn 886eucalyptus|11v2|ES481447_T1 4928 479 80.69 globlastn 887fagopyrum|11v1|SRR063703X115411_T1 4929 479 80.69 globlastn 888fraxinus|11v1|SRR058827.100115_T1 4930 479 80.69 globlastn 889medicago|12v1|AI974567_T1 4931 479 80.69 globlastn 890vinca|11v1|SRR098690X122826 4932 479 80.69 globlastn 891bean|12v2|SRR001335.439347_T1 4933 479 80.55 globlastn 892bean|12v1|SRR001335.439347 4934 479 80.55 globlastn 893olea|13v1|SRR014463X33113D1_T1 4935 479 80.46 globlastn 894b_rapa|11v1|CX192056_T1 4936 479 80.46 globlastn 895cichorium|gb171|EH675254_T1 4937 479 80.46 globlastn 896pigeonpea|11v1|sRR054580X10637_T1 4938 479 80.46 globlastn 897cacao|10v1|CU496860_P1 4939 479 80.3 globlastp 898catharanthur|11v1|EG560157_T1 4940 479 80.23 globlastn 899fraxinus|11v1|SRR058827.112715_T1 4941 479 80.23 globlastn 900aquilegia|10v2|DR918379_T1 4942 479 80 globlastn 901 wheat|12v3|BE6046224943 480 97.6 globlastp 902 wheat|12v3|BJ304531 4944 480 95.2 globlastp903 brachypodium|12v1|BRADI2G46650_P1 4945 480 93.1 globlastp 904foxtail_millet|11v3|PHY7SI001146M_P1 4946 480 86.8 globlastp 905maize|10v1|AW573435_P1 4947 480 85.7 globlastp 906sorghum|12v1|SB03G044340 4948 480 85.7 globlastp 907 rice|11v1|BM4212434949 480 85.5 globlastp 908 switchgrass|gb167|FL69863 4950 480 85.5globlastp 909 switchgrass|12v1|FL860465_P1 4951 480 85.1 globlastp 910rice|11v1|CA762920 4952 480 84.2 globlastp 911 sugarcane|10v1|CA1206334953 480 83 globlastp 912 maize|10v1|CD528010_P1 4954 480 81.6 globlastp913 rye|12v1|DRR001012.223257 4955 481 96.3 globlastp 914wheat|12v3|CA719595 4956 481 96.3 globlastp 915 wheat|12v3|CA723603 4957481 96.3 globlastp 916 wheat|12v3|CJ828308 4958 481 95.3 globlastp 917fescue|gb161|DT689385_P1 4959 481 83.2 globlastp 918oat|11v1|GR322552_P1 4960 481 83.2 globlastp 919brachypodium|12v1|BRADI1G78480_P1 4961 483 94.1 globlastp 920rye|12v1|DRR001013.132893 4962 483 93.4 globlastp 921wheat|12v3|BE418676 4963 483 92.7 globlastp 922foxtail_millet|11v3|PHY7SI036404M_P1 4964 483 91.1 globlastp 923switchgrass|12v1|FL977110_P1 4965 483 90.1 globlastp 924sorghum|12v1|SB01G050510 4966 483 90.1 globlastp 925 rice|11v1|CB2113254967 483 89.44 globlastn 926 millet|10v1|EVO454PM170482_P1 4968 483 89.4globlastp 927 maize|10v1|BM075301_P1 4969 483 88.4 globlastp 928rye|12v1|DRR001012.14520 4970 483 84.7 globlastp 929rye|12v1|DRR001012.142059 4971 484 96.6 globlastp 930brachypodium|12v1|BRADI1G78480_P1 4972 484 90.4 globlastp 931sorghum|12v1|SB09G026970 4973 484 87.5 globlastp 932maize|10v1|AI941555_P1 4974 484 87 globlastp 933 rice|11v1|AU175084 4975484 87 globlastp 934 foxtail_millet|11v3|PHY7SI021450M_P1 4976 484 86.9globlastp 935 switchgrass|12v1|FE601175_P1 4977 484 86.6 globlastp 936switchgrass|gb167|FE601175 4978 484 86.4 globlastn 937wheat|12v3|BG263512 4979 484 84 globlastp 938 rye|12v1|DRR001012.1078564980 485 95.4 globlastp 939 wheat|12v3|CD939313 4981 485 95.1 globlastp940 leymus|gb166|EG376968_P1 4982 485 92.8 globlastp 941rye|12v1|DRR001012.156292 4983 488 86.83 globlastn 942rye|12v1|DRR001012.109545 4984 489 98.65 globlastn 943rye|12v1|DRR001012.188810 4985 489 98.6 globlastp 944wheat|12v3|BI751390 4986 489 98.3 globlastp 945rye|12v1|DRR001017.1135370 4987 489 97.97 globlastn 946brachypodium|12v1|BRADI3G34090_P1 4988 489 92.2 globlastp 947sorghum|12v1|SB01G028320 4989 489 89.9 globlastp 948maize|10v1|T14791_P1 4990 489 88.9 globlastp 949 sugarcane|10v1|CA1452534991 489 88.9 globlastp 950 switchgrass|gb167|DN142883 4992 489 88.2globlastp 951 foxtail_millet|11v3|PHY7SI039140M_T1 4993 489 88.18globlastn 952 switchgrass|12v1|DN142883_P1 4994 489 87.5 globlastp 953switchgrass|12v1|FE644344_P1 4995 489 87.5 globlastp 954switchgrass|gb167|FE644344 4995 489 87.5 globlastp 955switchgrass|12v1|FL734512_P1 4996 489 87.2 globlastp 956rice|11v1|AA752933 4997 489 86.49 globlastn 957lovegrass|gb167|EH185476_T1 4998 489 86.15 globlastn 958switchgrass|12v1|JG798954_P1 4999 489 86.1 globlastp 959wheat|12v3|BE398714 5000 490 89.6 globlastp 960rye|12v1|DRR001012.170848 5001 490 88.83 globlastn 961rye|12v1|DRR001012.197116 5002 490 80.6 globlastp 962rye|12v1|DRR001012.201741 5003 491 97 globlastp 963 wheat|12v3|CB3077005004 491 92.1 globlastp 964 brachypodium|12v1|BRADI2G08826_T1 5005 49187.19 globlastn 965 foxtail_millet|11v3|PHY7SI000654M_P1 5006 491 86.2globlastp 966 switchgrass|12v1|FL717129_P1 5007 491 86 globlastp 967rice|11v1|AA749673 5008 491 85.8 globlastp 968 maize|10v1|AI941513_P15009 491 85.6 globlastp 969 sorghum|12v1|SB03G009530 5010 491 85.02globlastn 970 wheat|12v3|BE442783 5011 492 96.9 globlastp 971wheat|12v3|CA655313 5012 492 96.9 globlastp 972 wheat|12v3|BE445444 5013492 96.4 globlastp 973 rye|12v1|DRR001012.125087 5014 492 95.8 globlastp974 rye|12v1|DRR001012.112923 5015 492 95.3 globlastp 975rye|12v1|DRR001012.119920 5016 492 94.3 globlastp 976oat|11v1|GR313287_P1 5017 492 86.5 globlastp 977brachypodium|12v1|BRADI1G74470T2_P1 5018 492 84.4 globlastp 978wheat|12v3|BM138140 5019 492 80.6 globlastp 979 rye|12v1|BE494195 5020493 93.3 globlastp 980 wheat|12v3|CV771141 5021 493 93.3 globlastp 981leymus|gb166|EG377531_P1 5022 493 86.4 globlastp 982brachypodium|12v1|BRADI3G60477_P1 5023 494 85.1 globlastp 983wheat|12v3|SRR043323X104913D1 5024 495 82.4 globlastp 984wheat|12v3|SRR400820X153277D1 5025 496 83.58 globlastn 985rye|12v1|DRR001012.487346 5026 496 95.92 globlastn 986rye|12v1|DRR001012.145383 5027 496 94.9 globlastn 987oat|11v1|SRR020741.10036_T1 5028 496 80.61 globlastn 988oat|11v1|GR322199_T1 5029 496 80.58 globlastn 989 wheat|12v3|BE5919635030 497 89.3 globlastp 990 rye|12v1|DRR001012.422030 5031 497 84.2globlastp 991 rye|12v1|DRR001012.48982 5032 499 84.1 globlastp 992rye|12v1|DRR001013.302579 5033 499 81 globlastp 993leymus|gb166|CN466083_P1 5034 500 89.6 globlastp 994oat|11v1|CN817359_P1 5035 500 88.9 globlastp 995 wheat|12v3|BM1359265036 500 88.4 globlastp 996 rye|12v1|DRR001012.123874 5037 500 85.6globlastp 997 wheat|12v3|BE500186 5038 500 85.6 globlastp 998wheat|12v3|CA608058 5039 500 85 globlastp 999pseudoroegneria|gb167|FF357563 5040 500 84.9 globlastp 1000rye|12v1|DRR001012.109036 5041 500 84.9 globlastp 1001wheat|12v3|BG904763 5042 500 84.2 globlastp 1002 barley|12v1|BF267122_T15043 500 82.39 globlastn 1003 pseudoroegneria|gb167|FF340148 501 501 100globlastn 1004 rye|12v1|BE495303 5044 501 99.5 globlastp 1005leymus|gb166|CD808585_P1 5045 501 99.1 globlastp 1006wheat|12v3|BE490039 5046 501 99.1 globlastp 1007 oat|11v1|CN819069_P15047 501 92.9 globlastp 1008 oat|11v1|GR317596_P1 5048 501 92.9globlastp 1009 brachypodium|12v1|BRADI3G56270_P1 5049 501 88 globlastp1010 rice|11v1|CB209869 5050 501 84.2 globlastp 1011 rice|11v1|AA7519125051 501 84.19 globlastn 1012 switchgrass|12v1|DN145682_T1 5052 50183.72 globlastn 1013 foxtail_millet|11v3|EC613604_P1 5053 501 83.6globlastp 1014 millet|10v1|CD724620_P1 5054 501 83.2 globlastp 1015switchgrass|12v1|DN145555_T1 5055 501 81.48 globlastn 1016maize|10v1|AI649499_T1 5056 501 80.56 globlastn 1017sorghum|12v1|SB01G039270 5057 501 80.1 globlastp 1018wheat|12v3|BE404363 5058 502 97.7 globlastp 1019leymus|gb166|EG374806_P1 5059 502 97.1 globlastp 1020rye|12v1|DRR001012.120122 5060 502 97.1 globlastp 1021oat|11v1|GR325303_P1 5061 502 91.7 globlastp 1022brachypodium|12v1|BRADI5G08820_P1 5062 502 91.6 Globlastp 1023rice|11v1|AA751818 5063 502 85.67 Globlastn 1024 maize|10v1|AI966987_P15064 502 84.2 Globlastp 1025 rice|11v1|OSCRP090660 5065 502 84.1Globlastp 1026 sorghum|12v1|SB01G015130 5066 502 83.2 Globlastp 1027switchgrass|12v1|FL860465_P1 5067 502 82.9 Globlastp 1028switchgrass|gb167|DN145555 5067 502 82.9 Globlastp 1029fescue|gb161|DT679565_P1 5068 502 82.8 Globlastp 1030rye|12v1|DRR001012.32271 5069 503 97.7 Globlastp 1031brachypodium|12v1|BRADI3G20430_P1 5070 503 89 Globlastp 1032wheat|12v3|BE497566 5071 504 95.4 Globlastp 1033rye|12v1|DRR001012.134437 5072 504 90.8 Globlastp 1034leymus|gb166|CN465858_T1 5073 504 88.99 Globlastn 1035barley|12v1|AV933428_P1 5074 504 84.9 Globlastp 1036 wheat|12v3|BE3994165075 505 96.3 Globlastp 1037 leymus|gb166|EG395011_P1 5076 505 95.1Globlastp 1038 rye|12v1|BG263885 5077 505 95.1 Globlastp 1039fescue|gb161|DT698771_P1 5078 505 87.9 Globlastp 1040brachypodium|12v1|BRADI3G58930_P1 5079 505 83.5 Globlastp 1041wheat|12v3|AL827317 5080 506 92.1 Globlastp 1042 wheat|12v3|BF4730645081 506 91.6 Globlastp 1043 rye|12v1|DRR001012.215178 5082 506 83.5Globlastp 1044 wheat|12v3|BE399655 5083 507 96 Globlastp 1045brachypodium|12v1|BRADI3G46360_P1 5084 507 88.4 Globlastp 1046wheat|12v3|BE425895 5085 508 95.8 Globlastp 1047pseudoroegneria|gb167|FF363929 5086 508 95.5 Globlastp 1048rye|12v1|DRR001012.165033 5087 508 86.8 Globlastp 1049wheat|12v3|BE405019 5088 509 86.4 Globlastp 1050pseudoroegneria|gb167|FF357680 511 511 100 Globlastp 1051rye|12v1|BE587317 511 511 100 Globlastp 1052 rye|12v1|DRR001012.134892511 511 100 Globlastp 1053 wheat|12v3|CA679261 5089 511 98.7 Globlastp1054 brachypodium|12v1|BRADI3G29610_P1 5090 511 90.7 Globlastp 1055brachypodium|12v1|BRADI3G47027_P1 5091 511 89.3 Globlastp 1056banana|12v1|FL660779_P1 5092 511 86.7 Globlastp 1057cirsium|11v1|SRR346952.108326_P1 5093 511 86.7 Globlastp 1058cyanra|gb167|GE581359_P1 5094 511 86.7 Globlastp 1059onion|12v1|SRR073446X115892D1_P1 5095 511 86.7 Globlastp 1060salvia|10v1|FE537265 5096 511 85.7 Globlastp 1061zostera|12v1|SRR057351X135209D1_T1 5097 511 85.33 glotblastn 1062centaurea|11v1|EH741216_P1 5098 511 85.3 Globlastp 1063centaurea|11v1|SRR346938.147584_P1 5098 511 85.3 Globlastp 1064cassava|09v1|DV441272_P1 5099 511 85.3 Globlastp 1065centaurea|gb166|EH741216 5098 511 85.3 Globlastp 1066cirsium|11v1|SRR346952.1098629_P1 5098 511 85.3 Globlastp 1067grape|11v1|GSVIVT01037255001_P1 5100 511 85.3 Globlastp 1068oat|11v1|SRR020741.138429_P1 5101 511 85.3 Globlastp 1069onion|12v1|SRR073446X108711D1_P1 5102 511 85.3 Globlastp 1070poplar|10v1|BU809023 5103 511 85.3 Globlastp 1071poplar|10v1|BU809023_P1 5103 511 85.3 Globlastp 1072salvia|10v1|CV167583 5104 511 85.3 Globlastp 1073senecio|gb170|SRR006592S0012274 5105 511 85.3 Globlastp 1074nicotianna_benthamiana|12v1|EB695973_P1 5106 511 84 Globlastp 1075castorbean|11v1|EG681425 5107 511 84 Globlastp 1076castorbean|11v1|EG681425_P1 5107 511 84 Globlastp 1077chickpea|11v1|GR408157 5108 511 84 Globlastp 1078chickpea|13v2|GR395617_P1 5108 511 84 Globlastp 1079cirsium|11v1|SRR346941.247032XX2_P1 5109 511 84 Globlastp 1080cotton|11v1|BF277946_P1 5110 511 84 Globlastp 1081cucurbita|11v1|SRR091276X103592_P1 5111 511 84 Globlastp 1082euonymus|11v1|SRR070038X178196_P1 5112 511 84 Globlastp 1083euphorbia|11v1|BP954160_P1 5110 511 84 Globlastp 1084euphorbia|11v1|DV116401_P1 5110 511 84 Globlastp 1085gossypium_raimondii|12v1|BF277946_P1 5110 511 84 Globlastp 1086maize|10v1|AI737075_P1 5113 511 84 Globlastp 1087 melon|10v1|AM715730_P15111 511 84 Globlastp 1088 momordica|10v1|SRR071315S0054114_P1 5111 51184 Globlastp 1089 phalaenopsis|11v1|sRR125771.1779447_P1 5114 511 84Globlastp 1090 pigeonpea|11v1|SRR054580X150846_P1 5115 511 84 Globlastp1091 plantago|11v2|SRR066373X101918_P1 5116 511 84 Globlastp 1092potato|10v1|CV494926_P1 5117 511 84 Globlastp 1093solanum_phureja|09V1|SPHAW618453 5117 511 84 Globlastp 1094sorghum|12v1|SB06G030830 5118 511 84 Globlastp 1095soybean|11v1|BF008960 5115 511 84 Globlastp 1096soybean|12v1|GLYMA18G14801_P1 5115 511 84 Globlastp 1097spurge|gb161|DV116401 5119 511 84 Globlastn 1098 tamarix|gb166|EG724925120 511 84 glotblastn 1099 thellungiella_halophilum|11v1|EHJGI110038935121 511 84 Globlastp 1100 thellungiella_halophilum|11v1|EHJGI110228285122 511 84 Globlastp 1101 tobacco|gb162|EB432772 5123 511 84 Globlastp1102 triphysaria|10v|EY010916 5124 511 84 Globlastp 1103tripterygium|11v1|SRR098677X192105 5112 511 84 Globlastp 1104watermelon|11v1|AM715730 5111 511 84 Globlastp 1105zostera|10v1|SRR057351S0135210 5125 511 83.1 Globlastp 1106monkeyflower|12v1|MGJGI014705_P1 5126 511 82.7 Globlastp 1107olea|13v1|SRR014463X24477D1_P1 5127 511 82.7 Globlastp 1108arabidopsis_lyrata|09v1|JGIAL009222_P1 5128 511 82.7 Globlastp 1109arnica|11v1|SRR099034X107477_P1 5129 511 82.7 Globlastp 1110blueberry|12v1|SRR353282X15243D1_P1 5130 511 82.7 Globlastp 1111bruguiera|gb166|BP943731_P1 5131 511 82.7 Globlastp 1112chestnut|gb170|SRR006295S0035613_P1 5132 511 82.7 Globlastp 1113cleome_gynandra|10v1|SRR015532S0004228_P1 5133 511 82.7 Globlastp 1114cleome_gynandra|10v1|SRR015531S0064405_P1 5134 511 82.7 Globlastp 1115dandelion|10v1|GO663576_P1 5129 511 82.7 Globlastp 1116eggplant|10v1|FS052665_P1 5135 511 82.7 Globlastp 1117euonymus|11v1|SRR070038X152626_P1 5136 511 82.7 Globlastp 1118fagopyrum|11v1|SRR063689X103716_P1 5137 511 82.7 Globlastp 1119fagopyrum|11v1|SRR063703X119295_P1 5137 511 82.7 Globlastp 1120flax|11v1|JG091613_P1 5130 511 82.7 Globlastp 1121fraxinus|11v1|FR644589_P1 5138 511 82.7 Globlastp 1122gossypium_raimondii|12v1|DW502606_P1 5139 511 82.7 Globlastp 1123hornbeam|12v1|SRR364455.120956_P1 5140 511 82.7 Globlastp 1124lettuce|12v1|DW071404_P1 5129 511 82.7 Globlastp 1125lotus|09v1|AV416961_P1 5141 511 82.7 Globlastp 1126medicago|12v1|AL380692_P1 5142 511 82.7 Globlastp 1127monkeyflower|10v1|DV207687 5126 511 82.7 Globlastp 1128nasturtium|11v1|GH164582_P1 5143 511 82.7 Globlastp 1129oak|10v1|FP01413_P1 5144 511 82.7 Globlastp 1130orobache|10v1|SRR023189S0030303_P1 5145 511 82.7 Globlastp 1131papaya|gb165|EX279093_P1 5133 511 82.7 Globlastp 1132plantago|11v2|SRR066373X112048_P1 5146 511 82.7 globlastp 1133poplar|10v1||BU874698 5147 511 82.7 Globlastp 1134poplar|13v1||BU874698_P1 5147 511 82.7 Globlastp 1135sarracenia|11v1|SRR192669.107084 5148 511 82.7 Globlastp 1136scabiosa|11v1|SRR063723X100953 5129 511 82.7 Globlastp 1137silene|11v1|GH291901 5149 511 82.7 Globlastp 1138 soybean|11v1|CA9356035150 511 82.7 Globlastp 1139 soybean|12v1|GLYMA08G41416_P1 5150 511 82.7Globlastp 1140 primula|11v1|SRR098679X19709_T1 5151 511 82.67 Glotblastn1141 ambrosia|11v1|SRR346943.121702_T1 5152 511 81.33 Glotblastn 1142cotton|11v1|SRR032878.248337_T1 5153 511 81.33 Glotblastn 1143ginseng|10v1|GR873365_T1 5154 511 81.33 Glotblastn 1144antirrhinum|gb166|AJ787817_P1 5155 511 81.3 Globlastp 1145beet|12v1|FG345630_P1 5156 511 81.3 Globlastp 1146cacao|10v1|CU569368_P1 5157 511 81.3 Globlastp 1147catharanthus|11v1|EG557286_P1 5158 511 81.3 Globlastp 1148chelidonium|11v1|SRR084752X8369_P1 5159 511 81.3 Globlastp 1149clementine|11v1|CK702142_P1 5160 511 81.3 Globlastp 1150cowpea|12v1|FF540483_P1 5161 511 81.3 Globlastp 1151curcuma|10v1|DY383002_P1 5162 511 81.3 Globlastp 1152epimedium|11v1|SRR013502.16811_P1 5163 511 81.3 Globlastp 1153flaveria|11v1|SRR149229.461290_P1 5164 511 81.3 Globlastp 1154flaveria|11v1|SRR149232.110155_P1 5164 511 81.3 Globlastp 1155flax|11v1|EU829659_P1 5165 511 81.3 Globlastp 1156 flax|11v1|JG089166_P15165 511 81.3 Globlastp 1157 humulus|11v1|EX520119_P1 5166 511 81.3Globlastp 1158 ipomoea_batatas|10v1|DC880518_P1 5167 511 81.3 Globlastp1159 maize|10v1|DC661850_P1 5168 511 81.3 Globlastp 1160oil_palm|11v1|CN601090_P1 5169 511 81.3 Globlastp 1161oil_palm|11v1SRR190698.136067XX1_P1 5170 511 81.3 Globlastp 1162orange|11v1|CK702142_P1 5160 511 81.3 Globlastp 1163phyla|11v2|SRR099035X101881_P1 5171 511 81.3 Globlastp 1164poplar|10v1||BU809431 5172 511 81.3 Globlastp 1165poplar|10v1||BU809431_P1 5172 511 81.3 Globlastp 1166switchgrass|12v1|FE600473_P1 5173 511 81.3 Globlastp 1167switchgrass|gb167|FE600473 5173 511 81.3 Globlastp 1168switchgrass|12v1|FL701472_P1 5173 511 81.3 Globlastp 1169switchgrass|gb167|FL701472 5173 511 81.3 Globlastp 1170vinca|11v1|SRR098690X375698 5174 511 81.3 Globlastp 1171artemisia|10v1|SRR019254S0003868_T1 5175 511 80.52 Glotblastn 1172amsonia|11v1|SRR098688X208040_P1 5176 511 80 Globlastp 1173b_rapa|11v1|CV433672_P1 5177 511 80 Globlastp 1174beech|11v1|SRR006294.13815_P1 5178 511 80 Globlastp 1175canola|11v1|CN726675_P1 5179 511 80 globlastp 1176cichorium|gb171|FL671379_T1 5180 511 80 Glotblastn 1177eucalyptus|11v2|SRR001658X14353_P1 5181 511 80 Globlastp 1178flaveria|11v1|SRR149229.202694_T1 5182 511 80 Glotblastn 1179foxtail_millet|11v3|PHY7SI039204M_P1 5183 511 80 Globlastp 1180pepper|12v1|CA517896_P1 5184 511 80 Globlastp 1181poppy|11v1|SRR030259.127410_P1 5185 511 80 Globlastp 1182potato|10v1|BE922508_P1 5186 511 80 Globlastp 1183sesame|12v1|SESI12V1376997 5187 511 80 Globlastp 1184solanum_phureja|09v1|SPHBG129993 5186 511 80 Globlastp 1185tabernaemontana|11v1|SRR098689X113920 5188 511 80 Glotblastn 1186tomato|11v1|BG129993 5186 511 80 Globlastp 1187valeriana|11v1|SRR099039X104290 5178 511 80 Globlastp 1188rye|12v1|DRR001012.108965 5190 512 87.6 Globlastp 1189rye|12v1|DRR001012.212453 5191 512 86.2 Glotblastn 1190rye|12v1|DRR001012.185511 5192 512 83.23 Glotblastn 1191rye|12v1|DRR001012.357671 5193 514 94.5 Globlastp 1192rye|12v1|DRR001017.1001245 5194 514 93.2 Globlastp 1193wheat|12v3|BI480402 5195 514 92.9 Globlastp 1194leymus|gb166|CD808617_P1 5196 514 92.6 Globlastp 1195brachypodium|12v1|BRADI5G16650_P1 5197 514 87.1 Globlastp 1196rye|12v1|DRR001012.224308 5198 515 95.9 Globlastp 1197wheat|12v3|BE500638 5199 515 95.1 Globlastp 1198 wheat|12v3|CA7124165199 515 95.1 Globlastp 1199 brachypodium|12v1|BRADI3G30240_P1 5200 51591.4 Globlastp 1200 switchgrass|12v1|FL727897_P1 5201 515 88.8 Globlastp1201 foxtail_millet|11v3|EC612469_P1 5202 515 88.1 Globlastp 1202millet|10v1|EVO45PM146186_P1 5203 515 86.9 Globlastp 1203switchgrass|gb167|FL727897 5204 515 86.57 Globlastn 1204rice|11v1|AA751692 5205 515 86.3 Globlastp 1205 sorghum|12v1|SB01G0177805206 515 86.2 Globlastp 1206 maize|10v1|BQ635767_P1 5207 515 84Globlastp 1207 switchgrass|12v1|JG795801_P1 5208 515 80.8 Globlastp 1208rye|12v1|BE705431 5209 516 92.2 Globlastp 1209 wheat|12v3|BE418793 5210516 90.8 Globlastp 1210 pseudoroegneria|gb167|FF365512 5211 516 87.4Globlastp 1211 oat|11v1|CN818599_P1 5212 516 80.6 Globlastp 1212pseudoroegneria|gb167|FF345820 5213 517 97 Globlastp 1213wheat|12v3|BG906143 5214 517 94 Globlastp 1214 rye|12v1|DRR001012.1233135215 517 93.2 Globlastp 1215 wheat|12v3|CD863564 5216 517 93.2 Globlastp1216 rye|12v1|DRR001012.232649 5217 517 91 Globlastp 1217brachypodium|12v1|BRADI5G16380_P1 5218 517 82.7 globlastp 1218oat|11v1|SRR020741.108378_P1 5219 517 82.7 Globlastp 1219wheat|12v3|AL818554 5220 518 94.9 Globlastp 1220rye|12v1|DRR001013.158969 5221 518 93.57 Glotblastn 1221brachypodium|12v1|BRADI1G10060_P1 5222 518 84.9 Globlastp 1222wheat|12v3|BE401071 5223 519 98.1 Globlastp 1223 oat|11v1|GR321425_P15224 519 94.3 Globlastp 1224 brachypodium|12v1|BRADI2G56960_P1 5225 51987.47 Globlastp 1225 foxtail_millet|11v3|PHY7SI002920M_P1 5226 519 82.9Globlastp 1226 rye|12v1|DRR001012.106997 5227 519 82.8 Globlastp 1227sorghum|12v1|SB03G041820 5228 519 82 Globlastp 1228switchgrass|12v1|DN148669_P1 5229 519 81.8 Globlastp 1229rice|11v1|BE228600 5230 519 80.8 Globlastp 1230 wheat|12v3|BE471009 5231520 98.7 Globlastp 1231 brachypodium|12v1|BRADI2G52920_P1 5232 520 93.6Globlastp 1232 rice|11v1|BM419199 5233 520 92.3 Globlastp 1233rye|12v1|DRR001012.242488 5234 520 92.3 Globlastp 1234switchgrass|12v1|GD020803_P1 5235 520 92.1 Globlastp 1235foxtail_millet|11v3PHY7SI000692M_P1 5236 520 91.9 Globlastp 1236sorghum|12v1|SB03G037610 5237 520 91.7 Globlastp 1237switchgrass|12v1|FL762530_P1 5238 520 91.5 Globlastp 1238rye|12v1|DRR001012.170719 5239 521 93.6 Globlastp 1239rye|12v1|DRR001012.332063 5240 521 92.7 Globlastp 1240wheat|12v3|BQ838669 5241 521 92.2 Globlastp 1241 wheat|12v3|BE6048415242 521 91.3 Globlastp 1242 wheat|12v3|CA499090 5243 521 91.27Glotblastn 1243 brachypodium|12v1|BRADI4G18990_P1 5244 521 80.9Globlastp 1244 wheat|12v3|BE398791 5245 522 91.39 Glotblastn 1245wheat|12v3|CA501681 5246 522 90.73 Glotblastn 1246rye|12v1|DRR001012.104069 5247 522 90.7 Globlastp 1247rye|12v1|DRR001012.201893 5248 522 87.8 Globlastp 1248oat|11v1|GO589461_T1 5249 523 94.23 Glotblastn 1249 wheat|12v3|BQ2423985250 523 93.4 Globlastp 1250 rye|12v1|DRR001012.144328 5251 523 91.2Globlastp 1251 switchgrass|12v1|FE647391_P1 5252 523 87.9 Globlastp 1252sorghum|12v1|SB02G038400 5253 523 87.1 Globlastp 1253cemcjris|gb166|EB660107_P1 5254 523 86 Globlastp 1254sugarcane|10v1|BU103051 5255 523 85.71 Glotblastn 1255maize|10v1|AI901319_P1 5256 523 85.7 Globlastp 1256brachypodium|12v1|BRADI1G22510T2_T1 5257 523 84.34 glotblastn 1257leymus|gb166|CD808969_P1 5258 524 94.2 Globlastp 1258 rye|12v1|BF1454035259 524 93.8 Globlastp 1259 wheat|12v3|BE497921 5260 524 93.8 Globlastp1260 wheat|12v3|BE499980 5261 524 93.4 Globlastp 1261pseudoroegneria|gb167|FF343777 5262 524 93.3 Globlastp 1262rye|12v1|DRR001012.120114 5263 524 93.3 Globlastp 1263wheat|12v3|CA659640 5264 524 93.3 Globlastp 1264 wheat|12v3|BE4003395265 524 92.9 Globlastp 1265 wheat|12v3SRR043326X33530D1 5266 524 90.91Globlastn 1266 oat|11v1|CN816389_P1 5267 524 88.8 Globlastp 1267oat|11v1|GR353761_T1 5268 524 88.8 Globlastp 1268 barley|12v|BF621527_P15269 524 87.5 Globlastp 1269 rye|12v1|DRR001012.110990 5270 524 87.5Globlastp 1270 rye|12v1|DRR001012.408028 5271 524 87.5 Globlastp 1270oat|11v1|CN820431_P1 5272 524 87.1 Globlastp 1272 oat|11v1|CN821230_P15273 524 87.1 Globlastp 1273 wheat|12v3|BE606497 5274 524 87.1 Globlastp1274 rye|12v1|DRR001012.189460 5275 524 86.7 Globlastp 1275wheat|12v3|CA647190 5276 524 86.7 Globlastp 1276rye|12v1|DRR001017.1023470 5276 524 86.67 Glotblastn 1277lolium|10v|AU249832_T1 5277 524 86.25 Glotblastn 1278oat|11v1|GR330569_T1 5278 524 86.25 Glotblastn 1279brachypodium|12v1|BRADI2G26040_P1 5279 524 86.2 Globlastp 1280oat|11v1|CN821538XX2_P1 5280 524 86.2 Globlastp 1280 rice|11v1|BM4193735281 524 83.8 Globlastp 1282 switchgrass|12v1|DN143316_P1 5282 524 81.7Globlastp 1283 switchgrass|gb167|DN143316 5282 524 81.7 Globlastp 1284switchgrass|gb167|DN140861 5283 524 80.4 Globlastp 1285brachypodium|12v1|BRADI2G26050_P1 5284 524 80 Globlastp 1286rye|12v1|DRR001012.109115 5285 525 97.9 Globlastp 1287wheat|12v3|BE405752 5286 525 95.1 Globlastp 1288b_rachypodium|12v1|BRADI1G66220_P1 5287 525 93.7 Globlastp 1289oat|11v1|CN817185_T1 5288 525 92.33 Glotblastn 1290switchgrass|12v1|DN141372_P1 5289 525 90.6 Globlastp 1291foxtail_millet|11v3|PHY7SI034514M_P1 5290 525 90.3 Globlastp 1292switchgrass|12v1|FL810949_P1 5291 525 90.1 Globlastp 1293maize|10v1|CD986980_P1 5292 525 88.9 Globlastp 1294maize|10v1|CD970103_P1 5293 525 88.2 Globlastp 1295sorghum|12v1|SB01G039040 5294 525 88.2 Globlastp 1296 rye|12v1|BE5872135295 525 82.9 Globlastp 1297 wheat|12v3|BE401045 5296 526 99.1 Globlastp1298 rye|12v1|BF146231 5297 526 98.4 globlastp 1299rye|12v1|DRR001012.131820 5298 526 98.3 Globlastp 1300wheat|12v3|CA610861 5299 526 98.27 Glotblastn 1301 rye|12v1|BE4956445300 526 97.1 Globlastp 1302 wheat|12v3|BU100178 5301 526 95.8 Globlastp1303 rye|12v1|DRR001012.100172 5302 526 90.1 Globlastp 1304brachypodium|12v1|BRADI1G34700_P1 5303 526 89 Globlastp 1305switchgrass|12v1|FL729730_P1 5304 526 85.9 Globlastp 1306switchgrass|12v1|FE607339_P1 5305 526 85.7 Globlastp 1307foxtail_millet|11v3|EC612518_P1 5306 526 85.6 Globlastp 1308millet|10v1|EVO454PM003124_P1 5307 526 85.3 Globlastp 1309rice|11v|AF177292 5308 526 85.2 Globlastp 1310 sorghum|12v1|SB10G0294005309 526 85.2 Globlastp 1311 maize|10v1|AI622260_P1 5310 526 85Globlastp 1312 maize|10v1|AW066502_P1 5311 526 83.4 Globlastp 1313switchgrass|gb167|FE607339 5312 526 83.1 Globlastp 1314sugarcane|10v1|CA074388 5313 526 80.21 Glotblastn 1315wheat|12v3|BE497581 5314 527 98.3 Globlastp 1316rye|12v1|DRR001013.119954 5315 527 93.2 Globlastp 1317pseudoroegneria|gb167|FF363407 5316 527 91.5 Globlastp 1318rye|12v1|DRR001012.195564 5317 527 87 Globlastp 1319oat|11v1|GR314800_P1 5318 527 84.7 Globlastp 1320 wheat|12v3|CA6590825319 528 86.9 Globlastp 1321 wheat|12v3|BE390087 5320 528 85.9 Globlastp1322 rye|12v1|DRR001012.133312 5321 528 85.1 Globlastp 1323cotton|11v1|BN360881_P1 529 529 100 Globlastp 1324leymus|gb166|CD808644_P1 5322 529 98.8 Globlastp 1325pseudoroegneria|gb167|FF343068 5322 529 98.5 Globlastp 1326fescue|gb161|DT682777_P1 5323 529 96.2 Globlastp 1327lolium|10v1|AU247068_P1 5323 529 96.2 Globlastp 1328lolium|10v1|AU248778_P1 5323 529 96.2 Globlastp 1329lolium|10v1|ES700148_P1 5323 529 96.2 Globlastp 1330oat|11v1|CN817547_P1 5324 529 96.2 Globlastp 1331 rye|12v1|BE495991 5325529 96.2 Globlastp 1332 wheat|12v3|BE213292 5326 529 94.7 Globlastp 1333wheat|12v3|BE489656 5326 529 94.7 Globlastp 1334 wheat|12v3|BE5914095327 529 94.7 Globlastp 1335 wheat|12v3|BF484348 5327 529 94.7 Globlastp1336 wheat|12v3|BG313200 5327 529 94.7 Globlastp 1337wheat|12v3|CA622060 5326 529 94.7 Globlastp 1338 wheat|12v3|BE4011775328 529 93.9 Globlastp 1339 wheat|12v3|BQ902651 5328 529 93.9 Globlastp1340 wheat|12v3|CA598071 5328 529 93.9 Globlastp 1341 rye|12v1|BE7045175329 529 92.4 Globlastp 1342 rice|11v|BE228794 5330 529 91 Globlastp1343 brachypodium|12v1|BRADI1G58350_P1 5331 529 89.6 globlastp 1344sorghum|12v1|SB02G002960 5332 529 87.2 Globlastp 1345sugarcane|10v1|BQ534985 5333 529 87.2 Globlastp 1346 wheat|12v3|AL8099775334 529 87.02 Glotblastn 1347 maize|10v1|T12694_P1 5335 529 85.3Globlastp 1348 lovegrass|gb167|DN480912_P1 5336 529 85 Globlastp 1349wheat|12v3|ERR125556X287344D1 5337 529 84.21 Glotblastn 1350switchgrass|12v1|DN145166_T1 5338 529 82.71 Glotblastn 1351cynodon|10v1|ES294536_P1 5339 529 82.1 Globlastp 1352switchgrass|gb167|DN145166 5340 529 81.8 Globlastp 1353switchgrass|gb167|FL733379 5340 529 81.8 Globlastp 1354rye|12v1|DRR001012.213569 5341 530 96.1 Globlastp 1355brachypodium|12v1|BRADI3G47827_P1 5342 530 86.7 Globlastp 1356wheat|12v3|CD871072 5343 532 97.7 Globlastp 1357 wheat|12v3|BF474530XX15344 532 97.5 Globlastp 1358 wheat|12v3|BE400686 5345 532 89.6 Globlastp1359 brachypodium|12v1|BRADI1G11010_P1 5346 532 87.8 Globlastp 1360rice|11v|AU085903 5347 532 84 Globlastp 1361switchgrass|12v1|FL993073_P1 5348 532 83.9 Globlastp 1362foxtail_millet|11v3|PHY7SI035418M_P1 5349 532 83.1 Globlastp 1363switchgrass|12v1|HO255276_P1 5350 532 82.9 Globlastp 1364sorghum|12v1|SB01G010740 5351 532 82.2 Globlastp 1365maize|10v1|GFXAF466646X7_P1 5352 532 80.6 Globlastp 1366millet|10v1|EVO454PM107234_P1 5353 532 80.3 Globlastp 1367rye|12v1|DRR001012.488024 5354 533 84.7 Globlastp 1368pseudoroegneria|gb167|FF343480 5355 534 95.7 Globlastp 1369wheat|12v3|BE427624 5356 534 95.1 Globlastp 1370brachypodium|12v1|BRADI4G07630_P1 5357 534 85.5 Globlastp 1371leymus|gb166|EG385219_P1 5358 534 83.7 Globlastp 1372wheat|12v3|BE497169 5359 536 91.8 Globlastp 1373brachypodium|12v1|BRADI1G29260_P1 5360 536 88.9 Globlastp 1374rye|12v1|DRR001012.198573 5361 536 88.2 Globlastp 1375maize|10v1|CD435598_P1 5362 536 82.8 Globlastp 1376switchgrass|12v1|FE648909_P1 5363 536 81.9 Globlastp 1377switchgrass|12v1|JG772911_P1 5364 536 81.9 Globlastp 1378sorghum|12v1|SB04G008410 5365 536 81.9 Globlastp 1379foxtail_millet|11v3|PHY7SI017733M_P1 5366 536 81.7 Globlastp 1380oat|11v1|CN816172_P1 5367 538 90.5 Globlastp 1381leymus|gb166|EG375497_P1 5368 538 88 Globlastp 1382rye|12v1|DRR001012.178125 5369 538 87.6 Globlastp 1383rye|12v1|DRR0801012.109571 5370 538 87.5 globlastp 1384wheat|12v3|BG906668 5371 538 87.3 Globlastp 1385 rice|11v|BI305275 5372538 85.4 Globlastp 1386 foxtail_millet|11v3|PHY7SI030372M_P1 5373 53884.6 Globlastp 1387 sugarcane|10v1|CA093845 5374 538 84.4 Globlastp 1388switchgrass|gb167|FL843236 5375 538 84.3 Globlastp 1389sorghum|12v1|SB02G037160 5376 538 83.7 Globlastp 1390switchgrass|gb167|FE599488 5377 538 83.4 Globlastp 1391switchgrass|12v1|FE599488_P1 5377 538 83.4 Globlastp 1392pseudoroegneria|gb167|FF360233 5378 538 80.92 Glotblastn 1393maize|10v1|AW018052_P1 5379 538 80.7 Globlastp 1394 oat|11v1|GR329271_P15380 539 88.7 Globlastp 1395 wheat|12v3|CA698473 5381 539 87.2 Globlastp1396 leymus|gb166|EG376445_P1 5382 540 82.4 Globlastp 1397barley|12v1|BI956005_P1 5383 540 81.7 Globlastp 1398 wheat|12v3|CA4860005384 540 81.3 Globlastp 1399 wheat|12v3|CJ730962 5385 540 81.3 Globlastp1400 rye|12v1|DRR001012.193999 5386 542 83.76 Glotblastn 1401wheat|12v3|CN011800 5387 542 83.76 Glotblastn 1402 wheat|12v3|BE4980365388 542 81.7 Globlastp 1403 fescue|fb161|DT679740_P1 5389 545 93.9Globlastp 1404 fescue|fb161|DT691334_P1 5390 545 93.9 Globlastp 1405lolium|10v1|AU246445_P1 5390 545 93.9 Globlastp 1406 rye|12v1|BE7054215391 545 93.9 Globlastp 1407 rye|12v1|DRR001012.255916 5391 545 93.9Glotblastp 1408 wheat|12v3ABE213321 5392 545 93.13 Glotblastn 1409wheat|12v3|CA485361 5393 545 93.13 Glotblastn 1410 rye|12v1|BE4941745394 545 93.1 Globlastp 1411 rye|12v1|BE704951 5394 545 93.1 Globlastp1412 rye|12v1|DRR001012.111099 5395 545 93.1 Globlastp 1413rye|12v1|DRR001012.177842 5395 545 93.1 Globlastp 1414wheat|12v3|BE425717 5394 545 93.1 Globlastp 1415leymus|gb166|CD808577_P1 5396 545 92.4 Globlastp 1416oat|11v1|CN818813_P1 5397 545 92.4 Globlastp 1417 oat|11v1|GR318139_P15397 545 92.4 Globlastp 1418 pseudoroegneria|gb167|FF350411 5398 54592.4 Globlastp 1419 wheat|12v3|BE213279 5399 545 92.4 Globlastp 1420wheat|12v3|BE490342 5400 545 92.4 Globlastp 1421 wheat|12v3|BQ1724325401 545 90.8 Globlastp 1422 foxtail_millet|11v3|PHY7SI003335M_P1 5402545 86.3 Globlastp 1423 switchgrass|12v1|DN147511_P1 5403 545 85.5Globlastp 1424 lovegrass|gb167|DN480881_P1 5404 545 84.7 Globlastp 1425sorghum|12v1|SB03G036090 5405 545 84.7 Globlastp 1426millet|10v1|EVO454PM000328_P1 5406 545 84.1 Globlastp 1427maize|10v1|AA979823_P1 5407 545 83.2 Globlastp 1428sugarcane|10v1|BQ533710 5408 545 83.2 globlastp 1429cynodon|10v1|ES292620_P1 5409 545 80.9 Globlastp 1430rye|12v1|DRR001012.203718 5410 546 82.1 Globlastp 1431barley|12v1|AK362455_P1 5411 546 82 Globlastp 1432rye|12v1|DRR001012.364636XX2 5412 547 91.5 Globlastp 1433wheat|12v3|BG906134 5413 547 85.3 Globlastp 1434sorghum|12v1|SB01G019390 5414 547 84.9 Globlastp 1435switchgrass|12v1|FL796929_P1 5415 547 84.2 Globlastp 1436foxtail_millet|11v3|PHY7SI035447M_P1 5416 547 83.6 Globlastp 1437maize|10v1|AW0162079_T1 5417 547 82.5 Globlastn 1438switchgrass|12v1|HO311456_T1 5418 547 82.22 Glotblastn 1439wheat|12v3|BE422609 5419 548 88.8 Globlastp 1440rye|12v1|DRR001012.103563 5420 548 87.9 Globlastp 1441switchgrass|12v1|FL743281_P1 5421 548 86.3 Globlastp 1442rice|11v1|BE228296 5422 548 85.1 Globlastp 1443switchgrass|gb167|FL690808 5423 548 84.76 Glotblastn 1444switchgrass|12v1|FL690808_P1 5424 548 84.3 Globlastp 1445sorghum|12v1|SB04G022720 5425 548 83.9 Globlastp 1446sugarcane|10v1|CA070574 5426 548 83.5 Globlastp 1447foxtail_millet|11v3|PHY7SI017131M_P1 5427 548 82.6 Globlastp 1448maize|10v1|AI396326_P1 5428 548 81.5 Globlastp 1449maize|10v1|AW181177_P1 5429 548 81 Globlastp 1450barley|12v1|AJ467829_P1 5430 549 85.7 Globlastp 1451rye|12v1|DRR001012.319825 5431 549 84.09 Glotblastn 1452rice|11v1AU070649 5432 549 81.5 Globlastp 1453switchgrass|12v1|FE634937_P1 5433 551 86.3 Globlastp 1454switchgrass|gb167|FE634937 5433 551 86.3 Globlastp 1455switchgrass|12v1|FL763870_P1 5434 551 84.8 Globlastp 1456switchgrass|gb167|FL763870 5435 551 84.3 Globlastp 1457oat|11v1|GR316278_P1 5436 551 83.3 Globlastp 1458foxtail_millet|11v3|PHY7SI031243M_P1 5437 551 82.7 globlastp 1459sorghum|12v1|SB02G023570 5438 551 82.7 globlastp 1460wheat|12v3|BQ171089_P1 5439 551 82.2 globlastp 1461 wheat|12v3|BQ171089— 551 82.2 globlastp 1462 wheat|12v3|CA661505 5440 551 82.18 glotblastn1463 foxtail_millet|11v3|SIPRD087399_T1 5441 551 81.77 glotblastn 1464maize|10v1|BM428903_P1 5442 551 81.5 globlastp 1465 maize|10v1|W21661_P15443 551 81.2 globlastp 1466 wheat|12v3|CA623670 5444 552 87.35glotblastn 1467 rye|12v1|DRR001012.157917 5445 552 86.62 glotblastn 1468barley|12v1|BE454565_T1 5446 552 86.38 glotblastn 1469leymus|gb166|CD809177_P1 5447 552 86.2 globlastp 1470oat|11v1|GR317195_P1 5448 552 86.2 globlastp 1471pseudoroegneria|gb167|FF347096 5449 552 85.92 glotblastn 1472wheat|12v3|CA623873 5450 552 83.8 globlastp 1473 rye|12v1|BE495785 5451553 95.7 globlastp 1474 rye|12v1|DRR001012.134593 5451 553 95.7globlastp 1475 rye|12v1|DRR001012.147680 5451 553 95.7 globlastp 1476wheat|12v3|BE422943 5451 553 95.7 globlastp 1477 oat|11v1|GO590320_P15452 553 92.3 globlastp 1478 foxtail_millet|11v3|PHY7SI031452M_P1 5453553 92.2 globlastp 1479 maize|10v1|W21718_P1 5454 553 90.8 globlastp1480 millet|10v1|EVO454PM017015_P1 5455 553 90.8 globlastp 1481sorghum|12v1|SB02G026370 5454 553 90.8 globlastp 1482sugarcane|10v1|CA128222 5454 553 90.8 globlastp 1483switchgrass|12v1|FL774162_P1 5456 553 90.8 globlastp 1484switchgrass|gb167|FL774162 5456 553 90.8 globlastp 1485switchgrass|12v1|FL795215_P1 5457 553 90.8 globlastp 1486switchgrass|gb167|FL795215 5457 553 90.8 globlastp 1487fescue|gb161|DT692951_T1 5458 553 91.14 glotblastn 1488cenchrus|gb166|EB658601_P1 5459 553 90.1 globlastp 1489rice|11v1|BE040870 5460 553 90.1 globlastp 1490lovegrass|gb167|EH186261_P1 5461 553 88.7 globlastp 1491onion|12v1|SRR073446X183856D1_P1 5462 553 83.1 globlastp 1492oil_palm|11v1|SRR190698.156092_P1 5463 553 82.3 globlastp 1493banan|12v1|MAGEN2012032091_P1 5464 553 81.6 globlastp 1494oil_palm|11v1|SRR190698.146249_P1 5465 553 81.6 globlastp 1495pineapple|10v1|CO731184_P1 5466 553 80.3 globlastp 1496wheat|12v3|CA688346 5467 554 89 globlastp 1497wheat|12v3|SRR400820X1243828D1 5468 554 88.34 glotblastn 1498brachypodium|12v1|BRADI3G28640_T1 5469 554 88.2 globlastp 1499foxtail_millet|11v3|PHY7SI033896M_P1 5470 554 87.8 globlastp 1500rye|12v1|DRR001012.210311 5471 554 86.4 globlastp 1501wheat|12v3|BQ842364 5472 554 86.4 globlastp 1502rye|12v1|DRR001012.134897 5473 554 85.4 globlastp 1503rice|11v1|CA762958 5474 554 83.3 globlastp 1504 rice|11v1|CF323676 5475554 82.9 globlastp 1505 wheat|12v3|BJ228061 5476 554 82.8 globlastp 1506sorghum|12v1|CN141557 5477 554 81.6 globlastp 1507foxtail_millet|11v3|PHY7SI033895M_P1 5478 554 81.5 globlastp 1508switchgrass|12v1|FL705693_P1 5479 554 81.1 globlastp 1509switchgrass|12v1|DN150555_P1 5480 554 81 globlastp 1510foxtail_millet|11v3|PHY7SI000061M_P1 5481 554 80.9 globlastp 1511maize|10v1|AI1973421_P1 5482 554 80.8 globlastp 1512sorghum|12v1|SB06G031310_P1 5483 554 80.7 globlastp 1513switchgrass|gb167|FL725227 5484 556 94.92 glotblastn 1514switchgrass|12v1|FL725227_P1 5485 556 94.9 globlastp 1515switchgrass|12v1|FL786219_P1 5486 556 94.1 globlastp 1516sorghum|12v1|SB02G011240 5487 556 93.9 globlastp 1517maize|10v1|AW191437_P1 5488 556 92.9 globlastp 1518 rice|11v1|BM0389905489 556 86.7 globlastp 1519 rye|12v1|DRR001012.113915 5490 556 85.9globlastp 1520 rye|12v1|DRR001012.149120 5491 556 85.9 globlastp 1521brachypodium|12v1|BRADI4G38070_P1 5492 556 85.4 globlastp 1522wheat|12v3|BE415805 5493 556 85 globlastp 1523switchgrass|12v1|FL885636_P1 5494 557 91.9 globlastp 1524sugarcane|10v1|CA093115 5495 557 88.5 globlastp 1525switchgrass|gb167|FL885636 5496 557 87.86 glotblastn 1526millet|10v1|EVO454PM001855_T1 5497 557 85.68 glotblastn 1527maize|10v1|AA979989_P1 5498 557 85 globlastp 1528sorghum|12v1|SB09G025680 5499 557 82.6 globlastp 1529brachypodium|12v1|BRADI2G19700_P1 5500 557 81.3 globlastp 1530rice|11v1|AA754000 5501 557 81.3 globlastp 1531pseudoroegneria|gb167|FF359838 5502 557 81 globlastp 1532wheat|12v3|BF478401 5503 557 81 globlastp 1533 oat|11v1|GO586759_P1 5504557 80.5 globlastp 1534 rye|12v1|BE587278 5505 557 80.4 globlastp 1535rye|12v1|DRR001012.711688 5506 557 80.1 globlastp 1536switchgrass|12v1|FL756755_P1 5507 558 81.7 globlastp 1537switchgrass|12v1|FL715104_P1 5508 558 81.4 globlastp 1538maize|10v1|EC877313_P1 5509 559 84.8 globlastp 1539sorghum|12v1|SB03G035270 5510 559 83.7 globlastp 1540switchgrass|gb167|FE629499 5511 560 92.22 glotblastn 1541sorghum|12v1|SB03G037090 5512 560 90.3 globlastp 1542maize|10v1|CD941687_P1 5513 560 89.7 globlastp 1543barley|12v1|BG299761_P1 5514 560 86 globlastp 1544rye|12v1|DRR001012.117815 5515 560 84.3 globlastp 1545brachypodium|12v1|BRADI2G52390_P1 5516 560 83 globlastp 1546maize|10v1|CD942894_P1 5517 560 82.2 globlastp 1547switchgrass|12v1|FL879437_P1 5518 561 87.6 globlastp 1548switchgrass|12v1|DN148904_P1 5519 561 84.6 globlastp 1549maize|10v1|BE344993_P1 5520 561 80.2 globlastp 1550switchgrass|12v1|DN140762_P1 5521 562 94.4 globlastp 1551millet|10v1|EVO454PM450141_P1 5522 562 92.9 globlastp 1552switchgrass|gb167|FL726353 5523 562 86.1 globlastp 1553maize|10v1|BM339029_P1 5524 562 85.1 globlastp 1554sorghum|12v1|SB03G027790 5525 562 82.9 globlastp 1555switchgrass|12v1|DN147735_P1 5526 564 92 globlastp 1556sugarcane|10v1|BQ532774 5527 564 89.4 globlastp 1557switchgrass|12v1|DN141369_T1 5528 564 89.01 glotblastn 1558sorghum|12v1|SB10G026060 5529 564 88.5 globlastp 1559maize|10v1|T12685_P1 5530 564 86.8 globlastp 1560millet|10v1|EVO454PM016904_P1 5531 565 95.2 globlastp 1561switchgrass|12v1|FL798747_P1 5532 565 93.3 globlastp 1562switchgrass|gb167|FL981656 5533 565 93.3 globlastp 1563sorghum|12v1|SB06G028830 5534 565 92 globlastp 1564maize|10v1|BM338514_P1 5535 565 90.7 globlastp 1565maize|10v1|BM895380_P1 5536 565 88.5 globlastp 1566brachypodium|12v1|BRADI5G22070_P1 5537 565 84.5 globlastp 1567rice|11v1|BI801437 5538 565 83.3 globlastp 1568 wheat|12v3|BE516240 5539565 81.2 globlastp 1569 rye|12v1|DRR001012.10091 5540 565 80.7 globlastp1570 barley|12v1|BQ760382_P1 5541 565 80.5 globlastp 1571sorghum|12v1|SB04G004190 5542 568 80.7 globlastp 1572millet|10v1|EVO454PM036906_P1 5543 569 87.9 globlastp 1573switchgrass|12v1|FE606715_P1 5544 569 84.5 globlastp 1574switchgrass|12v1|FE597648_P1 5545 569 83.9 globlastp 1575switchgrass|gb167|FE597648 5546 569 83.6 globlastp 1576millet|10v1|PMSLX0012767D1_P1 5547 570 81.6 globlastp 1577switchgrass|gb167|FE609828 5548 570 80.5 globlastp 1578switchgrass|12v1|FL784509_P1 5549 571 94 globlastp 1579maize|10v1|AW267378_P1 5550 571 92 globlastp 1580sorghum|12v1|SB02G000365 5551 571 91.7 globlastp 1581millet|10v1|EVO454PM010170_P1 5552 571 80.6 globlastp 1582wheat|12v3|BE405523 5553 571 80 globlastp 1583 sorghum|12v1|SB09G0006705554 572 94 globlastp 1584 maize|10v1|AI622036_P1 5555 572 92.8globlastp 1585 wheat|12v3|SRR043323X32016D1 5556 572 91.96 glotblastn1586 rice|11v1|GFXAC079022X1 5557 572 89.7 globlastp 1587brachypodium|12v1|BRADI2G39420_P1 5558 572 88.8 globlastp 1588rye|12v1|DRR001012.201180 5559 572 83.9 globlastp 1589foxtail_millet|11v3|PHY7SI000064M_P1 5560 572 82.9 globlastp 1590rice|11v1|BE229765 5561 572 82.7 globlastp 1591 sorghum|12v1|SB03G0470105562 572 82.6 globlastp 1592 wheat|12v3|BQ743986 5563 572 82.1 globlastp1593 barley|12v1|AV913031_P1 5564 572 81.8 globlastp 1594brachypodium|12v1|BRADI2G61777_P1 5565 572 81.6 globlastp 1595wheat|12v3|CA699178 5566 572 81.55 glotblastn 1596barley|12v1|AW983306_P1 5567 572 81.2 globlastp 1597rye|12v1|DRR001012.17645 5568 572 81.02 glotblastn 1598rye|12v1|DRR001012.111897 5569 572 80.7 globlastp 1599wheat|12v3|BE492986 5570 572 80.48 glotblastn 1600millet|10v1|EVO454PM121012_P1 5571 573 98.9 globlastp 1601switchgrass|gb167|FE620088 5572 573 98.9 globlastp 1602rice|11v1|BE040122 5573 573 94.9 globlastp 1603rye|12v1|DRR001012.127367 5574 573 92.6 globlastp 1604wheat|12v3|BQ579626 5574 573 92.6 globlastp 1605 barley|12v1|BE060282_P15575 573 92 globlastp 1606 oat|11v1|CN815187_P1 5576 573 92 globlastp1607 rice|11v1|CA764875 5577 573 91.4 globlastp 1608brachypodium|12v1|BRADI3G29110_P1 5578 573 90.3 globlastp 1609maize|10v1|AI491684_P1 5579 573 90.3 glotblastn 1610sorghum|12v1|SB06G014440 5580 573 89.71 globlastp 1611maize|10v1|AW520044_P1 5581 573 89.7 globlastp 1612brachypodium|12v1|BRADI4G07940_P1 5582 573 89.1 globlastp 1613oil_palm|11v1|EY402276_P1 5583 573 81.8 globlastp 1614aristolochia|10v1|SRR039082S0001137_P1 5584 573 81.2 globlastp 1615melon|10v1|AM714468_P1 5585 573 80.6 globlastp 1616cucumber|09v1|DN910761_P1 5586 574 80 globlastp 1617sorghum|12v1|SB05G005850 5587 574 88.2 globlastp 1618maize|10v1|AI987267_P1 5588 574 87.6 globlastp 1619millet|10v1|EVO454PM027597_P1 5589 574 81 globlastp 1620switchgrass|12v1|FL707832_P1 5590 574 80.4 globlastp 1621switchgrass|12v1|FL920522_P1 5591 575 86.9 globlastp 1622sorghum|12v1|SB02G002790 5592 575 83.1 globlastp 1623sorghum|12v1|SB02G044070 5593 576 92.5 globlastp 1624maize|10v1|BE345024_P1 5594 576 92 globlastp 1625millet|10v1|EVO454PM059615_P1 5595 576 88.9 globlastp 1626brachypodium|12v1|BRADI1G16500_P1 5596 576 85.4 globlastp 1627leymus|gb166|EG378503_P1 5597 576 83.7 globlastp 1628wheat|12v3|CABE414106 5598 576 82.3 globlastp 1629 rice|11v1|BM0383825599 576 81 globlastp 1630 switchgrass|12v1|FL696404_P1 5600 578 98.8globlastp 1631 rice|11v1|BE530957 5601 578 96.7 globlastp 1632brachypodium|12v1|BRADI1G13720_P1 5602 578 96.5 globlastp 1633maize|10v1|MZEORFP_P1 5603 578 96.5 globlastp 1634rye|12v1|DRR001012.122396 5604 578 96.34 glotblastn 1635sorghum|12v1|SB01G013470 5605 578 95.9 globlastp 1636rye|12v1|DRR001012.122796 5606 578 95 globlastp 1637grape|11v1|GSVIVT01020543001_P1 5607 578 90.3 globlastp 1638cassava|09v1|CK644520_T1 5608 578 89.67 glotblastn 1639poplar|13v1|AI163021_P1 5609 578 89.5 globlastp 1640cotton|11v1|CO084380_P1 5610 578 89.4 globlastp 1641eucalyptus|11v2|CD669178_P1 5611 578 89.4 globlastp 1642gossypium_raimondii|12v1|DN801910_P1 5610 578 89.4 globlastp 1643catorbean|12v1|EG680374_P1 5612 578 89.2 globlastp 1644pigeonpea|11v1|SRR054580X104115_P1 5613 578 89.2 globlastp 1645poplar|10v1|AI163021 5614 578 89.18 glotblastn 1646bean|12v2|CA0902221_P1 5615 578 89.1 globlastp 1647 bean|12v2|CA09022215615 578 89.1 globlastp 1648 clementine|11v1|CN188446_P1 5616 578 89.1globlastp 1649 monkeyflower|10v1|GFXAJ565937X1 5617 578 89.1 globlastp1650 monkeyflower|12v1|DV209472_P1 5617 578 89.1 globlastp 1651orange|11v1|CN188446_P1 5616 578 89.1 globlastp 1652petunia|gb171|DQ020641_P1 5618 578 89.1 globlastp 1653solanum_phureja|09v1|SHLEU28403 5619 578 89.1 globlastp 1654soybean|11v1|GLYMA09G07570 5620 578 89.1 globlastp 1655soybean|12v1|GLYMA09G07570_P1 5620 578 89.1 globlastp 1656watermelon|11v1|AM737723 5621 578 89.1 globlastp 1657valerianna|11v1|SRR099039X108628 5622 578 89.01 glotblastn 1658nicotianna_benthamianal|12v1|BP748941_P1 5623 578 89 globlastp 1659aquilegia|10v2|DR927211_P1 5624 578 89 globlastp 1660trigonella|11v1|SRR066194X145996 5625 578 89 glotblastn 1661amborella|12v3|AY699216_P1 5626 578 88.9 globlastp 1662chickpea|11v1|GR392965 5627 578 88.8 globlastp 1663chickpea|11v1|GR392965 5627 578 88.8 globlastp 1664soybean|11v1|GLYMA15G18790 5628 578 88.8 globlastp 1665soybean|12v1|GLYMA15G18790_P1 5628 578 88.8 globlastp 1666tomato|11v1|LEU28403 5629 578 88.8 globlastp 1667antirrhinum|gb166|AY566619_P1 5630 578 88.7 globlastp 1668cacao|10v1|CA798124_P1 5631 578 88.7 globlastp 1669thellungiella_parvulum|11v1|EPCRP030837 5632 578 88.7 globlastp 1670nicotiana_benthamiana|12v1|GFXAY596739X1_P1 5633 578 88.6 globlastp 1671maize|10v|AI947765_T1 5634 578 88.59 glotblastn 1672arnica|11v1|SRR099034X100378_P1 5635 578 88.5 globlastp 1673medicago|12v1|AW686511_P1 5636 578 88.5 globlastp 1674ambrosia|11v1|SRR346935.112170_T1 5637 578 88.45 glotblastn 1675prunus|10v1|CN579460 5638 578 88.4 globlastp 1676prunus_mume|13v1|DW342897_P1 5639 578 88.3 globlastp 1677canola|11v1|DY004560_P1 5640 578 88.3 globlastp 1678tabernaemontana|11v1|SRR098689X101385 5640 578 88.3 globlastp 1679thellungiella_halophilum|11v1|EHJGI11013611 5642 578 88.3 globlastp 1680bean|12v2|SRR090491.1037431_P1 5643 578 88.2 globlastp 1681b_rapa|11v1|BG544904_P1 5644 578 88.2 globlastp 1682apple|11v1|CN579460_P1 5645 578 88.1 globlastp 1683arabidopsis_lyrata|09v1|JGIAL025979_P1 5646 578 88.1 globlastp 1684amsonia|11v1|SRR098688X100594_P1 5647 578 88 globlastp 1685plantago|11v2|SRR066373X106645_P1 5648 578 88 globlastp 1686arabidopsis|10v1|AT4G21710_P1 5649 578 87.9 globlastp 1687apple|11v1|CN580488_T1 5650 578 87.89 glotblastn 1688strawberry|11v1|DY667228 5651 578 87.8 globlastp 1689cucumber|09v1|AM737723_T1 5652 578 87.71 glotblastn 1690canola|11v1|EV055759_P1 5653 578 87.7 globlastp 1691b_rapa|11v1|AT002075_P1 5654 578 87.3 globlastp 1692poppy|11v1|GFXDQ017122X1_P1 5655 578 87.2 globlastp 1693abies|11v2|SRR098676X108534_P1 5656 578 87.1 globlastp 1694cephalotaxus|11v1|AY699209_P1 5657 578 87 globlastp 1695euphorbia|11v1|DV124499_P1 5658 578 85.7 globlastp 1696ceratodon|10v1|SRR074890S0024567_P1 5659 578 85.4 globlastp 1697physcomitrella|10v1|FC414727_P1 5660 578 85.4 globlastp 1698petunia|gb171|DQ020638_P1 5661 578 85 globlastp 1699monkeyflower|12v1|GFXAJ558241X1_P1 5662 578 84.6 globlastp 1700oil_palm|11v1|GH635997_P1 5663 578 84.6 globlastp 1701tomota|11v1|GFXAJ565936X1 5664 578 84.4 globlastp 1702antirrhinum|gb166|AY566620_P1 5665 578 83.9 globlastp 1703solanum_phureja|09v1|SPHGFXAJ565936X1 5666 578 83.9 globlastp 1704tripterygium|11v1|SRR098677X104577 5667 578 83.7 glotblastn 1705b_rapa|11v1|E6ANDIZ02G1AVU_P1 5668 578 83.1 globlastp 1706liriodendron|gb166|GFXAY566615X1_T1 5669 578 82.54 glotblastn 1707vinca|11v1|SRR098690X103405 5670 578 82.2 glotblastn 1708rye|12v1|DRR001012.103405 5671 578 82 globlastp 1709 wheat|12v3|CA6944045671 578 82 globlastp 1710 switchgrass|gb167|FL691093 5672 579 91.7globlastp 1711 switchgrass|12v1|FL702388_P1 5673 579 91.5 globlastp 1712maize|10v1|AW600635_P1 5674 579 85.4 globlastp 1713millet|10v1|EVO454PM004565_P1 5675 579 82.5 globlastp 1714switchgrass|12v1|GD047127_P1 5676 580 95.8 globlastp 1715switchgrass|12v1|FE622371_P1 5677 580 95.6 globlastp 1716maize|10v1|AW498340_P1 5678 580 92.5 globlastp 1717millet|10v1|EVO454PM007673_P1 5679 580 91.4 globlastp 1718rice|11v1|AU172537 5680 580 88.4 globlastp 1719 barley|12v1|BI951707_P15681 580 87.3 globlastp 1720 rye|12v1|DRR001012.108541 5682 580 86.84glotblastn 1721 brachypodium|12v1|BRADI1G07140_P1 5683 580 86.4globlastp 1722 wheat|12v3|CA731703 5684 580 86 globlastp 1723wheat|12v3|CJ688448 5685 580 86 globlastp 1724 wheat|12v3|BE430680 5676580 85.7 globlastp 1725 wheat|12v3|SRR043326X61261D1 5687 580 82.4globlastp 1726 sorghum|12v1|SB01G001470 5688 581 80.82 glotblastn 1727switchgrass|gb167|FL740714 5689 582 90.37 glotblastn 1728sorghum|12v1|SB01G034230 5690 582 88.6 globlastp 1729maize|10v1|AW191774_P1 5691 582 87 globlastp 1730switchgrass|12v1|FL740714_P1 5692 582 83.3 globlastp 1731switchgrass|12v1|GD032085_P1 5693 584 93.6 globlastp 1732switchgrass|12v1|FL876127_P1 5694 584 92.8 globlastp 1733cenchrus|gb166|EB659901_P1 5695 584 90.8 globlastp 1734maize|10v1|AW308661_P1 5696 584 85.5 globlastp 1735sorghum|12v1|SB01G038830 5697 584 85.5 globlastp 1736switchgrass|gb167|FL876127 5698 584 84.7 globlastp 1737barley|12v1|AV917342_P1 5699 584 80 globlastp 1738sorghum|12v1|SB08G017000 5700 586 96.5 globlastp 1739switchgrass|gb167|FL772843 5701 586 88.9 globlastp 1740sugarcane|10v1|CA150053 5702 586 88.6 globlastp 1741millet|10v1|EVO454PM003727_P1 5703 586 88.3 globlastp 1742switchgrass|12v1|FL772843_P1 5704 586 88.2 globlastp 1743maize|10v1|CF015939_T1 — 586 81.69 glotblastn 1744lovegrass|gb167|EH189424_P1 5705 586 81 globlastp 1745foxtail_millet|11v3|EC611921_P1 5706 586 80.7 globlastp 1746cynodon|10v1|ES298101_P1 5707 586 80.3 globlastp 1747 rice|11v1|BU5723895708 586 80.27 glotblastn 1748 sorghum|12v1|SB09G029060 5709 587 80.2globlastp 1749 foxtail_millet|11v3|PHY7SI028803M_P1 5710 588 96.7globlastp 1750 rice|11v1|BI799345 5711 588 92.5 globlastp 1751switchgrass|12v1|DN149726_P1 5712 588 92.1 globlastp 1752barley|12v1|AJ461484_P1 5713 588 90.4 globlastp 1753switchgrass|gb167|DN149726 5714 588 90.2 globlastp 1754switchgrass|12v1|FE601563_P1 5715 588 87.3 globlastp 1755banana|12v1|FF558588_P1 5716 588 85 globlastp 1755banana|12v1|FF558588_P1 5716 669 83.6 globlastp 1756oil_palm|11v1|ES370569_P1 5717 558 84.4 globlastp 1756oil_palm|11v1|ES370569_P1 5717 669 82.4 globlastp 1757rye|12v1|DRR001012.119625 5718 588 84.3 globlastp 1758wheat|12v3|BE493469 5719 588 83.9 globlastp 1758 wheat|12v3|BE4934695719 669 80.6 globlastp 1759 eucalyptus|11v2|CT986059_P1 5720 588 83.8globlastp 1759 eucalyptus|11v2|CT986059_P1 5720 669 84.8 globlastp 1760rye|12v1|DRR001012.102824 5721 588 83.8 globlastp 1760rye|12v1|DRR001012.102824 5721 669 80.6 globlastp 1761brachypodium|12v1|BRADI5G08235_P1 5722 588 83.7 globlastp 1761brachypodium|12v1|BRADI5G08235_P1 5722 669 80.3 globlastp 1762sorghum|12v1|SB06G013930 5723 588 83.7 globlastp 1762sorghum|12v1|SB06G013930 5723 669 80.8 globlastp 1763oat|11v1|CN817802_T1 5724 588 83.68 glotblastn 1764castorbean|12v1|EG656511_P1 5725 588 83.6 globlastp 1764castorbean|12v1|EG656511_P1 5725 669 85.8 globlastp 1765foxtail_millet|11v3|PHY7SI009245M_P1 5726 588 83.6 globlastp 1765foxtail_millet|11v3|PHY7SI009245M_P1 5726 669 80.5 globlastp 1766sorghum|12v1|SB06G013940 5727 588 83.6 globlastp 1766sorghum|12v1|SB06G013940 5727 669 80.8 globlastp 1767amborella|12v3|CK761744_P1 5728 588 83.4 globlastp 1767amborella|12v3|CK761744_P1 5728 669 82.2 globlastp 1768brachypodium|12v1|BRADI5G08230_P1 5729 588 80 globlastp 1768brachypodium|12v1|BRADI5G08230_P1 5729 669 83.4 globlastp 1769maize|10v1|T18386_P1 5730 588 80.7 globlastp 1769 maize|10v1|T18386_P15730 669 80.7 globlastp 1770 cassava|09v1|CK643428_P1 5731 588 83.3globlastp 1770 cassava|09v1|CK643428_P1 5731 669 85.2 globlastp 1771maize|10v1|T18293_P1 5732 588 83.3 globlastp 1771 maize|10v1|T18293_P15732 669 80.2 globlastp 1772 rice|11v1|U38053 5733 588 83.3 globlastp1772 rice|11v1|U38053 5733 669 80.9 globlastp 1773banana|12v1DN239957_P1 5734 588 83.2 globlastp 1773banana|12v1|DN239957_P1 5734 669 80.9 globlastp 1774cassava|09v1|JGOCASSAVA6609VALIDM1_P1 5735 588 83.2 globlastp 1774cassava|09v1|JGOCASSAVA6609VALIDM1_P1 5735 669 85.2 globlastp 1775chelidonium|11v1|SRR084752X106414_P1 5736 588 83.2 globlastp 1775chelidonium|11v1|SRR084752X106414_P1 5736 669 82.1 globlastp 1776apple|11v1|CN490891_P1 5737 588 83.1 globlastp 1776apple|11v1|CN490891_P1 5737 669 84.6 globlastp 1777cucumber|09v1|BU791062_P1 5738 588 83.1 globlastp 1777cucumber|09v1|BU791062_P1 5738 669 84.9 globlastp 1778tripterygium|11v1|SRR098677X10105 5739 588 83 globlastp 1778tripterygium|11v1|SRR098677X10105 5739 669 84.4 globlastp 1779poppy|11v1|FE967956_T1 5740 588 82.94 glotblastn 1779poppy|11v1|FE967956_T1 5740 669 82.78 glotblastn 1780poppy|11v1|SRR030269.135207_T1 5741 588 82.94 glotblastn 1780poppy|11v1|SRR030269.135207_T1 5741 669 82.78 glotblastn 1781switchgrass|12v1|FE641776_P1 5742 588 82.9 globlastp 1782amsonia|11v1|SRR098688X100786_P1 5743 588 82.9 globlastp 1782amsonia|11v1|SRR098688X100786_P1 5743 669 85.4 globlastp 1783poppy|11v1|SRR030259.104847_P1 5744 588 82.9 globlastp 1783poppy|11v1|SRR030259.104847_P1 5744 669 82.7 globlastp 1784nicotiana_benthamiana|12v1|BP749265_P1 5745 588 82.8 globlastp 1784nicotiana_benthamiana|12v1|BP749265_P1 5745 669 82.2 globlastp 1785watermelon|11v1|AM732793 5746 588 82.78 glotblastn 1785watermelon|11v1|AM732793 5746 669 84.71 glotblastn 1786poplar|10v1|BU808955 5747 588 82.7 globlastp 1786 poplar|10v1|BU8089555747 669 85.1 globlastp 1787 poplar|10v1|BU808955_P1 5747 588 82.7globlastp 1787 poplar|10v1|BU808955_P1 5747 669 85.1 globlastp 1788orange|11v1|CK701952_T1 5748 588 82.63 glotblastn 1788orange|11v1|CK701952_T1 5748 669 85.69 glotblastn 1789clementine|11v1|CK701952_P1 5749 588 82.6 globlastp 1789clementine|11v1|CK701952_P1 5749 669 85.4 globlastp 1790flaveria|11v1|SRR149232.301988_T1 5750 588 82.57 glotblastn 1790flaveria|11v1|SRR149232.301988_T1 5750 669 84.81 glotblastn 1791lettuce|12v1|DW108243_P1 5751 588 82.5 globlastp 1791lettuce|12v1|DW108243_P1 5751 669 83.8 globlastp 1792silene|11v1|SRR096785X103511 5752 588 82.5 globlastp 1792silene|11v1|SRR096785X103511 5752 669 83.5 globlastp 1793flaveria|11v1|SRR149229.105537_T1 5753 588 82.49 glotblastn 1793flaveria|11v1|SRR149229.105537_T1 5753 669 84.34 glotblastn 1794centaurea|11v1|EH17944_P1 5754 588 82.4 globlastp 1794centaurea|11v1|EH17944_P1 5754 669 84.5 globlastp 1795aqualegia|10v2|DR916365_P1 5755 588 82.4 globlastp 1795aqualegia|10v2|DR916365_P1 5755 669 83.4 globlastp 1796podocarpus|10v1|SRR065014S0001540_P1 5756 588 82.4 globlastp 1796podocarpus|10v1|SRR065014S0001540_P1 5756 669 82.3 globlastp 1797cotton|11v1|AI726463_T1 5757 588 82.37 glotblastn 1797cotton|11v1|AI726463_T1 5757 669 84.62 glotblastn 1798cotton|11v1|BF278372_T1 5758 588 82.37 glotblastn 1798cotton|11v1|BF278372_T1 5758 669 85 glotblastn 1799aqualegia|10v2|DR925645_P1 5759 588 82.3 globlastp 1799aqualegia|10v2|DR925645_P1 5759 669 84 globlastp 1800b_rapa|11v1|CD836760_P1 5760 588 82.3 globlastp 1800b_rapa|11v1|CD836760_P1 5760 669 82 globlastp 1801cacao|10v1|CU481473_P1 5761 588 82.3 globlastp 1801cacao|10v1|CU481473_P1 5761 669 84.5 globlastp 1802cotton|11v1|CO103364_P1 5762 588 82.3 globlastp 1802cotton|11v1|CO103364_P1 5762 669 84.6 globlastp 1803gossypium_raimondii|12v1|AI726463_P1 5763 588 82.3 globlastp 1803gossypium_raimondii|12v1|AI726463_P1 5763 669 84.6 globlastp 1804prunus|10v1|CN490891 5764 588 82.3 globlastp 1804 prunus|10v1|CN4908915764 669 84.5 globlastp 1805 prunus_mume|13v1|CV050672_P1 5765 588 82.2globlastp 1805 prunus_mume|13v1|CV050672_P1 5765 669 84.5 globlastp 1806gossypium_raimondii|12v1|AI728825_P1 5766 588 82.2 globlastp 1806gossypium_raimondii|12v1|AI728825_P1 5766 669 84.4 globlastp 1807sequoia|10v1|sRR065044S0004548 5767 588 82.2 globlastp 1807sequoia|10v1|sRR065044S0004548 5767 669 81.1 globlastp 1808ambrosia|11v1|SRR346935.100861_T1 5768 588 82.17 glotblastn 1808ambrosia|11v1|SRR346935.100861_T1 5768 669 84.62 glotblastn 1809cotton|11v1|DW492306_P1 5769 588 82.1 globlastp 1809cotton|11v1|DW492306_P1 5769 669 84.3 globlastp 1810ambrosia|11v1|SRR346935.108934_T1 5770 588 82.08 glotblastn 1810ambrosia|11v1|SRR346935.108934_T1 5770 669 84.71 glotblastn 1811flaveria|11v1|SRR149229.144621_T1 5771 588 82.08 glotblastn 1811flaveria|11v1|SRR149229.144621_T1 5771 669 84.23 glotblastn 1812vinca|11v1|SRR149229.144621_T1 5772 588 82.01 glotblastn 1812vinca|11v1|SRR149229.144621_T1 5772 669 82.2 globlastp 1813euphorbia|11v1|DV123408_P1 5773 588 82 globlastp 1813euphorbia|11v1|DV123408_P1 5773 669 85.3 globlastp 1814poppy|11v1|SRR096789.102474_T1 5774 588 82 glotblastn 1814poppy|11v1|SRR096789.102474_T1 5774 669 81.63 glotblastn 1815tomato|11v1|BG627851 5775 588 82 globlastp 1815 tomato|11v1|BG6278515775 669 82.6 globlastp 1816 poplar|13v1|AJ298115_P1 5776 588 82globlastp 1816 poplar|13v1|AJ298115_P1 5776 669 84.8 globlastp 1817nicotiana_benthamiana|12v1|EB695946_P1 5777 588 81.9 globlastp 1817nicotiana_benthamiana|12v1|EB695946_P1 5777 669 83.4 globlastp 1818arabidopsis_lyrata|09v1|JGIAL018794_P1 5778 588 81.9 globlastp 1818arabidopsis_lyrata|09v1|JGIAL018794_P1 5778 669 82.6 globlastp 1819arabidopsis|10v1|AT3G55410_P1 5779 588 81.9 globlastp 1819arabidopsis|10v1|AT3G55410_P1 5779 669 82.7 globlastp 1820poplar|13v1|AJ298115 5780 588 81.9 globlastp 1820 poplar|13v1|AJ2981155780 669 84.8 globlastp 1821 vinca|11v1|SRR098690X10694 5781 588 81.9globlastp 1821 vinca|11v1|SRR098690X10694 5781 669 84.5 globlastp 1822sciadopitys|10v1|SRR065035S0026542 5782 588 81.85 glotblastn 1822sciadopitys|10v1|SRR065035S0026542 5782 669 82.07 glotblastn 1823poppy|11v1|FE965289_T1 5783 588 81.81 glotblastn 1823poppy|11v1|FE965289_T1 5783 669 81.15 glotblastn 1824nicotiana_benthamiana|12v1|EB681944_P1 5784 588 81.7 globlastp 1824nicotiana_benthamiana|12v1|EB681944_P1 5784 669 81.2 globlastp 1825poppy|11v1|SRR030259.121112_P1 5785 588 81.7 globlastp 1825poppy|11v1|SRR030259.121112_P1 5785 669 81.8 globlastp 1826silene|11v1|SRR096785X106583 5786 588 81.7 globlastp 1826silene|11v1|SRR096785X106583 5786 669 82.5 globlastp 1827solanum_phureja|09v1|SPHBG627851 5787 588 81.7 globlastp 1827solanum_phureja|09v1|SPHBG627851 5787 669 82 globlastp 1828valeriana|11v1|SRR099039X106755 5788 588 81.7 glotblastn 1828valeriana|11v1|SRR099039X106755 5788 669 83.37 glotblastn 1829valeriana|11v1|SRR099039X117744 5789 588 81.7 globlastp 1829valeriana|11v1|SRR099039X117744 5789 669 85.1 globlastp 1830monkeyflower|10v1|GR041459 5790 588 81.6 globlastp 1830monkeyflower|10v1|GR041459 5790 669 83.1 globlastp 1831monkeyflower|12v1|GR137919_P1 5790 588 81.6 globlastp 1831monkeyflower|12v1|GR137919_P1 5790 669 83.1 globlastp 1832thellungiella_parvulum|11v1|DN776865 5791 588 81.6 globlastp 1832thellungiella_parvulum|11v1|DN776865 5791 669 82.4 globlastp 1833b_rapa|11v1|BG543370_P1 5792 588 81.5 globlastp 1833b_rapa|11v1|BG543370_P1 5792 669 82.7 globlastp 1834cephalotaxus|11v1|SRR064395X109569_P1 5793 588 81.5 globlastp 1834cephalotaxus|11v1|SRR064395X109569_P1 5793 669 80.9 globlastp 1835monkeyflower|10v1|CV521029 5794 588 81.5 globlastp 1835monkeyflower|10v1|CV521029 5794 669 84.1 globlastp 1836monkeyflower|10v1|CV521029_P1 5794 588 81.5 globlastp 1836monkeyflower|10v1|CV521029_P1 5794 669 84.1 globlastp 1837sunflower|12v1|CX946769 5795 588 81.5 globlastp 1837cephalotaxus|11v1|SRR064395X109569_P1 5795 669 84.7 globlastp 1838amorphophallus|11v2|SRR089351X598842_T1 5796 588 81.43 glotblastn 1838amorphophallus|11v2|SRR089351X598842_T1 5796 669 80.9 glotblastn 1839zostera|10v1|SRR057351S0013123 5797 588 81.4 globlastp 1840zostera|12v1|SRR057351X103792D1_P1 5797 588 81.4 globlastp 1841arabidopsis|10v1|AT5G65750_P1 5798 588 81.4 globlastp 1841arabidopsis|10v1|AT5G65750_P1 5798 669 82.6 globlastp 1842phalaenopsis|11v1|SRR125771.1006355_T1 5799 588 81.31 glotblastn 1842arabidopsis|10v1|AT5G65750_P1 5799 699 80.9 glotblastn 1843wheat|12v3|CA642089 5800 588 81.3 globlastp 1844arabidopsis_lyrata|09v1|JGIAL031268_P1 5801 588 81.3 globlastp 1844arabidopsis_lyrata|09v1|JGIAL031268_P1 5801 669 82.3 globlastp 1845taxus|10v1|SRR032523S0007163 5802 588 81.21 glotblastn 1845taxus|10v1|SRR032523S0007163 5802 669 81.01 glotblastn 1846solanum_phureja|09v1|SPHAJ302131 5803 588 81.2 globlastp 1846solanum_phureja|09v1|SPHAJ302131 5803 669 81.7 globlastp 1847strawberry|11v1|DY670233 5804 588 81.2 globlastp 1847strawberry|11v1|DY670233 5804 669 83.9 globlastp 1848tomato|11v1|AJ302131 5805 588 81.19 glotblastn 1848 tomato|11v1|AJ3021315805 669 81.37 glotblastn 1849 amorphophallus|11v2|SRR089351X125 5806588 81.1 globlastp 1849 amorphophallus|11v2|SRR089351X125 5806 669 81.9globlastp 1850 arnica|11v1|SRR099034X104253_P1 5807 588 81.1 globlastp1850 arnica|11v1|SRR099034X104253_P1 5807 669 84.5 globlastp 1851canola|11v1|ES905247_P1 5808 588 81 globlastp 1851canola|11v1|ES905247_P1 5808 669 80.9 globlastp 1852clementine|11v1|CF834323_P1 5809 588 81 globlastp 1852clementine|11v1|CF834323_P1 5809 669 84.1 globlastp 1853orange|11v1|CF834323_P1 5810 588 81 globlastp 1853orange|11v1|CF834323_P1 5810 669 84.1 globlastp 1854canola|11v1|EV192510_T1 5811 588 80.81 glotblastn 1854canola|11v1|EV192510_T1 5811 669 80.68 glotblastn 1855b_rapa|11v1|DY024002_P1 5812 588 80.8 globlastp 1855b_rapa|11v1|DY02402_P1 5812 669 81 globlastp 1856canola|11v1|EE416237_P1 5813 588 80.8 globlastp 1856canola|11v1|EE416237_P1 5813 669 81.8 globlastp 1857pine|10v2|AA739591_P1 5814 588 80.8 globlastp 1857 pine|10v2|AA739591_P15814 669 80.9 globlastp 1858 prunus_mume|13v1|DW342624_T1 5815 588 80.61globlastp 1858 prunus_mume|13v1|DW342624_P1 5815 669 80.5 globlastp 1859abies|11v2|SRR098676X101909_P1 5816 588 80.6 globlastp 1859abies|11v2|SRR098676X101909_P1 5816 669 80.9 globlastp 1860thellungiella_halophilum|11v1|DN772746 5817 588 80.6 globlastp 1860thellungiella_halophilum|11v1|DN772746 5817 669 82.6 globlastp 1861triphysaria|10v1|BM357063 5818 588 80.51 glotblastn 1861triphysaria|10v1|BM357063 5818 669 83.35 glotblastn 1862pseudotsuga|10v1|SRR065119S0003046 5819 588 80.47 glotblastn 1862pseudotsuga|10v1|SRR065119S0003046 5819 669 81.19 glotblastn 1863canola|11v1|EE453879_P1 5820 588 80.2 globlastp 1864spruce|11v1|ES659322 5821 588 80.18 glotblastn 1864 spruce|11v1|ES6593225821 669 80.62 glotblastn 1865 thellungiella_parvulum|11v1|DN775560 5822588 80.16 glotblastn 1865 thellungiella_parvulum|11v1|DN775560 5822 66982.59 glotblastn 1866 canola|11v1|EE451765_P1 5823 588 80.1 globlastp1866 canola|11v1|EE451765_P1 5823 669 80.1 globlastp 1867lettuce|12v1|LS12v1CRP091489_P1 5824 588 80.1 globlastp 1867lettuce|12v1|LS12v1CRP091489_P1 5824 669 81.4 globlastp 1868switchgrass|12v1|DN1447560_T1 5825 588 80.04 glotblastn 1869foxtail_millet|11v3|PHY7SI002922M_P1 5826 589 97.6 globlastp 1870sugarcane|10v1|BQ533119 5827 589 96.7 globlastp 1871millet|10v1|EVO454PM024192_P1 5828 589 96.2 globlastp 1872sorghum|12v1|SB03G037480 5829 578 96.2 globlastp 1873switchgrass|12v1|DN1506389_P1 5830 589 96.2 globlastp 1874switchgrass|gb167|DN150389 5831 589 95.7 globlastp 1875rice|11v1|AA752205 5832 589 93.3 globlastp 1876brachypodium|12v1|BRADI2G52777_P1 5833 589 88.1 globlastp 1877oat|11v1|CN820506_P1 5834 589 88.1 globlastp 1878pseudoroegneria|gb167|FF365257 5835 589 87.1 globlastp 1879wheat|12v3|BE404799 5836 589 86.7 globlastp 1880 barley|12v1|BF624474_P15837 589 86.2 globlastp 1881 leymus|gb166|EG392549_P1 5838 589 85.7globlastp 1882 oat|11v1|GO593702_T1 5839 589 84.76 glotblastn 1883rye|12v1|DRR001012.107131 5840 589 83.8 globlastp 1884lolium|10v1|ES699534_P1 5841 589 82.9 globlastp 1885sorghum|12v1|SB02G036080 5842 590 89.5 globlastp 1886switchgrass|12v1|DN143417_P1 5843 590 87.1 globlastp 1887switchgrass|12v1|FE607440_P1 5844 590 84.7 globlastp 1888switchgrass|gb167|DN143417 5845 590 84.2 globlastp 1889foxtail_millet|11v3|PHY7SI032776M_P1 5846 590 83.3 globlastp 1890sugarcane|10v1|CA067567 5847 590 81.88 glotblastn 1891sorghum|12v1|SB09G024370 5848 591 90.8 globlastp 1892foxtail_millet|11v3|PHY7SI022187M_P1 5849 591 86.8 globlastp 1893switchgrass|12v1|DN142345_P1 5850 591 84.4 globlastp 1894switchgrass|gb167|DN142345 5851 591 83.7 globlastp 1895switchgrass|12v1|FE645382_P1 5852 592 83 globlastp 1896sugarcane|10v1|CA103945 5853 592 92.7 globlastp 1897foxtail_millet|11v3|PHY7SI026860M_P1 5854 592 89.8 globlastp 1898sorghum|12v1|SB05G002140 5855 592 85.6 globlastp 1899 rice|11v1|AU0559105856 592 80.4 globlastp 1900 wheat|12v3|CA617395 5857 593 95.8 globlastp1901 maize|10v1|AI855306_P1 5858 593 91.7 globlastp 1902sorghum|12v1|SB012VICUFF33786T3 5859 593 90.6 globlastp 1903foxtail_millet|11v3|PHY7SI003476M_P1 5860 593 87.6 globlastp 1904switchgrass|12v1|SRR187769.1032073_P1 5861 593 81.2 globlastp 1905foxtail_millet|11v3|EC612302_P1 5862 594 90.5 globlastp 1906switchgrass|gb167|DN146578 5863 594 89.66 glotblastn 1907switchgrass|12v1|FL988994_P1 5864 594 88.3 globlastp 1908sorghum|12v1|SB06G022600 5865 594 87.8 globlastp 1909 rice|11v1|AA7519695866 594 81.03 glotblastn 1910 sorghum|12v1|SB02G038410 5867 595 92.1globlastp 1911 maize|10v1|AW120311_P1 5868 595 91.4 globlastp 1912sugarcane|10v1|CA164284 5869 595 84.79 glotblastn 1913sorghum|12v1|SB04G0270640 5870 596 90.7 globlastp 1914switchgrass|12v1|FL786140_P1 5871 596 89.1 globlastp 1915switchgrass|gb167|FL721485 5872 596 88.7 globlastp 1916switchgrass|12v1|FL721485_P1 5873 596 87.5 globlastp 1917foxtail_millet|11v3|PHY7SI017900M_P1 5874 596 87.5 globlastp 1918millet|10v1|CD726376_P1 5875 596 87.1 globlastp 1919cenchrus|gb166|EB657574_P1 5876 596 86.3 globlastp 1920cynodon|10v1|ES295019_P1 5877 596 83.1 globlastp 1921foxtail_millet|11v3|PHY7SI010534M_P1 5878 598 90.7 globlastp 1922brachypodium|12v1|BRADI5G17650T2_P1 5879 598 88.2 globlastp 1923barley|12v1|BM097165_P1 5880 598 87.2 globlastp 1924rye|12v1|DRR001012.110300 5881 598 87 globlastp 1925switchgrass|12v1|FE640105_P1 5882 598 86.4 globlastp 1926switchgrass|12v1|FE631813_P1 5883 598 85.2 globlastp 1927wheat|12v3|BE500130 5884 598 82.4 globlastp 1928 barley|12v1|BQ753928_P15885 598 82.1 globlastp 1929 wheat|12v3|CA607637 5886 598 81.7 globlastp1930 foxtail_millet|11v3|PHY7SI017738M_P1 5887 598 81.2 globlastp 1931switchgrass|12v1|FE613606_P1 5888 598 81 globlastp 1932brachypodium|12v1|BRADI3G50660_P1 5889 598 80.2 globlastp 1933switchgrass|gb167|FE613606 5890 598 80.2 globlastp 1934sorghum|12v1|SB04G032930 5891 598 80.1 globlastp 1935sorghum|12v1|SB05G022970 5892 599 86.9 globlastp 1936foxtail_millet|11v3|EC612732_P1 5893 599 82.7 globlastp 1937switchgrass|12v1|FL902137_P1 5894 599 81.1 globlastp 1938switchgrass|12v1|DN142479_T1 5895 599 81.01 glotblastn 1939sorghum|12v1|SB07G005620_P1 5896 600 86.6 globlastp 1940foxtail_millet|11v3|PHY7SI013963M_P1 5897 600 82 globlastp 1941switchgrass|12v1|FL825596_T1 5898 600 80.7 globlastp 1942sorghum|12v1|SB10G012990 5899 601 95.7 globlastp 1943switchgrass|12v1|FE607208_P1 5900 601 92.7 globlastp 1944rice|11v1|AU083373 5901 601 90.3 globlastp 1945 wheat|12v3|BE402176 5902601 89.8 globlastp 1946 brachypodium|12v1|BRADI1G42580_P1 5903 601 89globlastp 1947 rye|12v1|DRR001012.153298 5904 601 88.14 glotblastn 1948sugarcane|10v1|CA119301 5905 601 86.3 globlastp 1949switchgrass|12v1|SRR187765.274141_P1 5906 601 86 globlastp 1950sugarcane|10v1|BQ533976 5907 601 82.43 glotblastn 1951barley|12v1|BF262112_P1 5908 601 82.2 globlastp 1952leymus|gb166|EG380361_P1 5909 601 81.9 globlastp 1953rye|12v1|DRR001012.108416 5910 601 81.62 glotblastn 1954maize|10v1|AI941780_T1 5911 601 80.81 glotblastn 1955brachypodium|12v1|BRADI3G53200_T1 5912 601 80.54 glotblastn 1956wheat|12v3|BE604097 5913 901 80.54 glotblastn 1957sorghum|12v1|SB04G030050 5914 601 80.3 globlastp 1958switchgrass|gb167|DN147068 5915 601 80.11 glotblastn 1959switchgrass|12v1|DN147068_P1 5916 601 80 globlastp 1960foxtail_millet|11v3|PHY7SI0171324M_P1 5917 601 80 globlastp 1961rice|11v1|AU031830 5918 601 80 globlastp 1962 sugarcane|10v1|CA0782685919 602 96.2 globlastp 1963 sorghum|12v1|SB07G006400 5920 602 95.7globlastp 1964 foxtail_millet|11v3|PHY7SI014254M_T1 5921 602 93.65glotblastn 1965 rice|11v1|AU070756 5922 602 90.9 globlastp 1966switchgrass|12v1|FL973471_P1 5923 602 89 globlastp 1967millet|10v1|EVO454PM089385_P1 5924 602 86.6 globlastp 1968switchgrass|12v1|HO310867_P1 5925 602 82.3 globlastp 1969pseudoroegneria|gb167|FF345388 5926 602 81.2 globlastp 1970rye|12v1|BE704630 5927 602 81.2 globlastp 1971 rye|12v1|DRR001012.1746865928 602 81.2 globlastp 1972 wheat|12v3|CA598158 5929 602 81.2 globlastp1973 brachypodium|12v1|BRADI3G18320_P1 5930 602 80.4 globlastp 1974sorghum|12v1|SB03G038750 5931 603 95.2 globlastp 1975foxtail_millet|11v3|PHY7SI001054M_P1 5932 603 91.3 globlastp 1976rice|11v1|CA763022 5933 603 88 globlastp 1977brachypodium|12v1|BRADI2G53970_P1 5934 603 85.7 globlastp 1978wheat|12v3|CA499352 5935 603 84.3 globlastp 1979 wheat|12v3|BE5920485936 603 83.9 globlastp 1980 rye|12v1|BE494441 5937 603 83.7 globlastp1981 wheat|12v3|CA597218 5938 603 80.3 globlastp 1982sorghum|12v1|SB03G041150 5939 604 92.8 globlastp 1983switchgrass|12v1|FL765239_P1 5940 604 88 globlastp 1984foxtail_millet|11v3|PHY7SI004487M_P1 5941 604 88 globlastp 1985millet|10v1|EVO454PM018951_P1 5942 604 82.7 globlastp 1986foxtail_millet|11v3|EC612408_T1 5943 605 90.65 glotblastn 1987sorghum|12v1|SB10G023130 5944 605 85.9 globlastp 1988sugarcane|10v1|CA066507 5945 605 85.7 globlastp 1989 rice|11v1|BI3065145946 605 84.3 globlastp 1990 brachypodium|12v1|BRADI1G32020_T1 5947 60583.88 glotblastn 1991 oat|11v1|CN817174_T1 5948 605 83.88 glotblastn1992 rye|12v1|DRR001012.106695 5949 605 83.61 glotblastn 1993wheat|12v3|BE411964 5950 605 83.14 glotblastn 1994millet|10v1|EVO454PM001039_P1 5951 605 83.1 globlastp 1995switchgrass|12v1|FE618188_P1 5952 605 83.1 globlastp 1996switchgrass|gb167|FE618188 5953 605 83.1 globlastp 1997cenchrus|gb166|EB652675_P1 5954 605 82.9 globlastp 1998barley|12v1|BF624975_T1 5955 605 82.44 glotblastn 1999sorghum|12v1|SB07G026140 5956 606 96.6 globlastp 2000foxtail_millet|11v3|PHY7SI013455M_P1 5957 606 93.9 globlastp 2001maize|10v1|CD996489_P1 5958 606 93.5 globlastp 2002 rice|11v1|CA7484575959 606 87.9 globlastp 2003 brachypodium|12v1|BRADI1G41320_P1 5960 60686.7 globlastp 2004 barley|12v1|AK359805_P1 5961 606 86.1 globlastp 2005rye|12v1|BE494872 5962 606 85.9 globlastp 2006 wheat|12v3|CD880934 5963606 85.21 glotblastn 2007 sorghum|12v1|AW284970 5964 607 87.5 globlastp2008 sorghum|12v1|SB01G009530 5964 607 87.5 globlastp 2009maize|10v1|AW059954_P1 5965 607 85.7 globlastp 2010sugarcane|10v1|BQ530027 5966 607 83.57 glotblastn 2011sorghum|12v1|SB09G022630 5967 608 90.8 globlastp 2012switchgrass|12v1|FL993342_P1 5968 608 89 globlastp 2013cenchrus|gb166|EB669554_P1 5969 608 88.1 globlastp 2014foxtail_millet|11v3|PHY7SI023197M_P1 5970 608 88.1 globlastp 2015wheat|12v3|BE604061 5971 608 83.5 globlastp 2016 rice|11v1|BI813410 5972608 83.1 globlastp 2017 switchgrass|gb167|FL993342 5973 608 83.1globlastp 2018 rye|12v1|DRR001012.828307XX1 5974 608 83 globlastp 2019barley|12v1|BF626716_P1 5975 608 81.2 globlastp 2020brachypodium|12v1|BRADI2G22030_P1 5976 608 80.9 globlastp 2021sugarcane|10v1|CA091367 5977 609 91.2 globlastp 2022sorghum|12v1|SB06G023260 5978 609 90.5 globlastp 2023foxtail_millet|11v3|PHY7SI011332M_P1 5979 609 87.3 globlastp 2024millet|10v1|EVO454PM326349_P1 5980 609 85.5 globlastp 2025switchgrass|12v1|FE635833_P1 5981 609 82.3 globlastp 2026switchgrass|12v1|FL965224_P1 5982 609 82.3 globlastp 2027switchgrass|gb167|FE635833 5982 609 82.3 globlastp 2028sorghum|12v1|SB10G005040 5983 610 96.1 globlastp 2029sugarcane|10v1|CA216130 5984 610 94.8 globlastp 2030foxtail_millet|11v3|PHY7SI007419M_P1 5985 610 91.6 globlastp 2031wheat|12v3|CA484155 5986 610 89.6 globlastp 2032switchgrass|gb167|FL980407 5987 610 88.4 globlastp 2033millet|10v1|EVO454PM167260_P1 5988 610 88.3 globlastp 2034switchgrass|12v1|FL980407_P1 5989 610 87.7 globlastp 2035switchgrass|12v1|GD015248_P1 5990 610 87 globlastp 2036switchgrass|gb167|GD015248 5991 610 87 globlastp 2037lovegrass|gb167|DN480312_P1 5992 610 81.4 globlastp 2038sorghum|12v1|SB04G006020 5993 611 92.9 globlastp 2039maize|10v1|AW054575_P1 5994 611 92.8 globlastp 2040switchgrass|12v1|FL873296_P1 5995 611 92.1 globlastp 2041foxtail_millet|11v3|PHY7SI018649M_P1 5996 611 92.1 globlastp 2042sugarcane|10v1|CA289508 5997 611 92.1 globlastp 2043maize|10v1|ZMCRP2V222510_T1 5998 611 90.2 glotblastn 2044sorghum|12v1|SB12VOCRP053042 5999 611 86.49 glotblastn 2045millet|10v1|EVO454PM554128_P1 6000 611 85.6 globlastp 2046rice|11v1|BE230517 6001 611 80.8 globlastp 2047switchgrass|12v1|FL691774_P1 6002 611 90.7 globlastp 2048foxtail_millet|11v3|PHY7SI005698M_P1 6003 611 90 globlastp 2049rice|11v1|BM038357_P1 6004 613 88.6 globlastp 2050brachypodium|12v1|BRADI1G38025_P1 6005 613 88.2 globlastp 2051switchgrass|12v1|FL810628_P1 6006 613 83.7 globlastp 2052wheat|12v3|SRR073321X137461D1_T1 6007 613 81.64 glotblastn 2053sorghum|12v1|SB02G009130 6008 614 85.3 globlastp 2054foxtail_millet|11v3|PHY7SI028698M_P1 6009 614 84.3 globlastp 2055foxtail_millet|11v3|PHY7SI028688M_P1 6010 614 84.2 globlastp 2056sorghum|12v1|SB02G009140 6011 614 83.12 glotblastn 2057switchgrass|12v1|FE627523_P1 6012 614 83 globlastp 2058brachypodium|12v1|BRADI1G57240_T1 6013 614 80.2 glotblastn 2059sorghum|12v1|SB06G015410 6014 615 91.5 globlastp 2060switchgrass|gb167|FE628616 6015 615 91.1 globlastp 2061foxtail_millet|11v3|PHY7SI010641M_P1 6016 615 90.4 globlastp 2062switchgrass|12v1|FE628616_P1 6017 615 89.9 globlastp 2063sorghum|12v1|SB06G015400 6018 615 88.6 globlastp 2064brachypodium|12v1|BRADI5G09180_P1 6019 615 86.4 globlastp 2065barley|12v1|BF261816_P1 6020 615 85.4 globlastp 2066rye|12v1|DRR001012.136309 6021 615 85.4 globlastp 2067wheat|12v3|CA694027 6022 615 85.13 glotblastn 2068pseudoroegneria|gb167|FF346018 6023 615 85.1 globlastp 2069wheat|12v3|BE638108 6024 615 84.8 globlastp 2070 wheat|12v3|BI4796956025 615 83.2 globlastp 2071 wheat|12v3|CA618982 6026 616 98.53glotblastn 2072 sorghum|12v1|SB06G025770 6027 618 93.3 globlastp 2073foxtail_millet|11v3|PHY7SI010516M_P1 6028 618 91.6 globlastp 2074switchgrass|12v1|FL941640_P1 6029 618 91 globlastp 2075switchgrass|12v1|FE618003_P1 6030 618 89.8 globlastp 2076switchgrass|gb167|FE618003 6030 618 89.8 globlastp 2077millet|10v1|CD725035_P1 6031 618 84.4 globlastp 2078brachypodium|12v1|BRADI5G18750_P1 6032 618 81.5 globlastp 2079switchgrass|12v1|SRR187765.696295_T1 6033 619 86.25 glotblastn 2080sorghum|12v1|SB03G036325 6034 619 84.15 glotblastn 2081switchgrass|12v1|FL786883_T1 6035 619 83.75 glotblastn 2082lovegrass|gb167|DN480814_P1 6036 619 80.7 globlastp 2083foxtail_millet|11v3|PHY7SI003577M_P1 6037 619 80.5 globlastp 2084foxtail_millet|11v3|PHY7SI010398M_P1 6038 621 83.6 globlastp 2085switchgrass|gb167|FE606354 6039 621 81.9 globlastp 2086millet|10v1|CD725601_P1 6040 621 81.8 globlastp 2087switchgrass|12v1|DN141325_P1 6041 622 80.5 globlastp 2088sorghum|12v1|SB07G025120 6042 622 80.35 glotblastn 2089sorghum|12v1|SB01G016420 6043 623 89.7 globlastp 2090foxtail_millet|11v3|PHY7SI039879M_P1 6044 623 85.8 globlastp 2091sorghum|12v1|SB02G029320 6045 624 86.7 globlastp 2092maize|10v1|CF043867_P1 6046 624 83.1 globlastp 2093millet|10v1|EVO454PM458631_P1 6047 624 82 globlastp 2094foxtail_millet|11v3|PHY7SI031193M_P1 6048 624 81.2 globlastp 2095sorghum|12v1|SB03G043280 6049 625 94.2 globlastp 2096foxtail_millet|11v3|PHY7SI005148M_T1 6050 625 87.67 glotblastn 2097foxtail_millet|11v3|PHY7SI002757M_P1 6051 627 86.1 globlastp 2098sorghum|12v1|SB03G009060 6052 627 84.1 globlastp 2099switchgrass|12v1|FL726886_P1 6053 627 82.1 globlastp 2100sorghum|12v1|SB01G046350 6054 629 86.9 globlastp 2101sugarcane|10v1|CA074330 630 630 100 globlastp 2102sorghum|12v1|SB07G022630 6055 630 99.6 globlastp 2103switchgrass|12v1|DN140969_P1 6056 630 98.4 globlastp 2104switchgrass|gb167|DN140969 6056 630 98.4 globlastp 2105switchgrass|12v1|FE645210_P1 6056 630 98.4 globlastp 2106switchgrass|gb167|DN645210 6056 630 98.4 globlastp 2107cenchrus|gb166|EB657452_P1 6057 630 98 globlastp 2108foxtail_millet|11v3|EC613322_P1 6058 630 98 globlastp 2109barley|12v1|BE411312_P1 6059 630 95.9 globlastp 2110brachypodium|12v1|BRADI1G1990_P1 6060 630 95.9 globlastp 2111oat|11v1|GO581906_P1 6061 630 95.9 globlastp 2112pseudoroegneria|gb167|FF343605 6059 630 95.9 globlastp 2113wheat|12v3|BE419677 6059 630 95.9 globlastp 2114 lolium|10v1|AU245785|T16062 630 95.51 glotblastn 2115 leymus|gb166|EG396954_P1 6063 630 95.5globlastp 2116 rice|11v1|BI802985 6064 630 95.5 globlastp 2117rye|12v1|BE704628 6065 630 95.5 globlastp 2118 rye|12v1|DRR001012.1255796065 630 95.5 globlastp 2119 oat|11v1|GO583055_P1 6066 630 95.1globlastp 2120 peanut|10v1|EE127200_P1 6067 630 93.9 globlastp 2121peanut|10v1|ES724140_P1 6068 630 93.9 globlastp 2122pigeonpea|11v1|SRR054580X109465_P1 6069 630 93.9 globlastp 2123cowpea|12v1|FF391074_P1 6070 630 93.5 globlastp 2124oil_palm|11v1|EY411468_P1 6071 630 93.5 globlastp 2125pigeonpea|11v1|SRR054580X109236_P1 6072 630 93.5 globlastp 2126banana|12v1|BBS1822T3_P1 6073 630 93.1 globlastp 2127 bean|12v1|CA8981126074 630 93.1 globlastp 2128 cowpea|12v1|FF395868_P1 6075 630 93.1globlastp 2129 flaveria|11v1|SRR149229.14060_P1 6076 630 93.1 globlastp2130 flaveria|11v1|SRR149229.271685_P1 6076 630 93.1 globlastp 2131flaveria|11v1|SRR149232.133345_P1 6077 630 93.1 globlastp 2132flaveria|11v1|SRR149232.23150_P1 6076 630 93.1 globlastp 2133soybean|11v1|GLYMA09G28640 6078 630 93.1 globlastp 2134soybean|11v1|GLYMA09G28640_P1 6078 630 93.1 globlastp 2135soybean|11v1|GLYMA16G33360 6079 630 93.1 globlastp 2136soybean|12v1|GLYMA16G33370T4_P1 6079 630 93.1 globlastp 2137soybean|11v1|GLYMA20G35750 6080 630 93.1 globlastp 2138soybean|12v1|GLYMA20G35750_P1 6080 630 93.1 globlastp 2139bean|12v2|CA899890_P1 6081 630 92.7 globlastp 2140cirsium|11v1|SRR346952.1018515_P1 6082 630 92.7 globlastp 2141cirsium|11v1|SRR346952.111639_P1 6082 630 92.7 globlastp 2142coffea|10v1|DV666851_P1 6083 630 92.7 globlastp 2143dandelion|10v1|DR399058_P1 6084 630 92.7 globlastp 2144lettuce|12v1|DW063622_P1 6085 630 92.7 globlastp 2145platanus|11v1|SRR096786X101590_P1 6086 630 92.7 globlastp 2146centaurea|11v1|EH778178_P1 6082 630 92.7 globlastp 2147centaurea|11v1|EH718595_P1 6082 630 92.7 globlastp 2148centaurea|11v1|EH717068_T1 6087 630 92.65 glotblastn 2149ambrosia|11v1|SRR346946.101372_T1 6088 630 92.24 glotblastn 2150prunus_mume|13v1|BU039610_P1 6089 630 92.2 globlastp 2151ambrosia|11v1|SRR346935.325236_P1 6090 630 92.2 globlastp 2152ambrosia|11v1|SRR346943.104742_P1 6090 630 92.2 globlastp 2153arnica|11v1|SRR099034X132474_P1 6090 630 92.2 globlastp 2154chelidonium|11v1|SRR084752X107741_P1 6091 630 92.2 globlastp 2155flaveria|11v1|SRR14229.163723_P1 6090 630 92.2 globlastp 2156hornbeam|12v1|SRR364455.105073_P1 6092 630 92.2 globlastp 2157ipomoea_nil|10v1|CJ739977_P1 9093 630 92.2 globlastp 2158prunus|10v1|BU039610 9089 630 92.2 globlastp 2159sunflower|12v1|CD851643 6090 630 92.2 globlastp 2160sunflower|12v1|CD851651 6090 630 92.2 globlastp 2161sunflower|12v1|EE633004 6090 630 92.2 globlastp 2162olea|13v1|SRR014464X11134D1_P1 6094 630 92.2 globlastp 2163centaurea|gb166|EH766091 6095 630 91.84 glotblastn 2164gerbera|09v1|AJ762871_T1 6096 630 91.84 glotblastn 2165platanus|11v1|SRR096786X103251_T1 6097 630 91.84 glotblastn 2166sunflower|12v1|ERR029545X40874 6098 630 91.84 glotblastn 2167ambrosia|11v1|SRR346943.116300_P1 6099 630 91.8 globlastp 2168apple|11v1|CN876664_P1 6100 630 91.8 globlastp 2169cassava|09v1|DV441101_P1 6101 630 91.8 globlastp 2170castorbean|12v1|GE633432_P1 6102 630 91.8 globlastp 2171centaurea|gb166|EH717068 6103 630 91.8 globlastp 2172eucalyptus|11v2|CD668611_P1 6104 630 91.8 globlastp 2173oil_palm|11v1|EL693601_P1 6105 630 91.8 globlastp 2174phyla|11v2|SRR099035X101156_P1 6016 630 91.8 globlastp 2175vinca|11v1|SRR098690X104175 6107 630 91.8 globlastp 2176blueberry|12v1|SRR353282X1807D1_P1 6108 630 91.43 glotblastn 2177fraxinus|11v1|SRR058827.113024_T1 6109 630 91.43 glotblastn 2178olea|13v1|SRR592583X161174D1_P1 6110 630 91.4 globlastp 2179amorphophallus|11v2|SRR089351X102088_P1 6111 630 91.4 globlastp 2180amsonia|11v1|SRR098688X107510_P1 6112 630 91.4 globlastp 2181aristolochia|10v1|FD750472_P1 6113 630 91.4 globlastp 2182chickpea|11v1|SRR133517.148214 6114 630 91.4 globlastp 2183chickpea|13v2|SRR133517.148214_P1 6114 630 91.4 globlastp 2184cotton|11v1|CO092137_P1 6115 630 91.4 globlastp 2185ginger|gb164|DY345402_P1 6116 630 91.4 globlastp 2186gossypium_raimondii|12v1|BG442956_P1 6115 630 91.4 globlastp 2187rose|12v1|BQ104637 6117 630 91.4 globlastp 2188tabernaemontana|11v1|SRR098689X102299 6118 630 91.4 globlastp 2189tobacco|gb162|CV019233 6119 630 91.4 globlastp 2190banana|12v1|FL647015_T1 6120 630 91.02 glotblastn 2191eschscholzia|11v1|SRR014116.122571_T1 6121 630 91.02 glotblastn 2192bean|12v2|CA898112_P1 6122 630 91 globlastp 2193artemisia|10v1|EY074664_P1 6123 630 91 globlastp 2194basilicum|10v1|DY325692_P1 6124 630 91 globlastp 2195blueberry|12v1|SRR353282X14043D1_P1 6125 630 91 globlastp 2196cacao|10v1|CU481646_P1 6126 630 91 globlastp 2197cotton|11v1|CO089165_P1 6127 630 91 globlastp 2198humulus|11v1|EX518234_P1 6128 630 91 globlastp 2199ipomoea_nuk|10v1|BJ563148_P1 6129 630 91 globlastp 2200monkeyflower|10v1|CV520116 6130 630 91 globlastp 2201monkeyflower|12v1|CV520116_P1 6130 630 91 globlastp 2202nasturtium|11v1|SRR032558.102424_P1 6131 630 91 globlastp 2203orange|11v1|CB305130_P1 6132 630 91 globlastp 2204pepper|12v1|GD094722_P1 6133 630 91 globlastp 2205strawberry|11v1|SRR034866S0006579 6134 630 91 globlastp 2206tomato|11v1|BG133635 6135 630 91 globlastp 2207triphysaria|10v1|EY128411 6136 630 91 globlastp 2208vinca|11v1|SRR098690X119964 6137 630 91 globlastp 2209nicotiana_benthamiana|12v1|BP744332_T1 6138 630 90.61 glotblastn 2210poplar|13v1|AI165542_P1 6139 630 90.6 globlastp 2211catharanthus|11v1|SRR098691X111547_P1 6140 630 90.6 globlastp 2212clementine|11v1|CB305130_P1 6141 630 90.6 globlastp 2213gossypium_raimondii|12v1|BG445100_P1 6142 630 90.6 globlastp 2214grape|11v1|GSVIVT01022313001_P1 6143 630 90.6 globlastp 2215humulus|11v1|EX516492_P1 6144 630 90.6 globlastp 2216jatropha|09V1|GT228596_P1 6145 630 90.6 globlastp 2217kiwi|gb166|FG412659_P1 6146 630 90.6 globlastp 2218orange|11v1|CF830428_P1 6147 630 90.6 globlastp 2219poplar|10v1|AI165542 6139 630 90.6 globlastp 2220potato|10v1|BG095663_P1 6148 630 90.6 globlastp 2221sesame|12v1|BU670488 6149 630 90.6 globlastp 2222solanum_phureja|-0v1|SPHBG133635 6148 630 90.6 globlastp 2223triphysaria|10v1|EX989885 6150 630 90.6 globlastp 2224vinca|11v1|SRR098690X101652 6151 630 90.6 globlastp 2225aquilegia|10v2|DR918066_P1 6152 630 90.2 globlastp 2226arabidopsis_lyrata|09v1|JGIAL018820_P1 6153 630 90.2 globlastp 2227canola|11v1|DY030272_P1 6154 630 90.2 globlastp 2228clementine|11v1|CF830428_P1 6155 630 90.2 globlastp 2229euphorbia|11v1|SRR098678X133629_P1 6156 630 90.2 globlastp 2230fagopyrum|11v1|SRR063703X101104_P1 6157 630 90.2 globlastp 2231radish|gb164|EV528078 6158 630 90.2 globlastp 2232 radish|gb164|EV5462586159 630 90.2 globlastp 2233 radish|gb164|EW731449 6158 630 90.2globlastp 2234 silene|11v1|SRR096785X153172 6160 630 90.2 globlastp 2235tripterygium|11v1|SRR098677X104866 6161 630 90.2 globlastp 2236tripterygium|11v1|SRR098677X115523 6162 630 90.2 globlastp 2237valeriana|11v1|SRR099039X110396 6163 630 90.2 globlastp 2238arabidopsis|10v1|AT3G55620_P1 6164 630 89.8 globlastp 2239b_rapa|11v1|CD830217_P1 6165 630 89.8 globlastp 2240canola|11v1|CN735659_P1 6166 630 89.8 globlastp 2241canola|11v1|EE405557_P1 6165 630 89.8 globlastp 2242lovegrass|gb167|EH184810_P1 6167 630 89.8 globlastp 2243strawberry|11v1|DV440665 6168 630 89.8 globlastp 2244canola|11v1|CN730372_P1 6169 630 89.4 globlastp 2245podocarpus|10v1|SRR065014S0011263_P1 6170 630 89.4 globlastp 2246b_rapa|11v1|CX187538_P1 6171 630 89 globlastp 2247catharanthus|11v1|SRR0989691X113394_P1 6172 630 89 globlastp 2248chickpea|11v1|FE672990 6173 630 89 globlastp 2249chickpea|13v2|FE672990_P1 6173 630 89 globlastp 2250euonymus|11v1|SRR070038X172289_P1 6174 630 89 globlastp 2251euonymus|11v1|SRR070038X414930_P1 6174 630 89 globlastp 2252monkeyflower|10v1|GR067455 6175 630 89 globlastp 2253monkeyflower|12v1|GR067455_P1 6175 630 89 globlastp 2254phyla|11v2|SRR099037X113124_P1 6176 630 89 globlastp 2255silene|11v1|GH292754 6177 630 89 globlastp 2256silene|11v1|SRR096785X102821 6178 630 89 globlastp 2257thellungiella_parvulum|11v1|DN773718 6179 630 88.98 glotblastn 2258thellungiella_parvulum|11v1|EPCRP019003 6180 630 88.6 globlastp 2259cacao|10v1|CU472943_P1 6181 630 88.6 globlastp 2260poppy|11v1|SRR030259.114710_P1 6182 630 88.6 globlastp 2261primula|11v1|SRR098679X121424_P1 6183 630 88.6 globlastp 2262watermelon|11v1|AB182929 6184 630 88.6 globlastp 2263flax|11v1|JG026727_T1 6185 630 82.57 glotblastn 2264medicago|12v1|SM_003622325_T1 6186 630 82.57 glotblastn 2265sarracenial|11v1|SRR192669.129090 6187 630 82.57 glotblastn 2266spurge|gb161|DV125160 6188 630 82.57 glotblastn 2267utricularial|11v1|SRR094438.122965 6189 630 88.21 glotblastn 2268zostera|12v1|SRR057351X106670D1_P1 6190 630 88.2 globlastp 2269kiwi|gb166|FG484183_P1 6191 630 88.2 globlastp 2270 poplar|10v1|AI1646466192 630 88.2 globlastp 2271 poplar|13v1|AI164646_P1 6192 630 88.2globlastp 2272 poppy|11v1|SRR030259.12644_P1 6193 630 88.2 globlastp2273 sciadopitys|10v1|SRR065035S0036449 6194 630 88.2 globlastp 2274taxus|10v1|SRR032523S0028155 6195 630 88.2 globlastp 2275zostera|10v1|SRR057351S0004096 6190 630 88.2 globlastp 2276thellungiella_halophilum|11v1|DN773718 6196 630 88.16 glotblastn 2277ambrosia|11v1|SRR346943.107730_T1 6197 630 87.8 glotblastn 2278clover|gb162|AB236812_P1 6198 630 87.8 globlastp 2279curcurbita|11v1|FG227176_P1 6199 630 87.8 globlastp 2280distylium|11v1|SRR065077X101454_P1 6200 630 87.8 globlastp 2281flaveria|11v1|SRR149229.190286_P1 6201 630 87.8 globlastp 2282medicago|12v1|BE240689_P1 6202 630 87.8 globlastp 2283melon|10v1|EB715694_P1 6203 630 87.8 globlastp 2284phalaenopsis|11v1|SRR125771.1006537_P1 6204 630 87.8 globlastp 2285phalaenopsis|11v1|SRR125771.101173 6204 630 87.8 globlastp 2286pine|10v2|BM903098_P1 6205 630 87.8 globlastp 2287cannabis|12v1|SOLX00042286_P1 6206 630 87.6 globlastp 2288beet|12v1|AJ009737_P1 6207 630 87.4 globlastp 2289beech|11v1|SRR006294.12431_P1 6208 630 87.3 globlastp 2290maritime_pine|10v1|CR392997_P1 6289 630 87.3 globlastp 2291oak|10v1|SRR039734S0110825_P1 6210 630 87.3 globlastp 2292pine|10v2|AA557111_P1 6211 630 87.3 globlastp 2293 pine|10v2|AW226483_P16211 630 87.3 globlastp 2294 spruce|11v1|EX365100 6212 630 87.3globlastp 2295 spruce|11v1|SRR064180X498651 6212 630 87.3 globlastp 2296radish|gb164|EV528120 6213 630 87 globlastp 2297maritime_pine|10v1|SRR073317S0018081_T1 6214 630 86.94 glotblastn 2298abies|11v2|SRR098676X100878_P1 6215 630 86.9 globlastp 2299cedrus|11v1|SRR065007X116945_P1 6216 630 86.9 globlastp 2300cryptomeria|gb166|BW994649_P1 6217 630 86.9 globlastp 2301cucumber|09v1|EB715694_P1 6218 630 86.9 globlastp 2302eschscholzia|11v1|SRR014116.100907 6219 630 86.9 globlastp 2303euonymus|11v1|SRR070038X112495_P1 6220 630 86.9 globlastp 2304melon|10v1|MEL01698615123105_P1 6221 630 86.9 globlastp 2305momordical|10v1|SRR071315S0001316_P1 6222 630 86.9 globlastp 2306petunia|gb171|CV295494_P1 6223 630 86.9 globlastp 2307spruce|11v1|ES253826 6224 630 86.9 globlastp 2308 spruce|11v1|ES8677096225 630 86.9 globlastp 2309 spruce|11v1|ES873855 6226 630 86.9globlastp 2310 spruce|11v1|EX36195 6224 630 86.9 globlastp 2311zamia|gb166|DY030845 6227 630 86.9 globlastp 2312peach|gb157.2|BU039610_P1 6228 630 86.6 globlastp 2313oak|10v1|DB997678_T1 6229 630 86.53 glotblastn 2314maritime_pine|10v1|SRR073317S0142793_T1 — 630 86.53 glotblastn 2315cephalotaxus|11v1|SRR064395X100214_P1 6230 630 86.5 globlastp 2316chestnut|gb170|SRR006295S0008134_P1 6231 630 86.5 globlastp 2317cycas|gb166|CB088723_P1 6232 630 86.5 globlastp 2318pseudotsuga|10v1|SRR065119S0002809 6233 630 86.5 globlastp 2319spruce|11v1|CO237150 6234 630 86.5 globlastp 2320fagopyrum|11v1|SRR063689X102968_P1 6235 630 86.2 globlastp 2321flaveria|11v1|sRR149229.107405_T1 6236 630 86.12 glotblastn 2322pine|10v2|BX254326_T1 6237 630 86.12 glotblastn 2323cucumber|09v1|V633817_P1 6238 630 86.1 globlastp 2324pine|10v2|GW754514_T1 6239 630 85.71 glotblastn 2325centaurea|gb166|EH720361 6240 630 85.7 globlastp 2326cephalotaxus|11v1|SRR064395X125095_P1 6241 630 85.7 globlastp 2327cirsium|11v1|SRR346952.1010767_P1 6242 630 85.7 globlastp 2328cynara|gb167|GE579606_P1 6243 630 85.7 globlastp 2329maritime_pine|10v1|BX254326_P1 6244 630 85.7 globlastp 2330pigeonpea|11v1|CCIIPG11047741_P1 6245 630 85.7 globlastp 2331plantago|11v2|SRR066373X110993_P1 6246 630 85.7 globlastp 2332sequoia|10v1|SRR346943.201002_T1 6247 630 85.7 globlastp 2333watermelon|11v1|AM713836 6248 630 85.6 globlastp 2334ambrosia|11v1|SRR346943.201002_T1 6249 630 85.31 glotblastn 2335sunflower|12v1|AK539803 6250 630 85.3 globlastp 2336ambrosia|11v1|SRR346935.133099_P1 6251 630 84.9 globlastp 2337ambrosia|11v1|SRR346943.101825_P1 6252 630 84.9 globlastp 2338cichorium|gb171|EJ91582_P1 6253 630 84.9 globlastp 2339gnetum|1-v1|EX948582_P1 6254 630 84.9 globlastp 2340lettuce|12v1|DQ060380_P1 6255 630 84.9 globlastp 2341orobanche|10v1|SRR023189S0001320_P1 6256 630 84.9 globlastp 2342pteridium|11v1|SRR043594X105316 6257 630 84.9 globlastp 2343ambrosia|11v1|SRR346935.107518_P1 6258 630 84.5 globlastp 2344cotton|11v1|BG445200_P1 6259 630 84.5 globlastp 2345pigeonpea|11v1|SRR054580X101723_P1 6260 630 84.5 globlastp 2346arnia|11v1|SRR099034X19021_T1 6261 630 84.49 glotblastn 2347ambrosia|11v1|SRR346943.43314_P1 6262 630 84.1 globlastp 2348cirsium|11v1|SRR346952.106650_P1 6263 630 84.1 globlastp 2349dandelion|10v1|DY819410_P1 6264 630 83.7 globlastp 2350flaveria|11v1|sRR149229.135585_P1 6265 630 83.5 globlastp 2351ceratodon|10v1|SRR04890S0022247_P1 6266 630 83.3 globlastp 2352olea|11v1|SRR014464.11134 6267 630 83.3 globlastp 2353physcomitrella|10v1|BJ174885_P1 6268 630 83.3 globlastp 2354cichorium|gb171|EH690915_T1 6269 630 83.27 globlastp 2355physcomitrella|10v1|BJ174601_P1 6270 630 82.9 globlastp 2356radish|gb164|EV537845 6271 630 82.9 globlastp 2357antirrhinum|gb166|AJ94343_P1 6272 630 82.6 globlastp 2358millet|10v1|EVO454PM008575_P1 6273 630 82.4 globlastp 2359spikemoss|gb165|FE466723 6274 630 82 globlastp 2360poplar|10v1|POPTR0008S03180 6275 630 81.71 glotblastn 2361parthenium|10v1|GW778698_T1 6276 630 81.63 glotblastn 2362quizotia|10v1|GE573642_P1 6277 630 81.6 globlastp 2363fraxinus|11v1|SRR058827.110343_P1 6278 630 81.2 globlastp 2364spikemoss|gb165|FE467241 6279 630 81.2 globlastp 2365brachypodium|12v1|BRADI2G10857_P1 6280 630 80.9 globlastp 2366cleome_gynandra|10v1|SRR015532S0066348_P1 6281 630 80.8 globlastp 2367petunia|gb171|CV29721_P1 6282 630 80.4 globlastp 2368safflower|gb162|EL408052 6283 630 80.4 globlastp 2369centaurea|11v1|EH720361_P1 6284 630 80 globlastp 2370ambrosia|11v1|SRR346935.80808_P1 6285 630 80 globlastp 2371onion|12v1|SRR073446X111037D1_P1 6286 630 80 globlastp 2372sorghum|12v1|SB04G002670 6287 631 88.5 globlastp 2373maize|10v1|AW231612_P1 6288 631 85.4 globlastp 2374foxtail_millet|11v3|PHY7SI016813M_P1 6289 631 81.8 globlastp 2375sorghum|12v1|SB06G020170 6290 632 91.9 globlastp 2376maize|10v1|BE224944_P1 6291 632 91.7 globlastp 2377foxtail_millet|11v3|PHY7SI009805M_P1 6292 632 86.5 globlastp 2378foxtail_millet|11v3|SIPRD090813_T1 6293 632 84.86 glotblastn 2379switchgrass|12v1|FE612283_P1 9294 633 90.1 globlastp 2380brachypodium|12v1|BRADI1G08400_P1 6295 633 87.9 globlastp 2381rye|12v1|DRR001012.121069 6296 633 87.6 globlastp 2382wheat|12v3|CJ952926 6297 633 87 globlastp 2383 barley|12v1|AF406643_P16298 633 85.8 globlastp 2384 foxtail_millet|11v3|PHY7SI034030M_P1 6299633 83.5 globlastp 2385 switchgrass|12v1|FL716316_P1 6300 640 81.2globlastp 2386 switchgrass|gb167|FL716316 6301 640 81.15 glotblastn 2387rice|11v1|BI795969 6302 610 81.8 globlastp 2388brachypodium|12v1|BRADI3G47800_P1 6303 643 93.2 globlastp 2389wheat|12v3|BQ237777 6304 643 92.1 globlastp 2390foxtail_millet|11v3|EC613250_P1 6305 643 91.5 globlastp 2391sorghum|12v1|SB04G024980 6306 643 90.8 globlastp 2392maize|10v1|AI612429_P1 6307 643 90.5 globlastp 2393switchgrass|12v1|FE647683_P1 6308 643 90.3 globlastp 2394maize|10v1|AI737219_P1 6309 643 89.4 globlastp 2395oil_palm|11v1|SRR190698.108780_P1 6310 643 88.3 globlastp 2396oil_palm|11v1|EL692446_P1 6311 643 88.1 globlastp 2397amborella|12v3|SRR038635.94614_P1 6312 643 87.1 globlastp 2398banana|12v1|MAGEN2012018109_P1 6313 643 86.2 globlastp 2399amorphorphallus|11v2|SRR089351X111818_T1 6314 643 85.75 glotblastn 2400solanum_phureja|09v1|SPHAW737348 6315 643 85.5 globlastp 2401eucalyptus|11v2|CD668409_P1 6316 643 85.1 globlastp 2402tomato|11v1|AW737348 6317 643 85.1 globlastp 2403 zostera|10v1|AM7684546318 643 85.05 glotblastn 2404 cotton|11v1|AW587510_P1 6319 643 85globlastp 2405 gossypium_raimondii|12v1|AW587510_P1 6319 643 85globlastp 2406 phalaeopsis|11v1|SRR125771.1048758_P1 6320 643 85globlastp 2407 phyla|11v2|SRR099035X121463_T1 6321 643 84.88 glotblastn2408 castorbean|11v1|EG656596 6322 643 84.8 globlastp 2409castorbean|11v1|EG656596_P1 6322 643 84.8 globlastp 2410cucumber|09v1|DV737753_P1 6323 643 84.8 globlastp 2411watermelon|11v1|AM732207 6324 643 84.8 globlastp 2412nicotiana_benthamiana|12v1|FG135243_P1 6325 643 84.7 globlastp 2413tabernaemontana|11v1|SRR098689X112646 6326 643 84.65 glotblastn 2414cassava|09v1|FF534723_P1 6327 643 84.6 globlastp 2415melon|10v1|AM732207_P1 6328 643 84.6 globlastp 2416ambrosia|11v1|SRR346935.178950_P1 6329 643 84.5 globlastp 2417nicotiana_benthamiana|12v1|BP751817_P1 6330 643 84.4 globlastp 2418pigeonpea|11v1|SRR054580X1028_P1 6331 643 84.4 globlastp 2419valeriana|11v1|SRR099039X113384 6332 643 84.2 globlastp 2420banana|12v1|ES431755_P1 6333 643 84.1 globlastp 2421bean|12v1|SRR001335.264127 6334 643 84.1 globlastp 2422beet|12v1|BQ589904_P1 6335 643 84.1 globlastp 2423 oak|10v1|FP045293_P16336 643 84.1 globlastp 2424 poplar|10v1|AI163495 6337 643 84.1globlastp 2425 poplar|10v1|AI163495_P1 6337 643 84.1 globlastp 2426poplar|10v1|DT476741 6338 643 84.1 globlastp 2427poplar|10v1|DT476741_P1 6338 643 84.1 globlastp 2428ambrosia|11v1|SRR346935.100892_P1 6339 643 84 globlastp 2429banana|12v1|BBS21T3_P1 6340 643 84 globlastp 2430monkeyflower|10v1|GR060921 6341 643 84 globlastp 2431monkeyflower|10v1|GR113891_P1 6341 643 84 globlastp 2432ambrosia|11v1|SRR346935.118239_T1 6342 643 83.99 glotblastn 2433bean|12v1|SRR001335.264127_P1 6343 643 83.9 globlastp 2434soybean|12v1|GLYMA08G13770_P1 6344 643 83.9 globlastp 2435euphorbia|11v1|DV133781_P1 6345 643 83.9 globlastp 2436amsonia|11v1|SRR098688X106392_P1 6346 643 83.7 globlastp 2437beech|11v1|sRR006294.24743_T1 6347 643 83.68 glotblastn 2438peanut|10v1|GO268027_T1 6348 643 83.68 glotblastn 2439orange|11v1|CB304651_P1 6349 643 83.6 globlastp 2440spurge|gb161|DV133781 6350 643 83.6 globlastp 2441zostera|12v1|SRR057351X20488D1_T1 6318 643 83.41 glotblastn 2442apple|11v1|CN996714_P1 6351 643 83.4 globlastp 2443poppy|11v1|SRR030259.109492_P1 6352 643 83.4 globlastp 2444soybean|12v1|GLYMA05G30630 6353 643 83.4 globlastp 2445soybean|12v1|GLYMA05G30630_P1 6353 643 83.4 globlastp 2446tobacco|gb62|GFXAM711117X1 6354 643 83.4 globlastp 2447trigonella|11v1|SRR066194X103705 6355 643 83.4 globlastp 2448bean|12v2|FG229148_P1 6356 643 83.2 globlastp 2449strawberry|11v1|DY667941 6357 643 83.2 globlastp 2450flaveria|11v1|SRR149229.109258_P1 6358 643 83.1 globlastp 2451monkeyflower|10v1|GR021687 6359 643 83 globlastp 2452monkeyflower|10v1|GR171697_P1 6359 643 83 globlastp 2453prunus_mume|13v1|BU08518_P1 6360 643 82.9 globlastp 2454prunus|10v1|BU048518 6360 643 82.9 globlastp 2455chickpea|13v2|GR394918_P1 6361 643 82.8 globlastp 2456solanum_phureja|09v1|SPHAW217173 6362 643 82.8 globlastp 2457soybean|12v1|GLYMA08G13810 6363 643 82.8 globlastp 2458soybean|12v1|GLYMA08G13810_P1 6363 643 82.8 globlastp 2459tomato|11v1|AW217173 6364 643 82.8 globlastp 2460cirsium|11v1|SRR246952.1024359_P1 6365 643 82.7 globlastp 2461cotton|11v1|AW187180_P1 6366 643 82.7 globlastp 2462gossypium_raimondii|12v1|DT558220_P1 6367 643 82.7 globlastp 2463centaurea|11v1|EH725998_P1 6368 643 82.7 globlastp 2464apple|11v1|CN496063_P1 6369 643 82.5 globlastp 2465cassava|09v1|DB920810_P1 6370 643 82.5 globlastp 2466cotton|11v1|CO72400XX1_P1 6371 643 82.5 globlastp 2467lettuce|12v1|DW059332_P1 6372 643 82.4 globlastp 2468cephalotaxus|11v1|SRR064395X109174_T1 6373 643 82.24 glotblastn 2469pine|10v2|BX251480_P1 6374 643 82 globlastp 2470thellungiella_halophilum|11v1|BY816883 6375 643 81.8 globlastp 2471lettuce|12v1|DW050733_P1 6376 643 81.7 globlastp 2472maritime_pine|10v1|BX251480_P1 6377 643 81.5 globlastp 2473pigeonpea|11v1|SRR054580X131417_P1 6378 643 81.4 globlastp 2474arabidopsis|10v1|AT1G13180_P1 6379 643 81.3 globlastp 2475centaurea|gb166|EH715416 6380 643 81.3 globlastp 2476spruce|11v1|ES255727 6381 643 81.3 globlastp 2477arabidopsis_lyrata|09v1|JGIAL0013180_P1 6382 643 81.1 globlastp 2478thellungiella_parvulum|11v1|BY916884 6383 643 81.1 globlastp 2479pseudotsuga|10v1|SRR065119S0022026 6384 643 81.07 glotblastn 2480cacao|10v1|CU541357_T1 6385 643 80.67 glotblastn 2481b_rapa|11v1|DY025861_P1 6386 643 80.4 globlastp 2482canola|11v1|EE464832_P1 6387 643 80.4 globlastp 2483canola|11v1|EE446498_P1 6386 643 80.4 globlastp 2484canola|11v1|SRR329661.125838_P1 6388 643 80.4 globlastp 2485medicago|12v1|BF638396_T1 6389 643 80.37 glotblastn 2486b_rapa|11v1|DW998855_P1 6390 643 80.1 globlastp 2487sugarcane|10v1|CA065339_P1 6391 644 98.2 globlastp 2488maize|10v1|AI948098_P1 6392 644 91.9 globlastp 2489switchgrass|12v1|FE617860_P1 6393 644 88.8 globlastp 2490foxtail_millet|11v3|EC613703_P1 6394 644 87.8 globlastp 2491switchgrass|12v1|FE647199_T1 6395 644 86.96 glotblastn 2492millet|10v1|CD726439_T1 6396 644 85.22 glotblastn 2493cynodon|10v1|ES296885_P1 6397 644 83.5 globlastp 2494lovegrass|gb167|DN482827_P1 6398 644 81.7 globlastp 2495rice|11v1|BE039995_P1 6399 644 81.2 globlastp 2496barley|12v1|BI958660_P1 6400 644 80.5 globlastp 2497maize|10v1|AI001223_P1 645 645 100 globlastp 2498cenchrus|gb166|EB656881_P1 6401 645 99.3 globlastp 2499maize|10v1|AI855312_P1 6402 645 99.3 globlastp 2500millet|10v1|CD725181_P1 6401 645 99.3 globlastp 2501millet|10v1|EVO454PM072876_P1 6401 645 99.3 globlastp 2502wheat|12v3|CA485423 6402 645 99.3 globlastp 2503foxtail_millet|11v3|PHY7SI031386M_P1 6403 645 98.7 globlastp 2504lovegrass|gb167|DN481142_P1 6404 645 98.7 globlastp 2505rice|11v1|BE039677 6405 645 98.7 globlastp 2506 rice|11v1|BI794989_P16405 645 98.7 globlastp 2507 sugarcane|10v1|CA077917 6406 645 98.7globlastp 2508 switchgrass|gb167|DN140712 6407 645 98.7 globlastp 2509switchgrass|gb167|FE605366 6403 645 98.7 globlastp 2510switchgrass|gb167|FL716932 6403 645 98.7 globlastp 2511switchgrass|12v1|FL716932_P1 6403 645 98.7 globlastp 2512foxtail_millet|11v3|PHY7SI003223M_P1 6408 645 98 globlastp 2513sorghum|12v1|SB02G004110 6409 645 98 globlastp 2514sugarcane|10v1|CA070342 6410 645 98 globlastp 2515sugarcane|10v1|CA103755 6410 645 98 globlastp 2516switchgrass|gb167|DN146891 6408 645 98 globlastp 2517switchgrass|12v1|DN140712_P1 6408 645 98 globlastp 2518brachypodium|12v1|BRADI1G05670_P1 6411 645 96.7 globlastp 2519rice|11v1|AU031512 6412 645 94.7 globlastp 2520 rice|11v1|BI797945 6413645 94.7 globlastp 2521 switchgrass|12v1|FE605366_P1 6414 645 94.3globlastp 2522 brachypodium|12v1|BRADI1G55980_P1 6415 645 93.4 globlastp2523 barley|12v1|BF621440_P1 6416 645 92.8 globlastp 2524pseudoroegneria|gb167|FF348975 6417 645 92.8 globlastp 2525rye|12v1|BE494119 6418 645 92.8 globlastp 2526 rye|12v1|BG263876 6418645 92.8 globlastp 2527 rye|12v1|DRR001012.105054 6418 645 92.8globlastp 2528 rye|12v1|DRR001012.11360 6418 645 92.8 globlastp 2529rye|12v1|DRR001012.175741 6418 645 92.8 globlastp 2530rye|12v1|DRR001012.262220 6418 645 92.8 globlastp 2531wheat|12v3|BE402378 6418 645 92.8 globlastp 2532lovegrass|gb167|EH188825_T1 6419 645 92.8 globlastp 2533rye|12v1|DRR001012.405886 6420 645 92.76 glotblastn 2534rye|12v1|DRR001012.139413 6421 645 92.76 glotblastn 2535rye|12v1|DRR001012.146123 6422 645 92.76 glotblastn 2536rye|12v1|DRR001012.715073 6423 645 92.11 glotblastn 2537oat|11v1|CN816932_P1 6424 645 92.1 globlastp 2538oil_palm|11v1|EL930541_P1 6425 645 92.1 globlastp 2539rye|12v1|DRR001012.483052 6426 645 92.1 globlastp 2540maize|10v1|BM378337_T1 6427 645 91.45 glotblastn 2541fescue|gb161|DT6899971_Pa 6428 645 91.4 globlastp 2542ginger|gb164|DY359093_P1 6429 645 91.4 globlastp 2543ginger|gb164|DY361757_P1 6429 645 91.4 globlastp 2544oil_palm|11v1|EY409533_P1 6430 645 91.4 globlastp 2545phalaenopsis|11v1|CB031848_P1 6431 645 91.4 globlastp 2546phalaenopsis|11v1|SRR125771.1007801_P1 6432 645 91.4 globlastp 2547pineapple|10v1|CO730773_P1 6433 645 91.4 globlastp 2548poppy|11v1|SRR030259.107962_P1 6434 645 91.4 globlastp 2549poppy|11v1|SRR030259.156311_P1 6434 645 91.4 globlastp 2550poppy|11v1|SRR030259.236604_P1 6435 645 91.4 globlastp 2551poppy|11v1|SRR096789.118652_P1 6435 645 91.4 globlastp 2552cowpea|12v1|FF385204_P1 6436 645 90.8 globlastp 2553ipomoea_nil|10v1|BJ564090_P1 6437 645 90.8 globlastp 2554liriodendron|gb166|CK754187_P1 6438 645 90.8 globlastp 2555liriodendron|gb166|CK762830_P1 6438 645 90.8 globlastp 2556oat|11v1|GO594028_P1 6439 645 90.8 globlastp 2557peanut|10v1|EE127019_P1 6440 645 90.8 globlastp 2558peanut|10v1|ES703074_P1 6440 645 90.8 globlastp 2559platanus|11v1|SRR096786X109535_P1 6441 645 90.8 globlastp 2560platanus|11v1|SRR096786X116175_P1 6438 645 90.8 globlastp 2561platanus|11v1|SRR096786X144645_P1 6438 645 90.8 globlastp 2562poppy|11v1|FE967690_P1 6442 645 90.8 globlastp 2563phalaenopsis|11v1|SRR125771.1047942XX2_T1 6443 645 90.13 glotblastn 2564poppy|11v1|SRR030259.107504_T1 6444 645 90.13 glotblastn 2565acacia|10v1|FS5854542_P1 6445 645 90.1 globlastp 2566amorphophallus|11v2|SRR089351X100616_P1 6446 645 90.1 globlastp 2567aristolochia|10v1|SRR039082S0062615_P1 6447 645 90.1 globlastp 2568avocado|10v1|CK752507_P1 6448 645 90.1 globlastp 2569beet|12v1|BI643078XX2_P1 6449 645 90.1 globlastp 2570beet|12v1|BQ488142_P1 6450 645 90.1 globlastp 2571chelidonium|11v1|SRR084752X107444_P1 6447 645 90.1 globlastp 2572chelidonium|11v1|SRR084752X109905_P1 6447 645 90.1 globlastp 2573curcuma|10v1|DY385986_P1 6451 645 90.1 globlastp 2574eschscholzia|11v1|CK744958_P1 6447 645 90.1 globlastp 2575medicago|12v1|AW288024_P1 6452 645 90.1 globlastp 2576papaya|gb165|EX262807_P1 6453 645 90.1 globlastp 2577soybean|11v1|GLYMA10G36610 6454 645 90.1 globlastp 2578soybean|11v1|GLYMA10G36610T2_P1 6454 645 90.1 globlastp 2579trigonella|11v1|SRR066194X101951 6452 645 90.1 globlastp 2580trigonella|11v1|SRR066194X113780 6452 645 90.1 globlastp 2581trigonella|11v1|SRR066194X152074 6452 645 90.1 globlastp 2582tripterygium|11v1|SRR098677X113317 6455 645 90.1 globlastp 2583rye|12v1|DRR001012.217772 6456 645 89.54 glotblastn 2584olea|13v1|SRR014463X23649D1_P1 6457 645 89.5 globlastp 2585soybean|12v1|AI442769_P1 6458 645 89.5 globlastp 2586apple|11v1|CN879688_P1 6459 645 89.5 globlastp 2587aquilegia|10v2|DT741994_P1 6460 645 89.5 globlastp 2588aquilegia|10v2|JGIAC014479_P1 6461 645 89.5 globlastp 2589banana|12v1|ES433189_P1 6462 645 89.5 globlastp 2590banana|12v1|FF558491_P1 6462 645 89.5 globlastp 2591banana|12v1|FL667103_P1 6463 645 89.5 globlastp 2592cacao|10v1|CA794237_P1 6464 645 89.5 globlastp 2593cephalotaxus|11v1|SRR064395X100526_P1 6465 645 89.5 globlastp 2594cephalotaxus|11v1|SRR064395X102184_P1 6465 645 89.5 globlastp 2595cleome_spinosa|10v1|SRR015531S0000734_P1 6466 645 89.5 globlastp 2596cleome_spinosa|10v1|SRR015531S0011141_P1 6466 645 89.5 globlastp 2597cotton|11v1|AI1725541_P1 6464 645 89.5 globlastp 2598cotton|11v1|AI726016_P1 6464 645 89.5 globlastp 2599cotton|11v1|BE054022_P1 6464 645 89.5 globlastp 2600cotton|11v1|BQ406421_P1 6464 645 89.5 globlastp 2601cucumber|09v1|BI740218_P1 6467 645 89.5 globlastp 2602cyamopsis|10v1|EG980456_P1 6468 645 89.5 globlastp 2603euonymus|11v1|SRR070038X102341_P1 6469 645 89.5 globlastp 2604euonymus|11v1|SRR070038X104515_P1 6470 645 89.5 globlastp 2605euphorbia|11v1DV151673XX1_P1 6467 645 89.5 globlastp 2606flax|11v1|JG020740_P1 6471 645 89.5 globlastp 2607 flax|11v1|JG033539_P16471 645 89.5 globlastp 2608 fraxinus|11v1|SRR058827.135979_P1 6457 64589.5 globlastp 2609 fraxinus|11v1|SRR058827.143569_P1 6457 645 89.5globlastp 2610 fraxinus|11v1|SRR058827.166203_P1 6457 645 89.5 globlastp2611 gossypium_raimondii|12v1|AI725541_P1 6464 645 89.5 globlastp 2612gossypium_raimondii|12v1|AI726016_P1 6464 645 89.5 globlastp 2613gossypium_raimondii|12v1|BE054022_P1 6464 645 89.5 globlastp 2614gossypium_raimondii|12v1|BQ406421_P1 6464 645 89.5 globlastp 2615grape|11v1|GSVIVT01001168001_P1 6472 645 89.5 globlastp 2616grape|11v1|GSVIVT01017975001_P1 6472 645 89.5 globlastp 2617grape|11v1|GSVIVT01034582001q_P1 6472 645 89.5 globlastp 2618hornbeam|12v1|SRR364455.104726_P1 6473 645 89.5 globlastp 2619iceplant|gb164|BE035960_P1 6474 645 89.5 globlastp 2620ipomoea_batatas|10v1|CB330101_P1 6475 645 89.5 globlastp 2621ipomoea_nil|10v1|CJ746229_P1 6475 645 89.5 globlastp 2622liquorice|gb171|FS238680_P1 6476 645 89.5 globlastp 2623liquorice|gb171|FS244103_P1 6477 645 89.5 globlastp 2624melon|10v1|DV633021_P1 6467 645 89.5 globlastp 2625melon|10v1|DV633197_P1 6467 645 89.5 globlastp 2626nuphar|gb166|DT58550_P1 6468 645 89.5 globlastp 2627oat|11v1|CN815803_P1 6479 645 89.5 globlastp 2628 oat|11v1|GO590607_P16480 645 89.5 globlastp 2629 oil_palm|11v1|EY410764_P1 6481 645 89.5globlastp 2630 olea|11v1|SRR014463.15044 6457 645 89.5 globlastp 2631olea|11v1|SRR014463X.15044D1_P1 6457 645 89.5 globlastp 2632olea|11v1|SRR014463.15566 6457 645 89.5 globlastp 2633olea|11v1|SRR014463X.15566D1_P1 6457 645 89.5 globlastp 2634phyla|11v2|SRR099037X192733_P1 6482 645 89.5 globlastp 2635pigeonpea|11v1|SRR054580X101013_P1 6483 645 89.5 globlastp 2636pigeonpea|11v1|SRR054580X123056_P1 6484 645 89.5 globlastp 2637platanus|11v1|SRR096786X101919_P1 6485 645 89.5 globlastp 2638poplar|10v1|CN549329 6486 645 89.5 globlastp 2639poplar|13v1|CN549329_P1 6486 645 89.5 globlastp 2640poppy|11v1|FE964611_P1 6487 645 89.5 globlastp 2641 salvia|10v1|CV1628546488 645 89.5 globlastp 2642 soybean|11v1|GLYMA10G36880 6458 645 89.5globlastp 2643 soybean|12v1|GLYMA10G36880T2_P 6458 645 89.5 globlastp2644 soybean|11v1|GLYMA20G30730 6489 645 89.5 globlastp 2645soybean|12v1|GLYMA20G36880_P1 6489 645 89.5 globlastp 2646soybean|11v1|GLYMA20G30970 6490 645 89.5 globlastp 2647soybean|12v1|GLYMA20G30970_P1 6490 645 89.5 globlastp 2648spurge|gb161|DV151673 6467 645 89.5 globlastp 2649taxus|10v1|SRR032523S0032389 6465 645 89.5 globlastp 2650thalictrum|11v1|SRR096787X110776 6460 645 89.5 globlastp 2651tomato|11v1|BG125191 6491 645 89.5 globlastp 2652tripterygium|11v1|SRR087677X103895 6492 645 89.5 globlastp 2653tripterygium|11v1|SRR087677X115130 6493 645 89.5 globlastp 2654valeriana|11v1|SRR099039X100965 6494 645 89.5 globlastp 2655watermelon|11v1|B1740218 6467 645 89.5 globlastp 2656zostera|10v1|AM767488 6495 645 89.5 globlastp 2657apple|11v1|CN491562_T1 6496 645 89.47 glotblastn 2658cocao|10v1|CF973211_T1 6497 645 89.47 glotblastn 2659phyla|11v2|SRR099037X100375_T1 6498 645 89.47 glotblastn 2660fraxinus|11v1|SRR058827.122827_T1 6499 645 88.82 glotblastn 2661primula|11v1|SRR098679X130115_T1 6500 645 88.82 glotblastn 2662watermelon|11v1|DV633021 6501 645 88.82 glotblastn 2663xastorbean|12v1|EE258293_P1 6502 645 88.8 globlastp 2664centaurea|11v1|sRR346940.102154_P1 6503 645 88.8 globlastp 2665peach|gb157.2|AJ825789_P1 6504 645 88.8 globlastp 2666peach|gb157.2|DY633412_P1 6505 645 88.8 globlastp 2667prunus_mume|13v1|BU573849_P1 6504 645 88.8 globlastp 2668prunus_mume|13v1|CB821568_P1 6505 645 88.8 globlastp 2669acacia|10v1|GR483156_P1 6506 645 88.8 globlastp 2670amborella|12v3|CK749271_P1 6507 645 88.8 globlastp 2671apple|11v1|CN496803_P1 6504 645 88.8 globlastp 2672apple|11v1|CN883935_P1 6504 645 88.8 globlastp 2673aquilegia|10v2|JGIAC025912_P1 6508 645 88.8 globlastp 2674banana|12v1|FF560106_P1 6509 645 88.8 globlastp 2675bascilicum|10v1|DY322568_P1 6510 645 88.8 globlastp 2676bascilicum|10v1|DY335943_P1 6510 645 88.8 globlastp 2677bascilicum|10v1|DY336014_P1 6511 645 88.8 globlastp 2678blueberry|12v1|CF811672_P1 6512 645 88.8 globlastp 2679cannabis|12v1|JK501439_P1 6513 645 88.8 globlastp 2680cassava|09v1|CK644351_P1 6514 645 88.8 globlastp 2681cassava|09v1|DV442470_P1 6502 645 88.8 globlastp 2682cassava|09v1|DV447662_P1 6514 645 88.8 globlastp 2683castorbean|11v1|EE258293 6502 645 88.8 globlastp 2684castorbean|11v1|T24222 6514 645 88.8 globlastp 2685castorbean|11v1|T24222_P1 6514 645 88.8 globlastp 2686chestnut|gb170|SRR006295S00000282_P1 6515 645 88.8 globlastp 2687chestnut|gb170|SRR006295S00001374_P1 6516 645 88.8 globlastp 2688chickpea|11v1|DY475120 6517 645 88.8 globlastp 2689chickpea|11v1|GR393585 6517 645 88.8 globlastp 2690chickpea|13v2|GR393585_P1 6517 645 88.8 globlastp 2691clementine|11v1|CF504162_P1 6518 645 88.8 globlastp 2692cleome_gynandra|10v1|SRR015532S0005062_P1 6519 645 88.8 globlastp 2693cleome_gynandra|10v1|SRR015532S0018099_P1 6519 645 88.8 globlastp 2694cleome_spinosa|10v1|GR932171_P1 6519 645 88.8 globlastp 2695cleome_spinosa|10v1|SRR015531S0026352_P1 6519 645 88.8 globlastp 2696cryptomeria|gb166|BP174275_P1 6520 645 88.8 globlastp 2697cryptomeria|gb166|BW993048_P1 6520 645 88.8 globlastp 2698curcurbita|11v1|SRR091276X1_P1 6521 645 88.8 globlastp 2699cyamopsis|10v1|EG985540_P1 6522 645 88.8 globlastp 2700distylium|11v1|SRR065077X118115_P1 6523 645 88.8 globlastp 2701distylium|11v1|SRR065077X131771_P1 6524 645 88.8 globlastp 2702eucalyptus|11v2|CT984581_P1 6525 645 88.8 globlastp 2603eucalyptus|11v2|SRR001658X117_P1 6525 645 88.8 globlastp 2704euonymus|11v1|SRR070038X110227_P1 6526 645 88.8 globlastp 2705euonymus|11v1|SRR070038X110709_P1 6526 645 88.8 globlastp 2706euonymus|11v1|SRR070038X128756_P1 6527 645 88.8 globlastp 2707flax|11v1|EU830771_P1 6528 645 88.8 globlastp 2708 flax|11v1|JG017787_P16529 645 88.8 globlastp 2709 flax|11v1|JG028441_P1 6528 645 88.8globlastp 2710 flax|11v1|JG032308_P1 6528 645 88.8 globlastp 2711fraxinus|11v1|FR638309_P1 6530 645 88.8 globlastp 2712gerbera|09v1|AJ751009_P1 6531 645 88.8 globlastp 2713gerbera|09v1|AJ755281_P1 6531 645 88.8 globlastp 2714humulus|11v1|EX515858_P1 6513 645 88.8 globlastp 2715ipomoea_batatas|10v1|CB330422_P1 6532 645 88.8 globlastp 2716kiwi|gb166|FG396999_P1 6533 645 88.8 globlastp 2717kiwi|gb166|FG420418_P1 6533 645 88.8 globlastp 2718medicago|12v1|AA660242_P1 6534 645 88.8 globlastp 2719medicago|12v1|AL373560_P1 6534 645 88.8 globlastp 2720momordica|10v1|SRR071315S0006027_P1 6535 645 88.8 globlastp 2721momordica|10v1|SRR071315S0007312_P1 6535 645 88.8 globlastp 2722nuphar|gb166|CK752042_P1 6536 645 88.8 globlastp 2723oak|10v1|DB998847_P1 6515 645 88.8 globlastp 2724 oak|10v1|DN950342_P16516 645 88.8 globlastp 2725 orange|11v1|CF504162_P1 6518 645 88.8globlastp 2726 pepper|12v1|BM065411_P1 6537 645 88.8 globlastp 2727physcomitrella|10v1|AW509599_P1 6538 645 88.8 globlastp 2728physcomitrella|10v1|Z98068_P1 6539 645 88.8 globlastp 2729pigeonpea|11v1|GR468077_P1 6540 645 88.8 globlastp 2730poplar|10v1|AI162815 6541 645 88.8 globlastp 2731poplar|13v1|AI162815_P1 6541 645 88.8 globlastp 2732poppy|11v1|SRR096789.220194_P1 6542 645 88.8 globlastp 2733potato|10v1|AJ487428_P1 6543 645 88.8 globlastp 2764prunus|10v1|CB819415 6504 645 88.8 globlastp 2735 prunus|10v1|CB8215686505 645 88.8 globlastp 2736 rye|12v1|DRR001012.174607 6544 645 88.8globlastp 2737 salvia|10v1|CV165019 6545 645 88.8 globlastp 2738salvia|10v1|FE537147 6546 645 88.8 globlastp 2739scabiosa|11v1|SRR063723X108189 6547 645 88.8 globlastp 2740scabiosa|11v1|SRR063723X115009 6547 645 88.8 globlastp 2741sciadopitys|10v1|SRR065035S0007628 6548 645 88.8 globlastp 2742sequoia|10v1|SRR065044S1104606 6520 645 88.8 globlastp 2743sequoia|10v1|SRR065044S0029384 6520 645 88.8 globlastp 2744silene|11v1|GH293918 6549 645 88.8 globlastp 2745silene|11v1|SRR096785X106429 6550 645 88.8 globlastp 2746solanum_phureja|09v1|SPHBG125191 6543 645 88.8 globlastp 2747soybean|11v1|GLYMA02G08690 6551 645 88.8 globlastp 2748soybean|11v1|GLYMA02G08690_P1 6551 645 88.8 globlastp 2749tomato|11v1|AA824813 6552 645 88.8 globlastp 2750valeriana|11v1|SRR099039X101660 6553 645 88.8 globlastp 2751zamia|gb166|DY030861 6554 645 88.8 globlastp 2752centaurea|11v1|EH714043_P1 6503 645 88.8 globlastp 2753bean|12v2|CA897180_P1 6555 645 88.2 globlastp 2754 bean|12v2|CA903004_P16555 645 88.2 globlastp 2755 centaurea|11v1|EH741265_P1 6556 645 88.2globlastp 2756 centaurea|11v1|EH754977_P1 6556 645 88.2 globlastp 2757centaurea|11v1|SRR346938.107178_P1 6556 645 88.2 globlastp 2758centaurea|11v1|SRR346938.395477_P1 6556 645 88.2 globlastp 2759centaurea|11v1|SRR346941.103041_P1 6556 645 88.2 globlastp 2760nicotiana_benthamiana|12v1|CN746388_P1 6557 645 88.2 globlastp 2761olea|13v1|SRR014463X19320D1_P1 6558 645 88.2 globlastp 2762ambrosia|11v1|SRR346935.540449_P1 6559 645 88.2 globlastp 2763ambrosia|11v1|SRR346943.122145_P1 6559 645 88.2 globlastp 2764ambrosia|11v1|SRR346943.126555_P1 6559 645 88.2 globlastp 2765antirrhinumgb166|AJ558859_P1 6560 645 88.2 globlastp 2766aristolochia|10v|SRR039083S1002943_P1 6561 645 88.2 globlastp 2767arnica|11v1|SRR099034X101529_P1 6556 645 88.2 globlastp 2768arnica|11v1|SRR099034X101893_P1 6556 645 88.2 globlastp 2769arnica|11v1|SRR099034X11492_P1 6559 645 88.2 globlastp 2770artemisial|10v1|EY032434_P1 6556 645 88.2 globlastp 2771artemisial|10v1|EY032917_P1 6556 645 88.2 globlastp 2772artemisial|10v1|EY046218_P1 6556 645 88.2 globlastp 2773bean|12v1|CA903004 6555 645 88.2 globlastp 2774beech|11v1|SRR006293.11432_P1 6562 645 88.2 globlastp 2775beech|11v1|SRR006293.485_P1 6562 645 88.2 globlastp 2776centaurea|gb166|EH714043 6556 645 88.2 globlastp 2777centaurea|gb166|EH740958_P1 6556 645 88.2 globlastp 2778centaurea|gb166|EH740958 6556 645 88.2 globlastp 2779centaurea|gb166|EH752575_P1 6556 645 88.2 globlastp 2780centaurea|gb166|EH752575 6556 645 88.2 globlastp 2781ceratodon|10v1|AW086820_P1 6563 645 88.2 globlastp 2782ceratodon|10v1|SRR074890S031415_P1 6564 645 88.2 globlastp 2783ceratodon|10v1|SRR074890S0206507_P1 6564 645 88.2 globlastp 2784chickpea|11v1|SRR133517.201721 6565 645 88.2 globlastp 2785chickpea|11v1|SRR133517.201721_P1 6565 645 88.2 globlastp 2786chicorium|gb171|DT211776_P1 6556 645 88.2 globlastp 2787chicorium|gb171|EH698739_P1 6556 645 88.2 globlastp 2788cirsium|11v1|SRR346952.100954_P1 6556 645 88.2 globlastp 2789cirsium|11v1|SRR346952.1016079_P1 6556 645 88.2 globlastp 2790cirsium|11v1|SRR346952.1018181_P1 6556 645 88.2 globlastp 2791cirsium|11v1|SRR346952.110842_P1 6556 645 88.2 globlastp 2792cirsium|11v1|SRR346952.120716_P1 6556 645 88.2 globlastp 2793clementine|11v1|BE213468_P1 6566 645 88.2 globlastp 2794cowpea|12v1|FC460394_P1 6555 645 88.2 globlastp 2795cowpea|12v1|FF383542_P1 6555 645 88.2 globlastp 2796cucurbita|11v1|SRR091276X101659_P1 6567 645 88.2 globlastp 2797cucurbita|11v1|SRR091276X105539_P1 6567 645 88.2 globlastp 2798cyas|gb166|EX927238_P1 6568 645 88.2 globlastp 2799cyas|gb166|EX928133_P1 6569 645 88.2 globlastp 2800cynara|gb167|GE585921_P1 6556 645 88.2 globlastp 2801cynara|gb167|GE586256_P1 6556 645 88.2 globlastp 2802cynara|gb167|GE587349_P1 6556 645 88.2 globlastp 2803dandelion|10v1|DR398987_P1 6556 645 88.2 globlastp 2804dandelion|10v1|DR398999_P1 6556 645 88.2 globlastp 2805dandelion|10v1|DR399385_P1 6556 645 88.2 globlastp 2806dandelion|10v1|DY818871_P1 6556 645 88.2 globlastp 2807eggplant|10v1|FS000205_P1 6570 645 88.2 globlastp 2808eggplant|10v1|FS013827_P1 6571 645 88.2 globlastp 2809eucalyptus|11v2|CT988037_P1 6572 645 88.2 globlastp 2810euonymus|11v1|SRR070038X107698_P1 6573 645 88.2 globlastp 2811fagopyrum|11v1|SRR063689X100250_P1 6574 645 88.2 globlastp 2812fagopyrum|11v1|SRR063689X100675_P1 6574 645 88.2 globlastp 2813fagopyrum|11v1|SRR063703X118250_P1 6574 645 88.2 globlastp 2814fescue|gb161|DT690258_P1 6575 645 88.2 globlastp 2815flaveria|11v1|SRR149229.101618_P1 6559 645 88.2 globlastp 2816flaveria|11v1|SRR149229.106611_P1 6559 645 88.2 globlastp 2817flaveria|11v1|SRR149229.116457_P1 6556 645 88.2 globlastp 2818flaveria|11v1|SRR149229.128215_P1 6559 645 88.2 globlastp 2819flaveria|11v1|SRR149229.181354_P1 6559 645 88.2 globlastp 2820flaveria|11v1|SRR149229.472800_P1 6559 645 88.2 globlastp 2821flaveria|11v1|SRR149232.100137_P1 6559 645 88.2 globlastp 2822flaveria|11v1|SRR149232.110616_P1 6559 645 88.2 globlastp 2823flaveria|11v1|SRR149232.129420_P1 6559 645 88.2 globlastp 2824flaveria|11v1|SRR149232.129867_P1 6559 645 88.2 globlastp 2825flaveria|11v1|SRR149232.158991_P1 6556 645 88.2 globlastp 2826flaveria|11v1|SRR149232.286298_P1 6559 645 88.2 globlastp 2827flaveria|11v1|SRR149241.162170_P1 6559 645 88.2 globlastp 2828flaveria|11v1|SRR149241.174618_P1 6559 645 88.2 globlastp 2829flaveria|11v1|SRR149241.370637_P1 6576 645 88.2 globlastp 2830flaveria|11v1|SRR149242.140914_P1 6559 645 88.2 globlastp 2831fraxinus|11v1|SRR058827.17920_P1 6558 645 88.2 globlastp 2832ginseng|10v1|CN847306_P1 6577 645 88.2 globlastp 2833guizotia|10v1|GE552550_P1 6559 645 88.2 globlastp 2834guizotia|10v1|GE554321_P1 6559 645 88.2 globlastp 2835guizotia|10v1|GE557968_P1 6559 645 88.2 globlastp 2836hornbeam|12v1|SRR364455.106828_P1 6578 645 88.2 globlastp 2837jatropha|09v1|GH295800_P1 6579 645 88.2 globlastp 2838kiwi|gb166|FG417957_P1 6580 645 88.2 globlastp 2839lettuce|12v1|DW046230_P1 6581 645 88.2 globlastp 2840nasturtiium|11v1|GH163661_P1 6582 645 88.2 globlastp 2841orange|11v1|BE213468_P1 6566 645 88.2 globlastp 2842parthenium|10v1|GW784328_P1 6559 645 88.2 globlastp 2843parthenium|10v1|GW786047_P1 6559 645 88.2 globlastp 2844pepper|12v1|BM061831_P1 6583 645 88.2 globlastp 2845pepper|12v1|CA515799_P1 6584 645 88.2 globlastp 2846petunia|gb171|FN000831_P1 6557 645 88.2 globlastp 2847phyla|11v2|SRR099035X101639_P1 6585 645 88.2 globlastp 2848phyla|11v2|SRR099035X101664_P1 6586 645 88.2 globlastp 2849physcomitrella|10v1|AW497269_P1 6564 645 88.2 globlastp 2850physcomitrella|10v1|AW561228_P1 6564 645 88.2 globlastp 2851physcomitrella|10v1|AW599198_P1 6564 645 88.2 globlastp 2852plantago|11v2|SRR066373X114020_P1 6587 645 88.2 globlastp 2853plantago|11v2|SRR066373X134662_P1 6587 645 88.2 globlastp 2854poplar|10v1|AI163604 6588 645 88.2 globlastp 2855poplar|10v1|AI163604_P1 6588 645 88.2 globlastp 2856potato|10v1|BG098668_P1 6589 645 88.2 globlastp 2857rhizophoral|10v1|SRR005792S0001046 6590 645 88.2 globlastp 2858safflower|gb162|EL374295 6556 645 88.2 globlastp 2859safflower|gb162|EL392618 6556 645 88.2 globlastp 2860sarracenia|11v1|SRR192669.106537 6591 645 88.2 globlastp 2861senecio|gb170|DY658018 6592 645 88.2 globlastp 2862 sesame|12v1|BU6681846593 645 88.2 globlastp 2863 solanum_phureja|09v1|SPHAA824813 6594 64588.2 globlastp 2864 sunflower|12v1|CD847938 6559 645 88.2 globlastp 2865sunflower|12v1|CD849099 6559 645 88.2 globlastp 2866sunflower|12v1|CD850007 6556 645 88.2 globlastp 2867sunflower|12v1|CD851302 6559 645 88.2 globlastp 2868sunflower|12v1|CD851490 6559 645 88.2 globlastp 2869sunflower|12v1|CD851810 6559 645 88.2 globlastp 2870sunflower|12v1|CD857481 6559 645 88.2 globlastp 2871sunflower|12v1|CF078940 6556 645 88.2 globlastp 2872sunflower|12v1|CF079382 6556 645 88.2 globlastp 2873sunflower|12v1|CF091224 6559 645 88.2 globlastp 2874sunflower|12v1|DY908159 6559 645 88.2 globlastp 2875sunflower|12v1|DY918643 6559 645 88.2 globlastp 2876sunflower|12v1|DY920079 6559 645 88.2 globlastp 2877sunflower|12v1|DY924690 6559 645 88.2 globlastp 2878sunflower|12v1|DY957194 6559 645 88.2 globlastp 2879sunflower|12v1|EE640715 6559 645 88.2 globlastp 2880sunflower|12v1|EE650842 6556 645 88.2 globlastp 2881sunflower|12v1|SRR346950X101153 6559 645 88.2 globlastp 2882teal|1-v1|CV014409 6595 645 88.2 globlastp 2883 tobacco|gb162|BQ8428256596 645 88.2 globlastp 2884 tobacco|gb162|CV016244 6557 645 88.2globlastp 2885 tragopogon|10v1|SRR020205S0011374 6556 645 88.2 globlastp2886 tragopogon|10v1|SRR020205S0041653 6556 645 88.2 globlastp 2887triphysaria|10v1|EX982426 6597 645 88.2 globlastp 2888nicotiana_benthamiana|12v1|CN74325_P1 6557 645 88.2 globlastp 2889zostera|12v1|AM767488_T1 6598 645 88.16 glotblastn 2890chickpea|13v2|DY475120_T1 6599 645 88.16 glotblastn 2891nicotiana_benthamiana|12v1|EB447393_P1 6600 645 87.5 globlastp 2892switchgrass|12v1|FL947377_T1 6601 645 87.5 glotblastn 2893ambrosia|11v1|SRR346935.172586_P1 6602 645 87.5 glotblastn 2894ambrosia|11v1|SRR346943.110825_P1 6602 645 87.5 glotblastn 2895ambrosia|11v1|SRR346943.134260_P1 6602 645 87.5 glotblastn 2896ambrosia|11v1|SRR346943.175035_P1 6602 645 87.5 glotblastn 2897avocado|10v1|FD504015_P1 6603 645 87.5 globlastp 2898cannabis|12v1|EW700987_P1 6604 645 87.5 globlastp 2899cirsium|11v1|SRR349641.807871_P1 6605 645 87.5 globlastp 2900cucurbital|11v1|SRR091276X104052_T1 6606 645 87.5 glotblastn 2901cucurbital|11v1|SRR091277X114272_T1 6607 645 87.5 glotblastn 2902cycas|gb166|CB091000_P1 6608 645 87.5 globlastp 2903eggplant|10v1|FS001546_P1 6600 645 87.5 globlastp 2904fagopyrum|11v1|SRR063689X112242_P1 6609 645 87.5 globlastp 2905flaveria|11v1|SRR149229.124242_P1 6610 645 87.5 globlastp 2906flaveria|11v1|SRR149229.144508_T1 6611 645 87.5 glotblastn 2907flaveria|11v1|SRR149229.211249_T1 6612 645 87.5 glotblastn 2908flaveria|11v1|SRR149232.2133619_P1 6613 645 87.5 globlastp 2909flaveria|11v1|SRR149232.390430_T1 6614 645 87.5 glotblastn 2910guizotia|10v1|GE559342XX1_P1 6615 645 87.5 globlastp 2911humulus|11v1|EX515729_P1 6604 645 87.5 globlastp 2912lettuce|12v1|DW044998_P1 6616 645 87.5 globlastp 2913lettuce|12v1|DW103829_P1 6617 645 87.5 globlastp 2914lolium|10v1|DT672847_P1 6618 645 87.5 globlastp 2915lotus|09v1|BU494085_P1 6619 645 87.5 globlastp 2916marchantia|gb166|C95841_P1 6620 645 87.5 globlastp 2917marchantia|gb166|C96393_P1 6620 645 87.5 globlastp 2918monkeyflower|10v1|DV206181 6621 645 87.5 globlastp 2919monkeyflower|10v1|DV206181_P1 6621 645 87.5 globlastp 2920nasturtium|11v1|SRR032558.101013_P1 6622 645 87.5 globlastp 2921nicotiana_benthamiana|12v1|CN743096_P1 6623 645 87.5 globlastp 2922nicotiana_benthamiana|gb162|CN743096 6623 645 87.5 globlastp 2923nicotiana_benthamiana|12v1|CN744194_P1 6623 645 87.5 globlastp 2924nicotiana_benthamiana|gb162|CN744194 6623 645 87.5 globlastp 2925petunia|gb171|CV299836_P1 6623 645 87.5 globlastp 2926physcomitrella|10v1|AW599143_P1 6624 645 87.5 globlastp 2927podocarpus|10v1|SRR065014S0024080_P1 6625 645 87.5 globlastp 2928primula|11v1|FS229531_P1 6626 645 87.5 globlastp 2929primula|11v1|SRR098679X104291XX1_P1 6626 645 87.5 globlastp 2930primula|11v1|SRR098679X110646_P1 6626 645 87.5 globlastp 2931pteridium|11v1|GW574922 6627 645 87.5 globlastp 2932pteridium|11v1|SRR043594X117656 6627 645 87.5 globlastp 2933rose|12v1|BI977264 6628 645 87.5 globlastp 2934sarracenia|SRR192669.117714 6629 645 87.5 globlastp 2935sarracenia|SRR192669.129872 6630 645 87.5 globlastp 2936sciadopitys|10v1|SRR065035S00008606 6631 645 87.5 globlastp 2937senecio|gb170|CO553505 6632 645 87.5 globlastp 2938solanum_phureja|09v1|SPHBG123662 6623 645 87.5 globlastp 2939spikemoss|gb165|DN838212 6633 645 87.5 globlastp 2940spikemoss|gb165|FE433306 6633 645 87.5 globlastp 2941strawberry|11v1|CO38130 6628 645 87.5 globlastp 2942sunflower|12v1|BU671980 6634 645 87.5 globlastp 2943tobacco|gb162|CV020272 6600 645 87.5 globlastp 2944tobacco|gb162|DW001960 6623 645 87.5 globlastp 2945tobacco|gb162|EB677827 6600 645 87.5 globlastp 2946 tomato|11v1|BG1236626623 645 87.5 globlastp 2947 tragopogon|10v1|SRR020205S0036982 6635 64587.5 glotblastn 2948 triphysaria|10v|BM356875 6636 645 87.5 globlastp2949 triphysaria|10v|EX990722 6636 645 87.5 globlastp 2950utricularia|11v1|SRR094438.10133 6637 645 87.5 globlastp 2951dandelion|10v1|DY841132_T1 6635 645 86.84 glotblastn 2952poppy|11v1|SRR096789.252757_T1 6638 645 86.84 glotblastn 2953primula|11v1|SRR098679X209502_T1 6639 645 86.84 glotblastn 2954spurge|gb161|B1962078 6640 645 86.84 glotblastn 2955arabidopsis_lyrata|09v1|BQ834079_P1 6641 645 86.8 globlastp 2956arabidopsis_lyrata|09v1|JGIAL003533_P1 6641 645 86.8 globlastp 2957arabidopsis_lyrata|09v1|JGIAL022903_P1 6641 645 86.8 globlastp 2958arabidopsis|10v1|AT1G22780_P1 6641 645 86.8 globlastp 2959arabidopsis|10v1|AT1G34030_P1 6641 645 86.8 globlastp 2960arabidopsis|10v1|AT4G09800_P1 6641 645 86.8 globlastp 2961blueberry|12v1|CV190545_P1 6642 645 86.8 globlastp 2962blueberry|12v1|SRR353282X101939D1_P1 6642 645 86.8 globlastp 2963bupleurum|11v1|FG341930_P1 6643 645 86.8 globlastp 2964euphorbia|11v1|BI962078_P1 6644 645 86.8 globlastp 2965euphorbia|11v1|BP959355_P1 6645 645 86.8 globlastp 2966euphorbia|11v1|DV123555_P1 6646 645 86.8 globlastp 2967euphorbia|11v1|SSR098678X105620_P1 6646 645 86.8 globlastp 2968fern|gb171|BP911673_P1 6647 645 86.8 globlastp 2969fraxinus|11v1|SRR058827.100066_P1 6648 645 86.8 globlastp 2970lotus|09v1|LLBF177459_P1 6649 645 86.8 globlastp 2971nasturtium|11v1|sRR032558.108754_P1 6650 645 86.8 globlastp 2972nicotiana_benthamiana|gb162|CN743253 6651 645 86.8 globlastp 2973onion|12v1|CF439287_P1 6652 645 86.8 globlastp 2974onion|12v1|CF443333_P1 6652 645 86.8 globlastp 2975onion|12v1|SRR073446X121672D1_P1 6653 645 86.8 globlastp 2976onion|12v1|SRR073446X128281D1_P1 6653 645 86.8 globlastp 2977orobanche|10v|SRR023189S0008126_P1 6654 645 86.8 globlastp 2978orobanche|10v|SRR023189S0008932_P1 6655 645 86.8 globlastp 2979papaya|gb165|EX249656_P1 6656 645 86.8 globlastp 2980spurge|gb161|DV123555 6646 645 86.8 globlastp 2981abies|11v2|SRR098676X102010_P1 6657 645 86.2 globlastp 2982amsonia|11v1|SRR098688X101617_P1 6658 645 86.2 globlastp 2983arnica|11v1|SRR099034X156142_P1 6659 645 86.2 globlastp 2984cedrus|11v1|SRR065007X106236_P1 6660 645 86.2 globlastp 2985cedrus|11v1|SRR065007X155427_P1 6661 645 86.2 globlastp 2986gnetum|10v1|CB082487_P1 6662 645 86.2 globlastp 2987gnetum|10v1|DN955661_P1 6663 645 86.2 globlastp 2988guizotia|10v1|GE557973_P1 6664 645 86.2 globlastp 2989podocarpus|10v1|sRR065014S0007657 6665 645 86.2 globlastp 2990spruce|11v1|ES248603 6666 645 86.2 globlastp 2991 spruce|11v1|ES8545576666 645 86.2 globlastp 2992 spruce|11v1|EX350944 6666 645 86.2globlastp 2993 spruce|11v1|EX378591 6666 645 86.2 globlastp 2994spruce|11v1|EX409522 6666 645 86.2 globlastp 2995 spruce|11v1|EX4170226666 645 86.2 globlastp 2996 spruce|11v1|SRR064180X1121 6667 645 86.2globlastp 2997 spruce|11v1|SRR064180X150837 6666 645 86.2 globlastp 2998strawberry|11v1|DY673703 6668 645 86.2 globlastp 2999flaveria|11v1|SRR149229.238699XX1_P1 6669 645 85.6 globlastp 3000b_juncea|12v1|E6ANDIZ01AKYGR_P1 6670 645 85.53 glotblastn 3001spruce|11v1|EX411458XX2 6671 645 85.53 glotblastn 3002canola|11v1|CN729814_T1 — 645 85.53 glotblastn 3003b_juncea|12v1|E6ANDIZ01A0VSM_P1 6672 645 85.5 globlastp 3004b_juncea|12v1|E6ANDIZ01A15S3_P1 6672 645 85.5 globlastp 3005b_juncea|12v1|E6ANDIZ01A3RWZ_P1 6672 645 85.5 globlastp 3006b_juncea|12v1|E6ANDIZ01A9USI_P1 6672 645 85.5 globlastp 3007b_juncea|12v1|E6ANDIZ01AKACQ_P1 6672 645 85.5 globlastp 3008b_juncea|12v1|E6ANDIZ01AYF0F_P1 6672 645 85.5 globlastp 3009b_juncea|12v1|E6ANDIZ01ABZKR7_P1 6672 645 85.5 globlastp 3010b_oleracea|gb161|DY029753_P1 6672 645 85.5 globlastp 3011b_rapa|11v1|CD814622_P1 6672 645 85.5 globlastp 3012b_rapa|11v1|CD817700_P1 6672 645 85.5 globlastp 3013b_rapa|11v1|H74595_P1 6672 645 85.5 globlastp 3014 b_rapa|11v1|L38210_P16672 645 85.5 globlastp 3015 bruguiera|gb166|BP939636_P1 6673 645 85.5globlastp 3016 canola|11v1|CN725849_P1 6672 645 85.5 globlastp 3017canola|11v1|CN730875_P1 6672 645 85.5 globlastp 3018canola|11v1|CN735120_P1 6672 645 85.5 globlastp 3019canola|11v1|DW998748_P1 6672 645 85.5 globlastp 3020cirsium|11v1|SRR346952.42565_P1 6674 645 85.5 globlastp 3021flaveria|11v1|SRR149232.236591_P1 6675 645 85.5 globlastp 3022maritime_pine|10v1|BX665997_P1 6676 645 85.5 globlastp 3023pseudotsuga|10v1|SRR065119S0132532 6677 645 85.5 globlastp 3024radish|gb164|EV524681 6672 645 85.5 globlastp 3025 radish|gb164|EV5268916672 645 85.5 globlastp 3026 radish|gb164|EV535060 6672 645 85.5globlastp 3027 radish|gb164|EV543584 6672 645 85.5 globlastp 3028radish|gb164|EV544067 6672 645 85.5 globlastp 3029 radish|gb164|EW7222516672 645 85.5 globlastp 3030 radish|gb164|EX754584 6672 645 85.5globlastp 3031 radish|gb164|EY904167 6672 645 85.5 globlastp 3032rose|12v1|E586090 6678 645 85.5 globlastp 3033tabernaemontana|11v1|SRR098689X104258 6679 645 85.5 globlastp 3034thellungiella_parvulum|11v1|BE758566 6680 645 85.5 globlastp 3035thellungiella_parvulum|11v1|DN773306 6672 645 85.5 globlastp 3036vinca|11v1|SRR098690X108806 6681 645 85.5 globlastp 3037vinca|11v1|SRR098690X111525 6682 645 85.5 globlastp 3038vinca|11v1|SRR098690X125605 6683 645 85.5 globlastp 3039amsonia|11v1|SRR098688X108922_P1 6684 645 84.9 globlastp 3040b_oleraceal|gb161|DY025873_P1 6685 645 84.9 globlastp 3041bupleurum|11v1|SRR301254.12342_P1 6686 645 84.9 globlastp 3042canola|11v1|CN730635_P1 6685 645 84.9 globlastp 3043coffea|10v1|DV672412_P1 6687 645 84.9 globlastp 3044maritime_pine|10v1|BX249306_P1 6688 645 84.9 globlastp 3045onion|12v1|CF450522_P1 6689 645 84.9 globlastp 3046pine|10v2|AA556384_P1 6690 645 84.9 globlastp 3047 pine|10v2|AW010012_P16688 645 84.9 globlastp 3048 sunflower|12v1|DY956676 6691 645 84.9globlastp 3049 tabernaemontana|11V1|SRR098689X108058 6692 645 84.9globlastp 3050 zinnia|gb171|AU286978 6693 645 84.9 globlastp 3051rye|12v1|DRR001012.334772 6694 645 84.87 glotblastn 3052zostera|12v1|SRR057351X398302D1_T1 6695 645 84.52 glotblastn 3053nuphar|gb166|CD474613_P1 6696 645 84.5 globlastp 3054blueberry|12v1|SRR353282X21198D1_T1 6697 645 84.42 glotblastn 3055tripterygium|11v1|SRR098677X236892 6698 645 84.21 glotblastn 3056spruce|11v1|ES246342XX1 6699 645 84.2 globlastp 3057spruce|11v1|ES330372XX1 6699 645 84.2 globlastp 3058spruce|11v1|EX359965 6699 645 84.2 globlastp 3059 spruce|11v1|EX3932026699 645 84.2 globlastp 3060 spruce|11v1|GW72789XX2 6699 645 84.2globlastp 3061 thellungiella_halophilum|11v1|BE758566 6700 645 84.2globlastp 3062 thellungiella_halophilum|11v1|DN773306 6700 645 84.2globlastp 3063 jatropha|09v1|GO246712_P1 6701 645 84.2 globlastp 3064abies|11v2|SRR098676X116044_P1 6702 645 84.1 globlastp 3065catharanthus|11v1|EG560500_P1 6703 645 83.6 globlastp 3066pseudotsuga|10v1|SRR065119S0004272 6704 645 83.6 globlastp 3067sesame|12v1|BU668443 6705 645 83.6 globlastp 3068onoion|12v1|SRR073446X105670D1_T1 — 645 83.6 glotblastn 3069cotton|11v1|BM360106_P1 6706 645 83.55 globlastp 3070mesostigma|gb166|DN254443_P1 6707 645 82.89 globlastp 3071mesostigma|gb166|EC726940_P1 6707 645 82.89 globlastp 3072rye|12v1|DRR001012.104533 6708 645 82.89 glotblastn 3073clover|gb162|BB909024_P1 5709 645 82.2 globlastp 3074euphorbia|11v1|DV115835_P1 6710 645 82.2 globlastp 3075taxus|10v1|SRR065067S0068476 6711 645 81 globlastp 3076arnia|11v1|SRR099034X109741_T1 6712 646 80.24 glotblastn 3077sugarcane|10v1|CA084616 6713 646 97.6 globlastp 3078maize|10v1|BG320850_P1 6714 646 95.6 globlastp 3079cynodon|10v1|ES297594_P1 6715 646 92.2 globlastp 3080switchgrass|12v1|FE655376_P1 6716 646 91.7 globlastp 3081switchgrass|gb167|FE655376 6716 646 91.7 globlastp 3082millet|10v1|EVO454PM004269_P1 6717 646 91.3 globlastp 3083foxtail_millet|11v3|PHY7SI036909M_P1 6718 646 90.8 globlastp 3084switchgrass|gb167|FE626525 6719 646 90.3 globlastp 3085switchgrass|12v1|FE626525_P1 6719 646 90.3 globlastp 3086oat|11v1|GO597382_P1 6720 646 86.9 globlastp 3087rye|12v1|DRR001012.143939 6721 646 86.9 globlastp 3088pseudoroegneria|gb167|FF34390 6722 646 86.4 globlastp 3089rye|12v1|DRR001012.114293 6723 646 86.4 globlastp 3090rye|12v1|DRR001012.157386 6724 646 85.9 globlastp 3091wheat|12v3|CA620038 6725 646 85.1 globlastp 3092 rice|11v1|AT003489 6726646 85 globlastp 3093 barley|12v1|BF259164_P1 6727 646 84.5 globlastp3094 maize|10v1|CF650019_P1 6728 649 87.3 globlastp 3095maize|10v1|CF624028_P1 6729 650 87.6 globlastp 3096switchgrass|12v1|FL738039_P1 6730 650 84.8 globlastp 3097switchgrass|gb167|FL738039 6730 650 84.8 globlastp 3098foxtail_millet|11v3|pHY7SI031461M_P1 6731 650 82.8 globlastp 3099switchgrass|12v1|SRR187769.1032533_P1 6732 650 82.1 globlastp 3100sugarcane|10v1|AA577651 6733 651 99.3 globlastp 3101maize|10v1|AI941968_P1 6734 651 98.9 globlastp 3102maize|10v1|BE453848_P1 6735 651 98.9 globlastp 3103switchgrass|gb167|DN151885 6736 651 98.4 globlastp 3104foxtail_millet|11v3|pHY7SI001486M_P1 6737 651 97.9 globlastp 3105rice|11v1|BI806761 6738 651 97.7 globlastp 3106 barley|12v1|BE412764_P16739 651 93.8 globlastp 3107 oat|11v1|GO598315_P1 6740 651 93.8globlastp 3108 rye|12v1|DRR001012.116086 6739 651 93.8 globlastp 3109wheat|12v3|BI479831 6739 651 93.8 globlastp 3110brachypodium|12v1|BRADI2G10780_P1 6741 651 95.2 globlastp 3111cacao|10v1|CU477674_P1 6742 651 93.6 globlastp 3112castorbean|12v1|T14894_P1 6743 651 93.6 globlastp 3113clementine|11v1|CB291402_P1 6744 651 93.4 globlastp 3114poplar|10v1|BI125925 6745 651 93.4 globlastp 3115poplar|13v1|BI115925_P1 6745 651 93.4 globlastp 3116poplar|10v1|BU876008 6746 651 93.4 globlastp 3117poplar|13v1|BU876008_P1 6746 651 93.4 globlastp 3118prunus_mume|13v1|DY640313_P1 6747 651 93.2 globlastp 3119cotton|11v1|BG442148_P1 6748 651 93.2 globlastp 3120euphorbia|11v1|DV127470_P1 6749 651 93.2 globlastp 3121gossypium_raimondii|12v1|BG442148_P1 6748 651 93.2 globlastp 3122prunus|10v1|CN490622 6748 651 93.2 globlastp 3123grape|11v1|GSVIVT01025835001_P1 6750 651 92.9 globlastp 3124oil_palm|11v1|EY399362_P1 6751 651 92.9 globlastp 3125euonymus|11v1|SRR070038X10706_T1 6752 651 92.71 glotblastn 3126cotton|11v1|AI054691_P1 6753 651 92.7 globlastp 3127eucalyptus|11v2|AJ627799_P1 6754 651 92.7 globlastp 3128euonymus|11v1|SRR070038X109231_T1 6755 651 92.7 globlastp 3129nicotiana_benthamiana|12v1|EH623971_P1 6756 651 92.5 globlastp 3130amsonia|11v1|SRR098688X111941_P1 6757 651 92.5 globlastp 3131aristolochia|10v1|SRR039082S0015442_P1 6758 651 92.5 globlastp 3132monkeyflower|10v1|GR128396 6759 651 92.5 globlastp 3133monkeyflower|10v1|GR168616_P1 6759 651 92.5 globlastp 3134triphysaria|10v1|DR175197 6760 651 92.5 globlastp 3135phyla|11v2|SRR099037X112853_T1 6761 651 92.48 glotblastn 3136bean|12v1|CA898807_P1 6762 651 92.3 globlastp 3137nicotiana_benthamiana|12v1|EB428932_P1 6763 651 92.3 globlastp 3138ambrosia|11v1|SRR346935.110308_P1 6764 651 92.3 globlastp 3139ambrosia|11v1|SRR346935.164783_P1 6764 651 92.3 globlastp 3140banana|12v1|BBS742T3_P1 6765 651 92.3 globlastp 3141watermelon|11v1|AB029113 6766 651 92.3 globlastp 3142cucurbita|11v1|SRR091276X104159_P1 6767 651 92 globlastp 3143cassava|09v1|JGICASSAVA30380VALIDM1_P1 6768 651 91.8 globlastp 3144centaurea|11v1|EH717370_P1 6769 651 91.8 globlastp 3145centaurea|gb166|EH717370 6769 651 91.8 globlastp 3146cirsium|11v1|SRR346952.1001071_P1 6769 651 91.8 globlastp 3147cirsium|11v1|SRR346952.102960_P1 6770 651 91.8 globlastp 3148soybean|11v1|GLYMA03G00670 6771 651 91.8 globlastp 3149soybean|12v1|GLYMA03G00670_P1 6771 651 91.8 globlastp 3150sunflower|12v1CD855006 6772 651 91.8 globlastp 3151olea|13v1|SRR014463X47856D1_P1 6773 651 91.6 globlastp 3152cichorium|gb171|DT213455_P1 6774 651 91.6 globlastp 3153flaveria|11v1|sRR149229.125719_P1 6775 651 91.6 globlastp 3154gossypium_raimondii|12v1|AI054691_P1 6776 651 91.6 globlastp 3155lettuce|12v1DW058405_P1 6774 651 91.6 globlastp 3156pigeonpea|11v1|SRR054580X100816_P1 6777 651 91.6 globlastp 3157plantago|11v2|SRR066373X104231_P1 6778 651 91.6 globlastp 3158peanut|10v1|ES709894_T1 6779 651 91.57 glotblastn 3159flaveria|11v1|sRR149232.106043_T1 6780 651 91.34 glotblastn 3160soybean|11v1|GLYMA19G30100 6781 651 91.3 globlastp 3161soybean|12v1|GLYMA19G30100P1_P1 6781 651 91.3 globlastp 3162amborella|12v3|FD427212_P1 6782 651 91.1 globlastp 3163cassava|109v1|DV453089_P1 6783 651 91.1 globlastp 3164chestnut|gb170|SRR006295S0001803_P1 6784 651 91.1 globlastp 3165chickpea|11v1|SRR133517.102503 6785 651 91.1 globlastp 3166chickpea|13v2|SRR133517.102503_P1 6785 651 91.1 globlastp 3167oak|10v1|CU657187_P1 6784 651 91.1 globlastp 3168trigonella|11v1|SRR066194X138797 6786 651 91.1 globlastp 3169vinca|11v1|SRR098690X117502 6787 651 91.1 globlastp 3170potato|10v1|BF460323_P1 6788 651 90.9 globlastp 3171pseudotsuga|10v1|SRR065119S0025090 6789 651 90.9 globlastp 3172spruce|11v1|ES857077 6790 651 90.9 globlastp 3173 tomato|11v1|BG1339516791 651 90.9 globlastp 3174 arnica|11v1|SRR099034X115269_T1 6792 65190.89 glotblastn 3175 maritime_pine|10v1|AL750764_T1 6793 651 90.89glotblastn 3176 ambrosia|11v1|SRR346935.203357_P1 6794 651 90.7globlastp 3177 medicago|12v1|AL369598_P1 6795 651 90.7 globlastp 3178tripterygium|11v1|SRR098677X134816 6796 651 90.7 globlastp 3179valeriana|11v1|SRR099039X115968 6797 651 90.7 globlastp 3180abies|11v2|SRR098676X126999_T1 6798 651 90.66 glotblastn 3181poppy|11v1|SRR030259.100563_P1 6799 651 90.5 globlastp 3182onion|12v1|CF439443_P1 6800 651 90.4 globlastp 3183solanum_phureja|09v1|SPHBG133951 6801 651 90.4 globlastp 3184tomato|11v1|BG125980 6802 651 90.4 globlastp 3185vinca|11v1|SRR098690X102427 6803 651 90.4 globlastp 3186pine|10v2|AW064639_T1 6804 651 90.21 glotblastn 3187artemisia|10v1|GW330237_P1 6805 651 90.2 globlastp 3188millet|10v1|CD726085_P1 68006 651 90.2 globlastp 3189oak|10v1|CU656491_P1 6807 651 90.2 globlastp 3190solanum_phureja|09v1|SPHBG125980 6808 651 90.2 globlastp 3191phalaenopsis|11v1|CB033277XX1_T1 6809 651 90 glotblastn 3192strawberry|11v1|SRR034865S0022554 6810 651 90 globlastp 3193plantago|11v2|SRR066373X121450_T1 6811 651 89.98 glotblastn 3194sunflower|12v1|CD854950 6812 651 89.7 globlastp 3195aquilegia|10v2|DR926014_P1 6813 651 89.3 globlastp 3196silene|11v1|SRR096785X151884 6814 651 89.3 globlastp 3197soybean|11v1|GLYMA05G14170 6815 651 89.3 globlastp 3198soybean|12v1|GLYMA05G14170_P1 6815 651 89.3 globlastp 3199cephalotaxus|11v1|SRR064395X106738_P1 6816 651 89.1 globlastp 3200blueberry|12v1|SRR353282X16584D1_T1 6817 651 89.07 glotblastn 3201soybean|11v1|GLYMA19G17660 6818 651 88.8 globlastp 3202soybean|12v1|GLYMA19G17660_P1 6818 651 88.8 globlastp 3203solanum_phureja|09v1|SPHBQ518988 6819 651 88.6 globlastp 3204tomato|11v1|BQ518988 6820 651 88.4 globlastp 3205podocarpus|10v1|SRR065014S0020450_T1 6821 651 87.93 glotblastn 3206beech|11v1|SRR006293.18332_P1 6822 651 87.5 globlastp 3207pseudoroegneria|gb167|FF343185 6823 651 87.2 globlastp 3208ceratodon|10v1|SRR074890S0017271_P1 6824 651 87 globlastp 3209orange|11v1|CB291402_P1 6825 651 87 globlastp 3210spikemoss|gb165|FE459932 6826 651 87 globlastp 3211gnetum|10v1|SRR064399S0002380_P1 6827 651 86.8 globlastp 3212physcomitrella|10v1|BJ948943_P1 6828 651 86.8 globlastp 3213physcomitrella|10v1|BY951095_P1 6829 651 86.6 globlastp 3214sesame|12v1|SRR197996S268919 6830 651 86.6 globlastp 3215physcomitrella|10v1|BY959963_P1 6831 651 86.3 globlastp 3216safflower|gb162|EL373776 6832 651 86.3 globlastp 3217thellungiella_halophilum|11v1|DN776355 6833 651 85.4 globlastp 3218switchgrass|12v1|DN151885_P1 6834 651 84.7 globlastp 3219centaurea|11v1|EH755352_P1 6835 651 84.3 globlastp 3220arabidopsis_lyrata|09v1|JGIAL013630_P1 6836 651 84.3 globlastp 3221canola|11v1|DR697811_P1 6837 651 84.3 globlastp 3222pteridium|11v1|SRR043294X105728 6838 651 84.3 globlastp 3223radish|gb164|EV525450 6839 651 84.3 globlastp 3224thellungiella_parvulum|11v1|DN776355 6840 651 84.3 globlastp 3225b_juncea|12v1|E6ANDIZ01A2O2F_P1 6841 651 84.1 globlastp 3226b_rapa|11v1|CD836540_P1 6842 651 84.1 globlastp 3227arabidopsis|10v1AT2G26990_P1 6843 651 83.8 globlastp 3228b_rapa|11v1|BQ791498_P1 6844 651 83.8 globlastp 3229canola|11v1|EE405876_P1 6844 651 83.8 globlastp 3230zostera|10v1|SRR057351S0016657 6845 651 83.48 glotblastn 3231zostera|12v1|SRR057351X16656D1_P1 6846 651 83.4 globlastp 3232canola|11v1|CN829705_P1 6847 651 82.9 globlastp 3233apple|11v1|CN903733_T1 6848 651 82.77 glotblastn 3234pine|10v2|DR181028_P1 6849 651 83.2 globlastp 3235b_juncea|12v1|E6ANDIZ01BK6Y2_P1 6850 651 82.2 globlastp 3236canola|11v1|ES956907_P1 6851 651 82.2 globlastp 3237phyla|11v2|SRR099035X113355_P1 6852 651 81.8 globlastp 3238maize|10v1|AI396488_P1 6853 652 95.1 globlastp 3239foxtail_millet|11v3|EC613545_P1 6854 652 91.5 globlastp 3240switchgrass|12v1|FL722154_P1 6855 652 91.4 globlastp 3241millet|10v1|EVO454PM005730_P1 6856 652 88.2 globlastp 3242rie|11v1|AU056143_P1 6857 652 84.4 globlastp 3243brachypodium|12v1|BRADI2G11490_P1 6858 652 81.3 globlastp 3244maize|10v1|CO521347_P1 6859 652 86.9 globlastp 3245maize|10v1|GRMZM2G013481T01_P1 6860 652 82.7 globlastp 3246foxtail_millet|11v3|PHY7SI001170M_P1 6861 653 81.7 globlastp 3247switchgrass|gb167|FE597776 6862 653 81.7 globlastp 3248sugarcane|10v1|CA076988 6863 654 94.3 globlastp 3249switchgrass|gb167|DN143458 6864 654 87.3 globlastp 3250switchgrass|gb167|FL790768 6865 654 87.3 globlastp 3251switchgrass|12v1|DN143458_P1 6864 654 87.3 globlastp 3252maize|10v1|AI637145_P1 6866 654 86.9 globlastp 3253foxtail_millet|11v3|PHY7SI010718M_P1 6867 654 86.1 globlastp 3254millet|10v1|EVO454PM013400_P1 6868 654 80.9 globlastp 3255sugarcane|10v1|CA110549 6869 655 98.7 globlastp 3256switchgrass|12v1|FL936357_P1 6870 655 96.2 globlastp 3257maize|10v1|CF627898_P1 6871 655 93.8 globlastp 3258cynodon|10v1|ES298778_P1 6872 655 93.7 globlastp 3259sugarcane|10v1|CA116416 6873 655 93.7 globlastp 3260switchgrass|12v1|FE602476_P1 6874 655 92.8 globlastp 3261switchgrass|gb167|FE602476 6874 655 92.8 globlastp 3262millet|10v1|PMSLX0015697D1_P1 6875 655 92.4 globlastp 3263sorghum|12v1|SB03G001890 6876 655 92.4 globlastp 3264wheat|12v3|CA486543 6876 655 92.4 globlastp 3265 maize|10v1|BG267487_P16877 655 91.1 globlastp 3266 foxtail_millet|11v3|PHY7SI003590M_P1 6878655 89.9 globlastp 3267 sugarcane|10v1|CA300742 6879 655 88.61glotblastn 3268 switchgrass|12v1|FL770757_P1 6880 655 88.6 globlastp3269 switchgrass|12v1|DN144939_P1 6880 655 88.6 globlastp 3270switchgrass|gb167|DN144939 6880 655 88.6 globlastp 3271switchgrass|12v1|FL935819_P1 6880 655 88.6 globlastp 3272switchgrass|gb167|FL770757 6880 655 88.6 globlastp 3273rice|11v1|BX898318 6881 655 88.5 globlastp 3274foxtail_millet|11v3|SICRP041315_P1 6882 655 87.7 globlastp 3275rice|11v1|BM038666 6883 655 87.3 globlastp 3276 barley|12v1|BE193278_P16884 655 84.7 globlastp 3277 rye|12v1|dRR01012.106228 6885 655 84.7globlastp 3278 wheat|12v3|BE401123 6886 655 84.7 globlastp 3279oat|11v1|SRR020741.357758_T1 6887 655 81.61 glotblastn 3280brachypodium|12v1|BRADI2G06930_P1 6888 655 80.5 globlastp 3281sugarcane|10v1|CA154279 6889 656 82.2 globlastp 3282sugarcane|10v1|CA065678 6890 657 97 globlastp 3283maize|10v1|AI932203_P1 6891 657 96.7 globlastp 3284foxtail_millet|11v3|EC611910_TP1 6892 657 92.7 glotblastn 3285switchgrass|gb167|DN150553 6893 657 92.3 globlastp 3286switchgrass|12v1|DN150553_P1 6894 657 92 globlastp 3287millet|10v1|EVO454PM011444_P1 6895 657 91.8 globlastp 3288switchgrass|12v1|DN151303_T1 6896 657 91.49 glotblastn 3289rice|11v1|AU057478 6897 657 88.9 globlastp 3290brachypodium|12v1|BRADI2G19326_P1 6898 657 88 globlastp 3291barley|12v1|BI947807_P1 6899 657 86.6 globlastp 3292rye|12v1|DRR001012.141872 6900 657 86.4 globlastp 3293rye|12v1|DRR001012.318622 6901 657 86.39 glotblastn 3294rye|12v1|DRR001012.254493 6902 657 84.8 globlastp 3295rye|12v1|DRR001012.258882 6903 657 82.72 glotblastn 3296brachypodium|12v1|BRADI2G49280_P1 6904 657 82.5 globlastp 3297rye|12v1|BE63707 6905 657 91.9 globlastp 3298 rice|11v1|AU082277 6906657 81.4 globlastp 3299 maize|10v1|AI1600686_P1 6907 657 81.1 globlastp3300 rye|12v1|DRR001012.142448 6908 657 80.84 glotblastn 3301switchgrass|12v1|FL712047_P1 6909 657 80.8 globlastp 3302barley|12v1|BF261099_P1 6910 657 80.6 globlastp 3303switchgrass|12v1|FL691024_P1 6911 657 80.4 globlastp 3304switchgrass|gb167|FL691024 6911 657 80.4 globlastp 3305wheat|12v3|BF474139 6912 657 80.4 globlastp 3306rye|12v1|DRR001012.142799 6913 657 80.3 globlastp 3307sorghum|12v1|SB03G034060 6914 657 80.27 glotblastn 3308millet|10v1|EVO454PM03766_P1 6915 657 80.2 globlastp 3309sugarcane|10v1|CA074352 6916 658 98.3 globlastp 3310switchgrass|12v1|DN151653_P1 6917 658 96.2 globlastp 3311switchgrass|12v1|FE628285_P1 6918 658 96.1 globlastp 3312foxtail_millet|11v3|PHY7SI005731M_P1 6919 658 95.9 globlastp 3313maize|10v1|AW066998_P1 6920 658 95.1 globlastp 3314switchgrass|gb167|FE630258 6921 658 93.7 globlastp 3315millet|10v1|EVO454PM002624_P1 6922 658 93 globlastp 3316rice|11v1|BE229866 6923 658 92.2 globlastp 3317brachypodium|12v1|BRADI1G46230_P1 6294 658 89.9 globlastp 3318rye|12v1|DRR001012.104803 6925 658 89 globlastp 3319rye|12v1|DRR001012.109533 6925 658 89 globlastp 3320 wheat|12v3|BE5158016926 658 86.7 globlastp 3321 rye|12v1|DRR001012.14078 6927 658 85.2globlastp 3322 wheat|12v3|B429144_P1 6928 658 80.8 globlastp 3323foxtail_millet|11v3|PHY7SI005798M_P1 6929 659 82.2 globlastp 3324maize|10v1|SRR014549S0022069_P1 6930 659 81.7 globlastp 3325cowpea|12v1|FG938043_P1 6931 660 86.1 globlastp 3326soybean|11v1|GLYMA19G38100 6932 660 85.2 globlastp 3327soybean|12v1|GLYMA19G38100_P1 6932 660 85.2 globlastp 3328bean|12v2|CA915339_P1 6933 660 84.3 globlastp 3329 bean|12v2|CA9153396933 660 84.3 globlastp 3330 lotus|09v1|GO019465_P1 6934 660 83.3globlastp 3331 pigeonpea|11v1|SRR054580X10059_P1 6935 660 83.3 globlastp3332 chickpea|13v1|SRR133517.575663_P1 6936 660 80.6 globlastp 3333peanut|10v1|GO342698_T1 6937 660 80.56 glotblastn 3334soybean|11v1|GLYMA04G43300 6938 661 97.8 globlastp 3335soybean|12v1|GLYMA19G43300_P1 6938 661 97.8 globlastp 3336pigeonpea|11v1|SRR054580X29123_P1 6939 661 97 globlastp 3337chickpea|11v1|SRR133517.1533 6940 661 95.6 globlastp 3338chickpea|13v1|SRR133517.224160_P1 6940 661 95.6 globlastp 3339cowpea|12v1|FF549703_P1 6941 661 95.6 globlastp 3340lotus|09v1|LLBI420463_P1 6942 661 95.6 globlastp 3341bean|12v2|SRR001334.164203_P1 6943 661 94.1 globlastp 3342liqurice|gb171|FS242023_P1 6944 661 94.1 globlastp 3343medicago|12v1|AL379894_P1 6945 661 94.1 globlastp 3344peanut|10v1|EE126206_P1 6946 661 94.1 globlastp 3345trigonella|11v1|SRR066194X108510 6945 661 94.1 globlastp 3346pea|11v1|FG535587_P1 6947 661 90.4 globlastp 3347 cacao|10v1|CU475574_P16948 661 88.1 globlastp 3348 castorbean|12v1|XM_002513790_P1 6949 66187.4 globlastp 3349 chestnut|gb170|SRR006295S0058141_P1 6950 661 87.4globlastp 3350 solanum_phureja|SPHBI210247 6951 661 86.8 globlastp 3351tobacco|gb162|DV157543 6952 661 86.8 globlastp 3352beech|11v1|SRR006293.11243_P1 6953 661 86.7 globlastp 3353clementine|11v1|CX640424_P1 6954 661 86.7 globlastp 3354cleome_spinosa|10v1|GR931729_P1 6955 661 86.7 globlastp 3355flax|11v1|JG022460_P1 6956 661 86.7 globlastp 3356 flax|11v1|JG026610_P16957 661 86.7 globlastp 3357 oak|10v1|FP042586_P1 6958 661 86.7globlastp 3358 tomato|11v1|BI210247 6959 661 86 globlastp 3359cucurbita|11v1|SRR091276X115885_T1 6960 661 85.93 glotblastn 3360cassava|09v1|JGICASSAVA36154VALIDMI_P1 6961 661 85.9 globlastp 3361cotton|11v1|DR459043_P1 6962 661 85.9 globlastp 3362cucumber|09v1|AM720808_P1 6963 661 85.9 globlastp 3363euphorbia|11v1|SRR098678X105121_P1 6964 661 85.9 globlastp 3364ipomoea_nil|10v1|BJ557095_P1 6965 661 85.9 globlastp 3365melon|10v1|AM720808_P1 6966 661 85.9 globlastp 3366orange|11v1|CX640424_P1 6967 661 85.9 globlastp 3367watermelon|11v1|AM740273 6966 661 85.9 globlastp 3368apple|11v1|CN488504_P1 6968 661 85.2 globlastp 3369cleome_gynandra|10v1|SRR015532S0011206_P1 6969 661 85.2 globlastp 3370cotton|11v1|DT569102XX2_P1 6970 661 85.2 globlastp 3371cucurbita|11v1|SRR091276X118557_P1 6971 661 85.2 globlastp 3372euonymus|11v1|SRR070038X125307_P1 6972 661 85.2 globlastp 3373gossypium_raimondii|12v1|DR459043_P1 6973 661 85.2 globlastp 3374nasturtium|11v1|SRR032558.107200_P1 6974 661 85.2 globlastp 3375strawberry|11v1|EX663963 6975 661 85.2 globlastp 3376tripterygium|11v1|SRR098677X125486 6976 661 85.2 globlastp 3377flaveria|11v1|SRR149244.84482_T1 6977 661 84.44 glotblastn 3378jatropha|09v1|GO247666_T1 6978 661 84.44 glotblastn 3379artemisia|10v1|EY070433_P1 6979 661 84.4 globlastp 3380artemisia|10v1|SRR019254S0100726_P1 6979 661 84.4 globlastp 3381cannabis|12v1|SOLX00051864_P1 6980 661 84.4 globlastp 3382chelidonium|11v1|SRR074752X19452_P1 6981 661 84.4 globlastp 3383cichorium|gb171|EH703976_P1 6982 661 84.4 globlastp 3384cotton|11v1|BG444423_P1 6983 661 84.4 globlastp 3385cotton|11v1|DW238196_P1 6984 661 84.4 globlastp 3386eucalyptus|11v2|SRR001658X6931_P1 6985 661 84.4 globlastp 3387flaveria|11v1|SRR149229.247145_P1 6986 661 84.4 globlastp 3388gossypium_raimondii|12v1|BG444423_P1 6983 661 84.4 globlastp 3389gossypium_raimondii|12v1|DT569102_P1 6987 661 84.4 globlastp 3390grape|11v1|GSVIVT01008943001_P1 6988 661 84.4 globlastp 3391humulus|11v1|EX519738_P1 6980 661 84.4 globlastp 3392poplar|10v1|AI165677 6989 661 84.4 globlastp 3393poplar|13v1|AI165677_P1 6989 661 84.4 globlastp 3394poppy|11v1|FE964787_P1 6990 661 84.4 globlastp 3395poppy|11v1|SRR030259.167215_P1 6990 661 84.4 globlastp 3396poppy|11v1|SRR096789.113148_P1 6990 661 84.4 globlastp 3397thalictrum|11v1|sRR096787X154209 6991 661 84.4 globlastp 3398nicotiana_benthamiana|gb162|CN746865 6992 661 83.8 globlastp 3399amsonia|11v1|SRR098688X105761_P1 6993 661 83.7 globlastp 3400b_juncea|12v1|E6ANDIZ01C0JEQ_P1 6994 661 83.7 globlastp 3401b_juncea|12v1|E6ANDIZ01ENV7R_P1 6994 661 83.7 globlastp 3402b_rapa|11v1AM385563_P1 6994 661 83.7 globlastp 3403canola|11v1|EE477425_P1 6994 661 83.7 globlastp 3404catharanthus|11v1|SRR098691X103316_P1 6995 661 83.7 globlastp 3405cirsium|11v1|SRR346952.102640_P1 6996 661 83.7 globlastp 3406dandelion|10v1|DY836116_P1 6997 661 83.7 globlastp 3407eschscholzia|11v1|SRR014116.124609_P1 6998 661 83.7 globlastp 3408euonymus|11v1|SRR070038X103789_P1 6999 661 83.7 globlastp 3409euonymus|11v1|SRR070038X443976_P1 7000 661 83.7 globlastp 3410euphorbia|11v1|DV126650_P1 7001 661 83.7 globlastp 3410spurge|gb161|DV126650 7001 661 83.7 3411fagopyrum|11v1|SRR063703X103971_T1 7002 661 83.7 glotblastn 3412kiwi|gb166|FG10748_P1 7003 661 83.7 globlastp 3413lettuce|12v1|DW050122_P1 7004 661 83.7 globlastp 3414radish|gb164|EV529131 6994 661 83.7 globlastp 3415 radish|gb164|EV5442117005 661 83.7 globlastp 3416 radish|gb164|EW725749 6994 661 83.7globlastp 3417 radish|gb164|EX770883 6994 661 83.7 globlastp 3418radish|gb164|FD970891 6994 661 83.7 globlastp 3419sunflower|12v1|EL487785 7006 661 83.7 globlastp 3420valeriana|11v1|SRR099039X124935 7007 661 83.7 globlastp 3421valeriana|11v1|SRR099039X36534 7007 661 83.7 globlastp 3422vinca|11v1|SRR098690X205121 7008 661 83.7 globlastp 3423centaurea|11v1|EH737429_P1 7009 661 83 globlastp 3424centaurea|11v1|EH785456_P1 7009 661 83 globlastp 3425peach|gb157.2|AJ823102_P1 7010 661 83 globlastp 3426ambrosia|11v1|SRR346943.179652_P1 7011 661 83 globlastp 3427arabidopsis_lyrata|09v1|JGIAL003809_P1 7012 661 83 globlastp 3428arabidopsis|10v1|AT1G36980_P1 7012 661 83 globlastp 3429aristolochia|10v1|FD752910_P1 7013 661 83 globlastp 3430b_oleracea|gb161|AM385563_P1 7014 661 83 globlastp 3431blueberry|12v1|sRR353282X16196D1_P1 7015 661 83 globlastp 3432centaurea|gb166|EH737429 7009 661 83 globlastp 3433cirsium|11v1|SRR346952.1027758_P1 7009 661 83 globlastp 3434cleome_spinosa|10v1|GR930922_P1 7016 661 83 globlastp 3435euonymus|11v1|SRR070038X133921_P1 7017 661 83 globlastp 3436flaveria|11v1|SRR149245.189891_P1 7018 661 83 globlastp 3437guizotia|10v1|GE553450_P1 7019 661 83 globlastp 3438platanus|11v1|SRR096786X127458_P1 7020 661 83 globlastp 3439poplar|10v1|BU891793 7021 661 83 globlastp 3440 poplar|13v1|BU891793_P17022 661 83 globlastp 3441 prunus|10v1|CB819061 7010 661 83 globlastp3442 scabiosa|11v1|SRR063723X101694 7023 661 83 globlastp 3443tabernaemontana|11v1|SRR098689X101508 7024 661 83 globlastp 3444thellungiella_halophilum|11v1|BY807852 7025 661 83 globlastp 3445thellungiella_parvulum|11v1|BY807852 7026 661 83 globlastp 3446utricularia|11v1|SRR094438.100900 7027 661 83 globlastp 3447walnuts|gb166|V198107 7028 661 83 globlastp 3448fagopyrum|11v1|SRR063689X106595_T1 7029 661 82.96 glotblastn 3449arnica|11v1|SRR099034X269189_T1 — 661 82.96 glotblastn 3450sunflower|12v1|EE635394 7030 661 82.4 globlastp 3451ambrosia|11v1|SRR346935.521531_T1 7031 661 82.22 glotblastn 3452sunflower|12v1|DY953735 7032 661 82.22 glotblastn 3453antirrhinm|gb166|AJ560024_P1 7033 661 82.2 glotblastn 3454cynara|gb167|GE590748_P1 7034 661 82.2 globlastp 3455flaveria|11v1|SRR149229.213310_P1 7035 661 82.2 globlastp 3456plantago|11v2|SRR066373X102369_P1 7036 661 82.2 globlastp 3457rhizophora|10v1|SRR005793S0014453 7037 661 82.2 globlastp 3458silene|11v1|GH292314 7038 661 82.2 globlastp 3459silene|11v1|SRR096785X134112 7039 661 82.2 globlastp 3460teal|10v1|GE650230 7040 661 82.2 globlastp 3461 beet|12v1|CK136709_P17041 661 81.5 globlastp 3462 radish|gb164|EW716990 7042 661 81.5globlastp 3463 rose|12v1|SRR397984.19572 7043 661 81.5 globlastp 3464sesame|12v1|SESI2V1242066 7044 661 81.5 globlastp 3465safflower|gb162|EL405269 7045 661 81.48 glotblastn 3466phyla|11v2|SRR099037X142101_T1 7046 661 80.74 glotblastn 3467triphysaria|10v1|SRR023500S0010336 7047 661 80.74 glotblastn 3468olea|13v1|SRR014464X2072D1_P1 7048 661 80.7 globlastp 3469amorphophallus|11v2|SRR089351X138647_P1 7049 661 80.7 globlastp 3470monkeyflower|10v1|GR037920 7050 661 80.7 globlastp 3471monkeyflower|12v1|GR037921_P1 7050 661 80.7 globlastp 3472orobanche|10v1|SRR023189S0020330_P1 7051 661 80.7 globlastp 3473phyla|11v2|SRR099035X106217_P1 7052 661 80.7 globlastp 3474platanus|11v1|SRR096786X123476_P1 7053 661 80.7 globlastp 3475fraxinus|11v1|SRR058827.107115_P1 7054 661 80.3 globlastp 3476bruguiera|gb166|BP945233_P1 7055 661 80 globlastp 3477primula|11v1|SRR098679X141285_T1 7056 661 80 glotblastn 3478sesame|12v1|SESI12V1255028 7057 661 80 globlastp 3479soybean|11v1|GLYMA08G22510 7058 662 82.8 globlastp 3480soybean|12v1|GLYMA08G22510_P1 7058 662 82.8 globlastp 3481soybean|11v1|GLYMA15G05100 7059 663 96.5 globlastp 3482soybean|12v1|GLYMA15G05100_P1 7059 663 96.5 globlastp 3483pigeonpea|11v1|SRR054580X121984_P1 7060 663 91.3 globlastp 3484cowpea|12v1|FF395717_P1 7061 663 89.6 globlastp 3485bean|12v2|CA913094_P1 7062 663 88.9 globlastp 3486 bean|12v2|CA9130947063 663 86.1 globlastp 3487 medicago|12v1|AW685080_P1 7064 663 85.4globlastp 3488 peanut|10v1|ES720983_P1 7065 663 85.4 globlastp 3489trigonella|11v1|SRR066194X150097 7066 663 84.7 globlastp 3490chickpea|11v1|SRR133517.290513 7067 663 83.7 globlastp 3491chickpea|13v1|SRR133517.290513_P1 7067 663 83.7 globlastp 3492cacao|10v1|CA796237_P1 7068 663 80.2 globlastp 3493soybean|11v1|GLYMA07G09230 7069 664 99 globlastp 3494soybean|12v1|GLYMA07G09320_P1 7069 664 99 globlastp 3495cowpea|12v1|FF384990_P1 7070 664 95.6 globlastp 3496pigeonpea|11v1|GR466312_P1 7071 664 95.1 globlastp 3497bean|12v2|CA896990_P1 7072 664 91.3 globlastp 3498liquorice|gb171|FS245051_P1 7073 664 91.3 globlastp 3499chickpea|13v2|SRR133517.127065_P1 7074 664 89.8 globlastp 3500medicago|12v1|BF645221_P1 7075 664 88.8 globlastp 3501pigeonpea|11v1|SRR054580X311299_P1 7076 664 88.8 globlastp 3502trigonella|11v1|SRR066194X167645 7077 664 88.8 globlastp 3503chickpea|11v1|GR408372 7078 664 88.3 globlastp 3504chickpea|13v2|GR408372_P1 7078 664 88.3 globlastp 3505lotus|09v1|CB829390_P1 7079 664 85.9 globlastp 3506 prunus|10v1|BU0430837080 664 84.5 globlastp 3507 apple|11v1|CN578871_T1 7081 664 84.47glotblastn 3508 prunus_mume|13v1|BU045798_P1 7082 664 84 globlastp 3509clementine|11v1|CB610770_P1 7083 664 84 globlastp 3510euphorbia|11v1|AW944689_P1 7084 664 84 globlastp 3511oak|10v1|SRR006307S0005449_P1 7085 664 84 globlastp 3512watermelon|11v1|DV633132 7086 664 83.6 globlastp 3513grape|11v1|GSVIVT01015349001_P1 7087 664 83.5 globlastp 3514humulus|11v1|SRR098683X103251_P1 7088 664 83.5 globlastp 3515tripterygium|11v1|SRR098677X11113 7089 664 83.5 globlastp 3516beech|11v1|SRR006293.30095_P1 7090 664 83 globlastp 3517chestnut|gb170|SRR006295S0023952_P1 7091 664 83 globlastp 3518nasturtium|11v1|SRR032558.248195_P1 7092 664 83 globlastp 3519beech|11v1|SRR006293.28912_P1 7093 664 82.5 globlastp 3520cacao|10v1|CA798448_P1 7094 664 82.5 globlastp 3521cotton|11v1|DV850036_P1 7095 664 82.5 globlastp 3522cotton|11v1|DW484523_P1 7095 664 82.5 globlastp 3523gossypium_raimondii|12v1|DV850036_P1 7095 664 82.5 globlastp 3524heritiera|10v1|SRR005794S0001798_P1 7096 664 82.5 globlastp 3525sesame|12v1|SESI12V1399863 7097 664 82.5 globlastp 3526flaveria|11v1|SRR149232.13754_T1 7098 664 82.04 glotblastn 3527cassava|09v1|DV448411_P1 7099 664 82 globlastp 3528euonymus|11v1|SRR070038X352059_P1 7100 664 82 globlastp 3529poplar|10v1|BI130347 7101 664 82 globlastp 3530 poplar|13v1|BI130347_P17101 664 82 globlastp 3531 castorbean|12v1|XM_002514219_P1 7102 664 81.6globlastp 3532 olea|13v1|SRR014464X278214D1_P1 7103 664 81.6 globlastp3533 cannabis|12v1|SOLX00001727_P1 7104 664 81.6 globlastp 3534castorbean|11v1|XM_002514219 7102 664 81.6 globlastp 3535cotton|11v1|AI726553_P1 7105 664 81.6 globlastp 3536tabernaemontana|11v1|SRR098689X137108 7106 664 81.6 globlastp 3537cannabis|12v1|SOLX00054706_T1 7107 664 81.55 glotblastn 3538eucalyptus|11v2|CT982143_T1 7108 664 81.55 glotblastn 3539melon|10v1|DV633132_P1 7109 664 81.2 globlastp 3540primulua|11v1|SRR098679X10210_P1 7110 664 81.2 globlastp 3541olea|13v1|SRR014466X5637D1_P1 7111 664 81.1 globlastp 3542antirrhinum|gb166|AJ560029_P1 7112 664 81.1 globlastp 3543blueberry|12v1|CF811518_P1 7113 664 81.1 globlastp 3544kiwi|gb166|FG399558_P1 7114 664 81.1 globlastp 3545flaveria|11v1|SRR149229.174318_P1 7115 664 80.8 globlastp 3546sunflower|12v1|CD855541 7116 664 80.8 globlastp 3547sunflower|12v1|EE642451 7116 664 80.8 globlastp 3548cucumber|09v1|DV633132_P1 7117 664 80.7 globlastp 3549prunus_mume|13v1|PMBJFU1207632_P1 7118 664 80.6 globlastp 3550centaurea|11v1|EH780567_T1 7119 664 80.58 glotblastn 3551cirsium|11v1|SRR346952.1003427_T1 7119 664 80.58 glotblastn 3552cirsium|11v1|SRR346952.102086_T1 7120 664 80.58 glotblastn 3553fraxinus|11v1|SRR058827.171336_T1 7121 664 80.58 glotblastn 3554ambrosia|11v1|SRR346935.165509_P1 7122 664 80.3 globlastp 3555flaveria|11v1|SRR149229.499363_P1 7123 664 80.3 globlastp 3556centaurea|11v1|EH732220_T1 7124 664 80.1 glotblastn 3557b_juncea|12v1|E6ANDIZ01C390H_P1 7125 664 80.1 globlastp 3558b_juncea|12v1|E6ANDIZ01CPHEG_P1 7125 664 80.1 globlastp 3559b_oleracea|gb161|DY028354_P1 7125 664 80.1 globlastp 3560b_rapa|11v1|BG544180_P1 7125 664 80.1 globlastp 3561canola|11v1|CN726117_P1 7125 664 80.1 globlastp 3562canola|11v1|CN736264_P1 7125 664 80.1 globlastp 3563centaurea|gb166|EH732220 7126 664 80.1 glotblastn 3564lettuce|12v1|DW093096_T1 7127 664 80.1 glotblastn 3565monkeyflower|10v1|GR085098 7128 664 80.1 globlastp 3566monkeyflower|12v1|GR145075_P1 7128 664 80.1 globlastp 3567orbanche|10v1|SRR023189S0008854_P1 7129 664 80.1 globlastp 3568radish|gb164|EV528371 7125 664 80.1 globlastp 3569 radish|gb164|EX7502947125 664 80.1 globlastp 3570 bean|12v2|CB539867_P1 7130 665 85.8globlastp 3571 pigeonpea|11v1|SRR054580X1192_P1 7131 665 82.1 globlastp3572 soybean|12v1|GLYMA17G05000_T1 7132 666 95.81 glotblastn 3573soybean|11v1|GLYMA17G05000 7133 666 88.4 globlastp 3574bean|12v2|FE897475_P1 7134 666 88.2 globlastp 3575 bean|12v1|FE8974757134 666 88.2 globlastp 3576 lotus|09v1|AW428750_P1 7135 666 82.2globlastp 3577 pigeonpea|11v1|SRR054580X20040_P1 7136 667 89.4 globlastp3578 cowpea|12v1|FG851264_P1 7137 667 85 globlastp 3579bean|12v2|SRR001335.259534_P1 7138 667 84.3 globlastp 3580bean|12v1|SRR00334.257821 7138 667 84.3 globlastp 3581chickpea|13v2|SRR133517.17277_P1 7139 667 81.7 globlastp 3582chickpea|11v1|SRR133517.17277 7140 667 81.4 globlastp 3583bean|12v2|FE897233_P1 7141 668 89.6 globlastp 3584pigeonpea|11v1|SRR054580X108302_P1 7142 668 83.9 globlastp 3585lotus|09v1|GO023895_P1 7143 668 81.8 globlastp 3586soybean|11v1|GLYMA02G10470 7144 669 97.5 globlastp 3587soybean|12v1|GLYMA02G10470_P1 7144 669 97.5 globlastp 3588pigeonpea|11v1|SRR054580X101289_P1 7145 669 95.8 globlastp 3589bean|12v2|CA900637_P1 7146 669 94.7 globlastp 3590 bean|12v1|CA9006377146 669 94.7 globlastp 3591 chickpea|13v2|CD766047_P1 7147 669 93.3globlastp 3592 medicago|12v1|AW171668_P1 7148 669 92.8 globlastp 3593soybean|11v1|GLYMA20G23200 7149 669 88.8 globlastp 3594soybean|12v1|GLYMA20G23200_P1 7149 669 88.8 globlastp 3595lotus|09v1|CRPLJ004964_P1 7150 669 88.2 globlastp 3596pigeonpea|11v1|SRR054580X108320_P1 7151 669 88.2 globlastp 3597bean|12v2|SRR001334.240240_P1 7152 669 87.9 globlastp 3598chickpea|13v2|FE673041_P1 7153 669 85.7 globlastp 3599watermelon|11v1|AM737897 7154 669 81.65 glotblastn 3600cucumber|09v1|DV737634_T1 7155 669 81.46 glotblastn 3601thellungiella_halophilum|11v1|DN776865 7156 669 80.4 globlastp 3602soybean|11v1|GLYMA16G04520 7157 670 97.3 globlastp 3603soybean|12v1|GLYMA16G04510_P1 7157 670 97.3 globlastp 3604pigeonpea|11v1|SRR054580X109447_P1 7158 670 91.21 glotblastn 3605bean|12v2|CA910607_P1 7159 670 90.8 globlastp 3606chickpea|13v2|SRR133517.187864_P1 7160 670 84.1 globlastp 3607lotus|09v1|BW594470_P1 7161 670 82.7 globlastp 3608trigonella|11v1|SRR066194X185876 7162 670 82.37 glotblastn 3609pea|11v1|AF139187_P1 7163 670 82.3 globlastp 3610medicago|12v1|AW256421_P1 7164 670 81.4 globlastp 3611cowpea|12v1|FG818026_T1 7165 670 80.37 glotblastn 3612peanut|10v1|ES722519_P1 7166 670 80.2 globlastp 3613solanum_phureja|09v1|SPHAA824906 7167 672 99.3 globlastp 3614eggplant|10v1|FS017432_P1 7168 672 98.6 globlastp 3615tobacco|gb162|DW002909 7169 672 98.6 globlastp 3616pepper|12v1|BM064462_P1 7170 672 97.1 globlastp 3617petunia|gb171|CV292833_P1 7171 672 96.5 globlastp 3618ipomoea_nil|10v1|CJ54261_P1 7172 672 93.5 globlastp 3619nicotiana_benthamiana|12v1|DV15895 7173 672 92.8 globlastp 3620nicotiana_benthamiana|12v1|GO612443_P1 7174 672 92.8 globlastp 3621eucalyptus|11v2|CD669763_P1 7175 672 92.8 globlastp 3622papaya|gb165|EX266017_P1 7176 672 92.8 globlastp 3623tobacco|gb162|DV158950 7173 672 92.8 globlastp 3624sarracenia|11v1|SRR192669.14552 7177 672 92 globlastp 3625tabernaemontana|11v1|SRR098689X102972 7178 672 92 globlastp 3626tripterygium|11v1|SRR098677X130152 7179 672 92 globlastp 3627vinca|11v1|SRR098690X322602 7180 672 92 globlastp 3628amsonia|11v1|SRR098688X129449_P1 7181 672 91.3 globlastp 3629beech|11v1|SRR006294.27495_P1 7182 672 91.3 globlastp 3630blueberry|12v1|SRR353282X12628D1_P1 7183 672 91.3 globlastp 3631cassava|09v1|DV451280_P1 7184 672 91.3 globlastp 3632chestnut|gb170|SRR006295S0028173_P1 7182 672 91.3 globlastp 3633euonymus|11v1|SRR070038X515004_P1 7185 672 91.3 globlastp 3634grape|11v1|GSVIVT01020782001_P1 7186 672 91.3 globlastp 3635grape|11v1|VIVCRP029347_P1 7186 672 91.3 globlastp 3636oak|10v1|FP037758_P1 7182 672 91.3 globlastp 3637pigeonpea|11v1|GW359427_P1 7187 672 91.3 globlastp 3638poplar|10v1|AI161645 7188 672 91.3 globlastp 3639poplar|13v1|AI161645_P1 7188 672 91.3 globlastp 3640poplar|10v1|AI163089 7188 672 91.3 globlastp 3641poplar|13v1|AI163778_P1 7188 672 91.3 globlastp 3642sarracenia|11v1|SRR192669.105170 7189 672 91.3 glotblastn 3643soybean|11v1|GLYMA05G32260 7190 672 91.3 globlastp 3644soybean|12v1|GLYMA05G32260_P1 7190 672 91.3 globlastp 3645soybean|11v1|GLYMA16G09890 7191 672 91.3 globlastp 3646soybean|12v1|GLYMA16G09890_P1 7191 672 91.3 globlastp 3647bean|12v2|CA902328_P1 7192 672 90.6 globlastp 3648 bean|12v2|CA9023287192 672 90.6 globlastp 3649 cassava|09v1|TMPLEG659528T1_P1 7193 67290.6 globlastp 3650 castorbean|12v1|EG659528_P1 7194 672 90.6 globlastp3651 clementine|1v1|CF835589_P1 7195 672 90.6 globlastp 3651orange|11v1|CK935472_P1 7195 672 90.6 globlastp 3652coffea|10v1|DV665605_P1 7196 672 90.6 globlastp 3653cowpea|12v1|FF390716_P1 7192 672 90.6 globlastp 3654cyamopsis|10v1|EG975056_P1 7197 672 90.6 globlastp 3655liquorice|gb171|FS258113_P1 7192 672 90.6 globlastp 3656lotus|09v1|GO019310_P1 7199 672 90.6 globlastp 3657soybean|11v1|GLYMA03G22220 7199 672 90.6 globlastp 3658soybean|12v1|GLYMA03G22200_P1 7198 672 90.6 globlastp 3659trigonella|11v1|sRR066194X126359 7200 672 90.6 globlastp 3660chickpea|13v2|GR913036_P1 7200 672 89.9 globlastp 3661chickpea|13v2|SRR133517.234652_P1 7201 672 89.9 globlastp 3662bruguiera|gb166|BP950443_P1 7200 672 89.9 globlastp 3663chickpea|11v1|GR913036 7202 672 89.9 globlastp 3664cotton|11v1|AI730065_P1 7202 672 89.9 globlastp 3665cotton|11v1|BQ410573_P1 7202 672 89.9 globlastp 3666curcurbita|11v1|SRR091276X110065_P1 7203 672 89.9 globlastp 3667curcurbita|11v1|SRR091276X231682_P1 7203 672 89.9 globlastp 3668fraxinus|11v1|SRR058827.107668_P1 7204 672 89.9 globlastp 3669gossypium_raimondii|12v1|AI730065_P1 7202 672 89.9 globlastp 3670gossypium_raimondii|12v1|BQ410573_P1 7202 672 89.9 globlastp 3671heritiera|10v1|SRR005795S0002072_P1 7202 672 89.9 globlastp 3672hornbeam|12v1|SRR364455.105030_P1 7205 672 89.9 globlastp 3673medicago|12v1|AW287968_P1 7206 672 89.9 globlastp 3674momordica|10v1|SRR071315S0006698_P1 7207 672 89.9 globlastp 3675olea|11v1|SRR014463.16031 7204 672 89.9 globlastp 3676olea|13v1|SRR014463X16031D1_P1 7204 672 89.9 globlastp 3677peanut|10v1|CD038051_P1 7208 672 89.9 globlastp 3678petunia|gb171|CV298055_P1 7209 672 89.9 globlastp 3679phyla|11v2|sRR099037X109250_P1 7210 672 89.9 globlastp 3680sunflower|12v1|EE651559 7211 672 89.9 globlastp 3681walnuts|gb166|CV195160 7212 672 89.9 globlastp 3682rhizophora|10v1SRR005792S0002572 7213 672 89.86 glotblastn 3683cacao|10v1|CU480658_P1 7214 672 89.1 globlastp 3684catharanthus|11v1|EG561727_P1 7215 672 89.1 globlastp 3685cichorium|gb171|EH699665_P1 7216 672 89.1 globlastp 3686curcurbital|11v1|sRR091276X11558_P1 7217 672 89.1 globlastp 3687euonymus|11v1|SRR070038X443151_P1 7218 672 89.1 globlastp 3688euphorbia|11v1|DV116081_P1 7219 672 89.1 globlastp 3689flaveria|11v1|SRR149229.149165XX2_P1 7220 672 89.1 globlastp 3690flaveria|11v1|SRR149232.124563_P1 7220 672 89.1 globlastp 3691flaveria|11v1|SRR149232.141798_P1 7220 672 89.1 globlastp 3692guizotia|10v1|GE563099_P1 7221 672 89.1 globlastp 3693nasturtium|11v1SRR032558.175411_P1 7222 672 89.1 globlastp 3694spurge|gb161|DV116081 7219 672 89.1 globlastp 3695sunflower|12v1|AJ318322 7223 672 89.1 globlastp 3696sunflower|12v1|CF087693 7223 672 89.1 globlastp 3697watermelon|11v1|AM714594 7224 672 89.1 globlastp 3698aquilegia|10v2|JGIAC003626_P1 7225 672 88.5 globlastp 3699ambrosia|11v1|SRR346943.116345_P1 7226 672 88.4 globlastp 3700ambrosia|11v1|SRR346943.135917_P1 7226 672 88.4 globlastp 3701blueberry|12v1|CV190540_P1 7227 672 88.4 globlastp 3702blueberry|12v1|SRR353282X5502D1_P1 7227 672 88.4 globlastp 3703cucumber|09v1|AM714594_P1 7228 672 88.4 globlastp 3704lettuce|12v1|DW050615_P1 7229 672 88.4 globlastp 3705melon|10v1|AM714594_P1 7228 672 88.4 globlastp 3706nuphar|gb166|CK760631_P1 7230 672 88.4 globlastp 3707sunflower|12v1|CD847025 7231 672 88.4 globlastp 3708sunflower|12v1|DY935727 7232 672 88.4 globlastp 3709trapopogon|10v1|SRR020205S0012851 7233 672 88.4 globlastp 3710watermelon|11v1|VMEL08104207201030 7234 672 88.4 globlastp 3711valeriana|11v1|SRR099039X129790 7235 672 87.9 globlastp 3712flax|11v1|JG083843_P1 7236 672 87.7 globlastp 3713antirrhinum|gb166|AJ788528_T1 7237 672 87.68 glotblastn 3714amborella|12v3|SRR038635.101488_P1 7238 672 87.1 globlastp 3715dandelion|10v1|DR402437_P1 7239 672 87.1 globlastp 3716apple|11v1|CN445348_P1 7240 672 87 globlastp 3717artemisal|10v1|EF549581_P1 7241 672 87 globlastp 3718cleome_gynandra|10v1|SRR015532S0004149_P1 7242 672 87 globlastp 3719cleome_spinosa|10v1|SRR015531S0006728_P1 7243 672 87 globlastp 3720euphorbia|11v1|SRR098678X163100_P1 7244 672 87 globlastp 3721flax|11v1|JG023417_P1 7245 672 87 globlastp 3722oil_palm|11v1|EL683706_P1 7246 672 87 globlastp 3723prunus|10v1|BU043746 7247 672 87 globlastp 3724 rose|12v1|BI978180 7248672 87 globlastp 3725 rose|12v1|EC588741 7248 672 87 globlastp 3726chelidonium|11v1|SRR084752X170599_T1 7249 672 86.96 glotblastn 3727primula|11v1|SRR098679X102937_T1 7250 672 86.96 glotblastn 3728banana|12v1|BBS2839T3_P1 7251 672 86.2 globlastp 3729eschscholzia|11v1|CK748022_P1 7252 672 86.2 globlastp 3730monkeyflower|10v1|CV518516 7253 672 86.2 globlastp 3731monkeyflower|12v1|CV518516_P1 7253 672 86.2 globlastp 3732centaurea|11v1|EH738332_P1 7254 672 85.7 globlastp 3733centaurea|11v1|EH752889_P1 7254 672 85.7 globlastp 3734centaurea|gb166|EH738332 7254 672 85.7 globlastp 3735cirsium|11v1|sRR346952.1024531_P1 7254 672 85.7 globlastp 3736olea|13v1|SRR014463X44251D1_T1 7255 672 85.61 glotblastn 3737aristolochia|10v1|FD750215_P1 7256 672 85.5 globlastp 3738bupleurum|11v1|sRR301254.1230037_P1 7257 672 85.5 globlastp 3739cucumber|09v1|BG1454H0182627_P1 7258 672 85.5 globlastp 3740fogopyrum|11v1|SRR063689X101809_P1 7259 672 85.5 globlastp 3741fogopyrum|11v1|SRR063689X103813_P1 7259 672 85.5 globlastp 3742utricularia|11v1|SRR0494438.101455 7260 672 85.5 globlastp 3743senecio|gb170|DY659690 7261 672 85.1 globlastp 3744centaurea|11v1|EH780576_P1 7262 672 85 globlastp 3745arabidopsis_lyrata|09v1|JGIAL020672_P1 7264 672 84.8 globlastp 3746arabidopsis|10v1|AT5G09920_P1 7264 672 84.8 globlastp 3747fagopyrum|11v1|SRR063703X131004_P1 7265 672 84.8 globlastp 3748sesame|12v1|SESI12V1356715 7266 672 84.8 globlastp 3749euphorbia|11v1|BP960506_P1 7267 672 84.1 globlastp 3750b_rapa|11v1|CN730236_P1 7268 672 84.1 globlastp 3751b_rapa|11v1|H07557_P1 7268 672 84.1 globlastp 3752canola|11v1|CN730236_P1 7268 672 84.1 globlastp 3753canola|11v1|EE459982_P1 7268 672 84.1 globlastp 3754canola|11v1|EV041760_P1 7268 672 84.1 globlastp 3755canola|11v1|H07557_P1 7268 672 84.1 globlastp 3756humulus|11v1|SRR098683X106032_P1 7269 672 84.1 globlastp 3757radish|gb164|EV544637 7268 672 84.1 globlastp 3758thellungiella_parvulum|11v1|DN774731 7270 672 84.1 globlastp 3759cynara|gb167|GE586010_P1 7271 672 83.6 globlastp 3760radish|gb164|EW716919 7272 672 83.5 globlastp 3761 radish|gb164|EX7639627272 672 83.5 globlastp 3762 curcuma|10v1|DY383869_T1 7273 672 83.33glotblastn 3763 aristolochia|10v1|SRR03982S0316312_P1 7274 672 83.3globlastp 3764 b_juncea|12v1|E6ANDIZ01B54CY_P1 7275 672 83.3 globlastp3765 b_juncea|12v1|E6ANDIZ01CNNVD_P1 7276 672 83.3 globlastp 3766b_oleracea|gb161|AM057678_P1 7276 672 83.3 globlastp 3767canola|11v1|CN828755_P1 7277 672 83.3 globlastp 3768phalaenopsis|11v1|sRR125771.1055688_P1 7278 672 83.3 globlastp 3769salvia|10v1|CV165847 7279 672 83.3 globlastp 3770strawberry|11v1|CO379850 7280 672 83.3 globlastp 3771safflower|gb162|EL387455 7281 672 82.9 globlastp 3772canola|11v1|EG019489_P1 7282 672 82.7 globlastp 3773b_rapa|11v1|CD811778_P1 7283 672 82.1 globlastp 3774canola|11v1|ES959288_P1 7283 672 82.1 globlastp 3775canola|11v1|FG570031_P1 7284 672 81.9 globlastp 3776fraxinus|11v1|SRR058827.104121_P1 7285 672 81.9 globlastp 3777thellungiella_halophilum|11v1|DN774731 7286 672 81.9 globlastp 3778phyla|11v2|SRR099035X34009_T1 7287 672 81.88 glotblastn 3779fagopyrum|11v1|SRR063689X155135_T1 — 672 81.88 glotblastn 3780cirsium|11v1|SRR346952.454216_P1 7288 672 81.4 globlastp 3781catharanthus|11v1|SRR098691X345081_T1 7289 672 80.43 glotblastn 3782cycas|gb166|EX920938_T1 7290 672 80.43 glotblastn 3783nicotiana_benthamiana|12v1|AJ718885_P1 7291 672 80.4 globlastp 3784potato|10v1|BG350925_P1 7292 673 97.9 globlastp 3785solanum_phureja|09v1|SPHBG630567 7292 673 97.9 globlastp 3786nicotiana_benthamiana|12v1|AF153277_P1 7293 673 95.8 globlastp 3787nicotiana_benthamiana|12v1|EH367819_P1 7293 673 95.8 globlastp 3788tobacco|gb162|AF153277 7293 673 95.8 globlastp 3789pepper|12v1|BM064474_P1 7294 673 95.1 globlastp 3790eggplant|10v1|FS029867_P1 7295 673 93.8 globlastp 3791tobacco|gb162|AM784837 7296 673 88.9 globlastp 3792monkeyflower|10v1|DV210285 7297 673 87.5 globlastp 3793monkeyflower|12v1|DV210285_P1 7297 673 87.5 globlastp 3794orobanche|10v1|SRR023189S0013058_P1 7298 673 87.5 globlastp 3795petunia|gb171|CV295124_P1 7299 673 87.5 globlastp 3796orobanche|10v1|SRR023189S0021390_P1 7300 673 86.8 globlastp 3797triphysaria|10v1|BM357267 7301 673 86.8 globlastp 3798olea|13v1|SRR014464X38751D1_P1 7302 673 86.1 globlastp 3799catharanthus|11v1|SRR098691X105656_P1 7303 673 86.1 globlastp 3800fraxinus|11v1|SRR058827.108943_P1 7304 673 86.1 globlastp 3801fraxinus|11v1|SRR058827.112759XX1_P1 7305 673 86.1 globlastp 3802olea|11v1|SRR014463.10448 7306 673 86.1 globlastp 3803olea|13v1|SRR014463X10448D1_P1 7307 673 86.1 globlastp 3804phyla|11v2|SRR099035X151618_P1 7308 673 86.1 globlastp 3805sesame|12v1|JK050928 7309 673 86.1 globlastp 3806phyla|11v2|SRR099037X276914_T1 7310 673 85.42 globlastp 3807sesame|12v1|SESI12V1356115 7311 673 85.4 globlastp 3808triphysaria|10v1|EX997442 7312 673 85.4 globlastp 3809blueberry|12v1|CV191473_P1 7313 673 84.8 globlastp 3810blueberry|12v1|SRR353282X102149D1_P1 7313 673 84.8 globlastp 3811blueberry|12v1|SRR353282X17149D1_P1 7314 673 84.8 globlastp 3812blueberry|12v1|SRR353282X66118D1_P1 7314 673 84.8 globlastp 3813platanus|11v1|SRR096786X104548_P1 7315 673 84.8 globlastp 3814platanus|11v1|SRR096786X127843_P1 7316 673 84.8 globlastp 3815nicotiana_benthamiana|12v1|FG637079_T1 7317 673 84.72 glotblastn 3816cucurbita|11v1|sRR091276X10598_P1 7318 673 84.2 globlastp 3817phalaenopsis|11v1|CB034306_P1 7319 673 84.2 globlastp 3818sarracenia|11v1|SRR192669.141782 7320 673 84.2 globlastp 3819watermelon|11v1|VMEL00232223443500 7321 673 84.2 globlastp 3820fraxinus|11v1|SRR058827.115881_T1 7322 673 84.03 glotblastn 3821coffea|10v1|DV672264_P1 7323 673 84 globlastp 3822phyla|11v2|SRR099037X112957_P1 7324 673 84 globlastp 3823vinca|11v1|sRR098690X103362 7325 673 84 globlastp 3824vinca|11v1|sRR098690X197340 7326 673 84 globlastp 3825melon|10v1|DV634597_P1 7327 673 83.6 globlastp 3826momordica|10v1|SRR071315S0002594_P1 7328 673 83.6 globlastp 3827pigeonpea|11v1|SRR054580X113200_P1 7329 673 83.6 globlastp 3828ambrosia|11v1|SRR346935.1550_P1 7330 673 83.4 globlastp 3829blueberry|12v1|SRR353282X101052D1_P1 7331 673 83.4 globlastp 3830chelidonium|11v1|SRR084752X106486_P1 7332 673 83.4 globlastp 3831onion|12v1|SRR073446X111946D1_P1 7333 673 83.4 globlastp 3832onion|12v1|SRR073446X346775D1_P1 7333 673 83.4 globlastp 3833parthenium|10v1|GW777925_P1 7330 673 83.4 globlastp 3834apple|11v1|CN994802_P1 7334 673 83.1 globlastp 3835oil_palm|11v1|EY404310_P1 7335 673 83 globlastp 3836bruguiera|gb166|BP942698_P1 7336 673 82.9 globlastp 3837euphorbia|11v1|BP955493_P1 7337 673 82.9 globlastp 3838flaveria|11v1|SRR149229.101882_P1 7338 673 82.9 globlastp 3839flaveria|11v1|SRR149232.120155_P1 7339 673 82.9 globlastp 3840oil_palm|11v1|sRR190698.121788_P1 7340 673 82.9 globlastp 3841sunflower|12v1|CD847151 7341 673 82.9 globlastp 3842sunflower|12v1|CF077747 7341 673 82.9 globlastp 3843watermelon|11v1|DV634597 7342 673 82.9 globlastp 3844kiwi|gb166|FG402684_P1 7343 673 82.8 globlastp 3845sunflower|12v1|AJ828557 7344 673 82.8 globlastp 3846sunflower|12v1|EE650599 7345 673 82.8 globlastp 3847fraxinus|11v1|SRR058827.129416_P1 7346 673 82.6 globlastp 3848lettuce|12v1|DW045150_P1 7347 673 82.3 globlastp 3849artemisia|10v1|EY046558_P1 7348 673 82.2 globlastp 3850banana|12v1|FF560328_P1 7349 673 82.2 globlastp 3851cassava|09v1|DV445147_P1 7350 673 82.2 globlastp 3852cucurbita|11v1|SRR091276X115032_P1 7351 673 82.2 globlastp 3853euphorbia|11v1|DV113111_P1 7352 673 82.2 globlastp 3853spurge|gb161|DV113111 7352 673 82.2 globlastp 3854 poplar|10v1|AI1629877353 673 82.2 globlastp 3855 poplar|13v1|AI162987_P1 7353 673 82.2globlastp 3856 sunflower|12v1|EE653266 7354 673 82.2 globlastp 3857sarracenia|11v1|SRR192669.100134 7355 673 82.19 glotblastn 3858peach|gb157.2|DY639276_P1 7356 673 82.1 globlastp 3859prumus|10v1|CN994802 7356 673 82.1 globlastp 3860strawberry|11v1|EX658203 7357 673 82.1 globlastp 3861amsonia|11v1|sRR098688X106478_P1 7358 673 81.9 globlastp 3862antirrhinum|gb166|AJ790220_P1 7359 673 81.9 globlastp 3863ipomoea_nil|10v1|CJ745389_P1 7360 673 81.9 globlastp 3864cichorium|gb171|EH701034_P1 7361 673 81.6 globlastp 3865guizotia|10v1|GE561736_T1 7362 673 81.51 glotblastn 3866ambrosia|11v1|SRR346943.108668_P1 7363 673 81.5 globlastp 3867ambrosia|11v1|SRR346947.123392_P1 7364 673 81.5 globlastp 3868banana|12v1|FF558025_P1 7365 673 81.5 globlastp 3869cucumber|09v1|DV634597_P1 7366 673 81.5 globlastp 3870flaveria|11v1|SRR149229.46654_P1 7367 673 81.5 globlastp 3871aristolochia|10v1|SRR039082S0013912_P1 7368 673 81.4 globlastp 3872eucalyptus|11v2|CD669833_P1 7369 673 81.4 globlastp 3873grape|11v1|GSVIVT01010613001_P1 7370 673 81.4 globlastp 3874oak|10v1|FP027819_P1 7371 673 81.4 globlastp 3875onion|12v1|SRR073446X118851D1_P1 7372 673 81.4 globlastp 3876rose|12v1|SRR397984.109724 7373 673 81.4 globlastp 3877ginger|gb164|DY349157_T1 7374 673 81.38 glotblastn 3878gergera|09v|AJ754249_P1 7375 673 81.2 globlastp 3879primulal|11v1|SRR098679X104604_P1 7376 673 81.2 globlastp 3880cassava|09v1|CK645566_P1 7377 673 80.8 globlastp 3881euonymus|11v1|SRR070038X13696_P1 7378 673 80.8 globlastp 3882euonymus|11v1|SRR070038X141580_P1 7379 673 80.8 globlastp 3883flaveria|11v1|SRR149241.115333_P1 7380 673 80.8 globlastp 3884pigeonpea|11v1|SRR054580X106914_P1 7381 673 80.8 globlastp 3885poplar|10v1|CK117372 7382 673 80.8 globlastp 3886poplar|13v1|CK117372_P1 7382 673 80.8 globlastp 3887tripterygium|11v1|sRR098677X118682 7383 673 80.8 globlastp 3888cirsium|11v1|SRR346952.116865_P1 7384 673 80.7 globlastp 3889eschscholzia|11v1|SRR014116.117187_P1 7385 673 80.7 globlastp 3890humulus|11v1|ES653528_P1 7386 673 80.7 globlastp 3891onion|12v1|SRR073446X11066D1_P1 7387 673 80.7 globlastp 3892beech|11v1|SRR006293.23858_T1 7388 673 80.69 glotblastn 3893cirsium|11v1|SRR346952.1090555_P1 7389 673 80.3 globlastp 3894cynara|gb167|GE586553_P1 7390 673 80.3 globlastp 3895cynara|gb167|GE591412_P1 7391 673 80.3 globlastp 3896medicago|12v1|BE315728_P1 7392 673 80.3 globlastp 3897trigonella|11v1|SRR066194X152657 7393 673 80.3 globlastp 3898avocado|10v1|FD508333_T1 7394 673 80.27 glotblastn 3899clementine|11v1|CF417220_P1 7395 673 80.1 globlastp 3900euonymus|11v1|SRR070038X134316_P1 7396 673 80.1 globlastp 3901euonymus|11v1|SRR070038X175696_P1 7397 673 80.1 globlastp 3902foxtail_millet|11v3|PHY7SI037339M_P1 7398 673 80.1 globlastp 3903hornbeam|12v1|SRR364455.10539_P1 7399 673 80.1 globlastp 3904orange|11v1|CF417220_P1 7395 673 80.1 globlastp 3905 rice|11v1|BI8000037400 673 80.1 globlastp 3906 walnuts|gb166|EL892024 7401 673 80.1globlastp 3907 prunus_mume|13v1|DY639276_P1 7402 673 80 globlastp 3908acacia|10v1|GR481215_P1 7403 673 80 globlastp 3909amorphophallus|11v2|SRR089351X21955_P1 7404 673 80 globlastp 3910aquilegia|10v2|JGIAC024478_T1 7405 673 80 glotblastn 3911chestnut|gb170|SRR006295S0000818_P1 7406 673 80 globlastp 3912eschscolzia|11v1|SRR014116.113832_P1 7407 673 80 globlastp 3913nasturtium|11v1|GH169570_P1 7408 673 80 globlastp 3914oak|10v1|DB997979_P1 7409 673 80 globlastp 3915solanum_phureja|09v1|SPHAW034456 7410 674 96.6 globlastp 3916potato|10v1|CK245349_P1 7411 674 94.9 globlastp 3917eggplant|10v1|FS007122_P1 7412 674 88.7 globlastp 3918nicotiana_benthamiana|12v1|BP745922_P1 7413 674 88.1 globlastp 3919nicotiana_benthamiana|12v1|EH369150_P1 7414 674 87.6 globlastp 3920pepper|12v1|GD060497_T1 7415 674 87.01 glotblastn 3921potato|10v1|BQ514168_P1 7416 675 98.1 globlastp 3922solanum_phureja|09v1|SPHAW617278 7417 675 97.2 globlastp 3923pepper|12v1|GD080968_T1 7418 675 89.3 globlastp 3924nicotiana_benthamiana|12v1|EB429811_P1 7419 675 87.1 globlastp 3925tobacco|gb162|EB429811 7420 675 85.19 glotblastn 3926potato|10v1|CV491855_P1 7421 676 92.5 globlastp 3927solanum_phureja|09v1|SPHBG136313 7422 676 92.5 globlastp 3928eggplant|10v1|FS056643_P1 7423 676 90 globlastp 3929pepper|12v1|GD064989_P1 7424 676 90 globlastp 3930tobacco|gb162|DW004195 7425 676 89.6 globlastp 3931nicotiana_benthamiana|12v1|FG642673_P1 7426 676 89.1 globlastp 3932nicotiana_benthamiana|12v1|DV999162_P1 7427 676 88.1 globlastp 3933potato|10v1|BG596489_P1 7428 677 98.8 globlastp 3934solanum_phureja|09v1|SPHBG734868 7429 677 98.8 globlastp 3935eggplant|10v1|FS016659_P1 7430 677 96.4 globlastp 3936tobacco|gb162|CV020268 7431 677 96.4 globlastp 3937nicotiana_benthamiana|12v1|CK990177_P1 7432 677 95.2 globlastp 3938pepper|12v1|CA515174_P1 7433 677 94.1 globlastp 3939petunia|gb171|CV299571_P1 7434 677 94 globlastp 3940coffea|10v1|DV692487_P1 7435 677 89.4 globlastp 3941olea|13v1|SRR014464X31367D1_P1 7436 677 89.3 globlastp 3942ipomoea_batatas|10v1|BU691677_P1 7437 677 89.3 globlastp 3943fraxinus|11v1|SRR058827.107993_P1 7438 677 88.1 globlastp 3944fraxinus|11v1|SRR058827.190612_P1 7438 677 88.1 globlastp 3945ipomoea_nil|10v1|BJ553531_P1 7439 677 88.1 globlastp 3946olea|11v1|SRR014463.11310 7438 677 88.1 globlastp 3947olea|13v1|SRR014463X11310D1_P1 7438 677 88.1 globlastp 3948lettuce|12v1|DW066365_P1 7440 677 87.1 globlastp 3949amsonia|11v1|SRR098688X117039_P1 7441 677 86.9 globlastp 3950catharanthus|11v1|SRR098691X137558_P1 7442 677 86.9 globlastp 3951centaurea|gb166|EH711371 7443 677 86.9 globlastp 3952centaurea|11v1|EH711371_T1 7444 677 86.9 glotblastn 3953centaurea|gb166|EH740490 7443 677 86.9 globlastp 3954cirsium|11v1|SRR346952.1000422_T1 7445 677 86.9 glotblastn 3955cirsium|11v1|SRR346952.106666_P1 7443 677 86.9 globlastp 3956safflower|gb162|EL407633 7446 677 86.9 globlastp 3957sunflower|12v1|CD850724 7443 677 86.9 globlastp 3958tabernaemontana|11v1|SRR098689X105890 7447 677 86.9 globlastp 3959sunflower|12v1|CD849196 7448 677 86 globlastp 3960dandelion|10v1|DR399089_P1 7449 677 85.9 globlastp 3961artemisia|10v1|GW329794_T1 7450 677 85.88 glotblastn 3962fraxinus|11v1|SRR058827.105367_T1 7451 677 85.71 glotblastn 3963ipomoea_batatas|10v1|DV036634_T1 7452 677 85.71 glotblastn 3964arabidopsis_lyrata|09v1|JGIAL016191_P1 7453 677 85.7 globlastp 3965aristolochia|10v1|SRR03983S1062197_P1 7454 677 85.7 globlastp 3966flaveria|11v1|SRR149244.111421_P1 7455 677 85.7 globlastp 3967guizotia|10v1|GE571049_P1 7456 677 85.7 globlastp 3968phyla|11v2|SRR099035X10210_P1 7457 677 85.7 globlastp 3969phyla|11v2|SRR099035X107040_P1 7458 677 85.7 globlastp 3970platanus|11v1|SRR096786X107756_P1 7459 677 84.7 globlastp 3971platanus|11v1|SRR096786X116200_P1 7460 677 84.7 globlastp 3972b_oleracea|gb161|AM394278_T1 7461 677 84.52 glotblastn 3973cacao|10v1|CA794604_T1 7462 677 84.52 glotblastn 3974ambrosia|11v1|SRR346943.10506_P1 7463 677 84.5 globlastp 3975b_juncea|12v1|E6ANDIZ01A7LR6_P1 7464 677 84.5 globlastp 3976blueberry|12v1|SRR353282X27890D1_P1 7465 677 84.5 globlastp 3977flaveria|11v1|SRR149299.103468_P1 7466 677 84.5 globlastp 3978monkeyflower|10v1|GO964950 7467 677 84.5 globlastp 3979monkeyflower|12v1|GO997762_P1 7467 677 84.5 globlastp 3980soybean|11v1|GLYMA03G37250 7468 677 84.5 globlastp 3981soybean|12v1|GLYMA03G37250_P1 7468 677 84.5 globlastp 3982thellungiella_halophilum|11v1|EHJGI009624 7469 677 84.5 globlastp 3983thellungiella_parvulu|11v1|EPCRP016283 7470 677 84.5 globlastp 3984beech|11v1|SRR364434.196077_P1 7471 677 83.5 globlastp 3985chickpea|11v1|SRR133517.102541 7472 677 83.5 globlastp 3986chickpea|11v1|SRR133517.102541_P1 7472 677 83.5 globlastp 3987eucalyptus|11v2|SRR001659X130769_P1 7473 677 83.5 globlastp 3988teal|10v1|EU375562 7474 677 83.5 globlastp 3989castorbean|12v1|EE256065_T1 7475 677 83.33 glotblastn 3990antirrhinum|gb166|AJ790795_P1 7476 677 83.3 globlastp 3991arabidopsis_lyrata|09v1|TMPLAT3G62790T1_P1 7477 677 83.3 globlastp 3992arabidopsis|10v1|AT2G47690_P1 7478 677 83.3 globlastp 3993arabidopsis|10v1|AT3G62790_P1 7477 677 83.3 globlastp 3994b_oleracea|gb161|EE534469_P1 7479 677 83.3 globlastp 3995chestnut|gb170|SRR006295S0013415_P1 7480 677 83.3 globlastp 3996hornbean|12v1|SRR364455.108791_P1 7481 677 83.3 globlastp 3997flaveria|11v1|SRR149229.103468_P1 7466 677 84.5 globlastp 3998salvia|10v1|SRR014553S0001603 7483 677 84.5 globlastp 3999sesame|12v1|SESI12V1183062 7484 677 84.5 globlastp 4000thellungiella_halophilum|11v1|BY806974 7485 677 84.5 globlastp 4001ginseng|10v1|GR872098_P1 7486 677 82.8 globlastp 4002poplar|10v1|BU814197 7487 677 82.6 globlastp 4003poplar|13v1|BU814197_P1 7487 677 82.6 globlastp 4004castorbean|12v1|SRR020785.12360_P1 7488 677 82.4 globlastp 4005oil_palm|11v1|EL687328_P1 7489 677 82.4 globlastp 4006sarracenia|11v1|SRR192669.113844 7490 677 82.4 globlastp 4007bean|12v2|CA899125_P1 7491 677 82.1 globlastp 4008aquilegia|10v2|JGIAC017903_P1 7492 677 82.1 globlastp 4009arabidopsis_lyrata|09v1|JGIAL019629_P1 7493 677 82.1 globlastp 4010b_rapa|11v1|DC8134002_P1 7494 677 82.1 globlastp 4011 bean|12v1|CA8991257491 677 82.1 globlastp 4012 cleome_gynandra|10v1|SRR015532S0037183_P17495 677 82.1 globlastp 4013 cleome_spinosa|10v1|GR933599_P1 7496 67782.1 globlastp 4014 cowpea|12v1|FF391544_P1 7497 677 82.1 globlastp 4015epimedium|11v1|SRR013502.18250_P1 7498 677 82.1 globlastp 4016euonymus|11v1|sRR070038X129578_P1 7499 677 82.1 globlastp 4017flaveria|11v1|SRR149241.351805_P1 7500 677 82.1 globlastp 4018liquorice|gb171|FS244458_P1 7501 677 82.1 globlastp 4019medicago|12v1|AL380702_P1 7502 677 82.1 globlastp 4020monkeyflower|10v1|DV212834 7503 677 82.1 globlastp 4021monkeyflower|12v1|DV212834_P1 7503 677 82.1 globlastp 4022oak|10v1|DN950929_P1 7504 677 82.1 globlastp 4023primula|11v1|SRR098681X50499_P1 7505 677 82.1 globlastp 4024radish|gb164|EV525126 7506 677 82.1 globlastp 4025soybean|11v1|GLYMA19G39860 7507 677 82.1 globlastp 4026soybean|12v1|GLYMA19G39860_P1 7507 677 82.1 globlastp 4027thellungiella_parvulum|11v1|BY806974 7494 677 82.1 globlastp 4028triphysaria|10v1|EY139435 7508 677 82.1 globlastp 4029triphysaria|10v1|SRR023500S0005824 7509 677 82.1 globlastp 4030humulus|11v1|ES653674_P1 7510 677 81.6 globlastp 4031utricularia|11v1|sRR094438.10063 7511 677 81.4 globlastp 4032amorphophallus|11v2|SRR089351X126092_P1 7512 677 81.2 globlastp 4033cassava|09v1|JGICASSAVA1175VALIDM1_P1 7513 677 81.2 globlastp 4034cotton|11v1|AI726094_P1 7514 677 81.2 globlastp 4035gossypium_raimondii|12v1|AI726094_P1 7514 677 81.2 globlastp 4036heritiera|10v1|SRR005795S0009085_P1 7515 677 81.2 globlastp 4037pigeonpea|11v1|GW355058_P1 7516 677 81.2 globlastp 4038rose|12v1|EC587736 7517 677 81.2 globlastp 4039fraximus|11v1|sRR058827.108012_T1 7518 677 81.18 glotblastn 4040acacia|10v1|GR482262_P1 7519 677 81 globlastp 4041b_juncea|12v1|E6ANDIZ01C6DZO_P1 7520 677 81 globlastp 4042canola|11v1|CN732165_P1 7521 677 81 globlastp 4043lotus|09v1|BF177508_P1 7522 677 81 globlastp 4044poppy|11v1|SRR030259.116296_P1 7523 677 81 globlastp 4045radish|gb164|EV538661 7524 677 81 globlastp 4046 radish|gb164|EV5436477525 677 81 globlastp 4047 radish|gb164|EW723879 7526 677 81 globlastp4048 trigonella|11v1|SRR066194X114302 7527 677 81 globlastp 4049b_rapa|11v1|CD816662_T1 7528 677 80.95 glotblastn 4050b_rapa|11v1|CD817758_T1 7529 677 80.95 glotblastn 4051canola|11v1|DW999649XX2_T1 7530 677 80.95 glotblastn 4052cotton|11v1|BF274690_T1 7531 677 80.95 glotblastn 4053gossypium_raimondii|12v1|BF274690_T1 7532 677 80.95 glotblastn 4054grape|11v1|GSVIVT01035146001_P1 7533 677 80.5 globlastp 4055liquorice|gb171|FS239994_P1 7534 677 80.5 globlastp 4056apple|11v1|CN491129_P1 7535 677 80.2 globlastp 4057peach|gb157.2|AJ827105_T1 7536 677 80 glotblastn 4058prunus_mume|13v1|SRR345674.52578_T1 7536 677 80 glotblastn 4059b_junceal|12v1|E6ANDIZ01B371N_P1 7537 677 80 globlastp 4060cassava|09v1|DB924993_P1 7538 677 80 globlastp 4061chelidonium|11v1|SRR084752X13675_P1 7539 677 80 globlastp 4062cotton|11v1|BE053626_P1 7540 677 80 globlastp 4063cotton|11v1|SRR032367.1014115_P1 7540 677 80 globlastp 4064gossypium_raimondii|12v1|BE053626_P1 7540 677 80 globlastp 4065prunus|10v1|CN491129 7536 677 80 glotblastn 4066strawberry|11v1|DV439027 7541 677 80 globlastp 4067 wheat|12v3|BE4150817542 678 85.41 glotblastn 4068 rye|12v1|BE636808 7543 678 85.3 globlastp4069 rye|12v1|DRR001012.266258 7544 678 85.3 globlastp 4070rye|12v1|DRR001012.1483 7545 678 81.59 glotblastn 4071barley|12v1|BE420812_P1 7546 679 98.5 globlastp 4072barley|12v1|BI950339_P1 7546 679 98.5 globlastp 4073leymus|gb166|CD809189_P1 7546 679 98.5 globlastp 4074 rye|12v1|BE6368687547 679 98.5 globlastp 4075 rye|12v1|DRR001012.100149 7547 679 98.5globlastp 4076 rye|12v1|DRR001012.101034 7547 679 98.5 globlastp 4077rye|12v1|DRR001012.107227 7547 679 98.5 globlastp 4078rye|12v1|DRR001012.110963 7547 679 98.5 globlastp 4079rye|12v1|DRR001012.149209 7547 679 98.5 globlastp 4080rye|12v1|DRR001012.155634 7547 679 98.5 globlastp 4081rye|12v1|DRR001012.173348 7547 679 98.5 globlastp 4082wheat|12v3|BE213361 7547 679 98.5 globlastp 4083 wheat|12v3|BE2135417547 679 98.5 globlastp 4084 wheat|12v3|BE217061 7547 679 98.5 globlastp4085 wheat|12v3|BE412383 7547 679 98.5 globlastp 4086wheat|12v3|BE417966 7547 679 98.5 globlastp 4087 wheat|12v3|BE4183257547 679 98.5 globlastp 4088 wheat|12v3|BE425441 7547 679 98.5 globlastp4089 wheat|12v3|BE604046 7548 679 98.5 globlastp 4090wheat|12v3|BF478899 7547 679 98.5 globlastp 4091leymus|gb166|EG391011_P1 7549 679 98.1 globlastp 4092lolium|10v1|AU246390_P1 7550 679 98.1 globlastp 4093oat|11v1|CN818158_P1 7551 679 98.1 globlastp 4094oat|11v1|SRR020741.132872_P1 7551 679 98.1 globlastp 4095rye|12v1|DRR001012.882529 7552 679 98.1 globlastp 4096wheat|12v3|BE213314 7553 679 98.1 globlastp 4097 wheat|12v3|BG3136487554 679 97.72 glotblastn 4098 oat|11v1|CN818384_P1 7555 679 97.3globlastp 4099 rye|12v1|DRR001012.371748 7556 679 96.6 globlastp 4100wheat|12v3|BE425237 7557 679 96.6 globlastp 4101rye|12v1|DRR001012.338927 7558 679 94.72 glotblastn 4102rice|11v1|AF022739 7559 679 94.7 globlastp 4103switchgrass|12v1|SRR187768.89507_P1 7560 679 91.7 globlastp 4104maize|10v1|MZEORFI_P1 7561 679 91.7 globlastp 4105millet|10v1|EB410931_P1 7562 679 91.7 globlastp 4106switchgrass|12v1|DN143440_P1 7560 679 91.7 globlastp 4107switchgrass|gb167|DN143440 7560 679 91.7 globlastp 4108foxtail_millet|11v3|PHY7SI037076M_P1 7563 679 91.3 globlastp 4109sorghum|12v1|AW283115 7564 679 91.3 globlastp 4110sorghum|12v1|SB01G015400 7564 679 91.3 globlastp 4111switchgrass|12v1|DN144083_T1 7565 679 90.57 glotblastn 4112switchgrass|12v1|DN147434_T1 7566 679 90.57 glotblastn 4113cynodon|10v1|ES292265_P1 7567 679 90.5 globlastp 4114lovegrass|gb167|DN480829_P1 7568 679 89 globlastp 4115oil_palm|11v1|SRR190698.280201_P1 7569 679 89 globlastp 4116antirrhinum|gb166|AJ568367_P1 7570 679 88.8 globlastp 4117aristolochia|10v1|SRR039085S0197472_P1 7571 679 88.7 globlastp 4118curcurbital|11v1|SRR091276X104071_P1 7572 679 88.7 globlastp 4119kiwi|gb166|FG397198_P1 7573 679 88.3 globlastp 4120liriodendron|gb166|CK767175_P1 7574 679 88.3 globlastp 4121cucumber|09v1|AA660116_P1 7575 679 88 globlastp 4122flaveria|11v1|SRR149232.119275_P1 7576 679 88 globlastp 4123poplar|10v1|AI164040 7577 679 88 globlastp 4124 teal|10v1|CV013768 7578679 88 globlastp 4125 wheat|12v3|CJ716221 7579 679 87.8 globlastp 4126poplar|13v1|AI162183_P1 7580 679 87.6 globlastp 4127ambrosia|11v1|SRR346943.12315_P1 7581 679 87.6 globlastp 4128cannabis|12v1|EW700699_P1 7582 679 87.6 globlastp 4129cannabis|12v1|GR221144_P1 7582 679 87.6 globlastp 4130chestnut|gb170|AF417304_P1 7583 679 87.6 globlastp 4131eschschlzia|11v1|CK764821_P1 7584 679 87.6 globlastp 4132flaveria|11v1|SRR149229.103066_P1 7585 679 87.6 globlastp 4133flaveria|11v1|SRR149229.133211_P1 7585 679 87.6 globlastp 4134flaveria|11v1|SRR149229.26461XX1_P1 7585 679 87.6 globlastp 4135flaveria|11v1|SRR149232.115148_P1 7585 679 87.6 globlastp 4136flaveria|11v1|SRR149232.145844_P1 7586 679 87.6 globlastp 4137flaveria|11v1|SRR149232.190929_P1 7585 679 87.6 globlastp 4138flaveria|11v1|SRR149241.10181_P1 7585 679 87.6 globlastp 4139grape|11v1|GSVOVT01030053001_P1 7587 679 87.6 globlastp 4140jatropha|09v1|GH295683_P1 7588 679 87.6 globlastp 4141melon|10v1|CF674904_P1 7589 679 87.6 globlastp 4142 oak|10v1|CU657887_P17583 679 87.6 globlastp 4143 oak|10v1|CU657896_P1 7583 679 87.6globlastp 4144 oak|10v1|DB996682_P1 7583 679 87.6 globlastp 4145oak|10v1|DB998065_P1 7583 679 87.6 globlastp 4146 oak|10v1|FN718861_P17583 679 87.6 globlastp 4147 oak|10v1|FP045120_P1 7583 679 87.6globlastp 4148 oak|10v1|SRR006307S0007311_P1 7583 679 87.6 globlastp4149 oak|10v1|SRR039734S0015335_P1 7583 679 87.6 globlastp 4150oak|10v1|SRR039734S0080903_P1 7583 679 87.6 globlastp 4151oak|10v1|SRR039734S0086911_P1 7583 679 87.6 globlastp 4152oak|10v1|SRR039736S0009196_P1 7583 679 87.6 globlastp 4153oak|10v1|SRR039736S0072306_P1 7583 679 87.6 globlastp 4154oak|10v1|SRR039740S0045447_P1 7583 679 87.6 globlastp 4155orange|11v1|AF312227_P1 7590 679 87.6 globlastp 4156papaya|gb165|EX248933_P1 7591 679 87.6 globlastp 4157tripterygium|11v1|sRR098677X103515 7592 679 87.6 globlastp 4158poplar|13v1|BI069694_P1 7593 679 87.6 globlastp 4159scabiosa|11v1|SRR063723X100037 7594 679 87.5 globlastp 4160scabiosa|11v1|SRR063723X103515 7595 679 87.5 globlastp 4161clementine|11v1|AF312227_P1 7596 679 87.3 globlastp 4162sesame|12v1|SESI12V1397389 7597 679 87.3 globlastp 4163flaveria|11v1|SRR149232.287040_T1 7598 679 87.22 glotblastn 4164flaveria|11v1|SRR149232.443407_T1 7599 679 87.22 glotblastn 4165oak|10v1|FN55430_T1 7600 679 87.22 glotblastn 4166oak|10v1|SRR006309S0041356_P1 7601 679 87.22 glotblastn 4167peach|gb157.2|BU039212_P1 7602 679 87.2 globlastp 4168prunus_mume|13v1|BI203091_P1 7603 679 87.2 globlastp 4169ambrosia|11v1|SRR346935.117253_P1 7604 679 87.2 globlastp 4170ambrosia|11v1|SRR346935.147253_P1 7605 679 87.2 globlastp 4171apple|11v1|CN861354_P1 7606 679 87.2 globlastp 4172catjaramtjis|11v1|EG554220_P1 7607 679 87.2 globlastp 4173flaveria|11v1|SRR149229.166261_P1 7608 679 87.2 globlastp 4174fraxinus|11v1|sRR058827.100981_P1 7609 679 87.2 globlastp 4175onion|12v1|CF435462_P1 7610 679 87.2 globlastp 4176 poplar|10v1|BI0696947611 679 87.2 globlastp 4177 primula|11v1|sRR098679X101097_P1 7612 67987.2 globlastp 4178 prunus|10v1|BI203091 7602 679 87.2 globlastp 4179sunflower|12v1|CD846630 7613 679 87.2 globlastp 4180tobacco|gb162|CO046503 7614 679 87.2 globlastp 4181tobacco|gb162|CV015951 7615 679 87.2 globlastp 4182flaveria|11v1|SRR149229.102100_T1 7616 679 87.17 glotblastn 4183oak|10v1|DB998558_P1 7617 679 86.9 globlastp 4184 oak|10v1|FP025055_P17617 679 86.9 globlastp 4185 flaveria|11v1|SRR149241.235250_T1 7618 67986.84 glotblastn 4186 olea|13v1|SRR014463X10518D1_P1 7619 679 86.8globlastp 4187 ambrosia|11v1|SRR346935.103321_P1 7620 679 86.8 globlastp4188 apple|11v1|CN493989_P1 7621 679 86.8 globlastp 4189artemisia|10v1|EY033550_P1 7622 679 86.8 globlastp 4190chelidonium|11v1|SRR084752X101005_P1 7623 679 86.8 globlastp 4191cotton|11v1|AI055533_P1 7624 679 86.8 globlastp 4192cotton|11v1|CA992753_P1 7625 679 86.8 globlastp 4193cotton|11v1|SRR032799.214106_P1 7626 679 86.8 globlastp 4194dandelion|10v1|DY815081_P1 7627 679 86.8 globlastp 4195flaveria|11v1|SRR149232.100184_P1 7628 679 86.8 globlastp 4196flaveria|11v1|SRR149232.140133_P1 7629 679 86.8 globlastp 4197flaveria|11v1|SRR149232.26769_P1 7629 679 86.8 globlastp 4198flaveria|11v1|SRR149238.14417_P1 7629 679 86.8 globlastp 4199flaveria|11v1|SRR149238.21025_P1 7630 679 86.8 globlastp 4200flaveria|11v1|SRR149241.107004_P1 7629 679 86.8 globlastp 4201gossypium_raimondii|12v1|AI055533_P1 7624 679 86.8 globlastp 4202gossypium_raimondii|12v1|CA992753_P1 7631 679 86.8 globlastp 4203kiwi|gb166|FG403363_P1 7632 679 86.8 globlastp 4204nicotiana_benthamiana|12v1|CN655121_P1 7633 679 86.8 globlastp 4205nicotiana_benthamiana|gb162|CN655121 7633 679 86.8 globlastp 4206olea|11v1|SRR014463.10518 7634 679 86.8 globlastp 4207petunia|gb171|CV299277_P1 7635 679 86.8 globlastp 4208potato|10v1|BI405771_P1 7636 679 86.8 globlastp 4209solanum_phureja|09v1|sPHTOMCAB4A 7636 679 86.8 globlastp 4210tobacco|gb162|AY219853 7637 679 86.8 globlastp 4211tripterygium|11v1|SRR098677X100129 7638 679 86.8 globlastp 4212walnuts|gb166|EL890970 7639 679 86.8 globlastp 4213watermelon|11v1|AA660116 7640 679 86.8 globlastp 4214watermelon|11v1|DV632407 7640 679 86.8 globlastp 4215eggplant|10v1|FS008108_P1 7641 679 86.7 globlastp 4216oak|10v1|FN726305_P1 7642 679 86.6 globlastp 4217 oak|10v1|FN733084_P17642 679 86.6 globlastp 4218 oak|10v1|FP041636_P1 7642 679 86.6globlastp 4219 oak|10v1|SRR039739S0093167_T1 7643 679 86.57 glotblastn4220 ambrosia|11v1|GW917892_P1 7644 679 86.5 globlastp 4221artemisia|10v1|GW328360_P1 7645 679 86.5 globlastp 4222artemisia|10v1|SRR019254S0010163_P1 7646 679 86.5 globlastp 4223cleome_gynandra|10v1|SRR015532S0001436_P1 7647 679 86.5 globlastp 4224cotton|11v1|CO76370_P1 7648 679 86.5 globlastp 4225cotton|11v1|CO88170_P1 7649 679 86.5 globlastp 4226euonymus|11v1|sRR070038X100346_P1 7650 679 86.5 globlastp 4227euonymus|11v1|sRR070038X126789_P1 7650 679 86.5 globlastp 4228fraxinus|11v1|SRR058827.78550_P1 7651 679 86.5 globlastp 4229gossypium_raimondii|12v1|EX167041_P1 7648 679 86.5 globlastp 4230humulus|11v1|ES437745_P1 7652 679 86.5 globlastp 4231monkeyflower|10v1|DV206930 7653 679 86.5 globlastp 4232parthenium|10v1|GW777165_P1 7654 679 86.5 globlastp 4233phyla|12v2|SRR099035X122028_P1 7655 679 86.5 globlastp 4234primula|11v1|SRR098679X100066_P1 7656 679 86.5 globlastp 4235primula|11v1|SRR098679X10006_P1 7657 679 86.5 globlastp 4236primula|11v1|SRR098679X100570_P1 7656 679 86.5 globlastp 4237primula|11v1|SRR098679X116775_P1 7656 679 86.5 globlastp 4238tobacco|gb162|BU673944 7658 679 86.5 globlastp 4239nicotiana_benthamiana|12v1|AY219853_P1 7659 679 86.5 globlastp 4240castorbean|11v1|EE254310 7660 679 86.47 glotblastn 4241castorbean|12v1|EE254128_T1 7660 679 86.47 glotblastn 4242flaveria|11v1|SRR149239.140984_T1 7661 679 86.47 glotblastn 4243flaveria|11v1|SRR149241.127580XX1_T1 7662 679 86.47 glotblastn 4244flaveria|11v1|SRR149229.10104_T1 7663 679 86.42 glotblastn 4245artesia|10v1|EY031992_P1 7664 679 86.4 globlastp 4246beet|12v1|Y13865_P1 7665 679 86.4 globlastp 4247blueberry|12v1|SRR353282X11622D1_P1 7666 679 86.4 globlastp 4248hornbeam|12v1|SRR364455.101039_P1 7667 679 86.4 globlastp 4249wheat|12v3|TA12V3CRP193058 7665 679 86.4 globlastp 4250blueberry|12v1|SRR353282X14069D1_T1 7668 679 86.36 glotblastn 4251leymus|gb166|DC808679_P1 7669 679 86.3 globlastp 4252flaveria|11v1|SRR149232.118398_P1 7670 679 86.2 globlastp 4253oak|10v1|sRR039742S0071982_T1 7671 679 86.19 glotblastn 4254apple|11v1|CV128668_T1 7672 679 86.14 glotblastn 4255amsonia|11v1|SRR098688X118176_P1 7673 679 86.1 globlastp 4256cirsium|11v1|SRR346952.11999_P1 7674 679 86.1 globlastp 4257coffea|10v1|DV663455_P1 7675 679 86.1 globlastp 4258euonymyus|11v1|SRR070038X104689_P1 7676 679 86.1 globlastp 4259euonymyus|11v1|SRR070038X117540_P1 7677 679 86.1 globlastp 4260euonymyus|11v1|SRR070038X120123_P1 7678 679 86.1 globlastp 4261ginseng|10v1|AB236867_P1 7679 679 86.1 globlastp 4262ipomoea_batatas|10v1|CB330898_P1 7680 679 86.1 globlastp 4263nicotiana_benthamiana|12v1|CN655392_P1 7681 679 86.1 globlastp 4264nicotiana_benthamiana|gb162|CN655392 7681 679 86.1 globlastp 4265nicotiana_benthamiana|gb162|CN744088 7682 679 86.1 globlastp 4266pepper|12v1|BM059797_P1 7683 679 86.1 globlastp 4267pteridium|11v1|SRR043594X100088 7684 679 86.1 globlastp 4268spureg|gb161|BG467374 7685 679 86.1 globlastp 4269strawberry|11v1|CO816850 7686 679 86.1 globlastp 4270tomato|11v1|ToMCAB4A 7687 679 86.1 globlastp 4271flaveria|11v1|SRR149232.106135_T1 7688 679 86.09 glotblastn 4272flaveria|11v1|SRR149244.184810_T1 7689 679 86.09 glotblastn 4273primula|11v1|SRR098679X102577_T1 7690 679 86.09 glotblastn 4274nicotiana_benthamiana|12v1|CN742096_P1 7691 679 86 globlastp 4275nicotiana_benthamiana|gb162|CN742025 7691 679 86 globlastp 4276flaveria|11v1|SRR149232.261626_T1 7692 679 85.71 glotblastn 4277gossypium_raimondii|12v1|SRR032881.377381_T1 7693 679 85.71 glotblastn4278 amborella|12v3|FD430431_P1 7694 679 85.7 globlastp 4279arnica|11v1|SRR099034X100993_P1 7695 679 85.7 globlastp 4280arnica|11v1|SRR099034X104794_P1 7696 679 85.7 globlastp 4281artemisia|10v1|EY036084_P1 7697 679 85.7 globlastp 4282b_juncea|12v1|E6ANDIZ01A5Z1E_P1 7698 679 85.7 globlastp 4283b_oleracea|gb161|DY029975_P1 7699 679 85.7 globlastp 4284beech|11v1|DT317612_P1 7700 679 85.7 globlastp 4285canola|11v1|CN730788_P1 7699 679 85.7 globlastp 4286cotton|11v1|CO073198_P1 7701 679 85.7 globlastp 4287eucalyptus|11v2|ES588412_P1 7702 679 85.7 globlastp 4288gossypium_raimondii|12v1|BM359605_P1 7701 679 85.7 globlastp 4289ipomoea_nil|10v1|BJ555028_P1 7703 679 85.7 globlastp 4290lotus|09v1|AI967689_P1 7704 679 85.7 globlastp 4291nasturtium|11v1|SRR032558.110591_P1 7705 679 85.7 globlastp 4292plantago|11v1|SRR066373X153324_P1 7706 679 85.7 globlastp 4293platanus|11v1|sRR096786X100930_P1 7707 679 85.7 globlastp 4294radish|gb164|EV539160 7708 679 85.7 globlastp 4295 rose|12v1|BQ1054197709 679 85.7 globlastp 4296 soybean|11v1|GLYMA02G47560 7710 679 85.7globlastp 4297 soybean|12v1|GLYMA02G47560_P1 7710 679 85.7 globlastp4298 tragopogon|10v1|SRR020205S0000345 7711 679 85.7 globlastp 4299zamia|gb166|DY031222 7712 679 85.61 glotblastn 4300switchgrass|gb167|DN144083 7713 679 85.6 globlastp 4301wheat|12v3|BJ243829 7714 679 85.55 glotblastn 4302triphysaria|10v1|EX982551 7715 679 85.4 globlastp 4303wheat|12v3|BE213396 7716 679 85.4 globlastp 4304flaveria|11v1|SRR149240.400347_T1 7717 679 85.34 glotblastn 4305b_juncea|12v1|E6ANDIZ01A1QOA_P1 7718 679 85.3 globlastp 4306b_juncea|12v1|E6ANDIZ01A1X7Z_P1 7719 679 85.3 globlastp 4307b_rapa|11v1|CN729524_P1 7718 679 85.3 globlastp 4308banana|12v1|DN238651_P1 7720 679 85.3 globlastp 4309flaveria|11v1|SRR149232.100433_P1 7721 679 85.3 globlastp 4310oil_palm|11v1|SRR190698.104272_P1 7722 679 85.3 globlastp 4311onion|12v1|CF435798_P1 7723 679 85.3 globlastp 4312potato|10v1|AW096885_P1 7724 679 85.3 globlastp 4313sarracenia|11v1|SRR192669.104211 7725 679 85.3 globlastp 4314solanum_phureja|09v1|SPHTOMCAB5A 7726 679 85.3 globlastp 4315tomato|11v1|ToMCAB5A 7727 679 85.3 globlastp 4316flaveria|11v1|SRR149232.101185_P1 7728 679 85.2 globlastp 4317b_juncea|12v1|E6ANDIZ01A12AY_P1 7729 679 85 globlastp 4318b_juncea|12v1|E6ANDIZ01A1WNX_P1 7730 679 85 globlastp 4319b_rapa|11v1|H07776_P1 7730 679 85 globlastp 4320 canola|11v1|DQ068137_P17731 679 85 globlastp 4321 cleome_spinosa|10v1|sRR05531S0000194_P1 7732679 85 globlastp 4322 cucuma|10v1|DY384030_P1 7733 679 85 globlastp 4323euphorbia|11v1|SRR098678X11662_P1 7734 679 85 globlastp 4324flax|11v1|CV478200_P1 7735 679 85 globlastp 4325ginger|gb164|DY345128_P1 7736 679 85 globlastp 4326pepper|12v1|BM064264_P1 7737 679 85 globlastp 4327phalaenopsis|11v1|SRR125771.1001461_P1 7738 679 85 globlastp 4328phalaenopsis|11v1|SRR125771.1037297_P1 7738 679 85 globlastp 4329phalaenopsis|11v1|SRR125771.1105138XX1_P1 7739 679 85 globlastp 4330pigeonpea|11v1|GR472384_P1 7740 679 85 globlastp 4331silene|11v1|SRR096785X165309 7741 679 85 globlastp 4332soybean|11v1|GLYMA14G01130 7742 679 85 globlastp 4333soybean|12v1|GLYMA14G01130_P1 7742 679 85 globlastp 4334thellungiella_parvulum|11v1|DN773146 7743 679 85 globlastp 4335vinca|11v1|SRR098690X102339 7744 679 85 globlastp 4336flaveria|11v1|SRR149232.121548_T1 7745 679 84.96 glotblastn 4337platanus|11v1|SRR096786X100804_T1 7746 679 84.91 glotblastn 4338podocarpus|10v1|SRR065014S0000148_P1 7747 679 84.9 globlastp 4339tamarix|gb166|CF199718 7748 679 84.9 globlastp 4340bean|12v2|CB280487_P1 7749 679 84.6 globlastp 4341arabidopsis_lyrata|09v1|JGIAL011472_P1 7750 679 84.6 globlastp 4342arabidopsis_lyrata|09v1|JGIAL016921_P1 7751 679 84.6 globlastp 4343arabidopsis|1-v1|AT2G05070_P1 7752 679 84.6 globlastp 4344arabidopsis|1-v1|AT2G05100_P1 7753 679 84.6 globlastp 4345arabidopsis|1-v1|AT3G27690_P1 7754 679 84.6 globlastp 4346b_juncea|12v1|E6ANDIZ01A03AV_P1 7755 679 84.6 globlastp 4347b_juncea|12v1|E6ANDIZ01A0Z2K_P1 7756 679 84.6 globlastp 4348b_juncea|12v1|E6ANDIZ01A1N6D_P1 7755 679 84.6 globlastp 4349b_juncea|12v1|E6ANDIZ01A1NND_P1 7757 679 84.6 globlastp 4350b_juncea|12v1|E6ANDIZ01A1R82_P1 7755 679 84.6 globlastp 4351b_juncea|12v1|E6ANDIZ01A1TII_P1 7755 679 84.6 globlastp 4352b_juncea|12v1|E6ANDIZ01A306F_P1 7758 679 84.6 globlastp 4353b_juncea|12v1|E6ANDIZ01A3850_P1 7755 679 84.6 globlastp 4354b_rapa|11v1|DC841551_P1 7755 679 84.6 globlastp 4355b_rapa|11v1|H07528_P1 7755 679 84.6 globlastp 4356 bean|12v1|CB2804877749 679 84.6 globlastp 4357 canola|11v1|CN728926_P1 7759 679 84.6globlastp 4358 canola|11v1|CN735221_P1 7755 679 84.6 globlastp 4359canola|11v1|EE498384_P1 7760 679 84.6 globlastp 4360cowpea|12v1|AF279248_P1 7761 679 84.6 globlastp 4361flax|11v1|EH791162XX1_P1 7762 679 84.6 globlastp 4362peanut|10v1|CD037580_P1 7763 679 84.6 globlastp 4363primula|11v1|SRR098679X100468_P1 7764 679 84.6 globlastp 4364radish|gb164|EV566349 7765 679 84.6 globlastp 4365tabernaemontana|11v1|SRR098689X100234 7766 679 84.6 globlastp 4366thellungiella_parvulum|11v1|EPCRP010282 7767 679 84.6 globlastp 4367trigonella|11v1|SRR066194X107056 7768 679 84.6 globlastp 4368vinca|11v1|SRR098690X102987 7769 679 84.6 globlastp 4369bruguiera|gb166|BP938701_T1 7770 679 84.59 glotblastn 4370euphorbia|11v1|BG467374_T1 7771 679 84.59 glotblastn 4371flaveria|11v1|SRR149232.382755_T1 7772 679 84.59 glotblastn 4372distylium|11v1|SRR065077X100508_P1 7773 679 84.5 globlastp 4373rye|12v1|DRR001012.124233 7774 679 84.41 glotblastn 4374pseudoroegneria|gb167|FF344287 7775 679 84.4 globlastp 4375b_juncea|12v1|E6ANDIZ01A030U_P1 7776 679 84.3 globlastp 4376b_juncea|12v1|E6ANDIZ01A3ULG_P1 7777 679 84.3 globlastp 4377canola|11v1|CN728765_P1 7778 679 84.3 globlastp 4378canola|11v1|H07668_P1 7778 679 84.3 globlastp 4379phalaenopsis|11v1|SRR125771.1028146_T1 7779 679 84.27 glotblastn 4380acacia|10v1|GR481547_P1 7780 679 84.2 globlastp 4381canola|11v1|DW998983_P1 7781 679 84.2 globlastp 4382clover|gb162|BB902546_P1 7782 679 84.2 globlastp 4383medicago|12v1|AW698691_P1 7783 679 84.2 globlastp 4384pea|11v1|X06822_P1 7784 679 84.2 globlastp 4385 tamarix|gb166|EH0484877785 679 84.15 glotblastn 4386 conzya|10v1|SRR035294S0000873_P1 7786 6798.1 globlastp 4387 nasturtium|11v1|SRR032558.100687_P1 7787 679 84.1globlastp 4388 flaveria|11v1|SRR149229.203075_T1 7788 679 84.03glotblastn 4389 b_juncea|12v1|E6ANDIZ01B5EYF_P1 7789 679 83.9 globlastp4390 phalaenopsis|11v1|SRR125771.1067548_T1 7790 679 83.9 glotblastn4391 thellungiella_halophilum|11v1|DN773488 7791 679 83.9 globlastp 4392b_rapa|11v1|H07520_T1 7792 679 83.83 glotblastn 4393flaveria|11v1|SRR149241.1012_T1 7793 679 83.83 glotblastn 4394chickpea|13v2|FE670525_P1 7794 679 83.8 globlastp 4395chickpea|13v2|SRR133517.116766_P1 7794 679 83.8 globlastp 4396zostera|12v1|AM766581_P1 7795 679 83.8 globlastp 4397zostera|12v1|AM766700_P1 7796 679 83.8 globlastp 4398zostera|12v1|AM767071_P1 7796 679 83.8 globlastp 4399zostera|12v1|AM771182_P1 7796 679 83.8 globlastp 4400zostera|12v1|AM772561_P1 7796 679 83.8 globlastp 4401zostera|12v1|SRR0575351X10159D1_P1 7796 679 83.8 globlastp 4402zostera|12v1|SRR0575351X113049D1_P1 7796 679 83.8 globlastp 4403zostera|12v1|SRR0575351X12377D1_P1 7796 679 83.8 globlastp 4404zostera|12v1|SRR0287819X38286D1_P1 7796 679 83.8 globlastp 4405zostera|12v1|SRR287819X192166D1_P1 7796 679 83.8 globlastp 4406zostera|12v1|SRR0575351X20043D1_P1 7796 679 83.8 globlastp 4407zostera|12v1|SRR0575351X384031D1_P1 7796 679 83.8 globlastp 4408zostera|12v1|SRR0575351X497879D1_P1 7796 679 83.8 globlastp 4409zostera|12v1|SRR0287819X138665D1_P1 7796 679 83.8 globlastp 4410zostera|12v1|SRR287819X35431D1_P1 7796 679 83.8 globlastp 4411chickpea|11v1|SRR133517.1121 7794 679 83.8 globlastp 4412pine|10v2|H75042_P1 7797 679 83.8 globlastp 4413pseudotsuga|10v1|SRR065119S0008709 7798 679 83.8 globlastp 4414silene|11v1|DV768290 7799 679 83.8 globlastp 4415 zostera|10v1|AM7665817796 679 83.8 globlastp 4416 chickpea|13v2|FE668437_P1 7794 679 83.8globlastp 4417 zostera|12v1|SRR0575351X182694D1_T1 7800 679 83.77glotblastn 4418 tripterygium|11v1|SRR098677X347520 7801 679 83.71glotblastn 4419 radish|gb164|EW715183 7802 679 83.58 glotblastn 4420b_rapa|11v1|CB686314_T1 7803 679 83.52 glotblastn 4421canola|11v1|H07528_T1 7804 679 86.52 glotblastn 4422canola|11v1|SRR019558.17346_T1 7805 679 83.52 glotblastn 4423chickpea|13v2|FE668894_P1 7806 679 83.5 globlastp 4424chickpea|13v2|SRR133517.1121_P1 7806 679 83.5 globlastp 4425aquilegia|10v2|DR947630_P1 7807 679 83.5 globlastp 4426spruce|11v1|AF247178XX2 7808 679 83.46 glotblastn 4427zostera|12v1|SRR0575351X382862D1_T1 7809 679 83.4 glotblastn 4428zostera|12v1|SRR0575351X453918D1_T1 7810 679 83.4 glotblastn 4429zostera|12v1|SRR287819X118789D1_T1 7811 679 83.4 glotblastn 4430zostera|12v1|SRR287819X139486D1_T1 7812 679 83.4 glotblastn 4431iceplant|gb164|BE034672_P1 7813 679 83.4 globlastp 4432epimedium|11v1|SRR013502.1002_P1 7814 679 83.3 globlastp 4433flax|11v1|JG103153_P1 7815 679 83.3 globlastp 4434senecio|gb170|CO553206 7819 679 83.3 globlastp 4435cedrus|11v1|SRR065007X101826_P1 7817 679 83.1 globlastp 4436euphorbia|11v1|DV116721_P1 7818 679 83.1 globlastp 4437maritime_pine|10v1|AL749749_P1 7819 679 83.1 globlastp 4438phalaenopsis|11v1|SRR125771.1001581_P1 7821 679 83.1 globlastp 4439poppy|11v1|SRR030259.10434_P1 7821 679 83.1 globlastp 4440poppy|11v1|SRR030259.230238_P1 7821 679 83.1 globlastp 4441poppy|11v1|SRR030259.77777XX2_P1 7821 679 83.1 globlastp 4442poppy|11v1|SRR030259.83862_P1 7821 679 83.1 globlastp 4443spruce|11v1|ES667439XX2 7822 679 83.1 globlastp 4444chickpea|13v2|FE670924_T1 7823 679 83.08 glotblastn 4445spruce|11v1|AF247178XX1 7824 679 83.08 glotblastn 4446zostera|12v1|SRR0575351X345894D1_T1 7825 679 83.02 glotblastn 4447zostera|12v1|SRR0575351X495982D1_T1 7826 679 83.02 glotblastn 4448zostera|12v1|SRR287819X80644D1_T1 7827 679 83.02 glotblastn 4449zostera|12v1|SRR287819X117482D1_T1 7828 679 83.02 glotblastn 4450taxus|10v1|SRR032523S0000608 7829 679 83 globlastp 4451flaveria|11v1|SRR149232.103725_T1 7830 679 82.95 glotblastn 4452valeriana|11v1|SRR099041X150486 7831 679 82.9 globlastp 4453chichorium|gb171|EH694526_T1 7832 679 82.89 glotblastn 4454flaveria|11v1|SRR149229.460712_T1 7833 679 82.89 glotblastn 4455flaveria|11v1|SRR149238.103830_T1 7834 679 82.89 glotblastn 4456b_rapa|11v1|L33601_T1 7835 679 82.84 glotblastn 4457abies|11v2|SRR098676X10263_P1 7836 679 82.8 globlastp 4458b_juncea|12v1|E6ANDIZ01AO4N7_P1 7837 679 82.8 globlastp 4459cedrus|11v1|SRR065007X10066_P1 7838 679 82.8 globlastp 4460artemisia|10v1|EY043666_T1 7839 679 82.71 glotblastn 4461spruce|11v1|ES8666284XX1 7840 679 82.71 glotblastn 4462cedrus|11v1|SRR065007X100115_P1 7841 679 82.7 globlastp 4463cedrus|11v1|SRR065007X100351_P1 7842 679 82.7 globlastp 4464cedrus|11v1|SRR065007X102605XX1_P1 7841 679 82.7 globlastp 4465maritime_pine|10v1|BX681281_P1 7841 679 82.7 globlastp 4466pine|10v2|AL749749_P1 7843 679 82.7 globlastp 4467 pine|10v2|X13407_P17844 679 82.7 globlastp 4468 poppy|11v1|SRR030260.101101_P1 7845 67982.7 globlastp 4469 pseudotsuga|10v1|SRR065119S0002848 7846 679 82.7globlastp 4470 spruce|11v1|ES245009XX2 7847 679 82.7 globlastp 4471zostera|12v1|SRR287819X106739D1_T1 7848 679 82.64 glotblastn 4472blueberry|12v1|SRR353282X1205D1_P1 7849 679 82.6 globlastp 4473cephalotaxus|11v1|sRR0643282X1205D1_P1 7850 679 82.6 globlastp 4474epimedium|11v1|SRR013502.1083_P1 7851 679 82.6 globlastp 4475poppy|11v1|SRR030259.16930_P1 7852 679 82.6 globlastp 4476zostera|12v1|SRR287819X116036D1_T1 7853 679 82.51 glotblastn 4477cycas|gb166|CB089403_P1 7854 679 82.5 globlastp 4478flaveria|11v1|SRR149229.105154_P1 7855 679 82.5 globlastp 4479flaveria|11v1|SRR149229.128816_P1 7855 679 82.5 globlastp 4480silene|11v1|SIPCAB 7856 679 82.5 globlastp 4481thellungiella_halophilum|11v1|DN773146 — 679 82.46 glotblastn 4482abies|11v2|SRR098676X103819_P1 7857 679 82.4 globlastp 4483b_juncea|12v|E6ANDIZ01A5T96_P1 7858 679 82.4 globlastp 4484poppy|11v1|SRT030260.126583XX2_T1 7859 679 82.33 glotblastn 4485spruce|11v1|GT885731 7860 679 82.33 glotblastn 4486flaveria|11v1|SRR149239.62768_P1 7861 679 82.3 globlastp 4487maritime_pin|10v1|AJ309102_P1 7862 679 82.3 globlastp 4488poppy|11v1|SRR030259.10584_P1 7863 679 82.3 globlastp 4489poppy|11v1|SRR030261.48143_P1 7864 679 82.3 globlastp 4490poppy|11v1|SRR030265.257384_P1 7865 679 82.3 globlastp 4491spruce|11v1|ES245009XX1 7866 679 82.3 globlastp 4492spruce|11v1|ES667997XX1 7867 679 82.3 globlastp 4493spruce|11v1|FD739232XX2 7868 679 82.3 globlastp 4494zostera|12v1|SRR287822X109188D1_T1 7869 679 82.26 glotblastn 4495zostera|12v1|SRR287822X91863D1_T1 7870 679 82.26 glotblastn 4496zostera|12v1|SRR287908X123712D1_T1 7871 679 82.26 glotblastn 4497flax|11v1|JG103521_P1 7872 679 82.2 globlastp 4498sequoia|10v1|SRR065044S0005034 7873 679 82.2 globlastp 4499flaveria|11v1|SRR149241.121618_T1 7874 679 82.13 glotblastn 4500flaveria|11v1|SRR149241.14885_T1 7875 679 82.13 glotblastn 4501oak|10v1|FP025890_T1 7876 679 82.13 glotblastn 4502spruce|11v1|ES667073XX1 7877 679 81.95 glotblastn 4503spruce|11v1|ES667273XX2 7878 679 81.95 glotblastn 4504flaveria|11v1|SRR149232.223435_T1 7879 679 81.95 glotblastn 4505ambrosia|11v1|SRR346943.117749_P1 7880 679 81.82 glotblastn 4506oak|10v1|FP025574_P1 7881 679 81.7 globlastp 4507spikemoss|gb165|DN838028 7882 679 81.7 globlastp 4508zostera|12v1|SRR057351X785286D1_P1 7883 679 81.65 glotblastn 4509zostera|12v1|SRR287822X123050D1_P1 7883 679 81.6 globlastp 4510zostera|12v1|SRR287828X1935D1_P1 7884 679 81.6 globlastp 4511centaurea|11v1|EH725583_P1 7885 679 81.6 globlastp 4512poppy|11v1|SRR030259.128803_P1 7886 679 81.6 globlastp 4513senecio|gb170|DY659329 7887 679 81.6 globlastp 4514spruce|11v1|EX396361XX1 7888 679 81.6 globlastp 4515sequoia|10v1|SRR065044S0052503 7889 679 81.41 glotblastn 4516monkeyflower|12v|DV206485_P1 7890 679 81.4 globlastp 4517spruce|11v1|EX424733XX1 7891 679 81.37 glotblastn 4518spruce|11v1|ES661748XX1 7892 679 81.3 glotblastn 4519chickpea|13v2|SRR133521.241946_T1 7893 679 81.2 glotblastn 4520artemisia|10v1|EY065972_T1 7894 679 81.2 glotblastn 4521cedrus|11v1|SRR065007X100555_T1 7895 679 81.2 glotblastn 4522fern|gb171|DK944111_P1 7896 679 81.2 globlastp 4523pine|10v2|PPU51634_T1 7897 679 81.2 glotblastn 4524pteridium|11v1|SRR043594X100886 7898 679 81.2 globlastp 4525cenchrus|gb166|EB670451_P1 7899 679 81.1 globlastp 4526pteridium|11v1|GW57843 7900 679 81.1 globlastp 4527euphorbia|11v1|SRR098678X113578_T1 7901 679 81.06 glotblastn 4528euphorbia|11v1|SRR098678X18309_T1 7902 679 81.06 glotblastn 4529artemisia|10v1|EY045804_P1 7903 679 81 globlastp 4530zostera|12v1|SRR287908X101490D1_T1 7904 679 80.97 glotblastn 4531canola|11v1|EG020467_T1 7905 679 80.9 glotblastn 4532chickpea|13v2|SRR133517.104734_T1 7906 679 80.83 glotblastn 4533cynara|gb167|GE591421_T1 7907 679 80.83 glotblastn 4534zostera|12v1|SRR287819X123850D1_T1 7908 679 80.75 glotblastn 4535cryptomeria|gb166|BW994897_P1 7909 679 80.7 globlastp 4536flaveria|11v1|SRR149238.332042_T1 7910 679 80.61 glotblastn 4537radish|gb164|EV542239 7911 679 80.61 glotblastn 4538chickpea|13v2|SRR133517.100453_P1 7912 679 80.5 globlastp 4539zostera|12v1|SRR287819X52076D1_T1 7913 679 80.45 glotblastn 4540zostera|12v1|SRR287819X57215D1_T1 7914 679 80.23 glotblastn 4541phalaenopsis|11v1|SRR125771.1148769_T1 7915 679 80.23 glotblastn 4542spruce|11v1|EES262162XX1 7916 679 80.23 glotblastn 4543euphorbia|11v1|DV137035_P1 7917 679 80.2 globlastp 4543spurge|gb161|DV137035 7917 679 80.2 globlastp 4544nasturtium|11v1|SRR032558.14184_P1 7918 679 80.2 globlastp 4545chickpea|13v2|SRR133517.105445 7919 679 80.08 glotblastn 4546flaveria|11v1|SRR149238.123511_T1 7920 679 80.08 glotblastn 4547strawberry|11v1|EX663159 7921 679 80.08 glotblastn 4548zostera|12v1|SRR287828X38216D1_T1 7922 679 80 glotblastn 4549barley|12v1|CX631446_P1 7923 681 85 globlastp 4550foxtail_millet|11v3|PHY7SI009670M_P1 7924 681 83.8 globlastp 4551rice|11v1|D48912 7925 681 83.7 globlastp 4552 rye|12v1|DRR001012.1434347926 681 83.4 globlastp 4553 sorghum|12v1|SB06G017470 7927 681 82.2globlastp 4554 switchgrass|12v1|SRR187767.630949_P1 7928 681 82globlastp 4555 switchgrass|12v1|FL916996_P1 7929 681 81.2 globlastp 4556maize|10v1|BG833646_T1 7930 681 80.69 glotblastn 4557foxtail_millet|11v3|PHY67SI036906M_P1 7931 682 82.7 globlastp 4558switchgrass|12v1|DN140973_P1 7932 682 82.3 globlastp 4559switchgrass|gb167|DN140777 7933 682 81.9 globlastp 4560switchgrass|12v1|DN140777_P1 7934 682 81.6 globlastp 4561switchgrass|gb167|DN140973 7935 682 81 glotblastn 4562millet|10v1|CD725024_P1 7936 682 80.5 globlastp 4563solanum_phureja|09v1|SPHAW154852 7937 683 89.4 globlastp 4564eggplant|10v1|FS004080_P1 7938 683 83.8 globlastp 4565wheat|12v3|BE443100 7939 693 95.7 globlastp 4566ye|12v1|DRR001012.168798 7940 693 92.8 globlastp 4567foxtail_millet|11v3|PHY7SI022926M_T1 7941 693 82.89 glotblastn 4568rice|11v1|D40052 7942 693 81.3 globlastp 4569 switchgrass|gb167FE6421047943 693 81.2 globlastp 4570 switchgrass|12v1|FL863086_T1 7944 693 81.01glotblastn 4571 foxtail_millet|11v3|PHY7SI022926M_T1 7945 693 80.4globlastp 4572 sorghum|12v1|SB03G044860 7946 693 80.08 glotblastn 4573pseudoroegneria|gb167|FF342114 7947 694 81.8 globlastp 4574wheat|12v3|SRR073321X137372D1 7948 695 92.38 glotblastn 4575wheat|12v3SRR400820X100753D1 7949 695 92.38 glotblastn 4576wheat|12v3|CA740981 7950 695 91.43 glotblastn 4577 wheat|12v3|AL8109017951 695 90.48 glotblastn 4578 wheat|12v3|CJ543873 7952 695 89.52glotblastn 4579 wheat|12v3|SRR073322X395399D1 7953 695 89.52 glotblastn4580 wheat|12v3|CD920952 7954 695 86.67 glotblastn 4581rye|12v1|DRR001012.620799 7955 695 85.71 glotblastn 4582pseudoroegneria|gb167|FF344783 7956 695 84.1 globlastp 4583wheat|12v3|CA649000 7957 696 83.05 glotblastn 4584 wheat|12v3|CJ5403767958 696 81.88 glotblastn 4585 oat|11v1|GR351065_T1 7959 697 88.04glotblastn 4586 rice|11v1|AT003532 7960 697 87.5 glotblastn 4587foxtail_millet|11v3|PHY7SI023571M_T1 7961 697 85.1 glotblastn 4588maize|10v1|AW455707_T1 7962 697 85.1 glotblastn 4589sugarcane|10v1|CA075702 7963 697 84.62 glotblastn 4590switchgrass|gb167|DN145223 7964 697 84.13 glotblastn 4591switchgrass|gb167|DN145223_T1 7965 697 83.65 glotblastn 4592sorghum|12v1|SB08G013180 7966 697 83.65 glotblastn 4593millet|10v1|EVO454PM010844_T1 7967 697 83.17 glotblastn 4594switchgrass|12v1|FE598880_T1 7968 697 82.21 glotblastn 4595rice|11v1|BI804923 7969 700 84.93 glotblastn 4596brachypodium|12v1|BRADI1G58250_T1 7970 700 84.08 glotblastn 4597wheat|12v3|BQ172325 7971 700 84.07 glotblastn 4598switchgrass|12v1|HO258167_T1 7972 700 82.48 glotblastn 4599foxtail_millet|11v3|EC511896_P1 7973 701 94.1 globlastp 4600switchgrass|12v1|FE633580_P1 7974 701 89.3 globlastp 4601switchgrass|gb167|FE633580 7975 701 82.1 globlastp 4602brachypodium|12v1|BRADI1G01870_T1 7976 701 80.37 glotblastn 4603sorghum|12v1|SB06G032510 7977 702 88.5 globlastp 4604switchgrass|12v1|SRR187769.104771_P1 7978 702 87.8 globlastp 4605maize|10v1|CD949464_P1 7979 702 86.5 globlastp 4606sorghum|12v1|SB06G032490 7980 702 86.5 globlastp 4607wheat|12v3|CA617374 7981 702 83.3 globlastp 4608switchgrass|12v1|FE622730_P1 7982 703 86.2 globlastp 4609foxtail_millet|11v3|PHY7SI029253M_T1 7983 703 86.08 glotblastn 4610switchgrass|12v1|FL727202_T1 7984 703 85.46 glotblastn 4611millet|10v1|EVO454PM0027881_P1 7985 703 84.1 globlastp 4612sugarcane|10v1|CA068859 704 704 100 globlastp 4613sorghum|12v1|SB08G019790 7986 704 99.4 globlastp 4614foxtail_millet|11v3|EC612562_P1 7987 704 96.7 globlastp 4615switchgrass|12v1|DN141026_P1 7988 704 96.1 globlastp 4616switchgrass|gb167|DN141026 7988 704 96.1 globlastp 4617switchgrass|12v1|FE614174_P1 7989 704 96.1 globlastp 4618switchgrass|gb167|FE614174 7990 704 95.6 globlastp 4619millet|10v1|EVO454PM015232_P1 7991 704 95 globlastp 4620cynodon|10v1|ES302192_P1 7992 704 91.7 globlastp 4621lovegrass|gb167|DN480722_T1 7993 704 91.67 glotblastn 4622brachypodium|12v1|BRADI4G03060_T1 7994 704 88.33 glotblastn 4623oat|11v1|GO582551_P1 7995 704 87.9 globlastp 4624 oat|11v1|GO582656_P17995 704 87.9 globlastp 4625 wheat|12v3|TAU22442 7996 704 87.9 globlastp4626 leymus|gb166|EG383427_P1 7997 704 87.4 globlastp 4627pseudoroegneria|gb167|FF342320 7998 704 87.4 globlastp 4628lolium|10v1|AU248479_P1 7999 704 86.8 globlastp 4629 rye|12v1|BE5878628000 704 86.8 globlastp 4630 eucalyptus|11v2|CT983413_P1 8001 704 84.2globlastp 4631 trigonella|11v1|SRR066194X122729 8002 704 84.2 globlastp4632 rice|11v1|BE039706 8003 704 83.96 glotblastn 4633euphorbial|11v1|SRR098678X164620_P1 8004 704 83.9 globlastp 4634chickpea|11v1|GR390844 8005 704 83.6 globlastp 4635chickpea|13v2|GR390844_P1 8005 704 83.6 globlastp 4636oil_palm|11v1|EL691105_P1 8006 704 83.6 globlastp 4637tomato|11v1|BG123164 8007 704 83.6 globlastp 4638fraxinus|11v1|SRR058827.164804_T1 8008 704 83.33 glothlastn 4639antirrhinum|gb166|AJ568560_P1 8009 704 83.3 globlastp 4640primula|11v1|SRR098679X101388_P1 8010 704 83.3 globlastp 4641medicago|12v1|AW127578_P1 8011 704 83.2 globlastp 4642curcuma|10v1|DY388752_P1 8012 704 83.1 globlastp 4643peanut|10v1|GO323872_P1 8013 704 83.1 globlastp 4644olea|13v1|SRR014463X35572D1_T1 8014 704 82.87 glothlastn 4645nicotiana_bentharniana|12v1|CN742663_P1 8015 704 82.2 globlastp 4646eggplant|10v1|FS000347_P1 8016 704 82.2 globlastp 4647lotus|09v1|B1418063_P1 8017 704 82.2 globlastp 4648phalaenopsis|11v1|SRR125771.1037360_P1 8018 704 82.1 globlastp 4649platanus|11v1|SRR096786X128388_P1 8019 704 82 globlastp 4650hornbeam|12v1|SRR364455.120478_T1 8020 704 81.72 glotblastn 4651blueberry|12v1|CF810435_P1 8021 704 81.7 globlastp 4652fraxinus|11v1|SRR058827.121081_T1 8022 704 81.67 glotblastn 4653orobanche|10v1|SRR023189S0019436_T1 8023 704 81.67 glotblastn 4654onion|12v1|SRR073446X110681D1_P1 8024 704 81.6 globlastp 4655tobacco|gb162|DV158342 8025 704 81.6 globlastp 4656flax|11v1|EU828929_P1 8026 704 81.5 globlastp 4657 flax|11v1|JG090232_P18027 704 81.5 globlastp 4658 ambrosia|11v1|SRR346943.137299_P1 8028 70481.4 globlastp 4659 curcurbita|11v1|FG227219XX_P1 8029 704 81.4globlastp 4660 phalaenopsis|11v1|SRR125771.1054625_T1 8030 704 81.32glotblastn 4661 bean|12v2|CA896575_T1 8031 704 81.18 glothlastn 4662bean|12v1|CA896575 8031 704 81.18 glotblastn 4663aquilegia|10v2|JGIAC006472_T1 8032 704 81.11 glotblastn 4664soybean|11v1|GLYMA06G04850 8033 704 81.11 glotblastn 4665soybean|12v1|GLYMA06G04850_T1 8033 704 81.11 glotblastn 4666tabernaemontana|11v1|SRR098689X124415 8034 704 81.11 glotblastn 4667tragopogon|10v1|SRR020205S0035974 8035 704 81.11 glotblastn 4668tobacco|gb162|CV021615 8036 704 81.1 globlastp 4669otea|13v1|SRR014463X30554D1_P1 8037 704 81.1 globlastp 4670pepper|12v1|BM059712_P1 8038 704 81 globlastp 4671amorphophallus|11v2|SRR089351X125225_T1 8039 704 80.98 glotblastn 4672grape|11v1|GSVIVT01009358001_P1 8040 704 80.9 globlastp 4673cleome_spinosa|10v1|GR931673_T1 8041 704 80.87 glotblastn 4674phalaenopsis|11v1|SRR125771.1037068XX1_T1 8030 704 80.77 glotblastn 4675poppy|11v1|FE964711_P1 8042 704 80.7 globlastp 4676cleome_spinosa|10v1|GR930987_P1 8043 704 80.6 globlastp 4677clover|gb162|BB929026_P1 8044 704 80.6 globlastp 4678ambrosia|11v1|SRR346935.316071_T1 8045 704 80.56 glotblastn 4679centaurea|gb166|EH717726 8046 704 80.56 glotblastn 4680cirsium|11v1|SRR346952.11256_T1 8047 704 80.56 glothlastn 4681cowpea|12v1|FF387663_T1 8048 704 80.56 glothlastn 4682ipomoea_batatas|10v1|DV034551_T1 8049 704 80.56 glotblastn 4683poppy|11v1|SRR030259.103371_T1 8050 704 80.56 glotbiastn 4684poppy|11v1|SRR030260.169771_T1 8051 704 80.56 glotblastn 4685thalictrum|11v1|SRR096787X108014 8052 704 80.56 glotblastn 4686thalictrum|11v1|SRR096787X11261 8053 704 80.56 glotblastn 4687vinca|11v1|SRR098690X110157 8054 704 80.56 glothlastn 4688petunia|gb171|CV301011_P1 8055 704 80.5 globlastp 4689solanum_phureja|09v1|SPHBG123164 8056 704 80.5 globlastp 4690soybean|11v1|GLYMA04G04770 8057 704 80.43 glotblastn 4691soybean|12v1|GLYMA04G04770_T1 8057 704 80.43 glotblastn 4692beech|11v1|SRR006293.20456_P1 8058 704 80.4 globlastp 4693poplar|10v1|AI163073 8059 704 80.4 globlastp 4694poplar|13v1|AI162099_P1 8059 704 80.4 globlastp 4695spruce|11v1|ES878106 8059 704 80.4 globlastp 4696cannabis|12v1|SOLX00055197_T1 8060 704 80.33 glotblastn 4697apple|11v1|CN444425_P1 8061 704 80.3 globlastp 4698cucurbita|11v1|SRR091276X112897_P1 8062 704 80.3 globlastp 4699euphorbia|11v1|BP958713_P1 8063 704 80.3 globlastp 4700sunflower|12v1|CD848609 8064 704 80.3 globlastp 4701sunflower|12v1|DY928392 8064 704 80.3 globlastp 4702pigeonpea|11v1|SRR054580X108129_T1 8065 704 80.11 glotblastn 4703valeriana|11v1|SRR099039X123814 8066 704 80.1 globlastp 4704nicotiana_benthamiana|12v1|DV161035_T1 8067 704 80 glotblastn 4705b_juncea|12v1|E6ANDIZ01BABOV_T1 8008 704 80 glotblastn 4706clementine|11v1|CB292000_P1 8069 704 80 globlastp 4707flaveria|11v1|SRR149229.140630_T1 8070 704 80 glotblastn 4708maritime_pine|10v1|BX250460_T1 8071 704 80 glotblastn 4709monkeyflower|10v1|DV210529 8072 704 80 glotblastn 4710monkeyflower|12v1|DV210529_T1 8072 704 80 glotblastn 4711olea|11v1|SRR014463.30554 8073 704 80 globlastp 4712orange|11v1|CB292000_P1 8069 704 80 globlastp 4713phyla|11v2|SRR099035X104250_T1 8074 704 80 glotblastn 4714pine|10v2|AW056696_T1 8071 704 80 glotblastn 4715potato|10v1|AJ487457_P1 8075 704 80 globlastp 4716triphysaria|10v1|EX988218 8076 704 80 globlastp 4717tripterygium|11v1|SRR098677X101252 8077 704 80 glotblastn 4718maize|10v1|BM500443_P1 8078 705 92.8 globlastp 4719sorghum|12v1|SB10G02193_P1 8079 705 91 globlastp 4720maizei|10v1|ZMCRP2V110874_P1 8080 707 98 globlastp 4721sorghum|12v1|SB07G004810 8081 707 94.08 glotblastn 4722rice|11v1|BI809598 8082 707 84.2 glotblastn 4723 rice|11v1|CB209742 8083707 83.76 glotblastn 4724 rice|11v1|AU057895 8084 707 82.2 glotblastn4725 foxtail_millet|11v3|PHY7S1009239M_T1 8085 707 81.79 glotblastn 4726brachypodium|12v1|BRADI5G23367_T1 8086 707 80.86 glothlastn 4727switchgrass|12v1|FE616344_T1 8087 707 80.59 glothlastn 4728maize|10v1|T23354_T1 8088 707 80.37 glotblastn 4729sorgbum|12v1|SB10G028730 8089 710 84.1 globlastp 4730soybean|11v1|GLYMA14G24660 8090 711 88.06 glotblastn 4731soybean|12v1|GLYMA14G24660T2_P1 8091 711 86.4 globlastp 4732brachypodium|12v1|BRADI4G29730_P1 8092 713 86.1 globlastp 4733barley|12v1|BE411017_T1 8093 713 83.12 glotblastn 4734switchgrass|gb167|DN145699 8094 713 80.1 globlastp 4735rice|11v1|BI306619 8095 713 80 globlastp 4736 wheat|12v3|BE417065_P18096 715 91.8 globlastp 4737 rye|12v1|DRR001012.157648_P1 8097 715 91.3globlastp 4738 wheat|12v3|AL823091 8098 719 95.2 globlastp 4739rye|12v1|DRR001012.107133 8099 719 95.14 glotblastn 4740brachypodium|12v1|BRADI3G37350_P1 8100 719 84.2 globlastp 4741rice|11v1|AU108306 8101 719 80 globlastp 4742 wheat|12v3|BE488612 8102721 92.7 globlastp 4743 rye|12v1|DRR001012.125660 8103 721 91.2globlastp 4744 pseudoroegneria|gb167|FF340927 8104 721 90.8 globlastp4745 wheat|12v3|CD863372 8105 725 96.4 globlastp 4746wheat|12v3|AK330727 8106 725 96.3 globlastp 4747wheat|12v3|SRR043326X28070D1 8107 725 95.93 glotblastn 4748wheat|12v3|CA639363 8108 725 94.6 globlastp 4749wheat|12v3|SRR073321X143332D1 8109 725 88.9 globlastp 4750wheat|12v3|CD863371 8110 725 85.06 glotblastn 4751 rice|11v1|CA9981458111 725 82.3 globlastp 4752 rye|12v1|DRR001012.117306 8112 727 96globlastp 4753 wheat|12v3|BM134597 8113 727 94.1 globlastp 4754brachypodium|12v1|BRADI3G50150_P1 8114 727 90.9 globlastp 4755wheat|12v3|AL826717 8115 727 84.6 glotblastn 4756 rice|11v1|CK0613798116 727 84.5 globlastp 4757 switchgrass|12v1|FE608601_P1 8117 727 83.6globlastp 4758 switchgrass|12v1|FE623318_P1 8118 727 83.6 globlastp 4759foxtail_millet|11v3|PHY7SI016281M_P1 8119 727 83.3 globlastp 4760maize|10v1|AW066736_P1 8120 727 82.2 globlastp 4761rye|12v1|DRR001013.102 8121 730 83.9 globlastp 4762switchgrass|gb167|FE639307 8122 733 87.8 globlastp 4763switchgrass|gb167|FL868802 8123 733 86.2 globlastp 4764sorghum|12v1|SB07G026630 8124 733 83 globlastp 4765millet|10v1|PMSLX0018649D1_T1 8125 735 80.11 glotblastn 4766switchgrass|12v1|FL802295_P1 8126 736 87.5 globlastp 4767switchgrass|12v1|FE638239_P1 8127 736 84.8 globlastp 4768sugarcane|10v1|CA133855_P1 8128 736 80.5 globlastp 4769maize|10v1|W49426_P1 8129 737 95.7 globlastp 4770switchgrass|gb167|FE649778 8130 737 85.9 globlastp 4771cenchrus|gb166|EB659527_P1 8131 738 84.9 globlastp 4772sorghum|12v1|SB06G024550 8132 740 94.6 globlastp 4773 rice|11v1|BE0404888133 740 87.5 globlastp 4774 rice|11v1|D39716 8134 740 81.8 globlastp4775 rice|11v1|BE230101 8135 740 81.3 glotblastn 4776barley|12v1|AJ432549_P1 8136 740 80.5 globlastp 4777sorghum|12v1|SB03G002350 8137 742 89.86 glotblastn 4778switchgrass|12v1|FL844034_T1 8138 742 84.29 glotblastn 4779switchgrass|gb167|FL844034 8139 742 84.29 glotblastn 4780switchgrass|12v1|FE603085_T1 8140 742 80.28 glotblastn 4781maize|10v1|BM500226_P1 8141 745 80.2 globlastp 4782switchgrass|12v1|FL754128_P1 8142 746 90.4 globlastp 4783barley|12v1|DN155880_P1 8143 746 80 globlastp 4784 rice|11v1|B18077768144 746 80 globlastp 4785 maize|10v1|BM501045_P1 8145 748 94 globlastp4786 sorghum|12v1|SB01G007850 8146 748 94 globlastp 4787millet|10v1|EVO454PM001162_P1 8147 748 91.3 globlastp 4788switchgrass|12v1|FL702906_P1 8148 748 89.5 globlastp 4789rice|11v1|GFXAF141942X1 8149 748 87.6 globlastp 4790sorghum|12v1|SB01G048260 8150 749 94.4 globlastp 4791maize|10v1|AI491652_P1 8151 749 93.7 globlastp 4792switchgrass|12v1|FE656148_P1 8152 749 87.4 globlastp 4793foxtail_millet|11v3|PHY7SI035085M_P1 8153 749 87.4 globlastp 4794switchgrass|12v1|FL696332_P1 8154 749 86.7 globlastp 4795switchgrass|gb167|FE656148 8155 749 85.66 glotblastn 4796rice|11v1|BI795865 8156 749 81.1 globlastp 4797brachypodium|12v1|BRADI1G76321_P1 8157 749 80.4 globlastp 4798brachypodium|12v1|BRADI1G76314_P1 8158 749 80 globlastp 4799foxtail_millet|11v3|PHY7SI013180M_P1 8159 750 84.2 globlastp 4800switchgrass|12v1|FL691835_P1 8160 750 83.3 globlastp 4801sugarcane|10v1|CA073362 8161 751 84.31 glotblastn 4802sorghum|12v1|SB02G024920 8162 751 84.06 glotblastn 4803maize|10v1|DR795538_T1 8163 751 83.5 glotblastn 4804foxtail_millet|11v3|PHY7SI029454M_P1 8164 754 80.7 globlastp 4805foxtail_millet|11v3|PHY7SI030299M_P1 8165 756 80.1 globlastp Providedare the homologous (e.g orthologues) polypeptides and polynucleotides ofthe genes for increasing yield (e.g., oil yield, seed yield, fiber yieldand/or quality), growth rate, vigor, photosynthetic capacity, biomass,abiotic stress tolerance, nitrogen use efficiency, water use efficiencyand fertilizer use efficiency genes of a plant which are listed in Table1 above. Homology was calculated as % of identity over the alignedsequences. The query sequences were polynucleotide sequences SEQ ID NOs:1-473; and polypeptide SEQ ID NOs: 474-760 and the subject sequences areprotein sequences identified in the database based on greater than 80%global identity to the predicted translated sequences of the querynucleotide sequences or to the polypeptide sequences. “P.N.” =polynucleotide; “P.P.” = polypeptide; “Algor.” = algorithm (used forsequence alignment and determination of percent homology);“Hom.”—homology; “iden.”—identity.

The output of the functional genomnics approach described herein is aset of genes highly predicted to improve yield and/or other agronomicimportant traits such as growth rate, harvest index, leaf area, vigor,oil content, fiber yield and/or quality, biomass, photosyntheticcapacity, growth rate, abiotic stress tolerance, nitrogen useefficiency, water use efficiency and fertilizer use efficiency of aplant by increasing their expression. Although each gene is predicted tohave its own impact, modifying the mode of expression of more than onegene is expected to provide an additive or synergistic effect on theplant yield and/or other agronomic important yields performance.Altering the expression of each gene described here alone or set ofgenes together increases the overall yield and/or other agronomicimportant traits, hence expects to increase agricultural productivity.

Example 3 Production of Barley Transcriptome and High ThroughputCorrelation Analysis Using 44K Barley Oligonucleotide Micro-Array

In order to produce a high throughput correlation analysis, the presentinventors utilized a Barley oligonucleotide micro-array, produced byAgilent Technologies [chem. (dot) agilent (dot) com/Scripts/PDS (dot)asp?1Page=50879]. The array oligonucleotide represents about 47,500Barley genes and transcripts. In order to define correlations betweenthe levels of RNA expression and yield or vigor related parameters,various plant characteristics of 25 different Barley accessions wereanalyzed. Among them, 13 accessions encompassing the observed variancewere selected for RNA expression analysis. The correlation between theRNA levels and the characterized parameters was analyzed using Pearsoncorrelation test [davidmlane (dot) com/hyperstat/A34739 (dot) html].

Experimental Procedures

Four tissues at different developmental stages [meristem, floweringspike, booting spike, stem], representing different plantcharacteristics, were sampled and RNA was extracted as describedhereinabove under “GENERAL EXPERIMENTAL AND BIOINFORMATICS METHODS”.

For convenience, each micro-array expression information tissue type hasreceived a Set ID as summarized in Table 3 below.

TABLE 3 Barley transcriptome expression sets Expression Set Set IDbooting spike at flowering stage 1 flowering spike at flowering stage 2Meristein at flowering stage 3 stem at flowering stage 4 Table 3:Provided are the identification (ID) letters of each of the Barleyexpression sets.

Barley yield components and vigor related parameters assessment—13Barley accessions in 4 repetitive blocks (named A, B, C, and D), eachcontaining 4 plants per plot were grown at net house. Plants werephenotyped on a daily basis following the standard descriptor of barley(Table 4, below). Harvest was conducted while 50% of the spikes were dryto avoid spontaneous release of the seeds. Plants were separated to thevegetative part and spikes, of them, 5 spikes were threshed (grains wereseparated from the glumes) for additional grain analysis such as sizemeasurement, grain count per spike and grain yield per spike. Allmaterial was oven dried and the seeds were threshed manually from thespikes prior to measurement of the seed characteristics (weight andsize) using scanning and image analysis. The image analysis systemincluded a personal desktop computer (Intel P4 3.0 GHz processor) and apublic domain program—ImageJ 1.37 (Java based image processing program,which was developed at the U.S. National Institutes of Health and freelyavailable on the internet [rsbweb (dot) nih (dot) gov/]. Next, analyzeddata was saved to text files and processed using the JMP statisticalanalysis software (SAS institute).

TABLE 4 Barley standard descriptors Trait Parameter Range DescriptionGrowth habit Scoring 1-9 Prostrate (1) or Erect (9) Hairiness of ScoringP (Presence)/A Absence (1) or basal leaves (Absence) Presence (2) StemScoring 1-5 Green (1), Basal only or pigmentation Half or more (5) Daysto Days Days from sowing to Flowering emergence of awns Plant heightCentimeter Height from ground level to (cm) top of the longest spikeexcluding awns Spikes per plant Number Terminal Counting Spike lengthCentimeter Terminal Counting (cm) 5 spikes per plant Grains per spikeNumber Terminal Counting 5 spikes per plant Vegetative dry GramOven-dried for 48 hours weight at 70° C. Spikes dry Gram Oven-dried for48 hours weight at 30° C. Table 4.

At the end of the experiment (50% of the spikes were dry) all spikesfrom plots within blocks A-D were collected, and the followingmeasurements were performed:

(i) Grains per spike—The total number of grains from 5 spikes that weremanually threshed was counted. The average grain per spike wascalculated by dividing the total grain number by the number of spikes.

(ii) Grain average size (cm)—The total grains from 5 spikes that weremanually shed were scanned and images were analyzed using the digitalimaging system. Grain scanning was done using Brother scanner (modelDCP-135), at the 200 dpi resolution and analyzed with Image J software.The average grain size was calculated by dividing the total grain sizeby the total grain number.

(iii) Grain average weight (mgr)—The total grains from 5 spikes thatwere manually threshed were counted and weight. The average weight wascalculated by dividing the total weight by the total grain number.

(iv) Grain yield per spike (gr) (=seed yield of 5 spikes)—The totalgrains from 5 spikes that were manually threshed were weight. The grainyield was calculated by dividing the total weight by the spike number.

(v) Spike length analysis—The five chosen spikes per plant were measuredusing measuring tape excluding the awns.

(vi) Spike number analysis—The spikes per plant were counted.

Additional parameters were measured as follows:

Growth habit scoring—At growth stage 10 (booting), each of the plantswas scored for its growth habit nature. The scale that was used was 1for prostate nature till 9 for erect.

Hairiness of basal leaves—At growth stage 5 (leaf sheath strongly erect;end of tillering), each of the plants was scored for its hairinessnature of the leaf before the last. The scale that was used was 1 forprostate nature till 9 for erect.

Plant height—At harvest stage (50% of spikes were dry), each of theplants was measured for its height using measuring tape. Height wasmeasured from ground level to top of the longest spike excluding awns.

Days to flowering—Each of the plants was monitored for flowering date.Days of flowering was calculated from sowing date till flowering date.

Stem pigmentation—At growth stage 10 (booting), each of the plants wasscored for its stem color. The scale that was used was 1 for green till5 for full purple.

Vegetative dry weight and spike yield—At the end of the experiment (50%of the spikes were dry) all spikes and vegetative material from plotswithin blocks A-D are collected. The biomass and spikes weight of eachplot was separated, measured and divided by the number of plants.

Dry weight=total weight of the vegetative portion above ground(excluding roots) after drying at 70° C. in oven for 48 hours;

Spike yield per plant=total spike weight per plant (gr) after drying at30° C. in oven for 48 hours.

TABLE 5 Barley correlated parameters (rectors) Correlated parameter withCorrelation ID Grain weight (milligrams) 1 Grains size (mm²) 2 Grainsper spike (numbers) 3 Growth habit (scores 1-9) 4 Hairiness of basalleaves (scoring 1-2) 5 Plant height (cm) 6 Seed yield of 5 spikes (gr) 7Spike length (cm) 8 Spikes per plant (numbers) 9 Stem pigmentation(scoring 1-5) 10 Vegetative dry weight (gram) 11 Days to flowering(days) 12 Table 5. Provided are the Barley correlated parameters(vectors).

Experimental Results

13 different Barley accessions were grown and characterized for 12parameters as described above. The average for each of the measuredparameter was calculated using the JMP software and values aresummarized in Tables 6and 7below. Subsequent correlation analysisbetween the various transcriptome expression sets (Table 3) and theaverage parameters was conducted. Follow, results were integrated to thedatabase (Table 8below).

TABLE 6 Measured parameters of correlation IDs in Barley accessions Eco-type/ Treat- ment Line-1 Line-2 Line-3 Line-4 Line-5 Line-6 Line-7  135.05 28.06 28.76 17.87 41.22 29.73 25.22  2 0.27 0.23 0.24 0.17 0.290.28 0.22  3 20.23 17.98 17.27 17.73 14.47 16.78 12.12  4 2.60 2.00 1.923.17 4.33 2.69 3.60  5 1.53 1.33 1.69 1.08 1.42 1.69 1.30  6 134.27130.50 138.77 114.58 127.75 129.38 103.89  7 3.56 2.54 2.58 1.57 3.032.52 1.55  8 12.04 10.93 11.83 9.90 11.68 11.53 8.86  9 48.85 48.2737.42 61.92 33.27 41.69 40.00 10 1.13 2.50 1.69 1.75 2.33 2.31 1.70 1178.87 66.14 68.49 53.39 68.30 74.17 35.35 12 62.40 64.08 65.15 58.9263.00 70.54 52.80 Table 6. Provided are the values of each of theparameters measured in Barley accessions according to the correlationidentifications (see Table 5).

TABLE 7 Barley accessions, additional measured parameters Eco- type/Treat- ment Line-8 Line-9 Line-10 Line-11 Line-12 Line-13  1 34.99 20.5827.50 37.13 29.56 19.58  2 0.28 0.19 0.22 0.27 0.27 0.18  3 14.07 21.5412.10 13.40 15.28 17.07  4 3.50 3.00 3.67 2.47 3.50 3.00  5 1.19 1.001.17 1.60 1.08 1.17  6 121.63 126.80 99.83 121.40 118.42 117.17  7 2.622.30 1.68 2.68 2.35 1.67  8 11.22 11.11 8.58 10.18 10.51 9.80  9 40.6362.00 49.33 50.60 43.09 51.40 10 2.19 2.30 1.83 3.07 1.58 2.17 11 58.3362.23 38.32 68.31 56.15 42.68 12 60.88 58.10 53.00 60.40 64.58 56.00Table 7. Provided are the values of each of the parameters measured inBarley accessions according to the correlation identifications (seeTable 5).

TABLE 8 Correlation between the expression level 4 the selectedpolynucleotides of the invention and their homologues in specifictissues or develop- mental stages and the phenotypic performance acrossBarley accessions Corr. Corr. Gene Exp. Set Gene Exp. Set Name R P valueset ID Name R P value set ID LYM1018 0.71 1.44E−02 3 9 LYM1018 0.865.95E−04 3 3 LYM1018 0.79 3.73E−03 3 12  LYM1019 0.73 1.01E−02 1 2LYM1019 0.71 1.48E−02 1 1 LYM1020 0.82 2.04E−03 3 9 LYM1024 0.731.58E−02 2 2 LYM1026 0.76 6.97E−03 3 10  LYM1027 0.83 1.63E−03 3 9LYM1029 0.70 1.62E−02 1 2 LYM1029 0.71 1.37E−02 1 1 LYM1029 0.701.60E−02 1 7 LYM1029 0.72 1.31E−02 3 7 LYM1029 0.88 3.86E−04 3 11 LYM1030 0.79 3.61E−03 1 2 LYM1030 0.77 5.34E−03 1 1 LYM1030 0.749.84E−03 1 7 LYM1040 0.86 6.47E−04 1 1 LYM1040 0.85 8.84E−04 1 2 LYM10400.73 1.09E−02 1 5 LYM1040 0.84 1.09E−03 1 7 LYM1060 0.79 3.94E−03 1 6LYM1040 0.76 6.56E−03 3 9 LYM1060 0.76 6.99E−03 1 7 LYM1051 0.721.94E−02 2 4 LYM1060 0.73 1.14E−02 1 3 LYM1060 0.78 4.36E−03 1 8 LYM10620.70 1.56E−02 3 9 LYM1060 0.74 9.49E−03 1 11  LYM1071 0.74 9.11E−03 1 4LYM1060 0.78 4.38E−03 3 9 LYM1072 0.71 1.54E−02 1 9 LYM1074 0.711.52E−02 3 9 LYM1074 0.73 1.02E−02 1 9 LYM1071 0.83 1.65E−03 3 9 LYM10750.85 1.03E−03 3 9 LYM1072 0.83 1.39E−03 3 9 Table 8. Provided are thecorrelations (R) and p-values (P) between the expression levels ofselected genes of some embodiments of the invention in various tissuesor developmental stages (Expression sets) and the phenotypic performancein various yield (seed yield, oil yield, oil content), biomass, growthrate and/or vigor components [Correlation (Corr.) vector (Vec.)Expression (Exp.)] Corr. Vector = correlation vector specified in Tables5, 6 and 7; Exp. Set = expression set specified in Table 3.

Example 4 Production of Sorghum Transcriptome and High ThroughputCorrelation Analysis with ABST Related Parameters Using 44K SorghumOligonucleotide Micro-Arrays

In order to produce a high throughput correlation analysis between plantphenotype and gene expression level, the present inventors utilized asorghum oligonucleotide micro-array, produced by Agilent Technologies[chem. (dot) agilent (dot) com/Scripts/PDS (dot) asp?1Page=50879]. Thearray oligonucleotide represents about 44.000 sorghum genes andtranscripts. In order to define correlations between the levels of RNAexpression with ABST, yield and NUE components or vigor relatedparameters, various plant characteristics of 17 different sorghumhybrids were analyzed. Among them, 10 hybrids encompassing the observedvariance were selected for RNA expression analysis. The correlationbetween the RNA levels and the characterized parameters was analyzedusing Pearson correlation test [davidmlane (dot) com/hyperstat/A34739(dot) html].

I. Correlation of Sorghum Varieties Across Ecotypes Grown Under RegularGrowth Conditions, Severe Drought Conditions and Low Nitrogen Conditions

Experimental Procedures

17 Sorghum varieties were grown in 3 repetitive plots, in field.Briefly, the growing protocol was as follows:

1. Regular (normal, non-stress) growth conditions: sorghum plants weregrown in the field using commercial fertilization and irrigationprotocols (0.370 liter per meter², fertilization of 14 units of 21% ureaper entire growth period).

2. Drought conditions: sorghum seeds were sown in soil and grown undernormal (non-stress) conditions until around 35 days from sowing, aroundstage V8 (eight green leaves are fully expanded, booting not startedyet). At this point, irrigation was stopped, and severe drought stresswas developed.

3. Low Nitrogen fertilization conditions: sorghum plants were fertilizedwith 50% less amount of nitrogen in the field than the amount ofnitrogen applied in the regular growth treatment. All the fertilizer wasapplied before flowering.

Analyzed Sorghum tissues—All 10 selected Sorghum hybrids were sampledper each treatment. Tissues [Flag leaf. Flower meristem and Flower] fromplants growing under normal conditions, severe drought stress and lownitrogen conditions were sampled and RNA was extracted as describedabove. Each micro-array expression information tissue type has receiveda Set ID as summarized in Table 9 below.

TABLE 9 Sorgbum transeriptome expression sets Expression Set Set ID Flagleaf at flowering stage under drought growth 1 conditions Flag leaf atflowering stage under low nitrogen growth 2 conditions Flag leaf atflowering stage under normal growth 3 conditions Flower meristem atflowering stage under drought growth 4 conditions Flower meristem atflowering stage under low nitrogen 5 growth conditions Flower meristemat flowering stage under normal growth 6 conditions Flower at floweringstage under drought growth 7 conditions Flower at flowering stage underlow nitrogen growth 8 conditions Flower at flowering stage under normalgrowth conditions 9 Table 9: Provided are the sorghum transcriptomeexpression sets 1, 2, 3 and 4. Flag leaf = the leaf below the flower;Flower meristem = Apical meristem following panicle initiation; Flower =the flower at the anthesis day. Expression sets 1, 2 and 3 are fromplants grown under normal conditions. Expression set 4 derived fromplants grown under drought conditions.The following parameters were collected using digital imaging system:

At the end of the growing period the grains were separated from theplant ‘Head’ and the following parameters were measured and collected:

Average Grain Area (cm²)—A sample of ˜200 grains were weighted,photographed and images were processed using the below described imageprocessing system. The grain area was measured from those images and wasdivided by the number of grains.

Upper and Lower Ratio Average of Grain Area, width, diameter andperimeter—Grain projection of area, width, diameter and perimeter wereextracted from the digital images using open source package imagej(nih). Seed data was analyzed in plot average levels as follows:

Average of all seeds;

Average of upper 20% fraction—contained upper 20% fraction of seeds;

Average of lower 20% fraction—contained lower 20% fraction of seeds;

Further on, ratio between each fraction and the plot average wascalculated for each of the data parameters.

At the end of the growing period 5 ‘Heads’ were, photographed and imageswere processed using the below described image processing system.

(i) Head Average Area (cm²)—At the end of the growing period 5 ‘Heads’were, photographed and images were processed using the below describedimage processing system. The ‘Head’ area was measured from those imagesand was divided by the number of ‘Heads’.

(ii) Head Average Length (cm)—At the end of the growing period 5 ‘Heads’were, photographed and images were processed using the below describedimage processing system. The ‘Head’ length (longest axis) was measuredfrom those images and was divided by the number of ‘Heads’.

(i) Head Average width (cm)—At the end of the growing period 5 ‘Heads’were, photographed and images were processed using the below describedimage processing system. The ‘Head’ width was measured from those imagesand was divided by the number of ‘Heads’.

(iv) Head Average perimeter (cm)—At the end of the growing period 5‘Heads’ were, photographed and images were processed using the belowdescribed image processing system. The ‘Head’ perimeter was measuredfrom those images and was divided by the number of ‘Heads’.

The image processing system was used, which consists of a personaldesktop computer (Intel P4 3.0 GHz processor) and a public domainprogram—ImageJ 1.37, Java based image processing software, which wasdeveloped at the U.S. National Institutes of Health and is freelyavailable on the internet at rsbweb (dot) nih (dot) gov/. Images werecaptured in resolution of 10 Mega Pixels (3888×2592 pixels) and storedin a low compression JPEG (Joint Photographic Experts Group standard)format. Next, image processing output data for seed area and seed lengthwas saved to text files and analyzed using the JMP statistical analysissoftware (SAS institute).

Additional parameters were collected either by sampling 5 plants perplot or by measuring the parameter across all the plants within theplot.

Total Grain Weight/Head (gr.) (grain yield)—At the end of the experiment(plant ‘Heads’) heads from plots within blocks A-C were collected. 5heads were separately threshed and grains were weighted, all additionalheads were threshed together and weighted as well. The average grainweight per head was calculated by dividing the total grain weight bynumber of total heads per plot (based on plot). In case of 5 heads, thetotal grains weight of 5 heads was divided by 5.

FW Head/Plant gram—At the end of the experiment (when heads wereharvested) total and 5 selected heads per plots within blocks A-C werecollected separately. The heads (total and 5) were weighted (gr.)separately and the average fresh weight per plant was calculated fortotal (FW Head/Plant gr, based on plot) and for 5 (FW Head/Plant gr,based on 5 plants).

Plant height—Plants were characterized for height during growing periodat 5 time points. In each measure, plants were measured for their heightusing a measuring tape. Height was measured from ground level to top ofthe longest leaf.

SPAD—Chlorophyll content was determined using a Minolta SPAD 502chlorophyll meter and measurement was performed 64 days post sowing.SPAD meter readings were done on young fully developed leaf. Threemeasurements per leaf were taken per plot.

Vegetative fresh weight and Heads—At the end of the experiment (whenInflorescence were dry) all Inflorescence and vegetative material fromplots within blocks A-C were collected. The biomass and Heads weight ofeach plot was separated, measured and divided by the number of Heads.

Plant biomass (Fresh weight)—At the end of the experiment (whenInflorescence were dry) the vegetative material from plots within blocksA-C were collected. The plants biomass without the Inflorescence weremeasured and divided by the number of Plants.

FW Heads/(FW Heads+FW Plants)—The total fresh weight of heads and theirrespective plant biomass were measured at the harvest day. The headsweight was divided by the sum of weights of heads and plants.

Experimental Results

17 different sorghum varieties were grown and characterized fordifferent parameters: The average for each of the measured parameter wascalculated using the JMP software (Tables 11-12) and subsequentcorrelation analysis between the various transcriptome sets (Table 9)and the average parameters, was conducted (Table 13). Results were thenintegrated to the database.

TABLE 10 Sorgbum correlated parameters (vectors) Correlated parameterwith Correlation ID Average Grain Area (cm²), Drought 1 Average GrainArea (cm²), Low N 2 Average Grain Area (cm²) Normal 3 FW - Head/Plant gr(based on plot), Drought 4 FW - Head/Plant gr (based on plot), Low N 5FW - Head/Plant gr (based on plot), Normal 6 FW - Head/Plant gr (basedon 5 plants), Low N 7 FW - Head/Plant gr based on 5 plants), Normal 8 FWHeads/(FW Heads + FW Plants) (all plot), Drought 9 FW Heads/(FW Heads +FW Plants) (all plot), Low N 10 FW Heads/(FW Heads + FW Plants (allplot), Normal 11 FW/Plant gr (based on plot), Drought 12 FW/Plant gr(based on plot), Low N 13 FW/Plant gr (based on plot), Normal 14 FinalPlant Height (cm), Drought 15 Final Plant Height (cm), Low N 16 FinalPlant Height (cm), Normal 17 Head Average Area (cm²), Drought 18 HeadAverage Area (cm²), Low N 19 Head Average Area (cm²), Normal 20 HeadAverage Length (cm), Drought 21 Head Average Length (cm), Low N 22 HeadAverage Length (cm) Normal 23 Head Average Perimeter (cm), Drought 24Head Average Perimeter (cm) Low N 25 Head Average Perimeter (cm), Normal26 Head Average Width (cm), Drought 27 Head Average Width (cm), Low N 28Head Average Width (cm), Normal 29 Leaf SPAD 64 DPS (Days Post Sowing),Drought 30 Leaf SPAD 64 DPS (Days Post Sowing), Low N 31 Leaf SPAD 64DPS (Days Post Sowing), Normal 32 Lower Ratio Average Grain Area, Low N33 Lower Ratio Average Grain Area, Normal 34 Lower Ratio Average GrainLength, Low N 35 Lower Ratio Average Grain Length, Normal 36 Lower RatioAverage Grain Perimeter, Low N 37 Lower Ratio Average Grain Perimeter,Normal 38 Lower Ratio Average Grain Width Low N 39 Lower Ratio AverageGrain Width, Normal 40 Total grain weight/Head (based on plot) gr, Low N41 Total grain weight/Head gr (based on 5 heads), Low N 42 Total grainweight/Head gr (based on 5 heads), Normal 43 Total grain weight/Head gr(based on plot), Normal 44 Total grain weight/Head gr (based on plot)Drought 45 Upper Ratio Average Grain Area, Drought 46 Upper RatioAverage Grain Area, Low N 47 Upper Ratio Average Grain Area, Normal 48[Grain Yield + plant biomass/SPAD 64 DPS], Normal 49 [Grain Yield +plant biomass/SPAD 64 DPS], Low N 50 [Grain yield/SPAD 64 DPS], Low N 51[Grain yield/SPAD 64 DPS], Normal 52 [Plant biomass (FW)/SPAD 64 DPS],Drought 53 [Plant biomass (FW)/SPAD 64 DPS], Low N 54 [Plant biomass(FW)/SPAD 64 DPS], Normal 55 Table 10. Provided are the Sorghumcorrelated parameters (vectors). “gr.” = grams; “SPAD” = chlorophylllevels; “FW” = Plant Fresh weight; “normal” = standard growthconditions; “low N” = low nitrogen growth conditions; “drought” =drought growth conditions;

TABLE 11 Measured parameters in Sorghum accessions Eco- type/ Treat-Line- Line- Line- Line- Line- Line- Line- Line- Line- ment 1 2 3 4 5 6 78 9  1 0.099 0.115 0.106 0.094 0.090 0.114  2 0.105 0.111 0.136 0.1210.141 0.134 0.119 0.117 0.116  3 0.105 0.112 0.131 0.129 0.139 0.1410.110 0.113 0.102  4 154.90 122.02 130.51 241.11 69.03 186.41 62.1139.02 58.94  5 214.78 205.05 73.49 122.96 153.07 93.23 134.11 77.43129.63  6 175.15 223.49 56.40 111.62 67.34 66.90 126.18 107.74 123.86  7388.00 428.67 297.67 280.00 208.33 303.67 436.00 376.33 474.67  8 406.50518.00 148.00 423.00 92.00 101.33 423.50 386.50 409.50  9 0.42 0.47 0.420.37 0.23 0.31 0.41 0.44 0.40 10 0.505 0.506 0.166 0.391 0.210 0.1920.476 0.375 0.420 11 0.51 0.51 0.12 0.26 0.12 0.18 0.46 0.43 0.42 12207.99 138.02 255.41 402.22 233.55 391.75 89.31 50.61 87.02 13 204.78199.64 340.51 240.60 537.78 359.40 149.20 129.06 178.71 14 162.56 212.59334.83 313.46 462.28 318.26 151.13 137.60 167.98 15 89.40 75.73 92.1094.30 150.80 110.73 99.20 84.00 99.00 16 104.00 80.93 204.73 125.40225.40 208.07 121.40 100.27 121.13 17 95.25 79.20 197.85 234.20 189.40194.67 117.25 92. 80 112.65 18 83.14 107.79 88.68 135.91 90.76 123.9586.06 85.20 113.10 19 96.24 214.72 98.59 182.83 119.64 110.19 172.3684.81 156.25 20 120.14 167.60 85.14 157.26 104.00 102.48 168.54 109.32135.13 21 21.63 21.94 21.57 22.01 20.99 28.60 21.35 20.81 24.68 22 23.2225.58 20.93 28.43 24.32 22.63 32.11 20.38 26.69 23 25.58 26.84 21.0226.84 23.14 21.82 31.33 23.18 25.70 24 52.78 64.49 56.59 64.37 53.2171.66 55.61 52.96 69.83 25 56.32 79.20 53.25 76.21 67.27 59.49 79.2851.52 69.88 26 61.22 67.90 56.26 65.38 67.46 67.46 74.35 56.16 61.64 274.83 6.31 5.16 7.78 5.28 5.49 5.04 5.07 5.77 28 5.26 10.41 5.93 8.256.19 6.12 6.80 5.25 7.52 29 5.97 7.92 4.87 7.43 5.58 5.88 6.78 5.99 6.6230 40.58 40.88 45.01 42.30 45.24 40.56 44.80 45.07 40.65 31 38.33 38.9842.33 40.90 43.15 39.85 42.68 43.31 39.01 32 43.01 . 43.26 44.74 45.7641.61 45.21 45.14 43.03 33 0.815 0.770 0.810 0.793 0.780 0.799 0.8340.788 0.806 34 0.825 0.740 0.778 0.802 0.697 0.699 0.827 0.805 0.841 350.910 0.900 0.921 0.898 0.908 0.926 0.918 0.890 0.901 36 0.914 0.8840.921 0.908 0.890 0.877 0.913 0.903 0.920 37 0.901 0.884 0.915 0.8970.919 0.918 0.916 0.891 0.898 38 0.914 0.869 0.913 0.948 0.902 0.9150.913 0.910 0.918 39 0.901 0.852 0.893 0.880 0.863 0.871 0.910 0.8880.899 40 0.908 0.833 0.850 0.874 0.788 0.799 0.904 0.893 0.915 41 25.9530.57 19.37 35.62 25.18 22.18 49.96 27.48 51.12 42 50.27 50.93 36.1373.10 37.87 36.40 71.67 35.00 76.73 43 47.40 46.30 28.37 70.40 32.1549.23 63.45 44.45 56.65 44 31.12 26.35 18.72 38.38 26.67 28.84 47.6731.00 39.99 45 22.11 16.77 9.19 104.44 3.24 22.00 9.97 18.58 29.27 461.31 1.19 1.29 1.46 1.21 1.21 47 1.18 1.31 1.11 1.21 1.19 1.18 1.16 1.231.17 48 1.22 1.30 1.13 1.14 1.16 1.15 1.19 1.23 1.25 49 4.50 8.17 7.8710.68 8.34 4.40 3.74 4.83 3.67 50 6.02 5.91 8.50 6.75 13.05 9.58 4.673.61 5.89 51 0.68 0.78 0.46 0.87 0.58 0.56 1.17 0.63 1.31 52 3.78 7.747.01 10.10 7.65 3.34 3.05 3.90 2.83 53 5.13 3.38 5.67 9.51 5.16 9.661.99 1.12 2.14 54 5.34 5.12 8.05 5.88 12.46 9.02 3.50 2.98 4.58 55 0.720.43 0.86 0.58 0.69 1.05 0.69 0.93 0.84 Table 11: Provided are thevalues of each of the parameters (as described above) measured inSorghum accessions (ecotype) under normal, low nitrogen and droughtconditions. Growth conditions are specified in the experimentalprocedure section.

TABLE 12 Additional measured parameters in Sorghum accessions Ecotype/Line- Line- Line- Line- Line- Line- Line- Line- Treatment 10 11 12 13 1415 16 17  2 0.129 0.131 0.120 0.116 0.115 0.107 0.121 0.109  3 0.1180.121 0.111 0.117 0.108 0.105 0.110 0.105  4 76.37 33.47 42.20 41.53131.67 60.84 44.33 185.44  5 99.83 76.95 84.25 92.24 138.83 113.32 95.50129.49  6 102.75 82.33 77.59 91.17 150.44 109.10 107.58 130.88  7 437.67383.00 375.00 425.00 434.00 408.67 378.50 432.00  8 328.95 391.00 435.75429.50 441.00 415.75 429.50 428.50  9 0.443 0.472 0.468 0.484 0.3540.349 0.231 0.327 10 0.441 0.429 0.387 0.438 0.439 0.442 0.430 0.417 110.44 0.46 0.45 0.45 0.51 0.46 0.44 0.39 12 120.43 37.21 48.18 44.20231.60 116.01 123.08 342.50 13 124.27 101.33 132.12 117.90 176.99 143.67126.98 180.45 14 128.97 97.62 99.32 112.24 157.42 130.55 135.66 209.2115 92.20 81.93 98.80 86.47 99.60 83.00 83.53 92.30 16 94.53 110.00115.07 104.73 173.67 115.60 138.80 144.40 17 97.50 98.00 100.00 105.60151.15 117.10 124.45 126.50 18 100.79 80.41 126.89 86.41 92.29 77.8976.93 19 136.71 137.70 96.54 158.19 163.95 138.39 135.46 165.64 20169.03 156.10 112.14 154.74 171.70 168.51 162.51 170.46 21 24.28 21.9524.98 19.49 20.42 16.81 18.88 22 26.31 25.43 23.11 27.87 28.88 27.6425.52 30.33 23 28.82 28.13 22.97 28.09 30.00 30.54 27.17 29.26 24 65.1455.27 69.06 53.32 56.29 49.12 51.88 25 66.17 67.37 57.90 70.61 73.7666.87 65.40 75.97 26 71.40 68.56 56.44 67.79 71.54 78.94 67.03 74.11 275.37 4.66 6.35 5.58 5.76 5.86 5.10 28 6.59 6.85 5.32 7.25 7.19 6.27 6.576.82 29 7.42 6.98 6.19 7.02 7.18 7.00 7.39 7.35 30 45.43 42.58 44.1844.60 42.41 43.25 40.30 40.75 31 42.71 40.08 43.98 45.44 44.75 42.5843.81 46.73 32 45.59 44.83 45.33 46.54 43.99 45.09 45.14 43.13 33 0.770.741 0.804 0.788 0.823 0.801 0.809 0.807 34 0.79 0.765 0.803 0.8060.821 0.814 0.818 0.817 35 0.91 0.886 0.897 0.894 0.911 0.888 0.8920.901 36 0.92 0.893 0.913 0.907 0.911 0.904 0.903 0.913 37 0.91 0.8950.903 0.896 0.914 0.894 0.896 0.897 38 0.93 0.911 0.916 0.904 0.9120.905 0.909 0.905 39 0.86 0.842 0.897 0.887 0.908 0.899 0.902 0.897 400.85 0.863 0.885 0.898 0.905 0.910 0.902 0.899 41 36.84 29.45 26.7029.42 51.12 37.04 39.85 41.78 42 57.58 42.93 36.47 68.60 71.80 49.2743.87 52.07 43 60.00 45.45 58.19 70.60 70.10 53.95 59.87 52.65 44 38.3632.10 32.69 32.79 51.53 35.71 38.31 42.44 45 10.45 14.77 12.86 18.2411.60 18.65 16.36 47 1.22 1.24 1.19 1.23 1.16 1.34 1.21 1.21 48 1.241.32 1.22 1.18 1.18 1.22 1.25 1.22 49 2.89 2.91 3.12 4.75 3.69 3.85 5.8450 3.77 3.26 3.61 3.24 5.10 4.25 3.81 4.76 51 0.86 0.73 0.61 0.65 1.140.87 0.91 0.89 52 2.18 2.19 2.41 3.58 2.90 3.01 4.85 53 2.65 0.87 1.090.99 5.46 2.68 3.05 8.40 54 2.91 2.53 3.00 2.60 3.96 3.38 2.90 3.86 550.72 0.72 0.70 1 .17 0.79 0.85 0.98 Table 12: Provided are the values ofeach of the parameters as described above) measured in Sorghumaccessions (ecotype) under normal, low nitrogen and drought conditions.Growth conditions are specified in the experimental procedure section.

TABLE 13 Correlation between the expression level of selected genes ofsome embodiments of the invention in various tissues and the phenotypicperformance under normal or abiotic stress conditions across Sorghumaccessions Gene Exp. Corr. Gene Exp. Corr. Name R P value set Set IDName R P value set Set ID LYM1195 0.81 8.27E−03 9 55 LYM1195 0.741.41E−02 5 2 LYM1196 0.78 7.18E−03 6 3 LYM1196 0.82 3.74E−03 5 2 LYM12010.72 1.84E−02 6 40 LYM1201 0.83 2.97E−03 6 36 LYM1201 0.77 8.83E−03 6 34LYM1201 0.96 1.10E−05 5 41 LYM1201 0.95 3.28E−05 5 51 LYM1201 0.712.07E−02 5 16 LYM1201 0.76 1.71E−02 3 55 LYM1201 0.74 1.49E−02 1 53LYM1201 0.74 1.51E−02 1 12 LYM1202 0.73 1.57E−02 5 2 LYM1204 0.823.49E−03 6 6 LYM1204 0.85 1.65E−03 6 14 LYM1204 0.89 6.05E−04 3 6LYM1204 0.91 2.44E−04 3 14 LYM1205 0.80 5.08E−03 6 52 LYM1205 0.833.05E−03 6 49 LYM1205 0.79 6.92E−03 6 8 LYM1205 0.71 2.25E−02 8 5LYM1205 0.75 1.32E−02 8 50 LYM1205 0.84 2.13E−03 8 35 LYM1205 0.731.57E−02 3 3 LYM1205 0.73 2.41E−02 7 27 LYM1205 0.75 1.92E−02 7 24LYM1206 0.72 1.99E−02 2 47 LYM1206 0.74 1.52E−02 8 35 LYM1206 0.712.16E−02 8 37 LYM1206 0.91 5.65E−04 3 52 LYM1206 0.79 6.87E−03 3 6LYM1206 0.89 1.48E−03 3 49 LYM1206 0.71 2.27E−02 3 8 LYM1207 0.712.22E−02 6 44 LYM1207 0.70 2.37E−02 2 47 LYM1207 0.83 3.18E−03 5 5LYM1207 0.73 1.67E−02 5 7 LYM1207 0.86 1.54E−03 5 50 LYM1207 0.787.25E−03 5 54 LYM1207 0.79 6.59E−03 5 10 LYM1207 0.76 1.09E−02 5 13LYM1208 0.80 5.70E−03 9 3 LYM1208 0.76 1.16E−02 2 47 LYM1208 0.761.65E−02 3 52 LYM1208 0.80 9.89E−03 3 49 LYM1209 0.87 1.20E−03 6 3LYM1209 0.71 2.03E−02 2 47 LYM1209 0.85 1.83E−03 8 35 LYM1209 0.833.13E−03 8 37 LYM1209 0.77 9.60E−03 5 2 LYM1210 0.89 5.18E−04 6 3LYM1211 0.74 1.48E−02 6 3 LYM1211 0.72 1.91E−02 2 41 LYM1211 0.842.59E−03 2 16 LYM1211 0.81 4.89E−03 4 30 LYM1211 0.70 2.34E−02 5 2LYM1211 0.87 2.33E−03 1 18 LYM1211 0.77 1.44E−02 1 27 LYM1211 0.901.01E−03 1 24 LYM1211 0.71 3.24E−02 1 21 LYM1212 0.75 1.29E−02 6 3LYM1212 0.79 1.14E−02 3 52 LYM1212 0.84 4.17E−03 3 49 LYM1212 0.751.31E−02 1 30 LYM1213 0.72 1.94E−02 3 8 LYM1213 0.74 1.48E−02 1 4LYM1214 0.73 1.71E−02 6 40 LYM1214 0.72 1.77E−02 6 34 LYM1214 0.702.29E−02 2 47 LYM1214 0.72 1.99E−02 4 53 LYM1214 0.71 2.10E−02 4 12LYM1214 0.76 1.13E−02 5 33 LYM1239 0.71 2.13E−02 9 17 LYM1239 0.788.44E−03 9 44 LYM1239 0.90 4.03E−04 2 16 LYM1239 0.80 5.13E−03 4 30LYM1239 0.88 8.34E−04 8 37 LYM1239 0.71 2.08E−02 3 44 Table 13. Providedare the correlations (R) between the expression levels of yieldimproving genes and their homologues in tissues [Flag leaf, Flowermeristem, stem and Flower; Expression sets (Exp)] and the phenotypicperformance in various yield, biomass, growth rate and/or vigorcomponents [Correlation vector (corr.)] under stress conditions ornormal conditions across Sorghum accessions. P = p value.

II. Correlation of Sorghum Varieties Across Ecotype Grown Under SalinityStress and Cold Stress Conditions

Sorghum vigor related parameters under 100 mM NaCl and low temperature(10±2° C.)—Ten Sorghum varieties were grown in 3 repetitive plots, eachcontaining 17 plants, at a net house under semi-hydroponics conditions.Briefly, the growing protocol was as follows: Sorghum seeds were sown intrays filled with a mix of vermiculite and peat in a 1:1 ratio.Following germination, the trays were transferred to the high salinitysolution (100 mM NaCl in addition to the Full Hogland solution), lowtemperature (10±2° C. in the presence of Full Hogland solution) or atNormal growth solution [Full Hogland solution at 28±2° C.].

Full Hogland solution consists of: KNO₃-0.808 grams/liter. MgSO₄—0.12grams/liter, KH₂PO₄—0.172 grams/liter and 0.01% (volume/volume) of‘Super coratin’ micro elements (Iron-EDDHA[ethylenediamine-N,N′-bis(2-hydroxyphenylacetic 20 acid)]—40.5grams/liter; Mn—20.2 grams/liter; Zn 10.1 grams/liter; Co 1.5grams/liter; and Mo 1.1 grams/liter), solution's pH should be 6.5-6.8].

All 10 selected Sorghum varieties were sampled per each treatment. Twotissues [leaves and roots] growing at 100 mM NaCl, low temperature(10±2° C.) or under Normal conditions (full Hogland at a temperaturebetween 28±2° C.) were sampled and RNA was extracted as describedhereinabove under “GENERAL EXPERIMENTAL AND BIOINFORMATICS METHODS”.

TABLE 14 Sorghum transcriptome expression sets Expression Set Set IDroot at vegetative stage (V4-V5) under cold conditions 1 root vegetativestage (V4-V5) under normal conditions 2 root vegetative stage (V4-V5)under low nitrogen conditions 3 root vegetative stage (V4-V5) undersalinity conditions 4 vegetative meristem at vegetative stage (V4-V5)under 5 cold conditions vegetative meristem at vegetative stage (V4-V5)under low 6 nitrogen conditions vegetative meristem at vegetative stage(V4-V5) under 7 salinity conditions vegetative meristem at vegetativestage (V4-V5) under 8 normal conditions Table 14: Provided are theSorghum transcriptome expression sets. Cold conditions = 10 ± 2° C.;NaCl = 100 mM NaCl; low nitrogen = 1.2 mM Nitrogen; Normal conditions =16 mM Nitrogen.

Experimental Results

10 different Sorghum varieties were grown and characterized for thefollowing parameters: “Leaf number Normal”=leaf number per plant undernormal conditions (average of five plants); “Plant Height Normal”=plantheight under normal conditions (average of five plants); “Root DW 100 mMNaCl”—root dry weight per plant under salinity conditions (average offive plants); The average for each of the measured parameter wascalculated using the JMP software and values are summarized in Table 16below. Subsequent correlation analysis between the various transcriptomesets and the average parameters were conducted (Table 17). Results werethen integrated to the database.

TABLE 15 Sorghum correlated parameters (vectors) Corre- lationCorrelated parameter with ID DW Root/Plant − 100 mM NaCl (g)  1DWRoot/Plant − Cold (g)  2 DW Root/Plant − Low Nitrogen g)  3 DWRoot/Plant − Normal (g)  4 DW Shoot/Plant − Low Nitrogen (g)  5 DWShoot/Plant − 100 mM NaCl (g)  6 DW Shoot/Plant − Cold (g)  7 DWShoot/Plant − Normal (g)  8 Leaf number TP1 − Cold  9 Leaf number TP2 −Cold 10 Leaf number TP3 − Cold 11 Low N − total biomass (g) 12 Low N −Shoot/Root 13 Low N − roots DW (g) 14 Low N − shoots DW (g) 15 LowN-percent-root biomass compared to normal 16 Low N-percent-shoot biomasscompared to normal 17 Low N-percent-total biomass reduction compared to18 normal N level/Leaf [Low Nitrogen] 19 N level/Leaf [100 mM NaCl] 20 Nlevel/Leaf [Cold] 21 N level/Leaf [Normal] 22 Normal − Shoot/Root (g) 23Normal − roots DW (g) 24 Normal − shoots DW (g) 25 Normal − totalbiomass (g) 26 Plant Height TP1 − Cold (cm) 27 Plant Height TP2 − Cold(cm) 28 RGR Leaf Num Normal 29 Root Biomass [DW − gr.]/SPAD [100 mMNaCl] 30 Root Biomass [DW − gr.]/SPAD [Cold] 31 Root Biomass [DW −gr.]/SPAD [Low Nitrogen] 32 Root Biomass [DW − gr.]/SPAD [Normal] 33SPAD − Cold 34 SPAD − Low Nitrogen 35 SPAD − Normal 36 SPAD 100 − mMNaCl 37 Shoot Biomass [DW − gr.]/SPAD [100 mM NaCl] 38 Shoot Biomass [DW− gr.]/SPAD [Cold] 39 Shoot Biomass [DW − gr.]/SPAD [Low Nitrogen] 40Shoot Biomass [DW − gr.]/SPAD [Normal] 41 Total Biomass; Root + Shoot[DW − gr.]/ 42 SPAD [100 mM NaCl] Total Biomass; Root + Shoot [DW −gr.]/SPAD 43 [Cold] Total Biomass; Root + Shoot [DW − gr.]/ 44 SPAD [LowNitrogen] Total Biomass; Root + Shoot [DW − gr.]/ 45 SPAD [Normal] Table15: Provided are the Sorghum correlated parameters. Cold conditions = 10± 2° C.; NaCl = 100 mM NaCl; low nitrogen = 1.2 mM Nitrogen; Normalconditions = 16 mM Nitrogen.

TABLE 16 Sorghum accessions, measured parameters Eco- type/ Treat Line-Line- Line- Line- Line- Line- Line- Line- Line- Line- ment 1 2 3 4 5 6 78 9 10  1 0.05 0.10 0.12 0.07 0.08 0.08 0.14 0.10 0.16 0.14  2 0.07 0.110.16 0.09 0.08 0.11 0.14 0.13 0.11 0.14  3 0.04 0.11 0.20 0.10 0.08 0.090.13 0.09 0.09 0.09  4 0.05 0.13 0.17 0.10 0.11 0.12 0.14 0.12 0.10 0.11 5 0.08 0.19 0.33 0.16 0.16 0.16 0.26 0.20 0.13 0.18  6 0.09 0.19 0.200.14 0.13 0.13 0.15 0.19 0.10 0.12  7 0.08 0.15 0.19 0.11 0.13 0.16 0.150.15 0.11 0.14  8 0.101 0.236 0.313 0.158 0.194 0.188 0.241 0.244 0.1850.242  9 3.00 3.00 3.50 3.17 3.40 3.20 3.13 3.07 3.07 3.00 10 3.90 4.134.63 4.17 4.27 4.23 4.20 4.30 4.17 4.00 11 4.73 5.33 5.43 5.50 5.33 5.074.50 5.40 5.37 5.18 12 27.53 64.12 115.23 58.02 52.22 35.10 84.57 63.7347.03 60.00 13 1.87 1.71 1.73 1.57 2.10 1.81 2.06 2.10 1.50 2.00 14 9.6523.54 43.88 22.58 16.89 12.44 28.19 20.53 18.76 20.09 15 17.88 40.5971.35 35.44 35.33 22.66 56.38 43.20 28.27 39.91 16 84.53 80.95 117.00100.52 72.54 71.78 93.47 76.05 86.82 80.51 17 81.57 79.16 104.75 103.5083.71 83.22 107.69 81.39 70.30 75.86 18 82.58 79.81 109.10 102.32 79.7478.77 102.49 79.59 76.07 77.36 19 6.89 6.57 6.31 7.45 6.89 5.87 6.156.05 7.68 6.74 20 8.18 8.50 6.12 6.98 8.49 6.92 7.76 7.08 8.60 8.17 216.05 5.68 4.98 5.87 5.30 5.90 7.21 5.30 5.91 5.70 22 5.01 5.00 4.82 5.024.31 4.29 5.37 4.25 5.87 5.53 23 1.98 1.94 1.90 1.59 1.81 1.58 1.76 1.991.89 2.20 24 0.86 2.19 2.83 1.69 1.76 1.96 2.27 2.04 1.09 1.88 25 1.653.87 5.14 2.58 3.18 3.08 3.95 4.00 2.02 3.97 26 2.51 6.06 7.96 4.28 4.945.04 6.22 6.04 3.11 5.85 27 6.50 8.77 10.40 6.80 9.03 9.00 7.97 9.176.50 7.23 28 11.17 15.87 18.43 12.20 16.03 14.63 14.60 17.27 13.43 13.9129 0.155 0.186 0.159 0.173 0.171 0.168 0.174 0.171 0.174 0.204 30 0.0020.003 0.004 0.002 0.002 0.003 0.004 0.003 0.005 0.004 31 0.002 0.0040.006 0.003 0.003 0.004 0.004 0.004 0.003 0.005 32 0.002 0.004 0.0070.003 0.003 0.003 0.005 0.003 0.003 0.003 33 0.002 0.005 0.006 0.0040.004 0.005 0.005 0.005 0.003 0.003 34 28.62 30.31 27.04 32.28 28.2829.89 32.47 28.63 31.71 29.56 35 26.88 28.02 29.64 31.52 29.61 26.8228.48 28.21 30.48 27.63 36 26.70 29.33 29.86 29.09 24.98 24.62 30.7925.50 32.89 33.54 37 32.73 35.14 27.97 30.93 34.53 29.99 32.09 31.8632.51 34.32 38 0.003 0.005 0.007 0.004 0.004 0.004 0.005 0.006 0.0030.004 39 0.003 0.005 0.007 0.003 0.005 0.006 0.005 0.005 0.004 0.005 400.003 0.007 0.011 0.005 0.005 0.006 0.009 0.007 0.004 0.007 41 0.0040.008 0.010 0.005 0.008 0.008 0.008 0.010 0.006 0.007 42 0.004 0.0080.012 0.007 0.006 0.007 0.009 0.009 0.008 0.008 43 0.005 0.009 0.0130.006 0.008 0.009 0.009 0.010 0.007 0.009 44 0.005 0.011 0.018 0.0080.008 0.009 0.014 0.010 0.007 0.010 45 0.006 0.013 0.016 0.009 0.0120.012 0.012 0.014 0.009 0.011 Table 16: Provided are the measuredparameters under 100 mM NaCl and low temperature (8-10° C.) conditionsof Sorghum accessions (Seed ID) according to the Correlation ID numbers(described in Table 15 above)

TABLE 17 Correlation between the expression level of selected genes ofsome embodiments of the invention in roots and the phenotypicperformance under normal or abiotic stress conditions across Sorghumaccessions Gene Exp. Corr. Gene Exp. Corr. Name R P value set Set IDName R P value set Set ID LYM1195 0.80 5.68E−03 1 34 LYM1196 0.713.36E−02 7 42 LYM1201 0.75 2.02E−02 6 35 LYM1204 0.93 2.01E−03 3 35LYM1204 0.89 6.50E−03 3 19 LYM1204 0.80 9.81E−03 6 35 LYM1207 0.871.10E−02 3 35 LYM1207 0.73 2.43E−02 5 7 LYM1207 0.76 1.76E−02 5 27LYM1207 0.82 6.91E−03 5 28 LYM1207 0.77 1 53E−02 5 10 LYM1208 0.732.58E−02 6 32 LYM1208 0.74 2.36E−02 6 14 LYM1208 0.74 2.36E−02 6 3LYM1208 0.71 3.05E−02 6 12 LYM1212 0.75 1.99E−02 8 29 LYM1212 0.721.84E−02 1 11 LYM1213 0.71 3.22E−02 2 22 LYM1213 0.81 7.93E−03 5 27LYM1213 0.73 2.65E−02 5 28 LYM1213 0.75 2.01E−02 5 10 LYM1214 0.717.54E−02 3 17 LYM1214 0.81 7.60E−03 5 10 Table 17. Provided are thecorrelations (R) between the expression levels yield improving genes andtheir homologues in various tissues [Expression sets (Exp)] and thephenotypic performance [yield, biomass, growth rate and/or vigorcomponents (Correlation vector)] under abiotic stress conditions(salinity) or normal conditions across Sorghum accessions.Corr.-Correlation vector as described hereinabove (Table 15). P = pvalue.

Example 5 Production of Maize Transcriptome and High ThroughputCorrelation Analysis Using 60K Maize Oligonucleotide Micro-Array

To produce a high throughput correlation analysis, the present inventorsutilized a Maize oligonucleotide micro-array, produced by AgilentTechnologies [(chem. (dot) agilent (dot) com/Scripts/PDS (dot)asp?1Page=50879]. The array oligonucleotide represents about 60K Maizegenes and transcripts designed based on data from Public databases(Example 1). To define correlations between the levels of RNA expressionand yield, biomass components or vigor related parameters, various plantcharacteristics of 12 different Maize hybrids were analyzed. Among them,10 hybrids encompassing the observed variance were selected for RNAexpression analysis. The correlation between the RNA levels and thecharacterized parameters was analyzed using Pearson correlation test[davidmlane (dot) com/hyperstat/A34739 (dot) html].

Experimental Procedures

Five tissues at different developmental stages including Ear (flowering-R1), leaf (flowering -R1), Leaf Grain from the basal ear part, Grainfrom the distal ear, representing different plant characteristics, weresampled and RNA was extracted as described in “GENERAL EXPERIMENTAL ANDBIOINFORMATICS METHODS”. For convenience, each micro-array expressioninformation tissue type has received a Set ID as summarized in Table 18below.

TABLE 18 Tissues used for Maize transcriptome expression sets ExpressionSet Set ID Ear under normal conditions at reproductive stage: R1-R2 1Ear under normal conditions at reproductive stage: R3-R4 2 Internodeunder normal conditions at vegetative stage: V2-V3 3 Internode undernormal conditions at reproductive stage: R1-R2 4 Internode under normalconditions at vegetative stage: R3-R4 5 Leaf under normal conditions atvegetative stage: V2-V3 6 Leaf under normal conditions at reproductivestage: R1-R2 7 Grain distal under normal conditions at reproductivestage: R4-R5 Table 18: Provided are the identification (ID) number ofeach of the Maize expression sets. The following parameters werecollected:

Grain Area (cm²)—At the end of the growing period the grains wereseparated from the ear. A sample of ˜200 grains were weight,photographed and images were processed using the below described imageprocessing system. The grain area was measured from those images and wasdivided by the number of grains.

Grain Length and Grain width (cm)—At the end of the growing period thegrains were separated from the ear. A sample of ˜200 grains wereweighted, photographed and images were processed using the belowdescribed image processing system. The sum of grain lengths/or width(longest axis) was measured from those images and was divided by thenumber of grains.

Ear Area (cm²)—At the end of the growing period 6 ears were,photographed and images were processed using the below described imageprocessing system. The Ear area was measured from those images and wasdivided by the number of Ears.

Ear Length and Ear Width (cm)—At the end of the growing period 6 earswere photographed and images were processed using the below describedimage processing system. The Ear length and width (longest axis) wasmeasured from those images and was divided by the number of ears.

Filled per Whole Ear—it was calculated as the length of the ear withgrains out of the total ear.

Percent Filled Ear—At the end of the growing period 6 ears werephotographed and images were processed using the below described imageprocessing system. The percent filled Ear grain was the ear with grainsout of the total ear and was measured from those images and was dividedby the number of Ears.

The image processing system was used, which consists of a personaldesktop computer (Intel P4 3.0 GHz processor) and a public domainprogram—ImageJ 1.37, Java based image processing software, which wasdeveloped at the U.S. National Institutes of Health and is freelyavailable on the internet at rsbweb (dot) nih (dot) gov/.

Images were captured in resolution of 10 Mega Pixels (3888×2592 pixels)and stored in a low compression JPEG (Joint Photographic Experts Groupstandard) format. Next, image processing output data for seed area andseed length was saved to text files and analyzed using the JMPstatistical analysis software (SAS institute).

Additional parameters were collected either by sampling 6 plants perplot or by measuring the parameter across all the plants within theplot.

Normalized Grain Weight per plant (gr.)(yield)—At the end of theexperiment all ears from plots within blocks A-C were collected. 6 earswere separately threshed and grains were weighted, all additional earswere threshed together and weighted as well. The grain weight wasnormalized using the relative humidity to be 0%. The normalized averagegrain weight per ear was calculated by dividing the total normalizedgrain weight by the total number of ears per plot (based on plot). Incase of 6 ears, the total grains weight of 6 ears was divided by 6.

Ear FW (gr.)—At the end of the experiment (when ears were harvested)total and 6 selected ears per plots within blocks A-C were collectedseparately. The plants with (total and 6) were weighted (gr.) separatelyand the average ear per plant was calculated for total (Ear FW per plot)and for 6 (Ear FW per plant).

Plant height and Ear height—Plants were characterized for height atharvesting. In each measure, 6 plants were measured for their heightusing a measuring tape. Height was measured from ground level to top ofthe plant below the tassel. Ear height was measured from the groundlevel to the place were the main ear is located.

Leaf number per plant—Plants were characterized for leaf number duringgrowing period at 5 time points. In each measure, plants were measuredfor their leaf number by counting all the leaves of 3 selected plantsper plot.

Relative Growth Rate—RGR of leaf number was performed using Formula VIIIabove (measured in grams per day).

SPAD—Chlorophyll content was determined using a Minolta SPAD 502chlorophyll meter and measurement was performed 64 days post sowing.SPAD meter readings were done on young fully developed leaf. Threemeasurements per leaf were taken per plot. Data were taken after 46 and54 days after sowing (DPS).

Dry weight per plant—At the end of the experiment when all vegetativematerial from plots within blocks A-C were collected, weight and dividedby the number of plants.

Ear diameter [cm]—The diameter of the ear at the mid of the ear wasmeasured using a ruler.

Cob diameter [cm]—The diameter of the cob without grains was measuredusing a ruler.

Kernel Row Number per Ear—The number of rows in each ear was counted.The average of 6 ears per plot was calculated.

Leaf area index [LAI]=total leaf area of all plants in a plot.Measurement was performed using a Leaf area-meter.

Yield/LA1 [kg]—is the ratio between total grain yields and total leafarea index.

TABLE 19 Maize correlated parameters (vectors) Correla- tion Correlatedparameter with ID Cob Diameter [mm]  1 DW per Plant based on 6 [gr]  2Ear Area [cm²]  3 Ear FW per Plant based on 6 [gr]  4 Ear Height [cm]  5Ear Length [cm]  6 Ear Width [cm]  7 Ears FW per plant based on all [gr] 8 Filled per Whole Ear [value]  9 Grain Area [cm²] 10 Grain Length [cm]11 Grain Width [cm] 12 Growth Rate Leaf Num [gr/day] 13 Kernel RowNumber per Ear 14 Leaf Number per Plant 15 Normalized Grain Weight perPlant based on all [gr] 16 Normalized Grain Weight per plant based on 6[gr] 17 Percent Filled Ear 18 Plant Height per Plot [cm] 19 SPAD R1 20SPAD R2 21 Table 19.

Twelve maize varieties were grown, and characterized for parameters, asdescribed above. The average for each parameter was calculated using theJMP software, and values are summarized in Tables 20-21 below.Subsequent correlation between the various transcriptome sets for all orsub set of lines was done by the bioinformatic unit and results wereintegrated into the database (Table 22 below).

TABLE 20 Measured parameters in Maize Hybrid Eco- type/ Treat- mentLine-1 Line-2 Line-3 Line-4 Line-5 Line-6  1 28.96 25.08 28.05 25.7328.72 25.78  2 657.50 491.67 641.11 580.56 655.56 569.44  3 85.06 85.8490.51 95.95 91.62 72.41  4 245.83 208.33 262.22 263.89 272.22 177.78  5135.17 122.33 131.97 114.00 135.28 94.28  6 19.69 19.05 20.52 21.3420.92 18.23  7 5.58 5.15 5.67 5.53 5.73 5.23  8 278.19 217.50 288.28247.88 280.11 175.84  9 0.916 0.922 0.927 0.917 0.908 0.950 10 0.7530.708 0.755 0.766 0.806 0.713 11 1.167 1.092 1.180 1.205 1.228 1.123 120.810 0.814 0.803 0.803 0.824 0.803 13 0.283 0.221 0.281 0.269 0.3060.244 14 16.17 14.67 16.20 15.89 16.17 15.17 15 12.00 11.11 11.69 11.7811.94 12.33 16 153.90 135.88 152.50 159.16 140.46 117.14 17 140.68139.54 153.67 176.98 156.61 119.67 18 80.62 86.76 82.14 92.71 80.3882.76 19 278.08 260.50 275.13 238.50 286.94 224.83 20 51.67 56.41 53.5555.21 55.30 59.35 21 54.28 57.18 56.01 59.68 54.77 59.14 Table 20.

TABLE 21 Measured parameters in Maize Hybrid additional parametersEcotype/ Treatment Line-7 Line-8 Line-9 Line-10 Line-11 Line-12  1 26.4325.19 26.67  2 511.11 544.44 574.17 522.22  3 74.03 76.53 55.20 95.36  4188.89 197.22 141.11 261.11  5 120.94 107.72 60.44 112.50  6 19.02 18.5716.69 21.70  7 5.22 5.33 4.12 5.58  8 192.47 204.70 142.72 264.24  90.873 0.939 0.80 0.96 10 0.714 0.753 0.50 0.76 11 1.139 1.134 0.92 1.1812 0.791 0.837 0.67 0.81 13 0.244 0.266 0.19 0.30 14 16.00 14.83 14.2715.39 15 12.44 12.22 9.28 12.56 16 123.24 131.27 40.84 170.66 17 119.69133.51 54.32 173.23 18 73.25 81.06 81.06 91.60 19 264.44 251.61 163.78278.44 20 58.48 55.88 52.98 53.86 59.75 49.99 21 57.99 60.36 54.77 51.3961.14 53.34 Table 21.

TABLE 22 Correlation between the expression level of selected genes ofsome embodiments of the invention in various tissues and the phenotypicperformance under normal conditions across maize varieties Gene Exp.Corr. Gene Exp. Corr. Name R P value set Set ID Name R P value set SetID LYM1129 0.74 9.31E−02 2 2 LYM1130 0.71 4.65E−02 5 14 LYM1130 0.801.82E−02 5 13 LYM1130 0.76 2.83E−02 5 11 LYM1130 0.85 3.30E−02 2 3LYM1130 0.72 1.04E−01 2 16 LYM1130 0.72 1.09E−01 2 13 LYM1130 0.805.77E−02 2 11 LYM1130 0.78 6.72E−02 2 6 LYM1130 0.82 4.41E−02 2 7LYM1130 0.75 8.40E−02 2 8 LYM1130 0.82 4.40E−02 2 4 LYM1130 0.834.19E−02 2 17 LYM1131 0.78 3.69E−02 4 5 LYM1131 0.78 4.01E−02 7 3LYM1131 0.73 6.39E−02 7 16 LYM1131 0.86 1.37E−02 7 5 LYM1131 0.755.46E−02 7 17 LYM1131 0.75 5.29E−02 1 10 LYM1131 0.78 3.71E−02 1 5LYM1131 0.72 7.06E−02 1 7 LYM1131 0.74 5.64E−02 1 12 LYM1131 0.831.10E−02 8 1 LYM1131 0.77 2.56E−02 8 14 LYM1131 0.83 1.15E−02 8 13LYM1131 0.80 1.81E−02 8 11 LYM1131 0.71 4.81E−02 8 10 LYM1131 0.782.17E−02 8 2 LYM1131 0.72 4.42E−02 8 7 LYM1131 072 2.91E−02 3 14 LYM11320.81 1.55E−02 8 1 LYM1132 0.73 3.82E−02 8 14 LYM1133 0.76 7.73E−02 7 1LYM1133 0.71 7.31E−02 7 18 LYM1133 0.73 1.59E−02 6 18 LYM1133 0.882.14E−02 2 9 LYM1133 0.85 3.03E−02 2 18 LYM1134 0.91 1.20E−02 1 1LYM1134 0.72 6.55E−02 1 2 LYM1134 0.89 1.67E−02 2 12 LYM1136 0.874.71E−03 5 11 LYM1136 0.73 6.26E−02 1 9 LYM1136 0.74 5.80E−02 1 12LYM1136 0.90 1.31E−02 2 12 LYM1137 0.78 6.98E−02 1 1 LYM1137 0.736.39E−02 1 8 LYM1137 0.84 4.65E−03 3 2 LYM1138 0.73 3.90E−02 8 13LYM1138 088 4.36E−03 8 11 LYM1138 0.81 1.55E−02 8 6 LYM1138 0.856.83E−03 8 10 LYM1138 0.75 3.12E−02 8 7 LYM1138 0.75 3.30E−02 8 4LYM1138 0.71 4.64E−02 8 17 LYM1138 0.72 1.04E−01 2 3 LYM1138 0.749.30E−02 2 14 LYM 138 0.84 3.49E−02 2 11 LYM1138 0.78 6.99E−02 2 19LYM1138 0.83 3.99E−02 2 2 LYM1138 0.79 6.02E−02 2 5 LYM1138 0.805.61E−02 2 7 LYM1138 0.82 4.40E−02 2 8 LYM1138 0.81 5.32E−02 2 4 LYM11390.75 5.45E−02 4 3 LYM1139 0.80 2.95E−02 4 14 LYM1139 0.72 6.84E−02 4 11LYM1139 0.73 6.09E−02 4 6 LYM1139 0.73 6.44E−02 4 4 LYM1139 0.726.84E−02 4 17 LYM1139 0.78 3.99E−02 7 3 LYM1139 0.78 4.04E−02 7 14LYM1139 0.73 6.44E−02 7 6 LYM1139 0.75 5.29E−02 7 4 LYM1139 0.726.56E−02 7 17 LYM1139 0.89 7.52E−03 1 3 LYM1139 0.79 3.62E−02 1 16LYM1139 0.90 5.70E−03 1 6 LYM1139 0.82 2.46E−02 1 18 LYM1139 0.822.51E−02 1 8 LYM1139 0.86 1.23E−02 1 4 LYM1139 0.81 2.78E−02 1 17LYM1139 0.79 6.14E−02 2 12 LYM1140 0.71 1.15E−01 1 1 LYM1140 0.812.79E−02 1 2 LYM1140 0.72 1.10E−01 2 3 LYM1140 0.71 1.17E−01 2 11LYM1140 0.71 1.11E−01 2 6 LYM1141 0.72 6.82E−02 4 15 LYM1141 0.812.67E−02 4 9 LYM1141 0.83 2.04E−02 7 3 LYM1141 0.89 7.39E−03 7 16LYM1141 0.81 2.72E−02 7 14 LYM1141 0.84 1.70E−02 7 15 LYM1141 0.736.07E−02 7 13 LYM1141 0.76 4.85E−02 7 21 LYM1141 0.94 1.61E−03 7 11LYM1141 0.79 3.51E−02 7 6 LYM1141 0.87 1.14E−02 7 9 LYM1141 0.914.66E−03 7 10 LYM1141 0.75 5.12E−02 7 19 LYM1141 0.78 3.71E−02 7 5LYM1141 0.92 3.00E−03 7 7 LYM1141 0.71 7.32E−02 7 8 LYM1141 0.812.85E−02 7 12 LYM1141 0.77 4.26E−02 7 4 LYM1141 0.88 8.42E−03 7 17LYM1141 0.79 3.52E−02 1 16 LYM1141 0.88 8.30E−03 1 15 LYM1141 0.888.47E−03 1 11 LYM1141 0.88 8.55E−03 1 9 LYM1141 0.93 2.80E−03 1 10LYM1141 0.73 6.22E−02 1 19 LYM1141 0.75 5.24E−02 1 5 LYM1141 0.871.13E−02 1 7 LYM1141 0.92 2.94E−03 1 12 LYM1141 0.77 4.46E−02 1 17LYM1141 0.77 2.51E−02 8 13 LYM1141 0.71 4.62E−02 8 11 LYM1141 0.753.30E−02 8 10 LYM1142 0.78 6.74E−02 2 15 LYM1142 0.76 7.68E−02 2 12LYM1143 0.88 4.10E−03 5 15 LYM1143 0.71 4.71E−02 5 18 LYM1143 0.701.20E−01 4 1 LYM1143 0.73 6.38E−02 4 16 LYM1143 0.77 4.23E−02 4 14LYM1143 0.82 2.53E−02 4 15 LYM1143 0.90 5.51E−03 4 13 LYM1143 0.851.59E−02 4 11 LYM1143 0.73 5.99E−02 4 6 LYM1143 0.77 4.32E−02 4 10LYM1143 0.79 3.32E−02 4 7 LYM1143 0.70 7.82E−02 4 4 LYM1143 0.745.59E−02 4 17 LYM1143 0.72 6.71E−02 7 3 LYM1143 0.72 6.59E−02 7 16LYM1143 0.87 1.18E−02 7 14 LYM1143 0.83 2.15E−02 7 13 LYM1143 0.841.82E−02 7 11 LYM1143 0.82 2.53E−02 7 6 LYM1143 0.72 7.08E−02 7 10LYM1143 0.76 4.58E−02 7 7 LYM1143 0.80 3.10E−02 7 4 LYM1143 0.783.93E−02 7 17 LYM1143 0.82 2.39E−02 1 14 LYM1143 0.72 6.96E−02 1 15LYM1143 0.81 2.71E−02 1 13 LYM1143 0.78 3.82E−02 1 11 LYM1143 0.726.64E−02 1 10 LYM1143 0.77 4.34E−02 1 7 LYM1143 0.73 4.00E−02 8 16LYM1143 0.76 2.94E−02 8 13 LYM1143 0.76 2.81E−02 8 10 LYM1143 0.724.38E−02 8 5 LYM1143 0.74 3.40E−02 8 7 LYM1143 0.78 2.21E−02 8 8 LYM11430.75 3.07E−02 8 4 LYM1146 0.73 1.01E−01 2 9 LYM1146 0.70 1.20E−01 2 10LYM1146 0.94 5.60E−03 2 12 LYM1149 0.72 6.98E−02 7 3 LYM1149 0.707.97E−02 7 16 LYM1149 0.73 6.11E−02 7 19 LYM1149 0.77 4.17E−02 1 19LYM1149 0.79 3.45E−02 1 12 LYM1149 0.73 4.05E−02 8 12 LYM1149 0.742.34E−02 3 16 LYM1149 0.72 3.03E−02 3 12 LYM1149 0.83 3.95E−02 2 12LYM1151 0.73 4.04E−02 5 9 LYM1151 0.73 6.32E−02 4 14 LYM1151 0.78374E−02 7 2 LYM1151 0.74 9.14E−02 1 1 LYM1151 0.70 7.71E−02 1 2 LYM11510.71 1.14E−01 2 6 LYM1151 0.80 5.47E−02 2 18 LYM1151 0.74 9.38E−02 2 17LYM1152 0.85 7.44E−03 5 14 LYM1152 0.85 8.12E−03 5 11 LYM1152 0.724.25E−02 5 6 LYM1152 0.75 8.79E−02 4 1 LYM1152 0.75 4.98E−02 4 2 LYM11520.74 9.37E−02 2 12 LYM1153 0.73 2.43E−02 3 14 LYM1153 0.78 6.99E−02 2 3LYM1153 0.74 9.08E−02 2 16 LYM1153 0.77 7.26E−02 2 13 LYM1153 0.711.13E−01 2 11 LYM1153 0.76 7.86E−02 2 6 LYM1153 0.83 4.13E−02 2 10LYM1153 0.82 4.65E−02 2 5 LYM1153 0.71 1.12E−01 2 4 LYM1153 0.833.87E−02 2 17 LYM1154 0.77 2.44E−02 5 19 LYM1154 0.77 4.23E−02 4 2LYM1154 0.80 5.55E−02 1 1 LYM1154 0.80 5.84E−03 6 18 LYM1154 0.945.21E−03 2 15 LYM1155 0.74 9.23E−02 4 1 LYM1155 0.74 5.88E−02 4 2LYM1156 0.73 6.21E−02 4 2 LYM1156 0.91 1.14E−02 1 1 LYM1156 0.774.35E−02 1 2 LYM1156 0.75 2.00E−02 3 2 LYM1156 0.78 6.96E−02 2 3 LYM11560.71 1.11E−01 2 11 LYM1156 0.76 8.26E−02 2 6 LYM1156 0.72 1.08E−01 2 4LYM1156 0.79 6.14E−02 2 17 LYM1158 0.79 2.03E−02 5 3 LYM1158 0.839.97E−03 5 16 LYM1158 0.79 1.96E−02 5 13 LYM1158 0.78 2.29E−02 5 11LYM1158 0.81 1.38E−02 5 6 LYM1158 0.80 1.74E−02 5 10 LYM1158 0.762.77E−02 5 19 LYM1158 0.70 5.20E−02 5 5 LYM1158 0.92 1.09E−03 5 7LYM1158 0.94 6.40E−04 5 8 LYM1158 0.91 1.85E−03 5 4 LYM1158 0.762.93E−02 5 17 LYM1158 0.75 8.50E−02 4 1 LYM1158 0.83 2.15E−02 7 14LYM1158 0.77 4.48E−02 7 6 LYM1158 0.77 4.46E−02 7 18 LYM1158 0.764.95E−02 7 4 LYM1158 0.75 8.41E−02 1 1 LYM1158 0.76 8.13E−02 2 3 LYM11580.87 2.39E−02 2 6 LYM1158 0.79 6.12E−02 2 18 LYM1158 0.79 5.95E−02 2 17LYM1159 0.72 3.01E−02 6 1 LYM1159 0.90 1.05E−03 3 2 LYM1160 0.897.09E−03 7 18 LYM1161 0.85 1.52E−02 4 3 LYM1161 0.91 4.55E−03 4 16LYM1161 0.78 3.83E−02 4 14 LYM1161 0.84 1.85E−02 4 15 LYM1161 0.783.91E−02 4 13 LYM1161 0.88 8.96E−03 4 11 LYM1161 0.78 3.94E−02 4 6LYM1161 0.90 5.38E−03 4 9 LYM1161 0.88 8.73E−03 4 10 LYM1161 0.905.68E−03 4 19 LYM1161 0.85 1.53E−02 4 5 LYM1161 0.92 3.17E−03 4 7LYM1161 0.80 3.19E−02 4 8 LYM1161 0.81 2.71E−02 4 12 LYM1161 0.793.43E−02 4 4 LYM1161 0.86 1.35E−02 4 17 LYM1161 0.82 2.55E−02 1 19LYM1161 0.90 5.18E−03 1 5 LYM1161 0.73 6.03E−02 1 7 LYM1161 0.755.20E−02 1 8 LYM1161 0.71 2.12E−02 6 15 LYM1161 0.78 1.41E−02 3 3LYM1161 0.82 6.91E−03 3 16 LYM1161 0.74 2.33E−02 3 14 LYM1161 0.761.70E−02 3 13 LYM1161 0.81 7.63E−03 3 11 LYM1161 0.76 1.63E−02 3 6LYM1161 0.79 1.10E−02 3 10 LYM1161 0.83 5.44E−03 3 19 LYM1161 0.809.13E−03 3 5 LYM1161 0.85 3.42E−03 3 7 LYM1161 0.81 7.99E−03 3 8 LYM11610.79 1.08E−02 3 4 LYM1161 0.80 9.45E−03 3 17 LYM1161 0.78 6.79E−02 2 3LYM1161 0.74 9.49E−02 2 16 LYM1161 0.85 3.30E−02 2 11 LYM1161 0.872.32E−02 2 6 LYM1161 0.79 6.33E−02 2 19 LYM1161 0.77 7.61E−02 2 4LYM1162 0.92 3.41E−03 4 14 LYM1162 0.78 3.86E−02 4 6 LYM1162 0.851.43E−02 4 8 LYM1162 0.85 1.66E−02 4 4 LYM1162 0.72 6.78E−02 1 5 LYM11620.91 1.23E−02 2 12 LYM1163 0.74 5.72E−02 4 3 LYM1163 0.79 3.60E−02 4 16LYM1163 0.79 3.28E−02 4 15 LYM1163 0.82 2.32E−02 4 11 LYM1163 0.783.96E−02 4 9 LYM1163 0.84 1.78E−02 4 10 LYM1163 0.80 2.90E−02 4 5LYM1163 0.78 3.76E−02 4 7 LYM1163 0.86 1.40E−02 4 12 LYM1163 0.793.55E−02 4 17 LYM1163 0.76 2.85E−02 8 1 LYM1163 0.71 1.17E−01 2 9LYM1163 0.93 7.22E−03 2 12 LYM1165 0.74 5.77E−02 4 3 LYM1165 0.727.06E−02 4 16 LYM1165 0.73 6.18E−02 4 14 LYM1165 0.75 5.07E−02 4 11LYM1165 0.74 5.81E−02 4 10 LYM1165 0.86 1.37E−02 4 5 LYM1165 0.764.87E−02 4 7 LYM1165 0.73 6.34E−02 4 17 LYM1165 0.73 6.46E−02 7 3LYM1165 0.77 4.33E−02 7 5 LYM1165 0.78 3.82E−02 1 16 LYM1165 0.727.04E−02 1 15 LYM1165 0.80 2.92E−02 1 11 LYM1165 0.80 3.10E−02 1 9LYM1165 0.87 1.14E−02 1 10 LYM1165 0.85 1.49E−02 1 19 LYM1165 0.923.56E−03 1 5 LYM1165 0.87 1.14E−02 1 7 LYM1165 0.71 7.20E−02 1 8 LYM11650.88 9.60E−03 1 12 LYM1165 0.72 6.77E−02 1 17 LYM1165 0.71 2.11E−02 6 12LYM1167 0.80 5.75E−02 1 1 LYM1167 0.89 1.67E−02 2 9 LYM1167 0.901.58E−02 2 18 LYM1168 0.73 6.08E−02 4 2 LYM1169 0.78 2.34E−02 5 11LYM1169 0.76 2.78E−02 5 9 LYM1169 0.71 7.15E−02 4 3 LYM1169 0.812.57E−02 4 19 LYM1169 0.85 1.58E−02 4 5 LYM1169 0.84 1.91E−02 4 8LYM1169 0.72 7.00E−02 4 4 LYM1169 0.74 9.03E−02 2 9 LYM1169 0.843.86E−02 2 12 LYM1170 0.90 5.92E−03 4 3 LYM1170 0.90 5.52E−03 4 16LYM1170 0.75 5.20E−02 4 14 LYM1170 0.72 6.76E−02 4 15 LYM1170 0.745.68E−02 4 13 LYM1170 0.85 1.60E−76 4 11 LYM1170 0.86 1.24E−02 4 6LYM1170 0.79 3.50E−02 4 9 LYM1170 0.81 2.75E−02 4 10 LYM1170 0.851.62E−02 4 19 LYM1170 0.82 2.40E−02 4 5 LYM1170 0.87 1.08E−02 4 7LYM1170 0.84 1.89E−02 4 8 LYM1170 0.85 1.53E−02 4 4 LYM1170 0.889.02E−03 4 17 LYM1171 0.77 2.49E−02 5 9 LYM1171 0.78 3.73E−02 4 15LYM1171 0.83 1.99E−02 4 9 LYM1171 0.70 7.76E−02 4 10 LYM1171 0.726.97E−02 4 7 LYM1171 0.71 7.11E−02 4 12 LYM1171 0.81 4.77E−03 6 5LYM1171 0.83 4.29E−02 2 12 LYM1172 0.82 1.28E−02 5 9 LYM1173 0.824.61E−02 2 10 LYM1173 0.79 6.01E−02 2 7 LYM1173 0.72 1.10E−01 2 8LYM1173 0.70 1.20E−01 2 4 LYM1174 0.71 4.69E−02 5 9 LYM1174 0.793.44E−02 4 3 LYM1174 0.81 2.56E−02 4 16 LYM1174 0.75 5.24E−02 4 15LYM1174 0.87 1.14E−02 4 11 LYM1174 0.73 6.31E−02 4 6 LYM1174 0.764.52E−02 4 9 LYM1174 0.83 2.23E−02 4 10 LYM1174 0.81 2.84E−02 4 7LYM1174 0.73 6.39E−02 4 12 LYM1174 0.85 1.63E−02 4 17 LYM1174 0.707.97E−02 7 2 LYM1174 0.94 4.56E−05 6 3 LYM1174 0.88 8.99E−04 6 16LYM1174 0.80 5.31E−03 6 11 LYM1174 0.88 8.90E−04 6 6 LYM1174 0.702.36E−02 6 9 LYM1174 0.77 8.75E−03 6 10 LYM1174 0.80 5.69E−03 6 7LYM1174 0.78 8.42E−03 6 8 LYM1174 0.71 2.26E−02 6 12 LYM1174 0.871.11E−03 6 4 LYM1174 0.94 6.60E−05 6 17 LYM1174 0.90 1.41E−02 2 9LYM1174 0.91 1.12E−02 2 18 LYM1175 0.75 8.67E−02 1 1 LYM1176 0.815.25E−02 2 15 LYM1177 0.86 2.72E−03 3 11 LYM1177 0.87 2.42E−03 3 10LYM1177 0.76 1.86E−02 3 19 LYM1177 0.76 1.69E−02 3 5 LYM1177 0.835.79E−03 3 7 LYM1177 0.81 8.38E−03 3 12 LYM1177 0.73 2.68E−02 3 17LYM1177 0.95 3.65E−03 2 9 LYM1177 0.88 2.09E−02 2 18 LYM1178 0.901.39E−02 4 1 LYM1178 0.79 3.61E−02 4 6 LYM1178 0.77 4.47E−02 4 2 LYM11780.76 4.58E−02 4 8 LYM1178 0.74 5.60E−02 4 4 LYM1178 0.73 6.32E−02 7 3LYM1178 0.79 3.30E−02 7 6 LYM1178 0.80 3.14E−02 7 18 LYM1178 0.945.60E−03 1 1 LYM1178 0.78 3.71E−02 1 2 LYM1179 0.70 7.83E−02 4 14LYM1179 0.76 4.82E−02 1 14 LYM1179 0.70 7.74E−02 1 11 LYM1179 0.764.95E−02 1 5 LYM1179 0.71 7.28E−02 1 7 LYM1179 0.75 3.31E−02 8 1 LYM11790.81 1.52E−02 8 2 LYM1180 0.75 5.34E−02 1 18 LYM1180 0.81 5.29E−02 2 9LYM1182 0.75 3.26E−02 5 11 LYM1182 0.70 7.98E−02 7 13 LYM1182 0.824.50E−02 2 19 LYM1183 0.77 4.39E−02 7 12 LYM1183 0.74 9.23E−02 2 12LYM1184 0.77 4.40E−02 4 2 LYM1184 0.88 2.04E−02 1 1 LYM1184 0.793.32E−02 1 2 LYM1185 0.76 7.66E−02 2 3 LYM1185 0.79 6.15E−02 2 11LYM1185 0.71 1.16E−01 2 6 LYM1185 0.84 3.77E−02 2 7 LYM1185 0.815.26E−02 2 8 LYM1185 0.80 5.57E−02 2 4 LYM1185 0.71 1.11E−01 2 17LYM1186 0.97 1.42E−03 7 1 LYM1186 0.78 3.79E−02 1 15 LYM1186 0.812.85E−02 1 9 LYM1186 0.76 4.66E−02 1 12 LYM1186 0.84 3.43E−02 2 14LYM1187 0.76 4.63E−02 5 1 LYM1187 0.72 4.56E−02 5 2 LYM1187 0.717.30E−02 7 11 LYM1187 0.70 5.18E−02 8 1 LYM1187 0.72 4.47E−02 8 13LYM1187 0.71 4.77E−02 8 10 LYM1187 0.71 3.31E−02 3 15 LYM1187 0.703.55E−02 3 12 LYM1187 0.72 2.92E−02 3 17 LYM1187 0.96 2.29E−03 2 12Table 22. Provided are the correlations (R) between the expressionlevels yield improving genes and their homologs in various tissues[Expression (Exp) sets] and the phenotypic performance [yield, biomass,growth rate and/or vigor components (Correlation vector (Cor))] undernormal conditions across maize varieties. P = p value.

Example 6 Production of Barley Transcriptome and High ThroughputCorrelation Analysis Using 60K Barley Oligonucleotide Micro-Array

In order to produce a high throughput correlation analysis comparingbetween plant phenotype and gene expression level, the present inventorsutilized a Barley oligonucleotide micro-array, produced by AgilentTechnologies [chem. (dot) agilent (dot) com/Scripts/PDS (dot)asp?1Page=50879]. The array oligonucleotide represents about 60K Barleygenes and transcripts. In order to define correlations between thelevels of RNA expression and yield or vigor related parameters, variousplant characteristics of 15 different Barley accessions were analyzed.Among them, 10 accessions encompassing the observed variance wereselected for RNA expression analysis. The correlation between the RNAlevels and the characterized parameters was analyzed using Pearsoncorrelation test [davidmlane (dot) com/hyperstat/A34739 (dot) html].

Experimental Procedures

Analyzed Barley tissues—Five tissues at different developmental stages[leaf, meristem, root tip, adventitious root and booting spike],representing different plant characteristics, were sampled and RNA wasextracted as described above. Each micro-array expression informationtissue type has received a Set ID as summarized in Tables 23 and 24below.

TABLE 23 Barley transcriptome expression sets (set I) Expression Set SetID Root at vegetative stage under low N conditions 1 Root at vegetativestage under normal conditions 2 leaf at vegetative stage under low Nconditions 3 leaf at vegetative stage under normal conditions 4 root tipat vegetative stage under low N conditions 5 root tip at vegetativestage under normal conditions 6 Table 23.

TABLE 24 Barley transcriptome expression sets (set 2) Expression Set SetID booting spike at reproductive stage under drought conditions 1 leafat reproductive stage under draught conditions 2 leaf at vegetativestage under drought conditions 3 meristems at vegetative stage underdrought conditions 4 root tip at vegetative stage under droughtconditions 5 root tip at vegetative stage under recovery drought 6 Table24.

Barley yield components and vigor related parameters assessment—15Barley accessions in 5 repetitive blocks, each containing 5 plants perpot were grown at net house. Three different treatments were applied:plants were regularly fertilized and watered during plant growth untilharvesting (as recommended for commercial growth) or under low Nitrogen(80% percent less Nitrogen) or drought stress. Plants were phenotyped ona daily basis following the standard descriptor of barley (Tables 25 and26, below). Harvest was conducted while all the spikes were dry. Allmaterial was oven dried and the seeds were threshed manually from thespikes prior to measurement of the seed characteristics (weight andsize) using scanning and image analysis. The image analysis systemincluded a personal desktop computer (Intel P4 3.0 GHz processor) and apublic domain program—ImageJ 1.37 (Java based image processing program,which was developed at the U.S. National Institutes of Health and freelyavailable on the internet [rsbweb (dot) nih (dot) gov/]. Next, analyzeddata was saved to text files and processed using the JMP statisticalanalysis software (SAS institute).

Grains number—The total number of grains from all spikes that weremanually threshed was counted. Number of grains per plot were counted.

Grain weight (gr.)—At the end of the experiment all spikes of the potswere collected. The total grains from all spikes that were manuallythreshed were weighted. The grain yield was calculated by per plot.

Spike length and width analysis—At the end of the experiment the lengthand width of five chosen spikes per plant were measured using measuringtape excluding the awns.

Spike number analysis—The spikes per plant were counted.

Plant height—Each of the plants was measured for its height usingmeasuring tape. Height was measured from ground level to top of thelongest spike excluding awns at two time points at the Vegetative growth(30 days after sowing) and at harvest.

Spike weight—The biomass and spikes weight of each plot was separated,measured and divided by the number of plants.

Dry weight=total weight of the vegetative portion above ground(excluding roots) after drying at 70° C. in oven for 48 hours at twotime points at the Vegetative growth (30 days after sowing) and atharvest.

Root dry weight=total weight of the root portion underground afterdrying at 70° C. in oven for 48 hours at harvest.

Root/Shoot Ratio—Root/Shoot Ratio (=RBiH/BiH) was performed usingFormula XXII above.

Total No of tillers—all tillers were counted per plot at two time pointsat the Vegetative growth (30 days after sowing) and at harvest.

SPAD—Chlorophyll content was determined using a Minolta SPAD 502chlorophyll meter and measurement was performed at time of flowering.SPAD meter readings were done on young fully developed leaf. Threemeasurements per leaf were taken per plot.

Root FW (gr.), root length (cm) and number of lateral roots—3 plants perplot were selected for measurement of root weight, root length and forcounting the number of lateral roots formed.

Shoot FW—weight of 3 plants per plot were recorded at differenttime-points.

Relative water content—Fresh weight (FW) of three leaves from threeplants each from different seed ID was immediately recorded; then leaveswere soaked for 8 hours in distilled water at room temperature in thedark, and the turgid weight (TW) was recorded. Total dry weight (DW) wasrecorded after drying the leaves at 60° C. to a constant weight.Relative water content (RWC) was calculated according to Formula Iabove.

Harvest Index (for barley)—The harvest index was calculated usingFormula XVIII above.

Relative growth rate: relative growth rate (RGR) of Plant Height. SPADand number of tillers were performed using Formula III, Formula IV andFormula V respectively.

TABLE 25 Barley correlated parameters (vectors for set 1) Corre- lationCorrelated parameter with ID Lateral Roots (number) − Normal  1 LeafArea (mm²) − Normal  2 Leaf Number-TP4 − Low N  3 Max Length (mm) −Normal  4 Max Width (mm) − Normal  5 Max Length (mm)-TP4 − Low N  6 MaxWidth (mm)-TP4 − Low N  7 No of lateral roots-Low N − TP2  8 Num Leaves− Normal  9 Num Seeds − Normal 10 Num Spikes per plot − Normal 11 NumTillers per plant − Normal 12 Plant Height (cm) − TP1-Normal 13 PlantHeight (cm) − TP2-Normal 14 Plant Height (cm)-Low N − TPI 15 PlantHeight(cm)-Low N − TP2 16 Root FW (g) − Normal 17 Root Length (cm) −Normal 18 Root FW (g)-Low N − TP2 19 Root length (cm)-Low N − TP2 20SPAD − Normal 21 SPAD-Low N − TP2 22 Seed Yield (gr) − Normal 23 SeedNumber (per plot) − Low N 24 Seed Yield (g) − Low N 25 Shoot FW (g) −Normal 26 Spike Length (cm) − Normal 27 Spike Width (cm) − Normal 28Spike weight per plot (g) − Normal 29 Spike Length (cm) − Low N 30 SpikeWidth (cm) − Low N 31 Spike total weight (pet plot) − Low N 32 TotalTillers per plot (number) − Normal 33 Total Leaf Area (mm²)-TP4 − Low N34 Total No of Spikes per plot − Low N 35 Total No of tillers per plot −Low N 36 shoot FW (gr)-Low N − TP2 37 Table 25. Provided are the barleycorrelated parameters, TP means time point, DW—dry weight, FW—freshweight and Low N—Low Nitrogen.

TABLE 26 Barley correlated parameters (vectors for set 2) Corre- lationCorrelated parameter with ID Chlorophyll levels 1 Dry weight harvest (g)2 Dry weight vegetative growth 3 Fresh weight (g) 4 Grain number 5 Grainweight (g) 6 Harvest index (value) 7 Heading date 8 Height Relativegrowth rate (cm/day) 9 Number of tillers Relative growth rate 10 (numberof tillers/day) Plant height T1 (cm) 11 Plant height T2 (cm) 12 RBiH/BiH(value) 13 Relative water content 14 Root dry weight (g) 15 Root freshweight (g) 16 Root length (cm) 17 SPAD Relative growth rate 18 Spikelength (cm) 19 Spike number per plant 20 Spike weight per plant 21 Spikewidth (cm) 27 Tillers number T1 23 Tillers number T2 24 lateral rootnumber 25 Table 26. Provided are the barley correlated parameters, TPmeans time point, DW—dry weight, FW—fresh weight and Low N—Low Nitrogen.

Experimental Results

15 different Barley accessions were grown and characterized fordifferent parameters as described above. The average for each of themeasured parameter was calculated using the JMP software and values aresummarized in Tables 27-29 below. Subsequent correlation analysisbetween the various transcriptome sets and the average parameters wasconducted (Tables 30-31). Follow, results were integrated to thedatabase.

TABLE 27 Measured parameters correlation IDs in Barley accessions(set 1) Ecotype/ Treatment Line-1 Line-2 Line-3 Line-4 Line-5 Line-6Line-7 Line-8 Line-9 Line-10 1 7.00 8.67 8.33 9.67 10.70 9.67 9.67 8.6710.00 9.67 2 294.00 199.00 273.00 276.00 313.00 309.00 259.00 291.00299.00 296.00 3 8.00 8.00 7.50 8.50 10.00 11.50 8.60 6.33 7.50 10.00 4502.00 348.00 499.00 594.00 535.00 551.00 479.00 399.00 384.00 470.00 55.77 5.45 5.80 6.03 4.63 5.33 5.83 5.43 5.75 6.03 6 102.90 107.78 111.57142.42 152.38 149.33 124.08 95.00 124.12 135.17 7 5.25 5.17 5.12 5.305.20 5.33 5.32 5.10 5.15 5.10 8 5.00 6.00 4.33 6.00 6.33 6.00 6.67 4.675.67 7.33 9 24.20 18.20 22.70 25.50 23.20 28.30 22.20 19.00 17.30 22.0010 1090.00 510.00 242.00 582.00 621.00 1070.00 903.00 950.00 984.00768.00 11 41.50 32.00 36.00 71.40 34.20 45.60 49.80 28.00 19.30 38.00 122.00 2.00 1.00 2.33 2.33 3.33 2.33 1.33 1.33 1.67 13 39.20 37.00 36.8049.80 46.80 34.80 43.20 35.70 46.20 40.20 14 64.70 84.00 67.40 82.0072.00 56.60 65.80 62.80 91.60 66.20 15 41.00 82.00 61.40 59.40 65.8047.80 53.80 56.40 81.80 44.60 16 16.33 18.83 17.33 26.00 22.50 18.1719.67 19.83 19.17 19.17 17 0.27 0.27 0.25 0.35 0.62 0.27 0.35 0.32 0.230.27 18 21.30 15.00 21.80 20.30 27.20 16.00 24.00 13.50 21.50 15.20 190.38 0.23 0.12 0.40 0.88 0.50 0.43 0.32 0.30 0.55 20 24.67 21.67 22.0021.67 22.17 23.00 30.50 22.83 23.83 24.50 21 39.10 41.40 35.20 33.7034.20 42.80 37.00 36.90 35.00 36.80 22 24.03 23.30 26.47 23.90 26.6323.20 25.43 24.23 25.03 26.07 23 46.40 19.80 10.80 22.60 30.30 54.1037.00 42.00 35.40 38.30 24 230.20 164.60 88.25 133.60 106.00 222.60219.20 143.45 201.80 125.00 25 9.76 7.31 3.30 5.06 6.02 9.74 7.35 5.807.83 6.29 26 2.17 1.90 1.25 3.00 15.60 3.02 2.58 1.75 2.18 1.82 27 16.5019.20 18.30 20.40 17.20 19.10 20.30 21.70 16.50 16.10 28 9.54 9.05 8.256.55 10.50 8.83 7.38 10.40 10.20 10.30 29 69.40 39.40 34.90 50.30 60.8079.10 62.70 60.00 55.90 59.70 30 15.19 19.61 16.30 19.32 90.22 16.4420.44 18.84 18.77 16.65 31 7.95 8.13 9.43 4.94 9.60 7.16 7.06 8.51 10.019.40 32 13.74 13.44 9.15 11.64 11.34 15.06 12.18 10.95 12.18 10.62 3346.70 41.60 40.00 48.80 34.60 48.60 49.20 29.00 27.50 38.80 34 39.4046.27 51.51 57.07 67.78 64.15 52.42 46.15 68.02 57.91 35 12.20 9.0011.60 25.00 7.80 14.50 15.00 7.00 5.40 8.40 36 16.20 14.60 16.00 20.7512.50 18.80 21.20 11.00 6.75 14.00 37 0.43 0.43 0.33 0.58 0.78 0.53 0.450.43 0.50 0.62

TABLE 28 Measured parameters of correlation IDs in Barley accessions(set 2) Ecotype/ Treatment Line-1 Line-2 Line-3 Line-4 Line-5 Line-6Line-7 Line-8 1 41.33 33.57 36.57 40.50 45.07 39.73 38.33 36.17 2 6.155.05 3.20 3.28 4.76 3.55 4.52 3.38 3 0.21 0.21 0.17 4 1.90 1.52 1.171.95 1.90 1.22 1.75 1.58 5 170.00 267.50 111.00 205.33 153.60 252.50288.40 274.50 6 5.55 9.80 3.55 7.20 5.28 7.75 9.92 10.25 7 0.47 0.660.53 0.69 0.53 0.69 0.69 0.75 8 75.00 71.00 65.00 66.75 90.00 90.00 90.27 0.86 0.73 0.88 0.40 0.94 0.70 0.71 10 0.070 0.097 0.059 0.071 0.1640.061 0.104 0.049 11 33.33 27.00 31.33 34.17 31.33 30.33 28.67 38.67 1246.00 52.80 35.00 38.00 45.20 48.00 37.67 41.20 13 0.013 0.012 0.0080.006 0.025 0.020 0.008 0.008 14 80.60 53.40 55.87 43.21 69.78 45.4976.51 15 77.52 60.19 27.13 18.62 117.42 70.72 37.34 25.56 16 2.07 1.481.12 1.87 1.67 1.68 1.62 0.85 17 21.67 20.33 22.00 24.00 20.67 18.3321.00 20.33 18 0.09 −0.12 0.00 0.01 0.04 −0.07 0.01 0.00 19 16.70 16.8513.27 13.55 14.19 15.64 15.66 17.49 20 4.20 4.36 7.60 8.44 4.92 3.436.90 5.80 21 17.72 24.24 18.20 18.00 19.50 15.00 23.40 28.16 22 8.649.07 7.82 7.32 8.74 7.62 6.98 8.05 23 2.00 2.00 1.67 1.67 2.00 1.67 2.331.00 24 11.68 9.04 10.92 10.16 10.32 8.78 13.00 7.44 25 8.33 8.67 7.337.67 6.67 6.67 7.67 6.67

TABLE 29 Measured parameters of correlation IDs in Barley accessions(set 2) additional lines Eco- type/ Treat- Line- Line- Line- Line- Line-Line- ment Line-9 10 11 12 13 14 15 1 42.13 31.77 33.47 42.37 42.2736.77 40.63 2 5.67 3.31 2.65 5.12 6.86 3.11 3.74 3 0.25 0.13 0.19 0.22 41.88 1.73 1.00 0.90 0.90 1.43 0.83 5 348.50 358.00 521.39 71.50 160.13376.67 105.00 6 8.50 14.03 17.52 2.05 5.38 11.00 2.56 7 0.60 0.81 0.870.29 0.44 0.78 0.41 8 90.00 90.00 81.60 90.00 9 0.77 0.80 0.92 0.39 0.88−0.13 0.20 10 0.100 0.061 0.063 0.183 0.149 0.022 0.442 11 33.67 28.4327.50 25.00 27.00 31.00 22.33 12 40.80 49.86 43.00 47.40 64.80 52.6032.00 13 0.012 0.007 0.016 0.023 0.012 0.012 0.026 14 87.41 58.32 80.5873.09 15 66.18 22.13 41.12 116.95 84.10 37.46 98.86 16 1.45 1.38 0.820.58 0.63 1.07 0.70 17 21.67 19.67 16.67 17.00 15.17 27.00 15.00 18−0.06 0.04 0.05 0.00 −0.07 0.03 −0.06 19 16.00 18.31 17.42 14.23 14.8116.54 12.72 20 8.55 9.67 5.42 3.05 4.07 3.72 3.21 21 21.96 33.03 34.8011.73 18.78 21.00 9.88 22 6.06 6.73 9.55 7.84 7.81 8.35 5.47 23 2.333.00 1.00 1.00 1.00 1.00 1.00 24 13.92 11.00 6.78 8.45 9.15 5.12 16.1325 6.00 8.67 7.67 6.33 7.00 7.00 6.67

TABLE 30 Correlation between the expression level of selected genes ofsome embodiments of the invention in various tissues and the phenotypicperformance under low nitrogen, normal or drought stress conditionsacross Barley accessions (set 1) Gene Exp. Corr. Gene Exp. Corr. Name RP value set Set ID Name R P value set Set ID LYM1010 0.89 1.28E−03 1 30LYM1010 0.86 2.91E−03 1 19 LYM1010 0.71 3.13E−02 1 37 LYM1010 0.932.58E−04 2 11 LYM1010 0.71 3.22E−02 3 3 LYM1010 0.83 5.24E−03 3 22LYM1011 0.72 2.92E−02 1 30 LYM1011 0.84 2.09E−03 5 8 LYM1012 0.753.37E−02 4 33 LYM1012 0.75 3.09E−02 4 21 LYM1013 0.85 7.13E−03 4 21LYM1013 0.74 2.34E−02 2 10 LYM1014 0.76 1.83E−02 1 30 LYM1015 0.791.91E−02 6 5 LYM1015 0.72 2.90E−02 2 1 LYM1015 0.89 1.21E−03 2 13LYM1015 0.72 2.87E−02 3 30 LYM1015 0.80 1.01E−02 3 19 LYM1015 0.761.73E−02 3 37 LYM1017 0.80 1.02E−02 1 20 LYM1017 0.81 8.66E−03 2 10LYM1017 0.71 3.12E−02 2 23 LYM1017 0.72 2.84E−02 3 7 LYM1018 0.724.25E−02 6 33 LYM1018 0.72 4.21E−02 6 18 LYM1018 0.80 9.48E−03 1 7LYM1018 0.75 2.04E−02 1 20 LYM1018 0.71 4.82E−02 4 33 LYM1018 0.831.11E−02 4 11 LYM1018 0.83 9.93E−03 4 9 LYM1018 0.78 2.37E−02 4 4LYM1018 0.79 1.88E−02 4 12 LYM1018 0.87 2.15E−03 2 33 LYM1018 0.781.39E−02 2 12 LYM1018 0.71 3.15E−02 2 13 LYM1018 0.86 3.10E−03 3 7LYM1018 0.78 1.39E−02 3 36 LYM1018 0.87 2.35E−03 3 35 LYM1019 0.761.09E−02 5 25 LYM1020 0.71 3.05E−02 2 10 LYM1020 0.81 7.98E−03 3 20LYM1020 0.73 2.68E−02 3 32 LYM1021 0.88 1.69E−03 1 15 LYM1021 0.971.22E−05 2 26 LYM1021 0.92 5.10E−04 2 17 LYM1021 0.80 9.78E−03 3 30LYM1021 0.73 2.45E−02 3 34 LYM1021 0.79 1.06E−02 3 37 LYM1022 0.811.38E−02 6 5 LYM1022 0.80 1.80E−02 4 33 LYM1022 0.76 3.01E−02 4 21LYM1022 0.74 2.39E−02 2 1 LYM1022 0.72 2.97E−02 2 18 LYM1022 0.751.96E−02 2 17 LYM1022 0.87 2.17E−03 3 36 LYM1022 0.95 8.69E−05 3 35LYM1024 0.73 4.12E−02 6 14 LYM1024 0.89 1.27E−03 1 30 LYM1024 0.752.00E−02 1 37 LYM1024 0.80 1.60E−02 4 14 LYM1024 0.82 6.83E−03 3 20LYM1025 0.87 2.38E−03 1 20 LYM1025 0.77 1.42E−02 3 30 LYM1025 0.809.87E−03 3 19 LYM1025 0.71 3.25E−02 3 34 LYM1025 0.85 3.74E−03 3 37LYM1025 0.70 3.41E−02 3 16 LYM1027 0.72 4.47E−02 6 5 LYM1027 0.743.53E−02 4 21 LYM1027 0.72 4.27E−02 4 29 LYM1027 0.93 8.23E−04 4 12LYM1028 0.74 3.70E−02 4 5 LYM1029 0.90 2.37E−03 6 5 LYM1029 0.809.04E−03 1 35 LYM1029 0.72 3.02E−02 2 18 LYM1030 0.77 2.50E−02 6 5LYM1030 0.87 2.14E−03 2 13 LYM1031 0.74 2.20E−02 2 26 LYM1031 0.732.53E−02 2 17 LYM1032 0.70 3.50E−02 3 34 LYM1033 0.99 1.06E−07 1 30LYM1033 0.83 5.43E−03 1 19 LYM1033 0.81 8.70E−03 1 37 LYM1033 0.853.49E−03 2 11 LYM1034 0.77 2.47E−02 6 5 LYM1034 0.71 3.05E−02 1 30LYM1034 0.74 2.30E−02 1 31 LYM1034 0.76 2.73E−02 4 28 LYM1034 0.742.21E−02 2 28 LYM1034 0.73 2.61E−02 2 18 LYM1034 0.88 1.82E−03 26LYM1034 0.90 8.29E−04 L 17 LYM1034 0.76 1.71E−02 3 32 LYM1035 0.893.08E−03 4 14 LYM1036 0.93 3.52E−04 2 26 LYM1036 0.87 2.31E−03 2 17LYM1036 0.75 1.94E−02 3 25 LYM1036 0.71 3.14E−02 3 32 LYM1037 0.853.55E−03 1 20 LYM1037 0.74 3.42E−02 4 18 LYM1038 0.79 1.97E−02 4 14LYM1038 0.74 3.46E−02 4 13 LYM1038 0.70 3.51E−02 2 1 LYM1038 0.722.86E−02 2 13 LYM1040 0.71 3.27E−02 1 22 LYM1041 0.70 5.20E−02 4 23LYM1042 0.71 4.78E−02 6 21 LYM1043 0.72 2.95E−02 1 35 LYM1043 0.741.44E−02 5 35 LYM1043 0.75 1.98E−02 3 30 LYM1043 0.76 1.84E−02 3 19LYM1043 0.73 2.70E−02 3 37 LYM1044 0.85 7.86E−03 6 26 LYM1044 0.791.97E−02 6 12 LYM1044 0.73 4.01E−02 4 18 LYM1044 0.78 2.17E−02 4 26LYM1044 0.79 1.86E−02 4 17 LYM1044 0.76 2.89E−02 4 13 LYM1044 0.712.19E−02 5 19 LYM1044 0.82 3.47E−03 5 3 LYM1044 0.84 4.64E−03 2 21LYM1046 0.76 2.92E−02 6 21 LYM1047 0.93 2.35E−04 3 30 LYM1047 0.853.43E−03 3 19 LYM1047 0.79 1.08E−02 3 37 LYM1048 0.84 8.63E−03 4 18LYM1048 0.80 9.50E−03 3 31 LYM1049 0.73 2.48E−02 3 7 LYM1051 0.724.46E−02 6 5 LYM1052 0.86 6.30E−03 4 28 LYM1052 0.78 1.33E−02 3 31LYM1053 0.74 3.66E−02 4 14 LYM1054 0.86 2.67E−03 1 30 LYM1054 0.732.52E−02 1 19 LYM1055 0.70 3.49E−02 2 11 LYM1057 0.73 2.61E−02 3 36LYM1057 0.85 3.70E−03 3 35 LYM1058 0.70 3.46E−02 1 30 LYM1058 0.857.49E−03 4 18 LYM1058 0.83 5.31E−03 2 11 LYM1058 0.73 2.60E−02 3 31LYM1059 0.76 2.98E−02 6 18 LYM1059 0.90 4.41E−04 5 35 LYM1059 0.778.58E−03 5 16 LYM1059 0.81 8.73E−03 2 18 LYM1059 0.73 2.60E−02 2 17LYM1059 0.86 3.08E−03 2 13 LYM1060 0.84 9.16E−03 6 1 LYM1060 0.714.92E−02 6 26 LYM1060 0.74 2.21E−02 2 18 LYM1060 0.75 1.97E−02 2 4LYM1060 0.87 2.58E−03 3 30 LYM1060 0.88 1.91E−03 3 19 LYM1060 0.923.89E−04 3 37 LYM1060 0.83 5.10E−03 3 6 LYM1060 0.80 1.01E−02 3 16LYM1061 0.93 3.39E−04 1 16 LYM1061 0.77 1.54E−02 2 26 LYM1061 0.853.69E−03 2 17 LYM1061 0.80 9.96E−03 2 13 LYM1062 0.75 2.12E−02 1 31LYM1062 0.78 1.35E−02 3 31 LYM1063 0.73 2.57E−02 1 34 LYM1063 0.715.07E−02 4 17 LYM1063 0.71 3.08E−02 2 26 LYM1063 0.72 3.01E−02 2 17LYM1064 0.72 4.51E−02 6 33 LYM1064 0.88 3.78E−03 4 18 LYM1064 0.753.37E−02 4 26 LYM1064 0.77 2.44E−02 4 17 LYM1064 0.76 1.00E−02 5 8LYM1066 0.72 4.43E−02 6 18 LYM1066 0.78 1.37E−02 1 30 LYM1066 0.882.00E−03 2 11 LYM1066 0.73 2.54E−02 2 13 LYM1068 0.84 8.60E−03 6 18LYM1068 0.76 2.95E−02 4 2 LYM1069 0.73 3.88E−02 6 17 LYM1069 0.836.05E−03 1 20 LYM1069 0.78 1.29E−02 2 11 LYM1070 0.74 3.62E−02 6 14LYM1070 0.89 2.85E−03 6 5 LYM1070 0.77 1.51E−02 1 31 LYM1070 0.753.31E−02 4 18 LYM1070 0.95 2.98E−04 4 13 LYM1070 0.74 2.16E−02 3 3LYM1070 0.81 7.75E−03 3 8 LYM1071 0.72 4.43E−02 4 28 LYM1071 0.771.43E−02 2 28 LYM1072 0.81 8.16E−03 1 30 LYM1072 0.83 5.72E−03 1 19LYM1072 0.71 3.05E−02 1 37 LYM1073 0.83 5.76E−03 1 20 LYM1073 0.881.68E−03 3 20 LYM1074 0.78 2.18E−02 6 33 LYM1074 0.78 2.32E−02 6 12LYM1074 0.92 4.49E−04 1 20 LYM1074 0.77 8.96E−03 5 8 LYM1074 0.771.47E−02 2 11 LYM1074 0.72 2.92E−02 2 18 LYM1074 0.89 1.42E−03 2 13LYM1075 0.85 3.90E−03 1 30 LYM1075 0.74 3.77E−02 4 28 LYM1075 0.753.11E−02 4 11 LYM1075 0.70 5.19E−02 4 12 LYM1075 0.72 4.27E−02 4 2LYM1075 0.78 1.39E−02 2 21 LYM1075 0.76 1.86E−02 3 7 LYM1075 0.791.13E−02 3 35 LYM1076 0.85 7.97E−03 4 26 LYM1076 0.72 4.39E−02 4 17LYM1076 0.70 2.36E−02 5 34 LYM1234 0.79 1.13E−02 2 28 LYM1234 0.752.01E−02 3 7 LYM1234 0.72 3.00E−02 3 36 LYM1234 0.86 3.19E−03 3 35 Table30. Provided are the correlations (R) between the expression levelsyield improving genes and their homologs in various tissues [Expression(Exp) sets] and the phenotypic performance [yield, biomass, growth rateand/or vigor components (Correlation vector (Corr))] under normal, lownitrogen and drought conditions across barley varieties. P = p value.

TABLE 31 Correlation between the expression level of selected genes ofsome embodiments of the invention in various tissues and the phenotypicperformance under low nitrogen, normal or drought stress conditionsacross Barley accessions (set 2) Gene Exp. Corr. Gene P Exp. Corr. NameR P value set Set ID Name R value set Set ID LYM1010 0.82 4.45E−02 1 11LYM1010 0.76 2.81E−02 3 22 LYM1010 0.70 3.43E−02 6 2 LYM1010 0.897.96E−03 2 17 LYM1010 0.82 2.31E−02 2 11 LYM1010 0.72 4.34E−02 5 10LYM1010 0.81 1.59E−02 5 2 LYM1010 0.85 7.50E−03 5 15 LYM1011 0.701.20E−01 1 25 LYM1011 0.81 1.43E−02 3 20 LYM1011 0.77 2.40E−02 3 23LYM1011 0.85 7.05E−03 5 18 LYM1012 0.74 9.19E−02 1 11 LYM1012 0.724.43E−02 3 12 LYM1012 0.71 5.04E−02 3 15 LYM1012 0.87 1.18E−02 2 11LYM1013 0.82 4.41E−02 1 12 LYM1013 0.71 1.13E−01 1 19 LYM1013 0.796.11E−02 1 21 LYM1014 0.91 1.70E−03 3 12 LYM1014 0.74 5.62E−02 2 11LYM1015 0.70 3.44E−02 4 4 LYM1016 0.81 4.98E−02 1 11 LYM1016 0.802.97E−02 2 22 LYM1016 0.75 5.14E−02 2 13 LYM1016 0.93 8.12E−03 5 8LYM1017 0.79 6.02E−02 1 22 LYM1017 0.73 3.79E−02 3 13 LYM1017 0.812.59E−02 2 22 LYM1017 0.79 3.38E−02 2 9 LYM1017 0.76 4.94E−02 2 13LYM1017 0.80 1.80E−02 5 15 LYM1018 0.91 1.14E−02 1 13 LYM1018 0.805.48E−02 1 2 LYM1018 0.90 1.32E−02 1 15 LYM1018 0.80 1.80E−02 3 24LYM1018 0.80 1.62E−02 3 11 LYM1018 0.76 1.65E−02 6 16 LYM1018 0.941.49E−03 2 10 LYM1018 0.82 2.34E−02 2 2 LYM1018 0.79 2.06E−02 5 24LYM1018 0.79 1.85E−02 5 11 LYM1018 0.89 2.90E−03 5 1 LYM1018 0.843.76E−02 5 8 LYM1018 0.72 3.00E−02 4 18 LYM1019 0.72 1.06E−01 1 4LYM1019 0.71 3.20E−02 6 22 LYM1019 0.84 1.67E−02 2 17 LYM1020 0.731.02E−01 1 7 LYM1020 0.95 3.61E−03 1 12 LYM1020 0.75 8.53E−02 1 19LYM1020 0.78 6.79E−02 1 6 LYM1020 0.86 2.73E−02 1 21 LYM1020 0.713.26E−02 6 2 LYM1020 0.77 4.36E−02 2 12 LYM1020 0.84 8.84E−03 5 19LYM1020 0.80 1.76E−02 5 9 LYM1020 0.74 3.52E−02 5 13 LYM1020 0.715.02E−02 5 15 LYM1021 0.93 6.52E−03 1 12 LYM1021 0.84 3.59E−02 1 19LYM1021 0.86 2.97E−02 1 5 LYM1021 0.81 5.24E−02 1 6 LYM1021 0.796.16E−02 1 21 LYM1021 0.81 2.84E−02 2 7 LYM1021 0.74 5.67E−02 2 5LYM1021 0.80 2.93E−02 2 6 LYM1021 0.85 1.63E−02 2 9 LYM1021 0.726.92E−02 2 18 LYM1022 0.83 4.15E−02 1 12 LYM1022 0.73 9.73E−02 1 19LYM1022 0.72 1.07E−01 1 5 LYM1022 0.75 3.35E−02 3 6 LYM1022 0.831.13E−02 3 21 LYM1022 0.72 6.77E−02 2 20 LYM1022 0.75 5.27E−02 2 12LYM1022 0.77 2.48E−02 5 15 LYM1022 0.71 3.20E−02 4 10 LYM1022 0.891.48E−03 4 12 LYM1022 0.80 9.04E−03 4 2 LYM1022 0.73 2.69E−02 4 15LYM1023 0.73 1.01E−01 1 23 LYM1023 0.91 1.19E−02 1 25 LYM1023 0.739.63E−02 1 18 LYM1023 0.72 4.46E−02 3 20 LYM1024 0.98 4.61E−04 1 11LYM1024 0.75 2.12E−02 6 7 LYM1024 0.84 4.21E−03 6 5 LYM1024 0.853.46E−03 6 6 LYM1024 0.81 8.46E−03 6 21 LYM1024 0.76 4.84E−02 2 1LYM1024 0.81 1.42E−02 5 10 LYM1024 0.78 2.29E−02 5 12 LYM1024 0.801.60E−02 5 2 LYM1025 0.76 8.00E−02 1 17 LYM1025 0.85 3.30E−02 1 22LYM1025 0.73 9.61E−02 1 11 LYM1025 0.72 4.55E−02 3 12 LYM1025 0.753.28E−02 3 22 LYM1025 0.76 2.88E−02 3 2 LYM1025 0.74 3.68E−02 3 15LYM1025 0.82 2.43E−02 6 8 LYM1025 0.87 1.17E−02 2 11 LYM1025 0.724.57E−02 5 18 LYM1025 0.77 1.56E−02 4 12 LYM1026 0.94 4.90E−03 1 12LYM1026 0.90 1.43E−02 1 19 LYM1026 0.71 1.13E−01 1 5 LYM1026 0.796.05E−02 1 6 LYM1026 0.91 1.23E−02 1 21 LYM1026 0.72 4.39E−02 3 21LYM1026 0.71 3.18E−02 6 4 LYM1026 0.71 7.22E−02 2 17 LYM1026 0.783.77E−02 2 11 LYM1026 0.71 4.90E−02 5 18 LYM1026 0.83 3.92E−02 5 8LYM1026 0.84 4.92E−03 4 24 LYM1026 0.91 6.44E−04 4 18 LYM1026 0.761.86E−02 4 4 LYM1027 0.82 4.78E−02 1 13 LYM1027 0.70 1.21E−01 1 2LYM1027 0.77 7.42E−02 1 15 LYM1027 0.83 1.01E−02 3 22 LYM1027 0.717.42E−02 2 1 LYM1027 0.80 1.75E−02 5 10 LYM1027 0.77 2.65E−02 5 12LYM1027 0.78 2.27E−02 5 2 LYM1027 0.91 1.78E−03 5 15 LYM1028 0.862.94E−02 1 11 LYM1028 0.80 3.21E−02 3 14 LYM1028 0.80 2.96E−02 6 14LYM1028 0.82 2.49E−02 2 11 LYM1028 0.94 4.77E−03 5 14 LYM1028 0.753.33E−02 5 5 LYM1028 0.92 3.52E−03 4 14 LYM1029 0.74 2.15E−02 6 19LYM1029 0.76 4.65E−02 2 20 LYM1029 0.81 8.40E−03 4 13 LYM1030 0.723.00E−02 6 17 LYM1030 0.84 3.65E−02 5 14 LYM1030 0.73 2.47E−02 4 20LYM1031 0.76 8.23E−02 1 22 LYM1031 0.86 5.76E−03 5 22 LYM1032 0.945.86E−03 1 17 LYM1032 0.75 3.31E−02 3 13 LYM1032 0.73 3.78E−02 3 2LYM1032 0.98 2.26E−05 3 15 LYM1032 0.79 1.96E−02 5 10 LYM1032 0.811.53E−02 5 12 LYM1032 0.79 1.97E−02 5 2 LYM1032 0.82 1.25E−02 5 15LYM1032 0.86 1.39E−02 4 14 LYM1033 0.74 9.18E−02 1 17 LYM1033 0.734.12E−02 3 12 LYM1033 0.72 4.21E−02 3 22 LYM1034 0.75 8.80E−02 1 7LYM1034 0.88 2.20E−02 1 12 LYM1034 0.77 7.38E−02 1 25 LYM1034 0.758.88E−02 1 6 LYM1034 0.84 3.73E−02 1 21 LYM1034 0.73 3.98E−02 3 20LYM1034 0.79 1.95E−02 3 18 LYM1034 0.79 2.06E−02 5 20 LYM1034 0.893.46E−03 5 18 LYM1034 0.89 1.44E−03 4 18 LYM1035 0.84 3.80E−02 1 23LYM1035 0.74 9.58E−02 1 6 LYM1035 0.73 6.10E−02 3 8 LYM1035 0.761.68E−02 6 20 LYM1035 0.73 2.48E−02 6 23 LYM1035 0.73 6.19E−02 6 8LYM1035 0.86 1.29E−02 2 9 LYM1035 0.98 8.21E−04 5 14 LYM1035 0.714.91E−02 5 15 LYM1035 0.86 1.36E−02 4 14 LYM1036 0.74 9.34E−02 1 10LYM1036 0.75 8.62E−02 1 24 LYM1036 0.94 4.82E−03 1 13 LYM1036 0.961.88E−03 1 2 LYM1036 0.99 8.43E−05 1 15 LYM1036 0.72 1.04E−01 1 1LYM1036 0.85 6.94E−03 3 20 LYM1036 0.79 1.07E−02 6 5 LYM1036 0.781.39E−02 6 6 LYM1036 0.82 7.43E−03 6 21 LYM1036 0.74 3.74E−02 5 18LYM1036 0.75 3.39E−02 5 21 LYM1036 0.77 1.60E−02 4 20 LYM1036 0.781.27E−02 4 18 LYM1037 0.73 1.00E−01 1 17 LYM1037 0.73 3.84E−02 3 22LYM1037 0.71 4.72E−02 3 15 LYM1037 0.85 1.44E−02 2 17 LYM1037 0.762.94E−02 5 17 LYM1038 0.75 3.26E−02 3 22 LYM1038 0.71 3.12E−02 6 16LYM1038 0.81 1.47E−02 5 15 LYM1038 0.78 2.13E−02 5 1 LYM1038 0.853.00E−02 5 8 LYM1040 0.71 1.12E−01 1 13 LYM1040 0.70 1.21E−01 1 11LYM1040 0.92 1.17E−03 3 17 LYM1040 0.81 7.64E−03 6 10 LYM1040 0.793.35E−02 2 23 LYM1040 0.81 2.83E−02 2 15 LYM1040 0.75 3.15E−02 5 5LYM1040 0.74 3.64E−02 5 4 LYM1040 0.80 3.14E−02 4 8 LYM1041 0.733.98E−02 5 18 LYM1041 0.82 6.36E−03 4 18 LYM1042 0.78 6.96E−02 1 12LYM1042 0.72 1.06E−01 1 21 LYM1042 0.89 2.79E−03 3 20 LYM1042 0.742.23E−02 6 17 LYM1042 0.92 1.39E−03 5 20 LYM1043 0.73 3.95E−02 3 20LYM1043 0.73 2.69E−02 6 19 LYM1043 0.92 5.38E−04 4 18 LYM1044 0.834.16E−02 1 23 LYM1044 0.70 1.20E−01 1 25 LYM1044 0.70 1.20E−01 1 6LYM1044 0.82 1.26E−02 3 20 LYM1044 0.95 2.39E−04 3 24 LYM1044 0.736.21E−02 2 21 LYM1044 0.87 2.11E−03 4 20 LYM1044 0.73 2.55E−02 4 23LYM1046 0.86 2.68E−02 1 17 LYM1046 0.79 6.05E−02 1 1 LYM1046 0.865.63E−03 3 2 LYM1046 0.73 3.98E−02 3 1 LYM1047 0.70 1.21E−01 1 18LYM1047 0.94 5.72E−04 3 2 LYM1047 0.80 1.72E−02 3 15 LYM1047 0.848.91E−03 5 12 LYM1048 0.73 1.01E−01 1 17 LYM1048 0.78 6.49E−02 1 1LYM1048 0.71 5.05E−02 3 22 LYM1048 0.73 4.19E−02 3 2 LYM1048 0.752.13E−02 6 15 LYM1048 0.73 6.08E−02 2 1 LYM1048 0.98 8.86E−04 5 8LYM1048 0.72 6.71E−02 4 8 LYM1049 0.78 6.84E−02 1 21 LYM1051 0.777.22E−02 1 11 LYM1052 0.82 4.55E−02 1 12 LYM1052 0.79 6.37E−02 1 21LYM1052 0.97 5.61E−05 3 25 LYM1052 0.82 2.53E−02 2 25 LYM1052 0.822.53E−02 0 18 LYM1052 0.74 2.13E−02 4 18 LYM1053 0.82 1.25E−02 3 22LYM1053 0.83 5.68E−03 6 9 LYM1053 0.77 2.59E−02 5 9 LYM1054 0.777.05E−02 1 1 LYM1054 0.84 8.86E−03 3 22 LYM1054 0.75 3.25E−02 5 10LYM1054 0.80 1.81E−02 5 2 LYM1054 0.74 3.51E−02 5 15 LYM1054 0.721.05E−01 5 8 LYM1055 0.85 3.34E−02 1 17 LYM1055 0.73 4.01E−02 3 12LYM1055 0.77 2.68E−02 3 22 LYM1055 0.88 8.87E−03 2 11 LYM1056 0.834.00E−02 1 25 LYM1056 0.78 6.46E−02 1 18 LYM1056 0.70 5.09E−02 3 22LYM1056 0.78 1.37E−02 6 12 LYM1056 0.78 1.39E−02 6 15 LYM1057 0.862.62E−02 1 17 LYM1057 0.83 1.09E−02 3 24 LYM1057 0.81 1.45E−02 3 4LYM1057 0.76 1.70E−02 6 25 LYM1057 0.71 7.31E−02 2 17 LYM1057 0.841.91E−02 2 11 LYM1057 0.91 1.57E−03 5 7 LYM1057 0.72 4.62E−02 5 19LYM1057 0.88 4.02E−03 5 5 LYM1057 0.92 1.06E−03 5 6 LYM1057 0.856.85E−03 5 21 LYM1057 0.70 3.47E−02 4 11 LYM1058 0.79 5.93E−02 1 17LYM1058 0.71 5.00E−02 3 12 LYM1058 0.71 4.79E−02 3 22 LYM1058 0.714.81E−02 3 2 LYM1059 0.83 4.00E−02 1 25 LYM1059 0.70 1.21E−01 1 18LYM1059 0.81 2.60E−02 6 8 LYM1059 0.72 6.70E−02 2 20 LYM1059 0.862.81E−02 5 8 LYM1059 0.73 2.50E−02 4 20 LYM1060 0.86 2.87E−02 1 12LYM1060 0.77 7.49E−02 1 25 LYM1060 0.85 8.10E−03 5 23 LYM1060 0.874.66E−03 5 25 LYM1060 0.84 4.92E−03 4 20 LYM1060 0.70 3.55E−02 4 23LYM1061 0.91 1.14E−02 1 13 LYM1061 0.78 6.88E−02 1 2 LYM1061 0.891.82E−02 1 15 LYM1061 0.77 2.42E−02 3 20 LYM1061 0.75 3.29E−02 3 21LYM1061 0.71 3.33E−02 4 20 LYM1062 0.89 1.80E−02 1 11 LYM1062 0.774.13E−02 2 1 LYM1063 0.70 1.19E−01 1 20 LYM1063 0.84 3.46E−02 1 12LYM1063 0.81 5.02E−02 1 25 LYM1063 0.82 1.17E−02 3 12 LYM1063 0.717.13E−02 2 23 LYM1063 0.76 4.97E−02 2 12 LYM1063 0.77 4.46E−02 2 25LYM1063 0.78 2.27E−02 5 12 LYM1064 0.70 1.19E−01 1 20 LYM1064 0.795.93E−02 1 12 LYM1064 0.76 2.93E−02 3 20 LYM1064 0.76 2.70E−02 3 23LYM1064 0.78 2.29E−02 3 18 LYM1064 0.81 8.67E−03 6 7 LYM1064 0.751.95E−02 6 6 LYM1064 0.72 6.61E−02 2 25 LYM1064 0.78 3.92E−02 2 18LYM1064 0.73 4.10E−02 5 20 LYM1064 0.85 4.13E−03 4 20 LYM1065 0.721.10E−01 1 20 LYM1065 0.74 3.46E−02 3 24 LYM1066 0.74 9.16E−02 1 20LYM1066 0.91 1.24E−02 1 9 LYM1066 0.90 6.35E−03 2 20 LYM1066 0.862.89E−02 5 14 LYM1068 0.78 6.76E−02 1 22 LYM1068 0.71 3.15E−02 6 20LYM1068 0.76 1.86E−02 6 21 LYM1068 0.81 2.59E−02 2 2 LYM1068 0.832.23E−02 2 15 LYM1068 0.82 1.32E−02 5 10 LYM1068 0.82 1.27E−02 5 12LYM1068 0.76 2.92E−02 5 2 LYM1069 0.75 8.89E−02 1 17 LYM1069 0.734.12E−02 3 12 LYM1069 0.77 2.45E−02 3 22 LYM1069 0.79 1.88E−02 3 2LYM1069 0.75 3.39E−02 3 15 LYM1069 0.70 3.42E−02 4 5 LYM1069 0.761.71E−02 4 9 LYM1070 0.84 3.56E−02 1 13 LYM1070 0.73 9.81E−02 1 2LYM1070 0.81 4.86E−02 1 15 LYM1070 0.71 4.89E−02 3 22 LYM1070 0.753.24E−02 3 25 LYM1070 0.74 2.24E−02 6 6 LYM1070 0.75 5.20E−02 2 13LYM1070 0.81 2.59E−02 2 15 LYM1070 0.77 2.52E−02 5 10 LYM1070 0.753.06E−02 5 2 LYM1070 0.72 4.22E−02 5 15 LYM1070 0.83 3.98E−02 5 8LYM1070 0.85 3.80E−03 4 20 LYM1071 0.78 2.17E−02 3 10 LYM1071 0.782.15E−02 3 12 LYM1071 0.83 1.96E−02 2 22 LYM1071 0.79 3.37E−02 2 9LYM1071 0.77 2.51E−02 5 5 LYM1071 0.73 4.08E−02 5 15 LYM1071 0.861.20E−02 4 14 LYM1071 0.88 1.80E−03 4 22 LYM1071 0.73 2.43E−02 4 15LYM1072 0.79 5.90E−02 1 25 LYM1072 0.80 1.82E−02 5 7 LYM1072 0.705.26E−02 5 25 LYM1072 0.81 1.45E−02 5 6 LYM1072 0.86 3.00E−02 5 8LYM1072 0.74 3.42E−02 5 21 LYM1072 0.82 6.70E−03 4 24 LYM1072 0.781.29E−02 4 11 LYM1072 0.73 2.41E−02 4 4 LYM1073 0.77 7.44E−02 1 23LYM1073 0.74 9.54E−02 1 25 LYM1073 0.76 8.19E−02 1 6 LYM1073 0.921.30E−03 3 20 LYM1073 0.82 1.29E−02 3 24 LYM1073 0.85 1.65E−02 6 8LYM1073 0.79 3.43E−02 2 7 LYM1073 0.82 2.52E−02 2 5 LYM1073 0.896.79E−03 2 6 LYM1073 0.71 7.59E−02 2 18 LYM1073 0.85 1.51E−02 2 21LYM1073 0.76 1.76E−02 4 20 LYM1073 0.77 1.46E−02 4 23 LYM1074 0.796.27E−02 1 25 LYM1074 0.79 3.31E−02 2 17 LYM1074 0.85 3.28E−02 5 14LYM1074 0.73 4.03E−02 5 15 LYM1074 0.73 9.83E−02 5 8 LYM1075 0.891.90E−02 1 22 LYM1075 0.84 3.53E−02 1 11 LYM1075 0.82 1.28E−02 3 22LYM1075 0.71 7.65E−02 6 8 LYM1075 0.70 3.43E−02 6 16 LYM1075 0.848.99E−03 5 13 LYM1075 0.81 1.46E−02 5 11 LYM1076 0.92 1.04E−02 1 11LYM1076 0.72 4.59E−02 3 2 LYM1234 0.79 6.00E−02 1 11 LYM1234 0.705.15E−02 3 20 LYM1234 0.71 4.79E−02 3 23 LYM1234 0.81 1.56E−02 3 21LYM1234 0.73 6.18E−02 2 25 LYM1234 0.77 2.54E−02 5 18 LYM1234 0.726.87E−02 4 8 Table 31. Provided are the correlations (R) between theexpression levels yield improving genes and their homologs in varioustissues [Expression (Exp) sets] and the phenotypic performance [yield,biomass, growth rate and/or vigor components (Correlation vector(Corr))] under normal, low nitrogen and drought conditions across barleyvarieties. P = p value.

Example 7 Production of Brachypodium Transcriptome and High ThroughputCorrelation Analysis Using 60K Brachypodium Oligonucleotide Micro-Array

In order to produce a high throughput correlation analysis comparingbetween plant phenotype and gene expression level, the present inventorsutilized a brachypodium oligonucleotide micro-array, produced by AgilentTechnologies [chem. (dot) agilent (dot) com/Scripts/PDS (dot)asp?1Page=50879]. The array oligonucleotide represents about 60Kbrachypodium genes and transcripts. In order to define correlationsbetween the levels of RNA expression and yield or vigor relatedparameters, various plant characteristics of 24different brachypodiumaccessions were analyzed. Among them, 22 accessions encompassing theobserved variance were selected for RNA expression analysis andcomparative genomic hybridization (CGH) analysis.

The correlation between the RNA levels and the characterized parameterswas analyzed using Pearson correlation test [davidmlane (dot)com/hyperstat/A34739 (dot) html].

Additional correlation analysis was done by comparing plant phenotypeand gene copy number. The correlation between the normalized copy numberhybridization signal and the characterized parameters was analyzed usingPearson correlation test [davidmlane (dot) com/hyperstat/A34739 (dot)html].

Experimental Procedures

Analyzed Brachypodium tissues—two tissues [leaf and spike] were sampledand RNA was extracted as described above. Each micro-array expressioninformation tissue type has received a Set ID as summarized in Table 32below.

TABLE 32 Brachypodium transcriptome expression sets Expression Set SetID Leaf at flowering stage under normal growth conditions 1 Leaf atflowering stage under normal growth conditions 2 spike at floweringstage under normal growth conditions 3 Table 32. From set ID No. 1 thesample was used to extract DNA; from set ID No. 2 and 3 the samples wereused to extract RNA.

Brachypodium yield components and vigor related parameters assessment—24brachypodium accessions were grown in 4-6 repetitive plots (8 plant perplot), in a green house. The growing protocol was as follows:brachypodium seeds were sown in plots and grown under normal condition.Plants were continuously phenotyped during the growth period and atharvest (Table 34-35, below). The image analysis system included apersonal desktop computer (Intel P4 3.0 GHz processor) and a publicdomain program—ImageJ 1.37 (Java based image processing program, whichwas developed at the U.S. National Institutes of Health and freelyavailable on the internet [rsbweb (dot) nih (dot) gov/]. Next, analyzeddata was saved to text files and processed using the JMP statisticalanalysis software (SAS institute).

At the end of the growing period the grains were separated from thespikes and the following parameters were measured using digital imagingsystem and collected:

No. of tillering—all tillers were counted per plant at harvest (mean perplot).

Head number—At the end of the experiment, heads were harvested from eachplot and were counted.

Total Grains weight per plot (gr.)—At the end of the experiment (plant‘Heads’) heads from plots were collected, the heads were threshed andgrains were weighted. In addition, the average grain weight per head wascalculated by dividing the total grain weight by number of total headsper plot (based on plot).

Highest number of spikelets—The highest spikelet number per head wascalculated per plant (mean per plot).

Mean number of spikelets—The mean spikelet number per head wascalculated per plot.

Plant height—Each of the plants was measured for its height usingmeasuring tape. Height was measured from ground level to spike base ofthe longest spike at harvest.

Spikelets weight (gr.)—The biomass and spikes weight of each plot wasseparated, measured per plot.

Average head weight—calculated by dividing spikelets weight with headnumber (gr.).

Harvest Index—harvest index was performed using Formula LXV above.

Spikelets Index—Spikelets index was performed using Formula XXXI.

Percent Number of heads with spikelets—The number of heads with morethan one spikelet per plant were counted and the percent from all headsper plant was calculated.

Total dry mater per plot—Calculated as Vegetative portion above groundplus all the spikelet dry weight per plot.

1000 grain weight—At the end of the experiment all grains from all plotswere collected and weighted and the weight of 1000 were calculated.

The following parameters were collected using digital imaging system:

At the end of the growing period the grains were separated from thespikes and the following parameters were measured and collected:

(i) Average Grain Area (cm²)—A sample of ˜200 grains was weighted,photographed and images were processed using the below described imageprocessing system. The grain area was measured from those images and wasdivided by the number of grains.

(ii) Average Grain Length, perimeter and width (cm)—A sample of ˜200grains was weighted, photographed and images were processed using thebelow described image processing system. The sum of grain lengths andwidth (longest axis) was measured from those images and was divided bythe number of grains.

The image processing system was used, which consists of a personaldesktop computer (Intel P4 3.0 GHz processor) and a public domainprogram—ImageJ 1.37, Java based image processing software, which wasdeveloped at the U.S. National Institutes rsbweb (dot) nih (dot) gov/.Images were captured in resolution of 10Mega Pixels (3888×2592 pixels)and stored in allow compression JPEG (Joint Photographic Experts Groupstandard) format. Next, image processing output data for seed area andseed length was saved to text files and analyzed using the JMPstatistical analysis software (SAS institute).

TABLE 33 Brachypodium correlated parameters (vectors) Corre- lationCorrelated parameter with ID % Num of heads with spikelets 1 1000 grainweight (g) 2 Average head weight (g) 3 Grain Perimeter (cm) 4 Grain area(cm²) 5 Grain length (cm) 6 Grain width (cm) 7 Grains weight per plant(g) 8 Grains weight per plot (g) 9 Harvest index (value) 10 Heads perplant (number) 11 Heads per plot (number) 12 Highest num of spikeletsper plot 13 Mean num of spikelets per plot 14 Num of heads withspikelets per plant 15 Plant height (cm) 17 Plant Vegetative DW (g) 16Plants num 18 Spikelets DW per plant (g) 19 Spikelets weight (g) 20Spikes index (g) 21 Tillering (number) 22 Total dry mater per plant (g)23 Total dry mater per plot (g) 24 Vegetative DW (g) 25 Table 33.Provided are the Brachypodium correlated parameters. “num” = number.

Experimental Results

24 different parameters as described above. The average for each of themeasured parameter was calculated using the JMP software and values aresummarized in Tables 34-35 below. Subsequent correlation analysisbetween the various transcriptome sets and the average parameters (Table36) was conducted. Follow, results were integrated to the database.

TABLE 34 Measured parameters IDs in Brachypodium of correlationaccessions under normal conditions Ecotype/ Line- Line- Line- TreatmentLine-1 2 3 Line-4 Line-5 Line-6 Line-7 8 1 27.61 35.33 21.67 52.40 20.8447.73 17.55 16.51 2 3.75 3.78 3.35 3.70 3.90 4.87 4.82 4.76 3 0.0570.044 0.049 0.087 0.040 0.087 0.060 0.055 4 1.67 1.62 1.62 1.65 1.601.90 1.80 1.82 5 0.102 0.096 0.094 0.088 0.086 0.113 0.105 0.111 6 0.7330.719 0.717 0.750 0.724 0.871 0.794 0.789 7 0.178 0.168 0.167 0.1490.151 0.165 0.168 0.180 8 0.14 0.06 0.08 0.35 0.27 0.44 0.32 0.07 9 1.050.44 0.61 2.58 2.03 3.40 2.58 0.39 10 0.13 0.14 0.15 0.21 0.17 0.18 0.150.11 11 16.29 7.08 6.59 16.11 21.40 17.05 25.88 8.02 12 121.75 56.6052.75 123.50 156.83 135.0 207.00 48.60 13 3.00 2.60 3.00 2.83 2.33 4.502.60 2.00 14 2.10 2.10 1.72 2.17 1.85 2.85 1.93 1.56 15 5.27 2.50 2.069.44 5.02 7.72 4.90 1.87 16 0.42 0.12 0.13 0.82 0.67 1.05 0.87 0.31 1731.65 23.44 22.75 45.35 29.41 46.74 38.39 29.15 18 7.50 8.00 8.00 7.507.33 7.88 8.00 6.40 19 0.96 0.31 0.33 1.46 0.96 1.42 1.56 0.45 20 7.182.50 2.68 11.31 7.16 11.05 12.44 2.66 21 0.71 0.72 0.73 0.68 0.60 0.570.65 0.60 22 16.84 7.20 7.00 16.99 23.61 18.25 27.20 8.60 23 1.38 0.430.47 2.28 1.63 2.47 2.43 0.76 24 10.26 3.45 3.74 17.78 12.29 19.27 19.404.47 25 3.08 0.95 1.06 6.47 5.13 8.23 6.96 1.81 Table 34. CorrelationIDs: 1, 2, 3, 4, 5, . . . etc. refer to those described in Table 33above [Brachypodium correlated parameters (vectors)].

TABLE 35 Correlation IDs: 1, 2, 3, 4, 5, . . . etc. refer to thosedescribed in Table 33 above [Brachypodium correlated parameters(vectors)]. Measured parameters of correlation IDs in brachypodiumaccessions under normal conditions Ecotype/ Line- Line- Line- Line-Line- Line- Line- Treatment 9 10 11 12 13 14 15 1 5.42 15.42 14.00 6.404.51 15.52 20.34 2 5.54 4.98 4.88 4.83 5.54 4.73 5.24 3 0.040 0.0560.075 0.048 0.042 0.048 0.053 4 1.82 1.83 1.69 1.74 1.93 1.69 1.91 50.105 0.111 0.094 0.102 0.110 0.100 0.124 6 0.833 0.824 0.740 0.7810.896 0.748 0.857 7 0.161 0.172 0.162 0.166 0.155 0.169 0.185 8 0.140.14 0.26 0.14 0.11 0.39 0.14 9 1.11 1.07 1.96 1.09 0.84 3.07 1.09 100.20 0.16 0.20 0.14 0.26 0.22 0.09 11 10.48 9.09 11.63 14.13 5.88 23.7516.06 12 82.40 70.13 83.40 110.33 47.00 185.50 125.00 13 2.00 2.25 2.201.83 2.00 2.50 2.40 14 1.38 1.65 1.69 1.43 1.25 1.76 1.83 15 0.71 1.942.08 1.08 0.35 4.98 3.70 16 0.32 0.32 0.38 0.39 0.13 0.87 0.69 17 34.3628.65 31.95 28.88 24.74 37.30 45.09 18 7.80 7.75 7.20 7.83 8.00 7.758.00 19 0.44 0.56 0.88 0.67 0.26 1.14 0.83 20 3.45 4.29 6.42 5.29 2.048.89 6.65 21 0.58 0.66 0.71 0.64 0.66 0.59 0.54 22 10.67 9.38 11.9714.58 6.35 25.50 16.56 23 0.76 0.88 1.25 1.06 0.38 2.01 1.53 24 6.006.78 9.12 8.34 3.04 15.79 12.20 25 2.55 2.48 2.69 3.05 1.00 6.89 5.55

TABLE 36 Correlation IDs: 1, 2, 3, 4, 5, . . . etc. refer to thosedescribed in Table 33 above [Brachypodium correlated parameters(vectors)]. Measured parameters of correlation IDs in brachypodiumaccessions under normal conditions Ecotype/ Line- Line- Line- Line-Line- Line- Line- Treatment 16 17 18 19 20 21 22 1 8.11 53.21 55.4147.81 42.81 59.01 34.92 2 4.96 4.00 3.84 4.26 5.99 3.76 4.34 3 0.0570.104 0.078 0.079 0.082 0.090 0.064 4 1.71 1.81 1.68 1.75 1.87 1.68 1.665 0.100 0.096 0.101 0.090 0.117 0.092 0.091 6 0.744 0.839 0.748 0.8020.842 0.764 0.736 7 0.171 0.146 0.171 0.143 0.177 0.152 0.157 8 0.130.37 0.08 0.49 0.31 0.30 0.20 9 1.07 2.99 0.50 3.52 2.41 1.92 1.47 100.18 0.09 0.07 0.16 0.18 0.09 0.11 11 9.74 22.19 11.89 24.32 13.25 25.5419.22 12 80.75 177.50 81.50 172.80 98.60 177.00 143.17 13 2.00 3.50 3.503.80 2.80 3.17 2.83 14 1.42 2.71 2.41 2.61 2.12 2.79 2.15 15 0.89 12.587.59 12.13 6.35 15.36 7.15 16 0.34 1.72 0.44 1.32 0.48 1.73 0.63 1722.39 55.04 31.40 45.34 40.20 58.82 39.18 18 8.25 8.00 6.50 7.00 7.606.83 7.33 19 0.59 2.27 0.92 1.91 1.09 2.25 1.26 20 4.92 18.15 6.25 13.498.35 15.55 9.42 21 0.68 0.56 0.69 0.59 0.70 0.57 0.66 22 10.53 27.1512.38 26.30 13.56 29.09 20.79 23 0.93 3.99 1.36 3.23 1.57 3.98 1.89 247.76 31.94 9.21 22.78 12.04 27.67 14.14 25 2.84 13.80 2.96 9.28 3.7012.12 4.72

TABLE 37 Provided are the correlations (R) between the expression levelsyield improving genes and their homologs in various tissues [Expression(Exp) sets] and the phenotypic performance [yield, biomass, growth rateand/or vigor components (Correlation vector (Corr))] under normalconditions across brachypodium varieties P = p value. Correlationbetween the expression level of selected genes of some embodiments ofthe invention in various tissues and the phenotypic performance undernormal conditions across brachypodium varieties Gene P Exp. Corr. SetGene P Exp. Corr. Set Name R value set ID Name R value set ID LYM10990.85 5.21E−04 3 10 LYM1082 0.72 8.94E−03 3 7 LYM1082 0.73 7.32E−03 3 20LYM1084 0.71 9.89E−03 3 23 LYM1084 0.83 8.85E−04 3 14 LYM1084 0.711.01E−02 3 22 LYM1084 0.80 1.76E−03 3 20 LYM1084 0.72 7.84E−03 3 1LYM1084 0.88 1.85E−04 3 8 LYM1084 0.84 6.71E−04 3 9 LYM1084 0.837.36E−04 3 13 LYM1084 0.73 7.51E−03 3 24 LYM1084 0.72 8.26E−03 3 3LYM1084 0.78 2.89E−03 3 19 LYM1084 0.71 1.03E−02 3 15 LYM1084 0.721.89E−02 2 22 LYM1092 0.71 1.48E−02 1 22 LYM1094 0.74 1.39E−02 2 22LYM1096 0.72 1.32E−02 1 22 LYM1097 0.80 3.42E−03 1 22

Example 8 Production of Foxtail Millet Transcriptome and High ThroughputCorrelation Analysis Using 60K Foxtaill Millet OligonucleotideMicro-Array

In order to produce a high throughput correlation analysis comparingbetween plant phenotype and gene expression level, the present inventorsutilized a foxtail millet oligonucleotide micro-array, produced byAgilent Technologies [chem. (dot) agilent (dot) com/Scripts/PDS (dot)asp?1Page=50879]. The array oligonucleotide represents about 60K foxtailmillet genes and transcripts. In order to define correlations betweenthe levels of RNA expression and yield or vigor related parameters,various plant characteristics of 15 different foxtail millet accessionswere analyzed. Among them, 11 accessions encompassing the observedvariance were selected for RNA expression analysis. The correlationbetween the RNA levels and the characterized parameters was analyzedusing Pearson correlation test [davidmlane (dot) com/hyperstat/A34739(dot) html].

Experimental Procedures

Analyzed Foxtail millet tissues—three tissues at different developmentalstages [leaf, flower, and stem], representing different plantcharacteristics, were sampled and RNA was extracted as described above.Each micro-array expression information tissue type has received a SetID as summarized in Table 38 below.

TABLE 38 Foxtail millet transcriptome expression sets Expression Set SetID Grain, grain filling stage, normal 1 Leaf, grain fitting stage,normal 2 Stem, grain filling stage, normal 3 Flower, flowering stage,normal 4 Leaf, flowering stage, normal 5 Table 38.

Foxtaill millet yield components and vigor related parametersassessment—14 Foxtail millet accessions in 5 repetitive plots, in thefield. Foxtaill millet seeds were sown in soil and grown under normalcondition in the field. Plants were continuously phenotyped during thegrowth period and at harvest (Tables 40-41, below). The image analysissystem included a personal desktop computer (Intel P4 3.0 GHz processor)and a public domain program—ImageJ 1.37 (Java based image processingprogram, which was developed at the U.S. National Institutes of Healthand freely available on the internet [rsbweb (dot) nih (dot) gov/].Next, analyzed data was saved to text files and processed using the JMPstatistical analysis software (SAS institute).

The following parameters were collected using digital imaging system:

At the end of the growing period the grains were separated from thePlant ‘Head’ and the following parameters were measured and collected:

(i) Average Grain Area (cm²)—A sample of ˜200 grains was weighted,photographed and images were processed using the below described imageprocessing system. The grain area was measured from those images and wasdivided by the number of grains.

(ii) Average Grain Length and width (cm)—A sample of ˜200 grains wasweighted, photographed and images were processed using the belowdescribed image processing system. The sum of grain lengths and width(longest axis) was measured from those images and was divided by thenumber of grains.

At the end of the growing period 14 ‘Heads’ were photographed and imageswere processed using the below described image processing system.

(i) Head Average Area (cm²)—The ‘Head’ area was measured from thoseimages and was divided by the number of ‘Heads’.

(ii) Head Average Length (mm)—The ‘Head’ length (longest axis) wasmeasured from those images and was divided by the number of ‘Heads’.

The image processing system was used, which consists of a personaldesktop computer (Intel P4 3.0 GHz processor) and a public domainprogram—ImageJ 1.37, Java based image processing software, which wasdeveloped at the U.S. National Institutes of Health and is freelyavailable on the internet at rsbweb (dot) nih (dot) gov/. Images werecaptured in resolution of 10 Mega Pixels (3888×2592 pixels) and storedin a low compression JPEG (Joint Photographic Experts Group standard)format. Next, image processing output data for seed area and seed lengthwas saved to text files and analyzed using the JMP statistical analysissoftware (SAS institute).

Additional parameters were collected either by sampling 5 plants perplot or by measuring the parameter across all the plants within theplot.

Total Grain Weight (gr.)—At the end of the experiment (plant ‘Heads’)heads from plots were collected, the heads were threshed and grains wereweighted. In addition, the average grain weight per head was calculatedby dividing the total grain weight by number of total heads per plot(based on plot).

Head weight and head number—At the end of the experiment, heads wereharvested from each plot and were counted and weighted (kg.).

Biomass at harvest—At the end of the experiment the vegetative materialfrom plots was weighted.

Dry weight=total weight of the vegetative portion above ground(excluding roots) after drying at 70° C. in oven for 48 hours atharvest.

Total dry mater per plot—Calculated as Vegetative portion above groundplus all the heads dry weight per plot.

Num days to anthesis—Calculated as the number of days from sowing till50% of the plot arrive anthesis.

TABLE 39 Foxtail millet correlated parameters (vectors) Corre- lation-Correlated parameter with ID 1000 grain weight (g) 1 Biomass at harvestat one meter (kg) 2 Grain area (cm²) 3 Grain length (cm) 4 Grain width(cm) 5 Grains yield per Head (plot) (g) 6 Head Area (cm²) 7 Head length(cm) 8 Heads num 9 Num days to Anthesis 10 Total Grains yield (g) 11Total dry matter at one meter (kg) 12 Total heads weight (kg) 13 Table39. Provided are the foxtail millet correlated parameters.

Experimental Results

14 different foxtail millet accessions were grown and characterized fordifferent parameters as described above. The average for each of themeasured parameter was calculated using the JMP software and values aresummarized in Tables 40-41 below. Subsequent correlation analysisbetween the various transcriptome sets and the average parameters (Table42) was conducted. Follow, results were integrated to the database.

TABLE 40 Correlation IDs: 1, 2, 3, 4, 5, . . . etc. refer to thosedescribed in Table 39 above [Foxtail millet correlated parameters(vectors)]. Measured parameters of correlation IDs in foxtail milletaccessions under normal conditions Treatment Line-1 Line-2 Line-3 Line-4Line-5 Line-6 Line-7 1 2.46 3.42 2.61 2.36 2.41 2.65 2.18 2 2.40 3.993.17 3.58 3.60 3.06 4.04 3 0.032 0.037 0.033 0.032 0.032 0.034 0.029 40.240 0.242 0.249 0.253 0.256 0.252 0.231 5 0.172 0.194 0.167 0.1590.160 0.170 0.162 6 3.40 7.29 1.75 1.30 1.57 0.69 2.10 7 37.83 57.8719.59 17.10 19.76 9.42 22.92 8 23.13 24.25 17.56 14.79 15.38 8.56 16.089 427.60 149.20 867.00 1204.00 1146.40 2132.00 752.20 10 34.00 41.0045.00 41.00 41.00 30.00 38.00 11 1449.63 1067.88 1534.92 1567.20 1794.801476.11 1582.57 12 0.70 0.85 0.96 0.92 0.90 0.48 0.92 13 3.81 5.95 6.205.64 6.27 6.07 6.32

TABLE 41 Correlation Ds: 1, 2, 3, 4, 5, . . . etc. refer to thosedescribed in Table 39 above [Foxtail millet correlated parameters(vectors)]. Measured parameters of correlation IDs in foxtail milletaccessions under normal conditions Ecotype/ Treatment Line-8 Line-9Line-10 Line-11 Line-12 Line-13 Line-14 1 1.80 2.69 1.65 3.17 2.60 3.182.26 2 1.15 3.20 3.90 3.58 3.68 2.94 1.48 3 0.024 0.032 0.025 0.0370.033 0.039 0.030 4 0.196 0.221 0.199 0.262 0.250 0.269 0.244 5 0.1550.184 0.157 0.181 0.169 0.183 0.158 6 3.34 11.46 7.17 4.35 2.26 0.441.31 7 40.89 45.29 49.34 27.69 24.18 7.13 14.69 8 21.88 20.41 23.3220.87 17.98 6.35 9.78 9 394.20 186.60 131.80 434.20 646.40 2797.80994.60 10 30.00 38.00 51.00 44.00 51.00 31.00 27.00 11 1317.88 2131.60937.93 1880.21 1427.12 1216.24 1296.69 12 0.45 0.59 1.00 0.91 1.03 0.620.46 13 2.82 7.25 5.24 6.58 5.85 5.62 2.73

TABLE 42 Provided are the correlations (R) between the expression levelsyield improving genes and their homologs in various tissues [Expression(Exp) sets] and the phenotypic performance [yield, biomass, growth rateand/or vigor components (Correlation vector (Cor))] under normal, lownitrogen and drought conditions across foxtail millet varieties. P = pvalue. Correlation between the expression level of selected genes ofsome embodiments of the invention in various tissues and the phenotypicperformance under normal conditions across foxtail millet varieties GeneP Exp. Corr. Set Gene P Exp. Corr. Set Name R value set ID Name R valueset ID LYM1100 0.80 5.28E−03 2 11 LYM1100 0.76 1.10E−02 2 8 LYM1100 0.787.55E−03 2 6 LYM1100 0.83 2.76E−03 2 7 LYM1100 0.73 3.85E−02 3 11LYM1100 0.97 5.97E−05 3 6 LYM1100 0.88 4.43E−03 3 7 LYM1100 0.815.30E−02 1 13 LYM1100 0.84 3.66E−02 1 6 LYM1101 0.89 5.25E−04 2 13LYM1101 0.78 8.25E−03 2 2 LYM1101 0.70 1.19E−01 1 13 LYM1101 0.739.65E−02 1 6 LYM1102 0.78 7.44E−03 2 1 LYM1102 0.72 1.96E−02 2 3 LYM11020.71 4.82E−02 3 1 LYM1102 0.77 2.56E−02 3 3 LYM1102 0.84 9.42E−03 3 9LYM1102 0.81 5.29E−02 1 13 LYM1102 0.82 4.46E−02 1 6 LYM1103 0.721.06E−01 1 1 LYM1103 0.80 5.43E−02 1 8 LYM1103 0.75 8.51E−02 1 3 LYM11030.70 1.21E−01 1 7 LYM1104 0.71 4.81E−02 3 11 LYM1104 0.89 1.62E−02 1 10LYM1104 0.71 1.17E−01 1 2 LYM1106 0.74 1.52E−02 2 5 LYM1107 0.833.96E−02 1 8 LYM1107 0.91 1.26E−02 1 6 LYM1107 0.85 3.06E−02 1 5 LYM11070.87 2.34E−02 1 7 LYM1108 0.72 1.10E−01 1 12 LYM1108 0.90 1.57E−02 1 4LYM1108 0.83 4.18E−02 1 9 LYM1109 0.76 1.10E−02 2 13 LYM1109 0.779.26E−03 2 11 LYM1109 0.81 4.40E−03 2 10 LYM1109 0.79 6.77E−03 2 2LYM1109 0.73 1.58E−02 2 6 LYM1109 0.84 3.63E−02 1 1 LYM1109 0.786.47E−02 1 3 LYM1109 0.81 5.11E−02 1 2 LYM1100 0.90 2.18E−03 3 6 LYM11100.75 3.06E−02 3 7 LYM1110 0.88 2.15E−07 1 8 LYM1110 0.73 9.88E−02 1 6LYM1110 0.77 7.47E−02 1 5 LYM1110 0.83 4.10E−02 1 7 LYM1111 0.862.82E−02 1 8 LYM1111 0.75 8.83E−02 1 7 LYM1112 0.80 1.78E−02 3 11LYM1112 0.71 1.10E−01 1 1 LYM1112 0.83 4.14E−02 1 4 LYM1112 0.853.07E−02 1 3 LYM1113 0.86 6.15E−03 3 12 LYM1113 0.72 4.38E−02 3 8LYM1113 0.82 1.19E−02 3 10 LYM1113 0.72 1.06E−01 1 1 LYM1113 0.749.26E−02 1 10 LYM1114 0.96 2.16E−03 1 11 LYM1115 0.76 8.21E−02 1 8LYM1115 0.84 3.65E−02 1 6 LYM1115 0.83 3.88E−02 1 5 LYM1115 0.853.26E−02 1 7 LYM1116 0.73 9.93E−02 1 1 LYM1116 0.74 9.46E−02 1 8 LYM11160.95 4.07E−03 1 6 LYM1116 0.94 5.84E−03 1 5 LYM1116 0.91 1.29E−02 1 7LYM1117 0.82 1.25E−02 3 11 LYM1117 0.81 1.39E−02 3 8 LYM1117 0.848.67E−03 3 6 LYM1117 0.93 7.48E−04 3 7 LYM1117 0.73 9.99E−02 1 13LYM1118 0.74 3.76E−02 3 13 LYM1118 0.85 7.54E−03 3 11 LYM1118 0.743.51E−02 3 6 LYM1118 0.72 4.46E−02 3 7 LYM1118 0.77 7.28E−02 1 11LYM1119 0.89 1.87E−02 1 13 LYM1119 0.82 4.75E−02 1 11 LYM1119 0.805.85E−02 1 6 LYM1120 0.86 2.91E−02 1 8 LYM1120 0.71 1.11E−01 1 5 LYM11200.79 6.34E−02 1 7 LYM1121 0.76 8.19E−02 1 1 LYM1121 0.95 3.38E−03 1 8LYM1121 0.87 2.49E−02 1 5 LYM1121 0.92 8.65E−03 1 7 LYM1122 0.902.05E−03 3 9 LYM1122 0.90 1.37E−02 1 1 LYM1122 0.76 8.23E−02 1 4 LYM11220.92 9.93E−03 1 8 LYM1122 0.82 4.80E−02 1 3 LYM1122 0.71 1.15E−01 1 9LYM1122 0.99 2.89E−04 1 6 LYM1122 0.96 1.85E−03 1 5 LYM1122 0.984.28E−04 1 7 LYM1122 0.70 5.22E−02 3 4 LYM1123 0.72 4.29E−02 3 3 LYM11230.92 1.25E−03 3 9 LYM1123 0.88 2.19E−02 1 6 LYM1123 0.84 3.43E−02 1 5LYM1123 0.81 4.82E−02 1 7 LYM1124 0.74 9.51E−02 1 6 LYM1125 0.721.79E−02 2 12 LYM1125 0.88 3.76E−03 3 12 LYM1125 0.89 3.06E−03 3 10LYM1125 0.88 2.15E−02 1 12 LYM1126 0.91 1.13E−02 1 13 LYM1126 0.777.42E−02 1 11 LYM1126 0.83 3.98E−02 1 10 LYM1126 0.73 1.01E−01 1 3LYM1127 0.71 2.08E−02 2 5 LYM1127 0.93 6.68E−04 3 6 LYM1127 0.801.64E−02 3 7 LYM1128 0.83 3.96E−02 1 13 LYM1128 0.89 1.73E−02 1 1LYM1128 0.76 7.89E−02 1 11 LYM1128 0.89 1.88E−02 1 3 LYM1123 0.721.95E−02 5 1 LYM1100 0.85 1.69E−03 5 11 LYM1101 0.80 3.31E−03 4 12LYM1101 0.76 6.46E−03 4 10 LYM1102 0.75 1.18E−02 5 11 LYM1103 0.803.40E−03 4 5 LYM1104 0.76 6.31E−03 4 10 LYM1108 0.79 3.65E−03 4 1LYM1108 0.78 4.23E−03 4 3 LYM1110 0.75 8.36E−03 4 12 LYM1110 0.851.05E−03 4 10 LYM1116 0.71 2.07E−02 5 11 LYM1118 0.86 1.23E−03 5 11LYM1120 0.85 1.86E−03 5 11

Example 9 Production of Soybean (Glycine Max) Transcriptome and HighThroughput Correlation Analysis with Yield Parameters Using 44K B.Soybean Oligonucleotide Micro-Arrays

In order to produce a high throughput correlation analysis, the presentinventors utilized a Soybean oligonucleotide micro-array, produced byAgilent Technologies [chem. (dot) agilent (dot) com/Scripts/PDS (dot)asp?1Page=50879]. The array oligonucleotide represents about 42,000Soybean genes and transcripts. In order to define correlations betweenthe levels of RNA expression with yield components or plant architecturerelated parameters or plant vigor related parameters, various plantcharacteristics of 29 different Glycine max varieties were analyzed and12 varieties were further used for RNA expression analysis. Thecorrelation between the RNA levels and the characterized parameters wasanalyzed using Pearson correlation test.

Correlation of Glycine max Genes' Expression Levels with PhenotypicCharacteristics Across Ecotype

Experimental Procedures

29 Soybean varieties were grown in three repetitive plots, in field.Briefly, the growing protocol was as follows: Soybean seeds were sown insoil and grown under normal conditions until harvest. In order to definecorrelations between the levels of RNA expression with yield componentsor plant architecture related parameters or vigor related parameters, 12different Soybean varieties (out of 29 varieties) were analyzed and usedfor gene expression analyses. Analysis was performed at twopre-determined time periods: at pod set (when the soybean pods areformed) and at harvest time (when the soybean pods are ready forharvest, with mature seeds).

TABLE 43 Soybean transcriptome expression sets Expression Set Set IDApical meristem at vegetative stage under normal 1 growth condition Leafat vegetative stage under normal growth condition 2 Leaf at floweringstage under normal growth condition 3 Leaf at pod setting stage undernormal growth condition 4 Root at vegetative stage under normal growthcondition 5 Root at flowering stage under normal growth condition 6 Rootat pod setting stage under normal growth condition 7 Stem at vegetativestage under normal growth condition 8 Stem at pod setting stage undernormal growth condition 9 Flower bud at flowering stage under normalgrowth 10 condition Pod (R3-R4) at pod setting stage under normal growth11 condition Table 43.

RNA extraction—All 12 selected Soybean varieties were sample pertreatment. Plant tissues [leaf, root. Stem, pod, apical meristem, flowerbuds] growing under normal conditions were sampled and RNA was extractedas described above. The collected data parameters were as follows:

Main branch base diameter [mm] at pod set—the diameter of the base ofthe main branch (based diameter) average of three plants per plot.

Fresh weight [gr./plant] at pod set—total weight of the vegetativeportion above ground (excluding roots) before drying at pod set, averageof three plants per plot.

Dry weight [gr./plant] at pod set—total weight of the vegetative portionabove ground (excluding roots) after drying at 70° C. in oven for 48hours at pod set, average of three plants per plot.

Total number of nodes with pods on lateral branches[value/plant]—counting of nodes which contain pods in lateral branchesat pod set, average of three plants per plot.

Number of lateral branches at pod set [value/plant]—counting number oflateral branches at pod set, average of three plants per plot.

Total weight of lateral branches at pod set [gr./plant]—weight of alllateral branches at pod set, average of three plants per plot.

Total weight of pods on main stem at pod set [gr./plant]—weight of allpods on main stem at pod set, average of three plants per plot.

Total number of nodes on main stem [value/plant]—count of number ofnodes on main stem starting from first node above ground, average ofthree plants per plot.

Total number of pods with 1 seed on lateral branches at pod set[value/plant]—count of the number of pods containing 1 seed in alllateral branches at pod set, average of three plants per plot.

Total number of pods with 2 seeds on lateral branches at pod set[value/plant]—count of the number of pods containing 2 seeds in alllateral branches at pod set, average of three plants per plot.

Total number of pods with 3 seeds on lateral branches at pod set[value/plant]—count of the number of pods containing 3 seeds in alllateral branches at pod set, average of three plants per plot.

Total number of pods with 4 seeds on lateral branches at pod set[value/plant]—count of the number of pods containing 4 seeds in alllateral branches at pod set, average of three plants per plot.

Total number of pods with 1 seed on main stem at pod set[value/plant]—count of the number of pods containing 1 seed in main stemat pod set, average of three plants per plot.

Total number of pods with 2 seeds on main stem at pod set[value/plant]—count of the number of pods containing 2 seeds in mainstem at pod set, average of three plants per plot.

Total number of pods with 3 seeds on main stem at pod set[value/plant]—count of the number of pods containing 3 seeds in mainstem at pod set, average of three plants per plot.

Total number of pods with 4 seeds on main stem at pod set[value/plant]—count of the number of pods containing 4 seeds in mainstem at pod set, average of three plants per plot.

Total number of seeds per plant at pod set [value/plant]—count of numberof seeds in lateral branches and main stem at pod set, average of threeplants per plot.

Total number of seeds on lateral branches at pod set [value/plant]—countof total number of seeds on lateral branches at pod set, average ofthree plants per plot.

Total number of seeds on main stem at pod set [value/plant]—count oftotal number of seeds on main stem at pod set, average of three plantsper plot.

Plant height at pod set [cm/plant]—total length from above ground tillthe tip of the main stem at pod set, average of three plants per plot.

Plant height at harvest[cm/plant]—total length from above ground tillthe tip of the main stem at harvest, average of three plants per plot.

Total weight of pods on lateral branches at pod set [gr./plant]—weightof all pods on lateral branches at pod set, average of three plants perplot.

Ratio of the number of pods per node on main stem at pod set—wasperformed using Formula XXIII above, average of three plants per plot.

Ratio of total number of seeds in main stem to number of seeds onlateral branches—was performed using Formula XXIV above, average ofthree plants per plot.

Total weight of pods per plant at pod set [gr./plant]—weight of all podson lateral branches and main stem at pod set, average of three plantsper plot.

Days till 50% flowering [days]—number of days till 50% flowering foreach plot.

Days till 100% flowering [days]—number of days till 100% flowering foreach plot.

Maturity [days]—measure as 95% of the pods in a plot have ripened(turned 100% brown). Delayed leaf drop and green stems are notconsidered in assigning maturity. Tests are observed 3 days per week,every other day, for maturity. The maturity date is the date that 95% ofthe pods have reached final color. Maturity is expressed in days afterAugust 31 [according to the accepted definition of maturity in USA,Descriptor list for SOYBEAN, ars-grin (dot)gov/cgi-bin/npgs/htmldesclist (dot) pl?51].

Seed quality [ranked 1-5]—measure at harvest; a visual estimate based onseveral hundred seeds. Parameter is rated according to the followingscores considering the amount and degree of wrinkling, defective coat(cracks), greenishness, and moldy or other pigment. Rating is 1—verygood, 2—good, 3—fair, 4—poor, 5—very poor.

Lodging franked 1-51—was rated at maturity per plot according to thefollowing scores: 1-most plants in a plot are erected; 2-all plantsleaning slightly or a few plants down; 3-all plants leaning moderately,or 25%-50% down; 4-all plants leaning considerably, or 50%-80% down;5-most plants down. Note: intermediate score such as 1.5 are acceptable.

Seed size [gr.]—weight of 1000 seeds per plot normalized to 13%moisture, measure at harvest.

Total weight of seeds per plant [gr./plant]—calculated at harvest (per 2inner rows of a trimmed plot) as weight in grams of cleaned seedsadjusted to 13% moisture and divided by the total number of plants intwo inner rows of a trimmed plot.

Yield at harvest [bushels/hectare]—calculated at harvest (per 2 innerrows of a trimmed plot) as weight in grams of cleaned seeds, adjusted to13% moisture, and then expressed as bushels per acre.

Average lateral branch seeds per pod [number]—Calculate Num of Seeds onlateral branches—at pod set and divide by the Number of Total number ofpods with seeds on lateral branches—at pod set.

Average main stem seeds per pod [number]—Calculate Total Number of Seedson main stem at pod set and divide by the Number of Total number of podswith seeds on main stem at pod setting.

Main stem average internode length [cm]—Calculate Plant height at podset and divide by the Total number of nodes on main stem at pod setting.

Total num of pods with seeds on main stem [number]—count all podscontaining seeds on the main stem at pod setting.

Total num of pods with seeds on lateral branches [number]—count all podscontaining seeds on the lateral branches at pod setting.

Total number of pods per plant at pod set [number]—count pods on mainstem and lateral branches at pod setting.

Experimental Results

Twelve different Soybean varieties lines 1-12 were grown andcharacterized for 34 parameters as specified above. The average for eachof the measured parameters was calculated using the JMP software andvalues are summarized in Tables 45-46 below. Subsequent correlationanalysis between the various transcriptome sets and the averageparameters was conducted (Table 47). Follow, results were integrated tothe database.

TABLE 44 Soybean correlated parameters (vectors) Corre- lationCorrelated parameter with ID 100 percent flowering (days) 1 50 percentflowering (days) 2 Base diameter at pod set (mm) 3 DW at pod set (gr) 4Lodging (score 1-5) 5 Maturity (days) 6 Num of lateral branches (number)7 Num of pods with 1 seed on main stem at pod set 8 (number) Num of podswith 2 seed on main stem (number) 9 Num of pods with 3 seed on main stem(number) 10 Num of pods with 4 seed on main stem (number) 11 Plantheight at harvest (cm) 12 Plant height at pod set (cm) 13 Ratio numberof pods per node on main stem (ratio) 14 Ratio number of seeds per mainstem to seeds per 15 lateral branch (ratio) Seed quality (score 1-5) 16Total Number of Seeds on lateral branches 18 Seed size (gr) 18 TotalNumber of Seeds on main stem at pod set 19 Total no of pods with 1 seedon lateral branch (number) 20 Total no of pods with 2 seed on lateralbranch (number) 21 Total no of pods with 3 seed on lateral branch(number) 22 Total no of pods with 4 seed on lateral branch (number) 23Total number of nodes on main stem (number) 24 Total number of nodeswith pods on lateral branches 25 (number) Total number of seeds perplant 26 Total weight of lateral branches at pod set (gr) 27 Totalweight of pods on lateral branches (gr) 28 Total weight of pods on mainstem at pod set (gr) 29 Total weight of pods per plant (gr) 30 Totalweight of seeds per plant (gr/plant ) 31 fresh weight at pod set (gr) 32yield at harvest (bushel/hectare) 33 Average lateral branch seeds perpod (number) 34 Average main stein seeds per pod (number) 35 Main stemaverage internode length(cm/number) 36 Num pods with seeds on lateralbranches-at pod set 37 number) Total number of pods per plant at pod set(number) 38 Total number of pods with seeds on main stem at pod 39 set(number) Corrected Seed size (gr) 40 Table 44.

TABLE 45 Measured parameters in Soybean varieties (lines 1-6) Ecotype/Treatment Line-1 Line-2 Line-3 Line-4 Line-5 Line-6 1 67.33 71.67 67.6767.33 60.00 74.00 2 61.00 65.33 60.67 61.00 54.67 68.33 3 8.33 9.54 9.688.11 8.82 10.12 4 53.67 50.33 38.00 46.17 60.83 55.67 5 1.67 1.83 1.171.67 2.67 2.83 6 24.00 43.67 30.33 30.33 38.33 40.00 7 9.00 8.67 9.119.89 7.67 17.56 8 1.11 4.38 1.44 1.44 4.56 1.67 9 16.89 16.25 13.2216.89 27.00 8.11 10 29.56 1.75 19.78 22.33 11.67 22.78 11 0.00 0.00 0.110.11 0.00 0.44 12 96.67 76.67 67.50 75.83 74.17 76.67 13 86.78 69.5662.44 70.89 69.44 63.89 14 2.87 1.38 2.13 2.26 2.60 1.87 15 0.89 0.900.87 0.89 2.32 0.37 16 2.33 3.50 3.00 2.17 2.83 2.00 17 89.00 219.3393.00 86.00 191.33 71.33 18 150.89 55.89 134.00 160.44 75.44 324.63 19123.56 43.89 87.67 102.67 93.56 88.00 20 1.56 3.00 1.78 1.78 5.67 5.6321 17.00 18.75 26.44 32.33 21.56 33.50 22 38.44 2.00 26.44 31.33 8.8982.00 23 0.00 0.00 0.00 0.00 0.00 1.50 24 16.56 16.78 16.11 18.11 16.7817.11 25 23.00 16.00 23.11 33.00 15.22 45.25 26 274.44 99.78 221.67263.11 169.00 412.50 27 67.78 63.78 64.89 74.89 54.00 167.22 28 26.0014.89 20.11 20.11 21.11 30.25 29 22.11 14.33 16.00 15.00 33.78 9.00 3048.11 29.22 36.11 35.11 54.89 38.88 31 15.09 10.50 17.23 16.51 12.0610.25 32 170.89 198.22 152.56 163.89 224.67 265.00 33 47.57 43.77 50.3756.30 44.00 40.33 34 2.67 1.95 2.43 2.53 2.13 2.68 35 2.60 1.89 2.522.53 2.17 2.59 36 5.24 4.15 3.91 3.92 4.15 3.74 37 57.00 28.56 54.6765.44 36.11 122.63 38 104.56 51.67 89.22 106.22 79.33 155.63 39 47.5623.11 34.56 40.78 43.22 33.00 40 89.00 93.00 86.00 71.33

TABLE 46 Measured parameters in Soybean varieties (lines 7-12) Ecotype/Treatment Line-7 Line-8 Line-9 Line-10 Line-11 Line-12 1 73.00 72.3368.67 73.67 68.00 70.67 2 66.50 65.67 62.33 67.67 61.67 64.33 3 8.468.09 8.26 7.73 8.16 7.89 4 48.00 52.00 44.17 52.67 56.00 47.50 5 2.672.50 1.83 3.50 3.33 1.50 6 41.00 38.33 31.00 39.00 27.33 32.67 7 11.6712.11 8.00 9.11 6.78 10.00 8 4.00 4.33 2.11 1.89 3.44 1.22 9 21.33 17.6720.33 16.11 28.11 16.56 10 11.11 28.22 24.11 36.44 39.67 32.33 11 0.000.56 0.00 3.89 0.00 0.00 12 101.67 98.33 75.83 116.67 76.67 71.67 1389.78 82.11 70.56 101.67 79.56 67.22 14 1.98 2.71 2.78 2.75 3.70 2.84 153.90 0.78 11.18 1.98 1.03 0.83 16 3.50 2.50 2.17 2.33 2.17 2.17 17 88.0075.00 80.67 75.67 76.33 77.33 18 46.88 176.22 143.00 105.44 184.33187.33 19 80.00 126.56 115.11 159.00 178.67 131.33 20 2.88 3.00 1.252.67 1.78 3.00 21 8.50 22.78 21.75 10.67 23.78 25.67 22 9.00 42.11 32.7525.67 45.00 44.33 23 0.00 0.33 0.00 1.11 0.00 0.00 24 18.78 18.89 16.7821.11 19.33 20.78 25 8.25 25.44 21.88 16.33 22.56 24.22 26 136.00 302.78260.50 264.44 363.00 318.67 27 45.44 83.22 64.33 52.00 76.89 67.00 284.13 20.11 17.00 9.22 28.11 27.56 29 9.03 16.00 15.89 14.56 30.44 18.0030 14.25 36.11 32.75 23.78 58.56 40.56 31 7.30 11.38 1568 10.83 12.9815.16 32 160.67 196.33 155.33 178.11 204.44 164.22 33 34.23 44.27 53.6742.47 43.60 52.20 34 2.12 2.58 1.58 2.67 2.62 2.58 35 2.22 2.49 2.472.71 2.51 2.61 36 4.80 4.36 4.20 4.82 4.12 3.83 37 20.38 68.22 55.7540.11 70.56 73.00 38 61.00 119.00 103.25 98.44 141.78 123.11 39 36.4450.78 43.63 58.33 71.22 50.11 40 88.00 75.00 80.67 75.67 76.33 77.33

TABLE 47 Provided are the correlations (R) between the expression levelsyield improving genes and their homologs in various tissues [Expression(Exp) sets] and the phenotypic performance [yield, biomass, and plantarchitecture (Correlation vector (Corr))] under normal conditions acrosssoybean varieties. P = p value. Correlation between the expression levelof selected genes of some embodiments of the invention in varioustissues and the phenotypic performance under normal conditions acrosssoybean varieties Gene Exp. Corr. Gene Exp. Corr. Name R P value set SetID Name R P value set Set ID LYM1216 0.72 4.25E−02 9 7 LYM1216 0.764.35E−03 1 17 LYM1217 0.78 7.37E−03 7 31 LYM1217 0.81 4.15E−03 7 33LYM1217 0.73 7.29E−03 10 23 LYM1218 0.74 1.46E−02 5 10 LYM1218 0.712.18E−02 5 19 LYM1218 0.72 1.82E−02 5 26 LYM1218 0.79 2.08E−02 9 12LYM1218 0.84 6.97E−04 10 23 LYM1219 0.78 7.58E−03 5 7 LYM1219 0.787.26E−03 5 1 LYM1219 0.75 1.23E−02 8 22 LYM1219 0.70 2.31E−02 8 18LYM1219 0.70 2.41E−02 8 2 LYM1219 0.72 1.93E−02 8 26 LYM1219 0.848.33E−03 9 17 LYM1219 0.73 7.16E−03 1 14 LYM1220 0.70 2.38E−02 8 9LYM1220 0.73 3.84E−02 9 22 LYM1220 0.71 4.63E−02 9 18 LYM1220 0.743.41E−02 9 32 LYM1220 0.79 1.92E−02 9 5 LYM1220 0.78 2.15E−02 9 23LYM1220 0.76 2.86E−02 9 27 LYM1220 0.72 4.42E−02 9 7 LYM1220 0.846.63E−04 10 9 LYM1220 0.78 2.69E−03 10 29 LYM1220 0.91 3.43E−05 10 17LYM1221 0.73 1.62E−02 7 5 LYM1221 0.77 9.93E−03 5 14 LYM1221 0.721.92E−02 8 17 LYM1221 0.87 5.35E−03 9 15 LYM1221 0.76 2.95E−02 9 16LYM1222 0.74 1.37E−02 8 11 LYM1222 0.82 3.63E−03 8 23 LYM1222 0.733.83E−02 9 31 LYM1222 0.72 4.48E−02 9 14 LYM1222 0.80 1.82E−02 9 29LYM1222 0.82 9.76E−04 4 9 LYM1222 0.74 6.30E−03 4 30 LYM1222 0.719.31E−03 4 14 LYM1222 0.85 4.82E−04 4 29 LYM1223 0.86 1.36E−03 8 10LYM1223 0.78 7.78E−03 8 19 LYM1223 0.74 3.69E−02 9 31 LYM1223 0.711.01E−02 10 13 LYM1223 0.75 5.28E−03 10 12 LYM1224 0.85 1.79E−03 5 31LYM1224 0.85 1.98E−03 5 33 LYM1224 0.75 3.12E−02 9 29 LYM1224 0.792.37E−03 4 11 LYM1224 0.78 2.88E−03 1 23 LYM1224 0.71 9.14E−03 1 27LYM1225 0.72 8.91E−03 11 5 LYM1224 0.79 2.06E−02 9 17 LYM1225 0.746.42E−03 4 33 LYM1224 0.71 9.14E−03 1 20 LYM1226 0.73 1.60E−02 7 32LYM1225 0.81 1.50E−02 9 15 LYM1226 0.75 3.03E−02 9 13 LYM1225 0.772.68E−02 9 23 LYM1226 0.79 1.99E−02 9 7 LYM1226 0.70 1.09E−02 44 25LYM1227 0.77 8.82E−03 7 21 LYM1227 0.78 7.79E−03 8 10 LYM1227 0.731.68E−02 8 11 LYM1227 0.72 4.31E−02 9 29 LYM1227 0.84 9.05E−03 9 17LYM1227 0.78 3.07E−03 4 17 LYM1227 0.77 3.64E−03 1 17 LYM1227 0.745.73E−03 10 5 LYM1218 0.71 2.16E−02 5 38 LYM1218 0.73 3.88E−02 9 36LYM1219 0.72 8.47E−03 11 36 LYM1219 0.74 1.35E−02 8 35 LYM1219 0.797.15E−03 8 34 LYM1220 0.71 5.02E−02 9 37 LYM1221 0.71 2.05E−02 5 39LYM1223 0.82 3.79E−03 8 35 LYM1223 0.77 9.45E−03 8 34 LYM1223 0.746.02E−03 10 36 LYM1226 0.73 3.97E−02 9 36 LYM1227 0.86 1.33E−03 8 35LYM1227 0.80 5.77E−03 8 34 LYM1227 0.74 9.72E−03 2 35 LYM1216 0.848.59E−03 5 40 LYM1217 0.76 2.98E−02 6 40 LYM1218 0.80 2.92E−02 9 40LYM1219 0.80 1.69E−02 7 40 LYM1219 0.74 1.53E−03 11 40 LYM1219 0.782.32E−02 5 40 LYM1220 0.79 2.03E−02 8 40 LYM1220 0.83 2.20E−02 9 40LYM1222 0.81 4.08E−03 1 40 LYM1223 0.77 2.65E−02 5 40 LYM1224 0.714.83E−02 5 40 LYM1224 0.85 7.12E−03 6 40 LYM1226 0.87 1.00E−02 9 40

Example 10 Production of Tomato Transcriptome and High ThroughputCorrelation Analysis Using 44K Tomato Oligonucleotide Micro-Array

In order to produce a high throughput correlation analysis between NUErelated phenotypes and gene expression, the present inventors utilized aTomato oligonucleotide micro-array, produced by Agilent Technologies[chem. (dot) agilent (dot) com/Scripts/PDS (dot) asp?1Page=50879]. Thearray oligonucleotide represents about 44,000 Tomato genes andtranscripts. In order to define correlations between the levels of RNAexpression with NUE, ABST, yield components or vigor related parametersvarious plant characteristics of 18 different Tomato varieties wereanalyzed. Among them, 10 varieties encompassing the observed variancewere selected for RNA expression analysis. The correlation between theRNA levels and the characterized parameters was analyzed using Pearsoncorrelation test [davidmlane (dot) com/hyperstat/A34739 (dot) html].

Correlation of Tomato Varieties Across Ecotypes Grown Under LowNitrogen, Drought and Regular Growth Conditions

Experimental Procedures:

10 Tomato varieties were grown in 3 repetitive blocks, each containing 6plants per plot were grown at net house. Briefly, the growing protocolwas as follows:

1. Regular growth conditions: Tomato varieties were grown under normalconditions (4-6 Liters/m² of water per day and fertilized with NPK asrecommended in protocols for commercial tomato production).

2. Low Nitrogen fertilization conditions: Tomato varieties were grownunder normal conditions (4-6 Liters/m² per day and fertilized with NPKas recommended in protocols for commercial tomato production) untilflower stage. At this time, Nitrogen fertilization was stopped.

3. Drought stress: Tomato variety was grown under normal conditions (4-6Liters/m² per day) until flower stage. At this time, irrigation wasreduced to 50% compared to normal conditions. Plants were phenotyped ona daily basis following the standard descriptor of tomato (Table 49).Harvest was conducted while 50% of the fruits were red (mature). Plantswere separated to the vegetative part and fruits, of them, 2 nodes wereanalyzed for additional inflorescent parameters such as size, number offlowers, and inflorescent weight. Fresh weight of all vegetativematerial was measured. Fruits were separated to colors (red vs. green)and in accordance with the fruit size (small, medium and large). Next,analyzed data was saved to text files and processed using the JMPstatistical analysis software (SAS institute). Data parameters collectedare summarized in Tables 50-52, herein below.

Analyzed Tomato tissues—Two tissues at different developmental stages[flower and leaf], representing different plant characteristics, weresampled and RNA was extracted as described above. For convenience, eachmicro-array expression information tissue type has received a Set ID assummarized in Table 48 below.

TABLE 48 Tomato transcriptome expression sets Expression Set Set ID Leafat reproductive stage under NUE conditions 1 Flower under normalconditions 2 Leaf at reproductive stage under normal conditions 3 Flowerunder drought conditions 4 Leaf at reproductive stage under droughtconditions 5 Flower under NUE conditions 6 Table 48: Provided are theidentification (ID) digits of each of the tomato expression sets.

Table 49 provides the tomato correlated parameters (Vectors). Theaverage for each of the measured parameter was calculated using the JMPsoftware and values are summarized in Tables 50-52 below. Subsequentcorrelation analysis was conducted (Table 53). Results were integratedto the database.

TABLE 49 Tomato correlated parameters (vectors) Corre- lation Correlatedparameter with ID 100 weight green fruit [g] (Drought) 1 100 weightgreen fruit [g] (Low N) 2 100 weight green fruit [g] (Normal) 3 100weight red fruit [g] (Drought) 4 100 weight red fruit [g] (Low N) 5 100weight red fruit [g] (Normal) 6 Cluster Weight NUE/Normal [g] 7 FWNUE/Normal [g] 8 FW drought/Normal [g] 9 FW/Plant (NUE) [g] 10 FW/Plant(Normal) [g] 11 FW/Plant Drought [g] 12 Fruit Drought/NUE [g] 13 FruitNUE/Normal [g] 14 Fruit Yield Drought/Normal [g] 15 Fruit Yield/Plant(NUE) [g] 16 Fruit Yield/Plant Drought [g] 17 Fruit yield /Plant(Normal) [g] 18 HI [yield/yield + biomass] (Low N) 19 HI [yield/yield +biomass] (Normal) 20 Leaflet Length [cm] (Low N) 21 Leaflet Length [cm](Normal) 22 Leaflet Length [cm]) (Drought) 23 Leaflet Width [cm] (Low N)24 Leaflet Width [cm] (Normal) 25 Leaflet Width [cm] (Drought) 26 NUE[yield/SPAD] (Low N) 27 NUE [yield/SPAD] (Normal) 28 NUE2 [totalbiomass/SPAD] (Low N) 29 NUE2 [total biomass/SPAD] (Normal) 30 NUpE[biomass/SPAD] (Low N) 31 NUpE [biomass/SPAD] (Normal) 32 No flowers(NUE) 33 No flowers (Normal) 34 Num of Flower Drought/NUE 35 Num ofFlower Drought/Normal 36 Num of flowers (Drought) 37 Num. FlowersNUE/Normal 38 RWC (Normal) 39 RWC Drought 40 RWC Drought/Normal 41 RWCNUE 42 RWC NUE/Normal 43 SAPD 100% RWC NUE/Normal 44 SLA [leafarea/plant biomass] (Low N) 45 SLA [leaf area/plant biomass] (Normal) 46SPAD (Normal) 47 SPAD 100% RWC (NUE) 48 SPAD 100% RWC (Normal) 49 SPADNUE 50 SPAD NUE/Normal 51 Total Leaf Area [cm²] (Low N) 52 Total LeafArea [cm²] (Normal) 53 Total Leaf Area [cm²]) (Drought) 54 Weight Flowerclusters [g] (Normal) 55 Weight clusters (flowers) [g] (NUE) 56 Weightflower clusters [g] (Drought) 57 Yield/SLA (Low N) 58 Yield/SLA (Normal)59 Yield/total leaf area (Low N) 60 Yield/total leaf area (Normal) 61average red fruit weight [g] (NUE) 62 average red fruit weight [g](Normal) 63 average red fruit weight [g] (Drought) 64 flower clusterweight Drought/NUE [g] 65 flower cluster weight Drought/Normal [g] 66red fruit weight Drought/Normal [g] 67 Table 49. Provided are the tomatocorrelated parameters. “gr.” = grams; “FW” = fresh weight; “NUE” =nitrogen use efficiency; “RWC” = relative water content; “NUpE” =nitrogen uptake efficiency; “SPAD” = chlorophyll levels; “HI” = harvestindex (vegetative weight divided on yield); “SLA” = specific leaf area(leaf area divided by leaf dry weight), Treatment in the parenthesis.

Fruit Weight (grams)—At the end of the experiment [when 50% of thefruits were ripe (red)] all fruits from plots within blocks A-C werecollected. The total fruits were counted and weighted. The averagefruits weight was calculated by dividing the total fruit weight by thenumber of fruits.

Plant vegetative Weight (grams)—At the end of the experiment [when 50%of the fruit were ripe (red)] all plants from plots within blocks A-Cwere collected. Fresh weight was measured(.grams).

Inflorescence Weight (grams)—At the end of the experiment [when 50% ofthe fruits were ripe (red)] two Inflorescence from plots within blocksA-C were collected.

The Inflorescence weight (gr.) and number of flowers per inflorescencewere counted.

SPAD—Chlorophyll content was determined using a Minolta SPAD502chlorophyll meter and measurement was performed at time of flowering.SPAD meter readings were done on young fully developed leaf. Threemeasurements per leaf were taken per plot.

Water use efficiency (WUE)—can be determined as the biomass produced perunit transpiration. To analyze WUE, leaf relative water content wasmeasured in control and transgenic plants. Fresh weight (FW) wasimmediately recorded; then leaves were soaked for 8 hours in distilledwater at room temperature in the dark, and the turgid weight (TW) wasrecorded. Total dry weight (DW) was recorded after drying the leaves at60° C. to a constant weight. Relative water content (RWC) was calculatedaccording to the following Formula I[(FW−DWITW−DW)×100] as describedabove.

Plants that maintain high relative water content (RWC) compared tocontrol lines were considered more tolerant to drought than thoseexhibiting reduced relative water content

Experimental Results

TABLE 50 Measured parameters in Tomato accessions (lines 1-6)Ecotype/Treatment Line-1 Line-2 Line-3 Line-4 Line-5 Line-6 2 0.87 3.660.57 0.37 3.40 0.68 3 0.56 3.05 0.24 2.58 5 1.06 6.87 0.65 0.53 7.170.44 6 0.82 2.46 0.50 2.76 7 0.46 1.07 0.44 0.01 1.08 0.02 8 2.65 0.380.74 3.01 0.83 1.54 9 1.72 0.34 0.61 2.63 1.18 1.36 10 4.04 1.21 2.252.54 1.85 3.06 11 1.53 3.17 3.02 0.84 2.24 1.98 17 2.62 1.09 1.85 2.222.63 2.71 13 1.15 0.73 1.32 0.76 1.51 0.71 14 0.49 1.93 0.97 3.80 2.780.78 15 0.57 1.41 1.27 2.88 4.20 0.55 16 0.41 0.66 0.48 0.46 1.35 0.3517 0.47 0.48 0.63 0.35 2.04 0.25 18 0.83 0.34 0.49 0.12 0.49 0.45 190.09 0.35 0.18 0.15 0.42 0.10 20 0.35 0.10 0.14 0.12 0.18 0.19 21 6.405.92 3.69 5.43 6.95 3.73 22 6.34 7.99 5.59 7.70 24 3.47 1.97 1.79 2.553.52 1.73 25 3.69 4.77 3.43 4.56 27 0.014 0.017 0.014 0.020 0.039 0.01128 0.017 0.009 0.009 0.003 0.010 0.010 29 0.156 0.048 0.082 0.128 0.0930.105 30 0.047 0.095 0.063 0.021 0.057 0.056 31 0.142 0.031 0.068 0.1080.054 0.094 32 0.031 0.085 0.054 0.018 0.046 0.046 33 19.00 5.33 9.0013.00 10.67 16.67 34 5.67 19.33 6.33 7.67 9.67 8.33 35 0.88 1.22 1.741.56 1.09 1.52 36 2.94 0.34 2.47 2.65 1.21 3.04 37 16.67 6.50 15.6720.33 11.67 25.33 38 3.35 0.28 1.42 1.70 1.10 2.00 39 72.83 76.47 64.2967.07 54.79 77.61 40 72.12 74.51 65.33 72.22 66.13 68.33 41 0.99 0.971.02 1.08 1.21 0.88 42 74.07 99.08 69.49 63.24 77.36 77.91 43 1.02 1.301.08 0.94 1.41 1.00 44 0.79 1.37 0.92 0.75 1.31 0.97 45 140.04 317.12131.29 148.82 257.51 64.34 46 140.99 689.67 130.22 299.12 47 49.70 37.2055.80 46.40 48.20 43.40 48 28.47 39.04 33.01 23.42 34.53 32.51 49 36.1728.45 35.89 31.09 26.38 33.68 50 38.40 39.40 47.50 37.00 44.60 41.70 510.77 1.06 0.85 0.80 0.93 0.96 52 565.93 384.77 294.83 378.00 476.39197.08 53 426.10 582.38 291.40 593.58 55 1.17 0.34 0.69 56.35 0.44 11.3156 0.53 0.37 0.31 0.35 0.47 0.25 57 0.37 0.41 0.33 0.29 0.55 0.31 580.003 0.002 0.004 0.003 0.005 0.006 59 0.004 0.000 0.004 0.002 60 0.0010.002 0.002 0.001 0.003 0.002 61 0.001 0.000 0.002 0.001 62 0.02 0.190.01 0.01 0.10 0.00 63 0.05 0.01 0.01 0.29 0.01 0.05 64 0.01 0.19 0.210.00 0.10 0.00 65 0.69 1.11 1.06 0.82 1.16 1.25 66 0.32 1.19 0.47 0.011.25 0.03 67 0.19 24.37 25.38 0.02 20.26 0.04

TABLE 51 Measured paramaers in Tomato accessions (lines 7-12)Ecotype/Treatment Line-7 Line-8 Line-9 Line-10 Line-11 Line-12 1 0.80 20.45 0.47 0.54 0.39 0.97 0.91 3 6.32 5.75 0.38 0.30 1.95 2.53 4 0.89 50.55 0.75 0.58 1.27 1.34 6 5.32 5.24 0.61 0.66 2.70 0.70 7 0.37 0.810.55 0.36 0.95 0.80 8 3.70 1.22 0.58 0.55 1.06 0.49 9 4.02 1.01 0.610.64 0.95 0.51 10 3.13 2.54 1.84 1.52 1.91 1.86 11 0.85 2.09 3.21 2.751.81 3.77 12 3.41 2.11 1.95 1.76 1.72 1.92 13 5.06 0.89 0.67 2.17 0.381.27 14 0.02 1.16 2.07 1.51 2.41 2.06 15 0.09 1.03 1.39 3.28 0.91 2.6216 0.01 0.51 0.44 0.47 1.59 0.39 17 0.05 0.45 0.29 1.02 0.60 0..49 180.53 0.44 0.21 0.31 0.66 0.19 19 0.00 0.17 0.19 0.24 0.45 0.17 20 0.380.17 0.06 0.10 0.77 0.05 21 4.39 6.72 6.66 4.39 3.90 5.29 22 7.85 6.226.16 5.65 4.39 4.44 23 5.15 24 1.87 3.54 3.28 2.52 2.61 2.61 75 4.443.15 3.37 3.13 2.40 2.02 26 2.55 27 0.000 0.015 0.014 0.013 0.064 0.01028 0.012 0.008 0.004 0.006 0.017 0.004 29 0.114 0.091 0.076 0.056 0.1410.055 30 0.032 0.047 0.058 0.060 0.062 0.083 31 0.113 0.075 0.061 0.0430.077 0.046 32 0.020 0.039 0.055 0.054 0.045 0.079 33 6.00 16.00 15.006.00 17.00 13.00 34 5.00 8.33 10.00 7.00 9.00 8.00 35 4.96 1.08 0.984.94 0.88 0.79 36 5.95 2.08 1.47 4.24 1.67 1.29 37 29.73 17.33 14.6729.67 15.00 10.33 38 1.20 1.92 1.50 0.86 1.89 1.63 39 58.18 66.51 64.7175.25 66.23 63.21 40 78.13 18.46 73.21 62.50 67.21 75.76 41 1.34 0.281.13 0.83 1.01 1.20 42 80.49 67.40 67.16 66.07 69.57 69.30 43 1.38 1.011.04 0.88 1.05 1.10 44 1.11 0.95 0.79 0.97 0.94 1.36 45 144.60 246.05405.55 299.32 86.19 182.32 46 1117.74 111.77 106.29 123.14 104.99 111.8847 42.90 53.30 58.50 51.10 40.00 47.60 48 27.66 33.68 30.04 35.50 24.8140.77 49 24.98 35.47 37.87 38.43 26.49 30.07 50 34.40 50.00 44.70 53.7035.70 58.80 51 0.80 0.94 0.76 1.05 0.89 1.24 52 453.2.4 625.51 748.01453.96 164.85 338.30 53 947.59 233.35 340.73 339.11 190.14 421.79 54337.63 55 0.79 0.58 0.73 0.83 0.86 0.50 56 0.29 0.47 0.40 0.30 0.82 0.4057 0.45 0.56 0.30 0.31 0.31 0.31 58 0.000 0.002 0.001 0.002 0.018 0.00259 0.000 0.004 0.002 0.003 0.006 0.002 60 0.000 0.001 0.001 0.001 0.0100.001 61 0.001 0.002 0.001 0.001 0.003 0.000 62 0.01 0.01 0.01 0.01 0.020.01 63 0.23 0.29 0.01 0.01 0.06 0.01 64 0.03 0.01 0.01 0.00 0.01 0.0165 1.52 1.19 0.76 1.04 0.38 0.78 66 0.56 0.96 0.42 0.38 0.36 0.62 670.15 0.02 0.86 0.74 0.09 1.72

TABLE 52 Provided are the values of each of the parameters (as describedabove) measured in tomato accessions (Seed ID) under all growthconditions. Growth conditions are specified in the experimentalprocedure section. Measured parameters in Tomato accessions (lines13-18) Ecotype/Treatment Line-13 Line-14 Line-15 Line-16 Line-17 Line-181 0.28 0.38 0.63 2.86 1.16 4.40 2 0.36 0.35 0.57 4.38 2.02 8.13 3 1.422.03 1.39 2.27 0.45 0.42 4 0.35 0.63 2.27 7.40 2.94 11.60 5 0.52 0.570.94 6.17 3.67 11.33 6 2.64 4.67 2.17 0.49 0.34 0.75 7 0.34 0.61 0.940.68 0.40 1.44 8 1.31 1.36 0.51 0.71 0.31 0.47 9 1.17 1.94 0.35 1.060.21 0.48 10 2.47 2.62 1.08 1.17 0.92 1.09 11 1.89 1.93 2.14 1.65 3.012.29 12 2.21 3.73 0.75 1.76 0.63 1.11 13 0.84 1.51 0.98 1.34 0.38 0.8414 0.38 1.64 0.41 1.21 4.59 1.70 15 0.32 2.48 0.41 1.62 1.76 1.42 160.32 0.45 0.14 0.40 1.44 0.50 17 0.27 0.68 0.14 0.53 0.55 0.41 18 0.850.27 0.35 0.33 0.31 0.29 19 0.12 0.15 0.12 0.25 0.61 0.31 20 0.31 0.120.14 0.17 0.09 0.11 21 6.32 5.11 4.72 6.83 7.10 8.21 22 6.77 7.42 6.715.87 4.16 10.29 23 3.38 7.14 5.48 8.62 6.35 6.77 24 3.58 2.56 2.48 3.433.30 3.69 25 3.80 3.74 2.98 3.22 2.09 5.91 26 2.04 4.17 3.09 4.69 3.872.91 27 0.007 0.017 0.004 0.013 0.037 0.013 28 0.015 0.006 0.008 0.0060.008 0.005 29 0.059 0.118 0.035 0.051 0.061 0.042 30 0.047 0.046 0.0570.036 0.080 0.044 31 0.052 0.101 0.031 0.038 0.024 0.029 32 0.033 0.0400.049 0.030 0.072 0.039 33 8.67 9.33 12.67 6.67 9.33 8.00 34 5.33 8.007.67 9.00 10.67 9.00 35 2.12 1.29 1.61 1.90 1.36 1.42 36 3.44 1.50 2.651.41 1.19 1.26 37 18.33 12.00 20.33 12.67 12.67 11.33 38 1.63 1.17 1.650.74 0.88 0.89 39 56.77 35.96 77.62 100.00 63.16 75.13 40 62.82 70.6955.75 75.22 63.68 62.31 41 1.11 1.97 0.72 0.75 1.01 0.83 42 100.00 57.6690.79 68.00 59.65 72.17 43 1.76 1.60 1.17 0.68 0.94 0.96 44 1.44 1.501.05 0.56 1.48 0.84 45 160.18 90.10 160.99 379.03 531.08 650.68 46307.95 419.37 365.81 212.93 84.94 469.87 47 57.90 48.30 43.60 54.5041.60 59.10 48 47.47 26.06 35.38 30.60 38.97 37.46 49 32.89 17.35 33.8254.47 26.25 44.43 50 47.50 45.20 39.00 45.00 65.30 51.90 51 0.82 0.940.89 0.83 1.57 0.88 52 396.00 236.15 174.58 441.78 489.18 707.80 53581.33 807.51 784.06 351.80 255.78 1078.10 54 130.78 557.93 176.67791.86 517.05 832.27 55 1.02 0.70 0.38 0.66 0.70 0.33 56 0.35 0.43 0.350.45 0.28 0.47 57 8.36 0.29 0.34 0.44 0.27 0.43 58 0.002 0.005 0.0010.001 0.003 0.001 59 0.003 0.001 0.001 0.002 0.004 0.001 60 0.001 0.0020.001 0.001 0.003 0.001 61 0.001 0.000 0.000 0.001 0.001 0.000 62 0.010.05 0.36 0.04 0.63 63 0.03 0.26 0.03 0.00 0.00 0.01 64 0.00 0.01 0.300.14 0.04 0.09 65 24.12 0.67 0.97 0.99 0.95 0.91 66 8.20 0.41 0.91 0.670.38 1.31 67 0.17 0.02 10.50 27.89 11.79 9.98

TABLE 53 Provided are the correlations (R) between the expression levelsyield improving genes and their homologs in various tissues [Expression(Exp) sets] and the phenotypic performance [yield, biomass, growth rateand/or vigor components (Correlation vector (Cor))] under normalconditions across tomato ecotypes, P = p value Correlation between theexpression level of selected genes of some embodiments of the inventionin various tissues and the phenotypic performance under normal andstress conditions across tomato ecotypes Gene Exp. Corr. Gene Exp. Corr.Name R P value set Set ID Name R P value set Set ID LYM1228 0.787.60E−03 1 7 LYM1228 0.89 5.31E−04 1 2 LYM1228 0.84 2.26E−03 1 5 LYM12280.74 1.41E−02 5 67 LYM1228 0.82 3.57E−03 5 64 LYM1229 0.92 5.03E−04 3 20LYM1229 0.82 6.77E−03 3 28 LYM1229 0.73 1.55E−02 6 10 LYM1229 0.731.64E−02 6 8 LYM1229 0.73 1.73E−02 6 38 LYM1230 0.83 3.24E−03 5 35LYM1231 0.71 3.20E−02 3 32 LYM1231 0.77 2.52E−02 2 61 LYM1232 0.761.69E−02 3 32 LYM1232 0.75 1.98E−02 3 30 LYM1232 0.78 8.00E−03 6 33LYM1233 0.70 5.12E−02 2 3 LYM1233 0.71 2.15E−02 2 47 LYM1233 0.721.99E−02 6 29 LYM1233 0.84 2.26E−03 5 67 LYM1233 0.88 9.01E−04 5 64LYM1240 0.75 2.03E−02 1 62 LYM1240 0.76 3.02E−02 2 22 LYM1240 0.705.10E−02 2 46 LYM1240 0.79 1.91E−02 2 53 LYM1240 0.77 2.51E−02 2 25

Example 11 Production of Maize Transcriptome and High ThroughputCorrelation Analysis with Yield, NUE, and ABST Related ParametersMeasured in Semi-Hydroponics Conditions Using 60K Maize OligonucleotideMicro-Arrays

Maize vigor related parameters under low nitrogen, 100 mM NaCl, lowtemperature (10±2° C.) and normal growth conditions—twelve Maize hybridswere grown in 5repetitive plots, each containing 7plants, at a net houseunder semi-hydroponics conditions. Briefly, the growing protocol was asfollows: Maize seeds were sown in trays filled with a mix of vermiculiteand peat in a 1:1 ratio. Following germination, the trays weretransferred to the high salinity solution (100 mM NaCl in addition tothe Full Hoagland solution), low temperature (10±2° C. in the presenceof Full Hoagland solution), low nitrogen solution (the amount of totalnitrogen was reduced in 90% from the full Hoagland solution (i.e., to afinal concentration of 10% from full Hoagland solution, final amount of1.6 mM N) or at Normal growth solution (Full Hoagland containing 16 mM Nsolution, at 28 2° C.). Plants were grown at 28 i 2° C.

Full Hoagland solution consists of: KNO₃—0.808 grams/liter, MgSO₄—0.12grams/liter, KH₂PO₄—0.136 grams/liter and 0.01% (volume/volume) of‘Super coratin’ micro elements (Iron-EDDHA[ethylenediamine-N,N′-bis(2-hydroxyphenylacetic acid)]—40.5 grams/liter;Mn—20.2 grams/liter; Zn 10.1 grams/liter; Co 1.5 grams/liter; and Mo 1.1grams/liter), solution's pH should be 6.5-6.8].

Analyzed maize tissues—Twelve selected maize hybrids were sampled pereach treatment. Two tissues [leaves and root tip] growing at 100 mMNaCl, low temperature (10 i 2° C.), low Nitrogen (1.6 mM N) or underNormal conditions were sampled at the vegetative stage (V4-5) and RNAwas extracted as described above. Each micro-array expressioninformation tissue type has received a Set ID as summarized in Tables54-57 below.

TABLE 54 Maize transcriptome expression sets under semi hydroponicsconditions Expression set Set ID leaf at vegetative stage (V4-V5) underNormal conditions 1 root tip at vegetative stage (V4-V5) under Normalconditions 2 Table 54: Provided are the Maize transcriptome expressi.onsets at normal conditions.

TABLE 55 Maize transcriptome expression sets under semi hydroponicsconditions Expression set Set ID leaf at vegetative stage (V4-V5) undercold conditions 1 root tip at vegetative stage (V4-V5) under coldconditions 2 Table 55: Provided are the Maize transcriptotne expressionsets at cold conditions.

TABLE 56 Maize transcriptome expression sets under semi hydroponicsconditions Expression set Set ID leaf at vegetative stage V4-V5) underlow N conditions (1.6 mM 1 N) root tip at vegetative stage (V4-V5) underlow N conditions (1.6 2 mM N) Table 56: Provided are the Maizetranscriptome expression sets at low nitrogen conditions 1.6 MmNitrogen.

TABLE 57 Maize transcriptome expression sets under semi hydroponicsconditions Expression set Set ID leaf at vegetative stage (V4-V5) undersalinity conditions (NaCl 1 100 mM) root tip at vegetative stage (V4-V5)under salinity conditions 2 (NaCl 100 mM) Table 57: Provided are theMaize transcriptome expression sets at 100 mM NaCl.

Experimental Results

12 different Maize hybrids were grown and characterized at thevegetative stage (V4-5) for the following parameters: “Leaves DW”=leavesdry weight per plant (average of five plants); “Plant Height growth”=wascalculated as regression coefficient of plant height [cm] along timecourse (average of five plants); “Root DW”—root dry weight per plant,all vegetative tissue above ground (average of four plants); “ShootDW”—shoot dry weight per plant, all vegetative tissue above ground(average of four plants) after drying at 70° C. in oven for 48 hours;“Shoot FW”—shoot fresh weight per plant, all vegetative tissue aboveground (average of four plants); “SPAD”—Chlorophyll content wasdetermined using a Minolta SPAD 502 chlorophyll meter and measurementwas performed 30 days post sowing. SPAD meter readings were done onyoung fully developed leaf. Three measurements per leaf were taken perplot. The average for each of the measured parameter was calculated andvalues are summarized in Tables 59-66 below. Subsequent correlationanalysis was performed (Table 67-70). Results were then integrated tothe database.

TABLE 58 Maize correlated parameters (vectors) Correlated parameter withCorrelation ID Leaves DW (g) 1 Plant height growth (cm/day) 2 Root DW(g) 3 Root length (cm) 4 SPAD 5 Shoot DW (g) 6 Shoot FW (g) 7 Table 58:Provided are the Maize correlated parameters.

TABLE 59 Provided are the values of each of the parameters (as describedabove, measured in Maize accessions (Seed ID) under low nitrogenconditions. Growth conditions are specified in the experimentalprocedure section. Maize accessions, measured parameters under lownitrogen growth conditions Ecotype/ Treatment Line-1 Line-2 Line-3Line-4 Line-5 Line-6 1 0.57 0.45 0.46 0.48 0.36 0.51 2 0.75 0.81 0.880.69 0.83 0.84 3 0.38 0.35 0.25 0.36 0.31 0.30 4 44.50 45.63 44.25 43.5940.67 42.03 5 21.43 21.24 22.23 24.56 27.75 26.47 6 2.56 1.96 2.01 1.941.94 2.52 7 23.27 20.58 19.26 20.07 17.98 22.06

TABLE 60 Maize accessions, measured parameters under low nitrogen growthconditions Ecotype/ Treatment Line-7 Line-8 Line-9 Line-10 Line-11Line-12 1 0.53 0.58 0.55 0.51 0.56 0.39 2 0.78 0.92 0.89 0.85 0.80 0.643 0.29 0.31 0.29 0.32 0.43 0.17 4 42.65 45.06 45.31 42.17 41.03 37.65 522.08 25.09 23.73 25.68 25.02 19.51 6 2.03 2.37 2.09 2.17 2.62 1.53 721.28 22.13 20.29 19.94 22.50 15.93 Table 60: Provided are the values ofeach of the parameters (as described above) measured in Maize accessions(Seed ID) under low nitrogen conditions. Growth conditions are specifiedin the experimental procedure section.

TABLE 61 Maize accessions, measured parameters under 100 mM NaCl growthconditions Ecotype/Treatment Line-1 Line-2 Line-3 Line-4 Line-5 Line-6 10.41 0.50 0.43 0.48 0.43 0.56 2 0.457 0.398 0.454 0.316 0.322 0.311 30.047 0.050 0.030 0.071 0.046 0.031 4 10.88 11.28 11.82 10.08 8.46 10.565 36.55 39.92 37.82 41.33 40.82 44.40 6 2.43 2.19 2.25 2.26 1.54 1.94 719.58 20.78 18.45 19.35 15.65 16.09 Table 61: Provided are the values ofeach of the parameters (as described above) measured in Maize accessions(Seed ID) under 100 mM NaCl growth conditions. Growth conditions arespecified in the experimental procedure section.

TABLE 62 Maize accessions, measured parameters under 100 mM NaCl growthconditions Ecotype/Treatment Line-7 Line-8 Line-9 Line-10 Line-11Line-12 1 0.33 0.51 0.47 0.98 0.48 0.15 2 0.290 0.359 0.370 0.355 0.3050.272 3 0.095 0.063 0.016 0.035 0.049 0.015 4 10.14 11.83 10.55 11.1810.09 8.90 5 37.92 43.22 39.83 38.20 38.14 37.84 6 1.78 1.90 1.89 2.201.86 0.97 7 12.46 16.92 16.75 17.64 15.90 9.40 Table 62: Provided arethe values of each of the parameters (as described above) measured inMaize accessions (Seed ID) under 100 mM NaCl growth conditions. Growthconditions are specified in the experimental procedure section.

TABLE 63 Maize accessions, measured parameters under cold growthconditions Ecotype/Treatment Line-1 Line-2 Line-3 Line-4 Line-5 Line-6 11.19 1.17 1.02 1.18 1.04 1.23 2 2.15 1.93 2.12 1.80 2.32 2.15 3 0.0470.068 0.100 0.081 0.066 0.067 4 28.88 29.11 27.08 32.38 32.68 32.89 55.74 4.86 3.98 4.22 4.63 4.93 6 73.79 55.46 53.26 54.92 58.95 62.36Table 63: Provided are the values of each of the parameters (asdescribed above) measured in Maize accessions (Seed ID) under coldgrowth conditions. Growth conditions are specified in the experimentalprocedure section.

TABLE 64 Maize accessions, measured parameters under cold growthconditions Ecotype/Treatment Line-7 Line-8 Line-9 Line-10 Line-11Line-12 1 1.13 0.98 0.88 1.28 1.10 0.60 2 2.49 2.01 1.95 2.03 1.85 1.213 0.137 0.067 0.073 0.020 0.052 0.057 4 31.58 33.01 28.65 31.43 30.6430.71 5 4.82 4.03 3.57 3.99 4.64 1.89 6 63.65 54.90 48.25 52.83 55.0829.61 Table 64: Provided are the values of each of the parameters (asdescribed above) measured in Maize accessions (Seed ID) under coldgrowth conditions. Growth conditions are specified in the experimentalprocedure section.

TABLE 65 Maize accessions, measured parameters under regular growthconditions Ecotype/Treatment Line-1 Line-2 Line-3 Line-4 Line-5 Line-6 11.161 1.099 0.924 1.013 0.935 0.907 2 1.994 1.919 1.927 1.934 2.1521.948 3 0.140 0.106 0.227 0.155 0.077 0.049 4 20.15 15.89 18.59 18.7216.38 14.93 5 34.50 35.77 34.70 34.42 35.26 37.52 6 5.27 4.67 3.88 5.084.10 4.46 7 79.00 62.85 59.73 63.92 60.06 64.67 Table 65: Provided arethe values of each of the parameters (as described above) measured inMaize accessions (Seed ID) under regular (non-stress) growth conditions.Growth conditions are specified in the experimental procedure section.

TABLE 66 Maize accessions, measured parameters under regular growthconditions Ecotype/Treatment Line-7 Line-8 Line-9 Line-10 Line-11Line-12 1 1.105 1.006 1.011 1.02 1.23 0.44 2 2.234 1.937 1.965 2.05 1.741.26 3 0.175 0.101 0.069 0.10 0.14 0.03 4 17.48 15.74 15.71 17.58 16.1317.43 5 36.50 36.07 33.74 34.34 35.74 29.04 6 4.68 4.59 4.08 4.61 5.422.02 7 68.10 65.81 58.31 61.87 70.04 35.96 Table 66: Provided are thevalues of each of the parameters (as described above) measured in Maizeaccessions (Seed ID) under regular (non-stress) growth conditions.Growth conditions are specified in the experimental procedure section.

TABLE 67 Correlation between the expression level of selected genes ofsome embodiments of the invention in various tissues and the phenotypicperformance under normal conditions across Maize accessions Gene Exp.Corr. Name R P value set Set ID LYM1130 0.70 3.52E−02 2 6 LYM1142 0.741.51E−02 1 4 LYM1151 0.75 1.88E−02 2 7 LYM1151 0.75 1.94E−02 2 3 LYM11560.70 2.38E−02 1 4 LYM1167 0.79 1.06E−02 2 6 LYM1171 0.73 1.65E−02 1 3LYM1176 0.79 6.96E−03 1 7 LYM1130 0.75 1.22E−02 1 2 LYM1151 0.835.31E−03 2 1 LYM1151 0.84 5.03E−03 2 6 LYM1163 0.71 2.10E−02 1 7 LYM11670.74 1.50E−02 1 4 LYM1171 0.71 2.18E−02 1 4 LYM1176 0.76 1.06E−02 1 2Table 67. Provided are the correlations (R) between the expressionlevels of yield improving genes and their homologues in tissues [Leavesor roots; Expression sets (Exp)] and the phenotypic performance invarious biomass, growth rate and/or vigor components [Correlation vector(corr.)] under normal conditions across Maize accessions. P = p value.

TABLE 68 Correlation between the expression level of selected genes ofsome embodiments of the invention in various tissues and the phenotypicperformance under low nitrogen conditions across Maize accessions GeneExp. Corr. Name R P value set Set ID LYM1139 0.70 3.48E−02 2 5 LYM11430.88 1.97E−03 2 7 LYM1143 0.73 2.55E−02 2 6 LYM1161 0.70 2.40E−02 1 4LYM1164 0.87 1.16E−03 1 4 LYM1181 0.72 3.03E−02 2 7 LYM1182 0.771.52E−02 2 5 LYM1141 0.74 2.22E−02 2 4 LYM1143 0.85 3.53E−03 2 1 LYM11540.76 1.06E−02 1 3 LYM1162 0.76 1.07E−02 1 5 LYM1170 0.74 1.46E−02 1 5LYM1181 0.78 1.24E−02 2 1 LYM1184 0.73 2.47E−02 2 3 Table 68. Providedare the correlations (R) between the expression levels of yieldimproving genes and their homologues in tissues [Leaves or roots;Expression sets (Exp)] and the phenotypic performance in variousbiomass, growth rate and/or vigor components [Correlation vector(corr.)] under low nitrogen conditions across Maize accessions. P = pvalue.

TABLE 69 Correlation between the expression level of selected genes ofsome embodiments of the invention in various tissues and the phenotypicperformance under cold conditions across Maize accessions Gene Exp.Corr. Name R P value set Set ID LYM1129 0.71 4.91E−02 1 3 LYM1130 0.883.86E−03 1 2 LYM1130 0.85 3.45E−03 2 1 LYM1137 0.81 1.54E−02 1 5 LYM11390.80 1.82E−02 1 2 LYM1142 0.74 3.52E−02 1 2 LYM1149 0.70 5.13E−02 1 7LYM1154 0.72 2.83E−02 2 2 LYM1158 0.87 4.48E−03 1 1 LYM1159 0.733.89E−02 1 7 LYM1159 0.75 3.19E−02 1 3 LYM1164 0.71 5.07E−02 1 3 LYM11690.70 3.47E−02 2 5 LYM1176 0.74 3.75E−02 1 7 LYM1177 0.74 3.65E−02 1 2LYM1185 0.70 3.56E−02 2 6 LYM1187 0.75 3.11E−02 1 3 LYM1130 0.875.50E−03 1 7 LYM1130 0.83 1.03E−02 1 6 LYM1130 0.71 3.16E−02 2 6 LYM11390.75 3.30E−02 1 7 LYM1139 0.71 4.70E−02 1 5 LYM1146 0.73 3.92E−02 1 7LYM1149 0.80 1.81E−02 1 2 LYM1158 0.81 1.54E−02 1 7 LYM1158 0.762.96E−02 1 6 LYM1159 0.87 4.89E−03 1 2 LYM1162 0.76 2.95E−02 1 3 LYM11670.71 5.06E−02 1 7 LYM1170 0.85 3.57E−03 2 3 LYM1176 0.81 1.49E−02 1 2LYM1185 0.73 4.05E−02 1 5 LYM1186 0.74 3.76E−02 1 1 Table 69. Providedare the correlations (R) between the expression levels of yieldimproving genes and their homologues in tissues [Leaves or roots;Expression sets (Exp)] and the phenotypic performance in variousbiomass, growth rate and/or vigor components [Correlation vector(corr.)] under cold conditions (10 ± 2° C.) across Maize accessions, P =p value.

TABLE 70 Correlation between the expression level of selected genes ofsome embodiments of the invention in various tissues and the phenotypicperformance under salinity conditions across Maize accessions Gene Exp.Corr. Name R P value set Set lD LYM1129 071 3.17E−02 2 7 LYM1135 0832.75E−03 1 2 LYM1140 0.78 1.36E−02 2 3 LYM1142 0.73 2.49E−02 2 2 LYM11420.78 7.64E−03 1 2 LYM1152 0.76 1.70E−02 2 1 LYM1154 0.87 2.26E−03 2 5LYM1154 0.78 7.90E−03 1 5 LYM1162 0.80 5.37E−03 1 2 LYM1163 0.713.26E−02 2 4 LYM1169 0.82 6.71E−03 2 1 LYM1169 0.85 1.82E−03 1 2 LYM11770.72 1.83E−02 1 2 LYM1186 0.75 1.89E−02 2 3 LYM1134 0.78 1.28E−02 2 2LYM1139 0.77 1.49E−02 2 7 LYM1141 0.74 2.13E−02 2 7 LYM1142 0.796.50E−03 1 7 LYM1142 0.72 1.89E−02 1 6 LYM1146 0.77 1.52E−02 2 5 LYM11540.83 5.84E−03 2 1 LYM1158 0.86 3.11E−03 2 7 LYM1162 0.86 1.37E−03 1 5LYM1169 0.87 2.11E−03 2 7 LYM1169 0.92 5.16E−04 2 6 LYM1170 0.809.90E−03 2 5 LYM1184 0.76 1.07E−02 1 3 LYM1187 0.86 1.56E−03 1 3 Table70. Provided are the correlations (R) between the expression levels ofyield improving genes and their homologues in tissues [Leaves or roots;Expression sets (Exp)] and the phenotypic performance in variousbiomass, growth rate and/or vigor components [Correlation vector(corr.)] under salinity conditions (100 mM NaCl) across Maizeaccessions. P = p value.

Example 12 Production of Sorghum Transcriptome and High ThroughputCorrelation Analysis Using 60K Sorghum Oligonucleotide Micro-Array

In order to produce a high throughput correlation analysis between plantphenotype and gene expression level, the present inventors utilized asorghum oligonucleotide micro-array, produced by Agilent Technologies[chem. (dot) agilent (dot) com/Scripts/PDS (dot) asp?1Page=50879]. Thearray oligonucleotide represents about 60,000 sorghum genes andtranscripts. In order to define correlations between the levels of RNAexpression with vigor related parameters, various plant characteristicsof 10 different sorghum hybrids were analyzed. The correlation betweenthe RNA levels and the characterized parameters was analyzed usingPearson correlation test [davidmlane (dot) com/hyperstat/A34739 (dot)html].

Experimental Procedures

Correlation of Sorghum varieties across ecotypes grown in growthchambers under temperature of 30° C. or 14° C. at low light (100 μE) andhigh light (250 μE) conditions.

Analyzed Sorghum tissues—All 10 selected Sorghum hybrids were sample pereach condition. Leaf tissue growing under 30° C. and low light (100 μEm⁻² sec⁻¹), 14° C. and low light (100 μE m⁻² sec⁻¹), 30° C. and highlight (250 μE m⁻² sec⁻¹), 14° C. and high light (250 μE m⁻² sec⁻¹) weresampled at vegetative stage of four-five leaves and RNA was extracted asdescribed above. Each micro-array expression information tissue type hasreceived a Set ID as summarized in Table 71 below.

TABLE 71 Sorghum transcriptome expression sets in field experimentsSorghum/leaf; 14 Celsius degree; high light; light on 1 Sorghum/leaf; 14Celsius degree; low light; light on 2 Sorghum/leaf; 30 Celsius degree;high light; light on 3 Sorghum/leaf; 30 Celsius degree; low light; lighton 4 Table 71: Provided are the sorghum transcriptome expression sets.

The following parameters were collected by sampling 8-10 plants per plotor by measuring the parameter across all the plants within the plot.

Relative Growth Rate of vegetative dry weight was performed usingFormula VII.

Leaves number—Plants were characterized for leaf number during growingperiod. In each measure, plants were measured for their leaf number bycounting all the leaves of selected plants per plot.

Shoot FW—shoot fresh weight per plant, measurement of all vegetativetissue above ground.

Shoot DW—shoot dry weight per plant, measurement of all vegetativetissue above ground after drying at 70° C. in oven for 48 hours.

The average for each of the measured parameter was calculated and valuesare summarized in Tables 73-76 below. Subsequent correlation analysiswas performed (Table 77). Results were then integrated to the database.

TABLE 72 Sorghum correlated parameters (vectors) Correlated parameterwith Correlation ID Leaves number 1 RGR 2 Shoot DW 3 Shoot FW 4 Table72. Provided are the Sorghum correlated parameters (vectors).

TABLE 73 Measured parameters in Sorghum accessions under 14° C. and lowlight (100 μE m⁻² sec⁻¹) Ecotype/ Treatment Line-1 Line-2 Line-3 Line-4Line-5 Line-6 Line-7 Line-8 Line-9 Line-10 1 3 3 2.75 2.75 2.63 3 3.52.75 2.43 2 2 0.032 −0.01 −0.022 0.024 −0.04 −0.05 0.08 NA −0.1 −0.07 30.041 0.013 0.013 0.009 0.011 0.01 0.03 0.01 0.01 0.01 4 0.55 0.3 0.330.28 0.36 0.36 0.58 0.22 0.18 0.3 Table 73: Provided are the values ofeach of the parameters (as described above) measured in Sorghumaccessions (Seed ID) under 14° C. and low light (100 μE m⁻² sec⁻¹).

TABLE 74 Measured parameters in Sorghum accessions under 30° C. and lowlight (100 μE m⁻² sec⁻¹) Ecotype/ Treatment Line-1 Line-2 Line-3 Line-4Line-5 Line-6 Line-7 Line-8 Line-9 Line-10 1 5.27 5 4.75 4 4 4 5.25 4.53.75 4 2 0.099 0.098 0.09 0.122 0.108 0.08 0.11 0.12 0.04 0.04 3 0.1140.079 0.071 0.056 0.093 0.08 0.04 0.06 0.04 0.05 4 1.35 1.05 0.88 0.951.29 1.13 0.71 0.79 0.67 0.82 Table 74: Provided are the values of eachof the parameters (as described above) measured in Sorghum accessions(Seed ID) under 30° C. and low light (100 μE m⁻² sec⁻¹).

TABLE 75 Measured parameters in Sorghum accessions under 30° C. and highlight (250 μE m⁻² sec⁻¹) Ecotype/ Treatment Line-1 Line-2 Line-3 Line-4Line-5 Line-6 Line-7 Line-8 Line-9 Line-10 1 4 3.7 3.5 3.33 4 4 3.6 3.43.3 3.4 2 0.098 0.096 0.087 0.07 0.094 0.12 0.1 0.1 0.11 0.12 3 0.0760.05 0.047 0.036 0.065 0.09 0.05 0.04 0.04 0.06 4 0.77 0.52 0.49 0.380.71 0.86 0.49 0.45 0.44 0.67 Table 75: Piovided are the values of eachof the parameters (as described above) measured in Sorghum accessions(Seed ID) under 30° C. and high light (250 μE m⁻² sec⁻¹).

TABLE 76 Measured parameters in Sorghum accessions under 14° C. and highlight (250 μE m⁻² sec⁻¹) Ecotype/ Treatment Line-1 Line-2 Line-3 Line-4Line-5 Line-6 Line-7 Line-8 Line-9 Line-10 2 0.053 0.052 0.034 0.040.056 0.06 0.05 0.06 0.07 0.06 3 0.037 0.026 0.021 0.023 0.037 0.04 0.020.02 0.02 0.03 4 0.37 0.25 0.22 0.25 0.43 0.37 0.24 0.23 0.24 0.27 Table76: Provided are the values of each of the parameters (as describedabove) measured in Sorghum accessions (Seed ID) under 14° C. and highlight (250 μE m⁻² sec⁻¹).

TABLE 77 Correlation between the expression level of selected genes ofsome embodiments of the invention in various tissues and the phenotypicperformance Gene Exp. Corr. Gene Exp. Corr. Name R P value set Set IDName R P value set Set ID LYM1196 0.75 1.18E−02 2 4 LYM1196 0.732.61E−02 2 2 LYM1201 0.72 1.07E−01 3 4 LYM1201 0.71 1.15E−01 3 2 LYM12010.77 7.53E−02 3 3 LYM1202 0.81 4.91E−02 3 4 LYM1202 0.86 2.88E−02 3 3LYM1202 0.80 5.87E−02 3 1 LYM1204 0.73 9.87E−02 3 4 LYM1204 0.796.19E−02 3 3 LYM1206 0.82 4.06E−03 2 4 LYM1206 0.72 2.90E−02 2 2 LYM12060.94 5.61E−05 2 3 LYM1208 0.74 9.37E−02 3 4 LYM1208 0.81 5.20E−02 3 3LYM1210 0.75 8.30E−02 3 3 LYM1239 0.77 8.50E−03 4 4 LYM1239 0.779.81E−03 4 3 Table 77. Provided are the correlations (R) between theexpression levels of yield improving genes and their homologues intissues [Leaves or roots; Expression sets (Exp)] and the phenotypicperformance in various biomass, growth rate and/or vigor components[Correlation vector (corr.)] under 14° C. and low light (100 μE m⁻²sec⁻¹) conditions across sorghum accessions. P = p value.

Example 13 Production of Maize Transcriptome and High ThroughputCorrelation Analysis when Grown Under Normal and Defoliation Using 60KMaize Oligonucleotide Micro-Array

To produce a high throughput correlation analysis, the present inventorsutilized a Maize oligonucleotide micro-array, produced by AgilentTechnologies [chem. (dot) agilent (dot) com/Scripts/PDS (dot)asp?1Page=50879]. The array oligonucleotide represents about 60K Maizegenes and transcripts designed based on data from Public databases(Example 1). To define correlations between the levels of RNA expressionand yield, biomass components or vigor related parameters, various plantcharacteristics of 13 different Maize hybrids were analyzed under normaland defoliation conditions. Same hybrids were subjected to RNAexpression analysis. The correlation between the RNA levels and thecharacterized parameters was analyzed using Pearson correlation test[davidmlane (dot) com/hyperstat/A34739 (dot) html].

Experimental Procedures

13 maize hybrids lines were grown in 6 repetitive plots, in field. Maizeseeds were planted and plants were grown in the field using commercialfertilization and irrigation protocols. After silking 3 plots in everyhybrid line underwent the defoliation treatment. In this defoliationtreatment all the leaves above the ear were removed. After the treatmentall the plants were grown according to the same commercial fertilizationand irrigation protocols.

Three tissues at flowering (R1) and grain filling (R3) developmentalstage including leaf (flowering -R1), stem (flowering -R1 and grainfilling -R3), and flowering meristem (flowering -R1) representingdifferent plant characteristics, were sampled from treated and untreatedplants. RNA was extracted as described in “GENERAL EXPERIMENTAL ANDBIOINFORMATICS METHODS”. For convenience, each micro-array expressioninformation tissue type has received a Set ID as summarized in Tables78-79 below.

TABLE 78 Tissues used for Maize transcriptome expression sets (Undernormal conditions) Expression Set Set ID Female meristem at floweringstage under normal conditions 1 leaf at flowering stage under normalconditions 2 stem at flowering stage under normal conditions 3 stem atgrain filling stage under normal conditions 4 Table 78. Provided are theidentification (ID) number of each of the Maize expression sets.

TABLE 79 Tissues used for Maize transcriptome expression sets (Underdefoliation treatment) Expression Set Set ID Female meristem atflowering stage under defoliation treatment 1 leaf at flowering stageunder defoliation treatment 2 stem at flowering stage under defoliationtreatment 3 stem at grain filling stage under defoliation treatment 4Table 79. Provided are the identification (ID) number of each of theMaize expression sets. The following parameters werecollected byimaging.

The image processing system was used, which consists of a personaldesktop computer (Intel P4 3.0 GHz processor) and a public domainprogram—ImageJ 1.37. Java based image processing software, which wasdeveloped at the U.S. National Institutes of Health and is freelyavailable on the internet at rsbweb (dot) nih (dot) gov/. Images werecaptured in resolution of 10 Mega Pixels (3888×2592 pixels) and storedin a low compression JPEG (Joint Photographic Experts Group standard)format. Next, image processing output data for seed area and seed lengthwas saved to text files and analyzed using the JMP statistical analysissoftware (SAS institute).

1000 grain weight—At the end of the experiment all seeds from all plotswere collected and weighed and the weight of 1000 was calculated.

Ear Area (cm²)—At the end of the growing period 5 ears were,photographed and images were processed using the below described imageprocessing system. The Ear area was measured from those images and wasdivided by the number of ears.

Ear Length and Ear Width (cm)—At the end of the growing period 6 earswere, photographed and images were processed using the below describedimage processing system. The Ear length and width (longest axis) wasmeasured from those images and was divided by the number of ears.

Grain Area (cm²)—At the end of the growing period the grains wereseparated from the ear. A sample of ˜200 grains were weight,photographed and images were processed using the below described imageprocessing system. The grain area was measured from those images and wasdivided by the number of grains.

Grain Length and Grain width (cm)—At the end of the growing period thegrains were separated from the ear. A sample of ˜200 grains wereweighted, photographed and images were processed using the belowdescribed image processing system. The sum of grain lengths/or width(longest axis) was measured from those images and was divided by thenumber of grains.

Grain Perimeter (cm)—At the end of the growing period the grains wereseparated from the ear. A sample of ˜200 grains were weight,photographed and images were processed using the below described imageprocessing system. The sum of grain perimeter was measured from thoseimages and was divided by the number of grains.

Ear filed grain area (cm²)—At the end of the growing period 5 ears werephotographed and images were processed using the below described imageprocessing system. The Ear area filled with kernels was measured fromthose images and was divided by the number of Ears.

Filled per Whole Ear—was calculated as the length of the ear with grainsout of the total ear.

Additional parameters were collected either by sampling 6 plants perplot or by measuring the parameter across all the plants within theplot.

Cob width [cm]—The diameter of the cob without grains was measured usinga ruler.

Ear average weight [kg]—At the end of the experiment (when ears wereharvested) total and 6 selected ears per plots were collected. The earswere weighted and the average ear per plant was calculated. The earweight was normalized using the relative humidity to be 0%.

Plant height and Ear height—Plants were characterized for height atharvesting. In each measure, 6 plants were measured for their heightusing a measuring tape. Height was measured from ground level to top ofthe plant below the tassel. Ear height was measured from the groundlevel to the place were the main ear is located.

Ear row number—The number of rows per ear was counted.

Earfresh weight per plant (GF)—During the grain filling period (GF) andtotal and 6 selected ears per plot were collected separately. The earswere weighted and the average ear weight per plant was calculated.

Ears dry weight—At the end of the experiment (when ears were harvested)total and 6 selected ears per plots were collected and weighted. The earweight was normalized using the relative humidity to be 0%.

Ears fresh weight—At the end of the experiment (when ears wereharvested) total and 6 selected ears per plots were collected andweighted.

Ears per plant-number of ears per plant were counted.

Grains weight (Kg.)—At the end of the experiment all ears werecollected. Ears from 6 plants from each plot were separately threshedand grains were weighted.

Grains dry weight (Kg.)—At the end of the experiment all ears werecollected. Ears from 6 plants from each plot were separately threshedand grains were weighted. The grain weight was normalized using therelative humidity to be 0%.

Grain weight per ear (Kg.)—At the end of the experiment all ears werecollected. 5 ears from each plot were separately threshed and grainswere weighted. The average grain weight per ear was calculated bydividing the total grain weight by the number of ears.

Leaves area per plant (GF) and (HD) [LAI]=Total leaf area of 6 plants ina plot. This parameter was measured at two time points during the courseof the experiment; at heading (HD) and during the grain filling period(GF). Measurement was performed using a Leaf area-meter at two timepoints in the course of the experiment; during the grain filling periodand at the heading stage (VT).

Leaves fresh weight (GF) and (HD)—This parameter was measured at twotime points during the course of the experiment; at heading (HD) andduring the grain filling period (GF). Leaves used for measurement of theLAI were weighted.

Lower stem fresh weight (GF) (HD) and (H)—This parameter was measured atthree time points during the course of the experiment: at heading (HD),during the grain filling period (GF) and at harvest (H). Lowerinternodes from at least 4 plants per plot were separated from the plantand weighted. The average internode weight per plant was calculated bydividing the total grain weight by the number of plants.

Lower stem length (GF) (HD) and (H)—This parameter was measured at threetime points during the course of the experiment; at heading (HD), duringthe grain filling period (GF) and at harvest(H). Lower internodes fromat least 4 plants per plot were separated from the plant and theirlength was measured using a ruler. The average internode length perplant was calculated by dividing the total grain weight by the number ofplants.

Lower stem width (GF) (HD) and (H)—This parameter was measured at threetime points during the course of the experiment: at heading (HD), duringthe grain filling period (GF) and at harvest (H). Lower internodes fromat least 4 plants per plot were separated from the plant and theirdiameter was measured using a caliber. The average internode width perplant was calculated by dividing the total grain weight by the number ofplants.

Plant height growth: the RGR of Plant Height was performed using FormulaIII above.

SPAD—Chlorophyll content was determined using a Minolta SPAD 502chlorophyll meter and measurement was performed 64 days post sowing.SPAD meter readings were done on young fully developed leaf. Threemeasurements per leaf were taken per plot. Data were taken after 46 and54 days after sowing (DPS).

Stem fresh weight (GF) and (HD)—This parameter was measured at two timepoints during the course of the experiment: at heading (HD) and duringthe grain filling period (GF). Stems of the plants used for measurementof the LAI were weighted.

Total dry matter—Total dry matter was performed using Formula LXIIabove.

Upper stem fresh weight (GF) (HD) and (H)—This parameter was measured atthree time points during the course of the experiment; at heading (HD),during the grain filling period (.GF) and at harvest (H). Upperinternodes from at least 4 plants per plot were separated from the plantand weighted. The average internode weight per plant was calculated bydividing the total grain weight by the number of plants.

Upper stem length (GF) (HD) and (H)—This parameter was measured at threetime points during the course of the experiment; at heading (HD), duringthe grain filling period (GF) and at harvest(H). Upper internodes fromat least 4 plants per plot were separated from the plant and theirlength was measured using a ruler. The average internode length perplant was calculated by dividing the total grain weight by the number ofplants.

Upper stem width (GF) (HD) and (H) (mm)—This parameter was measured atthree time points during the course of the experiment; at heading (HD),during the grain filling period (GF) and at harvest(.H). Upperinternodes from at least 4 plants per plot were separated from the plantand their diameter was measured using a caliber. The average internodewidth per plant was calculated by dividing the total grain weight by thenumber of plants.

Vegetative dry weight (Kg.)—total weight of the vegetative portion of 6plants (above ground excluding roots) after drying at 70° C. in oven for48 hours weight by the number of plants.

Vegetative fresh weight (Kg.)—total weight of the vegetative portion of6 plants (above ground excluding roots).

Node number—nodes on the stem were counted at the heading stage of plantdevelopment.

TABLE 80 Maize correlated parameters(vectors) under normal conditionsand under defoliation Normal conditions Defoliation Correlated parameterCorrelation Correlated parameter Correlation with ID with ID 1000 grainweight [g] 1 1000 grain weight 1 Cob width [mm] 2 Cob width 2 Ear Area[cm²] 3 Ear Area (cm²) 3 Ear filled grain area 4 Ear filled grain area 4[cm²] [cm²] Ear Width [cm] 5 Ear Width [cm] 5 Ear average weight [g] 6Ear average weight [g] 6 Ear height [cm] 7 Ear height [cm] 7 Ear length[cm] 8 Ear length [cm] 8 Ear row num 9 Ear row num 9 Ears dry weight[kg] 11 Ears dry weight [kg] 10 Ears fresh weight [kg] 12 Ears freshweight [kg] 11 Ear fresh weight per 10 Ears per plant [number] 12 plant(GF) [g/plant] Ears per plant 13 Filled per Whole Ear 13 [number] Grainlength [cm] 16 Grain Perimeter 14 Grain Perimeter [cm] 15 Grain length[cm] 15 Grain width [cm] 17 Grains dry weight 16 Grains dry weight [kg]18 Grains weigh [kg] 17 Grains weight [kg] 19 Grain weight per ear 18[kg] Leaves area per plant 23 Leaves area per plant 20 (GF) [cm²] (hd)[cm²] Leaves area per plant 24 Lower stem fresh 21 (HD) [cm²] weight (H)[g] Grain weight per ear 20 Lower stem fresh 22 [kg] weight (HD) [g]Leaves fresh weight 21 Leaves fresh weight 23 (GF) [g] (HD)[g] Leavesfresh weight 22 Lower stem length (H) 23 (HD) [g] [cm] Leavestemperature 25 Lower stem length 24 (GF) (HD) [cm] Lower stem fresh 26Lower stem width (H) 25 weight (GF) [g] [mm] Lower stem fresh 27 Lowerstem width (HD) 26 weight (H) [g] [mm] Lower stem fresh 28 Node number27 weight(HD) [g] Lower stem length 29 Plant height [cm] 28 (GE) [cm]Lower stem length (H) 30 Plant height growth 29 [cm] [cm/day] Lower stemlength 31 SPAD (GF) [value] 30 (HD) [cm] Lower stem width 32 Stem freshweight (HD) 31 (GF) [mm] [g] Lower stem width (H) 33 Total dry matter[kg] 32 [mm] Lower stem width 34 Upper stem fresh 31 (HD) [mm] weight(H) [g] Node number 35 Upper stem length (H) 34 [cm] Plant height [cm]36 Upper stem width (H) 35 [mm] Plant height growth 37 Vegetative dryweight 36 [cm/day] [kg] Filled per Whole Ear 14 Vegetative fresh weight37 [value] [kg] SPAD (GF)[value] 38 Grain area [cm²] 38 Stem freshweight 39 (GF) [g] Stem fresh weight 40 (HD) [g] Total dry matter [kg]41 Upper stem fresh 42 weight (GF) [g] Upper stem fresh 43 weight (H)[g] Upper stem length 44 (GF) [cm] Upper stem length (H) 45 [cm] Upperstem width (GF) 46 [mm] Upper stem width (H) 47 [mm] Vegetative dryweight 48 [kg] Vegetative fresh 49 weight [kg] Grain area [cm²] 50 Table80.

Thirteen maize varieties were grown, and characterized for parameters,as described above. The average for each parameter was calculated usingthe JMP software, and values are summarized in Tables 81-84 below.Subsequent correlation between the various transcriptome sets for all orsub set of lines was done by the bioinformatic unit and results wereintegrated into the database (Tables 85 and 86 below).

TABLE 81 Measured parameters in Maize Hybrid under normal conditionsEcotype/ Treatment Line-1 Line-2 Line-3 Line-4 Line-5 Line-6 1 296.50263.25 303.61 304.70 281.18 330.45 2 24.63 25.11 23.21 23.69 22.81 22.403 82.30 74.63 77.00 90.15 83.80 96.63 4 80.89 72.42 73.43 85.96 80.6495.03 5 4.66 4.79 4.96 5.00 4.65 4.80 6 209.50 164.63 177.44 218.53205.58 135.77 7 121.67 134.24 149.64 152.14 143.83 133.65 8 22.09 19.6220.02 23.21 22.63 23.74 9 13.00 14.94 14.56 14.56 13.56 13.06 10 351.26323.08 307.87 330.60 320.51 434.60 11 1.26 1.09 1.06 1.31 1.23 1.35 121.69 1.46 1.41 1.70 1.52 1.74 13 1.000 1.111 1.000 1.000 1.000 1.056 140.98 0.97 0.95 0.95 0.95 0.94 15 3.30 3.23 3.28 3.34 3.18 3.38 16 1.1251.123 1.133 1.170 1.081 1.159 17 0.808 0.753 0.789 0.782 0.787 0.823 180.907 0.800 0.766 0.923 0.833 0.986 19 1.04 0.91 0.87 1.06 0.95 1.12 200.15 0.13 0.13 0.15 0.14 0.16 21 230.13 197.64 201.03 205.53 224.81204.49 22 110.97 80.57 157.21 128.83 100.57 111.80 23 7034.60 6402.806353.07 6443.92 6835.50 6507.33 24 4341.25 3171.00 4205.50 4347.503527.00 4517.33 25 33.11 33.52 33.87 34.18 33.78 32.85 26 35.40 25.0326.51 21.74 26.13 34.44 27 23.52 20.34 25.08 14.18 17.53 25.74 28 72.9959.90 74.72 90.48 69.52 66.91 29 19.35 20.40 20.93 21.38 20.03 20.31 3016.76 20.02 22.59 21.68 22.34 21.39 31 14.50 17.75 20.00 19.35 20.3320.75 32 19.86 16.84 16.14 16.37 17.01 17.53 33 19.42 17.19 16.09 16.9217.52 17.88 34 24.14 20.53 20.97 24.43 21.70 19.49 35 15.22 14.56 14.6114.83 15.00 13.83 36 265.11 255.94 271.11 283.89 279.72 268.78 37 5.435.59 6.15 5.99 6.37 6.47 38 59.77 53.17 53.21 54.95 53.99 55.24 39649.03 489.32 524.06 512.66 542.16 627.76 40 758.61 587.88 801.32 794.80721.87 708.38 41 2.57 2.06 2.32 2.44 2.36 2.57 42 19.61 15.54 17.8210.79 14.41 20.31 43 12.94 11.21 12.98 6.50 7.99 12.08 44 16.63 18.7518.38 17.92 17.60 18.79 45 16.93 18.76 18.72 20.01 19.40 19.65 46 16.0014.11 13.50 11.89 13.08 14.34 47 14.93 13.00 12.44 12.04 12.89 13.28 481.31 0.97 1.25 1.13 1.13 1.21 49 3.16 2.75 2.61 2.60 2.42 2.64 50 0.720.67 0.71 0.72 0.67 0.75 Table 81.

TABLE 82 Measured parameters in Maize Hybrid under normal conditions,additional maize lines Ecotype/ Treatment Line-7 Line-8 Line-9 Line-10Line-11 Line-12 Line-13 1 290.88 250.26 306.20 253.19 277.03 269.53274.81 2 23.18 24.88 26.47 23.09 22.69 23.55 26.31 3 78.36 93.91 96.7785.44 76.77 NA 97.99 4 74.41 92.31 95.43 83.28 74.35 NA 96.88 5 4.795.18 5.00 4.95 4.79 NA 5.43 6 147.49 207.11 228.44 215.92 198.69 188.50254.42 7 118.39 145.24 133.78 143.71 134.17 143.00 147.78 8 20.31 22.6023.84 21.74 20.04 NA 22.41 9 16.12 15.89 14.00 15.44 14.89 14.94 16.7810 325.08 327.15 363.70 405.72 338.24 345.32 369.69 11 1.16 1.29 1.371.30 1.19 1.13 1.53 12 1.80 1.60 1.74 1.68 1.56 1.42 1.89 13 1.000 1.0561.000 1.000 1.000 1.000 1.000 14 0.93 0.98 0.99 0.97 0.97 NA 0.99 153.25 3.18 3.29 3.27 3.22 3.15 3.38 16 1.142 1.118 1.151 1.163 1.1241.090 1.206 17 0.740 0.730 0.774 0.739 0.756 0.757 0.760 18 0.820 0.9211.017 0.942 0.852 0.813 1.142 19 0.94 1.05 1.15 1.08 0.97 0.92 1.29 200.14 0.15 0.17 0.16 0.14 0.14 0.19 21 212.41 181.43 199.22 206.91 168.54199.42 200.12 22 116.75 106.95 85.97 102.71 105.73 102.12 143.06 237123.48 6075.21 6597.67 6030.40 6307.06 6617.65 6848.03 24 3984.753696.75 3926.67 3127.67 3942.75 3955.00 4854.00 25 33.19 33.66 33.7832.64 33.95 33.28 33.90 26 27.61 25.26 26.18 34.31 25.50 23.06 25.59 2720.60 16.35 18.90 27.30 22.35 19.26 22.82 28 60.36 63.07 55.89 82.1360.02 58.70 116.12 29 18.08 20.18 19.81 22.89 19.81 19.53 21.40 30 17.0720.69 18.48 23.31 19.39 19.66 19.97 31 15.00 18.68 20.50 22.57 19.8314.50 20.33 32 18.11 17.09 16.87 17.49 16.62 17.10 17.38 33 17.96 18.4217.43 18.07 17.68 17.61 18.93 34 23.47 20.97 21.46 21.41 22.12 23.2524.31 35 14.28 14.72 15.44 14.33 14.44 14.89 14.39 36 244.25 273.56273.22 295.33 259.25 257.89 277.19 37 4.82 6.01 5.99 6.66 5.99 5.62 6.5338 55.38 56.76 55.81 58.54 51.68 55.16 54.16 39 507.78 549.34 509.74662.13 527.43 474.68 544.03 40 660.70 724.58 618.46 837.56 612.81 728.00950.29 41 2.23 2.73 2.33 2.40 2.20 2.08 2.84 42 15.85 14.39 17.85 20.4213.93 13.05 16.45 43 9.72 6.98 9.40 13.58 9.20 7.69 10.17 44 17.07 17.5218.15 18.61 17.69 18.15 18.64 45 16.42 18.34 16.63 19.38 16.71 16.2715.92 46 15.04 13.63 14.73 14.61 13.17 12.77 14.15 47 13.10 13.48 13.4213.27 13.14 12.53 13.79 48 1.07 1.44 0.96 1.10 1.01 0.95 1.31 49 2.222.90 2.22 2.83 2.29 2.15 2.90 50 0.66 0.65 0.70 0.68 0.67 0.65 0.72Table 82.

TABLE 83 Measured parameters in Maize Hybrid under defoliation Eco-type/ Treat- ment Line-1 Line-2 Line-3 Line-4 Line-5 Line-6 1 280.03251.86 294.29 295.36 288.40 308.25 2 19.03 22.12 16.31 21.54 19.84 18.213 53.60 45.50 38.31 58.47 53.89 63.54 4 51.50 42.95 34.59 55.67 51.3661.44 5 4.18 4.21 3.92 4.77 4.51 4.61 6 89.20 100.75 73.39 129.84 129.78115.06 7 119.44 131.56 145.53 156.06 145.28 129.53 8 16.34 13.63 12.8915.94 15.34 17.53 9 12.71 14.36 13.00 14.12 13.47 13.07 10 0.75 0.580.44 0.74 0.78 0.58 11 0.97 0.83 0.63 0.98 1.01 0.80 12 1.000 0.9441.000 0.944 1.000 0.941 13 0.954 0.915 0.873 0.950 0.948 0.961 14 3.1093.144 3.179 3.207 3.196 3.230 15 1.052 1.080 1.079 1.110 1.087 1.094 160.523 0.400 0.289 0.517 0.547 0.398 17 0.604 0.456 0.331 0.588 0.6240.458 18 0.09 0.07 0.05 0.09 0.09 0.08 19 112.27 94.99 125.14 144.48112.50 116.16 20 3914.00 3480.00 4276.50 4985.50 4643.50 4223.00 2123.02 26.50 26.98 15.24 18.19 37.21 22 64.16 53.81 56.41 80.95 71.2766.69 23 16.29 21.44 20.85 22.58 22.94 21.62 24 15.15 18.50 16.67 18.0718.00 19.83 25 19.54 16.90 15.79 17.01 17.12 18.17 26 24.30 20.57 21.0624.87 20.85 20.46 27 15.17 14.39 15.00 15.11 14.50 14.22 28 251.42248.64 268.06 285.11 278.83 261.88 29 6.38 6.32 6.31 6.93 6.83 7.14 3061.21 57.36 58.02 62.36 60.72 62.22 31 713.54 538.04 705.53 803.33703.36 664.23 32 1.54 1.37 1.44 1.53 1.57 1.57 33 8.68 11.08 14.10 4.896.04 13.95 34 16.24 18.83 17.74 19.64 20.74 20.14 35 14.27 12.82 12.6911.09 12.00 13.03 36 0.79 0.78 1.00 0.79 0.79 1.00 37 2.51 1.96 2.802.11 2.20 2.79 38 0.65 0.63 0.67 0.68 0.68 0.68 Table 83.

TABLE 84 Measured parameters in Maize Hybrid under defoliation,additional maize lines Ecotype/ Treatment Line-7 Line-8 Line-9 Line-10Line-11 Line-12 Line-13 1 230.12 271.25 259.43 243.98 262.41 248.64244.16 2 19.77 22.44 20.28 19.64 22.32 23.31 27.78 3 39.83 47.33 65.9043.83 43.28 52.30 58.31 4 36.31 43.34 64.80 39.56 40.43 49.28 55.69 54.10 4.20 4.66 4.06 4.01 4.41 4.98 6 85.04 33.10 161.76 89.36 87.6888.18 124.58 7 123.38 135.00 136.50 136.39 130.32 139.71 143.44 8 13.2114.82 17.60 13.78 13.75 15.53 14.87 9 14.06 13.75 13.94 12.79 13.0014.29 15.83 10 0.45 0.63 0.80 0.54 0.55 0.51 0.75 11 0.65 0.82 1.15 0.880.79 0.69 0.99 12 0.889 1.000 0.882 1.000 1.056 0.944 1.000 13 0.9050.905 0.983 0.890 0.918 0.940 0.950 14 3.130 3.016 3.117 3.086 3.0302.976 3.153 15 1.066 1.024 1.084 1.054 1.025 0.995 1.095 16 0.302 0.4390.667 0.359 0.377 0.344 0.531 17 0.345 0.505 0.767 0.411 0.435 0.3940.609 18 0.06 0.07 0.12 0.06 0.06 0.06 0.09 19 113.78 93.74 89.86 86.98117.27 150.68 161.65 20 3436.00 4593.00 4315.50 4020.50 4154.00 4851.503750.00 21 27.88 17.33 20.51 25.36 28.41 23.16 38.80 22 64.19 76.2357.85 69.98 67.30 72.90 83.58 73 18.76 20.88 17.83 20.70 20.43 20.1124.13 24 16.10 14.83 17.50 23.67 19.00 16.45 20.60 25 18.21 17.23 17.8817.12 17.53 18.63 19.87 26 20.96 22.47 21.23 19.85 21.29 23.58 21.37 2714.39 14.67 15.61 14.39 14.06 14.61 14.00 28 254.64 261.94 268.88 272.71262.50 266.33 279.14 29 6.48 6.28 7.04 7.20 7.34 6.94 7.27 30 59.6559.99 56.76 65.70 57.94 60.31 57.71 31 673.24 738.37 692.23 619.79729.23 794.64 847.52 32 1.34 1.47 1.66 1.48 1.31 1.48 1.71 33 10.93 6.489.01 10.69 10.38 8.49 12.29 34 17.18 19.12 16.74 15.96 17.31 18.19 17.7735 14.25 12.77 13.52 13.08 13.43 13.21 14.72 36 0.88 0.84 0.86 0.94 0.760.96 0.97 37 2.54 2.48 2.35 2.59 2.41 2.70 2.72 38 0.63 0.61 0.62 0.620.60 0.58 0.63 Table 84.

Tables 85 and 86hereinbelow provide the correlations (R) between theexpression levels yield improving genes and their homologues in varioustissues [Expression (Exp) sets] and the phenotypic performance [yield,biomass, growth rate and/or vigor components (Correlation vector (Cor))]under normal and defoliation conditions across maize varieties. P=pvalue.

TABLE 85 Correlation between the expression level of selected genes ofsome embodiments of the invention in various tissues and the phenotypicperformance under normal conditions across maize varieties Corr. Corr.Gene P Exp. Set Gene P Exp. Set Name R value set ID Name R value set IDLYM1129 0.78 4.71E−03 2 35 LYM1130 0.71 6.37E−03 1 31 LYM1130 0.801.70E−03 3 30 LYM1130 0.75 4.96E−03 3 36 LYM1130 0.83 7.75E−04 3 45LYM1131 0.75 3.07E−03 1 19 LYM1131 0.76 2.55E−03 1 20 LYM1131 0.762.55E−03 1 18 LYM1131 0.77 3.35E−03 3 44 LYM1131 0.73 1.15E−02 2 12LYM1131 0.73 1.14E−02 2 16 LYM1131 0.78 4.42E−03 2 42 LYM1132 0.725.76E−03 1 22 LYM1132 0.70 1.54E−02 4 21 LYM1133 0.73 4.91E−03 1 34LYM1133 0.72 5.63E−03 1 33 LYM1133 0.78 7.27E−03 2 8 LYM1134 0.727.90E−03 3 36 LYM1134 0.80 1.82E−03 3 28 LYM1134 0.79 2.15E−03 3 29LYM1134 0.72 1.32E−02 2 40 LYM1134 0.78 4.32E−03 2 44 LYM1134 0.784.59E−03 2 10 LYM1136 0.81 2.76E−03 4 19 LYM1136 0.71 1.38E−02 4 11LYM1136 0.72 1.25E−02 4 12 LYM1136 0.71 1.45E−02 4 16 LYM1136 0.721.30E−02 4 42 LYM1136 0.77 5.84E−03 4 10 LYM1136 0.81 2.66E−03 4 20LYM1136 0.81 2.66E−03 4 18 LYM1136 0.78 4.99E−03 2 17 LYM1138 0.707.61E−03 1 2 LYM1138 0.86 7.58E−04 4 2 LYM1138 0.86 6.79E−04 4 41LYM1138 0.75 1.33E−02 4 4 LYM1138 0.85 1.72E−03 4 14 LYM1138 0.712.27E−02 4 3 LYM1138 0.85 8.98E−04 4 48 LYM1138 0.76 7.00E−03 4 49LYM1138 0.71 1.52E−02 4 20 LYM1138 0.74 9.59E−03 4 33 LYM1138 0.711.52E−02 4 18 LYM1139 0.74 3.81E−03 1 35 LYM1139 0.77 2.26E−03 1 49LYM1139 0.71 9.21E−03 3 15 LYM1139 0.70 1.08E−02 3 48 LYM1139 0.792.32E−03 3 49 LYM1139 0.73 7.07E−03 3 10 LYM1139 0.76 4.00E−03 3 33LYM1139 0.71 1.50E−02 2 24 LYM1139 0.74 8.83E−03 2 48 LYM1139 0.803.33E−03 2 22 LYM1139 0.80 3.31E−03 2 49 LYM1139 0.73 1.15E−02 2 33LYM1140 0.74 5.96E−03 3 40 LYM1140 0.77 3.66E−03 3 28 LYM1140 0.711.36E−02 4 9 LYM1140 0.77 5.11E−03 4 27 LYM1141 0.75 4.93E−03 3 47LYM1141 0.84 6.36E−04 3 32 LYM1141 0.72 7.79E−03 3 38 LYM1141 0.736.91E−03 3 25 LYM1141 0.77 5.30E−03 4 47 LYM1141 0.88 3.56E−04 4 32LYM1141 0.74 8.62E−03 4 40 LYM1142 0.71 6.26E−03 1 40 LYM1142 0.791.46E−03 1 39 LYM1142 0.90 2.36E−05 1 49 LYM1142 0.75 4.79E−03 3 40LYM1142 0.74 9.65E−03 4 19 LYM1142 0.76 6.61E−03 4 11 LYM1142 0.775.42E−03 4 16 LYM1142 0.73 1.55E−02 4 3 LYM1142 0.72 1.87E−02 4 5LYM1142 0.73 1.13E−02 4 20 LYM1142 0.73 1.13E−02 4 18 LYM1143 0.725.66E−03 1 2 LYM1143 0.78 2.91E−03 3 2 LYM1143 0.78 2.75E−03 3 34LYM1143 0.77 3.73E−03 3 28 LYM1143 0.74 9.04E−03 3 14 LYM1143 0.793.50E−03 4 19 LYM1143 0.75 8.05E−03 4 11 LYM1143 0.85 8.59E−04 4 2LYM1143 0.75 8.44E−03 4 41 LYM1143 0.78 8.29E−03 4 4 LYM1143 0.731.01E−02 4 44 LYM1143 0.76 1.15E−02 4 14 LYM1143 0.72 1.89E−02 4 3LYM1143 0.76 6.31E−03 4 29 LYM1143 0.80 3.10E−03 4 20 LYM1143 0.803.10E−03 4 18 LYM1143 0.90 1.55E−04 2 2 LYM1143 0.75 1.22E−02 2 14LYM1143 0.73 1.15E−02 2 23 LYM1146 0.70 1.11E−02 3 49 LYM1146 0.721.30E−02 4 39 LYM1146 0.72 1.34E−02 2 28 LYM1146 0.85 9.22E−04 2 49LYM1149 0.75 3.21E−03 1 45 LYM1149 0.72 7.91E−03 3 41 LYM1149 0.727.94E−03 3 29 LYM1149 0.75 8.28E−03 4 36 LYM1149 0.74 9.89E−03 4 29LYM1149 0.72 1.28E−02 2 19 LYM1149 0.79 4.00E−03 2 11 LYM1149 0.775.42E−03 2 37 LYM1149 0.72 1.18E−02 2 31 LYM1149 0.79 3.49E−03 2 36LYM1149 0.80 5.10E−03 2 4 LYM1149 0.93 8.86E−05 2 8 LYM1149 0.814.88E−03 2 3 LYM1149 0.71 1.42E−02 2 20 LYM1149 0.71 1.47E−02 2 18LYM1151 0.74 8.77E−03 2 26 LYM1151 0.74 8.69E−03 2 42 LYM1152 0.757.98E−03 4 27 LYM1152 0.77 5.84E−03 4 43 LYM1153 0.74 3.76E−03 1 25LYM1153 0.79 3.78E−03 4 13 LYM1153 0.82 2.02E−03 2 13 LYM1153 0.793.52E−03 2 10 LYM1153 0.81 2.52E−03 2 17 LYM1156 0.72 5.33E−03 1 31LYM1156 0.79 1.47E−03 1 42 LYM1156 0.74 6.40E−03 3 37 LYM1156 0.719.50E−03 3 31 LYM1156 0.74 5.73E−03 3 28 LYM1156 0.81 2.33E−03 3 14LYM1156 0.76 4.01E−03 3 29 LYM1156 0.73 1.11E−02 4 37 LYM1156 0.766.59E−03 4 30 LYM1156 0.72 1.25E−02 4 40 LYM1156 0.85 9.53E−04 4 7LYM1156 0.76 6.29E−03 4 28 LYM1156 0.85 8.01E−04 4 29 LYM1158 0.757.41E−03 4 13 LYM1158 0.73 1.08E−02 2 9 LYM1158 0.79 6.25E−03 2 14LYM1158 0.74 1.52E−02 2 5 LYM1159 0.75 2.91E−03 1 33 LYM1162 0.755.37E−03 1 5 LYM1162 0.79 2.37E−03 3 40 LYM1162 0.85 4.62E−04 3 28LYM1162 0.72 1.33E−02 4 19 LYM1162 0.72 1.28E−02 4 11 LYM1162 0.721.26E−02 4 40 LYM1162 0.88 3.26E−04 4 28 LYM1162 0.79 3.89E−03 4 16LYM1162 0.72 1.77E−02 4 5 LYM1162 0.71 1.51E−02 4 22 LYM1162 0.721.29E−02 4 20 LYM1162 0.72 1.29E−02 4 18 LYM1162 0.71 2.04E−02 2 4LYM1163 0.75 4.65E−03 3 21 LYM1164 0.71 1.38E−02 4 13 LYM1164 0.711.49E−02 2 45 LYM1165 0.71 1.47E−02 4 48 LYM1165 0.77 6.02E−03 4 33LYM1165 0.81 4.67E−03 2 5 LYM1167 0.73 6.52E−03 3 19 LYM1167 0.745.94E−03 3 11 LYM1167 0.77 3.16E−03 3 6 LYM1167 0.74 6.04E−03 3 20LYM1167 0.74 6.04E−03 3 18 LYM1167 0.73 1.03E−02 4 29 LYM1167 0.776.07E−03 2 13 LYM1168 0.73 6.50E−03 3 29 LYM1168 0.72 1.27E−02 4 9LYM1169 0.73 6.50E−03 3 32 LYM1169 0.78 4.88E−03 4 2 LYM1169 0.741.45E−02 4 14 LYM1169 0.70 2.30E−02 4 5 LYM1169 0.70 1.58E−02 2 10LYM1170 0.71 6.22E−03 1 47 LYM1170 0.80 9.53E−04 1 32 LYM1171 0.792.12E−03 3 19 LYM1171 0.78 2.49E−03 3 11 LYM1171 0.84 6.86E−04 3 41LYM1171 0.74 6.10E−03 3 28 LYM1171 0.78 3.02E−03 3 49 LYM1171 0.792.07E−03 3 20 LYM1171 0.79 2.07E−03 3 18 LYM1172 0.76 3.79E−03 3 37LYM1172 0.79 3.97E−03 4 37 LYM1172 0.76 6.19E−03 4 31 LYM1172 0.859.93E−04 4 30 LYM1172 0.75 8.09E−03 4 7 LYM1172 0.85 9.04E−04 4 29LYM1172 0.72 1.22E−02 2 11 LYM1172 0.71 1.41E−02 2 36 LYM1172 0.701.63E−02 2 41 LYM1172 0.83 1.43E−03 2 6 LYM1172 0.71 1.42E−02 2 40LYM1172 0.82 1.80E−03 2 28 LYM1172 0.78 4.35E−03 2 29 LYM1172 0.725.52E−03 1 32 LYM1173 0.77 2.21E−03 1 26 LYM1173 0.77 2.09E−03 1 39LYM1173 0.79 1.32E−03 1 38 LYM1174 0.82 5.24E−04 1 32 LYM1174 0.801.73E−03 3 25 LYM1174 0.70 1.55E−02 4 41 LYM1174 0.76 6.75E−03 4 48LYM1175 0.76 1.14E−02 2 8 LYM1175 0.72 1.33E−02 2 1 LYM1176 0.711.41E−02 4 13 LYM1177 0.79 2.17E−03 3 49 LYM1177 0.74 9.40E−03 2 6LYM1177 0.79 3.50E−03 2 40 LYM1178 0.71 1.04E−02 3 27 LYM1178 0.784.91E−03 4 35 LYM1178 0.80 3.23E−03 4 1 LYM1178 0.71 1.50E−02 4 17LYM1178 0.73 1.08E−02 2 35 LYM1179 0.78 2.96E−03 3 2 LYM1179 0.749.45E−03 4 40 LYM1179 0.74 9.46E−03 4 44 LYM1179 0.72 1.21E−02 4 28LYM1180 0.76 2.49E−03 1 38 LYM1180 0.71 9.60E−03 3 19 LYM1180 0.783.06E−03 3 12 LYM1180 0.75 4.80E−03 3 40 LYM1180 0.71 1.01E−02 3 20LYM1180 0.71 1.01E−02 3 18 LYM1181 0.83 4.13E−04 1 47 LYM1181 0.753.44E−03 1 46 LYM1181 0.78 1.71E−03 1 32 LYM1181 0.84 3.68E−04 1 26LYM1181 0.80 1.13E−03 1 39 LYM1181 0.77 2.00E−03 1 42 LYM1181 0.725.17E−03 1 10 LYM1181 0.75 8.47E−03 4 13 LYM1181 0.72 1.33E−02 2 24LYM1181 0.73 1.58E−02 2 8 LYM1181 0.74 8.60E−03 2 1 LYM1181 0.784.83E−03 2 17 LYM1182 0.72 1.19E−02 4 34 LYM1183 0.70 7.25E−03 1 36LYM1183 0.76 2.37E−03 1 29 LMY1184 0.74 6.29E−03 3 9 LYM1184 0.773.64E−03 3 40 LYM1184 0.70 1.07E−02 3 29 LYM1185 0.74 1.53E−02 2 8LYM1186 0.83 4.73E−04 1 9 LYM1186 0.73 1.14E−02 4 33 LYM1187 0.719.84E−03 3 36 LYM1187 0.77 3.18E−03 3 45 LYM1187 0.74 9.25E−03 2 19LYM1187 0.74 8.67E−03 2 11 LYM1187 0.78 4.86E−03 2 40 LYM1187 0.883.16E−04 2 28 LYM1187 0.73 1.13E−02 2 16 LYM1187 0.70 1.54E−02 2 22LYM1187 0.75 8.43E−03 2 20 LYM1187 0.75 8.43E−03 2 18 LYM1163 0.763.76E−03 3 50 LYM1136 0.72 8.76E−03 3 50 LYM1139 0.77 3.27E−03 3 50LYM1153 0.74 9.61E−03 2 50 Table 85.

TABLE 86 Correlation between the expression level of selected genes ofsome embodiments of the invention in various tissues and the phenotypicperformance under defoliation treatment across maize varieties Corr.Corr. Gene P Exp. Set Gene P Exp. Set Name R value set ID Name R valueset ID LYM1129 0.74 5.47E−03 3 14 LYM1129 0.74 9.12E−03 4 24 LYM11310.73 6.61E−03 1 24 LYM1131 0.75 4.82E−03 3 2 LYM1131 0.77 3.54E−03 3 31LYM1131 0.75 4.60E−03 3 2 LYM1131 0.72 1.19E−02 4 26 LYM1133 0.79.86E−03 1 32 LYM1133 0.71 1.01E−02 2 20 LYM1133 0.80 1.63E−03 2 26LYM1134 0.81 1.36E−03 1 30 LYM1134 0.71 1.47E−02 4 10 LYM1137 0.792.35E−03 2 24 LYM1138 0.75 5.34E−03 1 5 LYM1138 0.73 6.75E−03 1 4LYM1138 0.78 2.98E−03 1 18 LYM1138 0.74 5.74E−03 1 17 LYM1138 0.701.11E−02 1 7 LYM1131 0.73 6.74E−03 1 3 LYM1138 0.74 5.63E−03 1 16LYM1138 0.71 1.03E−02 3 24 LYM1138 0.91 3.83E−05 2 26 LYM1139 0.821.02E−03 1 29 LYM1140 0.71 1.03E−02 3 30 LYM1140 0.77 3.14E−03 2 35LYM1140 0.70 1.61E−02 4 2 LYM1140 0.76 6.14E−03 4 9 LYM1140 0.701.54E−02 4 36 LYM1141 0.76 4.05E−03 1 30 LYM1141 0.84 5.61E−04 3 34LYM1141 0.72 1.27E−02 4 34 LYM1142 0.80 1.94E−03 1 27 LYM1142 0.728.41E−03 1 12 LYM1142 0.78 2.61E−03 1 18 LYM1141 0.71 1.05E−02 1 17I.YM1142 0.70 1.05E−02 1 16 LYM1142 0.79 2.09E−03 3 12 LYM1142 0.854.32E−04 2 12 LYM1142 0.70 1.64E−02 4 24 LYM1143 0.80 1.77E−03 3 28LYM1143 0.72 8.59E−03 3 23 LYM1143 0.75 4.76E−03 3 32 LYM1143 0.782.54E−03 3 11 LYM1143 0.81 1.25E−03 3 18 LYM1143 0.87 2.31E−04 3 17LYM1143 0.81 1.27E−03 3 7 LYM1143 0.83 8.99E−04 3 10 LYM1143 0.862.81E−04 3 16 LYM1143 0.71 1.02E−02 2 28 LYM1143 0.71 1.04E−02 2 32LYM1143 0.71 9.11E−03 2 11 LYM1143 0.70 1.12E−02 2 18 LYM1143 0.801.86E−03 2 17 LYM1143 0.81 1.39E−03 2 10 LYM1143 0.79 2.18E−03 2 16LYM1143 0.77 5.51E−03 4 23 LYM1143 0.71 1.44E−02 4 2 LYM1143 0.731.02E−02 4 32 LYM1143 0.72 1.31E−02 4 17 LYM1143 0.71 1.35E−02 4 10LYM1143 0.71 1.51E−02 4 16 LYM1149 0.71 1.04E−02 1 14 LYM1149 0.811.36E−03 1 1 LYM1151 0.75 4.74E−03 1 13 LYM1151 0.71 1.35E−02 4 15LYM1151 0.70 1.59E−02 4 14 LYM1152 0.76 3.77E−03 2 26 LYM1153 0.737.05E−03 1 12 LYM1153 0.84 7.21E−04 1 1 LYM1153 0.74 5.83E−03 2 34LYM1153 0.77 3.17E−03 2 1 LYM1154 0.72 8.27E−03 3 10 LYM1155 0.755.33E−03 1 12 LYM1155 0.72 8.37E−03 2 36 LYM1156 0.74 6.11E−03 1 23LYM1157 0.71 9.33E−03 1 35 LYM1157 0.76 4.42E−03 1 25 LYM1157 0.701.09E−02 1 37 LYM1157 0.79 2.13E−03 2 2 LYM1158 0.79 2.33E−03 1 30LYM1158 0.78 2.67E−03 1 24 LYM1159 0.71 9.95E−03 3 25 LYM1159 0.901.68E−04 4 25 LYM1159 0.72 1.33E−02 4 37 LYM1160 0.78 2.50E−03 1 30LYM1160 0.71 9.64E−03 2 37 LYM1160 0.74 8.60E−03 4 25 LYM1161 0.736.74E−03 1 24 LYM1161 0.71 1.49E−02 4 19 LYM1162 0.73 6.67E−03 1 27LYM1162 0.80 1.64E−03 1 18 LYM1162 0.71 9.53E−03 1 17 LYM1162 0.755.38E−03 1 24 LYM1162 0.71 1.01E−02 1 16 LYM1162 0.81 1.36E−03 3 2LYM1162 0.79 2.49E−03 2 35 LYM1162 0.73 7.54E−03 2 25 LYM1162 0.831.76E−03 4 25 LYM1162 0.79 3.60E−03 4 37 LYM1163 0.77 3.08E−03 1 27LYM1163 0.76 4.45E−03 1 26 LYM1163 0.79 2.12E−03 3 26 LYM1163 0.757.91E−03 4 25 LYM1163 0.73 1.09E−02 4 26 LYM1165 0.79 3.71E−03 4 26LYM1167 0.80 3.05E−03 4 37 LYM1168 0.77 3.24E−03 3 2 LYM1169 0.728.51E−03 2 23 LYM1170 0.72 8.78E−03 1 13 LYM1170 0.77 3.34E−03 1 18LYM1170 0.71 9.63E−03 1 17 LYM1170 0.72 8.03E−03 1 8 LYM1170 0.701.09E−02 1 16 LYM1171 0.71 1.39E−02 4 14 LYM1172 0.79 2.19E−03 1 5LYM1172 0.76 3.95E−03 1 32 LYM1172 0.76 4.43E−03 1 17 LYM1172 0.837.43E−04 1 10 LYM1172 0.76 4.25E−03 1 16 LYM1172 0.76 4.31E−03 3 34LYM1172 0.81 1.51E−03 3 1 LYM1172 0.80 3.03E−03 4 7 LYM1173 0.792.22E−03 3 35 LYM1173 0.91 3.79E−05 3 25 LYM1173 0.74 6.00E−03 3 37LYM1174 0.72 8.01E−03 1 34 LYM1174 0.80 1.97E−03 3 34 LYM1174 0.746.43E−03 2 25 LYM1174 0.84 1.36E−03 4 20 LYM1174 0.74 8.78E−03 4 26LYM1175 0.72 1.33E−02 4 33 LYM1176 0.76 4.34E−03 3 34 LYM1176 0.773.28E−03 3 15 LYM1176 0.80 1.87E−03 3 14 LYM1176 0.85 8.21E−04 4 15LYM1176 0.83 1.67E−03 4 14 LYM1176 0.75 7.91E−03 4 10 LYM1176 0.701.58E−02 4 16 LYM1177 0.81 1.30E−03 3 1 LYM1178 0.74 5.55E−03 3 27LYM1178 0.76 4.43E−03 3 26 LYM1178 0.71 9.14E−03 2 27 LYM1178 0.782.61E−03 2 26 LYM1178 0.87 4.45E−04 4 26 LYM1179 0.71 9.65E−03 3 34LYM1179 0.72 8.50E−03 3 1 LYM1179 0.71 1.01E−02 2 21 LYM1179 0.721.19E−02 4 5 LYM1179 0.73 1.12E−02 4 6 LYM1180 0.72 8.66E−03 1 31LYM1180 0.81 1.25E−03 3 25 LYM1180 0.75 8.39E−03 4 5 LYM1180 0.766.13E−03 4 4 LYM1180 0.88 3.84E−04 4 32 LYM1180 0.77 6.00E−03 4 11LYM1180 0.81 2.26E−03 4 18 LYM1180 0.78 4.58E−03 4 17 LYM1180 0.794.11E−03 4 3 LYM1180 0.75 7.88E−03 4 10 LYM1180 0.79 4.20E−03 4 16LYM1181 0.72 8.26E−03 1 21 LYM1181 0.76 3.87E−03 2 27 LYM1181 0.764.49E−03 2 4 LYM1181 0.72 8.65E−03 2 14 LYM1181 0.76 3.87E−03 2 13LYM1181 0.73 7.16E−03 2 32 LYM1181 0.79 2.27E−03 2 11 LYM1181 0.811.31E−03 2 18 LYM1181 0.85 4.31E−04 2 17 LYM1181 0.76 4.06E−03 2 3LYM1181 0.85 4.63E−04 2 10 LYM1181 0.85 4.20E−04 2 16 LYM1181 0.701.64E−02 4 35 LYM1182 0.76 6.10E−03 4 28 LYM1182 0.76 6.28E−03 4 7LYM1183 0.70 1.05E−02 1 12 LYM1184 0.72 8.95E−03 1 30 LYM1184 0.755.03E−03 1 36 LYM1184 0.70 1.12E−02 1 24 LYM1184 0.82 1.09E−03 3 2LYM1184 0.74 6.31E−03 3 9 LYM1184 0.75 4.65E−03 2 35 LYM1184 0.737.13E−03 2 25 LYM1185 0.78 2.58E−03 1 6 LYM1185 0.70 1.09E−02 3 15LYM1185 0.71 9.38E−03 3 11 LYM1185 0.80 1.87E−03 3 6 LYM1185 0.711.00E−02 3 18 LYM1185 0.76 4.11E−03 2 5 LYM1185 0.79 2.22E−03 2 6LYM1185 0.74 5.70E−03 2 7 LYM1186 0.70 1.07E−02 1 33 LYM1187 0.711.04E−02 2 12 LYM1187 0.78 2.97E−03 2 24 LYM1187 0.71 1.48E−02 4 26LYM1181 0.75 4.54E−03 2 38 LYM1129 0.73 6.75E−03 3 38 LYM1130 0.745.60E−03 2 38 LYM1134 0.79 4.01E−03 4 38 LYM1141 0.72 7.70E−03 3 38LYM1149 0.81 1.45E−03 1 38 LYM1154 0.75 4.59E−03 3 38 LYM1171 0.784.78E−03 4 38 LYM1172 0.72 7.84E−03 3 38 LYM1174 0.74 5.77E−03 3 38LYM1176 0.82 1.13E−03 3 38 LYM1176 0.77 5.93E−03 4 38 LYM1177 0.719.47E−03 1 38 Table 86.

Example 14 Production of Barley Transcriptome and High ThroughputCorrelation Analysis Using 60K Barley Oligonucleotide Micro-Array

In order to produce a high throughput correlation analysis, the presentinventors utilized a Barley oligonucleotide micro-array, produced byAgilent Technologies [chem. (dot) agilent (dot) com/Scripts/PDS (dot)asp?1Page=50879]. The array oligonucleotide represents about 33.777Barley genes and transcripts. In order to define correlations betweenthe levels of RNA expression and yield or vigor related parameters,various plant characteristics of 55 different Barley accessions wereanalyzed. Same accessions were subjected to RNA expression analysis. Thecorrelation between the RNA levels and the characterized parameters wasanalyzed using Pearson correlation test [davidmlane (dot)com/hyperstat/A34739 (dot) html].

Experimental Procedures Four tissues at different developmental stages[leaf, flag leaf, spike and peduncle].

representing different plant characteristics, were sampled and RNA wasextracted as described hereinabove under “GENERAL EXPERIMENTAL ANDBIOINFORMATICS METHODS”.

For convenience, each micro-array expression information tissue type hasreceived a Set ID as summarized in Table 87 below.

TABLE 87 Barley transcriptome expression sets Expression Set Set ID Flagleaf at booting stage under normal conditions 1 Spike at grain fillingstage under normal conditions 2 Spike at booting stage under normalconditions 3 Stem at booting stage under normal conditions 4 Table 87:Provided are the identification (ID) letters of each of the Barleyexpression sets

Barley yield components and vigor related parameters assessment—55Barley accessions in 5 repetitive blocks (named A, B. C. D and E), eachcontaining 48 plants per plot were grown in field. Plants werephenotyped on a daily basis. Harvest was conducted while 50% of thespikes were dry to avoid spontaneous release of the seeds. All materialwas oven dried and the seeds were threshed manually from the spikesprior to measurement of the seed characteristics (weight and size) usingscanning and image analysis. The image analysis system included apersonal desktop computer (Intel P4 3.0 GHz processor) and a publicdomain program—ImageJ 1.37 (Java based image processing program, whichwas developed at the U.S. National Institutes of Health and freelyavailable on the internet [rsbweb (dot) nih (dot) gov/]. Next, analyzeddata was saved to text files and processed using the JMP statisticalanalysis software (SAS institute).

At the end of the experiment (50% of the spikes were dry) all spikesfrom plots within blocks A-E were collected, and the followingmeasurements were performed:

% reproductive tiller percentage—The percentage of reproductive tillersat flowering was performed using Formula XXVI above.

1000 grain weight (gr)—At the end of the experiment all grains from allplots were collected and weighted and the weight of 1000 werecalculated.

Average seedling dry weight (gr)—Weight of seedling after drying/numberof plants.

Average shoot dry weight (gr)—Weight of Shoot at flowering stage afterdrying/number of plants.

Average spike weight (g)—Calculate spikes dry weight after drying at 70°C. in oven for 48 hours, at harvest/num of spikes.

Average spike dry weight per plant (g)—At the end of the experiment thebiomass and spikes weight of each plot was separated, measured anddivided by the number of plants.

Vegetative dry weight (g)—Total weight of the vegetative portion aboveground (excluding roots) after drying at 70° C. in oven for 48 hours.The biomass weight of each plot was measured and divided by the numberof plants.

Field spike length (cm)—Measure spike length without the Awns atharvest.

Grain Area (cm²)—A sample of ˜200 grains were weighted, photographed andimages were processed using the below described image processing system.The grain area was measured from those images and was divided by thenumber of grains.

Grain Length and Grain width (cm)—A sample of ˜200 grains was weighted,photographed and images were processed using the below described imageprocessing system. The sum of grain lengths and width (longest axis) wasmeasured from those images and was divided by the number of grains.

Grain Perimeter (cm)—A sample of ˜200 grains were weight, photographedand images were processed using the below described image processingsystem. The sum of grain perimeter was measured from those images andwas divided by the number of grains.

Grains per spike—The total number of grains from 5 spikes that weremanually threshed was counted. The average grain per spike wascalculated by dividing the total grain number by the number of spikes.

Grain yield per plant (gr)—The total grains from 5 spikes that weremanually threshed were weighted. The grain yield was calculated bydividing the total weight by the plants number.

Grain yield per spike (gr)—The total grains from 5 spikes that weremanually threshed were weighted. The grain yield was calculated bydividing the total weight by the spike number.

Growth habit scoring—At growth stage 10 (booting), each of the plantswas scored for its growth habit nature. The scale that was used was 1for prostate nature till 9 for erect.

Harvest Index (for Barley)—The harvest index was performed using FormulaXVIII (above).

Number days to anthesis—Calculated as the number of days from sowingtill 50% of the plot arrive anthesis.

Number days to maturity—Calculated as the number of days from sowingtill 50% of the plot arrive maturity.

Plant height—At harvest stage (50% of spikes were dry), each of theplants was measured for its height using measuring tape. Height wasmeasured from ground level to top of the longest spike excluding awns.

Reproductive period—Calculate number of days from booting to maturity.

Reproductive tillers number—Number of Reproductive tillers with flagleaf at flowering.

Relative Growth Rate—RGR of vegetative dry weight was performed usingFormula VII above.

Spike area (cm²)—At the end of the growing period 5 ‘spikes’ werephotographed and images were processed using the below described imageprocessing system. The ‘spike’ area was measured from those images andwas divided by the number of ‘spikes’.

Spike length and width analysis—At the end of the experiment the lengthand width of five chosen spikes per plant were measured using measuringtape excluding the awns.

Spike max width—Measured by imaging the max width of 10-15 spikesrandomly distributed within a pre-defined 0.5m2 of a plot. Measurementswere carried out at the middle of the spike.

Spikes Index—The Spikes index was performed using Formula XXVII above.

Spike number analysis—The spikes per plant were counted at harvest.

No. of tillering—tillers were counted per plant at heading stage (meanper plot).

Total dry mater per plant—Calculated as Vegetative portion above groundplus all the spikes dry weight per plant.

TABLE 88 Barley correlated parameters (vectors) Correlation Correlatedparameter with ID % reproductive tiller percentage (%) 1 1000 grainweight (gr) 2 Average spike dry weight per plant (H) (g) 3 Averagevegetative dry weight per plant (H) (g) 4 Average shoot dry weight (H)(gr) 5 Average spike weight (H) (g) 6 Grain Perimeter (cm) 7 Grain Area(cm²) 8 Grain Length (cm) 9 Grain width (cm) 10 Grains per spike(number) 11 Grain yield per plant (gr) 12 Grain yield per spike (gr) 13Growth habit (scores 1-9) 14 Harvest Index (value) 15 Number days toanthesis (days) 16 Number days to maturity (days) 17 Plant height (cm)18 RGR 19 Reproductive period (days) 20 Reproductive tillers number (F)(number) 21 Spike area (cm²) 22 Spike length (cm) 23 Spike width (cm) 24Spike max width (cm) 25 Spike index (cm) 26 Spikes per plan (numbers) 27Tillering (Heading) (number) 28 Total dry matter per plant (kg) 29Average seedling dry weight (gr) 30 Field spike length (cm) 31 Table 88.Provided are the Barley correlated parameters (vectors).

Experimental Results

55 different Barley accessions were grown and characterized for 31parameters as described above. Among the 55 lines and ecotypes, 27 areHordeum spontaneum and 19 are Hordeum vulgare. The average for each ofthe measured parameters of all Barley accessions was calculated usingthe JMP software and values are summarized in Tables 89-96 below.Subsequent correlation analysis across all 55 lines and ecotypes betweenthe various transcriptome expression sets (Table 87) and the averageparameters was conducted and results were integrated to the database(Table 104 below). Tables 97-100 show phenotypic data of Hordeumspontaneum lines and ecotypes, and the correlation data between thevarious transcriptome expression sets (Table 87) and the averageparameters is shown in Table 105 below. Tables 101-103 show phenotypicdata of Hordeum vulgare lines and ecotypes, and the correlation databetween the various transcriptome expression sets (Table 87) and theaverage parameters is shown in Table 106 below.

TABLE 89 Measured parameters of correlation IDs in Barley accessionsEco- type/ Treat- ment Line-1 Line-2 Line-3 Line-4 Line-5 Line-6 Line-71 4.31 18.25 9.16 40.15 33.22 NA 7.86 2 50.11 49.98 31.77 52.43 47.2249.33 53.02 3 80.88 60.47 36.42 69.45 61.03 63.22 88.26 4 46.33 85.0382.74 127.37 79.51 82.95 68.92 5 11.34 52.57 48.28 126.89 60.56 NA 31.406 3.33 1.56 2.37 3.11 3.18 2.85 3.37 7 2.62 2.41 2.31 2.67 2.62 2.592.59 8 0.30 0.28 0.24 0.30 0.29 0.29 0.30 9 1.09 0.97 0.92 1.07 1.091.07 1.05 10 0.40 0.41 0.35 0.41 0.39 0.39 0.41 11 56.51 21.05 45.1644.35 47.12 43.51 55.88 12 64.98 37.47 NA 51.69 49.15 46.44 NA 13 2.911.02 1.37 2.33 2.23 2.14 2.85 14 4.20 1.00 1.40 2.60 2.60 1.00 2.60 150.51 0.25 NA 0.26 0.35 0.32 NA 16 90.80 124.40 122.00 NA 122.00 NA102.60 17 148.00 170.00 157.00 170.00 167.40 170.00 158.80 18 83.9779.87 99.04 122.46 108.03 87.00 97.03 19 2.45 3.96 3.91 4.75 4.12 NA3.24 20 57.20 45.60 35.00 NA 48.00 NA 56.20 21 1.00 9.20 5.00 19.2014.63 NA 2.80 22 9.90 7.82 9.68 11.07 10.17 9.98 9.94 23 9.49 10.26 7.887.97 8.42 8.12 7.61 24 1.23 0.87 1.44 1.68 1.47 1.51 1.57 25 1.41 1.051.59 1.79 1.60 1.61 1.70 26 0.64 0.42 0.30 0.35 0.44 0.43 0.56 27 45.2756.27 31.50 32.42 35.40 36.73 36.93 28 24.00 48.70 52.00 47.60 45.00 NA35.20 29 127.22 145.50 119.15 196.82 140.54 146.17 157.18 30 0.053 0.0590.044 0.051 0.053 0.047 0.066 31 9.57 NA 7.66 7.93 8.13 NA 7.21 Table89. Provided are the values of each of the parameters measured in Barleyaccessions (1-7) according to the correlation identifications (see Table88).

TABLE 90 Barley accessions, additional measured parameters Ecotype/Treatment Line-8 Line-9 Line-10 Line-11 Line-12 Line-13 Line-14 1 16.675.64 5.29 18.34 4.03 8.83 4.82 2 61.33 49.97 51.70 56.46 53.97 50.3656.81 3 91.89 99.05 66.99 60.22 87.61 71.76 76.71 4 82.88 56.80 64.1454.23 73.23 49.50 47.63 5 44.57 9.71 38.18 46.74 42.32 11.62 9.33 6 4.133.47 3.15 1.88 3.35 3.60 3.24 7 2.78 2.66 2.63 2.28 2.54 2.37 2.71 80.33 0.29 0.30 0.28 0.30 0.27 0.32 9 1.15 1.09 1.08 0.88 1.03 0.96 1.1210 0.42 0.39 0.39 0.45 0.42 0.41 0.41 11 58.33 56.03 59.08 27.27 55.8761.53 50.80 12 78.18 79.86 54.34 46.37 71.89 56.24 61.63 13 3.47 2.602.84 1.51 2.84 2.98 2.85 14 1.00 5.00 3.00 1.00 1.00 2.20 3.00 15 0.450.51 0.41 0.40 0.45 0.48 0.50 16 111.60 86.80 106.20 117.80 111.60 85.4090.00 17 156.20 159.60 157.00 162.20 159.60 157.00 150.50 18 104.0170.78 98.11 57.88 94.52 73.20 78.65 19 3.82 2.30 3.60 3.83 3.63 2.432.26 20 44.60 72.80 50.80 44.40 46.00 71.60 61.50 21 6.30 1.20 2.1010.00 2.60 1.63 1.00 22 9.89 9.58 11.19 8.76 10.49 10.83 11.23 23 6.397.73 8.45 10.55 7.60 7.87 9.42 24 1.83 1.50 1.57 0.96 1.63 1.63 1.43 251.93 1.59 1.71 1.17 1.75 1.72 1.58 26 0.51 0.64 0.51 0.53 0.55 0.61 0.6227 32.10 48.53 29.80 50.80 32.40 26.80 42.42 28 38.50 21.50 36.10 57.2542.20 19.13 21.63 29 178.63 155.85 131.13 114.45 160.84 121.26 124.34 300.052 0.062 0.051 0.062 0.060 0.056 0.045 31 5.65 7.94 8.55 10.59 7.447.36 9.60 Table 90. Provided are the values of each of the parametersmeasured in Barley accessions (8-14) according to the correlationidentifications (see Table 88).

TABLE 91 Barley accessions, additional measured parameters Ecotype/Treatment Line-15 Line-16 Line-17 Line-18 Line-19 Line-20 Line-21 129.49 5.01 3.74 11.42 5.13 4.07 6.62 2 57.98 51.44 58.07 53.45 48.6639.48 41.96 3 81.14 77.90 68.17 70.73 54.13 48.72 64.51 4 66.51 77.5081.58 67.92 81.05 66.73 91.79 5 47.56 30.93 NA 35.49 38.41 NA 41.56 63.12 1.69 1.66 3.50 1.16 2.95 1.36 7 2.90 2.28 2.42 2.65 2.16 2.16 2.458 0.34 0.27 0.30 0.30 0.26 0.23 0.23 9 1.22 0.89 0.96 1.08 0.83 0.850.94 10 0.41 0.42 0.44 0.40 0.42 0.39 0.36 11 45.48 24.77 21.15 59.7217.46 63.19 19.87 12 64.82 56.43 49.68 54.97 40.33 NA NA 13 2.39 1.211.18 2.93 0.83 2.38 0.78 14 1.00 1.00 3.80 3.80 1.00 3.40 1.00 15 0.440.36 0.33 0.40 0.29 NA NA 16 113.20 113.40 98.50 109.60 119.40 98.80119.40 17 158.00 170.00 170.00 155.20 170.00 156.20 170.00 18 90.7364.2.7 82.73 94.12 63.47 102.12 94.80 19 3.89 3.46 NA 3.60 3.64 NA 3.7420 44.80 56.60 71.50 45.60 50.60 57.40 50.60 21 17.00 3.00 1.00 3.804.20 1.00 4.63 22 7.89 9.15 8.57 11.30 7.04 8.37 7.28 23 6.68 12.0510.74 8.60 8.94 6.03 10.99 24 1.45 0.88 0.92 1.56 0.92 1.67 0.76 25 1.521.03 1.10 1.72 1.08 1.75 0.90 26 0.55 0.50 0.45 0.51 0.39 0.42 0.41 2739.73 71.33 65.40 33.27 82.47 32.87 73.13 28 59.80 62.50 31.20 34.0078.90 26.50 69.88 29 147.66 155.41 149.76 138.64 135.18 115.45 156.29 300.051 0.037 0.047 0.043 0.056 0.053 0.055 31 6.23 NA NA 8.57 NA 6.26 NATable 91. Provided are the values of each of the parameters measured inBarley accessions (15-21) according to the correlation identificationssee Table 88).

TABLE 92 Barley accessions, additional measured parameters Eco- type/Treat- Line- Line- Line- Line- Line- Line- Line- ment 22 23 24 25 26 2728 1 3.51 7.34 31.12 NA NA 11.07 21.67 2 18.60 42.65 39.67 24.43 28.4228.40 23.52 3 33.60 33.22 52.10 33.28 47.69 52.78 52.54 4 50.17 45.2267.20 43.40 79.51 61.09 59.71 5 174.82 8.39 51.83 NA NA 38.46 38.79 60.90 3.09 1.22 0.91 0.92 1.08 0.95 7 2.65 2.19 2.44 2.90 2.62 2.66 2.688 0.25 0.25 0.25 0.27 0.25 0.25 0.24 9 1.11 0.88 0.96 1.20 1.07 1.081.11 10 0.31 0.39 0.37 0.32 0.32 0.33 0.31 11 16.30 60.49 17.54 12.0020.01 20.00 17.01 12 NA NA NA NA NA NA NA 13 0.31 2.43 0.67 0.31 0.560.56 0.38 14 1.00 3.00 1.00 1.00 1.00 1.00 1.00 15 NA NA NA NA NA NA NA16 95.60 90.00 111.00 83.60 122.00 111.40 109.20 17 133.00 161.40 145.80140.20 153.00 143.00 140.40 18 90.49 88.53 90.10 92.47 99.08 91.69 94.6719 5.01 2.12 3.97 NA NA 3.67 3.68 20 37.40 71.40 34.80 56.60 31.00 31.6031.20 21 1.88 1.00 15.50 NA NA 7.10 15.70 22 4.98 11.56 6.52 5.39 8.168.08 5.73 23 8.58 9.02 8.63 7.96 10.20 10.52 8.35 24 0.68 1.53 0.88 0.810.97 0.92 0.78 25 0.79 1.68 1.01 0.88 1.05 1.01 0.90 26 0.41 0.42 0.440.44 0.38 0.46 0.47 27 88.07 20.53 48.53 51.33 65.80 55.80 65.60 2855.25 14.00 48.50 NA NA 69.00 76.40 29 83.76 78.44 119.30 76.68 127.20113.88 112.25 30 0.027 0.058 0.047 0.048 0.044 0.045 0.046 31 9.74 9.068.69 8.90 10.13 10.61 9.60 Table 92. Provided are the values of each ofthe parameters measured in Barley accessions (22-28) according to thecorrelation identifications (see Table 88).

TABLE 93 Barley accessions, additional measured parameters Ecotype/Treatment Line-29 Line-30 Line-31 Line-32 Line-33 Line-34 Line-35 1 3.9316.51 3.19 10.55 26.51 15.15 4.28 2 45.71 26.47 23.14 27.64 29.37 27.7442.12 3 83.98 47.05 48.92 47.26 48.82 46.56 89.21 4 45.36 60.37 67.3967.12 61.35 59.03 71.31 5 10.63 29.60 14.28 37.74 39.23 34.46 41.16 62.99 0.85 0.85 0.89 1.10 1.09 2.93 7 2.77 2.66 2.57 2.93 3.16 2.99 2.978 0.30 0.25 0.24 0.29 0.33 0.29 0.30 9 1.13 1.09 1.06 1.23 1.33 1.271.16 10 0.39 0.32 0.32 0.33 0.34 0.32 0.39 11 56.85 18.19 13.50 12.8314.49 13.72 54.84 12 NA NA NA NA NA NA NA 13 2.63 0.46 0.31 0.37 0.430.39 2.14 14 2.20 1.00 1.00 1.00 1.00 1.00 1.00 15 NA NA NA NA NA NA NA16 89.20 104.00 89.20 97.80 113.60 109.20 110.40 17 151.60 140.20 140.40140.40 145.80 143.00 156.20 18 66.65 105.78 112.21 103.83 105.74 107.45100.65 19 2.37 3.42 2.67 3.64 3.65 3.51 3.74 20 62.40 36.20 51.20 42.6032.20 33.80 45.80 21 1.00 12.30 1.10 8.50 18.67 11.00 2.50 22 8.94 4.695.47 5.92 6.16 6.88 11.03 23 7.75 6.85 8.51 8.32 9.80 9.28 8.77 24 1.370.81 0.75 0.83 0.74 0.88 1.53 25 1.52 0.91 0.85 0.96 0.82 0.94 1.60 260.65 0.44 0.42 0.41 0.41 0.44 0.56 27 44.87 77.13 85.00 67.53 50.8755.67 38.60 28 26.50 76.60 35.30 75.30 68.50 66.80 55.80 29 129.34107.42 116.31 114.38 104.47 105.59 160.52 30 0.049 0.037 0.044 0.0470.042 0.056 0.051 31 7.97 8.24 9.14 8.71 9.82 10.00 8.47 Table 93.Provided are the values of each of the parameters measured in Barleyaccessions (29-35) according to the correlation identifications (seeTable 88).

TABLE 94 Barley accessions, additional measured parameters Ecotype/Treatment Line-36 Line-37 Line-38 Line-39 Line-40 Line-41 Line-42 1 9.464.75 NA 4.60 21.49 21.20 14.49 2 26.38 19.78 31.00 47.79 32.57 36.8924.24 3 43.46 27.36 44.56 69.91 44.21 50.54 44.04 4 48.56 31.46 59.3143.15 72.36 91.79 63.37 5 23.84 11.91 NA 8.31 55.42 55.88 31.34 6 0.741.15 1.32 3.51 1.45 1.40 0.93 7 3.17 2.74 2.69 2.93 2.38 2.67 3.05 80.30 0.26 0.26 0.32 0.23 0.28 0.30 9 1.30 1.11 1.10 1.21 0.95 1.09 1.2810 0.31 0.32 0.33 0.39 0.33 0.36 0.33 11 11.25 16.11 21.71 58.20 34.1920.75 11.50 12 NA NA NA NA NA NA NA 13 0.24 0.32 0.66 2.82 0.94 0.750.31 14 1.00 1.00 1.00 3.80 1.00 1.40 1.00 15 NA NA NA NA NA NA NA 16108.40 91.60 115.60 84.20 118.00 116.80 111.00 17 140.40 133.00 145.80148.00 153.80 144.20 140.20 18 106.34 78.29 107.63 77.57 93.91 126.08107.15 19 3.17 2.50 NA 2.12 4.03 NA 3.44 20 32.00 41.40 30.20 63.8036.00 27.40 29.25 21 7.40 1.50 NA 0.81 14.80 15.50 10.70 22 5.17 7.728.37 7.41 7.83 8.38 5.09 23 7.81 11.96 11.32 7.52 8.33 10.12 8.27 240.79 0.75 0.86 1.16 1.15 0.99 0.72 25 0.91 0.92 0.94 1.31 1.24 1.06 0.8226 0.47 0.48 0.43 0.62 0.37 0.36 0.41 27 64.67 50.93 48.40 32.00 43.4045.80 73.53 28 69.30 32.20 NA 15.81 66.40 75.13 71.20 29 92.02 58.82110.88 113.06 116.57 149.88 107.41 30 0.056 0.033 0.036 0.062 0.049 NA0.057 31 8.36 12.49 11.03 8.21 7.97 10.44 8.66 Table 94. Provided arethe values of each of the parameters measured in Barley accessions(36-42) according to the correlation identifications (see Table 88).

TABLE 95 Barley accessions, additional measured parameters Ecotype/Treatment Line-43 Line-44 Line-45 Line-46 Line-47 Line-48 Line-49 117.05 12.52 9.87 10.75 10.80 14.99 16.12 2 27.81 23.34 31.77 27.36 25.7024.92 26.31 3 50.12 40.37 55.92 33.55 31.74 50.70 44.59 4 69.41 58.5161.56 42.29 41.24 71.38 73.03 5 32.88 35.99 42.56 19.47 26.16 39.2249.89 6 0.96 0.82 1.34 1.16 1.18 0.94 1.05 7 2.77 2.94 3.18 3.06 2.752.62 2.99 8 0.26 0.29 0.33 0.30 0.26 0.24 0.29 9 1.14 1.25 1.32 1.251.13 1.06 1.25 10 0.33 0.32 0.35 0.34 0.32 0.32 0.33 11 17.55 10.6515.98 14.58 17.44 18.90 14.60 12 NA NA NA NA NA NA NA 13 0.47 0.25 0.530.43 0.45 0.47 0.40 14 1.00 1.00 1.00 1.00 1.00 1.00 1.00 15 NA NA NA NANA NA NA 16 111.00 111.00 111.00 99.20 105.80 111.00 117.20 17 146.00140.20 143.00 133.00 133.00 143.00 148.20 18 106.69 96.26 99.81 91.7580.80 105.57 101.87 19 3.52 3.60 3.75 2.94 3.29 3.68 3.84 20 35.00 29.2032.00 33.80 27.20 32.00 31.00 21 15.00 11.70 6.90 5.50 10.30 12.40 13.3322 5.03 4.88 8.33 7.43 6.71 6.61 7.10 23 8.45 7.95 10.21 11.52 10.179.09 9.79 24 0.65 0.72 0.96 0.76 0.77 0.94 0.85 25 0.76 0.82 1.04 0.910.92 0.97 0.95 26 0.42 0.41 0.48 0.44 0.46 0.42 0.38 27 79.33 61.6749.13 55.10 56.67 62.20 70.93 28 86.70 90.70 71.40 58.50 90.90 87.50108.50 29 119.53 98.88 117.49 75.84 72.98 122.08 117.62 30 0.040 0.0340.048 0.045 0.039 0.065 0.046 31 9.91 8.51 10.18 11.82 10.58 9.42 10.04Table 95. Provided are the values of each of the parameters measured inBarley accessions (43-49) according to the correlation identifications(see Table 88).

TABLE 96 Barley accessions, additional measured parametersEcotype/Treatment Line-50 Line-51 Line-52 Line-53 Line-54 Line-55 131.13 NA 15.51 6.88 7.07 6.72 2 30.08 24.82 26.46 21.49 43.66 47.91 336.91 26.20 57.49 47.76 43.70 68.61 4 50.75 52.91 73.30 65.81 56.28 NA 537.91 NA 38.72 29.92 14.62 67.47 6 1.01 1.01 0.84 0.75 3.71 2.78 7 3.063.24 2.90 2.65 2.24 2.56 8 0.31 0.32 0.26 0.25 0.25 0.28 9 1.26 1.361.17 1.10 0.88 1.05 10 0.35 0.33 0.32 0.31 0.40 0.38 11 13.58 13.0719.84 17.16 65.39 43.77 12 NA NA NA NA 34.58 53.97 13 0.40 0.32 0.500.38 2.64 2.06 14 1.00 1.00 1.00 1.00 5.00 1.80 15 NA NA NA NA 0.35 NA16 113.00 122.60 111.00 107.60 88.40 128.00 17 143.60 152.00 142.40140.40 157.00 170.00 18 95.35 80.26 105.02 98.42 93.79 90.30 19 NA NA3.66 3.41 2.18 4.23 20 30.60 29.40 31.40 32.80 68.60 42.00 21 20.20 NA18.30 6.60 2.50 3.10 22 6.86 8.62 7.16 5.75 10.74 10.04 23 9.38 11.7310.01 8.78 8.54 8.59 24 0.87 0.87 0.86 0.77 1.49 1.45 25 0.94 0.97 0.940.89 1.68 1.57 26 0.42 0.33 0.44 0.42 0.44 NA 27 39.27 45.00 74.58 74.5320.80 38.00 28 64.60 NA 113.50 95.60 15.60 43.20 29 87.66 79.11 130.79113.58 99.98 NA 30 NA 0.040 0.040 0.045 0.053 0.054 31 9.41 11.67 10.609.72 8.26 9.22 Table 96. Provided are the values of each of theparameters measured in Barley accessions (50-55) according to thecorrelation identifications (see Table 88).

TABLE 97 Measured parameters of correlation Ids in Barley Hordeumspontaneum accessions Ecotype/ Treatment Line-21 Line-22 Line-24 Line-25Line-26 Line-27 Line-28 1 6.62 3.51 31.12 NA NA 11.07 21.67 2 41.9618.60 39.67 24.43 28.42 28.40 23.52 3 64.51 33.60 52.10 33.28 47.6952.78 52.54 4 91.79 50.17 67.20 43.40 79.51 61.09 59.71 5 41.56 174.8251.83 NA NA 38.46 38.79 6 1.36 0.90 1.22 0.91 0.92 1.08 0.95 7 2.45 2.652.44 2.90 2.62 2.66 2.68 8 0.23 0.25 0.25 0.27 0.25 0.25 0.24 9 0.941.11 0.96 1.20 1.07 1.08 1.11 10 0.36 0.31 0.37 0.32 0.32 0.33 0.31 1119.87 16.30 17.54 12.00 20.01 20.00 17.01 12 NA NA NA NA NA NA NA 130.78 0.31 0.67 0.31 0.56 0.56 0.38 14 1.00 1.00 1.00 1.00 1.00 1.00 1.0015 NA NA NA NA NA NA NA 16 119.40 95.60 111.00 83.60 122.00 111.40109.20 17 170.00 133.00 145.80 140.20 153.00 143.00 140.40 18 94.8090.49 90.10 92.47 99.08 91.69 94.67 19 3.74 5.01 3.97 NA NA 3.67 3.68 2050.60 37.40 34.80 56.60 31.00 31.60 31.20 21 4.63 1.88 15.50 NA NA 7.1015.70 22 7.28 4.98 6.52 5.39 8.16 8.08 5.73 23 10.99 8.58 8.63 7.9610.20 10.52 8.35 24 0.76 0.68 0.88 0.81 0.97 0.92 0.78 25 0.90 0.79 1.010.88 1.05 1.01 0.90 26 0.41 0.41 0.44 0.44 0.38 0.46 0.47 27 73.13 88.0748.53 51.33 65.80 55.80 65.60 28 69.88 55.25 48.50 NA NA 69.00 76.40 29156.29 83.76 119.30 76.68 127.20 113.88 112.25 30 0.055 0.027 0.0470.048 0.044 0.045 0.046 31 NA 9.74 8.69 8.90 10.13 10.61 9.60 Table 97.Provided are the values of each of the parameters measured in BarleyHordeum spontaneum accessions (21-22, 24-28) according to thecorrelation identifications (see Table 88).

TABLE 98 Measured parameters of correlation Ids in Barley Hordeumspontaneum accessions Ecotype/ Treatment Line-30 Line-31 Line-32 Line-33Line-34 Line-36 Line-37 1 16.51 3.19 10.55 26.51 15.15 9.46 4.75 2 26.4723.14 27.64 29.37 27.74 26.38 19.78 3 47.05 48.92 47.26 48.82 46.5643.46 27.36 4 60.37 67.39 67.12 61.35 59.03 48.56 31.46 5 29.60 14.2837.74 39.23 34.46 23.84 11.91 6 0.85 0.85 0.89 1.10 1.09 0.74 1.15 72.66 2.57 2.93 3.16 2.99 3.17 2.74 8 0.25 0.24 0.29 0.33 0.29 0.30 0.269 1.09 1.06 1.23 1.33 1.27 1.30 1.11 10 0.32 0.32 0.33 0.34 0.32 0.310.32 11 18.19 13.50 12.83 14.49 13.72 11.25 16.11 12 NA NA NA NA NA NANA 13 0.46 0.31 0.37 0.43 0.39 0.24 0.32 14 1.00 1.00 1.00 1.00 1.001.00 1.00 15 NA NA NA NA NA NA NA 16 104.00 89.20 97.80 113.60 109.20108.40 91.60 17 140.20 140.40 140.40 145.80 143.00 140.40 133.00 18105.78 112.21 103.83 105.74 107.45 106.34 78.29 19 3.42 2.67 3.64 3.653.51 3.17 2.50 20 36.20 51.20 42.60 32.20 33.80 32.00 41.40 21 12.301.10 8.50 18.67 11.00 7.40 1.50 22 4.69 5.47 5.92 6.16 6.88 5.17 7.72 236.85 8.51 8.32 9.80 9.28 7.81 11.96 24 0.81 0.75 0.83 0.74 0.88 0.790.75 25 0.91 0.85 0.96 0.82 0.94 0.91 0.92 26 0.44 0.42 0.41 0.41 0.440.47 0.48 27 77.13 85.00 67.53 50.87 55.67 64.67 50.93 28 76.60 35.3075.30 68.50 66.80 69.30 32.20 29 107.42 116.31 114.38 104.47 105.5992.02 58.82 30 0.037 0.044 0.047 0.042 0.056 0.056 0.033 31 8.24 9.148.71 9.82 10.00 8.36 12.49 Table 98. Provided are the values of each ofthe parameters measured in Barley Hordeum spontaneum accessions (30-34,36-37) according to the correlation identifications (see Table 88).

TABLE 99 Measured parameters of correlation Ids in Barley Hardeumspontaneum accessions Ecotype/ Treatment Line-38 Line-41 Line-42 Line-43Line-44 Line-45 Line-46 1 NA 21.20 14.49 17.05 12.52 9.87 10.75 2 31.0036.89 24.24 27.81 23.34 31.77 27.36 3 44.56 50.54 44.04 50.12 40.3755.92 33.55 4 59.31 91.79 63.37 69.41 58.51 61.56 42.29 5 NA 55.88 31.3432.88 35.99 42.56 19.47 6 1.32 1.40 0.93 0.96 0.82 1.34 1.16 7 2.69 2.673.05 2.77 2.94 3.18 3.06 8 0.26 0.28 0.30 0.26 0.29 0.33 0.30 9 1.101.09 1.28 1.14 1.25 1.32 1.25 10 0.33 0.36 0.33 0.33 0.32 0.35 0.34 1121.71 20.75 11.50 17.55 10.65 15.98 14.58 12 NA NA NA NA NA NA NA 130.66 0.75 0.31 0.47 0.25 0.53 0.43 14 1.00 1.40 1.00 1.00 1.00 1.00 1.0015 NA NA NA NA NA NA NA 16 115.60 116.80 111.00 111.00 111.00 111.0099.20 17 145.80 144.20 140.20 146.00 140.20 143.00 133.00 18 107.63126.08 107.15 106.69 96.26 99.81 91.75 19 NA NA 3.44 3.52 3.60 3.75 2.9420 30.20 27.40 29.25 35.00 29.20 32.00 33.80 21 NA 15.50 10.70 15.0011.70 6.90 5.50 22 8.37 8.38 5.09 5.03 4.88 8.33 7.43 23 11.32 10.128.27 8.45 7.95 10.21 11.52 24 0.86 0.99 0.72 0.65 0.72 0.96 0.76 75 0.941.06 0.82 0.76 0.82 1.04 0.91 26 0.43 0.36 0.41 0.42 0.41 0.48 0.44 2748.40 45.80 73.53 79.33 61.67 49.13 55.10 28 NA 75.13 71.20 86.70 90.7071.40 58.50 29 110.88 149.88 107.41 119.53 98.88 117.49 75.84 30 0.036NA 0.057 0.040 0.034 0.048 0.045 31 11.03 10.44 8.66 9.91 8.51 10.1811.82 Table 99. Provided are the values of each of the parametersmeasured in Barley Hordeum spontaneum accessions (38, 41-46) accordingto the correlation identifications (see Table 88).

TABLE 100 Measured parameters of correlation Ids in Barley Hordeumspontaneum accessions Ecotype/Treatment Line-47 Line-48 Line-49 Line-51Line-52 Line-53 1 10.80 14.99 16.12 NA 15.51 6.88 2 25.70 24.92 26.3124.82 26.46 21.49 3 31.74 50.70 44.59 26.20 57.49 47.76 4 41.24 71.3873.03 52.91 73.30 65.81 5 26.16 39.22 49.89 NA 38.72 29.92 6 1.18 0.941.05 1.01 0.84 0.75 7 2.75 2.62 2.99 3.24 2.90 2.65 8 0.26 0.24 0.290.32 0.26 0.25 9 1.13 1.06 1.25 1.36 1.17 1.10 10 0.32 0.32 0.33 0.330.32 0.31 11 17.44 18.90 14.60 13.07 19.84 17.16 12 NA NA NA NA NA NA 130.45 0.47 0.40 0.32 0.50 0.38 14 1.00 1.00 1.00 1.00 1.00 1.00 15 NA NANA NA NA NA 16 105.80 111.00 117.20 122.60 111.00 107.60 17 133.00143.00 148.20 152.00 142.40 140.40 18 80.80 105.57 101.87 80.26 105.0298.42 19 3.29 3.68 3.84 NA 3.66 3.41 20 27.20 32.00 31.00 29.40 31.4032.80 21 10.30 12.40 13.33 NA 18.30 6.60 22 6.71 6.61 7.10 8.62 7.165.75 23 10.17 9.09 9.79 11.73 10.01 8.78 24 0.77 0.94 0.85 0.87 0.860.77 25 0.92 0.97 0.95 0.97 0.94 0.89 26 0.46 0.42 0.38 0.33 0.44 0.4227 56.67 62.20 70.93 45.00 74.58 74.53 28 90.90 87.50 108.50 NA 113.5095.60 29 72.98 122.08 117.62 79.11 130.79 113.58 30 0.039 0.065 0.0460.040 0.040 0.045 31 10.58 9.42 10.04 11.67 10.60 9.72 Table 100.Provided are the values of each of the parameters measured in BarleyHordeum spontaneum accessions (47-49, 51-53) according to thecorrelation identifications (see Table 88).

TABLE 101 Measured parameters of correlation Ids in Barley Hordeumvulgare accessions Ecotype/ Treatment Line-1 Line-2 Line-4 Line-5 Line-6Line-8 Line-9 1 4.31 18.25 40.15 33.22 NA 16.67 5.64 1 50.11 49.98 52.4347.22 49.33 61.33 49.97 3 80.88 60.47 69.45 61.03 63.22 91.89 99.05 446.33 85.03 127.37 79.51 82.95 82.88 56.80 5 11.34 52.57 126.89 60.56 NA44.57 9.71 6 3.33 1.56 3.11 3.18 2.85 4.13 3.47 7 2.62 2.41 2.67 2.622.59 2.78 2.66 8 0.30 0.28 0.30 0.29 0.29 0.33 0.29 9 1.09 0.97 1.071.09 1.07 1.15 1.09 10 0.40 0.41 0.41 0.39 0.39 0.42 0.39 11 56.51 21.0544.35 47.12 43.51 58.33 56.03 12 64.98 37.47 51.69 49.15 46.44 78.1879.86 13 2.91 1.02 2.33 2.23 2.14 3.47 2.60 14 4.20 1.00 2.60 2.60 1.001.00 5.00 15 0.51 0.25 0.26 0.35 0.32 0.45 0.51 16 90.80 124.40 NA122.00 NA 111.60 86.80 17 148.00 170.00 170.00 167.40 170.00 156.20159.60 18 83.97 79.87 122.46 108.03 87.00 104.01 70.78 19 2.45 3.96 4.754.12 NA 3.82 2.30 20 57.20 45.60 NA 48.00 NA 44.60 72.80 21 1.00 9.2019.20 14.63 NA 6.30 1.20 22 9.90 7.82 11.07 10.17 9.98 9.89 9.58 23 9.4910.26 7.97 8.42 8.12 6.39 7.73 24 1.23 0.87 1.68 1.47 1.51 1.83 1.50 251.41 1.05 1.79 1.60 1.61 1.93 1.59 26 0.64 0.42 0.35 0.44 0.43 0.51 0.6427 45.27 56.27 32.42 35.40 36.73 32.10 48.53 28 24.00 48.70 47.60 45.00NA 38.50 21.50 29 127.22 145.50 196.82 140.54 146.17 178.63 155.85 300.053 0.059 0.051 0.053 0.047 0.052 0.062 31 9.57 NA 7.93 8.13 NA 5.657.94 Table 101. Provided are the values of each of the parametersmeasured in Barley Hordeum vulgare accessions (1-2, 4-6, 8-9) accordingto the correlation identifications (see Table 88).

TABLE 102 Measured parameters of correlation Ids in Barley Hordeumvulgare accessions Ecotype/ Treatment Line-10 Line-11 Line-12 Line-13Line-14 Line-15 Line-16 1 5.29 18.34 4.03 8.83 4.82 29.49 5.01 2 51.7056.46 53.97 50.36 56.81 57.98 51.44 3 66.99 60.22 87.61 71.76 76.7181.14 77.90 4 64.14 54.23 73.23 49.50 47.63 66.51 77.50 5 38.18 46.7442.32 11.62 9.33 47.56 30.93 6 3.15 1.88 3.35 3.60 3.24 3.12 1.69 7 2.632.28 2.54 2.37 2.71 2.90 2.28 8 0.30 0.28 0.30 0.27 0.32 0.34 0.27 91.08 0.88 1.03 0.96 1.12 1.22 0.89 10 0.39 0.45 0.42 0.41 0.41 0.41 0.4211 59.08 27.27 55.87 61.53 50.80 45.48 24.77 12 54.34 46.37 71.89 56.2461.63 64.82 56.43 13 2.84 1.51 2.84 2.98 2.85 2.39 1.21 14 3.00 1.001.00 2.20 3.00 1.00 1.00 15 0.41 0.40 0.45 0.48 0.50 0.44 0.36 16 106.20117.80 111.60 85.40 90.00 113.20 113.40 17 157.00 162.20 159.60 157.00150.50 158.00 170.00 18 98.11 57.88 94.52 73.20 78.65 90.73 64.27 193.60 3.83 3.63 2.43 2.26 3.89 3.46 20 50.80 44.40 46.00 71.60 61.5044.80 56.60 21 2.10 10.00 2.60 1.63 1.00 17.00 3.00 22 11.19 8.76 10.4910.83 11.23 7.89 9.15 23 8.45 10.55 7.60 7.87 9.42 6.68 12.05 24 1.570.96 1.63 1.63 1.43 1.45 0.88 25 1.71 1.17 1.75 1.72 1.58 1.52 1.03 260.51 0.53 0.55 0.61 0.62 0.55 0.50 27 29.80 50.80 32.40 26.80 42.4239.73 71.33 28 36.10 57.25 42.20 19.13 21.63 59.80 62.50 29 131.13114.45 160.84 121.26 124.34 147.66 155.41 30 0.051 0.062 0.060 0.0560.045 0.051 0.037 31 8.55 10.59 7.44 7.36 9.60 6.23 NA Table 102.Provided are the values of each of the parameters measured in BarleyHordeum vulgare accessions (10-16) according to the correlationidentifications (see Table 88).

TABLE 103 Measured parameters of correlation Ids in Barley Hordeumvulgare accessions Ecotype/ Treatment Line-17 Line-18 Line-19 Line-54Line-55 1 3.74 11.42 5.13 7.07 6.72 2 58.07 53.45 48.66 43.66 47.91 368.17 70.73 54.13 43.70 68.61 4 81.58 67.92 81.05 56.28 NA 5 NA 35.4938.41 14.62 67.47 6 1.66 3.50 1.16 3.71 2.78 7 2.42 2.65 2.16 2.24 2.568 0.30 0.30 0.26 0.25 0.28 9 0.96 1.08 0.83 0.88 1.05 10 0.44 0.40 0.420.40 0.38 11 21.15 59.72 17.46 65.39 43.77 12 49.68 54.97 40.33 34.5853.97 13 1.18 2.93 0.83 2.64 2.06 14 3.80 3.80 1.00 5.00 1.80 15 0.330.40 0.29 0.35 NA 16 98.50 109.60 119.40 88.40 128.00 17 170.00 155.20170.00 157.00 170.00 18 82.73 94.12 63.47 93.79 90.30 19 NA 3.60 3.642.18 4.23 20 71.50 45.60 50.60 68.60 42.00 21 1.00 3.80 4.20 2.50 3.1022 8.57 11.30 7.04 10.74 10.04 23 10.74 8.60 8.94 8.54 8.59 24 0.92 1.560.92 1.49 1.45 25 1.10 1.72 1.08 1.68 1.57 26 0.45 0.51 0.39 0.44 NA 2765.40 33.27 82.47 20.80 38.00 28 31.20 34.00 78.90 15.60 43.20 29 149.76138.64 135.18 99.98 NA 30 0.047 0.043 0.056 0.053 0.054 31 NA 8.57 NA8.26 9.22 Table 103. Provided are the values of each of the parametersmeasured in Barley Hordeum vulgare accessions (17-19, 54-55) accordingto the correlation identifications see Table 88).

TABLE 104 Correlation between the expression level of the selectedpolynucleotides of the invention and their homologues in specifictissues or developmental stages and the phenotypic perfomance across all55 Barley accessions Gene Exp. Corr. Name R P value set Set ID LYM10150.71 9.98E−04 2 12 LYM1016 0.71 9.48E−04 3 12 LYM1026 0.71 1.08E−08 3 2LYM1026 0.72 6.15E−09 3 6 LYM1026 0.74 1.45E−09 2 10 LYM1027 0.891.52E−06 2 15 LYM1031 0.71 1.27E−03 2 15 LYM1031 0.73 5.43E−09 2 26LYM1031 0.74 1.54E−09 2 11 LYM1034 0.75 5.68E−04 3 15 LYM1034 0.768.23E−11 1 26 LYM1040 0.76 3.92E−04 3 15 LYM1042 0.74 1.75E−09 3 2LYM1042 0.77 7.63E−11 2 2 LYM1048 0.70 1.94E−08 3 2 LYM1048 0.724.07E-09 3 3 LYM1048 0.73 1.18E−09 1 24 LYM1048 0.75 1.12E−10 1 13LYM1048 0.74 3.47E−10 1 25 LYM1048 0.75 5.33E−10 2 2 LYM1048 0.731.84E−09 2 3 LYM1048 0.72 4.11E−09 2 10 LYM1048 0.75 2.50E−10 4 13LYM1048 0.73 1.23E−09 4 26 LYM1051 0.71 8.06E−09 3 3 LYM1053 0.733.50E−09 3 26 LYM1054 0.73 3.53E−09 2 26 LYM1056 0.72 2.60E−09 1 6LYM1056 0.71 4.18E−09 1 11 LYM1056 0.73 3.24E−09 2 6 LYM1060 0.701.66E−03 3 15 LYM1066 0.71 1.30E−08 3 2 LYM1066 0.73 6.30E−10 1 24LYM1066 0.80 8.16E−13 1 13 LYM1066 0.76 5.19E−11 1 10 LYM1066 0.752.27E−10 1 11 LYM1066 0.77 1.47E−10 2 13 LYM1066 0.74 1.23E−09 2 10LYM1066 0.78 1.72E−11 4 2 LYM1069 0.73 8.31E−04 2 15 LYM1070 0.711.54E−03 2 15 LYM1015 0.83 4.34E−05 2 15 LYM1023 0.77 2.88E−04 3 15LYM1026 0.74 1.61E−09 3 13 LYM1026 0.72 6.13E−09 2 2 LYM1027 0.822.64E−05 2 12 LYM1031 0.78 4.17E−11 2 13 LYM1031 0.76 2.16E−10 2 3LYM1031 0.76 3.24E−10 2 6 LYM1034 0.70 1.15E−03 3 12 LYM1034 0.801.31E−04 1 15 LYM1037 0.74 7.64E−04 2 15 LYM1041 0.71 9.67E−04 3 12LYM1042 0.74 9.90E−10 3 3 LYM1042 0.76 2.06E−10 2 10 LYM1048 0.741.37E−09 3 13 LYM1048 0.73 2.10E−09 3 6 LYM1048 0.72 1.23E−09 1 2LYM1048 0.76 7.08E−11 1 6 LYM1048 0.73 1.13E−09 1 11 LYM1048 0.755.04E−10 2 13 LYM1048 0.74 1.05E−09 2 6 LYM1048 0.72 2.45E−09 4 2LYM1048 0.72 2.53E−09 4 3 LYM1048 0.72 1.85E−09 4 6 LYM1053 0.701.74E−03 3 15 LYM1054 0.77 3.41E−04 2 15 LYM1056 0.72 1.61E−09 1 24LYM1056 0.72 1.93E−09 1 25 LYM1056 0.71 9.36E−09 2 13 LYM1056 0.711.10E−08 2 11 LYM1062 0.71 1.45E−03 1 15 LYM1066 0.71 1.01E−08 3 13LYM1066 0.80 1.00E−12 1 2 LYM1066 0.78 7.36E−12 1 6 LYM1066 0.768.58E−11 1 25 LYM1066 0.75 8.34E−10 2 2 LYM1066 0.74 1.63E−09 2 6LYM1066 0.72 4.02E−09 2 11 LYM1066 0.79 7.32E−12 4 10 LYM1070 0.701.70E−03 1 15 LYM1076 0.79 1.49E−04 4 15 Table 104. Provided are thecorrelations (R) and p-values (P) between the expression levels ofselected genes of some embodiments of the invention in various tissuesor developmental stages (Expression sets) and the phenotypic performancein various yield (seed yield, oil yield, oil content), biomass, growthrate and/or vigor components [Correlation (Corr.) vector (Vec.)Expression (Exp.)] Corr. Vector = correlation vector specified in Tableb; Exp. Set = expression set specified in Table 87.

TABLE 105 Correlation between the expression level of the selectedpolynucleotides of the invention and their homologues in specifictissues or developmental stages and the phenotypic performance across 27Barley Hordeum spontaneum accessions Gene Exp. Corr Name R P value setSet ID LYM1010 0.76 2.54E−05 2 13 LYM1018 0.72 8.37E−05 3 11 LYM10570.75 1.02E−05 1 8 LYM1059 0.74 4.89E−05 2 17 LYM1073 0.72 1.22E−04 2 24LYM1018 0.80 2.95E−06 3 13 LYM1052 0.75 3.28E−05 2 26 LYM1059 0.711.47E−04 2 16 LYM1061 0.87 1.66E−06 2 5 Table 105 provided are thecorrelations (R) and p-values (P) between the expression levels ofselected genes of some embodiments of the invention in various tissuesor developmental stages (Expression sets) and the phenotypic performancein various yield (seed yield, oil yield, oil content), biomass, growthrate and/or vigor components [Correlation (Corr.) vector (Vec.)Expression (Exp.)] Corr. Vector = correlation vector specified in Tableb; Exp. Set = expression set specified in Table 87.

TABLE 106 Correlation between the expression level of the selectedpolynucleotides of the invention and their homologues in specifictissues or developmental stages and the phenotypic performance across 19Barley Hordeum vulgare accessions Gene Exp. Corr. Name R P value set SetID LYM1015 0.71 9.98E−04 2 12 LYM1015 0.80 1.16E−04 2 26 LYM1022 0.762.33E−04 1 2 LYM1023 0.79 1.45E−04 3 26 LYM1024 0.73 1.25E−03 1 20LYM1027 0.80 7.37E−05 2 13 LYM1027 0.89 1.52E−06 2 15 LYM1027 0.825.61E−05 2 26 LYM1027 0.71 1.07E−03 2 11 LYM1031 0.71 1.27E−03 2 15LYM1033 0.77 2.69E−04 2 26 LYM1034 0.75 5.68E−04 3 15 LYM1034 0.801.31E−04 1 15 LYM1035 0.70 7.39E−03 4 31 LYM1037 0.80 1.22E−04 2 26LYM1040 0.76 3.92E−04 3 15 LYM1041 0.71 9.67E−04 3 12 LYM1041 0.721.19E−03 3 26 LYM1044 0.70 1.63E−03 2 26 LYM1048 0.74 7.03E−04 4 26LYM1052 0.74 3.94E−04 3 24 LYM1052 0.83 1.73E−05 3 13 LYM1052 0.76273E−04 3 25 LYM1052 0.73 6.32E−04 4 22 LYM1053 0.70 1.74E−03 3 15LYM1054 0.74 4.34E−04 2 9 LYM1054 0.71 8.61E−04 2 7 LYM1055 0.755.19E−04 2 26 LYM1056 0.82 3.16E−05 3 13 LYM1056 0.84 1.25E−05 3 25LYM1056 0.85 7.66E−06 1 24 LYM1056 0.85 7.13E−06 1 13 LYM1056 0.864.21E−06 1 25 LYM1056 0.86 4.81E−06 2 13 LYM1056 0.70 1.17E−03 2 25LYM1058 0.72 1.18E−03 2 26 LYM1060 0.70 1.66E−03 3 15 LYM1061 0.701.75E−03 4 26 LYM1062 0.71 1.45E−03 1 15 LYM1062 0.73 5.21E−04 1 11LYM1063 0.74 6.54E−04 2 26 LYM1064 0.71 9.49E−04 2 24 LYM1065 0.719.04E−04 3 2 LYM1066 0.75 4.82E−04 2 26 LYM1068 0.75 3.48E−04 4 2LYM1069 0.73 8.31E−04 2 15 LYM1070 0.70 1.70E−03 1 15 LYM1070 0.755.84E−04 4 26 LYM1073 0.74 9.89E−04 1 5 LYM1076 0.79 1.49E−04 4 15LYM1234 0.74 7.27E−04 4 26 LYM1015 0.83 4.34E−05 2 15 LYM1016 0.719.48E−04 3 12 LYM1023 0.77 2.88E−04 3 15 LYM1023 0.77 3.31E−04 1 26LYM1025 0.79 1.47E−04 1 4 LYM1027 0.82 2.64E−05 2 12 LYM1027 0.744.23E−04 2 3 LYM1027 0.73 6.33E−04 2 6 LYM1030 0.73 8.34E−04 1 26LYM1031 0.77 3.24E−04 2 26 LYM1034 0.70 1.15E−03 3 12 LYM1034 0.721.03E−03 3 26 LYM1034 0.78 1.90E−04 1 26 LYM1037 0.74 7.64E−04 2 15LYM1038 0.71 1.39E−03 2 26 LYM1040 0.76 4.04E−04 3 26 LYM1041 0.744.67E−04 3 3 LYM1043 0.74 7.53E−04 4 26 LYM1046 0.70 2.50E−03 2 20LYM1048 0.74 6.03E−04 2 26 LYM1052 0.84 1.38E−05 3 22 LYM1052 0.807.25E−05 3 6 LYM1052 0.79 8.36E−05 3 11 LYM1052 0.70 1.22E−03 2 22LYM1053 0.77 3.23E−04 3 26 LYM1054 0.77 3.41E−04 2 15 LYM1054 0.755.84E−04 2 26 LYM1056 0.84 1.53E−05 3 24 LYM1056 0.84 1.27E−05 3 6LYM1056 0.77 2.08E−04 3 11 LYM1056 0.71 9.05E−04 1 22 LYM1056 0.864.42E−06 1 6 LYM1056 0.89 1.02E−06 1 11 LYM1056 0.83 2.31E−05 2 6LYM1056 0.85 7.76E−06 2 11 LYM1059 0.76 4.14E−04 4 28 LYM1060 0.755.28E−04 3 26 LYM1062 0.73 6.58E−04 1 13 LYM1062 0.76 2.44E−04 1 6LYM1063 0.77 1.99E−04 3 14 LYM1064 0.71 1.04E−03 3 14 LYM1064 0.728.21E−04 2 25 LYM1065 0.79 9.97E−05 1 27 LYM1069 0.71 1.48E−03 4 26LYM1069 0.81 6.87E−05 2 26 LYM1070 0.77 2.65E−04 1 26 LYM1070 0.711.54E−03 2 15 LYM1076 0.70 1.15E−03 1 2 LYM1076 0.82 4.74E−05 4 26 Table106. Provided are the correlations (R) and p-values (P) between theexpression levels of selected genes of some embodiments of the inventionin various tissues or developmental stages (Expression sets) and thephenotypic performance in various yield (seed yield, oil yield, oilcontent), biomass, growth rate and/or vigor components [Correlation(Con) vector (Vec.) Expression (Exp.)] COM Vector = correlation vectorspecified in Table b; Exp. Set = expression set specified in Table 87.

Example 15 Gene Cloning and Generation of Binary Vectors for PlantExpression

To validate their role in improving yield, selected genes wereover-expressed in plants, as follows.

Cloning Strategy

Selected genes from those presented in Examples 1-14 hereinabove werecloned into binary vectors for the generation of transgenic plants. Forcloning, the full-length open reading frames (ORFs) were identified. ESTclusters and in some cases mRNA sequences were analyzed to identify theentire open reading frame by comparing the results of severaltranslation algorithms to known proteins from other plant species.

In order to clone the full-length cDNAs, reverse transcription (RT)followed by polymerase chain reaction (PCR; RT-PCR) was performed ontotal RNA extracted from leaves, roots or other plant tissues, growingunder normal/limiting or stress conditions. Total RNA extraction,production of cDNA and PCR amplification was performed using standardprotocols described elsewhere (Sambrook J., E. F. Fritsch, and T.Maniatis. 1989. Molecular Cloning. A Laboratory Manual, 2nd Ed. ColdSpring Harbor Laboratory Press. New York) which are well known to thoseskilled in the art. PCR products were purified using PCR purificationkit (Qiagen).

Usually, 2 sets of primers were prepared for the amplification of eachgene, via nested PCR (if required). Both sets of primers were used foramplification on a cDNA. In case no product was obtained, a nested PCRreaction was performed. Nested PCR was performed by amplification of thegene using external primers and then using the produced PCR product as atemplate for a second PCR reaction, where the internal set of primerswere used. Alternatively, one or two of the internal primers were usedfor gene amplification, both in the first and the second PCR reactions(meaning only 2-3 primers are designed for a gene). To facilitatefurther cloning of the cDNAs, an 8-12 base pairs (bp) extension wasadded to the 5′ of each internal primer. The primer extension includesan endonuclease restriction site. The restriction sites were selectedusing two parameters: (a) the restriction site does not exist in thecDNA sequence; and (b) the restriction sites in the forward and reverseprimers were designed such that the digested cDNA was inserted in thesense direction into the binary vector utilized for transformation.

PCR products were digested with the restriction endonucleases (NewEngland BioLabs Inc) according to the sites designed in the primers.Each digested/undigested PCR product was inserted into a high copyvector pUC19 (New England BioLabs Inc], or into plasmids originatingfrom this vector. In some cases the undigested PCR product was insertedinto pCR-Blunt II-TOPO (Invitrogen) or into pJET1.2 (CloneJET PCRCloning Kit, Thermo Scientific) or directly into the binary vector. Thedigested/undigested products and the linearized plasmid vector wereligated using T4 DNA ligase enzyme (Roche. Switzerland or othermanufacturers). In cases where pCR-Blunt II-TOPO is used no T4 ligase isneeded.

Sequencing of the inserted genes was performed, using the ABI 377sequencer (Applied Biosystems). In some cases, after confirming thesequences of the cloned genes, the cloned cDNA was introduced into amodified pGI binary vector containing the At6669 promoter (e.g., pQFNc)and the NOS terminator (SEQ ID NO: 8201) via digestion with appropriaterestriction endonucleases.

In case of Brachypodium transformation, after confirming the sequencesof the cloned genes, the cloned cDNAs were introduced into pEBbVNi (FIG.9A) containing 35S promoter (SEQ ID NO: 8202) and the NOS terminator(SEQ ID NO:8201) via digestion with appropriate restrictionendonucleases. The genes were cloned downstream to the 35S promoter andupstream to the NOS terminator.

Several DNA sequences of the selected genes were synthesized by GeneArt(Life Technologies, Grand Island, N.Y., USA). Synthetic DNA was designedin silico. Suitable restriction enzymes sites were added to the clonedsequences at the 5′ end and at the 3′ end to enable later cloning intothe desired binary vector.

Binary vectors—The pPI plasmid vector was constructed by inserting asynthetic poly-(A) signal sequence, originating from pGL3 basic plasmidvector (Promega, GenBank Accession No. U47295; nucleotides 4658-4811)into the HindIII restriction site of the binary vector pBI101.3(Clontech, GenBank Accession No. U12640), pGI is similar to pPI, but theoriginal gene in the backbone is GUS-Intron and not GUS.

The modified pGI vector (e.g., pQFN, pQFNc, pQYN_6669, pQNa_RP, pQFYN orpQXNc) is a modified version of the pGI vector in which the cassette isinverted between the left and right borders so the gene and itscorresponding promoter are close to the right border and the NPTII geneis close to the left border.

At6669, the new Arabidopsis thaliana promoter sequence (SEQ ID NO:8190)was inserted in the modified pGI binary vector, upstream to the clonedgenes, followed by DNA ligation and binary plasmid extraction frompositive E. coli colonies, as described above. Colonies were analyzed byPCR using the primers covering the insert which were designed to spanthe introduced promoter and gene. Positive plasmids were identified,isolated and sequenced.

pEBbVNi (FIG. 9A) is a modified version of pJJ2LB in which theHygromycin resistance gene was replaced with the BAR gene which confersresistance to the BASTA herbicide [BAR gene coding sequence is providedin GenBank Accession No. JQ293091.1 (SEQ ID NO:8203); furtherdescription is provided in Akama K. et al. “EfficientAgrobacterium-mediated transformation of Arabidopsis thaliana using thebar gene as selectable marker”, Plant Cell Rep. 1995, 14(7):450-4;Christiansen P, et al. “A rapid and efficient transformation protocolfor the grass Brachypodium distachyon”, Plant Cell Rep. 2005 March;23(10-11):751-8. Epub 2004 Oct. 19; and Picurar D I, et al. “Ahigh-throughput Agrobacterium-mediated transformation system for thegrass model species Brachypodium distachyon L”. Transgenic Res. 200817(5):965-75: each of which is fully incorporated herein by reference inits entirety]. The pEBbVNi construct contains the 35S promoter (SEQ IDNO:8202). pJJ2LB is a modified version of pCambia0305.2 (Cambia).

In case genomic DNA was cloned, the genes were amplified by direct PCRon genomic DNA extracted from leaf tissue using the DNAeasy kit (QiagenCat. No. 69104).

Selected genes cloned by the present inventors are provided in Table 107below.

TABLE 107 Cloning Table Polypeptide Gene High copy Primers usedPolynucleotide SEQ ID Name plasmid Organism SEQ ID NOs: SEQ ID NO: NO:LYM1010 LYM1010_GA 269 474 LYM1011 LYM1011_GA 270 475 LYM1012 LYM1012_GA271 476 LYM1013 LYM1013 BARLEY Hordeum vulgare L. 8204, 8352, 8500, 8576272 715 LYM1014 LYM1014_GA 773 478 LYM1015 LYM1015 BARLEY Hordeumvulgare L. 8205, 8353, 8205, 8353 274 479 LYM1016 LYM1016 BARLEY Hordeumvulgare L. 8206, 8354, 8501, 8577 275 480 LYM1017 LYM1017_GA 276 481LYM1018 LYM1018 BARLEY Hordeum vulgare L. 8207, 8355, 8207, 8355 277 716LYM1019 LYM1019 BARLEY Hordeum vulgare L. 8208, 8356, 8208, 8356 278 483LYM1020 LYM1020 BARLEY Hordeum vulgare L. 8209, 8357, 8502, 8578 279 717LYM1021 LYM1021 BARLEY Hordeum vulgare L. 8210, 8358, 8210, 8358 280 485LYM1022 LYM1022 BARLEY Hordeum vulgare L. 8211, 8359, 8211, 8359 281 718LYM1023 LYM1023 BARLEY Hordeum vulgare L. 8212, 8360, 8212, 8360 282 719LYM1024 LYM1024 BARLEY Hordeum vulgare L. 8213, 8361, 8213, 8361 283 488LYM1025 LYM1025 BARLEY Hordeum vulgare L. 8214, 8362, 8503, 8579 284 489LYM1026 LYM1026 BARLEY Hordeum vulgare L. 8215, 8363, 8504, 8580 285 490LYM1027 LYM1027 BARLEY Hordeum vulgare L. 8216, 8364, 8216, 8364 286 491LYM1028 LYM1028_GA 287 492 LYM1029 LYM1029 BARLEY Hordeum vulgare L.8217, 8365, 8505, 8581 288 493 LYM1030 LYM1030 BARLEY Hordeum vulgare L.8218, 8366, 8218, 8366 289 720 LYM1033 LYM1033 BARLEY Hordeum vulgare L.8220, 8368, 8220, 8368 290 497 LYM1034 LYM1034 BARLEY Hordeum vulgare L.8221, 8369, 8506, 8582 291 721 LYM1035 LYM1035_GA 292 499 LYM1036LYM1036_GA 293 500 LYM1037 LYM1037_GA 294 501 LYM1038 LYM1038 BARLEYHordeum vulgare L. 8222, 8370, 8507, 8583 295 502 LYM1040 LYM1040 BARLEYHordeum vulgare L. 8223, 8371, 8508, 8584 296 503 LYM1041 LYM1041_GA 297504 LYM1042 LYM1042_GA 298 505 LYM1043 LYM1043 BARLEY Hordeum vulgare L.8224, 8372, 8224, 8372 299 506 LYM1044 LYM1044_GA 300 507 LYM1046LYM1046 BARLEY Hordeum vulgare L. 8225, 8373, 8509, 8585 301 508 LYM1047LYM1047 BARLEY Hordeum vulgare L. 8226, 8374, 8226, 8374 302 509 LYM1049LYM1049 BARLEY Hordeum vulgare L. 8227, 8375, 8227, 8375 303 511 LYM1051LYM1051 BARLEY Hordeum vulgare L. 8228, 8376, 8510, 8586 304 722 LYM1052LYM1052 BARLEY Hordeum vulgare L. 8229, 8377, 8229, 8377 305 723 LYM1053LYM1053 BARLEY Hordeum vulgare L. 8230, 8378, 8511, 8587 306 724 LYM1054LYM1054 BARLEY Hordeum vulgare L. 8231, 8379, 8512, 8588 307 515 LYM1055LYM1055_GA 308 516 LYM1056 LYM1056 BARLEY Hordeum vulgare L. 8232, 8380,8232, 8380 309 517 LYM1057 LYM1057 BARLEY Hordeum vulgare L. 8233, 8381,8233, 8381 310 518 LYM1060 LYM1060 BARLEY Hordeum vulgare L. 8234, 8382,8513, 8589 311 521 LYM1061 LYM1061 BARLEY Hordeum vulgare L. 8235, 8383,8514, 8590 312 522 LYM1062 LYM1062 BARLEY Hordeum vulgare L. 8236, 8384,8236, 8384 313 523 LYM1063 LYM1063_GA 314 524 LYM1065 LYM1065 BARLEYHordeum vulgare L. 8237, 8385, 8515, 8591 315 526 LYM1066 LYM1066 BARLEYHordeum vulgare L. 8238, 8386, 8516, 8592 316 527 LYM1068 LYM1068_GA 317528 LYM1069 LYM1069_GA 318 529 LYM1070 LYM1070 BARLEY Hordeum vulgare L.8239, 8387, 8239, 8387 319 725 LYM1071 LYM1071 BARLEY Hordeum vulgare L.8240, 8388, 8240, 8388 320 726 LYM1072 LYM1072 BARLEY Hordeum vulgare L.8241, 8389, 8241, 8389 321 532 LYM1073 LYM1073 BARLEY Hordeum vulgare L.322 533 LYM1074 LYM1074 BARLEY Hordeum vulgare L. 8243, 8391, 8243, 8391323 534 LYM1075 LYM1075 BARLEY Hordeum vulgare L. 8244, 8392, 8517, 8593324 727 LYM1078 LYM1078 BRACHYPODIUM 8246, 8394, 8246, 8394 325 728Brachypodiums dis LYM1079 LYM1079 BRACHYPODIUM 8247, 8395, 8519, 8595326 538 Brachypodiums dis LYM1082 LYM1082 BRACHYPODIUM 8248, 8396, 8248,8396 327 539 Brachypodiums dis LYM1084 LYM1084 BRACHYPODIUM 8249, 8397,8520, 8596 328 540 Brachypodiums dis LYM1085 LYM1085 BRACHYPODIUM 8250,8398, 8521, 8597 329 729 Brachypodiums dis LYM1086 LYM1086 BRACHYPODIUM8251, 8399, 8522, 8598 330 730 Brachypodiums dis LYM1087 LYM1087BRACHYPODIUM 8252, 8400, 8252, 8400 331 543 Brachypodiums dis LYM1088LYM1088_GA 332 544 LYM1089 LYM1089_GA 333 545 LYM1090 LYM1090BRACHYPODIUM 8253, 8401, 8523, 8599 334 731 Brachypodiums dis LYM1092LYM1092 BRACHYPODIUM 8254, 8402, 8254, 8600 335 548 Brachypodiums disLYM1093 LYM1093 BRACHYPODIUM 8255, 8403, 8524, 8601 336 549Brachypodiums dis LYM1094 LYM1094 BRACHYPODIUM 8256, 8404, 8525, 8602337 732 Brachypodiums dis LYM1095 LYM1095_GA 338 551 LYM1096 LYM1096_GA339 552 LYM1097 LYM1097_GA 340 553 LYM1099 LYM1099 BRACHYPODIUM 8257,8405, 8526, 8603 341 555 Brachypodiums dis LYM1100 LYM1100 FOXTAILSetaria italica 8258, 8406, 8527, 8604 342 556 LYM1102 LYM1102 FOXTAILSetaria italica 8259, 8407, 8528, 8605 343 558 LYM1103 LYM1103 FOXTAILSetaria italica 8260, 8408, 8260, 8408 344 559 LYM1104 LYM1104_GA 345560 LYM1106 LYM1106_GA 346 562 LYM1107 LYM1107 FOXTAIL Setaria italica8261, 8409, 8261, 8409 347 563 LYM1108 LYM1108 FOXTAIL Setaria italica8262, 8410, 8529, 8606 348 564 LYM1109 LYM1109 FOXTAIL Setaria italica349 565 LYM1110 LYM1110 FOXTAIL Setaria italica 350 566 LYM1111 LYM1111FOXTAIL Setaria italica 8265, 8413, 8265, 8413 351 733 LYM1112LYM1112_GA 352 568 LYM1113 LYM1113 FOXTAIL Setaria italica 8266, 8414,8530, 8607 353 569 LYM1114 LYM1114 FOXTAIL Setaria italica 8267, 8415,8267, 8415 354 570 LYM1115 LYM1115 FOXTAIL Setaria italica 8268, 8416,8268, 8608 355 571 LYM1116 LYM1116 FOXTAIL Setaria italica 8269, 8417,8531, 8609 356 572 LYM1117 LYM1117 FOXTAIL Setaria italica 8270, 8418,8532, 8610 357 573 LYM1118 LYM1118 FOXTAIL Setaria italica 8271, 8419,8271, 8419 358 574 LYM1120 LYM1120_GA 359 576 LYM1121 LYM1121 FOXTAILSetaria italica 8272, 8420, 8533, 8611 360 577 LYM1122 LYM1122 FOXTAILSetaria italica 8273, 8421, 8534, 8612 361 578 LYM1123 LYM1123 FOXTAILSetaria italica 8274, 8422, 8535, 8613 362 734 LYM1124 LYM1124 FOXTAILSetaria italica 8275, 8423, 8536, 8614 363 580 LYM1125 LYM1125 FOXTAILSetaria italica 8276, 8424, 8276, 8424 364 735 LYM1126 LYM1126 FOXTAILSetaria italica 8277, 8425, 8277, 8425 365 582 LYM1127 LYM1127 FOXTAILSetaria italica 8278, 8426, 8537, 8615 366 736 LYM1128 LYM1128_GA 367584 LYM1129 LYM1129 MAIZE Zea mays L. 8279, 8427, 8279, 8427 368 585LYM1130 LYM1130 MAIZE Zea mays L. 8280, 8428, 8538, 8616 369 737 LYM1131LYM1131 MAIZE Zea mays L. 8281, 8429, 8539, 8617 370 738 LYM1132LYM1132_GA 371 588 LYM1133 LYM1133_GA 372 589 LYM1134 LYM1134_GA 373 590LYM1136 LYM1136_GA 374 591 LYM1137 LYM1137 MAIZE Zea mays L. 8282, 8430,8282, 8430 375 592 LYM1138 LYM1138 MAIZE Zea mays L. 8283, 8431, 8540,8618 376 593 LYM1139 LYM1139 MAIZE Zea mays L. 377 594 LYM1140LYM1140_GA 378 595 LYM1141 LYM1141 MAIZE Zea mays L. 8285, 8433, 8541,8619 379 596 LYM1142 LYM1142 MAIZE Zea mays L. 8286, 8434, 8286, 8434380 739 LYM1143 LYM1143 MAIZE Zea mays L. 8287, 8435, 8287, 8435 381 740LYM1146 LYM1146 MAIZE Zea mays L. 8288, 8436, 8542, 8620 382 599 LYM1149LYM1149 MAIZE Zea mays L. 8289, 8437, 8543, 8621 383 600 LYM1151 LYM1151MAIZE Zea mays L. 8290, 8438, 8290, 8438 384 601 LYM1152 LYM1152_GA 385602 LYM1153 LYM1153_GA 386 603 LYM1154 LYM1154 MAIZE Zea mays L. 8291,8439, 8544, 8622 387 604 LYM1155 LYM1155 MAIZE Zea mays L. 8292, 8440,8292, 8440 388 605 LYM1156 LYM1156 MAIZE Zea mays L. 8293, 8441, 8545,8623 389 606 LYM1157 LYM1157_GA 390 607 LYM1158 LYM1158 MAIZE Zea maysL. 8294, 8442, 8546, 8624 391 741 LYM1159 LYM1159 MAIZE Zea mays L.8295, 8443, 8547, 8625 392 609 LYM1160 LYM1160 MAIZE Zea mays L. 8296,8444, 8296, 8444 393 610 LYM1161 LYM1161 MAIZE Zea mays L. 8297, 8445,8548, 8626 394 611 LYM1163 LYM1163_GA 395 613 LYM1165 LYM1165 MAIZE Zeamays L. 8299, 8447, 8299, 8447 396 615 LYM1167 LYM1167 MAIZE Zea mays L.8300, 8448, 8549, 8627 397 742 LYM1168 LYM1168 MAIZE Zea mays L. 8301,8449, 8301, 8449 398 743 LYM1169 LYM1169 MAIZE Zea mays L. 8302, 8450,8550, 8628 399 618 LYM1170 LYM1170 MAIZE Zea mays L. 8303, 8451, 8551,8629 400 619 LYM1171 LXM1171 MAIZE Zea mays L. 8304, 8452, 8552, 8630401 744 LYM1172 LYM1172_GA 402 621 LYM1173 LYM1173 MAIZE Zea mays L.8305, 8453, 8305, 8453 403 745 LYM1174 LYM1174 MAIZE Zea mays L. 8306,8454, 8306, 8454 404 623 LYM1175 LYM1175_GA 405 624 LYM1176 LYM1176MAIZE Zea mays L. 8307, 8455, 8307, 8455 406 746 LYM1177 LYM1177 MAIZEZea mays L. 8308, 8456, 8308, 8456 407 747 LYM1178 LYM1178 MAIZE Zeamays L. 8309, 8457, 8309, 8457 408 627 LYM1179 LYM1179 MAIZE Zea mays L.8310, 8458, 8553, 8631 409 628 LYM1180 LXM1180 MAIZE Zea mays L. 8311,8459, 8311, 8459 410 629 LYM1181 LYM1181 MAIZE Zea mays L. 8312, 8460,8554, 8632 411 630 LYM1182 LYM1182_GA 412 631 LYM1183 LYM1183 MAIZE Zeamays L. 8313, 8461, 8313, 8461 413 632 LYM1184 LYM1184 MAIZE Zea mays L.8314, 8462, 8555, 8633 414 748 LYM1185 LYM1185 MAIZE Zea mays L. 8315,8463, 8556, 8634 415 749 LYM1186 LYM1186 MAIZE Zea mays L. 8316, 8464,8557, 8635 416 750 LYM1187 LYM1187 MAIZE Zea mays L. 8317, 8465, 8317,8465 417 751 LYM1188 LYM1188 RICE Oryza sativa L. 8318, 8466, 8558, 8636418 637 LYM1189 LYM1189_GA 419 638 LYM1190 LYM1190 RICE Oryza sativa L.8319, 8467, 8319, 8467 420 639 LYM1191 LYM1191 RICE Oryza sativa L 8320,8468, 8559, 8637 421 640 LYM1192 LYM1192 RICE Oryza sativa L. 8321,8469, 8321, 8469 422 641 LYM1193 LYM1193_GA 423 642 LYM1194 LYM1194_GA424 643 LYM1195 LYM1195 SORGHUM Sorghum bicolor 8322, 8470, 8322, 8470425 644 LYM1201 LYM1201_GA 426 645 LYM1202 LYM1202_GA 427 646 LYM1203LYM1203 SORGHUM Sorghum bicolor 8324, 8472, 8324, 8472 428 752 LYM1204LYM1204 SORGHUM Sorghum bicolor 8325, 8473, 8560, 8638 429 753 LYM1205LYM1205 SORGHUM Sorghum bicolor 8326, 8474, 8561, 8639 430 754 LYM1206LYM1206 SORGHUM Sorghum bicolor 8327, 8475, 8562, 8640 431 650 LYM1207LYM1207 SORGHUM Sorghum bicolor 8328, 8476, 8328, 8476 432 651 LYM1208LYM1208 SORGHUM Sorghum bicolor 8329, 8477, 8563, 8641 433 652 LYM1209LYM1209 SORGHUM Sorghum bicolor 8330, 8478, 8564, 8642 434 653 LYM1210LYM1210 SORGHUM Sorghum bicolor 8331, 8479, 8331, 8479 435 654 LYM1211LYM1211 SORGHUM Sorghum bicolor 8332, 8480, 8565, 8643 436 655 LYM1212LYM1212 SORGHUM Sorghum bicolor 8333, 8481, 8566, 8644 437 656 LYM1213LYM1213 SORGHUM Sorghum bicolor 8334, 8482, 8567, 8645 438 657 LYM1214LYM1214 SORGHUM Sorghum bicolor 8335, 8483, 8335, 8483 439 658 LYM1215LYM1215_GA 440 659 LYM1216 LYM1216 SOYBEAN Glycine max 8336, 8484, 8568,8646 441 660 LYM1217 LYM1217 SOYBEAN Glycine max 8337, 8485, 8337, 8485442 661 LYM1218 LYM1218_GA 443 662 LYM1219 LYM1219 SOYBEAN Glycine max8338, 8486, 8338, 8486 444 663 LYM1220 LYM1220 SOYBEAN Glycine max 8339,8487, 8339, 8487 445 664 LYM1221 LYM1221 SOYBEAN Glycine max 446 665LYM1223 LYM1223 SOYBEAN Glycine max 8341, 8489, 8569, 8647 447 667LYM1224 LYM1224 SOYBEAN Glycine max 8342, 8490, 8570, 8648 448 668LYM1225 LYM1225 SOYBEAN Glycine max 8343, 8491, 8571, 8649 449 669LYM1226 LYM1226 SOYBEAN Glycine max 8344, 8492, 8572, 8650 450 670LYM1227 LYM1227_GA 451 671 LYM1228 LYM1228_GA 452 672 LYM1229 LYM1229_GA453 673 LYM1230 LYM1230 TOMATO Lycopersicum 8345, 8493, 8573, 8651 454674 esculentum LYM1231 LYM1231 TOMATO Lycopersicum 8346, 8494, 8346,8494 455 755 esculentum LYM1232 LYM1232 TOMATO Lycopersicum 8347, 8495,8347, 8495 456 676 esculentum LYM1233 LYM1233 TOMATO Lycopersicum 8348,8496, 8348, 8496 457 677 esculentum LYM1234 LYM1234_GA 458 678 LYM1235LYM1235_GA 459 679 BRACHYPODIUM 8349, 8497, 8574, 8652 460 756 LYM1236LYM1236 Brachypodiums dis LYM1237 LYM1237 BRACHYPODIUM 8350, 8498, 8575,8653 461 757 Brachypodiums dis LYM1239 LYM1239_GA 462 682 LYM1240LYM1240 TOMATO Lycopersicum 8351, 8499, 8351, 8499 463 758 esculentumLYM1032_ LYM1032_H1 WHEAT Triticum aestivum L. 8219, 8367, 8219, 8367464 759 H1 LYM1058_ LYM1058_H4_ 465 685 H4 GA LYM1059_ LYM1059_H7_ 466686 H7 GA LYM1064_ LYM1064_H5_ 467 687 H5 GA LYM1076_ LYM1076_H4 RICEOryza sativa L. 8245, 8393, 8518, 8594 468 688 H4 LYM1091_ LYM1091_H5_H5 GA 469 689 LYM1101_ LYM1101_H3_ 470 690 H3 GA LYM1105_ LYM1105_H2_471 691 H2 GA LYM1164_ LYM1164_H1 SORGHUM Sorghum bicolor 8298, 8446,8298, 8446 472 760 H1 LYM1196 LYM1196 SORGHUM Sorghum bicolor 8323,8471, 8323, 8471 473 — Table 107. For cloning of each gene at least 2primers were used: Forward (Fwd) or Reverse (Rev). In some cases, 4primers were used: External forward (EF), External reverse (ER), nestedforward (NF) or nested reverse (NR). The sequences of the primers usedfor cloning the genes are provided in the sequence listing. Some geneswere synthetically produced by GeneArt (marked as “GA”).

Example 16 Transforming Agrobacterium tumefaciens Cells with BinaryVectors Harboring Putative Genes

The above described binary vectors were used to transform Agrobacteriumcells. Two additional binary constructs, having only the At6669 or the35S promoter, or no additional promoter were used as negative controls.

The binary vectors were introduced to Agrobacterium tumefaciens GV301 orLB4404 (for Arabidopsis) or AGL1 (for Brachypodium) competent cells(about 10⁹ cells/mL) by electroporation. The electroporation wasperformed using a MicroPulser electroporator (Biorad), 0.2 cm cuvettes(Biorad) and EC-2 electroporation program (Biorad). The treated cellswere cultured in LB liquid medium at 28° C. for 3 hours, then platedover LB agar supplemented with gentamycin (for Arabidopsis; 50 mg/L; forAgrobacterium strains GV301) or streptomycin (for Arabidopsis; 300 mg/L;for Agrobacterium strain LB4404); or with Carbenicillin (forBrachypodium; 50 mg/L) and kanamycin (for Arabidopsis and Brachypodium;50 mg/L) at 28° C. for 48 hours. Agrobacterium colonies, which weredeveloped on the selective media, were further analyzed by PCR using theprimers designed to span the inserted sequence in the pPI plasmid. Theresulting PCR products were isolated and sequenced to verify that thecorrect polynucleotide sequences of the invention were properlyintroduced to the Agrobacterium cells.

Example 17 Producing Transgenic Arabidopsis Plants Expressing SelectedGenes According to Some Embodiments of the Invention

Materials and Experimental Methods

Plant transformation—The Arabidopsis thaliana var Columbia (To plants)were transformed according to the Floral Dip procedure [Clough S J, BentA F. (1998) Floral dip: a simplified method for Agrobacterium-mediatedtransformation of Arabidopsis thaliana. Plant J. 16(6): 735-43; andDesfeux C, Clough S J, Bent A F. (2000) Female reproductive tissues werethe primary targets of Agrobacterium-mediated transformation by theArabidopsis floral-dip method. Plant Physiol. 123(3): 895-904] withminor modifications. Briefly, Arabidopsis thaliana Columbia (Col0) Toplants were sown in 250 ml pots filled with wet peat-based growth mix.The pots were covered with aluminum foil and a plastic dome, kept at 4°C. for 3-4 days, then uncovered and incubated in a growth chamber at18-24° C. under 16/8 hours light/dark cycles. The T₀ plants were readyfor transformation six days before anthesis.

Single colonies of Agrobacterium carrying the binary vectors harboringthe yield genes were cultured in LB medium supplemented with kanamycin(50 mg/L) and gentamycin (50 mg/L). The cultures were incubated at 28°C. for 48 hours under vigorous shaking and centrifuged at 4000 rpm for 5minutes. The pellets comprising Agrobacterium cells were resuspended ina transformation medium which contained half-strength (2.15 g/L)Murashige-Skoog (Duchefa); 0.044 μM benzylamino purine (Sigma); 112 pg/LB5 Gambourg vitamins (Sigma); 5% sucrose; and 0.2 ml/L Silwet L-77 (OSISpecialists, CT) in double-distilled water, at pH of 5.7.

Transformation of T₀ plants was performed by inverting each plant intoan Agrobacterium suspension such that the above ground plant tissue wassubmerged for 3-5 seconds. Each inoculated To plant was immediatelyplaced in a plastic tray, then covered with clear plastic dome tomaintain humidity and was kept in the dark at room temperature for 18hours to facilitate infection and transformation. Transformed(transgenic) plants were then uncovered and transferred to a greenhousefor recovery and maturation. The transgenic T₀ plants were grown in thegreenhouse for 3-5 weeks until siliques were brown and dry, then seedswere harvested from plants and kept at room temperature until sowing.

For generating T₁ and T₂ transgenic plants harboring the genes, seedscollected from transgenic T₀ plants were surface-sterilized by soakingin 70% ethanol for 1 minute, followed by soaking in 5% sodiumhypochlorite and 0.05% triton for 5 minutes. The surface-sterilizedseeds were thoroughly washed in sterile distilled water then placed onculture plates containing half-strength Murashig-Skoog (Duchefa); 2%sucrose; 0.8% plant agar; 50 mM kanamycin; and 200 mM carbenicylin(Duchefa). The culture plates were incubated at 4° C. for 48 hours thentransferred to a growth room at 25° C. for an additional week ofincubation. Vital T₁ Arabidopsis plants were transferred to a freshculture plates for another week of incubation. Following incubation theT₁ plants were removed from culture plates and planted in growth mixcontained in 250 ml pots. The transgenic plants were allowed to grow ina greenhouse to maturity. Seeds harvested from T₁ plants were culturedand grown to maturity as T2 plants under the same conditions as used forculturing and growing the T₁ plants.

Example 18 Transformation of Brachypodium distachyon Plants with thePolynucleotides of the Invention

Similar to the Arabidopsis model plant, Brachypodium distachyon hasseveral features that recommend it as a model plant for functionalgenomic studies, especially in the grasses. Traits that make it an idealmodel include its small genome (˜160 Mbp for a diploid genome and 355Mbp for a polyploidy genome), small physical stature, a short lifecycle,and few growth requirements. Brachypodium is related to the major cerealgrain species but is understood to be more closely related to theTriticeae (wheat, barley) than to the other cereals. Brachypodium, withits polyploidy accessions, can serve as an ideal model for these grains(whose genomics size and complexity is a major barrier tobiotechnological improvement).

Brachypodium distachyon embryogenic calli were transformed using theprocedure described by Vogel and Hill (2008) [High-efficiencyAgrobacterium-mediated transformation of Brachypodium distachyon inbredline Bd21-3. Plant Cell Rep 27:471-478], Vain et al (2008)[Agrobacterium-mediated transformation of the temperate grassBrachypodium distachyon (genotypeBd21) for T-DNA insertionalmutagenesis. Plant Biotechnology J 6: 236-245], and Vogel J. et al.(2006) [Agrobacterium mediated transformation and inbred linedevelopment in the model grass Brachypodium distachyon. Plant Cell TissOrg. Cult. 85:199-211], each of which is fully incorporated herein byreference, with some minor modifications, which are briefly summarizedhereinbelow.

Callus initiation—Immature spikes (about 2 months after seeding) wereharvested at the very beginning of seeds filling. Spikes were thenhusked and surface sterilized with 3% NaClO containing 0.1% Tween 20,shaked on a gyratory shaker at low speed for 20 minutes. Following threerinses with sterile distilled water, embryos were excised under adissecting microscope in a laminar flow hood using fine forceps.

Excised embryos (size ˜0.3 mm, bell shaped) were placed on callusinduction medium (CIM) [LS salts (Linsmaier, E. M. & Skoog. F. 1965.Physiol. Plantarum 18, 100) and vitamins plus 3% sucrose, 6 mg/L CuSO₄,2.5 mg/l 2,4-Dichlorophenoxyacetic Acid, pH 5.8 and 0.25% phytagel(Sigma)] scutellar side down, 100 embryos on a plate, and incubated at28° C. in the dark. Ten days later, the embryonic calli were cleanedfrom emerging roots, shoots and somatic calli, and was subcultured ontofresh CIM medium. During culture, yellowish embryogenic calli (EC)appeared and were further selected (e.g., picked and transferred) forfurther incubation in the same conditions for additional 2 weeks.Twenty-five pieces of sub-cultured calli were then separately placed on90×15 mm petri plates, and incubated as before for three additionalweeks.

Transformation—As described in Vogel and Hill (2008. Supra),Agrobacterium was scraped off 2-day-old MGL plates (plates with the MGLmedium which contains: Tryptone 5 g/l , Yeast Extract 2.5 g/l . NaCl 5g/l . D-Mannitol 5 g/l, MgSO₄*7H₂O 0.204 g/l, K₂HPO₄ 0.25 g/l , GlutamicAcid 1.2 g/l, Plant Agar 7.5 g/l) and resuspended in liquid MS mediumsupplemented with 200 μM acetosyringone to an optic density (OD) at 600nm (OD₆₀₀) of 0.6. Once the desired OD was attained, 1 ml of 10%Synperonic PE/F68 (Sigma) per 100 ml of inoculation medium was added.

To begin inoculation, 300 callus pieces were placed in approximately 12plates (25 callus pieces in each plate) and covered with theAgrobacterium suspension (8-8.5 ml). The calli were incubated in theAgrobacterium suspension for 15 minutes with occasional gentle rocking.After incubation, the Agrobacterium suspension was aspirated off and thecalli were then transferred into co-cultivation plates, prepared byplacing a sterile 7-cm diameter filter paper in an empty 90×15 mm petriplate. The calli pieces were then gently distributed on the filterpaper. One co-cultivation plate was used for two starting callus plates(50 initial calli pieces). The co-cultivation plates were then sealedwith saran wrap and incubated at 24° C. in the dark for 3 days.

The callus pieces were then individually transferred onto CIM medium asdescribed above, which was further supplemented with 200 mg/lTicarcillin (to kill the Agrobacterium) and Bialaphos (5 mg/L) (forselection of the transformed resistant embryogenic calli sections), andincubated at 28° C. in the dark for 14 days.

The calli pieces were then transferred to shoot induction media (SIM; LSsalts and vitamins plus 3% Maltose monohydrate) supplemented with 200mg/l Ticarcillin. Bialaphos (5 mg/L), Indol-3-acetic acid (IAA) (0.25mg/L), and 6-Benzylaminopurine (BAP) (1 mg/L), and were sub-cultured inlight to the same media after 10 days (total of 20 days). At eachsub-culture all the pieces from a single callus were kept together tomaintain their independence and were incubated under the followingconditions: lighting to a level of 60 IE m−2 s−1, a 16-h light, 8-h darkphotoperiod and a constant 24° C. temperature. Plantlets emerged fromthe transformed calli.

When plantlets were large enough to handle without damage, they weretransferred to plates containing the above mentioned shoot inductionmedia (SIM) with Bialaphos. Each plantlet was considered as a differentevent. The plantlets grew axillary tillers and eventually became bushy.Each bush from the same plant (event ID) was then divided to tissueculture boxes (“Humus”) containing “rooting medium” [MS basal salts, 3%sucrose, 3 g/L phytagel, 2 mg/l α-Naphthalene Acetic Acid (NAA) and 1mg/L IAA and Ticarcillin 200 mg/L, PH 5.8). All plants in a “Humus box”were different plants of the same transformation event. These plants aretested in bulk for expression of bar gene responsible for resistance toglufosinate using strips AgraStrip® LL. Romer Labs.

When plantlets established roots they were transplanted to soil andtransferred to a greenhouse. To verify the transgenic status of plantscontaining the other constructs, T0 plants were subjected to PCR aspreviously described by Vogel et al. 2006 [Agrobacterium mediatedtransformation and inbred line development in the model grassBrachypodium distachyon. Plant Cell Tiss Org. Cult. 85:199-211].

Example 19 Evaluation of Transgenic Arabidopsis for Seed Yield and PlantGrowth Rate Under Normal Conditions in Greenhouse Assays (GH-SM Assays)

Assay 1: Seed yield plant biomass and plant growth rate under normalgreenhouse conditions—This assay follows seed yield production, thebiomass formation and the rosette area growth of plants grown in thegreenhouse at non-limiting nitrogen growth conditions. TransgenicArabidopsis seeds were sown in agar media supplemented with ½ MS mediumand a selection agent (Kanamycin). The T₂ transgenic seedlings were thentransplanted to 1.7 trays filled with peat and perlite in a 1:1 ratio.The trays were irrigated with a solution containing 6 mM inorganicnitrogen in the form of KNO₃ with 1 mM KH₂PO₄, 1 mM MgSO₄, 2 mM CaCl₂)and microelements. All plants were grown in the greenhouse until matureseeds. Seeds were harvested, extracted and weighted. The remaining plantbiomass (the above ground tissue) was also harvested, and weightedimmediately or following drying in oven at 50° C. for 24 hours.

Each construct was validated at its T₂ generation. Transgenic plantstransformed with a construct conformed by an empty vector carrying theAt6669 promoter and the selectable marker were used as control.

The plants were analyzed for their overall size, growth rate, flowering,seed yield, 1,000-seed weight, dry matter and harvest index (HI-seedyield/dry matter). Transgenic plants performance was compared to controlplants grown in parallel under the same conditions. Mock-transgenicplants expressing the uidA reporter gene (GUS-Intron) or with no gene atall, under the same promoter were used as control.

The experiment was planned in nested randomized plot distribution. Foreach gene of the invention three to five independent transformationevents were analyzed from each construct.

Digital imaging—A laboratory image acquisition system, which consists ofa digital reflex camera (Canon EOS 300D) attached with a 55 mm focallength lens (Canon EF-S series), mounted on a reproduction device(Kaiser RS), which includes 4 light units (4×150 Watts light bulb) wasused for capturing images of plant samples.

The image capturing process was repeated every 2 days starting from day1 after transplanting till day 15. Same camera, placed in a custom madeiron mount, was used for capturing images of larger plants sawn in whitetubs in an environmental controlled greenhouse. The tubs were squareshape include 1.7 liter trays. During the capture process, the tubs wereplaced beneath the iron mount, while avoiding direct sun light andcasting of shadows.

An image analysis system was used, which consists of a personal desktopcomputer (Intel P4 3.0 GHz processor) and a public domain program—ImageJ1.39 [Java based image processing program which was developed at theU.S. National Institutes of Health and freely available on the internetat rsbweb (dot) nih (dot) gov/]. Images are captured in resolution of 10Mega Pixels (3888×2592 pixels) and stored in a low compression JPEG(Joint Photographic Experts Group standard) format. Next, analyzed datawas saved to text files and processed using the JMP statistical analysissoftware (SAS institute).

Leaf analysis—Using the digital analysis leaves data was calculated,including leaf number, rosette area, rosette diameter, and leaf bladearea.

Vegetative growth rate: the relative growth rate (RGR) of leaf number(Formula VIII), rosette area (Formula IX), plot coverage (Formula XI)and harvest index (Formula XV) was calculated with the indicatedformulas as described above.

Seeds average weight—At the end of the experiment all seeds werecollected.

The seeds were scattered on a glass tray and a picture is taken. Usingthe digital analysis, the number of seeds in each sample was calculated.

Dry weight and seed yield—On about day 80 from sowing, the plants wereharvested and left to dry at 30° C. in a drying chamber. The biomass andseed weight of each plot were measured and divided by the number ofplants in each plot.

Dry weight=total weight of the vegetative portion above ground(excluding roots) after drying at 30° C. in a drying chamber;

Seed yield per plant=total seed weight per plant (gr.).

1000 seed weight (the weight of 1000 seeds) (gr.).

Oil percentage in seeds—At the end of the experiment all seeds from eachplot were collected. Seeds from 3 plots were mixed grounded and thenmounted onto the extraction chamber. 210 ml of n-Hexane (Cat No. 080951Biolab Ltd.) were used as the solvent. The extraction was performed for30 hours at medium heat 50° C. Once the extraction has ended then-Hexane was evaporated using the evaporator at 35° C. and vacuumconditions. The process was repeated twice. The information gained fromthe Soxhlet extractor (Soxhlet, F. Die gewichtsanalytische Bestimmungdes Milchfettes, Polytechnisches J. (Dingler's) 1879, 232, 461) was usedto create a calibration curve for the Low Resonance NMR. The content ofoil of all seed samples was determined using the Low Resonance NMR(MARAN Ultra-Oxford Instrument) and its MultiQuant software package.

Silique length analysis—On day 50 from sowing, 30 siliques fromdifferent plants in each plot were sampled in block A. The chosensiliques were green-yellow in color and were collected from the bottomparts of a grown plant's stem. A digital photograph was taken todetermine silique's length.

Statistical analyses—To identify outperforming genes and constructs,results from the independent transformation events tested were analyzedseparately. Data was analyzed using Student's t-test and results wereconsidered significant if the p value was less than 0.1. The JMPstatistics software package was used (Version 5.2.1. SAS Institute Inc.,Cary. N.C. USA).

Tables 108-112 summarize the observed phenotypes of transgenic plantsexogenously expressing the gene constructs using the seed maturation(GH-SM) assays under normal conditions. The evaluation of each gene wasperformed by testing the performance of different number of events.Event with p-value <0.1 was considered statistically significant.

TABLE 108 Genes showing improved plant performance at Normal growthconditions under regulation of At6669 promoter Inflorescence Gene DryWeight [mg] Flowering Emergence Name Event # Ave. P-Val. % Incr. Ave.P-Val. % Incr. Ave. P-Val. % Incr. LYM1062 81476.3 — — — 33.5 L −6 — — —LYM1019 81663.3 — — — 32.1 0.10 −10 25.1 L −6 LYM1019 81665.1 — — — — —— 25.2 L −5 CONT. — — — — 35.7 — — 26.6 — — LYM1240 80614.1 — — — 37.00.15 −3 — — — LYM1240 80614.4 — — — 37.2 0.21 −3 — — — LYM1236 80505.4 —— — 36.8 0.13 −4 — — — LYM1236 80505.5 — — — 37.6 0.26 −2 — — — LYM122481497.3 — — — 35.7 L −7 27.5 L −7 LYM1224 81497.4 — — — 36.2 0.02 −5 — —— LYM1224 81498.3 — — — 37.5 0.22 −2 — — — LYM1224 81498.6 — — — 36.80.29 −4 — — — LYM1213 81340.3 — — — 37.4 0.21 −2 — — — LYM1206 81222.2 —— — 37.5 0.22 −2 — — — LYM1188 81028.4 — — — 37.3 0.13 −3 — — — LYM116580587.2 — — — 35.1 0.09 −8 27.3 0.05 −7 LYM1165 80588.3 — — — 36.5 0.02−5 — — — LYM1165 80590.3 — — — 37.2 0.30 −3 — — — LYM1165 80590.6 — — —37.1 0.13 −3 — — — LYM1159 80276.5 1915.6 0.03 29 — — — — — — LYM114380542.2 — — — 37.3 0.13 −3 — — — LYM1126 80935.2 — — — 37.6 0.27 −2 — —— LYM1089 810911 — — — 36.2 0.02 −5 — — — LYM1089 81095.1 1592.5 0.18 7— — — — — — LYM1079 81354.5 — — — 37.0 0.15 −3 — — — LYM1079 81355.51902.2 0.12 28 — — — — — — LYM1065 80968.3 — — — 37.3 0.16 −2 — — —LYM1062 81474.2 — — — 35 L −6 — — — LYM1062 81476.3 — — — 36.5 0.18 −5 —— — CONT. — 1488.7 — — 38.3 — — 29.4 — — LYM1240 80615.1 — — — — — —26.8 0.13 −2 LYM1240 80615.2 — — — — — — 26.8 0.07 −2 LYM1224 81497.4 —— — — — — 26.8 0.13 −2 LYM1224 81497.6 — — — 35.9 0.04 −5 26.4 0.19 −4LYM1224 81498.3 — — — 34.6 0.18 −9 25.5 L −7 LYM1224 81498.6 — — — — — —26.1 0.19 −5 LYM1206 81220.5 — — — 36.7 0.11 −3 26.9 0.15 −2 LYM120681223.3 — — — — — — 27.0 0.20 −2 LYM1190 80378.1 — — — 36.8 0.25 −3 26.70.04 −3 LYM1190 80378.7 — — — 37.0 L −2 26.9 0.15 −2 LYM1190 80379.3 — —— — — — 26.7 0.06 −3 LYM1188 81028.4 — — — — — — 27.0 0.20 −2 LYM118881030.1 — — — — — — 26.5 0.03 −3 LYM1181 80371.6 — — — — — — 26.8 0.13−2 LYM1181 80373.1 — — — — — — 26.3 0.09 −4 LYM1181 80373.6 — — — — — —26.8 0.07 −2 LYM1181 80373.8 — — — — — — 27.0 0.20 −2 LYM1171 80591.4 —— — — — — 26.9 0.15 −2 LYM1171 80594.1 — — — — — — 27.0 0.20 −2 LYM117180595.5 — — — — — — 26.5 0.03 −3 LYM1171 80595.7 — — — — — — 26.7 0.17−3 LYM1165 80587.2 — — — 34.6 0.19 −8 25.7 0.03 −6 LYM1165 80588.4 — — —36.8 0.01 −3 26.9 0.15 −2 LYM1165 80590.3 — — — 37.1 0.07 −2 26.9 0.15−2 LYM1165 80590.6 — — — 36.1 L −5 26.7 0.17 −3 LYM1159 80276.5 — — —36.8 L −3 26.3 0.09 −4 LYM1159 80276.6 — — — — — — 26.9 0.15 −2 LYM115681017.1 — — — 37.1 0.04 −2 26.9 0.15 −2 LYM1156 81019.1 — — — — — — 27.00.20 −2 LYM1143 80539.3 — — — — — — 26.9 0.15 −2 LYM1143 80540.1 — — — —— — 26.5 0.27 −3 LYM1143 80542.2 — — — — — — 27.0 0.20 −2 LYM113881477.5 — — — — — — 26.7 0.04 −3 LYM1138 81480.3 — — — — — — 26.9 0.15−2 LYM1126 80934.2 — — — — — — 26.9 0.15 −2 LYM1126 80934.5 — — — — — —27.0 0.20 −2 LYM1126 80935.2 — — — — — — 26.8 0.13 −2 LYM1126 80935.4 —— — — — — 27.1 0.29 −1 LYM1099 81215.5 — — — — — — 26.8 0.13 −2 LYM109981216.3 — — — — — — 27.0 0.20 −2 LYM1079 81354.5 — — — — — — 27.0 0.20−2 LYM1079 81355.5 — — — — — — 27.0 0.20 −2 LYM1079 81358.1 — — — — — —26.7 0.17 −3 LYM1079 81358.2 — — — 35.9 L −5 26.4 0.01 −4 LYM106881084.1 — — — — — — 27.0 0.20 −2 LYM1068 81085.1 — — — — — — 27.0 0.20−2 LYM1065 80968.3 — — — — — — 27.0 0.20 −2 LYM1065 80970.3 — — — — — —26.8 0.07 −2 LYM1065 80971.1 — — — — — — 26.6 0.09 −3 LYM1065 80971.3 —— — — — — 27.0 0.20 −2 LYM1065 80971.4 — — — 37.1 0.04 −2 27.0 0.20 −2CONT. — — — — 37.8 — — 27.4 — — LYM1171 80591.4 1804.4 0.18 23 — — — — —— LYM1171 80594.1 — — — — — — 27.1 0.07 −2 LYM1171 80595.3 — — — — — —27.2 0.12 −2 LYM1171 80595.7 — — — — — — 27.0 0.03 −3 LYM1167 80183.4 —— — — — — 27.2 0.12 −2 LYM1110 81005.2 — — — 34.7 0.04 −3 27.0 0.03 −3LYM1100 80988.5 — — — — — — 27.2 0.12 −2 LYM1092 81365.2 — — — 34.5 0.14−3 27.0 0.03 −3 LYM1092 81366.5 — — — — — — 27.1 0.05 −2 LYM1092 81366.6— — — — — — 27.2 0.17 −2 LYM1056 80339.1 — — — — — — 27.2 0.12 −2LYM1056 80339.3 — — — 35.2 0.22 −1 27.1 0.07 −2 LYM1056 80340.1 — — — —— — 27.0 0.03 −3 LYM1055 81145.2 1600.6 0.09 9 — — — — — — LYM105581146.3 — — — 35.2 0.22 −1 27.2 0.12 −2 LYM1049 80103.6 — — — — — — 27.20.12 −2 LYM1043 81236.1 — — — — — — 27.2 0.12 −2 LYM1043 81237.4 — — — —— — 27.1 0.05 −2 LYM1043 81238.2 — — 34.7 0.23 −3 27.1 0.05 −2 LYM104180957.4 1693.8 0.03 15 — — — — — — LYM1041 80961.2 — — — — — — 27.0 0.03−3 LYM1040 80952.5 — — — — — — 27.0 0.03 −3 LYM1040 80956.1 — — — 35.20.22 −1 — — — LYM1034 80100.6 — — — — — — 27.0 0.03 −3 LYM1019 81663.2 —— — — — — 27.0 0.03 −3 LYM1019 81665.1 — — — — — — 27.0 0.03 −3 LYM101981666.1 — — — — — — 27.2 0.12 −2 LYM1019 81666.2 — — — 34.6 0.17 −3 27.10.05 −2 LYM1019 81666.3 — — — — — — 27.2 0.12 −2 LYM1010 81063.2 — — —34.6 0.25 −3 27.1 0.05 −2 LYM1010 81064.2 1586.9 0.18 8 — — — — — —CONT. — 1469.5 — — 35.6 — — 27.7 — — Table 108: ″CONT.″ - Control;″Ave.″ - Average; ″% Incr.″ = % increment; ″p-val.″ - p-value, L-p <0.01.

It should be noted that a negative increment (in percentages) when foundin flowering or inflorescence emergence indicates drought avoidance ofthe plant.

TABLE 109 Genes showing improved plant performance at Normal growthconditions under regulation of At6669 promoter Leaf Blade Gene Area[cm²] Leaf Number Plot Coverage [cm²] Name Event # Ave. P-Val. % Incr.Ave. P-Val. % Incr. Ave. P-Val. % Incr. LYM1062 81474.2 — — — 9.19 0.083 — — — LYM1062 81476.3 0.814 0.29 8 — — — 43.4 0.05 9 LYM1061 80111.1 —— — 9.44 L 5 — — — LYM1061 80111.3 — — — 9.12 0.12 2 — — — LYM106180112.1 — — — 9.38 L 5 — — — LYM1061 80112.4 0.824 0.30 9 — — — — — —LYM1061 80115.2 — — — 9.38 0.09 5 — — — LYM1056 80339.1 0.804 0.15 7 — —— 41.9 0.17 6 LYM1056 80339.3 — — — — — — 43.8 0.14 10 LYM1055 81146.3 —— — 9.31 0.26 4 — — — LYM1049 80104.3 — — — 10.2 0.26 14 — — — LYM104980104.5 — — — 9.44 0.18 5 — — — LYM1043 81234.1 — — — 9.44 L 5 — — —LYM1043 81236.1 — — — 9.31 0.26 4 — — — LYM1043 81238.2 — — — 9.56 L 7 —— — LYM1041 80957.2 — — — 9.56 L 7 — — — LYM1041 80960.2 0.821 0.04 9 —— — 43.0 0.05 8 LYM1041 80961.1 — — — 9.19 0.08 3 — — — LYM1038 80326.4— — — 9.38 0.09 5 — — — LYM1038 80327.2 — — — 9.25 0.02 3 — — — LYM103681078.1 — — — 9.38 L 5 44.9 0.18 13 LYM1036 81080.6 — — — 9.38 0.09 5 —— — LYM1025 80091.4 0.788 0.22 5 — — — — — — LYM1025 80094.3 — — — 9.310.02 4 — — — LYM1025 80094.4 — — — 9.50 L 6 — — — LYM1019 81663.3 0.937L 24 9.81 0.08 10 51.9 0.02 31 LYM1019 81665.1 0.797 0.13 6 — — — 42.60.08 7 LYM1019 81666.2 0.830 0.05 10 — — — 45.0 0.01 13 CONT. — 0.754 —— 8.95 — — 39.7 — — LYM1240 80615.2 0.940 L 12 10.0 0.20 7 50.0 0.22 13LYM1236 80505.2 — — — — — — 46.7 0.11 6 LYM1236 80505.5 0.965 L 15 — — —50.2 0.03 13 LYM1236 80506.5 — — — — — — 49.0 L 11 LYM1224 81497.4 0.965L 15 9.62 0.21 3 53.5 L 21 LYM1224 81497.6 0.982 L 17 9.81 0.19 5 53.70.21 21 LYM1224 81498.3 1.03 L 22 9.62 0.21 3 56.0 L 27 LYM1224 81498.61.10 L 31 — — — 62.4 0.10 41 LYM1213 81340.3 0.877 0.19 4 — — — — — —LYM1213 81340.4 0.941 0.09 12 — — — 50.7 0.27 15 LYM1213 81343.3 — — —10.1 0.24 8 — — — LYM1213 81343.4 1.02 0.23 21 — — — 53.9 0.2.7 22LYM1206 81220.5 0.921 0.02 10 — — — 49.4 0.07 12 LYM1188 81028.3 — — —10.1 0.02 8 — — — LYM1179 80243.1 0.933 L 11 9.94 L 6 51.1 0.01 16LYM1179 80243.4 — — — 10.4 0.20 11 — — — LYM1179 80243.6 0.990 L 18 — —— 51.3 0.13 16 LYM1179 80245.1 0.981 0.15 17 — — — 52.1 0.13 18 LYM117980245.3 0.891 0.10 6 — — — 46.2 0.21 4 LYM1165 80587.2 0.997 0.04 1910.1 0.10 8 55.3 L 25 LYM1165 80588.3 1.04 0.01 24 10.1 0.16 8 58.7 0.0533 LYM1165 80588.4 — — — 10.1 0.24 8 51.5 0.16 16 LYM1165 80590.3 — — —9.94 L 6 50.7 0.05 15 LYM1165 80590.6 0.951 0.16 13 — — — 51.8 0.20 17LYM1159 80276.5 1.01 0.19 20 — — — 57.6 0.24 30 LYM1159 80279.2 — — —9.69 0.29 4 — — — LYM1143 80539.1 0.956 L 14 — — — 50.6 L 14 LYM114380540.1 0.876 0.20 4 — — — — — — LYM1143 80541.3 0.919 0.04 9 — — — 51.4L 16 LYM1143 80542.1 0.880 0.26 5 9.56 0.14 2 46.7 0.14 6 LYM112680934.2 — — — 9.56 0.14 2 — — — LYM1126 80935.3 — — — 9.50 0.22 2 — — —LYM1126 80935.5 — — — 9.75 L 4 — — — LYM1099 81215.3 0.911 0.06 8 — — —48.6 0.01 10 LYM1099 81217.3 — — — 9.50 0.22 2 — — — LYM1089 81093.10.909 0.05 8 — — — — — — LYM1089 81095.1 1.06 0.04 25 — — — 58.5 L 32LYM1079 81355.5 1.01 0.02 20 9.88 L 6 55.6 L 26 LYM1079 81358.2 — — — —— — 48.3 0.28 9 LYM1065 80968.3 0.926 0.04 10 — — — 50.2 L 14 LYM106580970.3 0.911 0.11 8 — — — — — — LYM1065 80971.3 0.908 0.03 8 — — — 50.20.16 14 LYM1062 81474.2 1.13 0.01 34 10.7 0.04 14 66.2 0.10 50 LYM106281474.5 1.01 L 20 — — — 55.3 0.10 25 LYM1062 81476.2 — — — — — — 48.50.07 10 LYM1062 81476.3 1.07 0.05 27 10.4 0.30 12 59.7 0.13 35 CONT. —0.841 — — 9.36 — — 44.2 — — LYM1240 80614.4 1.21 0.02 12 10.9 L 14 70.80.02 19 LYM1240 80615.1 1.29 0.30 19 10.9 0.13 15 — — — LYM1224 81497.3— — — 10.1 0.08 6 — — — LYM1224 81497.4 1.19 0.03 10 — — — 67.8 0.06 14LYM1224 81497.6 1.29 0.05 19 — — — 75.4 0.26 27 LYM1224 81498.3 1.33 L23 10.6 L 11 79.7 L 34 LYM1224 81498.6 1.31 0.06 21 — — — 77.4 0.05 31LYM1206 81220.5 1.19 0.05 9 10.4 0.02 9 68.5 0.03 16 LYM1190 80378.11.33 L 23 11.6 L 22 82.0 L 38 LYM1190 80378.7 1.21 0.04 11 — — — 69.20.17 17 LYM1181 80371.6 1.32 L 22 10.4 0.02 9 75.8 L 28 LYM1181 80373.11.13 0.28 4 10.5 0.01 10 66.7 0.06 12 LYM1171 80591.4 — — — 10.2 0.04 7— — — LYM1171 80594.1 1.14 0.25 5 10.2 0.09 7 64.6 0.22 9 LYM117180595.7 — — — 10.3 0.28 8 — — — LYM1165 80587.2 1.20 0.06 11 9.94 0.26 468.5 0.02 15 LYM1165 80588.4 1.27 0.06 17 10.0 0.14 5 73.9 0.13 25LYM1165 80590.6 1.26 L 16 10.2 0.09 7 71.7 0.01 21 LYM1159 80276.5 1.38L 27 10.8 0.22 13 84.5 L 43 LYM1159 80276.6 — — — 10.1 0.15 6 — — —LYM1159 80279.2 — — — 10.4 0.02 9 — — — LYM1156 81017.1 1.27 L 17 9.940.26 4 70.2 0.03 18 LYM1156 81017.4 1.15 0.28 6 10.1 0.08 6 65.2 0.13 10LYM1143 80539.3 — — — 10.1 0.07 6 69.1 0.26 16 LYM1143 80540.1 1.21 0.0711 10.2 0.09 7 71.1 L 20 LYM1138 81477.5 1.19 0.12 10 10.4 0.03 9 72.8 L23 LYM1138 81480.1 1.44 0.15 33 10.7 0.08 12 86.7 0.09 46 LYM112680935.2 — — — 10.0 0.11 5 — — — LYM1099 81215.3 — — — 9.88 0.27 4 — — —LYM1099 81215.5 — — — 10.2 0.22 7 — — — LYM1079 81354.5 1.15 0.23 6 — —— 65.3 0.09 10 LYM1079 81358.2 1.17 0.10 8 10.0 0.14 5 — — — LYM106580970.3 — — — 10.0 0.14 5 66.6 0.19 12 LYM1065 80971.1 — — — — — — 64.60.15 9 LYM1065 80971.3 — — — 10.2 0.22 7 63.8 0.25 8 CONT. — 1.08 — —9.54 — — 59.3 — — LYM1171 80591.4 1.20 0.21 9 — — — 71.4 0.16 17 LYM116780183.2 — — — 10.4 0.19 2 — — — LYM1061 80114.2 — — — 10.8 0.03 6 67.00.18 9 LYM1056 80339.3 — — — — — — 67.9 0.28 11 LYM1043 81236.1 1.230.04 12 — — — 74.0 0.01 21 LYM1043 81238.2 1.32 0.03 21 — — — 75.6 0.0523 LYM1040 80952.5 1.20 0.14 9 — — — 70.1 0.05 14 LYM1036 81078.1 1.300.03 18 — — — 70.1 0.25 14 LYM1019 81663.2 — — — 10.8 0.23 7 70.1 0.0714 LYM1019 81665.1 — — — 10.8 L 6 71.0 0.08 16 LYM1019 81666.3 1.19 0.139 — — — 67.9 0.10 11 LYM1010 81063.4 1.20 0.11 9 10.9 L 8 68.9 0.06 12CONT. — 1.10 — — 10.1 — — 61.3 — — Table 109. ″CONT.″ - Control;″Ave.″ - Average; ″% Incr.″ = % increment; ″p-val.″ - p-value, L-p <0.01.

TABLE 110 Genes showing improved plant performance at Normal growthconditions under regulation of At6669 promoter RGR Of Leaf RGR Of PlotRGR Of Rosette Number Coverage Diameter Gene P- % P- % P- % Name Event #Ave. Val. Incr. Ave. Val. Incr. Ave. Val. Incr. LYM1062 81475.2 0.6490.21 18 — — — — — — LYM1061 80111.1 — — — — — — 0.354 0.25  9 LYM106180112.4 — — — — — — 0.358 0.18 10 LYM1056 80339.3 — — — — — — 0.368 0.0913 LYM1055 81146.3 0.617 0.29 12 — — — — — — LYM1049 80104.3 0.720 0.0331 — — — — — — LYM1049 80104.5 0.643 0.18 17 — — — — — — LYM1043 81238.20.628 0.21 14 — — — 0.353 0.27  8 LYM1041 80957.2 0.666 0.10 21 — — — —— — LYM1038 80328.2 0.639 0.22 16 — — — — — — LYM1025 80094.3 0.624 0.2814 — — — — — — LYM1025 80094.5 0.621 0.24 13 — — — — — — LYM1019 81663.30.638 0.18 16 6.49 0.06 29 — — — LYM1019 81665.1 0.652 0.16 19 — — — — —— LYM1019 81666.2 — — — — — — 0.357 0.18 10 CONT. — 0.549 — — 5.02 — —0.326 — — LYM1240 80615.2 0.663 0.21 23 5.92 0.28 14 — — — LYM123680505.4 0.661 0.19 23 — — — — — — LYM1236 80505.5 — — — 6.02 0.21 150.317 0.19 12 LYM1224 81497.4 — — — 6.19 0.13 19 — — — LYM1224 81497.6 —— — 6.34 0.09 22 0.311 0.27 10 LYM1224 81498.3 — — — 6.65 0.03 28 0.3240.10 14 LYM1224 81498.6 — — — 7.50 L 44 0.344 0.02 21 LYM1213 81340.4 —— — 6.08 0.18 17 — — — LYM1213 81343.4 — — — 6.30 0.09 21 0.313 0.24 10LYM1188 81028.3 0.654 0.21 22 — — — — — — LYM1179 80243.1 — — — 6.050.18 16 — — — LYM1179 80243.4 0.688 0.14 28 — — — — — — LYM1179 80243.6— — — 6.06 0.20 16 0.318 0.16 12 LYM1179 80245.1 — — — 6.16 0.14 18 — —— LYM1165 80587.2 0.652 0.22 21 6.62 0.03 27 0.334 0.05 18 LYM116580588.3 0.652 0.22 21 6.99 L 34 0.326 0.08 15 LYM1165 80588.4 — — — 6.070.20 16 — — — LYM1165 80590.3 — — — 5.98 0.23 15 — — — LYM1165 80590.6 —— — 6.11 0.16 17 0.311 0.27 10 LYM1159 80276.5 0.711 0.08 32 6.76 0.0330 — — — LYM1143 80539.1 — — — 6.06 0.18 16 0.310 0.27  9 LYM114380541.3 — — — 6.03 0.20 16 — — — LYM1089 81095.1 — — — 6.93 0.01 330.319 0.14 13 LYM1079 81355.5 — — — 6.60 0.04 27 0.318 0.16 12 LYM106580968.3 — — — 5.91 0.27 14 — — — LYM1065 80970.3 — — — 6.04 0.21 160.309 0.29  9 LYM1065 80971.3 — — — 5.88 0.28 13 — — — LYM1062 81474.20.671 0.15 25 7.80 L 50 0.372 L 31 LYM1062 81474.5 — — — 6.51 0.05 250.325 0.09 15 LYM1062 81476.3 0.692 0.11 29 7.09 L 36 0.346 0.02 22CONT. — 0.538 — — 5.21 — — 0.283 — — LYM1240 80614.1 0.620 0.16 14 — — —— — — LYM1240 80614.4 0.667 0.02 23 7.09 0.19 20 0.400 0.18 11 LYM124080615.1 0.660 0.05 22 7.34 0.13 25 0.404 0.17 12 LYM1224 81497.4 — — —6.81 0.30 16 — — — LYM1224 81497.6 0.614 0.22 13 7.45 0.11 27 — — —LYM1224 81498.3 — — — 7.88 0.04 34 0.414 0.08 15 LYM1224 81498.6 0.6430.08 19 7.64 0.07 30 — — — LYM1206 81220.5 0.614 0.17 13 — — — — — —LYM1190 80378.1 0.709 L 31 8.11 0.02 38 0.412 0.09 15 LYM1190 80378.7 —— — 6.86 0.28 17 — — — LYM1188 81030.1 0.633 0.13 17 — — — — — — LYM118180371.6 0.611 0.19 13 7.48 0.09 27 0.401 0.19 12 LYM1181 80373.1 0.6530.04 21 — — — 0.403 0.16 12 LYM1165 80587.2 0.608 0.24 12 6.88 0.26 170.392 0.28  9 LYM1165 80588.4 — — — 7.33 0.12 24 0.394 0.28 10 LYM116580590.3 0.619 0.15 14 — — — — — — LYM1165 80590.6 — — — 7.09 0.19 200.393 0.26  9 LYM1159 80276.5 0.625 0.16 15 8.42 0.01 43 0.445 L 24LYM1159 80279.2 0.606 0.22 12 — — — — — — LYM1156 81017.1 — — — 7.030.20 19 0.406 0.14 13 LYM1143 80539.3 — — — 6.93 0.25 18 0.396 0.23 10LYM1143 80540.1 — — — 7.09 0.18 20 — — — LYM1138 81477.5 0.610 0.19 127.23 0.15 23 0.398 0.21 11 LYM1138 81480.1 0.624 0.14 15 8.69 L 47 0.4330.02 21 LYM1065 80971.3 0.616 0.18 14 — — — — — — CONT. — 0.542 — — 5.89— — 0.359 — — LYM1171 80591.4 — — — 8.48 0.27 17 0.368 0.22 13 LYM117180595.3 0.765 0.17 15 — — — — — — LYM1167 80182.1 — — — — — — 0.370 0.1814 LYM1110 81005.2 0.750 0.22 13 — — — — — — LYM1056 80339.3 0.777 0.1317 — — — 0.365 0.24 12 LYM1055 81146.3 0.765 0.18 15 — — — — — — LYM104381236.1 — — — 8.88 0.14 22 0.370 0.17 14 LYM1043 81238.2 — — — 9.04 0.1125 0.386 0.09 19 LYM1036 81078.1 — — — 8.42 0.29 16 0.378 0.11 16LYM1034 80100.2 0.747 0.28 12 — — — — — — LYM1019 81663.2 — — — — — —0.377 0.12 16 LYM1019 81665.1 — — — 8.46 0.27 17 0.373 0.15 14 LYM101981666.3 — — — — — — 0.368 0.20 13 CONT. — 0.667 — — 7.25 — — 0.325 — —“CONT.” - Control; “Ave.” - Average; “% Incr.” = % increment; “p-val.” -p-value, L- p <0.01. RGR = relative growth rate.

TABLE 111 Genes showing improved plant performance at Normal growthconditions under regulation of At6669 promoter Rosette Diameter HarvestIndex Rosette Area [cm²] [cm] Gene P- % P- % P- % Name Event # Ave. Val.Incr. Ave. Val. Incr. Ave. Val. Incr. LYM1062 81476.3 — — — 5.43 0.05  93.94 0.03  6 LYM1061 80112.4 — — — — — — 4.02 0.14  8 LYM1056 80339.1 —— — 5.24 0.17  6 3.91 0.07  5 LYM1056 80339.3 — — — 5.48 0.14 10 4.070.05  9 LYM1041 80960.2 — — — 5.37 0.05  8 3.89 0.10  5 LYM1038 80330.4— — — — — — 3.92 0.20  5 LYM1036 81078.1 — — — 5.62 0.18 13 3.93 0.16  6LYM1036 81080.6 — — — — — — 3.85 0.19  3 LYM1019 81663.3 — — — 6.49 0.0231 4.18 L 12 LYM1019 81665.1 — — — 5.32 0.08  7 3.93 0.05  5 LYM101981666:2 — — — 5.62 0.01 13 4.07 L  9 LYM1019 81666.3 — — — — — — 3.930.15  5 CONT. — — — — 4.97 — — 3.72 — — LYM1240 80614.4 0.188 0.23 32 —— — — — — LYM1240 80615.2 — — — 6.25 0.22 13 4.16 0.26  6 LYM123680505.2 — — — 5.84 0.11  6 4.04 0.28  3 LYM1236 80505.5 0.171 0.02 206.27 0.03 13 4.27 L  9 LYM1236 80506.5 0.158 0.15 10 6.13 L 11 4.17 L  6LYM1224 81497.3 0.210 0.09 47 — — — — — — LYM1224 81497.4 0.194 0.26 366.69 L 21 4.29 0.06  9 LYM1224 81497.6 0.179 0.07 26 6.71 0.21 21 4.360.07 11 LYM1224 81498.3 0.173 0.28 21 7.00 L 27 4.45 L 13 LYM122481498.6 — — — 7.80 0.10 41 4.67 0.09 19 LYM1213 81340.4 0.167 0.04 176.34 0.27 15 4.23 0.12  8 LYM1213 81343.4 — — — 6;73 0:27 22 4.42 0.2112 LYM1206 81220.5 — — — 6.18 0.07 12 4.11 0.07  5 LYM1179 80243.1 — — —6.39 0.01 16 4.14 0.03 — LYM1179 80243.6 — — — 6.41 0.13 16 4.35 L 11LYM1179 80245.1 — — — 6.52 0.13 18 4.19 0.01  7 LYM1179 80245.3 — — —5.78 0.21  4 — — — LYM1165 80587.2 0.196 0.25 37 6.91 L 25 4.43 0.07 13LYM1165 80588.3 0.207 L 45 7.34 0.05 33 4.46 L 14 LYM1165 80588.4 — — —6.44 0.16 16 — — — LYM1165 80590.3 — — — 6.34 0.05 15 4.21 0.29  7LYM1165 80590.6 — — — 6.48 0.20 17 4.20 0.21  7 LYM1159 80276.5 — — —7.21 0.24 30 4.45 0.19 13 LYM1143 80539.1 — — — 6.32. L 14 4.24 L  8LYM1143 80541.3 — — — 6.42 L 16 4.18 L  7 LYM1143 80542.1 0.158 0.24 115.84 0.14  6 4.11 0.12 — LYM1126 80934.2 0.157 0.17 10 — — — — — —LYM1099 81215.3 — — — 6.07 0.01 10 4.11 0.02  5 LYM1089 81093.1 0.195 L37 — — — 4.10 0.15  5 LYM1089 81095.1 — — — 7.31 L 32 4.46 0.04 14LYM1079 81354.5 0.199 0.13 39 — — — — — — LYM1079 81355.5 — — — 6.95 L26 4.35 0.02 11 LYM1079 81358.2 — — — 6.03 0.28  9 4.13 0.23  5 LYM106580968.3 0.154 0.24 8 6.28 L 14 4.11 0.19  5 LYM1065 80970.5 0.164 0.1315 — — — — — — LYM1065 80971.3 — — — 6.28 0.16 14 4.13 0.10  5 LYM106281474.2 0.209 0.04 47 8.27 0.10 50 4.88 0.02  2 LYM1062 81474.5 — — —6.91 0.10 25 4.45 L 13 LYM1062 81476.2 0.178 0.06 25 6.06 0.07 10 4.110.09  5 LYM1062 81476.3 0.200 0.04 40 7.46 0.13 35 4.62 0.04 18 CONT. —0.143 — — 5.53 — — 3.93 — — LYM1240 80614.1 — — — — — — 4.80 0.28  4LYM1240 80614.4 — — — 8.85 0.02 19 5.05 0.04  9 LYM1240 80615.1 — — — —— — 5.20 0.27 12 LYM1224 81497.4 — — — 8.47 0.06 14 5.02 0.02 LYM122481497.6 — — — 9.42 0.26 27 — — — LYM1224 81498.3 — — — 9.97 L 34 5.42 L17 LYM1224 81498.6 — — — 9.68 0.05 31 5.18 L 12 LYM1206 81220.5 — — —8.57 0.03 16 4.77 0:22  3 LYM1190 80378.1 — — — 10.3 L 38 5.45 L 18LYM1190 80378.7 — — — 8.65 0.17 17 5.04 0.19  9 LYM1181 80371.6 — — —9.48 L 28 5.27 L 14 LYM1181 80373.1 — — — 8.34 0.06 12 5.05 0.09  9LYM1171 80594.1 — — — 8.08 0.22  9 — — — LYM1165 80587.2 — — — 8.56 0.0215 5.11 L 10 LYM1165 80588.4 — — — 9.24 0.13 25 5.17 L 12 LYM116580590.6 — — — 8.97 0.01 21 5.08 0.01 10 LYM1159 80276.5 — — — 10.6 L 435.67 L 23 LYM1156 81017.1 — — — 8.78 0.03 18 5.19 L 12 LYM1156 81017.4 —— — 8.15 0.13 10 4.83 0.09  4 LYM1143 80539.3 — — — 8.63 0.26 16 5.060.21  9 LYM1143 80540.1 — — — 8.89 L 20 4.98 0.01  8 LYM1138 81477.5 — —— 9.10 L 23 5.12 L 11 LYM1138 81480.1 — — — 10.8 0.09 46 5.57 0.14 20LYM1079 81354.5 — — — 8.16 0.09 10 4.82 0.10  4 LYM1079 81358.2 — — — —— — 4.95 0.18  7 LYM1065 80970.3 — — — 8.32 0.19 12 4.89 0.17  6 LYM106580971.1 — — — 8.08 0.15  9 — — — LYM1065 80971.3 — — — 7.97 0.25  8 4.810.15  4 CONT. — — — — 7.41 — — 4.63 — — LYM1171 80591.4 — — — 8.93 0.1617 4.87 0.20  9 LYM1171 80594.1 — — — — — — 4.66 0.13  4 LYM1171 80595.80.353 0.20 11 — — — — — — LYM1110 81003.1 0.363 0.11 15 — — — — — —LYM1110 81004.2 0.460 0.17 45 — — — — — — LYM1110 81006.2. 0.422 0.19 33— — — — — — LYM1092 81367.1 0.499 L 58 — — — — — — LYM1061 80114.2 — — —8.37 0.18  9 4.75 0.04  6 LYM1056 80339.3 — — — 8.49 0.28 11 4.77 0.09 7 LYM1056 80340.1 0.368 0.09 16 — — — — — LYM1043 81236.1 — — — 9.250.01 21 4.83 0.02  8 LYM1043 81238.2 — — — 9.45 0.05 23 5.15 0.16 15LYM1041 80957.2 — — — — — — 4.65 0.27  4 LYM1040 80952.5 — — — 8.76 0.0514 4.75 0.14  6 LYM1040 80955.2 0.494 0.14 56 — — — — — — LYM103681078.1 — — — 8.77 0.25 14 4.98 0.03 12 LYM1036 81078.2 — — — — — — 4.630.15  4 LYM1019 81663.2 — — — 8.76 0.07 14 4.84 0.02  8 LYM1019 81665.1— — — 8.88 0.08 16 4.94 L 11 LYM1019 81666.3 — — — 8.49 0.10 11 4.860.04  9 LYM1010 810614 — — — 8.61 0.06 12 4.88 L  9 CONT. — 0.317 — —7.66 — — 4.46 — — “CONT.” - Control; “Ave.” - Average; “% Incr.” = %increment; “p-val.” - p-value, L- p <0.01,

TABLE 112 Genes showing improved plant performance at Normal growthconditions under regulation of At666.9 promoter Gene Seed Yield [mg]1000 Seed Weight [mg] Name Event # Ave. P-Val. % Incr. Ave. P-Val. %Incr. LYM1224 81497.3 308.5 0.07 44 — — — LYM1224 81497.4 237.3 0.23 11— — — LYM1224 81497.6 237.7 0.29 11 — — — LYM1213 81340.4 241.2 0.24 13— — — LYM1165 80587.2 292.7 0.09 37 — — — LYM1165 80588.3 247.7 0.11 16— — — LYM1099 81215.2 287.0 0.20 34 — — — LYM1089 81093.1 236.7 0.25 11— — — LYM1079 81354.5 242.0 0.16 13 — — — LYM1062 81474.2 286.8 0.02 34— — — LYM1062 81476.2 260.3 0.15 22 — — — LYM1062 81476.3 251.3 0.21 17— — — CONT. — 214.0 — — — — — LYM1171 80591.4 — — — 27.7 0.02 20 LYM117180594.1 — — — 24.8 0.12  7 LYM1171 80595.8 — — — 24.0 0.04  4 LYM116780182.1 — — — 25.9 0.18 12 LYM1110 81005.2 — — — 24.9 0.08  8 LYM111081006.2 522.2 0.16 14 — — — LYM1056 80338.5 — — — 27.8 L 20 LYM105581146.3 — — — 24.2 0.12  5 LYM1040 80952.5 — — — 26.8 0.05 16 LYM102580091.1 — — — 24.2 0.03  5 LYM1010 81062.1 521.9 0.16 14 — — — LYM101081063.4 — — — 25.9 L 12 CONT. — 457.9 — — 23.1 — — “CONT.” - Control;“Ave.” - Average; “% Incr.” = % increment; “p-val.” - p-value L- p<0.01.

Example 20 Evaluation of Transgenic Arabidopsis for Seed Yield and PlantGrowth Rate Under Normal Conditions in Greenhouse Assays Until Bolting(GH-SB Assays)

Assay 2: Plant performance improvement measured until bolting stage:plant biomass and plant growth rate under normal greenhouse conditions(GH-SB Assays)—This assay follows the plant biomass formation and therosette area growth of plants grown in the greenhouse under normalgrowth conditions. Transgenic Arabidopsis seeds were sown in agar mediasupplemented with ½ MS medium and a selection agent (Kanamycin). The T₂transgenic seedlings were then transplanted to 1.7 trays filled withpeat and perlite in a 1:1 ratio. The trays were irrigated with asolution containing of 6 mM inorganic nitrogen in the form of KNO₃ with1 mM KH₂PO₄. 1 mM MgSO₄. 2 mM CaCl₂) and microelements. All plants weregrown in the greenhouse until bolting. Plant biomass (the above groundtissue) was weighted in directly after harvesting the rosette (plantfresh weight [FW]). Following plants were dried in an oven at 50° C. for48 hours and weighted (plant dry weight [DW]).

Each construct was validated at its T₂ generation. Transgenic plantstransformed with a construct conformed by an empty vector carrying theAt6669 promoter.

The plants were analyzed for their overall size, growth rate, freshweight and dry matter. Transgenic plants performance was compared tocontrol plants grown in parallel under the same conditions.Mock-transgenic plants expressing the uidA reporter gene (GUS-Intron) orwith no gene at all, under the same promoter were used as control.

The experiment was planned in nested randomized plot distribution. Foreach gene of the invention three to five independent transformationevents were analyzed from each construct.

Digital imaging—A laboratory image acquisition system, which consists ofa digital reflex camera (Canon EOS 300D) attached with a 55 mm focallength lens (Canon EF-S series), mounted on a reproduction device(Kaiser RS), which includes 4 light units (4×150 Watts light bulb) wasused for capturing images of plant samples.

The image capturing process was repeated every 2 days starting from day1 after transplanting till day 15. Same camera, placed in a custom madeiron mount, was used for capturing images of larger plants sawn in whitetubs in an environmental controlled greenhouse. The tubs were squareshape include 1.7 liter trays. During the capture process, the tubeswere placed beneath the iron mount, while avoiding direct sun light andcasting of shadows.

An image analysis system was used, which consists of a personal desktopcomputer (Intel P4 3.0 GHz processor) and a public domain program—ImageJ1.39 [Java based image processing program which was developed at theU.S. National Institutes of Health and freely available on the internetat rsbweb (dot) nih (dot) gov/]. Images were captured in resolution of10 Mega Pixels (3888×2592 pixels) and stored in a low compression JPEG(Joint Photographic Experts Group standard) format. Next, analyzed datawas saved to text files and processed using the JMP statistical analysissoftware (SAS institute).

Leaf analysis—Using the digital analysis leaves data was calculated,including leaf number, rosette area, rosette diameter, and leaf bladearea.

Vegetative growth rate: the relative growth rate (RGR) of leaf number(Formula VIII), rosette area (Formula IX) and plot coverage (Formula XI)were calculated using the indicated formulas as described above.

Plant Fresh and Dry weight—On about day 80 from sowing, the plants wereharvested and directly weighted for the determination of the plant freshweight (.FW) and left to dry at 50° C. in a drying chamber for about 48hours before weighting to determine plant dry weight (DW).

Statistical analyses—To identify outperforming genes and constructs,results from the independent transformation events tested were analyzedseparately. Data was analyzed using Student's t-test and results wereconsidered significant if the p value was less than 0.1. The JMPstatistics software package was used (Version 5.2.1, SAS Institute Inc.,Cary, N.C. USA).

Experimental Results:

Tables 113-115 summarize the observed phenotypes of transgenic plantsexpressing the genes constructs using the GH-SB Assays.

The genes listed in Tables 113-115 improved plant performance when grownat normal conditions. These genes produced larger plants with a largerphotosynthetic area (increased photosynthetic capacity), biomass (freshweight, dry weight, rosette diameter, rosette area and plot coverage),relative growth rate, blade relative area and petiole relative area. Thegenes were cloned under the regulation of a constitutive At6669 promoter(SEQ ID NO: 8190). The evaluation of each gene was performed by testingthe performance of different number of events. Event with p-value<0.1wasconsidered statistically significant.

TABLE 113 Genes showing improved plant performance at Normal growthconditions under regulation of At6669 promoter Dry Weight [mg] FreshWeight [mg] Leaf Number P- % P- % P- % Gene Name Event # Ave. Val. Incr.Ave. Val. Incr. Ave. Val. Incr. LYM1239 82025.4 — — — — — — 9.94 0.09  9LYM1239 82026.4 — — — 1962.5 0.01 21 — — — LYM1153 83267.4 175.0 0.22  51900.0 0.20 17 9.25 0.25  1 LYM1153 83267.5 — — — — — — 9.31 0.16  2LYM1153 83269.5 190.6 0.15 14 2037.5 L 25 — — — LYM1127 81669.2 — — — —— — 9.81 0.25  8 LYM1127 81671.6 178.1 0.23  7 1768.8 0.11  9 — — —LYM1125 83041.5 — — — — — — 9.38 0.23  3 LYM1125 83042.1 — — — 1856.20.03 14 9.25 0.25  1 LYM1087 81361.5 — — — 1743.8 0.18  7 — — — LYM107583047.2 176.2 0.11  6 1823.2 0.06 12 — — — LYM1075 83047.8 183.8 0.21 101875.0 0.03 15 — — — LYM1070 82533.4 199.0 0.07 19 1977.1 L 21 — — —LYM1044 82612.2 — — — 1711.6 0.24  5 — — — LYM1035 83262.1 — — — — — —9.44 0.04  3 CONT. — 166.8 — — 1628.6 — — 9.12 — — LYM1229 81574.1 331.2L 25 4062.5 L 23 — — — LYM1229 81575.3 333.8 0.02 26 3993.8 0.01 21 — —— LYM1229 81576.5 — — — — — — 9.94 0.04  9 LYM1221 81714.1 — — — 3612.50.27  9 — — — LYM1221 81714.5 — — — 3793.8 0.24 15 — — — LYM1221 81716.1— — — 3712.5 0.19 12 — — — LYM1217 80263.4 — — — 3518.8 0.26  6 — — —LYM1217 80264.2 312.4 0.09 18 3545.5 0.21  7 — — — LYM1212 80778.2 303.10.04 14 — — — — — — LYM1212 80779.2 297.3 0.10 12 3863.4 0.02 17 — — —LYM1212 80779.5 — — — 3670.5 0.17 11 — — — LYM1195 81917.3 350.8 L 324141.7 0.02 25 — — — LYM1195 81918.1 325.0 L 22 3887.5 0.02 17 — — —LYM1195 81919.3 298.8 0.12 13 3825.0 0.17 15 — — — LYM1194 81623.1 303.10.02 14 3762.5 0.03 14 — — — LYM1194 81624.2 300.0 0.05 13 3643.8 0.2910 — — — LYM1194 81624.6 287.6 0.20  8 — — — — — — LYM1146 81487.4 299.90.26 13 — — — — — — LYM1146 81488.2 323.8 L 22 4078.6 L 23 — — — LYM114681491.5 319.4 L 20 3868.8 0.03 17 — — — LYM1138 81477.5 300.0 0.21 133681.2 0.13 11 — — — LYM1138 81480.3 302.5 0.26 14 — — — — — — LYM113881480.4 303.1 0.04 14 3843.8 0.02 16 — — — LYM1129 81504.2 320.6 0.23 214062.5 0.21 23 — — — LYM1129 81505.2 286.9 0.13  8 376:2.5 0.10 14 — — —LYM1124 82008.2 287.5 0.26  8 — — — — — — LYM1124 82009.3 303.8 0.16 143875.0 0.23 17 9.62 0.05  5 LYM1124 82009.5 — — — 3750.0 0.04 13 — — —LYM1124 82010.2 288.8 0.15  9 — — — 9.50 0.16  4 LYM1111 81369.5 296.90.04 12 3643.8 0.14 10 9.44 0.18  3 LYM1111 81373.2 311.4 0.02 17 — — —— — — LYM1111 81373.4 285.3 0.25  7 3590.2 0.15  8 — — — LYM1111 81373.5302.5 0.05 14 3893.8 0.01 18 — — — LYM1087 81363.2 — — — 3606.2 0.12  9— — — LYM1087 81363.4 290.0 0.09  9 3793.8 0.03 15 — — — LYM1072 80972.1288.8 0.14  9 3537.5 0.23  7 — — — LYM1072 80973.2 320.0 0.03 21 3956.20.06 19 — — — LYM1072 80973.3 283.1 0.21 7 3568.8 0.17  8 — — — LYM107280975.2 296.2 0.14 12 3718.8 0.05 12 — — — LYM1053 80771.4 — — — 3693.80.10 12 — — — LYM1053 80772.3 — — — — — — 9.94 L  9 LYM1053 80772.5286.3 0.16  8 3775.9 0.03 14 — — — LYM1053 80774.3 303.1 0.03 14 3843.80.02 16 — — — LYM1042 82001.2 285.7 0.16  8 — — — — — — LYM1022 82057.2303.8 0.03 14 3731.2 0.06 13 — — — LYM1022 82057.4 310.0 0.29 17 3831.20.14 16 — — — LYM1022 82061.3 289.4 0.27  9 3612.5 0.27  9 — — — LYM101781072.2 300.5 0.03 13 3728.6 0.05 13 — — — LYM1017 81072.3 303.2 0.02 143708.0 0.11 12 — — — LYM1017 81074.1 311.2 L 17 3743.8 0.05 13 — — —CONT. — 265.4 — — 3312.2 — — 9.12 — — LYM1204 81052.2 — — — 3721.4 0.26 3 — — — LYM1151 80392.4 — — — 3712.5 0.25  3 — — — LYM1151 80392.6 — —— 3787.5 0.14  5 — — — LYM1151 80394.1 301.9 0.22  6 3775.0 0.07  4 — —— LYM1151 80394.2 300.0 0.23  5 3975.0 L 10 — — — LYM1151 80394.9 — — —3950.0 L  9 10.6 L 11 LYM1149 80387.5 310.0 0.08  9 3850.0 0.01  7 — — —LYM1149 80388.3 306.9 L  8 3818.8 0.05  6 — — — LYM1149 80388.5 — — —3700.0 0.26  2 — — — LYM1141 80271.2 — — — 3946.4 0.09  9 — — — LYM114180271.3 — — — 3756.2 0.23  4 10.0 0.09  5 LYM1141 80271.5 299.4 0.05  53856.2 0.01  7 9.94 0.10  5 LYM1141 80272.4 298.8 0.05  5 3993.8 0.04 11— — — LYM1139 80171.3 — — — — — — 10.2 0.24  8 LYM1139 80171.6 — — —3712.5 0.21  3 — — — LYM1139 80172.3 — — — 3833.9 0.23  6 — — — LYM113780169.1 309.4 0.08  9 3987.5 L 10 — — — LYM1133 81008.1 — — — 3737.50.14  3 — — — LYM1131 80191.2 305.4 0.01  7 3869.6 0.04  7 — — — LYM113180191.3 310.6 0.07  9 4106.2 L 14 — — — LYM1131 80193.3 303.1 0.03  63950.0 L  9 — — — LYM1117 80266.7 — — — 3800.0 0.16  5 — — — LYM111780268.6 — — — 3800.0 0.12  5 — — — LYM1103 80999.1 — — — 3937.5 0.17  9— — — LYM1103 81001.1 305.8 0.12  7 3893.8 0.16  8 — — — LYM1089 81093.1— — — 3737.5 0.20  3 — — — LYM1089 81096.4 305.6 0.01  7 3812.5 0.10  6— — — LYM1089 81096.6 — — — 3743.8 0.26  4 — — — LYM1069 81087.1 294.40.16  3 3850.0 0.02  7 — — — LYM1069 81091.2 — — — 3768.8 0.15  4 — — —LYM1047 81177.2 — — — 3750.0 0.10  4 — — — LYM1014 81067.1 — — — — — —10.2 0.11  8 LYM1014 81068.1 — _ — 3871.4 0.01  7 — — — LYM1014 81069.4292.5 0.23  3 3843.8 0.29  6 10.1 0.27  6 LYM1013 80311.1 — — — 3756.20.10  4 — — — LYM1013 80311.2 — — — 3950.0 0.30  9 — — — LYM1013 80313.1296.9 0.11  4 3743.8 0.11  4 — — — LYM1013 80315.3 — — — 3793.8 0.15  5— — — LYM1011 80942.2 — — — — — — 10.4 0.20  9 LYM1011 80945.2 — — — — —— 10.2 0.21  7 CONT. — 284.6 — — 3612.5 — — 9.50 — — LYM1236 80505.2 — —— — — — 10.2 0.23  4 LYM1236 80505.5 — — — 3787.5 0.03 20 — — — LYM123680506.5 279.4 0.11 11 — — — 10.4 0.02  7 LYM1227 81179.2 278.1 0.07 113862.5 L 23 — — — LYM1227 81179.7 305.6 0.16 22 4168.8 0.02 33 — — —LYM1213 81340.4 306.9 0.02 22 4243.8 0.07 35 — — — LYM1213 81343.3 — — —— — — 10.6 0.09  9 LYM1213 81343.4 333.8 L 33 4431.2 L 41 10.1 0.26  3LYM1213 81343.6 — — — — — — 10.3 0.05  5 LYM1203 81750.1 283.8 0.02 133725.0 L 18 10.8 0.11 10 LYM1203 81751.2 — — — — — — 10.1 0.16  3LYM1203 81751.5 — — — 3318.8 0.18  6 10.2 0.23  4 LYM1203 81752.6 — — —— — — 10.9 L 12 LYM1196 80558.4 281.2 0.04 12 3475.0 0.15 11 10.4 0.08 7 LYM1196 80561.4 — — — 3306.2 0.25  5 — — — LYM1183 81225.1 — — —3350.0 0.28  7 — — — LYM1183 81226.1 301.9 L 20 4175.0 L 33 — — —LYM1182 81492.1 — — — 3375.0 0.10  7 — — — LYM1182 81493.2 307.5 L 234050.0 0.12 29 — — — LYM1182 81494.2 271.7 0.11  8 3423.2 0.06  9 10.20.23  4 LYM1156 81017.1 273.8 0.19  9 3781.2 0.18 20 — — — LYM115681017.4 273.1 0.15  9 — — — 10.1 0.16  3 LYM1132 82013.2 — — — 3287.50.27  5 — — — LYM1127 81669.2 275.0 0.05 10 — — — — — — LYM1127 81669.4— — — 3331.2 0.27  6 — — — LYM1127 81671.6 308.1 L 23 4150.0 L 32 — — —LYM1094 81744.3 — — — — — — 10.4 0.08  7 LYM1094 81746.3 321.2 L 284150.0 L 32 — — — LYM1094 81747.2 280.6 0.03 12 3556.2 0.01 13 — — —LYM1093 81711.4 — — — 3287.5 0.26  5 — — — LYM1093 81711.6 — — — 341:2.50.12  9 — — — LYM1093 81713.4 289.4 0.18 15 3393.8 0.25  8 — — — LYM106080965.3 267.5 0.15  7 3412.5 0.08  9 — — — LYM1060 80965.6 — — — — — —10.2 0.11  5 LYM1060 80966.1 — — — — — — 10.8 0.11 10 LYM1057 81989.1264.4 0.23  5 3306.2 0.25  5 — — — LYM1051 80331.1 :276.9 0.20 10 — — —— — — LYM1051 80331.2 271.2 0.09  8 3437.5 0.05  9 — — — LYM1026 80322.5— — — 3312.5 0.21  5 — — — LYM1026 80323.2 263.8 0.27  5 3362.5 0.11  7— — — LYM1020 81482.3 — — — — — — 10.3 0.14  5 LYM1020 81483.1 295.00.26 18 4037.5 0.10 28 10.1 0.22  3 LYM1020 81484.3 312.5 L 25 3743.80.01 19 10.2 0.07  5 LYM1015 80090.5 296.9 L 18 3906.2 L 24 — — — CONT.— 251.0 — — 3144.4 — — 9.79 — — LYM1218 81116.6 — — — — — — 9.69 0.17  3LYM1218 81116.7 — — — — — — 9.69 0.17  3 LYM1218 81116.8 — — — — — —10.1 0.26  7 LYM1216 80260.2 438.8 0.01 19 5556.2 L 17 — — — LYM121080618.1 — — — 4925.0 0.09  4 — — — LYM1209 80480.1 — — — — — — 9.75 0.18 3 LYM1207 80247.5 — — — 4987.5 0.05  5 — — — LYM1207 80249.2 412.5 L 125137.5 L  8 — — — LYM1207 80250.1 — — — 4937.5 0.08  4 9.62 0.26  2LYM1201 81104.1 388.1 0.19  5 5187.5 L  9 — — — LYM1192 81128.3 385.00.19  4 5087.5 0.02  7 — — — LYM1191 80380.1 381.9 0.29  3 5143.8 L  8 —— — LYM1191 80383.1 — — — 5100.0 L  7 — — — LYM1189 81099.1 386.9 0.15 5 5043.8 0.13  6 — — — LYM1176 80810.3 — — — 5087.5 0.04  7 — — —LYM1176 80811.3 — — — 5016.1 0.05  6 — — — LYM1173 80465.1 — — — 5081.20.14  7 — — — LYM1170 80187.4 — — — — — — 9.69 0.17  3 LYM1170 80188.3420.6 L 14 5606.2 L 18 9.81 0.05  4 LYM1169 80606.2 — — — 4875.0 0.20  3— — — LYM1169 80610.3 — — — — — — 9.94 0.02  5 LYM1169 80610.4 — — —4914.3 0.13  4 — — — LYM1161 80178.4 — — — 4906.2 0.21  3 — — — LYM115880461.3 — — — 5031.2 0.14  6 — — — LYM1115 80134.2 — — — 5118.8 0.06  8— — — LYM1115 80135.2 — — — 5218.8 L 10 — — — LYM1073 80981.1 — — —4943.8 0.07  4 — — — LYM1054 80109.1 — — — — — — 9.81 0.05  4 LYM105480110.4 423.1 L 15 5412.5 L 14 — — — CONT. — 369.5 — — 4746.4 — — 9.45 —— LYM1223 81211.3 359.7 0.08  4 5335.7 0.02 16 — — — LYM1223 81212.3 — —— — — — 9.75 0.11  4 LYM1220 80484.2 374.6 L  8 4892.9 0.14  6 — — —LYM1220 80484.5 376.2 0.07  9 — — — 9.56 0.25  2 LYM1220 80484.6 — — — —— — 9.62 0.25  3 LYM1220 80486.1 — — — — — — 9.75 0.11  4 LYM121180254.3 — — — 4787.5 0.27  4 — — — LYM1211 80255.2 355.6 0.18  3 — — — —— — LYM1202 81107.2 — — — — — — 10.0 0.03  7 LYM1202 81107.3 — — —5175.0 L 13 — — — LYM1111 81369.4 — — — 4954.2 0.14  8 9.62 0.12  3LYM1111 81369.5 — — — 5042.0 0.15 10 — — — LYM1111 81373.2 357.5 0.03  45093.8 0.01 11 — — — LYM1111 81373.4 368.1 L  7 5187.5 0.02 13 — — —LYM1111 81373.6 365.6 0.10  6 — — — — — — LYM1108 80120.3 352.5 0.15  2— — — — — — LYM1072 80975.5 — — — — — — 9.69 0.30  3 LYM1063 81348.4 — —— 5050.0 0.02 10 — — — LYM1053 80771.4 — — — — — — 9.81 0.02  5 LYM105380774.3 — — — 5012.5 0.03  9 — — — LYM1051 80331.3 — — — — — — 9.94 0.12LYM1029 81353.6 — — — — — — 9.56 0.25  2 LYM1026 80323.2 356.9 0.01  3 —— — — — — LYM1021 81204.4 387.4 0.14 12 4930.4 0.07  7 9.69 0.30  3LYM1017 81072.2 — — — — — — 10.2 0.11  9 LYM1017 81072.3 375.6 L  95450.0 L 19 — — — LYM1017 81072.4 357.5 0.26  4 4912.5 0.25  7 — — —LYM1016 80947.2 — — — 4891.1 0.28  6 — — — LYM1016 80948.2 368.8 L  74818.8 0.27  5 — — — LYM1016 80950.3 360.6 L  4 5075.0 0.28 10 — — —LYM1015 80088.6 361.1 0.18  5 — — — — — — LYM1015 80089.5 370.0 0.14  7— — — — — — CONT — 345.4 — — 4599.0 — — 9.38 — — LYM1187 82688.7 175.60.29 23 — — — — — — LYM1187 82689.5 — — — 1568.8 0.30 13 — — — LYM118482873.5 — — — 1706.2 0.17 23 — — — LYM1154 82551.2 173.8 0.25 22 — — —9.62 0.23  5 LYM1154 82553.6 — — — 1681.2 0.06 22 — — — LYM1154 82553.9177.5 0.03 24 2056.2 0.04 49 10.1 0.24 10 LYM1142 83549.1 162.5 0.16 141575.0 0.27 14 — — — LYM1123 82866.2 167.5 0.12 17 — — — — — — LYM112182541.3 164.4 0.13 15 1793.8 0.10 30 — — — LYM1114 82487.11 — — — 1725.00.04 25 — — — LYM1106 82625.4 — — — 1680.4 0.06 21 — — — LYM1105_H282620.2 161.9 0.22 13 1831.2 0.02 32 — — — LYM1105_H2 82621.1 — — —1568.8 0.20 13 — — — LYM1101_H3 82615.10 158.1 0.29 11 — — — — — —LYM1086 82371.8 169.4 0.08 19 1775.0 0.02 28 — — — LYM1086 82373.3 183.40.22 28 1782.1 0.03 29 — — — LYM1078 82657.5 182.5 0.02 28 1918.8 0.0439 — — — LYM1078 82659.5 167.0 0.11 17 — — — — — — LYM1074 82571.4 161.20.19 13 1600.0 0.18 16 — — — LYM1074 82572.2 — — — 1662.5 0.08 20 — — —LYM1058_H4 83372.5 173.4 0.13 21 1817.0 0.12 31 — — — LYM1058_H4 83372.6184.4 0.06 29 1837.5 0.01 33 — — — LYM1058_H4 83374.1 158.9 0.28 11 — —— — — — LYM1037 82524.2 — — — 1642.0 0.24 19 — — — LYM1037 82524.3 — — —1725.0 0.24 25 — — — LYM1033 82883.4 163.1 0.25 14 1812.5 0.03 31 — — —LYM1033 82885.5 — — — 1749.1 0.04 26 9.56 0.06  5 LYM1023 82516.1 178.10.06 25 1856.2 0.01 34 — — — CONT. — 142.8 — — 1383.2 — — 9.12 — —LYM1235 81117.1 — — — 4106.2 0.10  7 — — — LYM1235 81121.1 308.8 0.29  5— — — — — — LYM1235 81121.3 — — — 3987.5 0.17  4 — — — LYM1233 80500.2310.0 0.02  6 — — — — — — LYM1233 80500.3 310.6 0.06  6 4200.0 0.01  9 —— — LYM1233 80501.2 333.1 0.03 14 4437.5 L 15 — — — LYM1233 80503.4 — —— 4125.0 0.20  7 — — — LYM1231 80494.2 351.2 0.15 20 4400.0 0.06 14 — —— LYM1231 80496.6 323.1 0.01 10 4068.7 0.22  6 — — — LYM1230 80490.1315.0 0.27  7 4100.0 0.03  7 — — — LYM1230 80490.2 — — — 4000.0 0.25  4— — — LYM1230 80493.5 — — — — — — 9.75 0.10  3 LYM1223 81210.3 306.20.06  4 — — — — — — LYM1223 81211.3 314.4 0.03  7 4031.2 0.16  5 — — —LYM1223 81212.3 322.5 0.05 10 4150.0 0.02  8 — — — LYM1220 80484.2 324.40.19 11 4100.0 0.23  7 — — — LYM1220 80484.3 — — — 4181.2 0.05  9 — — —LYM1220 80484.5 333.1 L 14 4125.0 0.02  7 — — — LYM1220 80486.1 338.8 L15 4243.8 L 10 — — — LYM1219 80510.2 325.6 L 11 4200.0 L  9 — — —LYM1219 80510.3 320.0 L  9 4250.0 L 10 — — — LYM1219 80513.1 308.2 0.05 5 4075.0 0.26  6 — — — LYM1219 80513.3 — — — 4031.2 0.16  5 — — —LYM1211 80254.3 308.8 0.23  5 — — — — — — LYM1211 80255.2 304.4 0.24  44081.2 0.07  6 — — — LYM1202 81107.2 303.1 0.21  3 — — — — — — LYM120281108.1 330.0 L 12 4131.2 0.02  7 — — — LYM1202 81109.2 310.0 0.26  63993.8 0.21  4 — — — LYM1202 81109.3 — — — — — — 9.62 0.30  2 LYM110980123.1 315.6 L  8 4231.2 L 10 — — — LYM1109 80123.2 300.6 0.27  23968.8 0.26  3 — — — LYM1109 80125.2 309.4 0.03  5 4050.0 0.06  5 — — —LYM1108 80116.1 — — — 4175.0 0.07  9 — — — LYM1108 80119.3 301.9 0.20  3— — — — — — LYM1102 80994.1 325.6 L 11 4137.5 0.04  8 — — — LYM109780983.1 — — — 4125.0 0.20  7 — — — LYM1063 81347.1 328.1 0.22 12 4287.50.21 11 — — — LYM1063 81348.2 — — — 4112.5 0.02  7 — — — LYM1021 81204.4309.4 0.02  5 4037.5 0.17  5 — — — LYM1021 81208.2 314.4 0.01  7 3993.80.15  4 — — — LYM1016 80948.2 — — — — — — 9.88 0.29  5 LYM1016 80950.2315.0 L  7 4037.5 0.21  5 — — — LYM1010 81062.1 313.1 0.02  7 4081.20.07  6 — — — LYM1010 81062.2 — — — 4000.0 0.14  4 — — — LYM1010 81063.4326.9 0.02 11 4068.7 0.08  6 — — — LYM1010 81064.1 305.0 0.19  4 — — — —— — CONT. — 293.4 — — 3846.4 — — 9.45 — — LYM1107 82649.3 — — — 1692.90.39  4 — — — CONT. — — — — 1628.6 — — — — — “CONT.” - Control; “Ave.” -Average; “% Incr.” = % increment; “p-val.” - p-value, L- p <0.01.

TABLE 114 Genes showing improved plant performance at Normal growthconditions under regulation of At6669 promoter Plot Coverage RosetteDiameter [cm²] Rosette Area [cm²] [cm] P. % P- % P- % Gene Name Event #Ave. Val. Incr. Ave. Val. Incr. Ave. Val Incr. LYM1239 82026.4 53.3 0.2121 6.67 0.21 21 4.46 0.10 LYM1153 83265.5 48.0 0.04  9 5.99 0.04  9 — —— LYM1153 83269.5 55.4 L 26 6.92 L 26 4.49 L  9 LYM1129 81505.5 46.70.11  6 5.84 0.11  6 — — — LYM1127 81671.6 45.9 0.23  5 5.74 0.23  5 — —— LYM1125 83042.1 47.6 0.05  8 5.95 0.05  8 4.27 0.28  3 LYM1125 83042.347.6 0.06  8 5.95 0.06  8 4.30 0.23  4 LYM1125 83042.5 49.5 L 13 6.18 L13 4.36 0.02 — LYM1097 80984.1 — — — — — — 4.25 0.15  3 LYM1093 81711.2— — — — — — 4.32 0.23  4 LYM1093 81713.4 48.5 0.13 10 6.06 0.13 10 — — —LYM1088 83558.5 45.9 0.26  5 5.74 0.26  5 — — — LYM1085 82683.2 — — — —— — 4.24 0.18  3 LYM1075 83047.8 47.7 0.17  9 5.96 0.17  9 — — — LYM107583049.1 45.9 0.24  5 5.74 0.24  5 4.26 0.13  3 LYM1070 82533.4 48.5 0.0211 6.07 0.02 11 4.26 0.13  3 LYM1035 83264.6 47.1 0.10  7 5.88 0.10  7 —— — CONT. — 43.9 — — 5.49 — — 4.13 — — LYM1229 81574.1 60.3 0.04 28 7.540.04 28 4.59 0.04 13 LYM1229 81575.3 63.4 0.03 34 7.92 0.03 34 4.72 0.0216 LYM1229 81576.5 59.4 0.03 26 7.43 0.03 26 4.69 0.02 15 LYM122181714.4 54.9 0.14 16 6.86 0.14 16 4.59 0.04 13 LYM1221 81716.1 52.9 0.2512 6.61 0.25 12 4.36 0.19  7 LYM1221 81718.1 — — — — — — 4.30 0.29  6LYM1212 80778.2 — — — — — — 4.34 0.22  7 LYM1195 81918.1 62.1 0.02 327.76 0.02 32 4.68 0.02 15 LYM1195 81919.3 53.8 0.19 14 6.73 0.19 14 4.570.05 12 LYM1195 81919.6 — — — — — — 4.39 0.15  8 LYM1194 81623.1 55.60.21 18 6.95 0.21 18 4.52 0.22 11 LYM1194 81624.2 53.0 0.24 12 6.63 0.2412 4.52 0.06 11 LYM1194 81624.4 — — — — — — 4.31 0.30  6 LYM1175 82018.253.3 0.22 13 6.67 0.22 13 4.35 0.21  7 LYM1146 81488.2 54.6 0.15 16 6.830.15 16 4.59 0.04 13 LYM1138 81477.5 — — — — — — 4.48 0.11 10 LYM113881480.1 — — — — — — 4.56 0.23 12 LYM1129 81504.2 59.3 0.21 26 7.41 0.2126 4.80 0.01  1 LYM1124 82008.2 56.5 0.23 20 7.06 0.23 20 4.54 0.14 11LYM1124 82009.3 60.9 0.02 29 7.62 0.02 29 4.76 0.01 17 LYM1124 82009.553.4 0.23 13 6.68 0.23 13 4.40 0.15  8 LYM1124 82010.2 58.4 0.05 24 7.300.05 24 4.62 0.04 14 LYM1111 81369.5 — — — — — — 4.44 0.27  9 LYM111181373.5 55.1 0.18 17 6.89 0.18 17 4.44 0.12  9 LYM1072 80973.2 — — — — —— 4.38 0.16  8 LYM1053 80772.3 57.4 0.06 22 7.18 0.06 22 4.55 0.05 12LYM1053 80772.5 — — — — — — 4.40 0.18  8 LYM1042 82001.2 — — — — — —4.49 0.22 10 LYM1022 82057.2 — — — — — — 4.42 0.13  9 LYM1022 82057.458.6 0.05 24 7.32 0.05 24 4.64 0.03 14 LYM1022 82061.3 58.7 0.17 24 7.340.17 24 4.65 0.18 14 LYM1017 81072.3 53.6 0.20 14 6.70 0.20 14 4.48 0.0810 CONT. — 47.2 — — 5.90 — — 4.07 — — LYM1204 81054.3 — — — — — — 4.560.23  3 LYM1151 80394.1 53.4 0.14  8 6.67 0.14  8 4.58 0.14  4 LYM115180394.9 — — — — — — 4.55 0.23  3 LYM1141 80271.3 54.1 0.14 10 6.76 0.1410 4.60 0.24  4 LYM1141 80271.5 60.9 0.08 24 7.61 0.08 24 4.81 0.17  9LYM1139 80171.3 64.3 L 31 8.04 L 31 5.12 L 16 LYM1117 80266.7 53.0 0.12 8 6.62 0.12  8 4.65 0.11  6 LYM1069 81087.1 57.2 L 16 7.14 L 16 4.830.19 10 LYM1011 80942.2 57.9 L 18 7.24 L 18 4.77 0.02  8 LYM1011 80945.253.9 0.28  9 6.74 0.28  9 — — — CONT. — 49.3 — — 6.16 — — 4.40 — —LYM1236 80506.5 75.4 0.06 22 9.43 0.06 22 5.13 0.24 10 LYM1227 81179.572.4 0.11 17 9.04 0.11 17 5.19 0.04 11 LYM1213 81343.4 73.3 0.08 18 9.160.08 18 5.14 0.07 10 LYM1213 81343.6 71.9 0.24 16 8.99 0.24 16 5.06 0.18 8 LYM1203 81750.1 76.0 0.04 23 9.50 0.04 23 5.11 0.08  9 LYM120381751.2 71.2 0.25 15 8.90 0.25 15 — — — LYM1203 81751.5 78.5 0.02 279.81 0.02 27 5.26 0.03 12 LYM1203 81752.6 71.0 0.15 15 8.88 0.15 15 5.050.14  8 LYM1196 80558.4 79.8 0.02 29 9.98 0.02 29 5.33 0.02 14 LYM119680558.5 68.2 0.29 10 8.52 0.29 10 4.96 0.23  6 LYM1182 81494.2 71.3 0.1315 8.92 0.13 15 5.08 0.09  8 LYM1156 81017.4 70.6 0.24 14 8.82 0.24 145.00 0.17  7 LYM1127 81669.2 74.5 0.06 20 9.32 0.06 20 5.22 0.03 11LYM1094 81744.3 72.1 0.11 16 9.01 0.11 16 5.02 0.13  7 LYM1094 81746.368.2 0.30 10 8.53 0.30 10 4.96 0.22  6 LYM1093 81713.4 70.4 0.25 14 8.800.25 14 — — — LYM1060 80965.3 70.7 0.16 14 8.84 0.16 14 5.00 0.16  7LYM1060 80965.4 79.2 0.08 28 9.90 0.08 28 5.30 0.11 13 LYM1060 80965.674.2 0.06 20 9.27 0.06 20 5.12 0.07  9 LYM1057 81989.1 — — — — — — 4.980.18  6 LYM1051 80331.2 68.9 0.24 11 8.61 0.24 11 — — — LYM1026 80325.373.7 0.15 19 9.21 0.15 19 5.04 0.15  8 LYM1020 81482.3 73.2 0.11 18 9.150.11 18 5.11 0.08  9 LYM1020 81483.1 — — — — — — 4.93 0.27  5 LYM102081484.3 74.0 0.07 19 9.25 0.07 19 5.26 0.03 12 LYM1015 80089.4 72.8 0.1717 9.09 0.17 17 — — — CONT. — 62.0 — — 7.75 — — 4.68 — — LYM1218 81116.637.9 0.02 14 4.74 0.02 14 3.98 0.02  9 LYM1207 80247.4 35.1 0.17  6 4.390.17  6 3.83 0.07  5 LYM1207 80249.2 44.2 0.19 33 5.52 0.19 33 4.33 0.1718 LYM1201 81104.1 39.7 L 20 4.96 L 20 3.99 0.15  9 LYM1201 81104.4 36.80.13 11 4.60 0.13 11 3.90 0.02  7 LYM1191 80380.1 35.3 0.20  6 4.41 0.20 6 3.83 0.08  5 LYM1191 80381.4 39.5 L 19 4.93 L 19 3.91 0.06  7 LYM118981099.1 40.6 0.08 22 5.08 0.08 22 4.08 L 12 LYM1176 80810.3 37.5 0.03 134.69 0.03 13 3.91 0.06  7 LYM1176 80811.3 34.7 0.27  4 4.33 0.27  4 — —— LYM1173 80465.1 36.1 0.06  9 4.52 0.06  9 3.87 0.04  6 LYM1170 80188.337.4 0.20 13 4.68 0.20 13 3.93 0.04  7 LYM1161 80179.1 47.9 0.04 44 5.990.04 44 4.34 0.03 19 LYM1158 80461.5 36.2 0.04  9 4.53 0.04  9 3.94 0.13 8 LYM1115 80135.2 — — — — — — 3.85 0.12  5 LYM1054 80110.4 43.3 0.01 315.41 0.01 31 4.26 L 16 CONT. — 33.2 — — 4.15 — — 3.66 — — LYM122080484.2 45.6 0.30 15 5.71 0.30 15 4.39 0.13  8 LYM1220 80484.3 48.4 0.1222 6.05 0.12 22 4.51 0.15 11 LYM1220 80484.5 50.6 0.09 27 6.32 0.09 274.56 0.21 12 LYM1220 80486.1 55.1 0.28 39 6.89 0.28 39 — — — LYM121180255.2 43.9 0.21 10 5.49 0.21 10 4.32 0.15  6 LYM1202 81107.3 43.1 0.30 8 5.39 0.30  8 4.22 0.22  4 LYM1111 81373.4 44.2 0.08 11 5.53 0.08 114.35 L  7 LYM1111 81373.6 48.9 0.19 23 6.12 0.19 23 4.56 0.22 12 LYM110880120.3 43.6 0.27 10 5.45 0.27 10 4.32 0.06  6 LYM1053 80772.5 41.8 0.16 5 5.22 0.16  5 — — — LYM1051 80331.1 43.3 0.09  9 5.42 0.09  9 4.40 L 8 LYM1021 81204.4 42.0 0.22  6 5.25 0.22  6 4.25 0.03  5 LYM101781072.2 51.4 L 29 6.43 L 29 4.62 L 14 LYM1017 81072.3 49.4 L 24 6.18 L24 4.71 L 16 LYM1017 81076.2 — — — — — — 4.35 0.24  7 LYM1016 80947.241.8 0.07  5 5.22 0.07  5 4.22 0.05  4 LYM1016 80948.2 46.0 L 16 5.76 L16 4.36 0.15  7 LYM1016 80948.3 43.4 0.26  9 5.42 0.26  9 4.21 0.26  3LYM1016 80950.3 45.1 0.14 13 5.63 0.14 13 4.35 L  7 LYM1015 80089.5 46.40.13 17 5.80 0.13 17 4.36 0.02  7 CONT. — 39.8 — — 4.97 — — 4.07 — —LYM1187 82688.7 47.8 0.12 17 5.98 0.13 14 4.20 0.10  8 LYM1187 82688.9 —— — — — — 4.45 0.22 14 LYM1184 82873.5 — — — — — — 4.29 0.12 10 LYM115482551.2 57.7 0.01 41 7.21 0.02 37 4.61 L 19 LYM1154 82553.9 62.9 0.06 547.87 0.08 50 4.78 0.04 23 LYM1142 83549.1 47.2 0.14 16 5.90 0.15 12 4.090.23  5 LYM1121 82541.3 55.0 0.09 35 6.88 0.12 31 4.55 0.08 17 LYM111482487.11 48.3 0.13 18 6.03 0.14 15 4.38 0.04 13 LYM1106 82625.4 47.50.13 16 5.94 0.12 13 4.22 0.07  9 LYM1105_H2 82620.2 53.5 0.01 31 6.69 L27 4.44 L 14 LYM1086 82371.8 51.6 0.03 26 6.45 0.02 23 4.44 0.01 14LYM1086 82373.3 51.0 0.08 25 6.37 0.09 21 4.26 0.18 10 LYM1078 82657.556.5 0.06 38 7.06 0.08 34 4.51 0.12 16 LYM1078 82659.2 46.0 0.22 13 5.750.24  9 — — — LYM1074 82570.1 46.0 0.26 13 — — — 4.12 0.20  6 LYM107482570.2 46.7 0.17 14 5.83 0.19 11 4.07 0.27  5 LYM1074 82571.4 — — — — —— 4.15 0.26  7 LYM1074 82572.2 49.2 0.07 20 6.15 0.07 17 4.34 0.02 11LYM1058_H4 83372.6 52.3 0.02 28 6.53 0.02 24 4.42 L 14 LYM1037 82524.347.3 0.27 16 — — — — — — LYM1033 82883.4 54.7 0.03 34 6.84 0.04 30 4.560.05 17 LYM1023 82516.1 56.7 L 39 7.09 L 35 4.57 L 17 CONT. — 40.8 — —5.26 — — 3.89 — — LYM1235 81121.1 30.3 0.20 14 3.79 0.20 14 — — —LYM1235 81121.3 27.8 0.28  5 3.47 0.28  5 — — — LYM1233 80500.2 31.00.03 17 3.88 0.03 17 3.70 L 12 LYM1233 80501.2 29.5 0.03 11 3.69 0.03 113.43 0.04  4 LYM1233 80502.5 28.8 0.17  9 3.60 0.17  9 — — — LYM123380503.4 35.3 0.16 33 4.41 0.16 33 3.79 0.14 15 LYM1231 80494.2 31.9 L 203.99 L 20 3.67 L 11 LYM1231 80496.2 33.4 L 26 4.17 L 26 3.77 0.19 14LYM1231 80496.6 31.1 L 17 3.88 L 17 3.55 0.05  8 LYM1230 80493.5 30.10.18 14 3.76 0.18 14 3.62 0.09 10 LYM1220 80484.2 28.9 0.07  9 3.62 0.07 9 — — — LYM1220 80484.3 30.4 0.08 15 3.80 0.08 15 3.48 0.25 — LYM122080484.5 30.6 0.06 16 3.83 0.06 16 3.53 L  7 LYM1220 80484.6 — — — — — —3.42 0.04  4 LYM1220 80486.1 39.4 0.25 49 4.93 0.25 49 4.00 0.23 21LYM1211 80255.2 — — — — — — 3.40 0.13  3 LYM1202 81107.2 30.5 0.13 153.81 0.13 15 3.50 0.05  6 LYM1202 81108.1 32.9 0.01 24 4.11 0.01 24 3.67L 11 LYM1202 81109.2 29.8 0.02 13 3.73 0.0:2 13 3.55 L  8 LYM120281109.3 28.4 0.15  7 3.55 0.15  7 3.38 0.15  2 LYM1109 80123.1 33.7 L 274.21 L 27 3.80 0.09 15 LYM1108 80120.1 — — — — — — 3.38 0.28  2 LYM110880120.3 28.9 0.27  9 3.62 0.27  9 3.54 0.25  7 LYM1063 81347.1 30.2 0.2314 3.77 0.23 14 — — — LYM1021 81204.4 28.6 0.10  8 3.58 0.10  8 3.47 L 5 LYM1016 80948.2 29.2 0.05 10 3.65 0.05 10 3.44 0.03  4 LYM101680950.3 28.3 015  7 3.54 0.15  7 3.43 0.10  4 LYM1010 81062.1 30.5 0.0215 3.82 0.02 15 3.43 0.04  4 CONT. — 26.5 — — 3.31 — — 3.30 — —“CONT.” - Control; “Ave.” - Average “% Incr.” = % increment; “p-val.” -p-value, L- p <0.01.

TABLE 115 Genes showing proved plant performance at Normal growthconditions under regulation of At666.9 promoter RGR Of Leaf RGR Of PlotRGR Of Rosette Number Coverage % Diameter P- % P- % P- % Gene Name Event# Ave. Val. Incr. Ave. Val. Incr. Ave. Val. Incr. LYM1239 82025.4 0.6270.21 27 — — — — — — LYM1239 82026.4 — — — 6.74 0.17 24 0.391 0.28 11LYM1153 83267.2 — — — 6.69 0.20 23 — — — LYM1153 83267.4 — — — 6.63 0.2122 — — — LYM1153 83267.5 0.619 0.21 25 — — — — — — LYM1153 83269.5 — — —6.93 0.12 27 — — — LYM1127 81669.4 — — — 6.51 0.27 19 — — — CONT. —0.495 — — 5.46 — — 0.353 — — LYM1229 81574.1 — — — 7.55 0.20 26 — — —LYM1229 81575.3 — — — 7.90 0.12 32 — — — LYM1229 81576.5 — — — 7.41 0.2324 — — — LYM1195 81918.1 — — — 7.81 0.14 31 — — — LYM1129 81504.2 — — —7.48 0.22 25 — — — LYM1124 82009.3 — — — 7.72 0.16 29 0.417 0.25 18LYM1124 82010.2 — — — 7.34 0.26 23 — — — LYM1022 82057.4 — — — 7.43 0.2324 — — — LYM1022 82061.3 — — — 7.36 0.26 23 — — — CONT. — — — — 5.98 — —0.353 — — LYM1151 80394.9 0.650 0.07 22 — — — — — — LYM1141 80271.5 — —— 5.99 0.12 21 — — — LYM1139 80171.3 — — — 6.39 0.03 30 0.394 0.03 16LYM1069 81087.1 — — — 5.73 0.21 16 0.375 0.16 10 LYM1011 80942.2 — — —5.84 0.16 18 0.371 0.22  9 CONT. — 0.535 — — 4.93 — — 0.340 — — LYM123680506.5 — — — 9.65 0.27 21 — — — LYM1203 81750.1 — — — 9.64 0.27 21 — —— LYM1203 81751.5 — — — 10.0 0.18 26 — — — LYM1196 80558.4 — — — 10.20.14 29 — — — LYM1127 81670.2 0.767 0.25 20 — — — — — — LYM1060 80965.30.752 0.30 18 — — — — — — LYM1060 80965.4 — — — 10.3 0.14 29 — — — CONT.— 0.638 — — 7.96 — — — — — LYM1218 81116.6 — — — 4.77 0.27 14 — — —LYM1216 80260.2 — — — 4.83 0.27 16 — — — LYM1209 80480.1 0.678 0.30 17 —— — — — — LYM1207 80247.4 — — — — — — 0.348 0.27  9 LYM1207 80249.2 — —— 5.53 0.02 32 0.372 0.06 16 LYM1201 81104.1 — — — 5.00 0.14 20 — — —LYM1191 80381.4 — — — 4.93 0.17 18 — — — LYM1189 81099.1 — — — 5.08 0.1022 — — — LYM1170 80188.3 — — — — — — 0.346 0.28  8 LYM1169 80610.3 0.7010.20 21 — — — — — — LYM1161 80179.1 — — — 6.00 L 44 0.362 0.09 13LYM1054 80110.4 — — — 5.41 0.03 30 0.362 0.10 13 CONT. — 0.579 — — 4.17— — 0.320 — — LYM1220 80484.2 — — — 5.77 0.19 16 0.390 0.09 12 LYM122080484.3 — — — 6.05 0.07 22 0.384 0.15 10 LYM1220 80484.5 — — — 6.39 0.0329 0.404 0.03 16 LYM1220 80486.1 — — — 6.84 L 38 0.388 0.23 12 LYM111181369.4 0.630 0.24 17 — — — — — — LYM1111 81373.6 — — — 6.14 0.06 230.401 0.05 15 LYM1108 80120.3 — — — — — — 0.385 0.14 11 LYM1102 80993.40.636 0.27 18 — — — — — — LYM1053 80771.4 0.642 0.21 19 — — — — — —LYM1053 80774.3 — — — — — — 0.378 0.24  9 LYM1051 80331.1 — — — — — —0.375 0.28  8 LYM1021 81207.3 0.638 0.25 19 — — — — — — LYM1017 81072.2— — — 6.50 0.01 31 0.398 0.04 14 LYM1017 81072.3 — — — 6.17 0.05 240.410 0.01 18 LYM1017 81076.2 — — — 5.70 0.24 15 0.382 0.17 10 LYM101680948.2 — — — 5.75 0.19 16 — — — LYM1016 80950.3 — — — 5.68 0.23 140.376 0.25  8 LYM1015 80089.4 — — — 5.77 0.19 16 0.380 0.24  9 LYM101580089.5 — — — 5.85 0.15 18 0.397 0.05 14 CONT. — 0.537 — — 4.97 — —0.348 — — LYM1187 82688.9 — — — 6.26 0.28 24 0.397 0.07 28 LYM118482873.5 — — — 6.29 0.26 24 0.388 0.09 25 LYM1154 82551.2 — — — 7.32 0.0545 0.400 0.05 29 LYM1154 82553.9 0.625 0.27 23 7.91 0.02 57 0.412 0.0333 LYM1123 82866.2 — — — 6.75 0.14 34 — — — LYM1121 82541.3 — — — 6.940.09 37 0.394 0.08 27 LYM1118 82534.7 — — — 6.94 0.10 37 0.379 0.15 22LYM1114 82487.11 — — — — — — 0.383 0.12 23 LYM1106 82625.4 — — — — — —0.380 0.12 22 LYM1105_H2 82620.2 — — — 6.79 0.12 34 0.395 0.07 27LYM1101_H3 82615.10 — — — — — — 0.376 0.17 21 LYM1101_H3 82615.6 — — — —— — 0.371 0.19 19 LYM1086 82371.6 — — — — — — 0.372 0.19 20 LYM108682371.8 — — — 6.49 0.19 28 0.384 0.10 24 LYM1086 82373.3 — — — 6.27 0.2624 — — — LYM1078 82657.5 — — — 7.09 0.07 40 0.375 0.15 21 LYM107882657.9 0.634 0.27 25 — — — — — — LYM1074 82571.4 — — — — — — 0.360 0.2716 LYM1074 82572.2 — — — 6.31 0.24 25 0.396 0.06 27 LYM1058_H4 83372.5 —— — 6.32 0.28 25 — — — LYM1058_H4 83372.6 — — — 6.50 0.18 29 0.367 0.2118 LYM1037 82524.3 — — — — — — 0.363 0.26 17 LYM1033 82883.4 — — — 6.920.10 37 0.393 0.07 27 LYM1033 82885.5 — — — — — — 0.363 0.26 17 LYM102382516.1 — — — 7.11 0.07 41 0.388 0.09 25 CONT. — 0.507 — — 5.05 — —0.311 — — LYM1235 81121.1 — — — 3.79 0.20 17 — — — LYM1233 80500.2 — — —3.82 0.19 18 0.314 0.05 11 LYM1233 80501.2 — — — — — — 0.308 0.12  9LYM1233 80503.4 — — — 4.34 0.02 34 0.326 0.02 15 LYM1231 80494.2 — — —3.94 0.10 22 0.309 0.08  9 LYM1231 80496.2 — — — 4.12 0.04 27 0.312 0.1010 LYM1231 80496.6 — — — 3.81 0.19 17 0.303 0.22  7 LYM1230 80493.5 — —— — — — 0.300 0.30  6 LYM1223 81211.4 — — — — — — 0.301 0.27  6 LYM122080484.3 — — — 3.69 0.29 14 — — — LYM1220 80484.5 — — — 3.80 0.19 170.313 0.05 11 LYM1220 80486.1 — — — 4.82 L 49 0.326 0.04 15 LYM120281107.2 — — — 3.72 0.26 15 — — — LYM1202 81108.1 — — — 4.07 0.06 260.317 0.04 12 LYM1202 81109.2 — — — 3.70 0.29 14 0.313 0.07 10 LYM110980123.1 — — — 4.11 0.05 27 0.322 0.02 14 LYM1109 80125.7 — — — — — —0.301 0.28  6 LYM1108 80120.3 — — — — — — 0.302 0.23 7 LYM1063 81347.1 —— — 3.71 0.28 14 — — — LYM1021 81204.4 — — — — — — 0.309 0.10  9 LYM101680950.3 — — — — — — 0.299 0.30  5 LYM1010 81062.1 — — — 3.79 0.20 17 — —— LYM1010 81063.4 — — — 4.05 0.09 25 0.316 0.08 11 CONT. — — — — 3.24 —— 0.284 — — “CONT.” - Control; “Ave.” - Average “% Incr.” = % increment;“p-val.” - p-value, L- p <0.01.

Example 21 Evaluating Transgenic Arabidopsis Under Normal ConditionsUsing Seedling Analysis [TC-T2 and TC-T1 Assays]

Surface sterilized seeds were sown in basal media [50% Murashige-Skoogmedium (MS) supplemented with 0.8% plant agar as solidifying agent] inthe presence of Kanamycin (used as a selecting agent). After sowing,plates were transferred for 2-3 days for stratification at 4° C. andthen grown at 25° C. under 12-hour light 12-hour dark daily cycles for 7to 10 days. At this time point, seedlings randomly chosen were carefullytransferred to plates containing ½ MS media (15 mM N). For experimentsperformed in T₂lines, each plate contained 5seedlings of the sametransgenic event, and 3-4 different plates (replicates) for each event.For each polynucleotide of the invention at least four-five independenttransformation events were analyzed from each construct. For experimentsperformed in T₁ lines, each plate contained 5 seedlings of 5 independenttransgenic events and 3-4 different plates (replicates) were planted. Intotal, for T₁ lines, 20 independent events were evaluated. Plantsexpressing the polynucleotides of the invention were compared to theaverage measurement of the control plants (empty vector or GUS reportergene under the same promoter) used in the same experiment.

Digital imaging—A laboratory image acquisition system, which consists ofa digital reflex camera (Canon EOS 300D) attached with a 55 mm focallength lens (Canon EF-S series), mounted on a reproduction device(Kaiser RS), which includes 4 light units (4×150 Watts light bulb) andlocated in a darkroom, was used for capturing images of plantlets sawnin agar plates.

The image capturing process was repeated every 3-4 days starting at day1 till day 10 (see for example the images in FIGS. 3A-3F). An imageanalysis system was used, which consists of a personal desktop computer(Intel P4 3.0 GHz processor) and a public domain program—ImageJ 1.39[Java based image processing program which was developed at the U.S.National Institutes of Health and freely available on the internet atrsbweb (dot) nih (dot) gov/]. Images were captured in resolution of 10Mega Pixels (3888×2592 pixels) and stored in a low compression JPEG(Joint Photographic Experts Group standard) format. Next, analyzed datawas saved to text files and processed using the JMP statistical analysissoftware (SAS institute).

Seedling analysis—Using the digital analysis seedling data wascalculated, including leaf area, root coverage and root length.

The relative growth rate for the various seedling parameters wascalculated according to the following formulas XIII (RGR leaf area).XXVIII (RGR root coverage) and VI (RGR root length) as described above.

At the end of the experiment, plantlets were removed from the media andweighed for the determination of plant fresh weight. Plantlets were thendried for 24 hours at 60° C. and weighed again to measure plant dryweight for later statistical analysis. The fresh and dry weights wereprovided for each Arabidopsis plant. Growth rate was determined bycomparing the leaf area coverage, root coverage and root length, betweeneach couple of sequential photographs, and results were used to resolvethe effect of the gene introduced on plant vigor under optimalconditions. Similarly, the effect of the gene introduced on biomassaccumulation, under optimal conditions, was determined by comparing theplants' fresh and dry weight to that of control plants (containing anempty vector or the GUS reporter gene under the same promoter). Fromevery construct created, 3-5 independent transformation events wereexamined in replicates.

Statistical analyses—To identify genes conferring significantly improvedplant vigor or enlarged root architecture, the results obtained from thetransgenic plants were compared to those obtained from control plants.To identify outperforming genes and constructs, results from theindependent transformation events tested were analyzed separately. Toevaluate the effect of a gene event over a control the data was analyzedby Student's t-test and the p value was calculated. Results wereconsidered significant if p≤0.1. The JMP statistics software package isused (Version 5.2.1, SAS Institute Inc., Cary. N.C., USA).

Experimental Results:

Tables 116-118 summarize the observed phenotypes of transgenic plantsexpressing the gene constructs using the TC-T2 Assays.

The genes presented in Table 116 showed a significant improvement asthey produced larger plant biomass (plant fresh and dry weight) in T2generation when grown under normal growth conditions, compared tocontrol plants. The genes were cloned under the regulation of aconstitutive promoter (At6669. SEQ ID NO: 8190). The evaluation of eachgene was carried out by testing the performance of different number ofevents. Some of the genes were evaluated in more than one tissue cultureassay. The results obtained in these second experiments weresignificantly positive as well.

TABLE 116 Genes showing improved plant performance at Normal growthconditions under regulation of At6669 promoter Dry Weight [mg] FreshWeight [mg] P- % P- % Gene Name Event # Ave. Val. Incr. Ave. Val. Incr.LYM1185 81024.4 — — — 136.8 0.28 19 LYM1155 82890.4 — — — 136.5 0.24 19CONT. — — — — 114.7 — — LYM1237 83878.2 4.65 0.06 70 100.3 0.09 46LYM1237 83880.3 3.55 0.16 30 78.4 0.08 14 LYM1237 83880.5 — — — 78.20.16 14 LYM1237 83882.1 5.95 L 117 114.1 0.03 66 LYM1237 83882.2 5.300.01 94 107.7 0.01 57 LYM1230 80489.3 4.35 0.14 59 92.5 0.16 35 LYM123080490.1 4.95 L 81 108.6 0.05 58 LYM1230 80490.2 5.15 L 88 98.4 L 43LYM1230 80493.5 3.58 0.07 31 81.1 0.03 18 LYM1230 80493.7 3.58 0.11 3177.0 0.73 12 LYM1225 82566.1 4.33 0.15 58 98.2 0.02 43 LYM1225 82566.23.75 0.24 37 86.7 0.05 26 LYM1225 82566.6 4.57 0.02 67 102.2 0.04 49LYM1225 82566.8 3.73 0.06 36 — — — LYM1186 83933.1 5.23 L 91 121.6 0.0577 LYM1186 83933.2 3.65 0.11 33 83.1 0.19 21 LYM1186 83933.3 5.85 L 114109.2 L 59 LYM1186 83933.4 4.53 0.02 65 87.7 0.15 28 LYM1186 83937.15.25 0.03 92 97.5 0.10 42 LYM1185 81023.4 4.75 0.09 74 91.6 0.09 33LYM1185 81024.3 5.20 L 90 94.4 L 37 LYM1185 81025.2 3.75 0.06 37 86.80.16 26 LYM1185 81025.3 3.92 0.04 43 78.9 0.07 15 LYM1172 82555.3 5.25 L92 94.6 0.06 38 LYM1172 82555.5 4.28 0.18 56 93.7 0.19 37 LYM117282555.6 4.28 0.06 56 95.0 0.03 39 LYM1172 82558.10 5.22 0.02 91 94.20.05 37 LYM1172 82558.3 4.38 0.06 60 91.2 0.07 33 LYM1091_H5 83925.65.12 L 87 101.0 L 47 LYM1091_H5 83927.3 3.70 0.13 35 75.0 0.28 9LYM1091_H5 83927.4 4.60 L 68 105.5 L 54 LYM1091_H5 83927.5 4.53 0.04 65114.6 0.05 67 LYM1091_H5 83927.6 4.45 0.07 63 99.3 0.02 45 LYM102482475.4 4.17 0.04 53 93.2 0.11 36 LYM1024 82477.2 — — — 76.4 0.19 11LYM1024 82479.3 4.50 0.03 64 88.1 0.08 28 CONT. — 2.74 — — 68.6 — —LYM1186 83933.1 2.77 0.25 12 53.4 0.18 15 LYM1186 83933.2 4.55 L 84 79.10.02 70 LYM1186 83933.3 3.67 0.02 48 80.2 0.02 72 LYM1186 83933.4 3.170.02 28 56.3 0.08 21 LYM1186 83937.1 4.60 0.02 86 100.2 0.03 115LYM1076_H4 83975.2 3.28 L 32 58.3 0.18 25 LYM1076_H4 83976.1 3.25 0.0731 72.9 L 57 LYM1076_H4 83976.3 2.73 0.09 10 59.4 0.03 28 LYM1076_H483977.3 3.73 0.04 51 74.3 0.05 60 LYM1076_H4 83977.5 4.85 0.14 96 95.10.12 104 CONT. — 2.48 — — 46.5 — — LYM1161 80178.3 — — — 78.3 0.20 14LYM1161 80178.4 4.98 0.29 32 101.8 0.09 48 LYM1161 80179.1 5.83 0.25 55115.6 0.14 68 LYM1157 82231.2 — — — 74.5 0.27 9 LYM1157 82231.4 6.95 L85 132.0 L 92 LYM1157 82232.2 5.62 0.04 50 109.3 0.05 59 LYM1132 82013.1— — — 89.8 0.02 31 LYM1132 82013.4 — — — 81.5 0.13 19 LYM1132 82017.64.68 0.22 24 89.0 0.04 30 LYM1124 82008.4 — — — 95.7 0.07 39 LYM111580133.1 4.20 0.25 12 80.8 0.13 18 LYM1115 80134.2 4.33 0.14 15 89.4 L 30LYM1115 80135.2 6.58 0.20 75 129.1 0.13 88 LYM1085 82683.2 6.98 0.24 85134.6 0.21 96 LYM1085 82683.3 4.70 0.20 25 91.7 0.13 34 LYM1085 82685.14.70 0.10 25 89.9 L 31 LYM1085 82685.12 4.55 0.28 21 95.0 L 38 LYM108282481.2 — — — 80.4 0.24 17 LYM1082 82481.4 — — — 80.4 0.22 17 LYM108282482.5 — — — 76.0 0.17 11 LYM1073 80978.3 5.38 L 43 93.3 0.05 36LYM1073 80978.4 5.03 0.03 34 101.6 0.02 48 LYM1073 80980.3 — — — 91.20.15 33 LYM1073 80981.1 4.55 0.22 21 90.0 0.17 31 LYM1054 80106.2 — — —76.1 0.21 11 LYM1054 80108.2 5.60 0.01 49 — — — LYM1054 80110.4 5.650.20 50 120.4 0.09 75 LYM1044 82612.2 5.70 0.07 51 115.7 0.16 69 LYM104482613.2 — — — 82.1 0.07 20 LYM1044 82613.4 4.80 0.06 28 88.4 0.03 29LYM1044 82614.1 — — — 79.3 0.25 15 LYM1042 82000.2 — — — 88.5 0.10 29LYM1042 82001.2 — — — 85.5 0.10 25 LYM1029 81349.4 — — — 86.2 0.04 26LYM1029 81351.4 4.90 0.13 30 98.2 0.03 43 LYM1029 81353.1 — — — 84.40.04 23 LYM1029 81353.3 — — — 97.9 0.10 43 CONT. — 3.76 — — 68.6 — —LYM1229 81574.1 5.30 L 75 107.4 L 95 LYM1229 81574.2 4.00 0.18 32 80.30.07 46 LYM1229 81575.1 4.47 0.01 48 116.8 0.23 112 LYM1229 81575.3 5.50L 82 112.5 L 104 LYM1229 81576.5 — — — 79.4 0.23 44 LYM1227 81179.1 4.280.02 41 76.6 L 39 LYM1227 81179.2 4.40 0.18 45 106.6 0.02 94 LYM122781179.7 4.68 0.13 55 97.9 0.09 78 LYM1227 81180.2 5.95 L 97 155.0 0.02181 LYM1219 80509.2 5.77 L 91 100.3 0.04 82 LYM1219 80510.3 4.12 L 3680.7 L 46 LYM1219 80510.4 5.80 0.06 92 154.2 0.24 180 LYM1219 80513.25.03 0.01 66 102.3 L 86 LYM1218 81114.1 4.22 L 40 75.6 0.03 37 LYM121881114.2 7.30 L 141 176.2 L 220 LYM1218 81116.6 3.73 0.06 23 — — —LYM1218 81116.7 6.58 0.13 117 168.7 0.12 206 LYM1218 81116.8 6.10 L 102176.9 0.06 221 LYM1216 80256.7 6.00 L 98 115.0 L 109 LYM1216 80259.14.72 0.03 56 125.7 0.16 128 LYM1216 80259.2 4.47 L 48 78.5 0.02 43LYM1216 80260.1 6.58 L 117 116.0 L 111 LYM1212 80777.1 3.47 0.29 15 96.90.14 76 LYM1212 80779.1 5.30 L 75 94.7 L 72 LYM1210 80617.1 — — — 80.20.29 46 LYM1210 80618.2 4.15 0.02 37 80.5 0.07 46 LYM1210 80618.3 5.700.03 88 119.5 0.01 117 LYM1210 80620.2 5.62 0.02 86 134.2 L 144 LYM121080620.5 4.72 0.06 56 105.0 L 91 LYM1209 80479.1 5.95 L 97 144.4 0.10 162LYM1209 80480.1 7.17 L 137 136.7 L 148 LYM1209 80482.3 3.75 0.05 24 72.30.01 31 LYM1209 80482.4 4.80 0.02 59 100.2 0.06 82 LYM1209 80482.6 4.980.02 64 139.5 0.09 153 LYM1201 81102.1 4.42 0.05 46 81.4 0.06 48 LYM120181102.3 3.73 0.08 23 — — — LYM1201 81103.1 3.78 0.02 25 80.1 L 45LYM1201 81104.4 6.12 0.03 102 164.4 0.15 199 LYM1201 81105.2 5.30 0.0675 98.8 0.01 79 LYM1195 81918.3 5.75 0.04 90 115.2 0.02 109 LYM119581919.3 5.45 0.05 80 127.2 0.19 131 LYM1195 81919.4 5.50 L 82 108.5 0.0297 LYM1195 81919.6 5.05 0.01 67 113.4 0.07 106 LYM1189 81099.1 4.72 0.0256 97.2 L 76 LYM1189 81099.2 4.60 0.04 52 85.9 L 56 LYM1189 81100.2 3.920.16 30 70.8 0.12 29 LYM1189 81101.4 — — — 66.8 0.09 21 LYM1182 81492.15.83 0.07 93 120.8 0.07 119 LYM1182 81492.4 8.08 L 167 164.4 L 198LYM1182 81493.2 5.55 L 83 107.1 L 95 LYM1182 81494.2 4.17 0.05 38 85.80.02 56 LYM1182 81495.5 4.92 0.02 63 98.1 0.02 78 LYM1176 80809.1 3.600.29 19 69.0 0.03 25 LYM1176 80810.3 4.03 0.03 33 74.9 0.03 36 LYM117680810.6 460 0.06 52 92.1 0.02 67 LYM1176 80811.3 5.93 0.05 96 181.3 0.27229 LYM1176 80811.4 — — — 72.5 0.15 32 LYM1173 80464.1 9.03 L 198 165.0L 200 LYM1173 80464.4 7.50 L 148 147.4 L 168 LYM1173 80464.5 4.25 0.1340 119.7 0.02 117 LYM1173 80466.2 — — — 98.8 0.07 79 LYM1170 80187.14.77 0.06 58 84.4 0.10 53 LYM1170 80187.5 3.88 0.09 28 78.0 0.11 42LYM1170 80189.1 4.22 0.08 40 117.7 0.11 114 LYM1170 80189.3 5.38 0.09 78107.1 L 95 LYM1170 80189.4 4.95 L 64 125.7 0.06 128 LYM1169 80606.4 6.330.02 109 110.4 L 100 LYM1169 80607.2 — — — 72.0 0.13 31 LYM1169 80607.35.22 L 73 107.1 L 95 LYM1146 81487.4 7.00 L 131 164.9 0.03 199 LYM114681487.5 5.00 0.01 65 131.9 0.07 139 LYM1146 81488.2 7.60 L 151 149.0 L171 LYM1146 81491.4 5.42 L 79 139.2 0.03 153 LYM1146 81491.5 7.55 0.01150 148.4 0.03 169 CONT. — 3.02 — — 55.1 — — LYM1208 83928.4 4.38 L 67108.6 0.30 49 LYM1208 83928.5 3.20 0.18 22 — — — LYM1208 83929.5 3.980.05 52 106.7 0.20 46 LYM1208 83929.6 4.92 L 89 104.5 0.09 43 LYM120583050.6 5.77 L 121 130.3 L 79 LYM1205 83054.3 4.42 L 69 — — — LYM117483270.2 3.15 0.21 21 — — — LYM1174 83274.1 5.22 0.03 100 94.5 0.25 30LYM1174 83274.5 8.18 L 213 172.4 0.03 136 LYM1160 81861.2 3.70 0.09 4290.0 0.24 23 LYM1160 81862.3 5.47 L 110 103.9 0.09 43 LYM1153 83265.54.77 0.09 82 103.9 0.19 43 LYM1153 83267.2 4.12 L 58 139.4 0.12 91LYM1153 83267.4 5.60 L 114 109.7 0.07 50 LYM1153 83267.5 5.45 0.01 109112.9 0.04 55 LYM1125 83041.4 5.68 0.04 117 105.4 0.16 45 LYM112583042.1 3.17 0.06 22 — — — LYM1125 83042.5 5.35 0.20 105 115.3 0.20 58LYM1122 81983.5 3.55 0.06 36 — — — LYM1122 81983.6 5.92 0.02 127 129.30.04 77 LYM1122 81985.2 3.55 0.13 36 104.2 0.18 43 LYM1122 81985.4 4.450.03 70 134.6 0.14 85 LYM1122 81985.5 5.20 L 99 101.5 0.08 39 LYM109083375.3 2.95 0.29 13 — — — LYM1090 83377.1 3.78 0.07 44 — — — LYM109083379.3 4.77 0.12 83 118.5 0.03 62 LYM1090 83379.4 3.52 0.07 35 — — —LYM1090 83379.6 4.72 L 81 — — — LYM1088 83557.1 7.58 L 190 170.4 L 134LYM1088 83557.2 5.83 0.03 123 127.0 0.10 74 LYM1088 83558.1 3.85 0.23 47— — — LYM1088 83558.4 6.05 L 132 141.5 L 94 LYM1088 83558.5 4.22 0.04 62— — — LYM1046 81907.2 3.10 0.22 19 86.7 0.27 19 LYM1046 81909.2 3.380.14 29 — — — LYM1046 81909.4 3.12 0.21 20 — — — LYM1046 81909.5 4.72 L81 96.4 0.17 32 LYM1035 83260.3 5.25 0.14 101 — — — LYM1035 83260.5 4.470.03 71 103.1 0.12 41 LYM1035 83262.1 4.90 L 88 110.9 0.01 52 LYM103583264.6 3.20 0.12 22 — — — LYM1030 83544.1 3.88 0.09 48 — — — LYM103083547.2 3.85 0.05 47 98.5 0.29 35 CONT. — 2.61 — — 72.9 — — LYM111882534.6 4.33 0.24 26 97.9 0.23 27 LYM1114 82487.11 4.22 0.08 23 96.50.17 25 LYM1114 82487.7 4.93 0.03 44 156.4 0.06 102 LYM1113 81872.4 5.30L 55 125.4 L 62 LYM1106 82627.2 5.77 0.02 68 109.7 0.21 42 LYM110682627.6 4.15 0.28 21 — — — LYM1078 82657.6 4.77 0.17 39 131.0 L 70LYM1078 82657.9 5.57 L 63 125.2 L 62 LYM1078 82659.2 6.10 L 78 118.60.04 53 LYM1078 82659.5 5.00 0.13 46 101.6 0.07 32 LYM1075 83047.2 4.980.03 45 117.4 0.03 52 LYM1075 83047.8 4.37 0.19 27 — — — LYM1075 83049.15.30 0.03 55 113.1 0.05 46 LYM1075 83049.3 4.93 0.01 44 105.8 0.09 37LYM1074 82571.1 5.25 0.02 53 121.0 0.07 57 LYM1074 82572.2 5.20 L 52111.2 0.07 44 LYM1066 82003.1 4.33 0.17 27 — — — LYM1033 82883.6 4.950.04 45 112.5 0.02 46 LYM1033 82885.5 4.95 0.02 45 99.4 0.09 29 LYM102782520.3 6.65 L 94 160.9 0.01 108 LYM1027 82520.6 5.07 0.02 48 99.5 0.1629 LYM1027 82523.1 4.40 0.14 28 — — — LYM1027 82523.2 4.25 0.07 24 115.80.16 50 LYM1024 82475.5 — — — 116.2 0.04 50 LYM1024 82477.2 5.73 0.22 67— — — LYM1023 82516.1 5.30 L 55 117.5 0.06 52 LYM1023 82518.10 4.62 0.0735 — — — CONT. — 3.43 — — 77.3 — — Table 116. “CONT.”-Control;“Ave.”-Average; “% Incr.” = % increment; “p-val.”- p-value, L-p < 0.01.

The genes presented in Tables 117 and 118 show a significant improvementin plant performance since they produced a larger leaf biomass(leafarea) and root biomass (root length and root coverage) (Table 117) and ahigher relative growth rate of leaf area, root coverage and root length(Table 118) when grown under normal growth conditions, compared tocontrol plants. Plants producing larger root biomass have betterpossibilities to absorb larger amount of nitrogen from soil. Plantsproducing larger leaf biomass have better ability to produceassimilates. The genes were cloned under the regulation of aconstitutive promoter (At6669). The evaluation of each gene wasperformed by testing the performance of different number of events. Someof the genes were evaluated in more than one tissue culture assay. Thissecond experiment confirmed the significant increment in leaf and rootperformance. Event with p-value <0.1 was considered statisticallysignificant.

TABLE 117 Genes showing improved plant performance at Normal growthconditions under regulation of At6669 promoter Leaf Area Roots CoverageRoots Length [cm²] [cm²] [cm] P- % P- % P- % Gene Name Event # Ave. Val.Incr. Ave. Val. Incr. Ave. Val. Incr. LYM1228 82560.2 0.643 0.28 15 — —— 7.34 0.30 3 LYM1226 82893.10 — — — — — — 7.31 0.25 3 LYM1226 82893.5 —— — — — — 7.69 0.02 9 LYM1225 82564.2 — — — — — — 7.32 0.24 3 LYM122582566.1 — — — 9.36 0.19 20 — — — LYM1225 82566.6 — — — — — — 7.44 0.08 5LYM1207 80247.4 — — — — — — 7.57 0.10 7 LYM1203 81750.1 — — — — — — 7.490.27 6 LYM1185 81024.4 — — — 9.13 0.15 17 7.30 0.26 3 LYM1178 82652.6 —— — — — — 7.59 0.10 7 LYM1172 82555.5 — — — — — — 7.40 0.21 4 LYM117282558.3 0.642 0.22 15 9.82 0.25 26 7.99 L 13 LYM1155 82888.5 0.689 0.2423 — — — — — — LYM1155 82890.4 0.706 0.04 26 9.49 0.19 22 — — — LYM115582890.5 — — — — — — 7.43 0.11 5 LYM1128 82548.1 — — — 10.1 0.15 30 7.630.05 8 LYM1070 82533.5 — — — — — — 7.38 0.20 4 LYM1070 82533.8 — — — — —— 7.47 0.06 5 LYM1059_H7 82876.9 — — — 9.86 0.29 26 — — — LYM103782524.1 — — — — — — 7.58 0.02 7 CONT. — 0.560 — — 7.79 — — 7.09 — —LYM1237 83878.2 0.531 0.18 26 — — — — — — LYM1237 83880.3 0.446 0.29 6 —— — — — — LYM1237 83882.1 0.627 L 49 6.54 0.02 26 — — — LYM1237 83882.20.590 0.01 41 7.30 L 40 7.19 0.04 11 LYM1230 80490.1 0.509 L 21 6.550.04 26 — — — LYM1230 80490.2 0.586 L 40 5.92 0.11 14 — — — LYM123080493.5 0.456 0.04 9 — — — — — — LYM1225 82566.1 0.571 0.04 36 6.62 0.0527 7.07 0.09 9 LYM1225 82566.2 0.545 0.03 30 6.49 0.11 25 7.12 0.15 10LYM1225 82566.6 0.492 0.10 17 6.42 0.05 23 — — — LYM1225 82566.8 0.4620.24 10 — — — — — — LYM1186 83933.1 0.638 0.02 52 7.46 L 43 7.10 0.16 10LYM1186 83933.3 0.605 L 44 6.75 0.06 30 — — — LYM1186 83933.4 0.491 0.1017 — — — — — — LYM1186 83937.1 0.555 0.02 32 6.37 0.07 23 — — — LYM118581023.4 0.488 0.25 16 — — — — — — LYM1185 81024.3 0.540 0.03 29 6.500.08 25 7.14 0.10 10 LYM1185 81025.3 0.467 0.04 11 — — — — — — LYM117282555.3 0.523 L 25 — — — — — — LYM1172 82555.5 0.507 0.16 21 7.15 0.0537 7.35 L 13 LYM1172 82555.6 0.521 0.04 24 — — — — — — LYM1172 82558.100.505 0.07 20 6.19 0.24 19 — — — LYM1172 82558.3 0.510 0.01 22 — — — — —— LYM1091_H5 83925.6 0.546 L 30 7.30 L 40 7.50 0.02 16 LYM1091_H583927.4 0.570 0.03 36 7.56 0.01 45 7.42 0.01 14 LYM1091_H5 83927.5 0.5280.07 26 6.43 0.12 24 7.02 0.27 8 LYM1091_H5 83927.6 0.508 0.17 21 6.310.18 21 — — — CONT. — 0.420 — — 5.20 — — 6.48 — — LYM1186 83933.1 0.343L 17 4.96 0.01 18 6.25 0.03 11 LYM1186 83933.2 0.423 L 44 6.42 L 52 6.210.07 10 LYM1186 83933.3 0.447 L 52 6.51 L 54 7.28 L 30 LYM1186 83933.40.340 L 16 4.58 0.17 9 — — — LYM1186 83937.1 0.482 0.03 64 6.77 L 617.18 L 28 LYM1076_H4 83975.2 0.355 L 21 — — — — — — LYM1076_H4 83976.10.402 0.01 37 5.14 0.29 22 6.66 0.01 19 LYM1076_H4 83976.3 0.349 0.03 195.43 0.07 29 6.63 0.03 18 LYM1076_H4 83977.3 0.399 0.03 36 — — — — — —LYM1076_H4 83977.5 0.520 0.08 77 5.89 0.15 40 6.51 0.14 16 CONT. — 0.294— — 4.22 — — 5.62 — — LYM1161 80178.3 — — — 5.37 0.09 18 6.40 0.12 10LYM1161 80178.4 0.463 0.14 28 — — — — — — LYM1161 80179.1 0.540 0.08 506.50 0.25 43 — — — LYM1161 80179.3 — — — — — — 6.67 0.08 14 LYM115782231.2 0.385 0.13 7 5.06 0.26 11 6.55 0.12 12 LYM1157 82231.4 0.5480.02 52 6.86 0.08 50 — — — LYM1157 82232.2 0.468 0.11 30 — — — — — —LYM1132 82013.1 0.476 L 32 — — — 6.49 0.08 11 LYM1132 82013.4 0.437 L 21— — — 6.73 0.02 16 LYM1132 82017.6 0.412 0.15 14 — — — — — — LYM112482008.4 0.450 0.01 25 6.00 0.12 32 6.35 0.26 9 LYM1115 80133.1 0.452 L25 — — — 6.82 0.06 17 LYM1115 80133.6 0.412 0.14 14 — — — — — — LYM111580134.2 0.463 0.03 28 5.89 0.02 29 7.31 L 25 LYM1115 80135.2 0.564 0.1156 6.90 0.24 51 6.47 0.05 11 LYM1085 82683.2 0.576 0.12 60 — — — — — —LYM1085 82683.3 0.437 0.21 21 6.04 0.07 32 6.25 0.20 7 LYM1085 82685.10.488 0.02 35 5.96 0.18 31 6.73 0.18 15 LYM1085 82685.12 0.447 0.03 245.47 0.23 20 7.14 L 22 LYM1082 82481.2 0.410 0.14 14 — — — — — — LYM108282481.4 — — — 5.24 0.08 15 — — — LYM1082 82482.4 — — — 5.08 0.26 11 6.960.02 19 LYM1082 82482.5 0.397 0.04 10 — — — — — — LYM1073 80978.3 0.4690.02 30 — — — — — — LYM1073 80978.4 0.430 L 19 — — — — — — LYM107380980.3 0.390 0.25 8 — — — — — — LYM1073 80981.1 0.490 0.05 36 — — — — —— LYM1054 80106.2 0.436 L 21 — — — 6.52 0.02 12 LYM1054 80108.2 0.491 L36 — — — — — — LYM1054 80110.4 0.506 0.09 40 — — — — — — LYM1044 82612.20.490 0.10 36 — — — — — — LYM1044 82613.4 0.457 L 27 6.00 0.03 31 6.750.11 16 LYM1044 82614.1 0.425 0.06 18 — — — — — — LYM1042 81998.2 0.4020.23 11 — — — 6.98 L 20 LYM1042 82000.2 0.415 0.10 15 — — — — — —LYM1042 82000.4 0.418 L 16 — — — 6.47 0.07 11 LYM1042 82001.2 0.429 0.1819 — — — — — — LYM1029 81349.4 0.417 0.09 16 — — — — — — LYM1029 81351.40.422 0.15 17 6.79 0.03 49 6.99 0.03 20 LYM1029 81353.1 0.424 0.08 185.26 0.23 15 6.50 0.22 12 CONT. — 0.361 — — 4.56 — — 5.83 — — LYM122981574.1 0.523 L 77 9.53 0.01 137 7.66 L 28 LYM1229 81574.2 0.433 0.02 477.72 0.05 92 7.35 0.02 23 LYM1229 81575.1 0.413 L 40 5.92 0.06 47 — — —LYM1229 81575.3 0.577 L 96 9.24 L 130 7.33 L 22 LYM1229 81576.5 0.373 L27 5.19 0.07 29 — — — LYM1227 81179.1 0.408 L 38 6.01 0.01 49 6.80 0.0814 LYM1227 81179.2 0.471 L 60 7.52 L 87 7.34 L 23 LYM1227 81179.3 0.374L 27 5.73 0.03 42 6.81 0.13 14 LYM1227 81179.7 0.449 0.12 52 7.16 0.1478 — — — LYM1227 81180.2 0.541 L 84 9.11 L 127 7.36 0.02 23 LYM121980509.2 0.548 L 86 7.06 0.01 76 7.02 0.03 17 LYM1219 80510.3 0.488 L 667.46 L 85 7.39 0.02 23 LYM1219 80510.4 0.504 0.03 71 6.74 0.01 68 — — —LYM1219 80513.2 0.506 0.01 72 8.61 0.04 114 7.79 L 30 LYM1218 81114.10.391 L 33 7.34 0.03 82 6.79 0.25 13 LYM1218 81114.2 0.712 L 142 9.490.05 136 7.46 0.01 25 LYM1218 81116.6 0.349 0.02 18 5.68 0.08 41 6.870.08 15 LYM1218 81116.7 0.544 0.13 84 8.61 0.01 114 7.19 0.01 20 LYM121881116.8 0.675 L 129 9.03 L 125 7.73 L 29 LYM1216 80256.4 0.332 0.24 13 —— — — — — LYM1216 80256.7 0.561 L 90 9.87 0.03 145 7.48 0.02 25 LYM121680259.1 0.486 L 65 6.38 L 59 6.97 0.07 16 LYM1216 80259.2 0.447 L 526.99 L 74 7.06 0.02 18 LYM1216 80260.1 0.582 L 97 9.75 L 142 7.74 L 29LYM1212 80777.1 0.414 L 41 5.00 0.15 24 — — — LYM1212 80779.1 0.538 L 83— — — — — — LYM1210 80617.1 0.349 L 18 5.53 0.07 37 — — — LYM121080618.2 0.444 L 51 5.68 0.14 41 — — — LYM1210 80618.3 0.600 L 103 8.93 L122 7.76 L 30 LYM1210 80620.2 0.567 L 92 7.60 0.06 89 7.53 L 26 LYM121080620.5 0.493 0.02 67 6.25 0.13 55 — — — LYM1209 80479.1 0.524 L 78 8.37L 108 7.22 0.02 21 LYM1209 80480.1 0.661 L 124 8.89 0.01 121 7.25 0.0521 LYM1209 80482.3 0.386 L 31 4.83 0.21 20 — — — LYM1209 80482.4 0.489 L66 5.51 0.08 37 — — — LYM1209 80482.6 0.449 L 52 6.93 L 72 6.51 0.22 9LYM1201 81102.1 0.412 L 40 7.13 L 77 7.50 L 25 LYM1201 81102.3 0.3670.08 25 5.42 0.03 35 6.68 0.13 12 LYM1201 81103.1 0.430 L 46 6.83 L 707.37 L 23 LYM1201 81104.4 0.587 0.03 99 6.81 0.06 69 — — — LYM120181105.2 0.483 0.01 64 6.94 0.03 73 6.79 0.09 13 LYM1195 81918.1 0.3350.18 14 — — — — — — LYM1195 81918.3 0.578 L 96 10.3 0.03 156 8.03 L 34LYM1195 81919.3 0.567 L 92 7.89 0.01 96 7.36 0.02 23 LYM1195 81919.40.575 L 95 7.02 L 75 6.94 0.04 16 LYM1195 81919.6 0.538 0.02 83 7.040.09 75 7.45 0.11 24 LYM1189 81097.2 — — — 5.42 0.14 35 6.67 0.19 11LYM1189 81099.1 0.460 L 56 6.01 0.06 49 — — — LYM1189 81099.2 0.460 L 567.54 L 88 7.55 L 26 LYM1189 81100.2 0.389 L 32 6.39 0.09 59 6.75 0.20 13LYM1189 81101.4 0.391 0.03 33 5.12 0.21 27 6.91 0.13 15 LYM1182 81492.10.509 0.02 73 6.13 0.12 52 6.86 0.21 14 LYM1182 81492.4 0.740 L 151 11.8L 192 7.89 L 32 LYM1182 81493.2 0.496 0.03 68 7.26 0.01 81 6.94 0.18 16LYM1182 81494.2 0.388 0.02 32 6.95 L 73 6.70 0.19 12 LYM1182 81495.50.474 0.04 61 7.02 0.02 75 — — — LYM1176 80809.1 0.326 0.09 11 — — — — —— LYM1176 80810.3 0.412 L 40 5.03 0.09 25 6.45 0.27 8 LYM1176 80810.60.467 L 58 5.92 0.06 47 6.42 0.28 7 LYM1176 80811.3 0.526 0.10 78 8.450.09 110 — — — LYM1176 80811.4 0.381 0.02 29 6.36 0.15 58 — — — LYM117380464.1 0.724 L 146 14.1 L 251 7.78 L 30 LYM1173 80464.4 0.663 L 1259.93 L 147 — — — LYM1173 80464.5 0.455 0.04 54 8.45 0.03 110 — — —LYM1173 80466.2 0.394 0.02 34 8.68 0.02 116 7.15 0.09 19 LYM1170 80187.10.449 0.10 52 5.71 0.11 42 — — — LYM1170 80187.5 0.446 L 51 6.03 0.05 506.97 0.11 16 LYM1170 80189.1 0.419 L 42 7.59 L 89 6.96 0.07 16 LYM117080189.3 0.455 L 54 7.64 0.06 90 — — — LYM1170 80189.4 0.465 0.01 58 8.03L 100 7.42 0.02 24 LYM1169 80606.4 0.501 0.04 70 7.74 0.07 93 — — —LYM1169 80607.2 0.328 0.25 11 5.29 0.15 32 — — — LYM1169 80607.3 0.456 L55 8.76 0.02 118 7.36 0.07 23 LYM1146 81487.4 0.645 0.01 119 6.86 0.0370 — — — LYM1146 81487.5 0.460 L 56 7.61 L 89 7.59 L 27 LYM1146 81488.20.691 L 134 11.2 L 179 7.99 L 33 LYM1146 81491.4 0.536 L 82 5.05 0.09 25— — — LYM1146 81491.5 0.673 0.01 128 8.02 L 99 6.93 0.12 16 CONT. —0.295 — — 4.02 — — 5.99 — — LYM1208 83928.4 0.516 0.04 45 6.65 0.04 31 —— — LYM1208 83928.5 0.392 0.27 10 — — — — — — LYM1208 83929.5 0.431 0.0521 — — — — — — LYM1208 83929.6 0.510 0.02 43 6.23 0.28 23 — — — LYM120583050.6 0.606 L 70 7.67 L 52 — — — LYM1205 83054.3 0.498 0.02 40 6.430.04 27 — — — LYM1174 83270.2 0.382 0.26 7 — — — — — — LYM1174 83274.10.577 0.02 62 7.82 0.16 54 — — — LYM1174 83274.5 0.7.54 L 106 7.42 0.0546 — — — LYM1160 81861.2 0.440 0.14 24 6.67 0.28 32 — — — LYM116081862.3 0.597 0.02 68 9.40 0.01 86 8.18 0.01 16 LYM1160 81862.5 — — —6.26 0.19 24 7.51 0.16 6 LYM1153 83265.5 0.517 L 45 7.47 0.21 47 — — —LYM1153 83267.2 0.452 L 27 — — — — — — LYM1153 83267.4 0.593 L 67 7.500.07 48 — — — LYM1153 83267.5 0.498 0.07 40 — — — — — — LYM1153 83269.50.403 0.15 13 — — — — — — LYM1125 83041.4 0.589 0.06 65 6.97 0.23 38 — —— LYM1125 83042.1 0.446 L 25 5.71 0.25 13 — — — LYM1125 83042.5 0.5730.13 61 — — — — — — LYM1122 81983.5 0.417 0.24 17 — — — — — — LYM112281983.6 0.648 0.01 82 8.24 0.09 63 — — — LYM1122 81985.2 0.507 L 42 6.600.05 30 — — — LYM1122 81985.4 0.474 0.03 33 — — — — — — LYM1122 81985.50.507 L 42 — — — — — — LYM1090 83377.1 0.439 0.18 23 6.69 0.06 32 — — —LYM1090 83379.3 0.558 0.04 57 — — — — — — LYM1090 83379.4 0.453 0.03 276.86 0.12 36 7.63 0.13 8 LYM1090 83379.6 0.522 0.04 47 6.76 0.14 34 — —— LYM1088 83557.1 0.747 L 110 9.98 0.02 97 7.67 0.20 8 LYM1088 83557.20.611 L 72 8.41 0.06 66 8.01 L 13 LYM1088 83558.1 0.505 0.08 42 7.630.14 51 — — — LYM1088 83558.4 0.638 L 79 8.74 0.02 73 — — — LYM108883558.5 0.476 L 34 6.45 0.06 27 — — — LYM1046 81907.2 0.456 0.03 28 — —— — — — LYM1046 81907.4 0.421 0.08 18 — — — — — — LYM1046 81909.2 0.4140.05 16 — — — — — — LYM1046 81909.4 0.403 0.20 13 — — — — — — LYM104681909.5 0.555 L 56 — — — — — — LYM1035 83260.3 0.493 0.21 39 7.96 0.2257 — — — LYM1035 83260.5 0.527 0.03 48 8.02 0.11 58 — — — LYM103583262.1 0.531 L 49 7.67 L 52 — — — LYM1030 83544.1 0.399 0.26 12 — — — —— — LYM1030 83544.3 0.417 0.04 17 — — — — — — LYM1030 83547.2 0.448 0.0226 7.34 L 45 — — — CONT. — 0.356 — — 5.06 — — 7.08 — — LYM1118 82534.60.432 0.13 26 — — — — — — LYM1114 82487.11 0.472 L 38 5.03 0.18 21 6.340.21 10 LYM1114 82487.7 0.476 L 39 5.98 0.07 44 6.62 0.21 15 LYM111381872.4 0.489 L 43 6.01 0.02 45 7.05 0.02 22 LYM1106 82627.2 0.545 0.0859 6.00 0.02 45 — — — LYM1106 82627.3 — — — 5.15 0.16 24 — — — LYM110682627.6 0.399 0.19 16 4.90 0.25 18 — — — LYM1078 82657.6 0.446 0.10 30 —— — — — — LYM1078 82657.9 0.518 L 51 7.22 L 74 6.83 0.06 19 LYM107882659.2 0.598 L 75 7.02 L 69 7.49 L 30 LYM1078 82659.5 0.438 0.03 28 — —— — — — LYM1075 83047.2 0.472 0.01 38 5.55 0.05 34 6.63 0.10 15 LYM117583047.8 0.429 0.04 25 — — — — — — LYM1075 83049.1 0.513 0.04 50 6.350.06 53 6.84 0.07 19 LYM1075 83049.3 0.522 0.03 52 6.43 0.04 55 — — —LYM1074 82571.1 0.427 0.08 25 5.08 0.18 22 — — — LYM1074 82572.2 0.4530.01 32 5.00 0.22 21 — — — LYM1066 82003.1 0.419 0.07 22 — — — — — —LYM1033 82883.6 0.445 0.04 30 6.46 L 56 6.53 0.13 13 LYM1033 82885.50.408 0.11 19 5.63 0.06 36 — — — LYM1027 82520.3 0.664 L 94 6.67 0.06 61— — — LYM1027 82520.6 0.477 0.04 39 — — — — — — LYM1027 82523.1 0.4040.22 18 — — — — — — LYM1027 82523.2 0.394 0.26 15 — — — — — — LYM102482475.5 0.441 0.04 29 — — — — — — LYM1023 82516.1 0.549 0.02 60 5.970.28 44 — — — LYM1023 82518.1 0.401 0.19 17 — — — — — — LYM1023 82518.100.429 0.06 25 — — — — — — CONT. — 0.342 — — 4.15 — — 5.76 — — Table 117.“CONT.”-Control; “Ave.”-Average; “% Incr.” = % increment; “p-val.”-p-value, L-p < 0.0.

TABLE 118 Genes showing improved plant performance at Normal growthconditions under regulation of At6669 promoter RGR Of RGR Of Roots RGROf Leaf Area Coverage Root Length % % % Gene Name Event # Ave. P-Val.Incr. Ave. P-Val. Incr. Ave. P-Val. Incr. LYM1228 82560.2 — — — — — —0.660 0.23 8 LYM1226 82893.10 — — — — — — 0.669 0.13 10 LYM1226 82893.5— — — — — — 0.725 L 19 LYM1225 82566.1 — — — 1.10 0.18 22 — — — LYM120780247.4 — — — — — — 0.674 0.10 10 LYM1194 81624.5 — — — — — — 0.659 0.298 LYM1192 81128.3 — — — — — — 0.666 0.19 9 LYM1185 81024.4 — — — 1.060.27 17 — — — LYM1178 82652.6 — — — — — — 0.686 0.10 13 LYM1172 82555.3— — — — — — 0.677 0.10 11 LYM1172 82558.3 — — — 1.17 0.13 29 0.740 L 21LYM1155 82888.5 0.0696 0.14 29 — — — — — — LYM1155 82890.4 0.0694 0.0928 1.14 0.13 26 0.672 0.14 10 LYM1155 82890.5 — — — — — — 0.655 0.29 7LYM1128 82548.1 0.0670 0.24 24 1.17 0.10 30 — — — LYM1059_H7 82876.9 — —— 1.14 0.18 26 — — — LYM1037 82524.3 — — — — — — 0.656 0.25 8 CONT. —0.0541 — — 0.902 — — 0.610 — — LYM1237 83878.2 0.0512 0.06 29 — — — — —— LYM1237 83882.1 0.0611 L 54 0.759 L 37 — — — LYM1237 83882.2 0.0558 L41 0.811 L 46 0.589 0.06 13 LYM1230 80489.3 0.0490 0.11 24 0.645 0.27 160.580 0.23 11 LYM1230 80490.1 0.0506 L 28 0.718 0.02 29 0.572 0.18 10LYM1230 80490.2 0.0539 L 36 0.658 0.10 19 — — — LYM1230 80493.5 — — — —— — 0.574 0.14 10 LYM1225 82566.1 0.0539 L 36 0.730 0.02 32 0.570 0.12 9LYM1225 82566.2 0.0501 0.02 27 0.725 0.02 31 — — — LYM1225 82566.60.0486 0.03 23 0.740 0.01 33 — — — LYM1225 82566.8 — — — 0.661 0.19 190.579 0.28 11 LYM1186 83933.1 0.0623 L 57 0.842 L 52 0.565 0.24 9LYM1186 83933.3 0.0590 L 49 0.781 L 41 0.595 0.10 14 LYM1186 83933.40.0475 0.07 20 — — — — — — LYM1186 83937.1 0.0500 0.02 26 0.732 0.01 32— — — LYM1185 81023.4 0.0493 0.05 25 0.692 0.12 25 — — — LYM1185 81024.30.0509 L 29 0.704 0.04 27 0.561 0.25 8 LYM1185 81024.4 — — — — — — 0.5660.15 9 LYM1185 81025.3 0.0464 0.07 17 — — — — — — LYM1172 82555.3 0.0517L 31 — — — — — — LYM1172 82555.5 0.0498 0.06 26 0.811 L 46 0.607 0.02 17LYM1172 82555.6 0.0514 L 30 0.659 0.15 19 0.633 L 22 LYM1172 82558.100.0493 0.02 24 0.686 0.09 24 — — — LYM1112 82558.3 0.0464 0.09 17 — — —— — — LYM1091_H5 83925.6 0.0533 L 35 0.858 L 55 0.592 0.04 14 LYM1091_H583927.3 — — — 0.626 0.25 13 — — — LYM1091_H5 83927.4 0.0558 L 41 0.840 L51 0.561 0.23 8 LYM1091_H5 83927.5 0.0504 0.03 27 0.740 0.01 33 0.5850.14 12 LYM1091_H5 83927.6 0.0486 0.08 23 0.682 0.10 23 — — — CONT. —0.0396 — — 0.555 — — 0.520 — — LYM1186 83933.1 0.0316 0.03 21 0.590 0.0623 0.546 0.13 13 LYM1186 83933.2 0.0404 L 54 0.777 L 63 0.587 0.02 22LYM1186 83933.3 0.0405 L 55 0.709 L 49 0.611 L 27 LYM1186 83933.4 0.03060.04 17 0.543 0.25 14 — — — LYM1186 83937.1 0.0431 L 65 0.726 L 52 0.615L 28 LYM1076_H4 83975.2 0.0319 0.01 22 — — — — — — LYM1076_H4 83976.10.0323 0.03 24 — — — — — — LYM1076_H4 83976.3 0.0316 0.05 21 0.648 0.0236 0.579 0.05 20 LYM1076_H4 83977.3 0.0371 L 42 0.562 0.23 18 — — —LYM1076_H4 83977.5 0.0478 L 83 0.666 0.05 40 0.587 0.07 22 CONT. —0.0262 — — 0.478 — — 0.482 — — LYM1161 80178.3 0.0385 0.06 20 0.654 0.1019 0.606 0.16 10 LYM1161 80178.4 0.0427 0.03 33 — — — — — — LYM116180179.1 0.0505 L 58 0.7811 0.04 42 — — — LYM1161 80179.3 — — — — — —0.617 0.11 12 LYM1157 82231.2 0.0364 0.12 14 — — — 0.614 0.13 12 LYM115782231.4 0.0543 L 69 0.827 L 51 — — — LYM1157 82232.2 0.0452 L 41 0.6700.15 22 — — — LYM1132 82013.1 0.0451 L 41 — — — 0.636 0.05 16 LYM113282013.4 0.0401 0.01 25 — — — 0.632 0.04 15 LYM1132 82017.6 0.0386 0.0621 — — — — — — LYM1124 82008.4 0.0398 0.02 24 0.713 0.03 30 — — —LYM1115 80133.1 0.0429 L 34 — — — 0.685 L 25 LYM1115 80133.6 0.0391 0.0422 0.629 0.29 15 — — — LYM1115 80134.2 0.0443 L 38 0.707 0.01 29 0.674 L23 LYM1115 80135.2 0.0563 L 76 0.834 0.04 52 0.612 0.17 11 LYM108582683.2 0.0558 L 74 0.728 0.12 33 — — — LYM1085 82683.3 0.0405 0.07 260.724 0.01 32 — — — LYM1085 82685.1 0.0476 L 48 0.724 0.04 32 0.663 0.0421 LYM1085 82685.12 0.0453 L 41 0.663 0.09 21 0.716 L 30 LYM1082 82481.20.0382 0.07 19 — — — — — — LYM1082 82481.4 — — — 0.629 0.18 15 — — —LYM1082 82482.4 — — — — — — 0.666 L 21 LYM1082 82482.5 0.0375 0.06 17 —— — — — — LYM1073 80978.3 0.0454 L 42 — — — — — — LYM1073 80978.4 0.0421L 31 — — — — — — LYM1073 80980.3 0.0369 0.12 15 — — — — — — LYM107380981.1 0.0463 L 44 — — — — — — LYM1054 80106.2 0.0405 L 26 — — — 0.661L 20 LYM1054 80108.2 0.0479 L 49 — — — — — — LYM1054 80110.4 0.0473 L 47— — — — — — LYM1044 82612.2 0.0468 L 46 — — — — — — LYM1044 82613.20.0372 0.11 16 — — — — — — LYM1044 82613.4 0.0430 L 34 0.726 L 32 0.6540.03 19 LYM1044 82614.1 0.0408 L 27 — — — — — — LYM1042 81998.2 0.03740.12 17 — — — 0.683 L 24 LYM1042 82000.2 0.0388 0.04 21 — — — — — —LYM1042 82000.4 0.0399 L 25 — — — 0.647 0.02 18 LYM1042 82001.2 0.03950.06 23 — — — — — — LYM1029 81349.4 0.0399 0.02 24 — — — 0.597 0.19 9LYM1029 81351.4 0.0429 L 34 0.824 L 50 0.696 L 27 LYM1029 81353.1 0.04100.02 28 0.628 0.23 14 0.620 0.13 13 LYM1029 81353.3 0.0393 0.08 23 0.6620.14 21 — — — CONT. — 0.0321 — — 0.549 — — 0.549 — — LYM1229 81574.10.0509 L 85 1.10 L 141 0.696 0.01 33 LYM1229 81574.2 0.0423 L 53 0.911 L100 0.662 0.06 27 LYM1229 81575.1 0.0417 L 51 0.674 0.05 48 — — —LYM1229 81575.3 0.0566 L 105 1.09 L 139 0.639 0.08 22 LYM1229 81576.50.0350 L 27 0.598 0.12 31 — — — LYM1227 81179.1 0.0384 L 39 0.672 0.0247 — — — LYM1227 81179.2 0.0466 L 69 0.878 L 93 0.632 0.11 21 LYM122781179.3 0.0341 0.02 24 0.660 0.04 45 0.603 0.25 15 LYM1227 81179.70.0462 L 67 0.837 0.01 84 — — — LYM1227 81180.2 0.0548 L 99 1.04 L 1280.675 0.03 29 LYM1219 80509.2 0.0520 L 89 0.796 L 75 0.655 0.06 25LYM1219 80510.3 0.0466 L 69 0.862 L 89 0.643 0.09 23 LYM1219 80510.40.0475 L 72 0.764 L 68 — — — LYM1219 80513.2 0.0529 L 92 0.989 L 1170.742 L 42 LYM1218 81114.1 0.0370 L 34 0.875 L 92 — — — LYM1218 81114.20.0679 L 146 1.12 L 145 0.660 0.08 26 LYM1218 81116.6 0.0330 0.04 200.668 0.03 47 0.609 0.20 17 LYM1218 81116.7 0.0512 0.01 86 0.977 L 1140.607 0.25 16 LYM1218 81116.8 0.0654 L 137 1.05 L 130 0.649 0.10 24LYM1216 80256.7 0.0565 L 105 1.15 L 153 0.672 0.05 29 LYM1216 80259.10.0462 L 67 0.740 L 62 0.650 0.07 25 LYM1216 80259.2 0.0454 L 65 0.798 L75 0.610 0.18 17 LYM1216 80260.1 0.0594 L 115 1.14 L 150 0.683 0.03 31LYM1212 80777.1 0.0392 L 42 0.593 0.13 30 0.619 0.19 19 LYM1212 80779.10.0466 L 69 — — — — — — LYM1210 80617.1 0.0344 0.01 25 0.659 0.04 450.604 0.22 16 LYM1210 80618.2 0.0413 L 50 0.678 0.04 49 — — — LYM121080618.3 0.0571 L 107 1.01 L 122 0.647 0.07 24 LYM1210 80620.2 0.0561 L103 0.872 L 91 0.687 0.02 32 LYM1210 80620.5 0.0494 L 79 0.710 0.04 56 —— — LYM1209 80479.1 0.0510 L 85 0.995 L 118 0.706 0.01 35 LYM120980480.1 0.0601 L 118 1.04 L 12.8 0.635 0.11 22 LYM1209 80482.3 0.0361 L31 0.563 0.23 24 — — — LYM1209 80482.4 0.0477 L 73 0.649 0.04 42 — — —LYM1209 80482.6 0.0437 L 58 0.810 L 78 0.592 0.30 13 LYM1201 81102.10.0403 L 46 0.791 L 73 0.718 L 37 LYM1201 81102.3 0.0351 0.02 27 0.6490.04 42 0.650 0.06 24 LYM1201 81103.1 0.0406 L 47 0.804 L 76 0.670 0.0328 LYM1201 81104.4 0.0540 L 96 0.794 L 74 — — — LYM1201 81105.2 0.0510 L85 0.818 L 80 0.651 0.06 25 LYM1195 81918.1 0.0317 0.17 15 — — — — — —LYM1195 81918.3 0.0542 L 97 1.23 L 170 0.769 L 47 LYM1195 81919.3 0.0558L 102 0.905 L 99 0.683 0.03 31 LYM1195 81919.4 0.0528 L 91 0.770 L 69 —— — LYM1195 81919.6 0.0505 L 83 0.806 L 77 0.672 0.08 29 LYM1189 81097.2— — — 0.605 0.13 33 — — — LYM1189 81099.1 0.0448 L 63 0.711 0.02 56 — —— LYM1189 81099.2 0.0437 L 58 0.876 L 92 0.668 0.04 28 LYM1189 81100.20.0381 L 38 0.730 0.02 60 0.635 0.12 22 LYM1189 81101.4 0.0388 L 410.589 0.18 29 0.653 0.08 25 LYM1182 81492.1 0.0502 L 82 0.696 0.03 530.622 0.20 19 LYM1182 81492.4 0.0736 L 167 1.39 L 206 0.716 0.01 37LYM1182 81493.2 0.0450 L 63 0.833 L 83 — — — LYM1182 81494.2 0.0373 L 350.808 L 77 0.602 0.26 15 LYM1182 81495.5 0.0467 L 69 0.834 L 83 0.6200.18 19 LYM1176 80810.3 0.0376 L 36 0.580 0.15 27 — — — LYM1176 80810.60.0452 L 64 0.701 0.02 54 0.617 0.15 18 LYM1176 80811.3 0.0497 L 800.998 L 119 0.615 0.29 18 LYM1176 80811.4 0.0353 0.01 28 0.741 0.03 630.628 0.19 20 LYM1173 80464.1 0.0727 L 164 1.69 L 272 0.745 L 43 LYM117380464.4 0.0599 L 117 1.18 L 159 0.634 0.14 21 LYM1173 80464.5 0.0452 L64 1.00 L 120 0.650 0.13 24 LYM1173 80466.2 0.0393 L 42 1.03 L 127 0.7100.02 36 LYM1170 80187.1 0.0439 L 59 0.664 0.04 46 0.615 0.26 18 LYM117080187.5 0.0452 L 64 0.702 0.02 54 0.665 0.05 27 LYM1170 80189.1 0.0413 L50 0.879 L 93 0.612 0.19 17 LYM1170 80189.3 0.0473 L 72 0.865 L 90 0.6220.24 19 LYM1170 80189.4 0.0459 L 66 0.929 L 104 0.687 0.02 32 LYM116980606.4 0.0516 L 87 0.907 L 99 0.608 0.28 16 LYM1169 80607.2 0.0312 0.2413 0.601 0.14 32 — — — LYM1169 80607.3 0.0452 L 64 1.01 L 121 0.672 0.0529 LYM1146 81487.4 0.0605 L 119 0.781 L 71 — — — LYM1146 81487.5 0.0441L 60 0.873 L 92 0.667 0.04 28 LYM1146 81488.2 0.0682 L 147 1.29 L 1840.730 L 40 LYM1146 81491.4 0.0501 L 82 0.589 0.14 29 — — — LYM114681491.5 0.0642 L 133 0.963 L 111 0.670 0.04 28 CONT. — 0.0276 — — 0.456— — 0.522 — — LYM1208 83928.4 0.0482 L 45 0.778 0.03 35 — — — LYM120883929.5 0.0406 0.06 22 — — — — — — LYM1208 83929.6 0.0495 L 49 0.7000.20 21 — — — LYM1205 83050.6 0.0566 L 70 0.894 L 55 — — — LYM120583054.3 0.0475 L 43 0.766 0.03 32 0.709 0.19 8 LYM1174 83274.1 0.0537 L62 0.910 0.01 57 — — — LYM1174 83274.5 0.0690 L 108 0.881 L 52 — — —LYM1160 81861.2 0.0402 0.15 21 0.785 0.09 36 — — — LYM1160 81862.30.0531 L 60 1.08 L 87 0.732 0.11 12 LYM1160 81862.5 — — — 0.735 0.09 270.720 0.15 10 LYM1153 83265.5 0.0522 L 57 0.862 0.02 49 — — — LYM115383267.2 0.0430 0.02 29 — — — — — — LYM1153 83267.4 0.0531 L 60 0.873 L51 — — — LYM1153 83267.5 0.0495 L 49 — — — — — — LYM1125 83041.4 0.0550L 65 0.820 0.05 42 — — — LYM1125 83042.1 0.0398 0.07 20 0.669 0.27 16 —— — LYM1125 83042.5 0.0571 L 72 0.698 0.28 21 — — — LYM1122 81983.50.0381 0.28 15 — — — — — — LYM1122 81983.6 0.0611 L 84 0.967 L 67 — — —LYM1122 81985.2 0.0461 L 39 0.766 0.03 33 — — — LYM1122 81985.4 0.0475 L43 — — — — — — LYM1122 81985.5 0.0474 L 42 0.686 0.24 19 — — — LYM109083377.1 0.0456 0.02 37 0.782 0.03 35 — — — LYM1090 83379.3 0.0507 L 53 —— — — — — LYM1090 83379.4 0.0417 0.04 25 0.802 0.03 39 — — — LYM109083379.6 0.0478 L 44 0.772 0.05 34 — — — LYM1088 83557.1 0.0690 L 1071.17 L 102 — — — LYM1088 83557.2 0.0563 L 69 0.983 L 70 0.717 0.15 10LYM1088 83558.1 0.0467 0.02 40 0.893 0.01 55 — — — LYM1088 83558.40.0615 L 85 1.04 L 80 0.733 0.17 12 LYM1088 83558.5 0.0425 0.02 28 0.7250.07 25 — — — LYM1046 81907.2 0.0418 0.05 26 — — — — — — LYM1046 81907.40.0383 0.22 15 — — — — — — LYM1046 81909.2 0.0398 0.09 20 — — — — — —LYM1046 81909.5 0.0531 L 60 — — — — — — LYM1035 83260.3 0.0493 0.03 480.941 0.03 63 — — — LYM1035 83260.5 0.0510 L 53 0.933 L 61 — — — LYM103583262.1 0.0494 L 49 0.867 L 50 — — — LYM1035 83264.6 — — — 0.695 0.28 20— — — LYM1030 83544.3 0.0377 0.22 13 — — — — — — LYM1030 83547.2 0.04300.02 29 0.852 L 47 — — — CONT. — 0.0332 — — 0.578 — — 0.654 — — LYM111882534.6 0.0443 0.13 30 — — — — — — LYM1114 82487.11 0.0436 0.11 28 — — —— — — LYM1114 82487.7 0.0489 0.03 43 0.720 0.07 46 — — — LYM1113 81872.40.0498 0.01 46 0.731 0.04 48 0.721 0.03 31 LYM1106 82627.2 0.0538 0.0258 0.724 0.06 47 — — — LYM1106 82627.3 — — — 0.620 0.24 26 — — — LYM107882657.6 0.0434 0.14 27 — — — — — — LYM1078 82657.9 0.0521 0.01 53 0.854L 73 0.656 0.23 20 LYM1078 82659.2 0.0558 L 64 0.830 L 68 0.664 0.13 21LYM1078 82659.5 0.0427 0.20 25 — — — — — — LYM1075 83047.2 0.0474 0.0339 0.663 0.12 34 0.637 0.26 16 LYM1075 83047.8 0.0427 0.19 25 — — — — —— LYM1075 83049.1 0.0495 0.03 45 0.770 0.03 56 0.664 0.15 21 LYM107583049.3 0.0521 0.02 53 0.780 0.03 58 — — — LYM1074 82572.2 0.0444 0.0830 — — — — — — LYM1033 82883.6 0.0449 0.08 32 0.787 0.01 60 0.639 0.2416 LYM1033 82885.5 0.0406 0.26 19 0.683 0.09 39 — — — LYM1027 82520.30.0580 L 70 0.789 0.02 60 — — — LYM1027 82520.6 0.0463 0.08 36 — — — — —— LYM1024 82475.5 0.0423 0.18 24 — — — — — — LYM1023 82516.1 0.0533 0.0157 0.718 0.12 46 — — — LYM1023 82518.10 0.0433 0.14 27 — — — — — — CONT.— 0.0341 — — 0.493 — — 0.549 — — Table 118. “CONT.”-Control;“Ave.”-Average; “% Incr.” = % increment; “p-val.”- p-value, L-p < 0.01.Results from T1 Plants

Tables 119-121 summarize the observed phenotypes of transgenic plantsexpressing the gene constructs using the TC-T1 Assays.

The genes presented in Tables 119-121 showed a significant improvementin plant biomass and root development since they produced a higherbiomass (dry and fresh weight, Table 119), a larger leaf and rootbiomass (leaf area, root length and root coverage) (Table 120), and ahigher relative growth rate of leaf area, root coverage and root length(Table 121) when grown under normal growth conditions, compared tocontrol plants grown under identical growth conditions. Plants producinglarger root biomass have better possibilities to absorb larger amount ofnitrogen from soil. Plants producing larger leaf biomass has betterability to produce assimilates). The genes were cloned under theregulation of a constitutive promoter (At6669; SEQ ID NO: 8190). Theevaluation of each gene was performed by testing the performance ofdifferent number of events. Some of the genes were evaluated in morethan one tissue culture assay. This second experiment confirmed thesignificant increment in leaf and root performance. Event with p-value<0.1 was considered statistically significant.

TABLE 119 Genes showing improved plant performance at Normal growthconditions under regulation of At6669 promoter Dry Weight [mg] FreshWeight [mg] Gene Name Ave. P-Val. % Incr. Ave. P-Val. % Incr. LYM1164_H19.15 0.25 44 — — — LYM1018 8.00 L 26 234.9 0.29 41 CONT. 6.33 — — 167.0— — LYM1052 10.0 0.14 13 210.8 0.27 27 CONT. 8.84 — — 165.4 — — Table119. “CONT.”-Control; “Ave.”-Average; “% Incr.” = % increment; “p-val.”-p-value, L-p < 0.01.

TABLE 120 Genes showing improved plant performance at Normal growthconditions under regulation of At6669 promoter Leaf Area [cm2] RootsCoverage [cm²] Roots Length [cm] Gene Name Ave. P-Val. % Incr. Ave.P-Val. % Incr. Ave. P-Val. % Incr. LYM1164_H1 0.870 0.12 31 9.35 0.02 487.64 0.19 18 LYM1018 0.772 0.05 17 — — — — — — CONT. 0.661 — — 6.33 — —6.45 — — Table 120. “CONT.”-Control; “Ave.”-Average; “% Incr.” = %increment; “p-val.”- p-value, L-p < 0.01

TABLE 121 Genes showing improved plant performance at Normal growthconditions under regulation of At6669 promoter RGR Of RGR Of Roots RGROf Leaf Area Coverage Root Length Gene Name Ave. P-Val. % Incr. Ave.P-Val. % Incr. Ave. P-Val. % Incr. LYM1164_H1 0.0829 0.03 38 1.12 L 490.807 0.08 22 LYM1018 0.0708 0.26 18 — — — — — — CONT. 0.0603 — — 0.754— — 0.662 — — Table 121. “CONT.”-Control; “Ave.”-Average; “% Incr.” = %increment; “p-val.”- p-value, L-p < 0.01

These results demonstrate that the polynucleotides of the invention arecapable of improving yield and additional valuable importantagricultural traits such as increase of biomass, abiotic stresstolerance, nitrogen use efficiency, yield, vigor, fiber yield and/orquality. Thus, transformed plants showing improved fresh and dry weightdemonstrate the gene capacity to improve biomass a key trait of cropsfor forage and plant productivity: transformed plants showingimprovement of seed yield demonstrate the genes capacity to improveplant productivity; transformed plants showing improvement of plotcoverage and rosette diameter demonstrate the genes capacity to improveplant drought resistance as they reduce the loss of soil water by simpleevaporation and reduce the competition with weeds; hence reduce the needto use herbicides to control weeds. Transformed plants showingimprovement of relative growth rate of various organs (leaf and root)demonstrate the gene capacity to promote plant growth and henceshortening the needed growth period and/or alternatively improving theutilization of available nutrients and water leading to increase of landproductivity; Transformed plants showing improvement of organ number asdemonstrated by the leaf number parameter exhibit a potential to improvebiomass yield important for forage crops and improve the plantproductivity; Transformed plants showing increased root length andcoverage demonstrate the gene capacity to improve drought resistance andbetter utilization of fertilizers as the roots can reach larger soilvolume; Transformed plants showing improvement of leaf petiole relativearea and leaf blade area demonstrate the genes capacity to cope withlimited light intensities results from increasing the plant populationdensities and hence improve land productivity.

Example 22 Evaluation of Transgenic Brachypodium Nue and Yield Under Lowor Normal Nitrogen Fertilization in Greenhouse Assay

Assay 3: Nitrogen Use efficiency measured plant biomass and yield atlimited and optimal nitrogen concentration under greenhouse conditionsuntil heading—This assay follows the plant biomass formation and growth(measured by height) of plants which were grown in the greenhouse atlimiting and non-limiting (e.g., normal) nitrogen growth conditions.Transgenic Brachypodium seeds were sown in peat plugs. The T₁ transgenicseedlings were then transplanted to 27.8×11.8×8.5 cm trays filled withpeat and perlite in a 1:1 ratio. The trays were irrigated with asolution containing nitrogen limiting conditions, which were achieved byirrigating the plants with a solution containing 3 mM inorganic nitrogenin the form of NH₄NO₃, supplemented with 1 mM KH₂PO₄, 1 mM MgSO₄. 3.6 mMKCl, 2 mM CaCl₂) and microelements, while normal nitrogen levels wereachieved by applying a solution of 6 mM inorganic nitrogen also in theform of NH₄NO₃ with 1 mM KH₂PO₄, 1 mM MgSO₄, 2 mM CaC₂. 3.6 mM KCl andmicroelements. All plants were grown in the greenhouse until heading.Plant biomass (the above ground tissue) was weighted right afterharvesting the shoots (plant fresh weight [FW]). Following, plants weredried in an oven at 70° C. for 48 hours and weighed (plant dry weight[DW]).

Each construct was validated at its T₁ generation. Transgenic plantstransformed with a construct conformed by an empty vector carrying theBASTA selectable marker were used as control (FIG. 9B).

The plants were analyzed for their overall size, fresh weight and drymatter. Transgenic plants performance was compared to control plantsgrown in parallel under the same conditions. Mock-transgenic plants withno gene and no promoter at all, were used as control (FIG. 9B).

The experiment was planned in blocks and nested randomized plotdistribution within them. For each gene of the invention fiveindependent transformation events were analyzed from each construct.

Phenotyping

Plant Fresh and Dry shoot weight—In Heading assays when heading stagehas completed (about day 30 from sowing), the plants were harvested anddirectly weighed for the determination of the plant fresh weight onsemi-analytical scales (0.0.01 gr) (FW) and left to dry at 70° C. in adrying chamber for about 48 hours before weighting to determine plantdry weight (DW).

Time to Heading—In both Seed Maturation and Heading assays heading wasdefined as the full appearance of the first spikelet in the plant. Thetime to heading occurrence is defined by the date the heading iscompletely visible. The time to heading occurrence date was documentedfor all plants and then the time from planting to heading wascalculated.

Leaf thickness—In Heading assays when minimum 5 plants per plot in atleast 90% of the plots in an experiment have been documented at heading,measurement of leaf thickness was performed using a micro-meter on thesecond leaf below the flag leaf.

Plant Height—In both Seed Maturation and Heading assays once heading wascompletely visible, the height of the first spikelet was measured fromsoil level to the bottom of the spikelet.

Tillers number—In Heading assays manual count of tillers was preformedper plant after harvest, before weighing.

Example 23 Evaluation of Transgenic Brachypodium NUE and Yield Under Lowor Normal Nitrogen Fertilization in Greenhouse Assay

Assay 4: Nitrogen Use efficiency measured plant biomass and yield atlimited and optimal nitrogen concentration under greenhouse conditionsuntil Seed Maturation—This assay follows the plant biomass and yieldproduction of plants that were grown in the greenhouse at limiting andnon-limiting nitrogen growth conditions. Transgenic Brachypodium seedswere sown in peat plugs. The T₁ transgenic seedlings were thentransplanted to 27.8×11.8×8.5 cm trays filled with peat and perlite in a1:1 ratio. The trays were irrigated with a solution containing nitrogenlimiting conditions, which were achieved by irrigating the plants with asolution containing 3 mM inorganic nitrogen in the form of NH₄NO₃,supplemented with 1 mM KH₂PO₄. 1 mM MgSO₄. 3.6 mM KCl, 2 mM CaCl₂) andmicroelements, while normal nitrogen levels were achieved by applying asolution of 6 mM inorganic nitrogen also in the form of NH₄NO₃ with 1 mMKH₂PO₄. 1 mM MgSO₄. 2 mM CaCl₂), 3.6 mM KCl and microelements. Allplants were grown in the greenhouse until seed maturation. Eachconstruct was validated at its T₁ generation. Transgenic plantstransformed with a construct conformed by an empty vector carrying theBASTA selectable marker were used as control (FIG. 9B).

The plants were analyzed for their overall biomass, fresh weight and drymatter, as well as a large number of yield and yield components relatedparameters. Transgenic plants performance was compared to control plantsgrown in parallel under the same conditions. Mock-transgenic plants withno gene and no promoter at all (FIG. 9B). The experiment was planned inblocks and nested randomized plot distribution within them. For eachgene of the invention five independent transformation events wereanalyzed from each construct.

Phenotyping

Plant Fresh and Dry vegetative weight—In Seed Maturation assays whenmaturity stage has completed (about day 80 from sowing), the plants wereharvested and directly weighed for the determination of the plant freshweight (FW) and left to dry at 70° C. in a drying chamber for about 48hours before weighting to determine plant dry weight (DW).

Spikelets Dry weight (SDW)—In Seed Maturation assays when maturity stagehas completed (about day 80 from sowing), the spikelets were separatedfrom the biomass, left to dry at 70° C. in a drying chamber for about 48hours before weighting to determine spikelets dry weight (SDW).

Grain Yield per Plant—In Seed Maturation assays after drying ofspikelets for SDW, spikelets were run through production machine, thenthrough cleaning machine, until seeds were produced per plot, thenweighed and Grain Yield per Plant was calculated.

Grain Number—In Seed Maturation assays after seeds per plot wereproduced and cleaned, the seeds were run through a counting machine andcounted.

1000 Seed Weight—In Seed Maturation assays after seed production, afraction was taken from each sample (seeds per plot; ˜0.5 gr), countedand photographed, 1000 seed weight was calculated.

Harvest Index—In Seed Maturation assays after seed production, harvestindex was calculated by dividing grain yield and vegetative dry weight.

Time to Heading—In both Seed Maturation and Heading assays heading wasdefined as the full appearance of the first spikelet in the plant. Thetime to heading occurrence is defined by the date the heading iscompletely visible. The time to heading occurrence date was documentedfor all plants and then the time from planting to heading wascalculated.

Leaf thickness—In Heading assays when minimum 5 plants per plot in atleast 90% of the plots in an experiment have been documented at heading,measurement of leaf thickness was performed using a micro-meter on thesecond leaf below the flag leaf.

Grain filling period—In Seed Maturation assays maturation was defined bythe first color-break of spikelet+stem on the plant, from green toyellow/brown.

Plant Height—In both Seed Maturation and Heading assays once heading wascompletely visible, the height of the first spikelet was measured fromsoil level to the bottom of the spikelet.

Tillers number—In Heading assays manual count of tillers was preformedper plant after harvest, before weighing.

Number of reproductive heads per plant—In Heading assays manual count ofheads per plant was performed.

Statistical analyses—To identify genes conferring significantly improvedtolerance to abiotic stresses, the results obtained from the transgenicplants were compared to those obtained from control plants. To identifyoutperforming genes and constructs, results from the independenttransformation events tested were analyzed separately. Data was analyzedusing Student's t-test and results were considered significant if the pvalue was less than 0.1. The JMP statistics software package was used(Version 5.2.1. SAS Institute Inc., Cary. N.C., USA).

Although the invention has been described in conjunction with specificembodiments thereof, it is evident that many alternatives, modificationsand variations will be apparent to those skilled in the art.Accordingly, it is intended to embrace all such alternatives,modifications and variations that fall within the spirit and broad scopeof the appended claims.

It is the intent of the applicant(s) that all publications, patents andpatent applications referred to in this specification are to beincorporated in their entirety by reference into the specification, asif each individual publication, patent or patent application wasspecifically and individually noted when referenced that it is to beincorporated herein by reference. In addition, citation oridentification of any reference in this application shall not beconstrued as an admission that such reference is available as prior artto the present invention. To the extent that section headings are used,they should not be construed as necessarily limiting. In addition, anypriority document(s) of this application is/are hereby incorporatedherein by reference in its/their entirety.

What is claimed is:
 1. A method of increasing yield, harvest index,growth rate, biomass, vigor, oil content, seed yield, fiber yield, fiberquality, fiber length, photosynthetic capacity, nitrogen use efficiency,and/or abiotic stress tolerance, and/or reducing time to floweringand/or to inflorescence emergence of a plant, comprising transforming aplant with a heterologous nucleic acid sequence encoding a polypeptidecomprising an amino acid sequence at least 80% identical to the aminoacid sequence set forth by SEQ ID NO: 753, thereby increasing the yield,harvest index, growth rate, biomass, vigor, oil content, seed yield,fiber yield, fiber quality, fiber length, photosynthetic capacity,nitrogen use efficiency, and/or abiotic stress tolerance, and/orreducing the time to flowering and/or to inflorescence emergence of theplant.
 2. The method of claim 1, wherein said amino acid sequence is atleast 95% identical to the amino acid sequence set forth by SEQ ID NO:753.
 3. The method of claim 1, wherein said polypeptide is selected fromthe group consisting of SEQ ID NOs: 753 and
 648. 4. The method of claim1, wherein said heterologous nucleic acid sequence is selected from thegroup consisting of SEQ ID NOs: 429 and
 175. 5. The method of claim 1,further comprising growing the plant transformed with said heterologousnucleic acid sequence under the abiotic stress.
 6. The method of claim1, wherein said abiotic stress is selected from the group consisting ofsalinity, drought, osmotic stress, water deprivation, flood, etiolation,low temperature, high temperature, heavy metal toxicity, anaerobiosis,nutrient deficiency, nitrogen deficiency, nutrient excess, atmosphericpollution and UV irradiation.
 7. The method of claim 1, furthercomprising growing the plant transformed with said heterologous nucleicacid sequence under nitrogen-limiting conditions.
 8. The method of claim1, further comprising selecting said plant transformed with saidheterologous nucleic acid sequence for an increased yield, harvestindex, growth rate, biomass, vigor, oil content, seed yield, fiberyield, fiber quality, fiber length, photosynthetic capacity, nitrogenuse efficiency, and/or abiotic stress tolerance, and/or for a reducedtime to flowering and/or a reduced time to inflorescence emergence ascompared to the wild type plant of the same species which is grown underthe same growth conditions.
 9. The method of claim 8, wherein saidselecting is performed under non-stress conditions.
 10. The method ofclaim 8, wherein said selecting is performed under abiotic stressconditions.
 11. A method of producing a crop, comprising growing a cropplant transformed with an exogenous polynucleotide comprising a nucleicacid sequence encoding a polypeptide at least 80% identical to the aminoacid sequence set forth by SEQ ID NO: 753, wherein the crop plant isderived from plants which have been transformed with said exogenouspolynucleotide and which have been selected for increased yield,increased harvest index, increased growth rate, increased biomass,increased vigor, increased oil content, increased seed yield, increasedfiber yield, increased fiber quality, increased fiber length, increasedphotosynthetic capacity, increased nitrogen use efficiency, increasedabiotic stress tolerance, reduced time to flowering and/or reduced timeto inflorescence emergence as compared to a wild type plant of the samespecies which is grown under the same growth conditions, and the cropplant has the increased yield, increased harvest index, increased growthrate, increased biomass, increased vigor, increased oil content,increased seed yield, increased fiber yield, increased fiber quality,increased fiber length, increased photosynthetic capacity, increasednitrogen use efficiency, increased abiotic stress tolerance, reducedtime to flowering and/or reduced time to inflorescence emergence,thereby producing the crop.
 12. The method of claim 11, wherein saidamino acid sequence is at least 95% identical to the amino acid sequenceset forth by SEQ ID NO:
 753. 13. The method of claim 11, wherein saidexogenous polynucleotide comprises a nucleic acid sequence selected fromthe group consisting of SEQ ID NOs: 753 and
 648. 14. A nucleic acidconstruct comprising an isolated polynucleotide which comprises anucleic acid sequence encoding a polypeptide comprising an amino acidsequence at least 80% identical to the amino acid sequence set forth inSEQ ID NO: 753, and a heterologous promoter for directing transcriptionof said nucleic acid sequence in a host cell, wherein said amino acidsequence is capable of increasing yield, harvest index, growth rate,biomass, vigor, oil content, seed yield, fiber yield, fiber quality,fiber length, photosynthetic capacity, nitrogen use efficiency, and/orabiotic stress tolerance, and/or reducing time to flowering and/or toinflorescence emergence of a plant.
 15. The nucleic acid construct ofclaim 14, wherein said polypeptide is selected from the group consistingof SEQ ID NOs: 753 and
 648. 16. The nucleic acid construct of claim 14,wherein said nucleic acid sequence is selected from the group consistingof SEQ ID NOs: 429 and
 175. 17. A plant cell transformed with thenucleic acid construct of claim
 14. 18. The plant cell of claim 17,wherein said plant cell forms part of a plant.
 19. A transgenic plantcomprising the nucleic acid construct of claim
 14. 20. A method ofgrowing a crop, the method comprising seeding seeds and/or plantingplantlets of a plant transformed with the nucleic acid construct ofclaim 14, wherein the plant is derived from plants which have beentransformed with said nucleic acid construct and which have beenselected for at least one trait selected from the group consisting of:increased nitrogen use efficiency, increased abiotic stress tolerance,increased biomass, increased growth rate, increased vigor increasedyield, increased harvest index, increased fiber yield, increased fiberquality, increased fiber length, increased photosynthetic capacity,increased oil content, reduced time to flowering and reduced time toinflorescence emergence as compared to a non-transformed plant, therebygrowing the crop.
 21. A method of selecting a transformed plant havingincreased yield, harvest index, growth rate, biomass, vigor, oilcontent, seed yield, fiber yield, fiber quality, fiber length,photosynthetic capacity, nitrogen use efficiency, and/or abiotic stresstolerance, and/or reducing time to flowering and/or to inflorescenceemergence as compared to a wild type plant of the same species which isgrown under the same growth conditions, the method comprising: (a)transforming a plant with the nucleic acid construct of claim 14, (b)selecting said plants of step (a) for an increased yield, harvest index,growth rate, biomass, vigor, oil content, seed yield, fiber yield, fiberquality, fiber length, photosynthetic capacity, nitrogen use efficiency,and/or abiotic stress tolerance, and/or for a reduced time to floweringand/or to inflorescence emergence as compared to a wild type plant ofthe same species which is grown under the same growth conditions,thereby selecting the plant having the increased yield, harvest index,growth rate, biomass, vigor, oil content, seed yield, fiber yield, fiberquality, fiber length, photosynthetic capacity, nitrogen use efficiency,and/or abiotic stress tolerance, and/or the reduced time to floweringand/or the reduced time to inflorescence emergence as compared to thewild type plant of the same species which is grown under the same growthconditions.